MD13G1025800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011996
Physical Location & Seq
Reverse (-)
1838576 .. 1840795
2220 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1025800.v1.1.491

Sequence Viewer

Length: 507 bp
ATGGCATTCTCTAGTTGCAGCAATCTGGTGGGCGGTCCGCTACCTTTGGCGTCGGTCGGAGAAACCCAGTTTCCGACGAAGCGCTCGATGTCGGTGACAACCACCACAAAGCACAGAGCTCTTCCTCTTCAGGTGAGAAGTGAGGGAAAACAAAAACCAGGCTCTCCAAAAACCTTCGGAGTTTCACGAAGAGATGTGATGTTATCTTTGCCAGTTGGGACATTGGCATCACTCACTCTTCTTCCAACAGAACCTGCCGAAGCACGCATTGTCAATCCCGAGATCAGGAAGAAAATTTTTGAAAAGTTAAAGAAGATAAGGGAACACTTTGGTTTGTCAAAGCCAAAAACCAATGACGGGAAAGAGGCATACCCATCGCCGCCGCCGCCGCCACCACCACCATCGTTGGAGGAGAAAAATCCTCAAACATTTCCACCAGCACTGCCCCTGGATCCTCAAGGGAACCTACTTGTACCTCTACCTCCTGTTGAAGTAATTCTCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

169

Amino Acids

18.16

Weight (kDa)

9.83

Isoelectric Point (pI)

