MD13G1027200.v1.1

ELL-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011867
Physical Location & Seq
Reverse (-)
1963745 .. 1966579
2835 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1027200.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGGCGAGCAACTCCAAGGAAGAGCCCAAGACCGGCCCCCAGCCCAATCGCTGGTACAACCTAACCCTCGGCCCCTCCTTCAAAGACGACTCCGCCAACAAGTACTGCACTCTCCGATACGAATTCAAACCGGCGTCAATCGACAAGACGAAGCCCGGGGCGCTGAAGAAGACCAAGGACAATAGGGTTGCGGTCGAATTTCAGAACAACCAATTGGGGAAACCCAATGTCACGTTCAAGGGGAGCAGCGAGGAGTACAAGGACAACGACGCCGTTTTGTTCTTTGACGGCCAGACGTTTCGGTTGGATCGGCTTCACCGCGCCGTGAAGCAGCTTCGGCATGACCGTCAGCCTGGGGAATCGGCGGCTGGTATGGCCGTGGACCCGCAGCTGTCCCCAGTAGGCAAGGCGCCCAAGCTATCGCATCAGTATCATCCTGGTAGAACTGCATTCCCTGCCGTGCCGGTTGAGGTGGAACGGATAGATGTTGGCGTGCCTGAAAATCCAGGTGCGAAAACTGCCAATAAAGGTGTGGTTGATTACTCATCTGATCGACCAAACGTATCTGCTGCTTCGCCAGGCCCAAAGAATGAAGAAGTTGACGACGAACACCAGGATATAGACATTGAAGACCTTTTTGGTAGTATATCACCGGATGATGGGAATGCCGCTGAAGATGAAGCCAATGCTGGATTTGATATCAATATGCTCCATCAGAATGAAAGTGACAATGAGATTGCTGATGTAGATGATAGCGGTGATGAAGTTGACAAGGGGCCAAATGCTGCAGAAGCCCTTCGAGCCCAGGTGATGAATACCCAGGGAAGGGAAACACACACTGATATTTTTGGTGACGAGGGGAATGCAGAAGGAAGGAAAACCGAGACTTCTAGTTCAAGTAGCAGCAGTGGAAGTAGCAGCGGAAGTGAGAGCAGCAGTAGCGGAAGTAGTAGTGGGAGCAGCAGCAGCAGTGGCAGCGGAAATGGTGGTGGTAATAATGCAGGCAGCGATGACGATTCAGTCAACTCCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EAF

Protein Analysis

345

Amino Acids

36.52

Weight (kDa)

4.65

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EAF PF09816 19 - 118 1.4e-23 RNA polymerase II transcription elongation factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011867)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71080
fragaria_vesca FvH4_4g32210 FvH4_4g32210 FvH4_7g01202
malus_domestica MD13G1027200.v1.1 MD16G1030300.v1.1
prunus_persica Prupe.1G322700_v2.0.a1
pyrus_communis pycom13g02590 pycom16g02580
rosa_chinensis RchiOBHm_Chr4g0440951
rosa_laevigata RLG00000006137
rosa_multiflora Rmu_sc0000424.1_g000025
rosa_roxburghii Rroxscaffold_5G00381810
rosa_rugosa Rorug04G0329600
rosa_samantha Rh4AG381400 Rh4BG394600 Rh4CG409500 Rh4DG387900
rosa_wichuraiana Rw4G032850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1019
AccB1I GGYRCC 1 cut(s) 409
AccB7I CCANNNNNTGG 1 cut(s) 51
AccII CGCG 1 cut(s) 321
AclWI GGATC 1 cut(s) 315
AcoI YGGCCR 2 cut(s) 289, 375
