RchiOBHm_Chr4g0440951

ELL-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0011867
Physical Location & Seq
Forward (+)
62863870 .. 62866689
2820 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40887

Sequence Viewer

Length: 1131 bp
ATGGTTTGTCTGTCTTTTGAGACCATATGTGTCCTCTCCCGTCTCCTCCTTCTCCAAAATCCATCCTCACCGGCGCTCACTCCGGTCGTGCCATGTCAGCTAGCCGCGGTGGACCCCACTTCCTCACTCCTTCTCTTCTCCTCACGCGCCTCACCTGAACACCACTCTCCTTCTTCTCCGACAAACCGAACCATGGCCAGCAACGCCAAGGAAGAGCCCAAAACCGCTCCTCATCCCGATCGCTGGTACAACCTAACCCTAGGCCCCTCCTTCAAAGACGACTCCTCCAGCAAGTTCTGCACCCTACGATATGAATTTAAGCCGGCGTCGATTGACAAGACGAAGCCGGGGTCGCTGAAGAAGACGAAGGACAATAGGGTTACGGTGGAGTTTCAGAACAACCAATTGGGGAAGCCTAATGTGTTGTTTAAAGGGAATAGTGAGGATTATAAAGACAACGACGCCGTTTTGTTTTTCGACGGCCAGACGTTTCGGCTTGAGAGGCTTCACAGGGCGGTGAAGCAGCTCCGGCATGACCGCCAGCCTGGGGAATCCGCCGGGATTCCTCTGGAGCCGCAGCTGTCTCCGCCGCCCGGCAAGGTGGCCAAGACGATGCATCCTTCTAGAAATTCATTCCCTGCCGTGCCGGTTGAGGTGGAACGAATTGATATTGGTGTGCCAGAGAATTCAGGTGCGAAATCTGTGAGTAAAGGTGTGGCTGATTACTCATTTGATCAACCAAATGTCTCTGCTGCTTCGCCAGACCATAAGGATGATGAAGTTGAGGAACATCAAGATATAGATATTGATGACCTTTTTGGTAGTGGATCACCTGAAGATGGGAATGCCGCTGCTGAAAATAAATCTAATGCTGAATTCGATATCAATATGCCTCATCAGAACGAGATTGATGATGAGATTGCTGATGTAGATGTTAGTGGTGATGAAGTTGATAAGGGCCCAAATGCTGCAGAAGCCCTTCGAGCCCAGGTGAATGCGGAGGAGAGTCATGAGCAGACTTCTTCTTCTAGTAGTAGCAGTGGAAGTGGGAGTAGCAGTGGAAGTGGTAGCACCAGCAGCAGCAGTGACAGTGAAGCCAGCGATGATGATTCTGCAGTCAACTCAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EAF

Protein Analysis

376

Amino Acids

40.41

Weight (kDa)

4.71

Isoelectric Point (pI)

