Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0012227

Physical Location & Seq

Genomic Coordinates Forward (+)

61920818 .. 61923522

Gene Structure 2705 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00382050.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1896 bp

ATGGCAGGCGAAGCTGCCGGCGGCGCGTCGGAGCTTCTGCAACTCGTCAACGACGTCGTCGGCGCTGGAAAGTCCGCCAGCGGAGGAGGTGGCGGCGCCTTCAGGAAGGACTGCACCGATCTGGTGCGGCGGATCGCGCTGCTGACGCATTTGTTCGAGGAGATTAGGAACTTCAAAGGACTCGAATTTCCACCCTTGGATGCTTCCACTTCTTCTTCCTGCTCCACACAGCCGTGGGTTTCCGATCTCGTCACGGCGCTTCAGGCCGCGAAGCGGCTTGTATTGGTGGCCACTAGCTTCAATTCCAATTGTGACGCTCCTGTGCTCGCCGATGAAGCTAGCAAGAGGATAAGCTTCCATTTTCAATGTGCGACGTGGAAATTGGAGAAGGCGTTGGGTGAAATACCGTATGATCAGTTCGAAATATCAGAGGAAGTTGAAGAACAGGTTGCATTGGTTAGAGCACAATTGAGAAGAGCCATAGAAAGATATGGGACCTTGAATTTGAGGAACATGTCTTTTTATGGTCTACCTCAGTCTCTGGTAGAGTCTGGAAAATGGGTGCATGTTATAGAGAATAATAGTAGTTCAAAGATGACAGACAGCCTTCCCAAGAGCTTTAGTGCTGATGATCTGGTTCATAAATCGCAAAGGATGAGCAACTCCTGTGTGGCAGAGGTGCCTTCACTCTGCTCAACAAATGAGACTCATGTTGATGGTGAGGAAGATATGAACAAGAAGCCTGACGCCACTGTCATCCCTGATTTTATATGCCCAATATCTTTGGAACTCATGAGAGATCCTGTTATTGTGGCCACTGGTCAGACATACGAGAGATCTTACATACAGAGATGGATAGACTGCGGAAATACAACATGTCCAAAAACTCAGCAAAAGCTTCAAAATTTGACACTGACTCCAAATTATGTCTTGAGAAGTCTAGTTGCTCAGTGGTGTATTGACAACAACATCGAGCAGCCAACAGGATTAACAAATGGTAAACTCAAGAAGAGTGATGGATCCTTTCGTGACATTAGTGGGGACATAGCGACAATTCGAGCTCTGGTAAGCAAGCTTGCAGGTCGATCAATTGAGGAGCGGAGAGCAGCTGCAGCGGAAATTAGGTCACTGTCCAAAAGAAGTACTGATAACCGAATACTAATTGCAGAAGCGGGTGCTATTCCGGTATTGGTCAATCTCTTAACAGTAGAAGATGGCTTAACACAAGAGAATGCAGTGACTTCCATTCTCAACCTCTCTATATATGAAAACAACAAGGGGCTTATAATGCTGGCGGGTGCTGTTCCTTCTATTGTCCAAGTCCTCAGAGCTGGAAGCATGGAGGCTCGAGAGAACGCAGCAGCGACCATTTTTAGTTTGTCACTTGCCGATGAGAACAAAATAATTATAGGTGCATCGGGAGCAATTTCAGCTTTGGTGGAATTGCTTCAAAATGGGAGCACAAGAGGGAAGAAGGATGCTGCAACAGCGCTGTTCAATTTGTGCATTTATCAGGGCAACAAGGGCAGAGCTATCAGAGCAGGGATAATTACAGCTTTACTAAAGATGCTAACTGATTCAAGCAATTGCATGGTTGATGAAGCACTGACCATTATGTCAGTTCTTGCTAACCATCAAGAGGCAAAAATTACTATAGTAAAGGCTGGCACAATCCCTGTTTTGATAGACCTTTTGCGGACAGGCCTGCCTCGAAACAAAGAGAATGCATCTGCCATTTTGCTTGCATTGTGCAAGAGAGAGACTGAGAACCTTGCCTGCTTAAGTAGGCTTGGTGCAGTCATACCCTTGACAGAGCTCACTAAAAGCGGAACAGAGAGGGCCAAAAGGAAGGCTAGTTCATTGCTGGAGCATCTTCGGAAGTTGCAGCAGCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009966 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010646 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0023051 GO:0031647 GO:0031648 GO:0032446 GO:0036211 GO:0042221 GO:0042493 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051865 GO:0065007 GO:0065008 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901698 GO:1901700 GO:2000022

