Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0012227

Physical Location & Seq

Genomic Coordinates Reverse (-)

1897216 .. 1898777

Gene Structure 1562 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1026300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1137 bp

ATGCGTCGACTTCTTCCACGAATTCCAGTCCGACAGAATCCTGGGTATCTGAACTGGTGGTCGCCCTCCAGGCCGCCAAGCGCCTCGTTTTGCTTGCCGCCAACTTCACTTCCAATTTGGCCCCTCCCGGTCTCCACCAATTACAATGACGAAGCGAGCAAGAAGATAAGCTTCCAATTCCAATGCGTGACGTGGAAATTGGAGAAGGCGCTGGGCGACATACGGTACGACCAGTTCGACATCTCCGAGGAAGTTGAAGAACAGGTTGAATTGGTTAGAGCCCAGTTGAGAAGAGCAATAGAAAGATATGGGTCTTCCATGAAAGAGAGAACGAACAATGCAACGACGATTGGCAGTATTCCCAAAAGCTTTAGTGCTGATTCTGCTGATGACATTGTGATTCGTAAATCCGAAACCCTTCGTGCTGCTGACTCTGCTGATGACATCCATGTTGATGATCGTGGCGATATCAAGAAGCCTGATGCCAACTCATCTGCAATTCCTGACGATTTTATATGCCCAATATCTTTGGAACTCATGAGGGATCCTGTTATTGTGGCCACAGGTCAGACGTACGAGAGATCTTACATACAGAGATGGGTAGATTGTGGGAACACAACATGTCCGAAAACTCAGCAAAAGTTGGTGAATTTGACACTAACTCCGAACTATGTTTTGAGGAGTTTGATTGCACAGTGGTGTATTAACAGCAATATAGAGCAGCTCAACGGGAAGATCAAGAAGAGTGATGGATCATTCCGTAACATTACTGGGGACACAGCGACGATTCAAGCCTTGGTTCGCAAGCTCTCCAGTCGATCAATTGAGGAGCGCAGAGCTGCTGCAGCTGAGATTCGATCTCTTTCCAAAAGAAGCACTGACAACAGAATACTAATTGCGGAAGCGGGTGCCATACCTGTTATAGTCAACCTATTAACATCAGAAGATGGACTAAGACAAGAGAATGCAGTAACTTCCGTTCTGAAACTCTCAATATATGACAATAACAATGAGCTCATTATGCTTGCCGGTGCTGTTCCTTCCATTGTCCGAGTCCTCAGACCCGGAAGCATGGAAACGAAAGAGAATGCAGCAGCAACCTTTTTAGTTTGTCACTTGCTGATGAGAACAAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009966 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010646 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0023051 GO:0031647 GO:0031648 GO:0032446 GO:0036211 GO:0042221 GO:0042493 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051865 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901698 GO:1901700 GO:2000022

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Arm U-box

Protein Analysis

379

Amino Acids

42.31

Weight (kDa)

8.61

Isoelectric Point (pI)