67.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G01400 AT2G01400
fragaria_vesca FvH4_4g32380
malus_domestica MD13G1025800.v1.1 MD16G1028600.v1.1
prunus_persica Prupe.1G324100_v2.0.a1
pyrus_communis pycom13g02440 pycom16g02450
rosa_chinensis RchiOBHm_Chr4g0441211
rosa_laevigata RLG00000006111
rosa_multiflora Rmu_co7962724.1_g000001 Rmu_sc0006317.1_g000028
rosa_roxburghii Rroxscaffold_5G00382120
rosa_rugosa Rorug04G0331500
rosa_samantha Rh4AG383300 Rh4BG397400 Rh4CG412600 Rh4DG390100
rosa_wichuraiana Rw4G033070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 262
AciI CCGC 6 cut(s) 33, 38, 380, 383, 386, 389
AclWI GGATC 2 cut(s) 446, 459
AcsI RAATTY 1 cut(s) 294
AcuI CTGAAG 1 cut(s) 113
AcyI GRCGYC 1 cut(s) 50
AfaI GTAC 1 cut(s) 474
AfeI AGCGCT 1 cut(s) 83
AfiI CCNNNNNNNGG 2 cut(s) 285, 357
AgsI TTSAA 2 cut(s) 302, 491
AjnI CCWGG 2 cut(s) 157, 447
AluBI AGCT 1 cut(s) 119
AluI AGCT 1 cut(s) 119
Alw21I GWGCWC 1 cut(s) 121
AlwI GGATC 2 cut(s) 446, 459
AlwNI CAGNNNCTG 1 cut(s) 254
Ama87I CYCGRG 1 cut(s) 278
Aor51HI AGCGCT 1 cut(s) 83
ApeKI GCWGC 1 cut(s) 18
ApoI RAATTY 1 cut(s) 294
Asp700I GAANNNNTTC 1 cut(s) 495
AspLEI GCGC 1 cut(s) 84
AspS9I GGNCC 1 cut(s) 35
AsuHPI GGTGA 2 cut(s) 106, 145
AvaI CYCGRG 1 cut(s) 278
AvaII GGWCC 1 cut(s) 35
BamHI GGATCC 1 cut(s) 451
BanII GRGCYC 1 cut(s) 121
Bbv12I GWGCWC 1 cut(s) 121
BbvI GCAGC 1 cut(s) 30
BccI CCATC 2 cut(s) 382, 409
BcgI CGANNNNNNTGC 2 cut(s) 357, 391
BciT130I CCWGG 2 cut(s) 159, 449
BfaI CTAG 1 cut(s) 12
BfoI RGCGCY 1 cut(s) 85
BfuAI ACCTGC 1 cut(s) 262
BisI GCNGC 5 cut(s) 19, 380, 383, 386, 389
BlsI GCNGC 5 cut(s) 20, 381, 384, 387, 390
Bme1390I CCNGG 2 cut(s) 159, 449
Bme18I GGWCC 1 cut(s) 35
BmeT110I CYCGRG 1 cut(s) 278
BmgT120I GGNCC 1 cut(s) 35
BmiI GGNNCC 2 cut(s) 453, 464
BmrFI CCNGG 2 cut(s) 159, 449
BmrI ACTGGG 1 cut(s) 61
BmsI GCATC 1 cut(s) 236
BmuI ACTGGG 1 cut(s) 61
BpuEI CTTGAG 1 cut(s) 441
BsaHI GRCGYC 1 cut(s) 50
BsaJI CCNNGG 1 cut(s) 447
Bsc4I CCNNNNNNNGG 2 cut(s) 285, 357
Bse1I ACTGG 2 cut(s) 67, 212
BseBI CCWGG 2 cut(s) 159, 449
BseDI CCNNGG 1 cut(s) 447
BseLI CCNNNNNNNGG 2 cut(s) 285, 357
BseNI ACTGG 2 cut(s) 67, 212
BseRI GAGGAG 1 cut(s) 425
BseXI GCAGC 1 cut(s) 30
Bsh1285I CGRYCG 1 cut(s) 57
BsiEI CGRYCG 1 cut(s) 57
BsiHKAI GWGCWC 1 cut(s) 121
BsiHKCI CYCGRG 1 cut(s) 278
BslFI GGGAC 1 cut(s) 232
BslI CCNNNNNNNGG 2 cut(s) 285, 357
BsmFI GGGAC 1 cut(s) 232
BsmI GAATGC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 278
Bsp1286I GDGCHC 1 cut(s) 121
Bsp143I GATC 2 cut(s) 282, 451
BspACI CCGC 6 cut(s) 33, 38, 380, 383, 386, 389
BspLI GGNNCC 2 cut(s) 453, 464
BspMI ACCTGC 1 cut(s) 262
BspPI GGATC 2 cut(s) 446, 459
BspQI GCTCTTC 1 cut(s) 126
BsrI ACTGG 2 cut(s) 67, 212
BssECI CCNNGG 1 cut(s) 447
BssMI GATC 2 cut(s) 282, 451
BssNI GRCGYC 1 cut(s) 50
Bst2UI CCWGG 2 cut(s) 159, 449
Bst6I CTCTTC 4 cut(s) 126, 132, 184, 243
BstACI GRCGYC 1 cut(s) 50
BstC8I GCNNGC 1 cut(s) 265
BstH2I RGCGCY 1 cut(s) 85
BstHHI GCGC 1 cut(s) 84
BstKTI GATC 2 cut(s) 285, 454
BstMBI GATC 2 cut(s) 282, 451
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 2 cut(s) 385, 388
BstNI CCWGG 2 cut(s) 159, 449
BstSCI CCNGG 2 cut(s) 157, 447
BstV1I GCAGC 1 cut(s) 30
BstX2I RGATCY 1 cut(s) 451
BstYI RGATCY 1 cut(s) 451
BtgZI GCGATG 1 cut(s) 360
BtsI GCAGTG 1 cut(s) 440
BtsIMutI CAGTG 1 cut(s) 440
BveI ACCTGC 1 cut(s) 262
Cac8I GCNNGC 1 cut(s) 265
CaiI CAGNNNCTG 1 cut(s) 254
CfoI GCGC 1 cut(s) 84
Cfr13I GGNCC 1 cut(s) 35
CpoI CGGWCCG 1 cut(s) 35
