Rh4DG387900

ELL-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0011867
Physical Location & Seq
Forward (+)
60408974 .. 60411898
2925 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG387900.1

Sequence Viewer

Length: 939 bp
ATGGCCAGCAACGCCAAGGAAGAGCCCAAAACCGCTCCTCACCCCGATCGCTGGTACAACCTAACCCTAGGCCCCTCCTTCAAAGACGACTCCTCCAGCAAGTTCTGCACCCTACGATATGAATTTAAGCCGGCGTCGATTGACAAGACGAAGCCGGGGTCGCTGAAGAAGACGAAGGACAATAGGGTTACGGTGGAGTTTCAGAACAACCAATTGGGGAAGCCTAATGTGTTGTTTAAAGGGAATAGTGAGGATTATAAAGACAACGACGCCGTTTTGTTTTTCGACGGCCAGACGTTTCGGCTTGAGAGGCTTCACAGGGCGGTGAAGCAGCTCCGGCATGACCGCCAGCCTGGGGAATCCGCCGGGATTCCTCTGGAGCCGCAGCTGTCTCCGCCGCCCGGCAAGGTGGCCAAGACGATGCATCCTTCTAGAAATTCATTCCCTGCCGTGCCGGTTGAGGTGGAACGAATTGATATTGGTGTGCCAGAGAATTCAGGTGCGAAATCTGTGAGTAAAGGTGTGGCTGATTACTCATTTGATCAACCAAATGTCTCTGCTGCTTCGCCAGACCATAAGGATGATGAAGTTGAGGAACATCAAGATATAGATATTGATGACCTTTTTGGTAGTGGATCACCTGAAGATGGGAATGCCGCTGCTGAAAATAAATCTAATGCTGAATTCGATATCAATATGCCTCATCAGAACGAGATTGATGATGAGATTGCTGATGTAGATGTTAGTGGTGATGAAGTTGATAAGGGCCCAAATGCTGCAGAAGCCCTTCGAGCCCAGGTGAATGCGGAGGAGAGTCATGAGCAGACTTCTTCTTCTAGTAGTAGCAGTGGAAGTGGGAGTAGCAGTGGAAGTGGTAGCACCAGCAGCAGCAGTGACAGTGAAGCCAGCGATGATGATTCTGCAGTCAACTCAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EAF

Protein Analysis

312

Amino Acids

33.61

Weight (kDa)

4.61

Isoelectric Point (pI)

