Rw4G033700

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014915
Physical Location & Seq
Forward (+)
60827438 .. 60829677
2240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G033700.1

Sequence Viewer

Length: 1017 bp
ATGAATGCAAACCTTCTGTTCTTCTCTGCTCTGTTTCTTGTACTTGTAAGCTGCTGCAGCTTATCCTCTTGTGCAGCAGATGCAGATCATGAGCTACGCCGGCTGCAACACGCTTCCGCCAAAGCCGACGGATCTCTGAGCTTCTTGGTCGTTGGAGATTGGGGAAGAAGAGGAACTTACAATCAATCCCGAGTTGCTCTTCAGATGGGACTAATTGGAGAGAAGTTGGATATCGATTTTGTAATATCCACCGGCGATAACTTTTACGATGATGGATTGACGGGCATCGACGATCCGGCCTTTGAAGAGTCGTTTTCCAAGATCTACCCAGCCCCTAGCTTGCAAAAGCAATGGTACAATGTTTTGGGTAATCATGATTATAGGGGTAATGTTGAGGCCCAATTAAGTCCTGTCCTGACTGGATTGGATGGTAGATGGCTTTGCATGAGATCCTTCATAGTTGATGCAGAAATTGCAGAGTTTTTCTTCGTGGACACAACTCCCTTTGCAGACAAATACTTCACTGACCCAGAGGACCATGTCTACGACTGGAGTGGCATATTACCCAGAAATGAGTACCTCTCAAATATTCTCAAGGACGTGGATTCAACGCTAAAAGAATCGAACGCGAAGTGGAAGATCGTGGTCGGTCACCATGGAATTAGAAGTGCTGGACAACATGGTGACACTCCAGAGCTTGTATCACAGCTTCTCCCAATTCTTGAGGCCAACGATGTTGATCTTTACATCAATGGGCACGACCATTGCTTACAACACATTAGTACTCCCAACAGCCCGATTCAATTTCTAACGAGTGGTGGTGGATCGAAGGCATGGAGAGGCGTTGTAATGCCGTACGATCCAAGTGAAATAAAGCTGTATTACGACGGACAGGGTTTCATGTCAGTGCAAATCACTCCAAGCCAAGTAGATATTGCATTCTATGATGTTTTTGGTAATGTGTTGCACAAATGGGGCACATCAAAGGACCTTTACTCTACTACCATGTATATGTAG

Protein Analysis

338

Amino Acids

37.75

Weight (kDa)

4.79

Isoelectric Point (pI)