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Arm U-box

Protein Analysis

631

Amino Acids

68.34

Weight (kDa)

7.03

Isoelectric Point (pI)

45.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUB10_N	PF25368	35 - 160	2.3e-41	PUB10-like, N-terminal domain
U-box	PF04564	254 - 322	4.6e-18	U-box domain
ARM_PUB	PF25598	352 - 625	2.2e-23	Plant U-box protein ARM repeat region
ARM_ARMC5	PF24768	363 - 532	2e-09	ARMC5
Arm	PF00514	381 - 420	7.1e-08	Armadillo/beta-catenin-like repeat
Arm	PF00514	464 - 502	4.7e-08	Armadillo/beta-catenin-like repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012227)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23030 AT1G71020 AT1G71020
fragaria_vesca	FvH4_4g32300
malus_domestica	MD13G1026300.v1.1 MD16G1029300.v1.1
prunus_persica	Prupe.1G323500_v2.0.a1
pyrus_communis	pycom16g02520
rosa_chinensis	RchiOBHm_Chr4g0441091
rosa_laevigata	RLG00000006115
rosa_multiflora	Rmu_co8350821.1_g000001 Rmu_sc0006317.1_g000015
rosa_roxburghii	Rroxscaffold_5G00382050
rosa_rugosa	Rorug04G0331000
rosa_samantha	Rh4AG382600 Rh4BG396400 Rh4CG411500 Rh4DG389200
rosa_wichuraiana	Rw4G033020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1286
AasI	GACNNNNNNGTC	2 cut(s)	752, 1615
AatII	GACGTC	1 cut(s)	57
Acc36I	ACCTGC	1 cut(s)	1070
AccB1I	GGYRCC	2 cut(s)	95, 679
AccBSI	CCGCTC	1 cut(s)	1099
AccI	GTMKAC	1 cut(s)	529
AccII	CGCG	3 cut(s)	26, 137, 269
AclWI	GGATC	4 cut(s)	140, 794, 1014, 1027
AcoI	YGGCCR	2 cut(s)	288, 813
AcsI	RAATTY	3 cut(s)	185, 502, 904
AcuI	CTGAAG	2 cut(s)	85, 245
AcyI	GRCGYC	3 cut(s)	54, 96, 747
AfaI	GTAC	1 cut(s)	1144
AfeI	AGCGCT	1 cut(s)	1491
AfiI	CCNNNNNNNGG	2 cut(s)	273, 1639
AflII	CTTAAG	1 cut(s)	1780
AflIII	ACRYGT	2 cut(s)	513, 875
AjiI	CACGTC	1 cut(s)	375
AleI	CACNNNNGTG	1 cut(s)	232
AloI	GAACNNNNNNTCC	2 cut(s)	1840, 1872
Alw21I	GWGCWC	5 cut(s)	327, 466, 1063, 1463, 1818
Alw26I	GTCTC	3 cut(s)	543, 698, 1754
AlwI	GGATC	4 cut(s)	140, 794, 1014, 1027
AlwNI	CAGNNNCTG	1 cut(s)	541