48.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EAF PF09816 19 - 118 2.5e-24 RNA polymerase II transcription elongation factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011867)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71080
fragaria_vesca FvH4_4g32210 FvH4_4g32210 FvH4_7g01202
malus_domestica MD13G1027200.v1.1 MD16G1030300.v1.1
prunus_persica Prupe.1G322700_v2.0.a1
pyrus_communis pycom13g02590 pycom16g02580
rosa_chinensis RchiOBHm_Chr4g0440951
rosa_laevigata RLG00000006137
rosa_multiflora Rmu_sc0000424.1_g000025
rosa_roxburghii Rroxscaffold_5G00381810
rosa_rugosa Rorug04G0329600
rosa_samantha Rh4AG381400 Rh4BG394600 Rh4CG409500 Rh4DG387900
rosa_wichuraiana Rw4G032850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 258
AccBSI CCGCTC 1 cut(s) 35
AciI CCGC 9 cut(s) 33, 323, 346, 363, 383, 395, 398, 657, 806
AclWI GGATC 1 cut(s) 643
AcoI YGGCCR 3 cut(s) 3, 289, 411
AcsI RAATTY 4 cut(s) 122, 436, 493, 683
AcuI CTGAAG 2 cut(s) 185, 663
AcyI GRCGYC 2 cut(s) 134, 270
AfaI GTAC 1 cut(s) 56
AfiI CCNNNNNNNGG 4 cut(s) 51, 355, 401, 647
AgsI TTSAA 1 cut(s) 82
AjnI CCWGG 2 cut(s) 352, 795
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AluBI AGCT 2 cut(s) 334, 388
AluI AGCT 2 cut(s) 334, 388
Alw26I GTCTC 2 cut(s) 396, 559
AlwI GGATC 1 cut(s) 643
AoxI GGCC 5 cut(s) 3, 70, 289, 411, 766
ApaI GGGCCC 1 cut(s) 770
ApeKI GCWGC 7 cut(s) 331, 385, 560, 659, 776, 885, 888
ApoI RAATTY 4 cut(s) 122, 436, 493, 683
ArsI GACNNNNNNTTYG 2 cut(s) 143, 175
Asp700I GAANNNNTTC 1 cut(s) 786
AspA2I CCTAGG 1 cut(s) 67
AspS9I GGNCC 3 cut(s) 71, 766, 767
AsuC2I CCSGG 3 cut(s) 156, 367, 402
AsuHPI GGTGA 5 cut(s) 32, 337, 630, 761, 811
AvrII CCTAGG 1 cut(s) 67
BaeGI GKGCMC 1 cut(s) 770
BalI TGGCCA 2 cut(s) 5, 413
BanII GRGCYC 3 cut(s) 27, 770, 796
BbsI GAAGAC 1 cut(s) 176
BbvI GCAGC 7 cut(s) 343, 397, 547, 646, 763, 897, 900
BccI CCATC 1 cut(s) 641
BceAI ACGGC 3 cut(s) 257, 304, 434
BciT130I CCWGG 2 cut(s) 354, 797
BclI TGATCA 1 cut(s) 541
BcnI CCSGG 3 cut(s) 156, 367, 402
BcoDI GTCTC 2 cut(s) 396, 559
BfaI CTAG 3 cut(s) 68, 432, 837
BfmI CTRYAG 2 cut(s) 777, 921
BlnI CCTAGG 1 cut(s) 67
Bme1390I CCNGG 5 cut(s) 156, 354, 367, 402, 797
BmgT120I GGNCC 3 cut(s) 71, 766, 767
BmiI GGNNCC 3 cut(s) 73, 381, 768
BmrFI CCNGG 5 cut(s) 156, 354, 367, 402, 797
BmsI GCATC 2 cut(s) 411, 433
BpiI GAAGAC 1 cut(s) 176
BpmI CTGGAG 2 cut(s) 79, 398
BpuEI CTTGAG 1 cut(s) 326
BpuMI CCSGG 3 cut(s) 156, 367, 402
BsaHI GRCGYC 2 cut(s) 134, 270