52.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EAF PF09816 83 - 182 3.6e-24 RNA polymerase II transcription elongation factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011867)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71080
fragaria_vesca FvH4_4g32210 FvH4_4g32210 FvH4_7g01202
malus_domestica MD13G1027200.v1.1 MD16G1030300.v1.1
prunus_persica Prupe.1G322700_v2.0.a1
pyrus_communis pycom13g02590 pycom16g02580
rosa_chinensis RchiOBHm_Chr4g0440951
rosa_laevigata RLG00000006137
rosa_multiflora Rmu_sc0000424.1_g000025
rosa_roxburghii Rroxscaffold_5G00381810
rosa_rugosa Rorug04G0329600
rosa_samantha Rh4AG381400 Rh4BG394600 Rh4CG409500 Rh4DG387900
rosa_wichuraiana Rw4G032850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 450
AccBSI CCGCTC 1 cut(s) 227
AccII CGCG 2 cut(s) 107, 147
AclWI GGATC 1 cut(s) 835
AcoI YGGCCR 3 cut(s) 195, 481, 603
AcsI RAATTY 4 cut(s) 314, 628, 685, 875
AcuI CTGAAG 2 cut(s) 377, 855
AcyI GRCGYC 2 cut(s) 326, 462
AfaI GTAC 1 cut(s) 248
AfiI CCNNNNNNNGG 5 cut(s) 193, 243, 547, 593, 839
AgsI TTSAA 1 cut(s) 274
AjnI CCWGG 2 cut(s) 544, 987
AjuI GAANNNNNNNTTGG 2 cut(s) 389, 421
AluBI AGCT 3 cut(s) 100, 526, 580
AluI AGCT 3 cut(s) 100, 526, 580
Alw26I GTCTC 4 cut(s) 14, 47, 588, 751
AlwI GGATC 1 cut(s) 835
AoxI GGCC 5 cut(s) 195, 262, 481, 603, 958
ApaI GGGCCC 1 cut(s) 962
ApeKI GCWGC 7 cut(s) 523, 577, 752, 851, 968, 1077, 1080
ApoI RAATTY 4 cut(s) 314, 628, 685, 875
ArsI GACNNNNNNTTYG 2 cut(s) 335, 367
Asp700I GAANNNNTTC 1 cut(s) 978
AspA2I CCTAGG 1 cut(s) 259
AspLEI GCGC 2 cut(s) 76, 149
AspS9I GGNCC 4 cut(s) 112, 263, 958, 959
AsuC2I CCSGG 3 cut(s) 348, 559, 594
AsuHPI GGTGA 6 cut(s) 60, 144, 529, 822, 953, 1003
AsuNHI GCTAGC 1 cut(s) 100
AvaII GGWCC 1 cut(s) 112
AvrII CCTAGG 1 cut(s) 259
BaeGI GKGCMC 1 cut(s) 962
BalI TGGCCA 2 cut(s) 197, 605
BanII GRGCYC 3 cut(s) 219, 962, 988
BbsI GAAGAC 1 cut(s) 368
BbvI GCAGC 7 cut(s) 535, 589, 739, 838, 955, 1089, 1092
BccI CCATC 2 cut(s) 70, 833
BceAI ACGGC 3 cut(s) 449, 496, 626
BciT130I CCWGG 2 cut(s) 546, 989
BclI TGATCA 1 cut(s) 733
BcnI CCSGG 3 cut(s) 348, 559, 594
BcoDI GTCTC 4 cut(s) 14, 47, 588, 751
BfaI CTAG 4 cut(s) 101, 260, 624, 1029
BfmI CTRYAG 2 cut(s) 969, 1113
BfoI RGCGCY 1 cut(s) 77
BlnI CCTAGG 1 cut(s) 259