46.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 47 - 256 7.8e-20 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014915)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g33011 FvH4_4g33011
malus_domestica MD13G1016000.v1.1 MD16G1013600.v1.1
prunus_persica Prupe.1G337600_v2.0.a1
pyrus_communis pycom13g01370
rosa_chinensis RchiOBHm_Chr4g0442051
rosa_laevigata RLG00000006048
rosa_multiflora Rmu_sc0002501.1_g000009
rosa_roxburghii Rroxscaffold_5G00382970
rosa_rugosa Rorug04G0338300
rosa_samantha Rh4AG390000 Rh4BG403400 Rh4CG418500 Rh4DG396600
rosa_wichuraiana Rw4G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 543
AccII CGCG 1 cut(s) 629
AciI CCGC 1 cut(s) 117
AclWI GGATC 5 cut(s) 139, 287, 444, 832, 854
AcuI CTGAAG 1 cut(s) 185
AfaI GTAC 5 cut(s) 42, 356, 578, 784, 857
AgsI TTSAA 3 cut(s) 305, 609, 803
AjiI CACGTC 1 cut(s) 601
AluBI AGCT 8 cut(s) 51, 60, 94, 141, 339, 697, 709, 877
AluI AGCT 8 cut(s) 51, 60, 94, 141, 339, 697, 709, 877
AlwI GGATC 5 cut(s) 139, 287, 444, 832, 854
Ama87I CYCGRG 1 cut(s) 189
AoxI GGCC 3 cut(s) 297, 396, 726
ApeKI GCWGC 5 cut(s) 51, 54, 57, 74, 103
AspS9I GGNCC 3 cut(s) 397, 535, 988
AsuHPI GGTGA 2 cut(s) 644, 695
AvaI CYCGRG 1 cut(s) 189
AvaII GGWCC 2 cut(s) 535, 988
BaeGI GKGCMC 2 cut(s) 759, 980
BarI GAAGNNNNNNTAC 2 cut(s) 693, 725
BbvI GCAGC 5 cut(s) 38, 41, 69, 86, 90
BccI CCATC 4 cut(s) 199, 266, 422, 429
BceAI ACGGC 1 cut(s) 838
BfaI CTAG 1 cut(s) 336
BfmI CTRYAG 1 cut(s) 55
BglII AGATCT 1 cut(s) 321
BisI GCNGC 5 cut(s) 52, 55, 58, 75, 104
BlsI GCNGC 5 cut(s) 53, 56, 59, 76, 105
BmcAI AGTACT 1 cut(s) 784
Bme18I GGWCC 2 cut(s) 535, 988
BmeT110I CYCGRG 1 cut(s) 189
BmgBI CACGTC 1 cut(s) 601
BmgT120I GGNCC 3 cut(s) 397, 535, 988
BmsI GCATC 3 cut(s) 70, 294, 454
BplI GAGNNNNNCTC 2 cut(s) 566, 598
BpmI CTGGAG 2 cut(s) 571, 675
BpuEI CTTGAG 2 cut(s) 578, 743
Bsa29I ATCGAT 1 cut(s) 234
BsaBI GATNNNNATC 2 cut(s) 84, 738
BsaJI CCNNGG 1 cut(s) 655
BsaXI ACNNNNNCTCC 2 cut(s) 696, 726
Bse118I RCCGGY 2 cut(s) 99, 251
Bse1I ACTGG 2 cut(s) 424, 554
Bse3DI GCAATG 2 cut(s) 356, 763
Bse8I GATNNNNATC 2 cut(s) 84, 738
BseCI ATCGAT 1 cut(s) 234
BseDI CCNNGG 1 cut(s) 655
BseGI GGATG 1 cut(s) 433
BseJI GATNNNNATC 2 cut(s) 84, 738
BseMI GCAATG 2 cut(s) 356, 763
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 2 cut(s) 424, 554
