MD16G1030300.v1.1

ELL-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011867
Physical Location & Seq
Reverse (-)
2166038 .. 2168987
2950 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1030300.v1.1.491

Sequence Viewer

Length: 1038 bp
ATGGCGAGCAACTCCAAGGAAGAGCCCAAGACCGGCCCCCAGTCCAATTGCTGGTACAACCTAACCCTCGGCCCCTCCTTCAGAGACGGCTCCGAGAACAAGTACTGCACTCTTCGATACGACTTCAAGCCGGCGTCGATCGACAAGACTAAGCCCGGGGCGCTGAAGAAGACCAAGGACAATAGGGTTACGGTCGAATTCCAGAACAACCAAGTGGGGAAACCCAATGTCACGTTTAAAGGGAGCAGCGAGGAGTACAAGGGCAACGACGCCGTATTATTCTTAGACGGCCAGACGTTTCGGTTGGAGAGGCTTCACCGCTCCGTGAAGCAGCTCCGGCATGACCGCCAGCCTGGGGAATCGGCGGCTGGTATGGCCGTGGACCCGCAGCTTTCTCCGGTGGGCAAGGCGCCCAAGCTAACGCATCAGTATAATCCTGCTAGAACTGCATTCCCCGTCGTGCCGGTTGAGGTGGAACGGATAGATGTTGGCGTGCCTGAGAATCCAGGTGCGAAAGCTGCCAATAAAGGTGTCGTTGATTACTCATCTGATCAACCAAATGTATCTGCTGCCTCGCCAGGCCCTAAGAATGAAGAAGTTGACAACAAACACCAGGATATAGATATTGAAGACCTTTTCGGTAGTATATCACCCGATGATGGGAATGCTGCCGAAGATGAAGCCAATCCTGGATTTGATACCAATGTGCCACATCAGAATGAAAGTGATAATGAGATTGCTGATGTAGATGATAGCGGTGATGAAGTTGACAAGGGGCCAAATGCCGCAGCAGCCCTTCGAGCCCAGGTGATGAATGCACAGGGGAGGGAAGCACACTCTGATATTTTTGGAGACGAGGGGAATGCAGAAGGGAGGAAAACCGAGACTTCTAGTTCAAGTAGCAGCAGCAGAAGTAGCAGCGGAGGTGCGAGCAGCAGTAGCAGAAGTAGTAGTGGGAGCAGCAGCAGCAGCAGCAGCGGCAGCGAAAATGGTGGTAATAATGCAGGCAGTGATGACGATTCAGTCAACTCCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EAF

Protein Analysis

346

Amino Acids

36.53

Weight (kDa)

4.78

Isoelectric Point (pI)