43.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUB10_N	PF25368	47 - 99	3.5e-23	PUB10-like, N-terminal domain
U-box	PF04564	167 - 234	6.9e-20	U-box domain
ARM_PUB	PF25598	263 - 366	8.4e-07	Plant U-box protein ARM repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012227)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23030 AT1G71020 AT1G71020
fragaria_vesca	FvH4_4g32300
malus_domestica	MD13G1026300.v1.1 MD16G1029300.v1.1
prunus_persica	Prupe.1G323500_v2.0.a1
pyrus_communis	pycom16g02520
rosa_chinensis	RchiOBHm_Chr4g0441091
rosa_laevigata	RLG00000006115
rosa_multiflora	Rmu_co8350821.1_g000001 Rmu_sc0006317.1_g000015
rosa_roxburghii	Rroxscaffold_5G00382050
rosa_rugosa	Rorug04G0331000
rosa_samantha	Rh4AG382600 Rh4BG396400 Rh4CG411500 Rh4DG389200
rosa_wichuraiana	Rw4G033020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	908
AccI	GTMKAC	1 cut(s)	7
AciI	CCGC	4 cut(s)	74, 98, 899, 905
AclWI	GGATC	3 cut(s)	539, 552, 760
AcoI	YGGCCR	1 cut(s)	558
AcsI	RAATTY	2 cut(s)	21, 649
AfaI	GTAC	2 cut(s)	227, 575
AflIII	ACRYGT	1 cut(s)	620
AgsI	TTSAA	3 cut(s)	257, 269, 791
AjiI	CACGTC	1 cut(s)	192
AjnI	CCWGG	2 cut(s)	40, 68
AleI	CACNNNNGTG	1 cut(s)	697
AluBI	AGCT	7 cut(s)	171, 369, 724, 808, 839, 848, 1015
AluI	AGCT	7 cut(s)	171, 369, 724, 808, 839, 848, 1015
Alw21I	GWGCWC	1 cut(s)	1017
Alw26I	GTCTC	1 cut(s)	136
AlwI	GGATC	3 cut(s)	539, 552, 760
AoxI	GGCC	3 cut(s)	71, 119, 558
ApeKI	GCWGC	7 cut(s)	425, 721, 839, 842, 845, 1091, 1094
ApoI	RAATTY	2 cut(s)	21, 649
Asp700I	GAANNNNTTC	1 cut(s)	417
AspLEI	GCGC	3 cut(s)	83, 211, 834
AspS9I	GGNCC	1 cut(s)	120
AsuC2I	CCSGG	2 cut(s)	128, 1065
AsuHPI	GGTGA	1 cut(s)	658
BalI	TGGCCA	1 cut(s)	560
BamHI	GGATCC	1 cut(s)	544
BanI	GGYRCC	1 cut(s)	908
BanII	GRGCYC	2 cut(s)	283, 1017
BbsI	GAAGAC	1 cut(s)	306
Bbv12I	GWGCWC	1 cut(s)	1017
BbvI	GCAGC	7 cut(s)	412, 733, 826, 829, 857, 1103, 1106
BccI	CCATC	3 cut(s)	591, 743, 941
BciT130I	CCWGG	2 cut(s)	42, 70
BcnI	CCSGG	2 cut(s)	128, 1065
BcoDI	GTCTC	1 cut(s)	136
BfmI	CTRYAG	1 cut(s)	843
BfoI	RGCGCY	2 cut(s)	84, 212
BglI	GCCNNNNNGGC	1 cut(s)	70
BglII	AGATCT	1 cut(s)	581