Bme1390I CCNGG 5 cut(s) 348, 546, 559, 594, 989
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 4 cut(s) 112, 263, 958, 959
BmiI GGNNCC 4 cut(s) 114, 265, 573, 960
BmrFI CCNGG 5 cut(s) 348, 546, 559, 594, 989
BmsI GCATC 2 cut(s) 603, 625
BmtI GCTAGC 1 cut(s) 104
BpiI GAAGAC 1 cut(s) 368
BpmI CTGGAG 2 cut(s) 271, 590
BpuEI CTTGAG 1 cut(s) 518
BpuMI CCSGG 3 cut(s) 348, 559, 594
BsaHI GRCGYC 2 cut(s) 326, 462
BsaI GGTCTC 1 cut(s) 14
BsaJI CCNNGG 7 cut(s) 105, 192, 207, 259, 347, 545, 987
BsaWI WCCGGW 1 cut(s) 82
BsaXI ACNNNNNCTCC 4 cut(s) 269, 299, 1042, 1072
Bsc4I CCNNNNNNNGG 5 cut(s) 193, 243, 547, 593, 839
Bse118I RCCGGY 3 cut(s) 70, 322, 646
BseBI CCWGG 2 cut(s) 546, 989
BseDI CCNNGG 7 cut(s) 105, 192, 207, 259, 347, 545, 987
BseGI GGATG 4 cut(s) 62, 232, 616, 778
BseLI CCNNNNNNNGG 5 cut(s) 193, 243, 547, 593, 839
BseRI GAGGAG 5 cut(s) 35, 130, 219, 274, 1016
BseSI GKGCMC 1 cut(s) 962
BseXI GCAGC 7 cut(s) 535, 589, 739, 838, 955, 1089, 1092
BsgI GTGCAG 1 cut(s) 283
Bsh1236I CGCG 2 cut(s) 107, 147
Bsh1285I CGRYCG 2 cut(s) 87, 241
BshFI GGCC 5 cut(s) 197, 264, 483, 605, 960
BsiEI CGRYCG 2 cut(s) 87, 241
BsiSI CCGG 8 cut(s) 71, 83, 323, 347, 529, 558, 594, 647
BslI CCNNNNNNNGG 5 cut(s) 193, 243, 547, 593, 839
BsmAI GTCTC 4 cut(s) 14, 47, 588, 751
BsmBI CGTCTC 1 cut(s) 47
BsmI GAATGC 2 cut(s) 850, 1000
BsnI GGCC 5 cut(s) 197, 264, 483, 605, 960
Bso31I GGTCTC 1 cut(s) 14
Bsp120I GGGCCC 1 cut(s) 958
Bsp1286I GDGCHC 3 cut(s) 219, 962, 988
Bsp143I GATC 3 cut(s) 238, 733, 827
Bsp19I CCATGG 1 cut(s) 192
BspANI GGCC 5 cut(s) 197, 264, 483, 605, 960
BspFNI CGCG 2 cut(s) 107, 147
BspHI TCATGA 1 cut(s) 1009
BspLI GGNNCC 4 cut(s) 114, 265, 573, 960
BspMAI CTGCAG 2 cut(s) 973, 1117
BspOI GCTAGC 1 cut(s) 104
BspPI GGATC 1 cut(s) 835
BspQI GCTCTTC 1 cut(s) 207
BspTNI GGTCTC 1 cut(s) 14
BsrBI CCGCTC 1 cut(s) 227
BsrFI RCCGGY 3 cut(s) 70, 322, 646
BssAI RCCGGY 3 cut(s) 70, 322, 646
BssECI CCNNGG 7 cut(s) 105, 192, 207, 259, 347, 545, 987
BssMI GATC 3 cut(s) 238, 733, 827
BssNI GRCGYC 2 cut(s) 326, 462
BssT1I CCWWGG 3 cut(s) 192, 207, 259
Bst2UI CCWGG 2 cut(s) 546, 989
Bst4CI ACNGT 2 cut(s) 385, 1091
Bst6I CTCTTC 2 cut(s) 140, 207
BstACI GRCGYC 2 cut(s) 326, 462
BstAPI GCANNNNNTGC 1 cut(s) 297
BstC8I GCNNGC 5 cut(s) 102, 199, 324, 542, 1099