AcsI RAATTY 2 cut(s) 122, 197
AcuI CTGAAG 2 cut(s) 185, 693
AcyI GRCGYC 3 cut(s) 134, 270, 410
AfaI GTAC 3 cut(s) 56, 104, 257
AfiI CCNNNNNNNGG 5 cut(s) 32, 51, 659, 825, 826
AgsI TTSAA 5 cut(s) 82, 127, 238, 629, 897
AjnI CCWGG 7 cut(s) 352, 436, 505, 577, 612, 804, 819
AjuI GAANNNNNNNTTGG 6 cut(s) 197, 218, 229, 250, 621, 653
AluBI AGCT 3 cut(s) 334, 391, 418
AluI AGCT 3 cut(s) 334, 391, 418
Alw26I GTCTC 1 cut(s) 878
AlwI GGATC 1 cut(s) 315
Ama87I CYCGRG 1 cut(s) 155
AoxI GGCC 6 cut(s) 34, 70, 289, 375, 580, 776
ApoI RAATTY 2 cut(s) 122, 197
Asp700I GAANNNNTTC 1 cut(s) 795
AspLEI GCGC 3 cut(s) 163, 323, 412
AspS9I GGNCC 5 cut(s) 35, 71, 382, 581, 776
AsuC2I CCSGG 2 cut(s) 156, 157
AsuHPI GGTGA 5 cut(s) 308, 642, 770, 820, 863
AvaI CYCGRG 1 cut(s) 155
AvaII GGWCC 1 cut(s) 382
BanI GGYRCC 1 cut(s) 409
BanII GRGCYC 2 cut(s) 27, 805
BbsI GAAGAC 2 cut(s) 176, 636
BccI CCATC 2 cut(s) 653, 720
BceAI ACGGC 5 cut(s) 257, 304, 308, 362, 443
BcgI CGANNNNNNTGC 2 cut(s) 845, 879
BciT130I CCWGG 7 cut(s) 354, 438, 507, 579, 614, 806, 821
BcnI CCSGG 2 cut(s) 156, 157
BcoDI GTCTC 1 cut(s) 878
BfaI CTAG 1 cut(s) 891
BfmI CTRYAG 1 cut(s) 786
BfoI RGCGCY 2 cut(s) 164, 413
BmcAI AGTACT 1 cut(s) 104
Bme1390I CCNGG 9 cut(s) 156, 157, 354, 438, 507, 579, 614, 806, 821
Bme18I GGWCC 1 cut(s) 382
BmeT110I CYCGRG 1 cut(s) 155
BmgT120I GGNCC 5 cut(s) 35, 71, 382, 581, 776
BmiI GGNNCC 5 cut(s) 37, 73, 384, 411, 777
BmrFI CCNGG 9 cut(s) 156, 157, 354, 438, 507, 579, 614, 806, 821
BmrI ACTGGG 1 cut(s) 392
BmsI GCATC 1 cut(s) 433
BmuI ACTGGG 1 cut(s) 392
BpiI GAAGAC 2 cut(s) 176, 636
BpuMI CCSGG 2 cut(s) 156, 157
BsaHI GRCGYC 3 cut(s) 134, 270, 410
BsaWI WCCGGW 1 cut(s) 652
Bsc4I CCNNNNNNNGG 5 cut(s) 32, 51, 659, 825, 826
Bse118I RCCGGY 3 cut(s) 32, 130, 463
Bse1I ACTGG 1 cut(s) 398
BseBI CCWGG 7 cut(s) 354, 438, 507, 579, 614, 806, 821
BseGI GGATG 2 cut(s) 433, 661
BseLI CCNNNNNNNGG 5 cut(s) 32, 51, 659, 825, 826
BseNI ACTGG 1 cut(s) 398
BseRI GAGGAG 1 cut(s) 266
BseYI CCCAGC 1 cut(s) 39
BsgI GTGCAG 1 cut(s) 91
Bsh1236I CGCG 1 cut(s) 321
Bsh1285I CGRYCG 1 cut(s) 195
BshFI GGCC 6 cut(s) 36, 72, 291, 377, 582, 778
BshNI GGYRCC 1 cut(s) 409
BsiEI CGRYCG 1 cut(s) 195
BsiHKCI CYCGRG 1 cut(s) 155
BsiSI CCGG 5 cut(s) 33, 131, 156, 464, 653
BslFI GGGAC 1 cut(s) 379