BisI	GCNGC	9 cut(s)	74, 98, 426, 722, 840, 843, 846, 1092, 1095
BlsI	GCNGC	9 cut(s)	75, 99, 427, 723, 841, 844, 847, 1093, 1096
Bme1390I	CCNGG	4 cut(s)	42, 70, 128, 1065
BmgBI	CACGTC	1 cut(s)	192
BmgT120I	GGNCC	1 cut(s)	120
BmiI	GGNNCC	3 cut(s)	122, 546, 910
BmrFI	CCNGG	4 cut(s)	42, 70, 128, 1065
BmrI	ACTGGG	2 cut(s)	277, 780
BmsI	GCATC	1 cut(s)	472
BmuI	ACTGGG	2 cut(s)	277, 780
BpiI	GAAGAC	1 cut(s)	306
BpmI	CTGGAG	2 cut(s)	52, 796
BpuMI	CCSGG	2 cut(s)	128, 1065
BsaI	GGTCTC	1 cut(s)	136
BsaJI	CCNNGG	3 cut(s)	41, 246, 795
BsaXI	ACNNNNNCTCC	2 cut(s)	646, 676
Bse118I	RCCGGY	1 cut(s)	1028
Bse1I	ACTGG	6 cut(s)	26, 59, 232, 283, 775, 813
BseBI	CCWGG	2 cut(s)	42, 70
BseDI	CCNNGG	3 cut(s)	41, 246, 795
BseGI	GGATG	1 cut(s)	444
BseMII	CTCAG	3 cut(s)	647, 840, 1072
BseNI	ACTGG	6 cut(s)	26, 59, 232, 283, 775, 813
BseRI	GAGGAG	2 cut(s)	694, 842
BseXI	GCAGC	7 cut(s)	412, 733, 826, 829, 857, 1103, 1106
BseYI	CCCAGC	1 cut(s)	211
BshFI	GGCC	3 cut(s)	73, 121, 560
BshNI	GGYRCC	1 cut(s)	908
BsiHKAI	GWGCWC	1 cut(s)	1017
BsiSI	CCGG	3 cut(s)	128, 1029, 1065
BsiWI	CGTACG	1 cut(s)	573
BslFI	GGGAC	1 cut(s)	788
BsmAI	GTCTC	1 cut(s)	136
BsmFI	GGGAC	1 cut(s)	788
BsmI	GAATGC	2 cut(s)	970, 1093
BsnI	GGCC	3 cut(s)	73, 121, 560
Bso31I	GGTCTC	1 cut(s)	136
Bsp1286I	GDGCHC	2 cut(s)	283, 1017
Bsp143I	GATC	7 cut(s)	457, 544, 581, 735, 752, 818, 857
BspACI	CCGC	4 cut(s)	74, 98, 899, 905
BspANI	GGCC	3 cut(s)	73, 121, 560
BspCNI	CTCAG	3 cut(s)	646, 841, 1071
BspHI	TCATGA	1 cut(s)	537
BspLI	GGNNCC	3 cut(s)	122, 546, 910
BspMAI	CTGCAG	1 cut(s)	847
BspPI	GGATC	3 cut(s)	539, 552, 760
BspQI	GCTCTTC	1 cut(s)	286
BspT107I	GGYRCC	1 cut(s)	908
BspTNI	GGTCTC	1 cut(s)	136
BsrFI	RCCGGY	1 cut(s)	1028
BsrI	ACTGG	6 cut(s)	26, 59, 232, 283, 775, 813
BssAI	RCCGGY	1 cut(s)	1028
BssECI	CCNNGG	3 cut(s)	41, 246, 795
BssMI	GATC	7 cut(s)	457, 544, 581, 735, 752, 818, 857
BssT1I	CCWWGG	1 cut(s)	795
Bst2UI	CCWGG	2 cut(s)	42, 70
Bst4CI	ACNGT	2 cut(s)	225, 696
Bst6I	CTCTTC	2 cut(s)	286, 737
BstC8I	GCNNGC	4 cut(s)	95, 157, 806, 1026
BstDEI	CTNAG	4 cut(s)	633, 849, 953, 1058
BstF5I	GGATG	1 cut(s)	444
BstH2I	RGCGCY	2 cut(s)	84, 212