CseI GACGC 1 cut(s) 39
Csp6I GTAC 1 cut(s) 473
CspCI CAANNNNNGTGG 2 cut(s) 387, 422
CspI CGGWCCG 1 cut(s) 35
CviJI RGCY 3 cut(s) 119, 162, 343
CviKI_1 RGCY 3 cut(s) 119, 162, 343
CviQI GTAC 1 cut(s) 473
DpnI GATC 2 cut(s) 284, 453
DpnII GATC 2 cut(s) 282, 451
Eam1104I CTCTTC 4 cut(s) 126, 132, 184, 243
EarI CTCTTC 4 cut(s) 126, 132, 184, 243
Ecl136II GAGCTC 1 cut(s) 119
Eco24I GRGCYC 1 cut(s) 121
Eco47I GGWCC 1 cut(s) 35
Eco47III AGCGCT 1 cut(s) 83
Eco53kI GAGCTC 1 cut(s) 119
Eco57I CTGAAG 1 cut(s) 113
Eco88I CYCGRG 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 119
EcoRII CCWGG 2 cut(s) 157, 447
EcoT38I GRGCYC 1 cut(s) 121
FaiI YATR 1 cut(s) 370
FaqI GGGAC 1 cut(s) 232
Fnu4HI GCNGC 5 cut(s) 19, 380, 383, 386, 389
FriOI GRGCYC 1 cut(s) 121
Fsp4HI GCNGC 5 cut(s) 19, 380, 383, 386, 389
FspBI CTAG 1 cut(s) 12
GlaI GCGC 1 cut(s) 83
GluI GCNGC 5 cut(s) 19, 380, 383, 386, 389
HaeII RGCGCY 1 cut(s) 85
HgaI GACGC 1 cut(s) 39
HhaI GCGC 1 cut(s) 84
Hin1I GRCGYC 1 cut(s) 50
Hin6I GCGC 1 cut(s) 82
HinP1I GCGC 1 cut(s) 82
HphI GGTGA 2 cut(s) 106, 145
Hpy188I TCNGA 3 cut(s) 59, 75, 179
Hpy188III TCNNGA 3 cut(s) 186, 278, 286
Hpy99I CGWCG 2 cut(s) 55, 79
HpyAV CCTTC 1 cut(s) 184
HpyCH4V TGCA 1 cut(s) 18
HpyF10VI GCNNNNNNNGC 2 cut(s) 385, 388
Hsp92I GRCGYC 1 cut(s) 50
HspAI GCGC 1 cut(s) 82
Kzo9I GATC 2 cut(s) 282, 451
LguI GCTCTTC 1 cut(s) 126
Lsp1109I GCAGC 1 cut(s) 30
LweI GCATC 1 cut(s) 236
MaeI CTAG 1 cut(s) 12
MaeIII GTNAC 1 cut(s) 94
MalI GATC 2 cut(s) 284, 453
MboI GATC 2 cut(s) 282, 451
MboII GAAGA 7 cut(s) 113, 119, 201, 230, 233, 301, 325
MflI RGATCY 1 cut(s) 451
MhlI GDGCHC 1 cut(s) 121
MluCI AATT 2 cut(s) 294, 495
MmeI TCCRAC 4 cut(s) 37, 98, 269, 387
MnlI CCTC 8 cut(s) 135, 136, 358, 403, 432, 465, 486, 492
MroXI GAANNNNTTC 1 cut(s) 495
MseI TTAA 1 cut(s) 308
MspR9I CCNGG 2 cut(s) 159, 449
Mva1269I GAATGC 1 cut(s) 5
MvaI CCWGG 2 cut(s) 159, 449
MwoI GCNNNNNNNGC 2 cut(s) 385, 388
NdeII GATC 2 cut(s) 282, 451
NlaIV GGNNCC 2 cut(s) 453, 464
NmuCI GTSAC 1 cut(s) 94
PciSI GCTCTTC 1 cut(s) 126
PcsI WCGNNNNNNNCGW 1 cut(s) 83
PctI GAATGC 1 cut(s) 5
PdmI GAANNNNTTC 1 cut(s) 495
PkrI GCNGC 5 cut(s) 20, 381, 384, 387, 390
Psp124BI GAGCTC 1 cut(s) 121
Psp6I CCWGG 2 cut(s) 157, 447
PspGI CCWGG 2 cut(s) 157, 447
PspN4I GGNNCC 2 cut(s) 453, 464
PspPI GGNCC 1 cut(s) 35
PstNI CAGNNNCTG 1 cut(s) 254
PsuI RGATCY 1 cut(s) 451
RsaI GTAC 1 cut(s) 474
RsaNI GTAC 1 cut(s) 473
Rsr2I CGGWCCG 1 cut(s) 35
RsrII CGGWCCG 1 cut(s) 35
SacI GAGCTC 1 cut(s) 121
SapI GCTCTTC 1 cut(s) 126
SaqAI TTAA 1 cut(s) 308
SatI GCNGC 5 cut(s) 19, 380, 383, 386, 389
Sau3AI GATC 2 cut(s) 282, 451
Sau96I GGNCC 1 cut(s) 35
ScrFI CCNGG 2 cut(s) 159, 449
SduI GDGCHC 1 cut(s) 121
SetI ASST 8 cut(s) 46, 121, 135, 176, 256, 468, 478, 484
SfaNI GCATC 1 cut(s) 236
SinI GGWCC 1 cut(s) 35
SmlI CTYRAG 1 cut(s) 456
SmoI CTYRAG 1 cut(s) 456
Sse9I AATT 2 cut(s) 294, 495
SsiI CCGC 6 cut(s) 33, 38, 380, 383, 386, 389
SspMI CTAG 1 cut(s) 12
SstI GAGCTC 1 cut(s) 121
StyD4I CCNGG 2 cut(s) 157, 447
TaqI TCGA 1 cut(s) 86
TaqII GACCGA 1 cut(s) 43
TasI AATT 2 cut(s) 294, 495
TauI GCSGC 4 cut(s) 382, 385, 388, 391
Tru1I TTAA 1 cut(s) 308
Tru9I TTAA 1 cut(s) 308
TscAI CASTG 1 cut(s) 447
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 1 cut(s) 18
Tsp45I GTSAC 1 cut(s) 94
TspRI CASTG 1 cut(s) 447
VpaK11BI GGWCC 1 cut(s) 35
XapI RAATTY 1 cut(s) 294
XmnI GAANNNNTTC 1 cut(s) 495
XspI CTAG 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.