BslI CCNNNNNNNGG 5 cut(s) 32, 51, 659, 825, 826
BsmAI GTCTC 1 cut(s) 878
BsmFI GGGAC 1 cut(s) 379
BsmI GAATGC 3 cut(s) 449, 670, 868
BsnI GGCC 6 cut(s) 36, 72, 291, 377, 582, 778
BsoBI CYCGRG 1 cut(s) 155
Bsp1286I GDGCHC 2 cut(s) 27, 805
Bsp143I GATC 2 cut(s) 307, 550
BspANI GGCC 6 cut(s) 36, 72, 291, 377, 582, 778
BspFNI CGCG 1 cut(s) 321
BspLI GGNNCC 5 cut(s) 37, 73, 384, 411, 777
BspMAI CTGCAG 1 cut(s) 790
BspPI GGATC 1 cut(s) 315
BspQI GCTCTTC 1 cut(s) 15
BspT107I GGYRCC 1 cut(s) 409
BsrFI RCCGGY 3 cut(s) 32, 130, 463
BsrI ACTGG 1 cut(s) 398
BssAI RCCGGY 3 cut(s) 32, 130, 463
BssMI GATC 2 cut(s) 307, 550
BssNI GRCGYC 3 cut(s) 134, 270, 410
BssT1I CCWWGG 2 cut(s) 15, 174
Bst2UI CCWGG 7 cut(s) 354, 438, 507, 579, 614, 806, 821
Bst4CI ACNGT 1 cut(s) 347
Bst6I CTCTTC 1 cut(s) 15
BstACI GRCGYC 3 cut(s) 134, 270, 410
BstAPI GCANNNNNTGC 1 cut(s) 455
BstC8I GCNNGC 3 cut(s) 7, 494, 1003
BstDSI CCRYGG 1 cut(s) 378
BstF5I GGATG 2 cut(s) 433, 661
BstFNI CGCG 1 cut(s) 321
BstH2I RGCGCY 2 cut(s) 164, 413
BstHHI GCGC 3 cut(s) 163, 323, 412
BstKTI GATC 2 cut(s) 310, 553
BstMAI GTCTC 1 cut(s) 878
BstMBI GATC 2 cut(s) 307, 550
BstMCI CGRYCG 1 cut(s) 195
BstNI CCWGG 7 cut(s) 354, 438, 507, 579, 614, 806, 821
BstSCI CCNGG 9 cut(s) 154, 155, 352, 436, 505, 577, 612, 804, 819
BstSFI CTRYAG 1 cut(s) 786
BstUI CGCG 1 cut(s) 321
BstV2I GAAGAC 2 cut(s) 176, 636
BsuRI GGCC 6 cut(s) 36, 72, 291, 377, 582, 778
BtgI CCRYGG 1 cut(s) 378
BtgZI GCGATG 1 cut(s) 1023
BtsCI GGATG 2 cut(s) 433, 661
BtsI GCAGTG 2 cut(s) 913, 976
BtsIMutI CAGTG 3 cut(s) 837, 913, 976
Cac8I GCNNGC 3 cut(s) 7, 494, 1003
CfoI GCGC 3 cut(s) 163, 323, 412
Cfr10I RCCGGY 3 cut(s) 32, 130, 463
Cfr13I GGNCC 5 cut(s) 35, 71, 382, 581, 776
Cfr9I CCCGGG 1 cut(s) 155
CseI GACGC 2 cut(s) 123, 278
Csp6I GTAC 3 cut(s) 55, 103, 256
CviAII CATG 1 cut(s) 341
CviQI GTAC 3 cut(s) 55, 103, 256
DinI GGCGCC 1 cut(s) 411
DpnI GATC 2 cut(s) 309, 552
DpnII GATC 2 cut(s) 307, 550
DrdI GACNNNNNNGTC 1 cut(s) 1019
DseDI GACNNNNNNGTC 1 cut(s) 1019
EaeI YGGCCR 2 cut(s) 289, 375
Eam1104I CTCTTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 15
EciI GGCGGA 1 cut(s) 82
Eco130I CCWWGG 2 cut(s) 15, 174
Eco24I GRGCYC 2 cut(s) 27, 805
Eco32I GATATC 1 cut(s) 700
Eco47I GGWCC 1 cut(s) 382
Eco57I CTGAAG 2 cut(s) 185, 693
Eco88I CYCGRG 1 cut(s) 155