BstHHI	GCGC	3 cut(s)	83, 211, 834
BstKTI	GATC	7 cut(s)	460, 547, 584, 738, 755, 821, 860
BstMAI	GTCTC	1 cut(s)	136
BstMBI	GATC	7 cut(s)	457, 544, 581, 735, 752, 818, 857
BstMWI	GCNNNNNNNGC	5 cut(s)	70, 383, 434, 845, 1021
BstNI	CCWGG	2 cut(s)	42, 70
BstNSI	RCATGY	1 cut(s)	624
BstSCI	CCNGG	4 cut(s)	40, 68, 126, 1063
BstSFI	CTRYAG	1 cut(s)	843
BstV1I	GCAGC	7 cut(s)	412, 733, 826, 829, 857, 1103, 1106
BstV2I	GAAGAC	1 cut(s)	306
BstX2I	RGATCY	2 cut(s)	544, 581
BstYI	RGATCY	2 cut(s)	544, 581
BsuRI	GGCC	3 cut(s)	73, 121, 560
BtrI	CACGTC	1 cut(s)	192
BtsCI	GGATG	1 cut(s)	444
BtsIMutI	CAGTG	2 cut(s)	701, 876
Cac8I	GCNNGC	4 cut(s)	95, 157, 806, 1026
CciI	TCATGA	1 cut(s)	537
CfoI	GCGC	3 cut(s)	83, 211, 834
Cfr10I	RCCGGY	1 cut(s)	1028
Cfr13I	GGNCC	1 cut(s)	120
Csp6I	GTAC	2 cut(s)	226, 574
CviAII	CATG	5 cut(s)	319, 449, 538, 621, 1072
CviQI	GTAC	2 cut(s)	226, 574
DdeI	CTNAG	4 cut(s)	633, 849, 953, 1058
DpnI	GATC	7 cut(s)	459, 546, 583, 737, 754, 820, 859
DpnII	GATC	7 cut(s)	457, 544, 581, 735, 752, 818, 857
EaeI	YGGCCR	1 cut(s)	558
Eam1104I	CTCTTC	2 cut(s)	286, 737
EarI	CTCTTC	2 cut(s)	286, 737
Ecl136II	GAGCTC	1 cut(s)	1015
Eco130I	CCWWGG	1 cut(s)	795
Eco24I	GRGCYC	2 cut(s)	283, 1017
Eco31I	GGTCTC	1 cut(s)	136
Eco32I	GATATC	1 cut(s)	469
Eco53kI	GAGCTC	1 cut(s)	1015
EcoICRI	GAGCTC	1 cut(s)	1015
EcoRI	GAATTC	1 cut(s)	21
EcoRII	CCWGG	2 cut(s)	40, 68
EcoRV	GATATC	1 cut(s)	469
EcoT14I	CCWWGG	1 cut(s)	795
EcoT38I	GRGCYC	2 cut(s)	283, 1017
ErhI	CCWWGG	1 cut(s)	795
FaeI	CATG	5 cut(s)	322, 452, 541, 624, 1075
FalI	AAGNNNNNCTT	2 cut(s)	155, 187
FaqI	GGGAC	1 cut(s)	788
FatI	CATG	5 cut(s)	318, 448, 537, 620, 1071
FauI	CCCGC	1 cut(s)	898
FblI	GTMKAC	1 cut(s)	7
Fnu4HI	GCNGC	9 cut(s)	74, 98, 426, 722, 840, 843, 846, 1092, 1095
FokI	GGATG	1 cut(s)	431
FriOI	GRGCYC	2 cut(s)	283, 1017
Fsp4HI	GCNGC	9 cut(s)	74, 98, 426, 722, 840, 843, 846, 1092, 1095
GlaI	GCGC	3 cut(s)	82, 210, 833
GluI	GCNGC	9 cut(s)	74, 98, 426, 722, 840, 843, 846, 1092, 1095
GsaI	CCCAGC	1 cut(s)	215
GsuI	CTGGAG	2 cut(s)	52, 796
HaeII	RGCGCY	2 cut(s)	84, 212
HaeIII	GGCC	3 cut(s)	73, 121, 560
HapII	CCGG	3 cut(s)	128, 1029, 1065