Ama87I	CYCGRG	1 cut(s)	1347
Aor51HI	AGCGCT	1 cut(s)	1491
AoxI	GGCC	5 cut(s)	264, 288, 813, 1702, 1839
ApoI	RAATTY	3 cut(s)	185, 502, 904
ArsI	GACNNNNNNTTYG	2 cut(s)	487, 519
Asp700I	GAANNNNTTC	1 cut(s)	1446
AspLEI	GCGC	6 cut(s)	26, 65, 98, 139, 259, 1492
AspS9I	GGNCC	2 cut(s)	495, 1839
AsuHPI	GGTGA	2 cut(s)	410, 731
AsuII	TTCGAA	1 cut(s)	420
AsuNHI	GCTAGC	1 cut(s)	338
AvaI	CYCGRG	1 cut(s)	1347
AvaII	GGWCC	1 cut(s)	495
BalI	TGGCCA	2 cut(s)	290, 815
BamHI	GGATCC	1 cut(s)	1019
BanI	GGYRCC	2 cut(s)	95, 679
BanII	GRGCYC	2 cut(s)	1063, 1818
Bbv12I	GWGCWC	5 cut(s)	327, 466, 1063, 1463, 1818
BbvI	GCAGC	8 cut(s)	126, 988, 1096, 1118, 1124, 1370, 1373, 1468
BccI	CCATC	5 cut(s)	710, 846, 1010, 1208, 1641
BceAI	ACGGC	2 cut(s)	217, 270
BclI	TGATCA	1 cut(s)	412
BcoDI	GTCTC	3 cut(s)	543, 698, 1754
BfaI	CTAG	4 cut(s)	294, 339, 941, 1854
BfmI	CTRYAG	2 cut(s)	1110, 1653
BfoI	RGCGCY	4 cut(s)	66, 99, 260, 1493
BfrI	CTTAAG	1 cut(s)	1780
BfuAI	ACCTGC	1 cut(s)	1070
BglII	AGATCT	1 cut(s)	836
BmcAI	AGTACT	1 cut(s)	1144
Bme18I	GGWCC	1 cut(s)	495
BmeT110I	CYCGRG	1 cut(s)	1347
BmgBI	CACGTC	1 cut(s)	375
BmgT120I	GGNCC	2 cut(s)	495, 1839
BmiI	GGNNCC	4 cut(s)	97, 496, 681, 1021
BmsI	GCATC	6 cut(s)	190, 1424, 1468, 1557, 1736, 1879
BmtI	GCTAGC	1 cut(s)	342
BpmI	CTGGAG	1 cut(s)	1886
Bpu14I	TTCGAA	1 cut(s)	420
BpuEI	CTTGAG	2 cut(s)	952, 989
BsaHI	GRCGYC	3 cut(s)	54, 96, 747
BsaJI	CCNNGG	2 cut(s)	195, 233
BsaWI	WCCGGW	1 cut(s)	1183
BsaXI	ACNNNNNCTCC	4 cut(s)	377, 407, 901, 931
Bsc4I	CCNNNNNNNGG	2 cut(s)	273, 1639
Bse118I	RCCGGY	1 cut(s)	17
Bse1I	ACTGG	1 cut(s)	823
Bse3DI	GCAATG	1 cut(s)	1859
BseDI	CCNNGG	2 cut(s)	195, 233
BseGI	GGATG	4 cut(s)	205, 660, 756, 1483
BseLI	CCNNNNNNNGG	2 cut(s)	273, 1639
BseMI	GCAATG	1 cut(s)	1859
BseMII	CTCAG	5 cut(s)	548, 902, 962, 1339, 1755
BseNI	ACTGG	1 cut(s)	823
BseRI	GAGGAG	3 cut(s)	99, 173, 1109
BseXI	GCAGC	8 cut(s)	126, 988, 1096, 1118, 1124, 1370, 1373, 1468
BsgI	GTGCAG	2 cut(s)	97, 1815
Bsh1236I	CGCG	3 cut(s)	26, 137, 269