BstDSI CCRYGG 2 cut(s) 105, 192
BstF5I GGATG 4 cut(s) 62, 232, 616, 778
BstFNI CGCG 2 cut(s) 107, 147
BstH2I RGCGCY 1 cut(s) 77
BstHHI GCGC 2 cut(s) 76, 149
BstKTI GATC 3 cut(s) 241, 736, 830
BstMAI GTCTC 4 cut(s) 14, 47, 588, 751
BstMBI GATC 3 cut(s) 238, 733, 827
BstMCI CGRYCG 2 cut(s) 87, 241
BstNI CCWGG 2 cut(s) 546, 989
BstSCI CCNGG 5 cut(s) 346, 544, 557, 592, 987
BstSFI CTRYAG 2 cut(s) 969, 1113
BstSLI GKGCMC 1 cut(s) 962
BstUI CGCG 2 cut(s) 107, 147
BstV1I GCAGC 7 cut(s) 535, 589, 739, 838, 955, 1089, 1092
BstV2I GAAGAC 1 cut(s) 368
BsuRI GGCC 5 cut(s) 197, 264, 483, 605, 960
BtgI CCRYGG 2 cut(s) 105, 192
BtgZI GCGATG 1 cut(s) 1116
BtsCI GGATG 4 cut(s) 62, 232, 616, 778
BtsI GCAGTG 3 cut(s) 1045, 1063, 1090
BtsIMutI CAGTG 4 cut(s) 1045, 1063, 1090, 1096
Cac8I GCNNGC 5 cut(s) 102, 199, 324, 542, 1099
CciI TCATGA 1 cut(s) 1009
CfoI GCGC 2 cut(s) 76, 149
Cfr10I RCCGGY 3 cut(s) 70, 322, 646
Cfr13I GGNCC 4 cut(s) 112, 263, 958, 959
Cfr42I CCGCGG 1 cut(s) 108
CseI GACGC 2 cut(s) 315, 470
Csp6I GTAC 1 cut(s) 247
CviAII CATG 4 cut(s) 93, 193, 533, 1010
CviQI GTAC 1 cut(s) 247
DpnI GATC 3 cut(s) 240, 735, 829
DpnII GATC 3 cut(s) 238, 733, 827
DraI TTTAAA 1 cut(s) 430
EaeI YGGCCR 3 cut(s) 195, 481, 603
Eam1104I CTCTTC 2 cut(s) 140, 207
EarI CTCTTC 2 cut(s) 140, 207
EciI GGCGGA 2 cut(s) 544, 576
Eco130I CCWWGG 3 cut(s) 192, 207, 259
Eco24I GRGCYC 3 cut(s) 219, 962, 988
Eco31I GGTCTC 1 cut(s) 14
Eco32I GATATC 1 cut(s) 883
Eco47I GGWCC 1 cut(s) 112
Eco57I CTGAAG 2 cut(s) 377, 855
EcoO109I RGGNCCY 2 cut(s) 263, 958
EcoRI GAATTC 2 cut(s) 685, 875
EcoRII CCWGG 2 cut(s) 544, 987
EcoRV GATATC 1 cut(s) 883
EcoT14I CCWWGG 3 cut(s) 192, 207, 259
EcoT22I ATGCAT 1 cut(s) 618
EcoT38I GRGCYC 3 cut(s) 219, 962, 988
ErhI CCWWGG 3 cut(s) 192, 207, 259
Esp3I CGTCTC 1 cut(s) 47
FaeI CATG 4 cut(s) 96, 196, 536, 1013
FatI CATG 4 cut(s) 92, 192, 532, 1009
FauNDI CATATG 1 cut(s) 26
FbaI TGATCA 1 cut(s) 733
FokI GGATG 4 cut(s) 49, 219, 603, 785
FriOI GRGCYC 3 cut(s) 219, 962, 988
FspBI CTAG 4 cut(s) 101, 260, 624, 1029
GlaI GCGC 2 cut(s) 75, 148
GsuI CTGGAG 2 cut(s) 271, 590
HaeII RGCGCY 1 cut(s) 77
HaeIII GGCC 5 cut(s) 197, 264, 483, 605, 960
HapII CCGG 8 cut(s) 71, 83, 323, 347, 529, 558, 594, 647
HgaI GACGC 2 cut(s) 315, 470