HhaI	GCGC	3 cut(s)	83, 211, 834
Hin1II	CATG	5 cut(s)	322, 452, 541, 624, 1075
Hin6I	GCGC	3 cut(s)	81, 209, 832
HinP1I	GCGC	3 cut(s)	81, 209, 832
HincII	GTYRAC	2 cut(s)	8, 928
HindII	GTYRAC	2 cut(s)	8, 928
HindIII	AAGCTT	2 cut(s)	169, 367
HinfI	GANTC	7 cut(s)	37, 380, 400, 431, 787, 853, 1053
HpaII	CCGG	3 cut(s)	128, 1029, 1065
HphI	GGTGA	1 cut(s)	658
Hpy166II	GTNNAC	2 cut(s)	8, 928
Hpy188III	TCNNGA	4 cut(s)	472, 503, 538, 739
Hpy8I	GTNNAC	2 cut(s)	8, 928
Hpy99I	CGWCG	3 cut(s)	9, 349, 787
HpyAV	CCTTC	3 cut(s)	199, 428, 1050
HpyCH4III	ACNGT	2 cut(s)	225, 696
HpyCH4IV	ACGT	2 cut(s)	191, 572
HpyCH4V	TGCA	6 cut(s)	341, 497, 692, 845, 968, 1091
HpyF10VI	GCNNNNNNNGC	5 cut(s)	70, 383, 434, 845, 1021
HpyF3I	CTNAG	4 cut(s)	633, 849, 953, 1058
HpySE526I	ACGT	2 cut(s)	191, 572
Hsp92II	CATG	5 cut(s)	322, 452, 541, 624, 1075
HspAI	GCGC	3 cut(s)	81, 209, 832
Kzo9I	GATC	7 cut(s)	457, 544, 581, 735, 752, 818, 857
LguI	GCTCTTC	1 cut(s)	286
LmnI	GCTCC	1 cut(s)	829
Lsp1109I	GCAGC	7 cut(s)	412, 733, 826, 829, 857, 1103, 1106
LweI	GCATC	1 cut(s)	472
MaeII	ACGT	2 cut(s)	191, 572
MaeIII	GTNAC	4 cut(s)	187, 761, 970, 1112
MalI	GATC	7 cut(s)	459, 546, 583, 737, 754, 820, 859
MboI	GATC	7 cut(s)	457, 544, 581, 735, 752, 818, 857
MboII	GAAGA	8 cut(s)	5, 175, 269, 303, 306, 745, 754, 956
MfeI	CAATTG	1 cut(s)	822
MflI	RGATCY	2 cut(s)	544, 581
MhlI	GDGCHC	2 cut(s)	283, 1017
MlsI	TGGCCA	1 cut(s)	560
MluNI	TGGCCA	1 cut(s)	560
MlyI	GAGTC	2 cut(s)	425, 1062
MmeI	TCCRAC	1 cut(s)	55
MnlI	CCTC	8 cut(s)	76, 94, 134, 241, 534, 672, 820, 1067
Mox20I	TGGCCA	1 cut(s)	560
MroXI	GAANNNNTTC	1 cut(s)	417
MscI	TGGCCA	1 cut(s)	560
MseI	TTAA	2 cut(s)	705, 935
MslI	CAYNNNNRTG	2 cut(s)	453, 697
Msp20I	TGGCCA	1 cut(s)	560
MspA1I	CMGCKG	1 cut(s)	848
MspI	CCGG	3 cut(s)	128, 1029, 1065
MspR9I	CCNGG	4 cut(s)	42, 70, 128, 1065
MunI	CAATTG	1 cut(s)	822
Mva1269I	GAATGC	2 cut(s)	970, 1093
MvaI	CCWGG	2 cut(s)	42, 70
MwoI	GCNNNNNNNGC	5 cut(s)	70, 383, 434, 845, 1021
NciI	CCSGG	2 cut(s)	128, 1065
NdeII	GATC	7 cut(s)	457, 544, 581, 735, 752, 818, 857
NlaIII	CATG	5 cut(s)	322, 452, 541, 624, 1075
NlaIV	GGNNCC	3 cut(s)	122, 546, 910