BseSI GKGCMC 2 cut(s) 759, 980
BseXI GCAGC 5 cut(s) 38, 41, 69, 86, 90
BseYI CCCAGC 1 cut(s) 328
BsgI GTGCAG 1 cut(s) 93
Bsh1236I CGCG 1 cut(s) 629
BshFI GGCC 3 cut(s) 299, 398, 728
BshVI ATCGAT 1 cut(s) 234
BsiHKCI CYCGRG 1 cut(s) 189
BsiSI CCGG 3 cut(s) 100, 252, 296
BsiWI CGTACG 1 cut(s) 855
BslFI GGGAC 1 cut(s) 222
BsmFI GGGAC 1 cut(s) 222
BsmI GAATGC 2 cut(s) 10, 938
BsnI GGCC 3 cut(s) 299, 398, 728
BsoBI CYCGRG 1 cut(s) 189
Bsp1286I GDGCHC 2 cut(s) 759, 980
Bsp143I GATC 9 cut(s) 85, 131, 292, 321, 449, 639, 739, 824, 859
Bsp19I CCATGG 1 cut(s) 655
BspACI CCGC 1 cut(s) 117
BspANI GGCC 3 cut(s) 299, 398, 728
BspCNI CTCAG 1 cut(s) 129
BspDI ATCGAT 1 cut(s) 234
BspFNI CGCG 1 cut(s) 629
BspHI TCATGA 2 cut(s) 88, 373
BspMAI CTGCAG 1 cut(s) 59
BspPI GGATC 5 cut(s) 139, 287, 444, 832, 854
BspQI GCTCTTC 1 cut(s) 204
BsrDI GCAATG 2 cut(s) 356, 763
BsrFI RCCGGY 2 cut(s) 99, 251
BsrI ACTGG 2 cut(s) 424, 554
BssAI RCCGGY 2 cut(s) 99, 251
BssECI CCNNGG 1 cut(s) 655
BssMI GATC 9 cut(s) 85, 131, 292, 321, 449, 639, 739, 824, 859
BssT1I CCWWGG 1 cut(s) 655
Bst6I CTCTTC 3 cut(s) 163, 204, 300
BstAPI GCANNNNNTGC 2 cut(s) 80, 473
BstC8I GCNNGC 2 cut(s) 101, 341
BstDEI CTNAG 1 cut(s) 137
BstDSI CCRYGG 1 cut(s) 655
BstEII GGTNACC 1 cut(s) 650
BstF5I GGATG 1 cut(s) 433
BstFNI CGCG 1 cut(s) 629
BstKTI GATC 9 cut(s) 88, 134, 295, 324, 452, 642, 742, 827, 862
BstMBI GATC 9 cut(s) 85, 131, 292, 321, 449, 639, 739, 824, 859
BstMWI GCNNNNNNNGC 4 cut(s) 57, 80, 100, 473
BstPI GGTNACC 1 cut(s) 650
BstSFI CTRYAG 1 cut(s) 55
BstSLI GKGCMC 2 cut(s) 759, 980
BstUI CGCG 1 cut(s) 629
BstV1I GCAGC 5 cut(s) 38, 41, 69, 86, 90
BstX2I RGATCY 3 cut(s) 131, 321, 449
BstYI RGATCY 3 cut(s) 131, 321, 449
Bsu15I ATCGAT 1 cut(s) 234
BsuRI GGCC 3 cut(s) 299, 398, 728
BsuTUI ATCGAT 1 cut(s) 234
BtgI CCRYGG 1 cut(s) 655
BtrI CACGTC 1 cut(s) 601
BtsCI GGATG 1 cut(s) 433
BtsIMutI CAGTG 2 cut(s) 522, 912
Cac8I GCNNGC 2 cut(s) 101, 341
CciI TCATGA 2 cut(s) 88, 373
Cfr10I RCCGGY 2 cut(s) 99, 251
Cfr13I GGNCC 3 cut(s) 397, 535, 988
ClaI ATCGAT 1 cut(s) 234
Csp6I GTAC 5 cut(s) 41, 355, 577, 783, 856
CviAII CATG 9 cut(s) 89, 374, 445, 539, 656, 680, 834, 901, 1006
CviQI GTAC 5 cut(s) 41, 355, 577, 783, 856
DdeI CTNAG 1 cut(s) 137
DpnI GATC 9 cut(s) 87, 133, 294, 323, 451, 641, 741, 826, 861
DpnII GATC 9 cut(s) 85, 131, 292, 321, 449, 639, 739, 824, 859