EcoRI GAATTC 1 cut(s) 122
EcoRII CCWGG 7 cut(s) 352, 436, 505, 577, 612, 804, 819
EcoRV GATATC 1 cut(s) 700
EcoT14I CCWWGG 2 cut(s) 15, 174
EcoT38I GRGCYC 2 cut(s) 27, 805
EgeI GGCGCC 1 cut(s) 411
EheI GGCGCC 1 cut(s) 411
ErhI CCWWGG 2 cut(s) 15, 174
FaeI CATG 1 cut(s) 344
FaiI YATR 5 cut(s) 342, 374, 620, 647, 707
FaqI GGGAC 1 cut(s) 379
FatI CATG 1 cut(s) 340
FauI CCCGC 1 cut(s) 393
FokI GGATG 2 cut(s) 420, 668
FriOI GRGCYC 2 cut(s) 27, 805
FspBI CTAG 1 cut(s) 891
GlaI GCGC 3 cut(s) 162, 322, 411
GsaI CCCAGC 1 cut(s) 43
HaeII RGCGCY 2 cut(s) 164, 413
HaeIII GGCC 6 cut(s) 36, 72, 291, 377, 582, 778
HapII CCGG 5 cut(s) 33, 131, 156, 464, 653
HgaI GACGC 2 cut(s) 123, 278
HhaI GCGC 3 cut(s) 163, 323, 412
Hin1I GRCGYC 3 cut(s) 134, 270, 410
Hin1II CATG 1 cut(s) 344
Hin6I GCGC 3 cut(s) 161, 321, 410
HinP1I GCGC 3 cut(s) 161, 321, 410
HincII GTYRAC 3 cut(s) 601, 769, 1024
HindII GTYRAC 3 cut(s) 601, 769, 1024
HinfI GANTC 3 cut(s) 89, 359, 1016
HpaII CCGG 5 cut(s) 33, 131, 156, 464, 653
HphI GGTGA 5 cut(s) 308, 642, 770, 820, 863
Hpy166II GTNNAC 4 cut(s) 382, 601, 769, 1024
Hpy188I TCNGA 5 cut(s) 116, 204, 550, 717, 1034
Hpy8I GTNNAC 4 cut(s) 382, 601, 769, 1024
Hpy99I CGWCG 2 cut(s) 272, 608
HpyAV CCTTC 5 cut(s) 88, 806, 819, 863, 867
HpyCH4III ACNGT 1 cut(s) 347
HpyCH4IV ACGT 3 cut(s) 233, 296, 561
HpyCH4V TGCA 5 cut(s) 108, 449, 788, 866, 1001
HpySE526I ACGT 3 cut(s) 233, 296, 561
Hsp92I GRCGYC 3 cut(s) 134, 270, 410
Hsp92II CATG 1 cut(s) 344
HspAI GCGC 3 cut(s) 161, 321, 410
KasI GGCGCC 1 cut(s) 409
Kzo9I GATC 2 cut(s) 307, 550
LguI GCTCTTC 1 cut(s) 15
LmnI GCTCC 3 cut(s) 243, 714, 957
LweI GCATC 1 cut(s) 433
MaeI CTAG 1 cut(s) 891
MaeII ACGT 3 cut(s) 233, 296, 561
MaeIII GTNAC 3 cut(s) 229, 725, 851
MalI GATC 2 cut(s) 309, 552
MboI GATC 2 cut(s) 307, 550
MboII GAAGA 6 cut(s) 32, 178, 181, 605, 641, 686
MfeI CAATTG 1 cut(s) 212
MhlI GDGCHC 2 cut(s) 27, 805
MluCI AATT 3 cut(s) 122, 197, 212
Mly113I GGCGCC 1 cut(s) 410
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 1 cut(s) 285
MnlI CCTC 5 cut(s) 77, 85, 244, 463, 850
MroXI GAANNNNTTC 1 cut(s) 795
MslI CAYNNNNRTG 1 cut(s) 717
MspA1I CMGCKG 4 cut(s) 391, 671, 921, 978
MspI CCGG 5 cut(s) 33, 131, 156, 464, 653
MspR9I CCNGG 9 cut(s) 156, 157, 354, 438, 507, 579, 614, 806, 821
MunI CAATTG 1 cut(s) 212