NmuCI	GTSAC	2 cut(s)	187, 1112
NspI	RCATGY	1 cut(s)	624
OliI	CACNNNNGTG	1 cut(s)	697
PagI	TCATGA	1 cut(s)	537
PciI	ACATGT	1 cut(s)	620
PciSI	GCTCTTC	1 cut(s)	286
PcsI	WCGNNNNNNNCGW	2 cut(s)	234, 243
PctI	GAATGC	2 cut(s)	970, 1093
PdmI	GAANNNNTTC	1 cut(s)	417
PfeI	GAWTC	5 cut(s)	37, 380, 400, 787, 853
Pfl23II	CGTACG	1 cut(s)	573
PkrI	GCNGC	9 cut(s)	75, 99, 427, 723, 841, 844, 847, 1093, 1096
PleI	GAGTC	2 cut(s)	425, 1061
PpsI	GAGTC	2 cut(s)	425, 1061
PscI	ACATGT	1 cut(s)	620
Psp124BI	GAGCTC	1 cut(s)	1017
Psp6I	CCWGG	2 cut(s)	40, 68
PspFI	CCCAGC	1 cut(s)	211
PspGI	CCWGG	2 cut(s)	40, 68
PspLI	CGTACG	1 cut(s)	573
PspN4I	GGNNCC	3 cut(s)	122, 546, 910
PspPI	GGNCC	1 cut(s)	120
PsrI	GAACNNNNNNTAC	4 cut(s)	218, 250, 963, 995
PstI	CTGCAG	1 cut(s)	847
PsuI	RGATCY	2 cut(s)	544, 581
PvuII	CAGCTG	1 cut(s)	848
RsaI	GTAC	2 cut(s)	227, 575
RsaNI	GTAC	2 cut(s)	226, 574
RseI	CAYNNNNRTG	2 cut(s)	453, 697
SacI	GAGCTC	1 cut(s)	1017
SalI	GTCGAC	1 cut(s)	6
SapI	GCTCTTC	1 cut(s)	286
SaqAI	TTAA	2 cut(s)	705, 935
SatI	GCNGC	9 cut(s)	74, 98, 426, 722, 840, 843, 846, 1092, 1095
Sau3AI	GATC	7 cut(s)	457, 544, 581, 735, 752, 818, 857
Sau96I	GGNCC	1 cut(s)	120
SchI	GAGTC	2 cut(s)	425, 1062
ScrFI	CCNGG	4 cut(s)	42, 70, 128, 1065
SduI	GDGCHC	2 cut(s)	283, 1017
SfaNI	GCATC	1 cut(s)	472
SfcI	CTRYAG	1 cut(s)	843
SmiMI	CAYNNNNRTG	2 cut(s)	453, 697
SsiI	CCGC	4 cut(s)	74, 98, 899, 905
SstI	GAGCTC	1 cut(s)	1017
StyD4I	CCNGG	4 cut(s)	40, 68, 126, 1063
StyI	CCWWGG	1 cut(s)	795
TaaI	ACNGT	2 cut(s)	225, 696
TaiI	ACGT	2 cut(s)	194, 575
TaqI	TCGA	4 cut(s)	7, 237, 817, 856
TauI	GCSGC	2 cut(s)	76, 100
TfiI	GAWTC	5 cut(s)	37, 380, 400, 787, 853
Tru1I	TTAA	2 cut(s)	705, 935
Tru9I	TTAA	2 cut(s)	705, 935
TscAI	CASTG	2 cut(s)	701, 883
TseFI	GTSAC	2 cut(s)	187, 1112
TseI	GCWGC	7 cut(s)	425, 721, 839, 842, 845, 1091, 1094
Tsp45I	GTSAC	2 cut(s)	187, 1112
TspDTI	ATGAA	1 cut(s)	335
TspGWI	ACGGA	2 cut(s)	749, 967
TspRI	CASTG	2 cut(s)	701, 883
XapI	RAATTY	2 cut(s)	21, 649
XceI	RCATGY	1 cut(s)	624
XmiI	GTMKAC	1 cut(s)	7
XmnI	GAANNNNTTC	1 cut(s)	417

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.