BsaJI CCNNGG 5 cut(s) 15, 67, 155, 353, 795
BsaXI ACNNNNNCTCC 4 cut(s) 77, 107, 850, 880
Bsc4I CCNNNNNNNGG 4 cut(s) 51, 355, 401, 647
Bse118I RCCGGY 2 cut(s) 130, 454
BseBI CCWGG 2 cut(s) 354, 797
BseDI CCNNGG 5 cut(s) 15, 67, 155, 353, 795
BseGI GGATG 2 cut(s) 424, 586
BseLI CCNNNNNNNGG 4 cut(s) 51, 355, 401, 647
BseRI GAGGAG 3 cut(s) 27, 82, 824
BseSI GKGCMC 1 cut(s) 770
BseXI GCAGC 7 cut(s) 343, 397, 547, 646, 763, 897, 900
BsgI GTGCAG 1 cut(s) 91
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 5 cut(s) 5, 72, 291, 413, 768
BsiEI CGRYCG 1 cut(s) 49
BsiSI CCGG 6 cut(s) 131, 155, 337, 366, 402, 455
BslI CCNNNNNNNGG 4 cut(s) 51, 355, 401, 647
BsmAI GTCTC 2 cut(s) 396, 559
BsmI GAATGC 2 cut(s) 658, 808
BsnI GGCC 5 cut(s) 5, 72, 291, 413, 768
Bsp120I GGGCCC 1 cut(s) 766
Bsp1286I GDGCHC 3 cut(s) 27, 770, 796
Bsp143I GATC 3 cut(s) 46, 541, 635
BspACI CCGC 9 cut(s) 33, 323, 346, 363, 383, 395, 398, 657, 806
BspANI GGCC 5 cut(s) 5, 72, 291, 413, 768
BspHI TCATGA 1 cut(s) 817
BspLI GGNNCC 3 cut(s) 73, 381, 768
BspMAI CTGCAG 2 cut(s) 781, 925
BspPI GGATC 1 cut(s) 643
BspQI GCTCTTC 1 cut(s) 15
BsrBI CCGCTC 1 cut(s) 35
BsrFI RCCGGY 2 cut(s) 130, 454
BssAI RCCGGY 2 cut(s) 130, 454
BssECI CCNNGG 5 cut(s) 15, 67, 155, 353, 795
BssMI GATC 3 cut(s) 46, 541, 635
BssNI GRCGYC 2 cut(s) 134, 270
BssT1I CCWWGG 2 cut(s) 15, 67
Bst2UI CCWGG 2 cut(s) 354, 797
Bst4CI ACNGT 2 cut(s) 193, 899
Bst6I CTCTTC 1 cut(s) 15
BstACI GRCGYC 2 cut(s) 134, 270
BstAPI GCANNNNNTGC 1 cut(s) 105
BstC8I GCNNGC 4 cut(s) 7, 132, 350, 907
BstF5I GGATG 2 cut(s) 424, 586
BstKTI GATC 3 cut(s) 49, 544, 638
BstMAI GTCTC 2 cut(s) 396, 559
BstMBI GATC 3 cut(s) 46, 541, 635
BstMCI CGRYCG 1 cut(s) 49
BstMWI GCNNNNNNNGC 9 cut(s) 11, 105, 160, 310, 337, 394, 782, 791, 885
BstNI CCWGG 2 cut(s) 354, 797
BstSCI CCNGG 5 cut(s) 154, 352, 365, 400, 795
BstSFI CTRYAG 2 cut(s) 777, 921
BstSLI GKGCMC 1 cut(s) 770
BstV1I GCAGC 7 cut(s) 343, 397, 547, 646, 763, 897, 900
BstV2I GAAGAC 1 cut(s) 176
BsuRI GGCC 5 cut(s) 5, 72, 291, 413, 768
BtgZI GCGATG 1 cut(s) 924
BtsCI GGATG 2 cut(s) 424, 586
BtsI GCAGTG 3 cut(s) 853, 871, 898
BtsIMutI CAGTG 4 cut(s) 853, 871, 898, 904
Cac8I GCNNGC 4 cut(s) 7, 132, 350, 907
CciI TCATGA 1 cut(s) 817
Cfr10I RCCGGY 2 cut(s) 130, 454
Cfr13I GGNCC 3 cut(s) 71, 766, 767
CseI GACGC 2 cut(s) 123, 278
Csp6I GTAC 1 cut(s) 55
CviAII CATG 2 cut(s) 341, 818
CviQI GTAC 1 cut(s) 55
DpnI GATC 3 cut(s) 48, 543, 637
DpnII GATC 3 cut(s) 46, 541, 635
DraI TTTAAA 1 cut(s) 238