BshFI	GGCC	5 cut(s)	266, 290, 815, 1704, 1841
BshNI	GGYRCC	2 cut(s)	95, 679
BsiHKAI	GWGCWC	5 cut(s)	327, 466, 1063, 1463, 1818
BsiHKCI	CYCGRG	1 cut(s)	1347
BsiSI	CCGG	2 cut(s)	18, 1184
BslFI	GGGAC	2 cut(s)	508, 1055
BslI	CCNNNNNNNGG	2 cut(s)	273, 1639
BsmAI	GTCTC	3 cut(s)	543, 698, 1754
BsmFI	GGGAC	2 cut(s)	508, 1055
BsmI	GAATGC	2 cut(s)	1237, 1729
BsnI	GGCC	5 cut(s)	266, 290, 815, 1704, 1841
BsoBI	CYCGRG	1 cut(s)	1347
Bsp119I	TTCGAA	1 cut(s)	420
Bsp1286I	GDGCHC	5 cut(s)	327, 466, 1063, 1463, 1818
Bsp143I	GATC	9 cut(s)	118, 132, 244, 412, 631, 799, 836, 1019, 1085
BspANI	GGCC	5 cut(s)	266, 290, 815, 1704, 1841
BspCNI	CTCAG	5 cut(s)	547, 901, 961, 1338, 1756
BspFNI	CGCG	3 cut(s)	26, 137, 269
BspHI	TCATGA	1 cut(s)	792
BspLI	GGNNCC	4 cut(s)	97, 496, 681, 1021
BspMAI	CTGCAG	1 cut(s)	1114
BspMI	ACCTGC	1 cut(s)	1070
BspOI	GCTAGC	1 cut(s)	342
BspPI	GGATC	4 cut(s)	140, 794, 1014, 1027
BspQI	GCTCTTC	1 cut(s)	469
BspT104I	TTCGAA	1 cut(s)	420
BspT107I	GGYRCC	2 cut(s)	95, 679
BspTI	CTTAAG	1 cut(s)	1780
BsrBI	CCGCTC	1 cut(s)	1099
BsrDI	GCAATG	1 cut(s)	1859
BsrFI	RCCGGY	1 cut(s)	17
BsrI	ACTGG	1 cut(s)	823
BssAI	RCCGGY	1 cut(s)	17
BssECI	CCNNGG	2 cut(s)	195, 233
BssMI	GATC	9 cut(s)	118, 132, 244, 412, 631, 799, 836, 1019, 1085
BssNI	GRCGYC	3 cut(s)	54, 96, 747
BssT1I	CCWWGG	1 cut(s)	195
Bst4CI	ACNGT	4 cut(s)	408, 754, 1131, 1207
Bst6I	CTCTTC	2 cut(s)	469, 1004
BstACI	GRCGYC	3 cut(s)	54, 96, 747
BstAFI	CTTAAG	1 cut(s)	1780
BstBI	TTCGAA	1 cut(s)	420
BstDEI	CTNAG	5 cut(s)	534, 888, 948, 1325, 1764
BstDSI	CCRYGG	1 cut(s)	233
BstF5I	GGATG	4 cut(s)	205, 660, 756, 1483
BstFNI	CGCG	3 cut(s)	26, 137, 269
BstH2I	RGCGCY	4 cut(s)	66, 99, 260, 1493
BstHHI	GCGC	6 cut(s)	26, 65, 98, 139, 259, 1492
BstKTI	GATC	9 cut(s)	121, 135, 247, 415, 634, 802, 839, 1022, 1088
BstMAI	GTCTC	3 cut(s)	543, 698, 1754
BstMBI	GATC	9 cut(s)	118, 132, 244, 412, 631, 799, 836, 1019, 1085
BstNSI	RCATGY	3 cut(s)	517, 569, 879
BstSFI	CTRYAG	2 cut(s)	1110, 1653
BstUI	CGCG	3 cut(s)	26, 137, 269