Mva1269I GAATGC 3 cut(s) 449, 670, 868
MvaI CCWGG 7 cut(s) 354, 438, 507, 579, 614, 806, 821
MvnI CGCG 1 cut(s) 321
NarI GGCGCC 1 cut(s) 410
NciI CCSGG 2 cut(s) 156, 157
NdeII GATC 2 cut(s) 307, 550
NlaIII CATG 1 cut(s) 344
NlaIV GGNNCC 5 cut(s) 37, 73, 384, 411, 777
NmeAIII GCCGAG 1 cut(s) 48
NmuCI GTSAC 3 cut(s) 229, 725, 851
PasI CCCWGGG 1 cut(s) 820
PciSI GCTCTTC 1 cut(s) 15
PcsI WCGNNNNNNNCGW 1 cut(s) 343
PctI GAATGC 3 cut(s) 449, 670, 868
PdmI GAANNNNTTC 1 cut(s) 795
PfeI GAWTC 2 cut(s) 359, 1016
PflMI CCANNNNNTGG 1 cut(s) 51
PleI GAGTC 1 cut(s) 83
PluTI GGCGCC 1 cut(s) 413
PpsI GAGTC 1 cut(s) 83
Psp6I CCWGG 7 cut(s) 352, 436, 505, 577, 612, 804, 819
PspFI CCCAGC 1 cut(s) 39
PspGI CCWGG 7 cut(s) 352, 436, 505, 577, 612, 804, 819
PspN4I GGNNCC 5 cut(s) 37, 73, 384, 411, 777
PspPI GGNCC 5 cut(s) 35, 71, 382, 581, 776
PstI CTGCAG 1 cut(s) 790
PvuII CAGCTG 1 cut(s) 391
RsaI GTAC 3 cut(s) 56, 104, 257
RsaNI GTAC 3 cut(s) 55, 103, 256
RseI CAYNNNNRTG 1 cut(s) 717
SapI GCTCTTC 1 cut(s) 15
Sau3AI GATC 2 cut(s) 307, 550
Sau96I GGNCC 5 cut(s) 35, 71, 382, 581, 776
ScaI AGTACT 1 cut(s) 104
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 9 cut(s) 156, 157, 354, 438, 507, 579, 614, 806, 821
SduI GDGCHC 2 cut(s) 27, 805
SfaNI GCATC 1 cut(s) 433
SfcI CTRYAG 1 cut(s) 786
SfoI GGCGCC 1 cut(s) 411
SinI GGWCC 1 cut(s) 382
SmaI CCCGGG 1 cut(s) 157
SmiMI CAYNNNNRTG 1 cut(s) 717
Sse9I AATT 3 cut(s) 122, 197, 212
SspDI GGCGCC 1 cut(s) 409
SspMI CTAG 1 cut(s) 891
StyD4I CCNGG 9 cut(s) 154, 155, 352, 436, 505, 577, 612, 804, 819
StyI CCWWGG 2 cut(s) 15, 174
TaaI ACNGT 1 cut(s) 347
TaiI ACGT 3 cut(s) 236, 299, 564
TaqI TCGA 4 cut(s) 141, 195, 553, 799
TasI AATT 3 cut(s) 122, 197, 212
TatI WGTACW 2 cut(s) 102, 255
TauI GCSGC 2 cut(s) 368, 671
TfiI GAWTC 2 cut(s) 359, 1016
TscAI CASTG 3 cut(s) 844, 913, 976
TseFI GTSAC 3 cut(s) 229, 725, 851
Tsp45I GTSAC 3 cut(s) 229, 725, 851
TspDTI ATGAA 5 cut(s) 606, 693, 735, 777, 827
TspGWI ACGGA 1 cut(s) 493
TspMI CCCGGG 1 cut(s) 155
TspRI CASTG 3 cut(s) 844, 913, 976
Van91I CCANNNNNTGG 1 cut(s) 51
VpaK11BI GGWCC 1 cut(s) 382
XapI RAATTY 2 cut(s) 122, 197
XcmI CCANNNNNNNNNTGG 1 cut(s) 529
XmaI CCCGGG 1 cut(s) 155
XmnI GAANNNNTTC 1 cut(s) 795
XspI CTAG 1 cut(s) 891
ZrmI AGTACT 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.