EaeI YGGCCR 3 cut(s) 3, 289, 411
Eam1104I CTCTTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 15
EciI GGCGGA 2 cut(s) 352, 384
Eco130I CCWWGG 2 cut(s) 15, 67
Eco24I GRGCYC 3 cut(s) 27, 770, 796
Eco32I GATATC 1 cut(s) 691
Eco57I CTGAAG 2 cut(s) 185, 663
EcoO109I RGGNCCY 2 cut(s) 71, 766
EcoRI GAATTC 2 cut(s) 493, 683
EcoRII CCWGG 2 cut(s) 352, 795
EcoRV GATATC 1 cut(s) 691
EcoT14I CCWWGG 2 cut(s) 15, 67
EcoT22I ATGCAT 1 cut(s) 426
EcoT38I GRGCYC 3 cut(s) 27, 770, 796
ErhI CCWWGG 2 cut(s) 15, 67
FaeI CATG 2 cut(s) 344, 821
FaiI YATR 7 cut(s) 120, 258, 342, 576, 608, 698, 819
FatI CATG 2 cut(s) 340, 817
FbaI TGATCA 1 cut(s) 541
FokI GGATG 2 cut(s) 411, 593
FriOI GRGCYC 3 cut(s) 27, 770, 796
FspBI CTAG 3 cut(s) 68, 432, 837
GsuI CTGGAG 2 cut(s) 79, 398
HaeIII GGCC 5 cut(s) 5, 72, 291, 413, 768
HapII CCGG 6 cut(s) 131, 155, 337, 366, 402, 455
HgaI GACGC 2 cut(s) 123, 278
Hin1I GRCGYC 2 cut(s) 134, 270
Hin1II CATG 2 cut(s) 344, 821
HincII GTYRAC 1 cut(s) 928
HindII GTYRAC 1 cut(s) 928
HinfI GANTC 5 cut(s) 89, 359, 370, 814, 917
HpaII CCGG 6 cut(s) 131, 155, 337, 366, 402, 455
HphI GGTGA 5 cut(s) 32, 337, 630, 761, 811
Hpy166II GTNNAC 1 cut(s) 928
Hpy188I TCNGA 3 cut(s) 204, 708, 938
Hpy188III TCNNGA 4 cut(s) 377, 432, 602, 818
Hpy8I GTNNAC 1 cut(s) 928
Hpy99I CGWCG 3 cut(s) 139, 272, 290
HpyAV CCTTC 4 cut(s) 88, 169, 438, 797
HpyCH4III ACNGT 2 cut(s) 193, 899
HpyCH4IV ACGT 1 cut(s) 296
HpyCH4V TGCA 4 cut(s) 108, 424, 779, 923
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 105, 160, 310, 337, 394, 782, 791, 885
HpySE526I ACGT 1 cut(s) 296
Hsp92I GRCGYC 2 cut(s) 134, 270
Hsp92II CATG 2 cut(s) 344, 821
KroI GCCGGC 1 cut(s) 130
KroNI GCCGGC 1 cut(s) 132
Ksp22I TGATCA 1 cut(s) 541
Kzo9I GATC 3 cut(s) 46, 541, 635
LguI GCTCTTC 1 cut(s) 15
LmnI GCTCC 3 cut(s) 40, 339, 379
Lsp1109I GCAGC 7 cut(s) 343, 397, 547, 646, 763, 897, 900
LweI GCATC 2 cut(s) 411, 433
MaeI CTAG 3 cut(s) 68, 432, 837
MaeII ACGT 1 cut(s) 296
MaeIII GTNAC 2 cut(s) 187, 893
MalI GATC 3 cut(s) 48, 543, 637
MbiI CCGCTC 1 cut(s) 35
MboI GATC 3 cut(s) 46, 541, 635
MboII GAAGA 6 cut(s) 32, 178, 181, 656, 822, 825
MfeI CAATTG 1 cut(s) 212
MhlI GDGCHC 3 cut(s) 27, 770, 796
MlsI TGGCCA 2 cut(s) 5, 413
MluCI AATT 6 cut(s) 122, 212, 436, 471, 493, 683
MluNI TGGCCA 2 cut(s) 5, 413
MlyI GAGTC 2 cut(s) 83, 823
Mox20I TGGCCA 2 cut(s) 5, 413
Mph1103I ATGCAT 1 cut(s) 426
MroNI GCCGGC 1 cut(s) 130
MroXI GAANNNNTTC 1 cut(s) 786
MscI TGGCCA 2 cut(s) 5, 413
MseI TTAA 2 cut(s) 126, 237