BstV1I	GCAGC	8 cut(s)	126, 988, 1096, 1118, 1124, 1370, 1373, 1468
BstX2I	RGATCY	3 cut(s)	799, 836, 1019
BstYI	RGATCY	3 cut(s)	799, 836, 1019
BsuRI	GGCC	5 cut(s)	266, 290, 815, 1704, 1841
BtgI	CCRYGG	1 cut(s)	233
BtrI	CACGTC	1 cut(s)	375
BtsCI	GGATG	4 cut(s)	205, 660, 756, 1483
BtsI	GCAGTG	1 cut(s)	1242
BtsIMutI	CAGTG	7 cut(s)	750, 816, 911, 956, 1127, 1242, 1604
BveI	ACCTGC	1 cut(s)	1070
CaiI	CAGNNNCTG	1 cut(s)	541
CciI	TCATGA	1 cut(s)	792
CfoI	GCGC	6 cut(s)	26, 65, 98, 139, 259, 1492
Cfr10I	RCCGGY	1 cut(s)	17
Cfr13I	GGNCC	2 cut(s)	495, 1839
CseI	GACGC	4 cut(s)	15, 154, 323, 755
Csp6I	GTAC	1 cut(s)	1143
CviAII	CATG	7 cut(s)	514, 566, 710, 793, 876, 1339, 1591
CviQI	GTAC	1 cut(s)	1143
DdeI	CTNAG	5 cut(s)	534, 888, 948, 1325, 1764
DinI	GGCGCC	1 cut(s)	97
DpnI	GATC	9 cut(s)	120, 134, 246, 414, 633, 801, 838, 1021, 1087
DpnII	GATC	9 cut(s)	118, 132, 244, 412, 631, 799, 836, 1019, 1085
DrdI	GACNNNNNNGTC	2 cut(s)	752, 1615
DseDI	GACNNNNNNGTC	2 cut(s)	752, 1615
EaeI	YGGCCR	2 cut(s)	288, 813
Eam1104I	CTCTTC	2 cut(s)	469, 1004
EarI	CTCTTC	2 cut(s)	469, 1004
EciI	GGCGGA	2 cut(s)	64, 145
Ecl136II	GAGCTC	2 cut(s)	1061, 1816
Eco130I	CCWWGG	1 cut(s)	195
Eco147I	AGGCCT	1 cut(s)	1704
Eco24I	GRGCYC	2 cut(s)	1063, 1818
Eco47I	GGWCC	1 cut(s)	495
Eco47III	AGCGCT	1 cut(s)	1491
Eco53kI	GAGCTC	2 cut(s)	1061, 1816
Eco57I	CTGAAG	2 cut(s)	85, 245
Eco88I	CYCGRG	1 cut(s)	1347
EcoICRI	GAGCTC	2 cut(s)	1061, 1816
EcoO109I	RGGNCCY	1 cut(s)	495
EcoT14I	CCWWGG	1 cut(s)	195
EcoT22I	ATGCAT	1 cut(s)	1729
EcoT38I	GRGCYC	2 cut(s)	1063, 1818
EgeI	GGCGCC	1 cut(s)	97
EheI	GGCGCC	1 cut(s)	97
ErhI	CCWWGG	1 cut(s)	195
FaeI	CATG	7 cut(s)	517, 569, 713, 796, 879, 1342, 1594
FaqI	GGGAC	2 cut(s)	508, 1055
FatI	CATG	7 cut(s)	513, 565, 709, 792, 875, 1338, 1590
FauI	CCCGC	2 cut(s)	1165, 1288
FbaI	TGATCA	1 cut(s)	412
FblI	GTMKAC	1 cut(s)	529
FokI	GGATG	4 cut(s)	212, 667, 743, 1490
FriOI	GRGCYC	2 cut(s)	1063, 1818
FspBI	CTAG	4 cut(s)	294, 339, 941, 1854