HhaI GCGC 2 cut(s) 76, 149
Hin1I GRCGYC 2 cut(s) 326, 462
Hin1II CATG 4 cut(s) 96, 196, 536, 1013
Hin6I GCGC 2 cut(s) 74, 147
HinP1I GCGC 2 cut(s) 74, 147
HincII GTYRAC 1 cut(s) 1120
HindII GTYRAC 1 cut(s) 1120
HinfI GANTC 5 cut(s) 281, 551, 562, 1006, 1109
HpaII CCGG 8 cut(s) 71, 83, 323, 347, 529, 558, 594, 647
HphI GGTGA 6 cut(s) 60, 144, 529, 822, 953, 1003
Hpy166II GTNNAC 2 cut(s) 112, 1120
Hpy188I TCNGA 4 cut(s) 180, 396, 900, 1130
Hpy188III TCNNGA 5 cut(s) 236, 569, 624, 794, 1010
Hpy8I GTNNAC 2 cut(s) 112, 1120
Hpy99I CGWCG 3 cut(s) 331, 464, 482
HpyAV CCTTC 7 cut(s) 59, 140, 180, 280, 361, 630, 989
HpyCH4III ACNGT 2 cut(s) 385, 1091
HpyCH4IV ACGT 1 cut(s) 488
HpyCH4V TGCA 4 cut(s) 300, 616, 971, 1115
HpySE526I ACGT 1 cut(s) 488
Hsp92I GRCGYC 2 cut(s) 326, 462
Hsp92II CATG 4 cut(s) 96, 196, 536, 1013
HspAI GCGC 2 cut(s) 74, 147
KroI GCCGGC 1 cut(s) 322
KroNI GCCGGC 1 cut(s) 324
Ksp22I TGATCA 1 cut(s) 733
KspI CCGCGG 1 cut(s) 108
Kzo9I GATC 3 cut(s) 238, 733, 827
LguI GCTCTTC 1 cut(s) 207
LmnI GCTCC 3 cut(s) 232, 531, 571
Lsp1109I GCAGC 7 cut(s) 535, 589, 739, 838, 955, 1089, 1092
LweI GCATC 2 cut(s) 603, 625
MaeI CTAG 4 cut(s) 101, 260, 624, 1029
MaeII ACGT 1 cut(s) 488
MaeIII GTNAC 2 cut(s) 379, 1085
MalI GATC 3 cut(s) 240, 735, 829
MbiI CCGCTC 1 cut(s) 227
MboI GATC 3 cut(s) 238, 733, 827
MboII GAAGA 8 cut(s) 127, 165, 224, 370, 373, 848, 1014, 1017
MfeI CAATTG 1 cut(s) 404
MhlI GDGCHC 3 cut(s) 219, 962, 988
MlsI TGGCCA 2 cut(s) 197, 605
MluCI AATT 6 cut(s) 314, 404, 628, 663, 685, 875
MluNI TGGCCA 2 cut(s) 197, 605
MlyI GAGTC 2 cut(s) 275, 1015
MmeI TCCRAC 1 cut(s) 203
Mox20I TGGCCA 2 cut(s) 197, 605
Mph1103I ATGCAT 1 cut(s) 618
MroNI GCCGGC 1 cut(s) 322
MroXI GAANNNNTTC 1 cut(s) 978
MscI TGGCCA 2 cut(s) 197, 605
MseI TTAA 2 cut(s) 318, 429
MslI CAYNNNNRTG 1 cut(s) 771
Msp20I TGGCCA 2 cut(s) 197, 605
MspA1I CMGCKG 3 cut(s) 107, 580, 851
MspI CCGG 8 cut(s) 71, 83, 323, 347, 529, 558, 594, 647
MspR9I CCNGG 5 cut(s) 348, 546, 559, 594, 989
MunI CAATTG 1 cut(s) 404
Mva1269I GAATGC 2 cut(s) 850, 1000
MvaI CCWGG 2 cut(s) 546, 989
MvnI CGCG 2 cut(s) 107, 147
NaeI GCCGGC 1 cut(s) 324
NciI CCSGG 3 cut(s) 348, 559, 594
NcoI CCATGG 1 cut(s) 192
NdeI CATATG 1 cut(s) 26
NdeII GATC 3 cut(s) 238, 733, 827