MslI CAYNNNNRTG 1 cut(s) 579
Msp20I TGGCCA 2 cut(s) 5, 413
MspA1I CMGCKG 2 cut(s) 388, 659
MspI CCGG 6 cut(s) 131, 155, 337, 366, 402, 455
MspR9I CCNGG 5 cut(s) 156, 354, 367, 402, 797
MunI CAATTG 1 cut(s) 212
Mva1269I GAATGC 2 cut(s) 658, 808
MvaI CCWGG 2 cut(s) 354, 797
MwoI GCNNNNNNNGC 9 cut(s) 11, 105, 160, 310, 337, 394, 782, 791, 885
NaeI GCCGGC 1 cut(s) 132
NciI CCSGG 3 cut(s) 156, 367, 402
NdeII GATC 3 cut(s) 46, 541, 635
NgoMIV GCCGGC 1 cut(s) 130
NlaIII CATG 2 cut(s) 344, 821
NlaIV GGNNCC 3 cut(s) 73, 381, 768
NmuCI GTSAC 1 cut(s) 893
NsiI ATGCAT 1 cut(s) 426
PagI TCATGA 1 cut(s) 817
PciSI GCTCTTC 1 cut(s) 15
PctI GAATGC 2 cut(s) 658, 808
PdiI GCCGGC 1 cut(s) 132
PdmI GAANNNNTTC 1 cut(s) 786
PfeI GAWTC 3 cut(s) 359, 370, 917
Ple19I CGATCG 1 cut(s) 49
PleI GAGTC 2 cut(s) 83, 822
PpsI GAGTC 2 cut(s) 83, 822
PsiI TTATAA 1 cut(s) 258
Psp6I CCWGG 2 cut(s) 352, 795
PspGI CCWGG 2 cut(s) 352, 795
PspN4I GGNNCC 3 cut(s) 73, 381, 768
PspOMI GGGCCC 1 cut(s) 766
PspPI GGNCC 3 cut(s) 71, 766, 767
PstI CTGCAG 2 cut(s) 781, 925
PvuI CGATCG 1 cut(s) 49
PvuII CAGCTG 1 cut(s) 388
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
RseI CAYNNNNRTG 1 cut(s) 579
SapI GCTCTTC 1 cut(s) 15
SaqAI TTAA 2 cut(s) 126, 237
Sau3AI GATC 3 cut(s) 46, 541, 635
Sau96I GGNCC 3 cut(s) 71, 766, 767
SchI GAGTC 2 cut(s) 83, 823
ScrFI CCNGG 5 cut(s) 156, 354, 367, 402, 797
SduI GDGCHC 3 cut(s) 27, 770, 796
SfaNI GCATC 2 cut(s) 411, 433
SfcI CTRYAG 2 cut(s) 777, 921
SmiMI CAYNNNNRTG 1 cut(s) 579
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
Sse9I AATT 6 cut(s) 122, 212, 436, 471, 493, 683
SsiI CCGC 9 cut(s) 33, 323, 346, 363, 383, 395, 398, 657, 806
SspMI CTAG 3 cut(s) 68, 432, 837
StyD4I CCNGG 5 cut(s) 154, 352, 365, 400, 795
StyI CCWWGG 2 cut(s) 15, 67
TaaI ACNGT 2 cut(s) 193, 899
TaiI ACGT 1 cut(s) 299
TaqI TCGA 4 cut(s) 137, 285, 687, 790
TasI AATT 6 cut(s) 122, 212, 436, 471, 493, 683
TauI GCSGC 3 cut(s) 385, 400, 659
TfiI GAWTC 3 cut(s) 359, 370, 917
Tru1I TTAA 2 cut(s) 126, 237
Tru9I TTAA 2 cut(s) 126, 237
TscAI CASTG 4 cut(s) 853, 871, 898, 904
TseFI GTSAC 1 cut(s) 893
TseI GCWGC 7 cut(s) 331, 385, 560, 659, 776, 885, 888
Tsp45I GTSAC 1 cut(s) 893
TspDTI ATGAA 4 cut(s) 135, 429, 600, 768
TspRI CASTG 4 cut(s) 853, 871, 898, 904
XapI RAATTY 4 cut(s) 122, 436, 493, 683
XbaI TCTAGA 1 cut(s) 431
XmaJI CCTAGG 1 cut(s) 67
XmnI GAANNNNTTC 1 cut(s) 786
XspI CTAG 3 cut(s) 68, 432, 837
Zsp2I ATGCAT 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.