GlaI	GCGC	6 cut(s)	25, 64, 97, 138, 258, 1491
GsuI	CTGGAG	1 cut(s)	1886
HaeII	RGCGCY	4 cut(s)	66, 99, 260, 1493
HaeIII	GGCC	5 cut(s)	266, 290, 815, 1704, 1841
HapII	CCGG	2 cut(s)	18, 1184
HgaI	GACGC	4 cut(s)	15, 154, 323, 755
HhaI	GCGC	6 cut(s)	26, 65, 98, 139, 259, 1492
Hin1I	GRCGYC	3 cut(s)	54, 96, 747
Hin1II	CATG	7 cut(s)	517, 569, 713, 796, 879, 1342, 1594
Hin6I	GCGC	6 cut(s)	24, 63, 96, 137, 257, 1490
HinP1I	GCGC	6 cut(s)	24, 63, 96, 137, 257, 1490
HincII	GTYRAC	1 cut(s)	49
HindII	GTYRAC	1 cut(s)	49
HindIII	AAGCTT	3 cut(s)	352, 896, 1073
HinfI	GANTC	5 cut(s)	180, 548, 706, 916, 1577
HpaII	CCGG	2 cut(s)	18, 1184
HphI	GGTGA	2 cut(s)	410, 731
Hpy166II	GTNNAC	3 cut(s)	49, 530, 1001
Hpy188I	TCNGA	7 cut(s)	31, 244, 430, 825, 1328, 1538, 1878
Hpy188III	TCNNGA	9 cut(s)	103, 552, 793, 931, 1006, 1028, 1349, 1419, 1637
Hpy8I	GTNNAC	3 cut(s)	49, 530, 1001
Hpy99I	CGWCG	5 cut(s)	31, 56, 59, 62, 376
HpyAV	CCTTC	8 cut(s)	100, 109, 382, 617, 693, 1317, 1468, 1843
HpyCH4III	ACNGT	4 cut(s)	408, 754, 1131, 1207
HpyCH4IV	ACGT	2 cut(s)	54, 374
HpyF3I	CTNAG	5 cut(s)	534, 888, 948, 1325, 1764
HpySE526I	ACGT	2 cut(s)	54, 374
Hsp92I	GRCGYC	3 cut(s)	54, 96, 747
Hsp92II	CATG	7 cut(s)	517, 569, 713, 796, 879, 1342, 1594
HspAI	GCGC	6 cut(s)	24, 63, 96, 137, 257, 1490
KasI	GGCGCC	1 cut(s)	95
KroI	GCCGGC	1 cut(s)	17
KroNI	GCCGGC	1 cut(s)	19
Ksp22I	TGATCA	1 cut(s)	412
Kzo9I	GATC	9 cut(s)	118, 132, 244, 412, 631, 799, 836, 1019, 1085
LguI	GCTCTTC	1 cut(s)	469
LmnI	GCTCC	7 cut(s)	31, 227, 322, 1096, 1421, 1458, 1867
Lsp1109I	GCAGC	8 cut(s)	126, 988, 1096, 1118, 1124, 1370, 1373, 1468
LweI	GCATC	6 cut(s)	190, 1424, 1468, 1557, 1736, 1879
MaeI	CTAG	4 cut(s)	294, 339, 941, 1854
MaeII	ACGT	2 cut(s)	54, 374
MaeIII	GTNAC	6 cut(s)	250, 311, 1028, 1125, 1237, 1380
MalI	GATC	9 cut(s)	120, 134, 246, 414, 633, 801, 838, 1021, 1087
MbiI	CCGCTC	1 cut(s)	1099
MboI	GATC	9 cut(s)	118, 132, 244, 412, 631, 799, 836, 1019, 1085
MboII	GAAGA	9 cut(s)	204, 207, 452, 486, 737, 1021, 1223, 1483, 1865