Eam1104I CTCTTC 3 cut(s) 163, 204, 300
EarI CTCTTC 3 cut(s) 163, 204, 300
EciI GGCGGA 1 cut(s) 106
Eco130I CCWWGG 1 cut(s) 655
Eco32I GATATC 1 cut(s) 232
Eco47I GGWCC 2 cut(s) 535, 988
Eco57I CTGAAG 1 cut(s) 185
Eco88I CYCGRG 1 cut(s) 189
Eco91I GGTNACC 1 cut(s) 650
EcoO109I RGGNCCY 1 cut(s) 988
EcoO65I GGTNACC 1 cut(s) 650
EcoRV GATATC 1 cut(s) 232
EcoT14I CCWWGG 1 cut(s) 655
ErhI CCWWGG 1 cut(s) 655
FaeI CATG 9 cut(s) 92, 377, 448, 542, 659, 683, 837, 904, 1009
FalI AAGNNNNNCTT 2 cut(s) 160, 192
FaqI GGGAC 1 cut(s) 222
FatI CATG 9 cut(s) 88, 373, 444, 538, 655, 679, 833, 900, 1005
FblI GTMKAC 1 cut(s) 543
Fnu4HI GCNGC 5 cut(s) 52, 55, 58, 75, 104
FokI GGATG 1 cut(s) 440
Fsp4HI GCNGC 5 cut(s) 52, 55, 58, 75, 104
FspBI CTAG 1 cut(s) 336
GluI GCNGC 5 cut(s) 52, 55, 58, 75, 104
GsaI CCCAGC 1 cut(s) 332
GsuI CTGGAG 2 cut(s) 571, 675
HaeIII GGCC 3 cut(s) 299, 398, 728
HapII CCGG 3 cut(s) 100, 252, 296
Hin1II CATG 9 cut(s) 92, 377, 448, 542, 659, 683, 837, 904, 1009
HinfI GANTC 4 cut(s) 308, 605, 620, 799
HpaII CCGG 3 cut(s) 100, 252, 296
HphI GGTGA 2 cut(s) 644, 695
Hpy166II GTNNAC 2 cut(s) 493, 544
Hpy188I TCNGA 2 cut(s) 138, 204
Hpy188III TCNNGA 6 cut(s) 89, 189, 374, 415, 692, 722
Hpy8I GTNNAC 2 cut(s) 493, 544
Hpy99I CGWCG 3 cut(s) 131, 293, 890
HpyAV CCTTC 3 cut(s) 23, 463, 823
HpyCH4IV ACGT 1 cut(s) 600
HpyF10VI GCNNNNNNNGC 4 cut(s) 57, 80, 100, 473
HpyF3I CTNAG 1 cut(s) 137
HpySE526I ACGT 1 cut(s) 600
Hsp92II CATG 9 cut(s) 92, 377, 448, 542, 659, 683, 837, 904, 1009
KroI GCCGGC 1 cut(s) 99
KroNI GCCGGC 1 cut(s) 101
Kzo9I GATC 9 cut(s) 85, 131, 292, 321, 449, 639, 739, 824, 859
LguI GCTCTTC 1 cut(s) 204
Lsp1109I GCAGC 5 cut(s) 38, 41, 69, 86, 90
LweI GCATC 3 cut(s) 70, 294, 454
MaeI CTAG 1 cut(s) 336
MaeII ACGT 1 cut(s) 600
MaeIII GTNAC 2 cut(s) 650, 683
MalI GATC 9 cut(s) 87, 133, 294, 323, 451, 641, 741, 826, 861
MboI GATC 9 cut(s) 85, 131, 292, 321, 449, 639, 739, 824, 859
MboII GAAGA 7 cut(s) 13, 177, 180, 191, 317, 478, 649
MflI RGATCY 3 cut(s) 131, 321, 449
MhlI GDGCHC 2 cut(s) 759, 980
MluCI AATT 6 cut(s) 213, 401, 471, 660, 717, 803
MlyI GAGTC 1 cut(s) 317
MmeI TCCRAC 2 cut(s) 133, 207
MnlI CCTC 7 cut(s) 76, 164, 388, 526, 590, 718, 833
MroNI GCCGGC 1 cut(s) 99
MseI TTAA 1 cut(s) 404
MslI CAYNNNNRTG 2 cut(s) 905, 1010
MspI CCGG 3 cut(s) 100, 252, 296