NgoMIV GCCGGC 1 cut(s) 322
NheI GCTAGC 1 cut(s) 100
NlaIII CATG 4 cut(s) 96, 196, 536, 1013
NlaIV GGNNCC 4 cut(s) 114, 265, 573, 960
NmuCI GTSAC 1 cut(s) 1085
NsiI ATGCAT 1 cut(s) 618
PagI TCATGA 1 cut(s) 1009
PciSI GCTCTTC 1 cut(s) 207
PctI GAATGC 2 cut(s) 850, 1000
PdiI GCCGGC 1 cut(s) 324
PdmI GAANNNNTTC 1 cut(s) 978
PfeI GAWTC 3 cut(s) 551, 562, 1109
Ple19I CGATCG 1 cut(s) 241
PleI GAGTC 2 cut(s) 275, 1014
PpsI GAGTC 2 cut(s) 275, 1014
PsiI TTATAA 1 cut(s) 450
Psp6I CCWGG 2 cut(s) 544, 987
PspGI CCWGG 2 cut(s) 544, 987
PspN4I GGNNCC 4 cut(s) 114, 265, 573, 960
PspOMI GGGCCC 1 cut(s) 958
PspPI GGNCC 4 cut(s) 112, 263, 958, 959
PstI CTGCAG 2 cut(s) 973, 1117
PvuI CGATCG 1 cut(s) 241
PvuII CAGCTG 1 cut(s) 580
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
RseI CAYNNNNRTG 1 cut(s) 771
SacII CCGCGG 1 cut(s) 108
SapI GCTCTTC 1 cut(s) 207
SaqAI TTAA 2 cut(s) 318, 429
Sau3AI GATC 3 cut(s) 238, 733, 827
Sau96I GGNCC 4 cut(s) 112, 263, 958, 959
SchI GAGTC 2 cut(s) 275, 1015
ScrFI CCNGG 5 cut(s) 348, 546, 559, 594, 989
SduI GDGCHC 3 cut(s) 219, 962, 988
SfaNI GCATC 2 cut(s) 603, 625
SfcI CTRYAG 2 cut(s) 969, 1113
Sfr303I CCGCGG 1 cut(s) 108
SgrAI CRCCGGYG 1 cut(s) 70
SgrBI CCGCGG 1 cut(s) 108
SinI GGWCC 1 cut(s) 112
SmiMI CAYNNNNRTG 1 cut(s) 771
SmlI CTYRAG 1 cut(s) 497
SmoI CTYRAG 1 cut(s) 497
Sse9I AATT 6 cut(s) 314, 404, 628, 663, 685, 875
SspMI CTAG 4 cut(s) 101, 260, 624, 1029
StyD4I CCNGG 5 cut(s) 346, 544, 557, 592, 987
StyI CCWWGG 3 cut(s) 192, 207, 259
TaaI ACNGT 2 cut(s) 385, 1091
TaiI ACGT 1 cut(s) 491
TaqI TCGA 4 cut(s) 329, 477, 879, 982
TasI AATT 6 cut(s) 314, 404, 628, 663, 685, 875
TauI GCSGC 4 cut(s) 107, 577, 592, 851
TfiI GAWTC 3 cut(s) 551, 562, 1109
Tru1I TTAA 2 cut(s) 318, 429
Tru9I TTAA 2 cut(s) 318, 429
TscAI CASTG 4 cut(s) 1045, 1063, 1090, 1096
TseFI GTSAC 1 cut(s) 1085
TseI GCWGC 7 cut(s) 523, 577, 752, 851, 968, 1077, 1080
Tsp45I GTSAC 1 cut(s) 1085
TspDTI ATGAA 4 cut(s) 327, 621, 792, 960
TspRI CASTG 4 cut(s) 1045, 1063, 1090, 1096
VpaK11BI GGWCC 1 cut(s) 112
XapI RAATTY 4 cut(s) 314, 628, 685, 875
XbaI TCTAGA 1 cut(s) 623
XmaJI CCTAGG 1 cut(s) 259
XmnI GAANNNNTTC 1 cut(s) 978
XspI CTAG 4 cut(s) 101, 260, 624, 1029
Zsp2I ATGCAT 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.