MfeI	CAATTG	4 cut(s)	307, 467, 1089, 1585
MflI	RGATCY	3 cut(s)	799, 836, 1019
MhlI	GDGCHC	5 cut(s)	327, 466, 1063, 1463, 1818
MlsI	TGGCCA	2 cut(s)	290, 815
MluNI	TGGCCA	2 cut(s)	290, 815
Mly113I	GGCGCC	1 cut(s)	96
MlyI	GAGTC	4 cut(s)	174, 557, 700, 910
MmeI	TCCRAC	1 cut(s)	9
Mox20I	TGGCCA	2 cut(s)	290, 815
Mph1103I	ATGCAT	1 cut(s)	1729
MroNI	GCCGGC	1 cut(s)	17
MroXI	GAANNNNTTC	1 cut(s)	1446
MscI	TGGCCA	2 cut(s)	290, 815
MseI	TTAA	4 cut(s)	989, 1202, 1220, 1781
MslI	CAYNNNNRTG	2 cut(s)	232, 714
Msp20I	TGGCCA	2 cut(s)	290, 815
MspA1I	CMGCKG	3 cut(s)	81, 1109, 1115
MspCI	CTTAAG	1 cut(s)	1780
MspI	CCGG	2 cut(s)	18, 1184
MunI	CAATTG	4 cut(s)	307, 467, 1089, 1585
Mva1269I	GAATGC	2 cut(s)	1237, 1729
MvnI	CGCG	3 cut(s)	26, 137, 269
NaeI	GCCGGC	1 cut(s)	19
NarI	GGCGCC	1 cut(s)	96
NdeII	GATC	9 cut(s)	118, 132, 244, 412, 631, 799, 836, 1019, 1085
NgoMIV	GCCGGC	1 cut(s)	17
NheI	GCTAGC	1 cut(s)	338
NlaIII	CATG	7 cut(s)	517, 569, 713, 796, 879, 1342, 1594
NlaIV	GGNNCC	4 cut(s)	97, 496, 681, 1021
NmuCI	GTSAC	6 cut(s)	250, 311, 1028, 1125, 1237, 1380
NsiI	ATGCAT	1 cut(s)	1729
NspI	RCATGY	3 cut(s)	517, 569, 879
NspV	TTCGAA	1 cut(s)	420
OliI	CACNNNNGTG	1 cut(s)	232
PaeR7I	CTCGAG	1 cut(s)	1347
PagI	TCATGA	1 cut(s)	792
PceI	AGGCCT	1 cut(s)	1704
PciI	ACATGT	2 cut(s)	513, 875
PciSI	GCTCTTC	1 cut(s)	469
PcsI	WCGNNNNNNNCGW	1 cut(s)	51
PctI	GAATGC	2 cut(s)	1237, 1729
PdiI	GCCGGC	1 cut(s)	19
PdmI	GAANNNNTTC	1 cut(s)	1446
PfeI	GAWTC	1 cut(s)	1577
PflFI	GACNNNGTC	1 cut(s)	56
PleI	GAGTC	4 cut(s)	174, 556, 700, 910
PluTI	GGCGCC	1 cut(s)	99
PpsI	GAGTC	4 cut(s)	174, 556, 700, 910
PpuMI	RGGWCCY	1 cut(s)	495
PscI	ACATGT	2 cut(s)	513, 875
PsiI	TTATAA	1 cut(s)	1286
Psp124BI	GAGCTC	2 cut(s)	1063, 1818
Psp5II	RGGWCCY	1 cut(s)	495
PspN4I	GGNNCC	4 cut(s)	97, 496, 681, 1021
PspPI	GGNCC	2 cut(s)	495, 1839
PspPPI	RGGWCCY	1 cut(s)	495
PsrI	GAACNNNNNNTAC	2 cut(s)	401, 433
PstI	CTGCAG	1 cut(s)	1114
PstNI	CAGNNNCTG	1 cut(s)	541
PsuI	RGATCY	3 cut(s)	799, 836, 1019