Mva1269I GAATGC 2 cut(s) 10, 938
MvnI CGCG 1 cut(s) 629
MwoI GCNNNNNNNGC 4 cut(s) 57, 80, 100, 473
NaeI GCCGGC 1 cut(s) 101
NcoI CCATGG 1 cut(s) 655
NdeII GATC 9 cut(s) 85, 131, 292, 321, 449, 639, 739, 824, 859
NgoMIV GCCGGC 1 cut(s) 99
NlaIII CATG 9 cut(s) 92, 377, 448, 542, 659, 683, 837, 904, 1009
NmuCI GTSAC 2 cut(s) 650, 683
PagI TCATGA 2 cut(s) 88, 373
PciSI GCTCTTC 1 cut(s) 204
PctI GAATGC 2 cut(s) 10, 938
PdiI GCCGGC 1 cut(s) 101
PfeI GAWTC 3 cut(s) 605, 620, 799
Pfl23II CGTACG 1 cut(s) 855
PflFI GACNNNGTC 1 cut(s) 539
PkrI GCNGC 5 cut(s) 53, 56, 59, 76, 105
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
PpuMI RGGWCCY 1 cut(s) 988
Psp5II RGGWCCY 1 cut(s) 988
PspEI GGTNACC 1 cut(s) 650
PspFI CCCAGC 1 cut(s) 328
PspLI CGTACG 1 cut(s) 855
PspPI GGNCC 3 cut(s) 397, 535, 988
PspPPI RGGWCCY 1 cut(s) 988
PstI CTGCAG 1 cut(s) 59
PsuI RGATCY 3 cut(s) 131, 321, 449
PsyI GACNNNGTC 1 cut(s) 539
RsaI GTAC 5 cut(s) 42, 356, 578, 784, 857
RsaNI GTAC 5 cut(s) 41, 355, 577, 783, 856
RseI CAYNNNNRTG 2 cut(s) 905, 1010
SapI GCTCTTC 1 cut(s) 204
SaqAI TTAA 1 cut(s) 404
SatI GCNGC 5 cut(s) 52, 55, 58, 75, 104
Sau3AI GATC 9 cut(s) 85, 131, 292, 321, 449, 639, 739, 824, 859
Sau96I GGNCC 3 cut(s) 397, 535, 988
ScaI AGTACT 1 cut(s) 784
SchI GAGTC 1 cut(s) 317
SduI GDGCHC 2 cut(s) 759, 980
SfaNI GCATC 3 cut(s) 70, 294, 454
SfcI CTRYAG 1 cut(s) 55
SgrAI CRCCGGYG 1 cut(s) 251
SinI GGWCC 2 cut(s) 535, 988
SmiMI CAYNNNNRTG 2 cut(s) 905, 1010
SmlI CTYRAG 2 cut(s) 593, 722
SmoI CTYRAG 2 cut(s) 593, 722
Sse9I AATT 6 cut(s) 213, 401, 471, 660, 717, 803
SsiI CCGC 1 cut(s) 117
SspI AATATT 1 cut(s) 589
SspMI CTAG 1 cut(s) 336
StyI CCWWGG 1 cut(s) 655
TaiI ACGT 1 cut(s) 603
TaqI TCGA 4 cut(s) 234, 288, 623, 827
TaqII GACCGA 1 cut(s) 638
TasI AATT 6 cut(s) 213, 401, 471, 660, 717, 803
TatI WGTACW 2 cut(s) 40, 782
TfiI GAWTC 3 cut(s) 605, 620, 799
Tru1I TTAA 1 cut(s) 404
Tru9I TTAA 1 cut(s) 404
TscAI CASTG 2 cut(s) 529, 912
TseFI GTSAC 2 cut(s) 650, 683
TseI GCWGC 5 cut(s) 51, 54, 57, 74, 103
Tsp45I GTSAC 2 cut(s) 650, 683
TspDTI ATGAA 3 cut(s) 17, 445, 889
TspGWI ACGGA 2 cut(s) 144, 903
TspRI CASTG 2 cut(s) 529, 912
Tth111I GACNNNGTC 1 cut(s) 539
VpaK11BI GGWCC 2 cut(s) 535, 988
XmiI GTMKAC 1 cut(s) 543
XspI CTAG 1 cut(s) 336
ZrmI AGTACT 1 cut(s) 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.