PsyI	GACNNNGTC	1 cut(s)	56
PvuII	CAGCTG	1 cut(s)	1109
RsaI	GTAC	1 cut(s)	1144
RsaNI	GTAC	1 cut(s)	1143
RseI	CAYNNNNRTG	2 cut(s)	232, 714
SacI	GAGCTC	2 cut(s)	1063, 1818
SapI	GCTCTTC	1 cut(s)	469
SaqAI	TTAA	4 cut(s)	989, 1202, 1220, 1781
Sau3AI	GATC	9 cut(s)	118, 132, 244, 412, 631, 799, 836, 1019, 1085
Sau96I	GGNCC	2 cut(s)	495, 1839
ScaI	AGTACT	1 cut(s)	1144
SchI	GAGTC	4 cut(s)	174, 557, 700, 910
SduI	GDGCHC	5 cut(s)	327, 466, 1063, 1463, 1818
SfaNI	GCATC	6 cut(s)	190, 1424, 1468, 1557, 1736, 1879
SfcI	CTRYAG	2 cut(s)	1110, 1653
SfoI	GGCGCC	1 cut(s)	97
Sfr274I	CTCGAG	1 cut(s)	1347
SfuI	TTCGAA	1 cut(s)	420
SinI	GGWCC	1 cut(s)	495
SlaI	CTCGAG	1 cut(s)	1347
SmiMI	CAYNNNNRTG	2 cut(s)	232, 714
SmlI	CTYRAG	4 cut(s)	931, 1004, 1347, 1780
SmoI	CTYRAG	4 cut(s)	931, 1004, 1347, 1780
SseBI	AGGCCT	1 cut(s)	1704
SspDI	GGCGCC	1 cut(s)	95
SspMI	CTAG	4 cut(s)	294, 339, 941, 1854
SstI	GAGCTC	2 cut(s)	1063, 1818
StuI	AGGCCT	1 cut(s)	1704
StyI	CCWWGG	1 cut(s)	195
TaaI	ACNGT	4 cut(s)	408, 754, 1131, 1207
TaiI	ACGT	2 cut(s)	57, 377
TaqI	TCGA	8 cut(s)	156, 183, 420, 972, 1057, 1084, 1348, 1711
TatI	WGTACW	1 cut(s)	1142
TauI	GCSGC	5 cut(s)	24, 96, 130, 269, 277
TfiI	GAWTC	1 cut(s)	1577
Tru1I	TTAA	4 cut(s)	989, 1202, 1220, 1781
Tru9I	TTAA	4 cut(s)	989, 1202, 1220, 1781
TscAI	CASTG	7 cut(s)	757, 823, 918, 956, 1134, 1242, 1611
TseFI	GTSAC	6 cut(s)	250, 311, 1028, 1125, 1237, 1380
Tsp45I	GTSAC	6 cut(s)	250, 311, 1028, 1125, 1237, 1380
TspDTI	ATGAA	6 cut(s)	348, 629, 746, 1281, 1614, 1848
TspRI	CASTG	7 cut(s)	757, 823, 918, 956, 1134, 1242, 1611
Tth111I	GACNNNGTC	1 cut(s)	56
Vha464I	CTTAAG	1 cut(s)	1780
VpaK11BI	GGWCC	1 cut(s)	495
XapI	RAATTY	3 cut(s)	185, 502, 904
XceI	RCATGY	3 cut(s)	517, 569, 879
XhoI	CTCGAG	1 cut(s)	1347
XmiI	GTMKAC	1 cut(s)	529
XmnI	GAANNNNTTC	1 cut(s)	1446
XspI	CTAG	4 cut(s)	294, 339, 941, 1854
ZraI	GACGTC	1 cut(s)	55
ZrmI	AGTACT	1 cut(s)	1144
Zsp2I	ATGCAT	1 cut(s)	1729

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.