48.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EAF PF09816 19 - 118 3.3e-23 RNA polymerase II transcription elongation factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011867)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71080
fragaria_vesca FvH4_4g32210 FvH4_4g32210 FvH4_7g01202
malus_domestica MD13G1027200.v1.1 MD16G1030300.v1.1
prunus_persica Prupe.1G322700_v2.0.a1
pyrus_communis pycom13g02590 pycom16g02580
rosa_chinensis RchiOBHm_Chr4g0440951
rosa_laevigata RLG00000006137
rosa_multiflora Rmu_sc0000424.1_g000025
rosa_roxburghii Rroxscaffold_5G00381810
rosa_rugosa Rorug04G0329600
rosa_samantha Rh4AG381400 Rh4BG394600 Rh4CG409500 Rh4DG387900
rosa_wichuraiana Rw4G032850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1022
AccB1I GGYRCC 1 cut(s) 409
AccB7I CCANNNNNTGG 1 cut(s) 51
AccBSI CCGCTC 1 cut(s) 321
AciI CCGC 8 cut(s) 319, 346, 365, 386, 756, 786, 921, 978
AcoI YGGCCR 2 cut(s) 289, 375
AcsI RAATTY 1 cut(s) 197
AcuI CTGAAG 2 cut(s) 64, 185
AcyI GRCGYC 3 cut(s) 134, 270, 410
AfaI GTAC 3 cut(s) 56, 104, 257
AfiI CCNNNNNNNGG 5 cut(s) 32, 51, 355, 659, 660
AgsI TTSAA 3 cut(s) 127, 629, 897
AjnI CCWGG 6 cut(s) 352, 505, 577, 612, 688, 804
AluBI AGCT 4 cut(s) 334, 391, 418, 518
AluI AGCT 4 cut(s) 334, 391, 418, 518
Alw26I GTCTC 3 cut(s) 78, 846, 878
Ama87I CYCGRG 1 cut(s) 155
AoxI GGCC 6 cut(s) 34, 70, 289, 375, 580, 776
ApoI RAATTY 1 cut(s) 197
AspLEI GCGC 2 cut(s) 163, 412
AspS9I GGNCC 5 cut(s) 35, 71, 382, 581, 776
AsuC2I CCSGG 2 cut(s) 156, 157
AsuHPI GGTGA 4 cut(s) 308, 642, 770, 820
AvaI CYCGRG 1 cut(s) 155
AvaII GGWCC 1 cut(s) 382
BaeI ACNNNNGTAYC 2 cut(s) 690, 723
BanI GGYRCC 1 cut(s) 409
BanII GRGCYC 2 cut(s) 27, 805
BbsI GAAGAC 2 cut(s) 176, 636
BccI CCATC 1 cut(s) 653
BceAI ACGGC 4 cut(s) 103, 257, 304, 362
BcgI CGANNNNNNTGC 2 cut(s) 845, 879
BciT130I CCWGG 6 cut(s) 354, 507, 579, 614, 690, 806
BclI TGATCA 1 cut(s) 550
BcnI CCSGG 2 cut(s) 156, 157
BcoDI GTCTC 3 cut(s) 78, 846, 878
BfaI CTAG 2 cut(s) 441, 891
BfoI RGCGCY 2 cut(s) 164, 413
BmcAI AGTACT 1 cut(s) 104
Bme1390I CCNGG 8 cut(s) 156, 157, 354, 507, 579, 614, 690, 806
Bme18I GGWCC 1 cut(s) 382
BmeT110I CYCGRG 1 cut(s) 155
BmgT120I GGNCC 5 cut(s) 35, 71, 382, 581, 776
BmiI GGNNCC 6 cut(s) 37, 73, 91, 384, 411, 777
BmrFI CCNGG 8 cut(s) 156, 157, 354, 507, 579, 614, 690, 806
BmrI ACTGGG 1 cut(s) 34
BmsI GCATC 1 cut(s) 433
BmuI ACTGGG 1 cut(s) 34
BpiI GAAGAC 2 cut(s) 176, 636
BpuMI CCSGG 2 cut(s) 156, 157
BsaHI GRCGYC 3 cut(s) 134, 270, 410
BsaJI CCNNGG 8 cut(s) 15, 67, 155, 156, 174, 353, 378, 804
BsaWI WCCGGW 1 cut(s) 397
Bsc4I CCNNNNNNNGG 5 cut(s) 32, 51, 355, 659, 660
Bse118I RCCGGY 3 cut(s) 32, 130, 463
Bse1I ACTGG 1 cut(s) 40
BseBI CCWGG 6 cut(s) 354, 507, 579, 614, 690, 806
BseDI CCNNGG 8 cut(s) 15, 67, 155, 156, 174, 353, 378, 804
BseLI CCNNNNNNNGG 5 cut(s) 32, 51, 355, 659, 660
BseMII CTCAG 1 cut(s) 489
BseNI ACTGG 1 cut(s) 40
BseRI GAGGAG 1 cut(s) 266
BsgI GTGCAG 1 cut(s) 91
Bsh1285I CGRYCG 2 cut(s) 141, 195
BshFI GGCC 6 cut(s) 36, 72, 291, 377, 582, 778
BshNI GGYRCC 1 cut(s) 409
BsiEI CGRYCG 2 cut(s) 141, 195
BsiHKCI CYCGRG 1 cut(s) 155
BsiSI CCGG 6 cut(s) 33, 131, 156, 337, 398, 464
BslI CCNNNNNNNGG 5 cut(s) 32, 51, 355, 659, 660
BsmAI GTCTC 3 cut(s) 78, 846, 878
BsmBI CGTCTC 2 cut(s) 78, 846
BsmI GAATGC 4 cut(s) 449, 670, 820, 868
BsnI GGCC 6 cut(s) 36, 72, 291, 377, 582, 778
BsoBI CYCGRG 1 cut(s) 155
Bsp1286I GDGCHC 2 cut(s) 27, 805
Bsp143I GATC 2 cut(s) 138, 550
BspACI CCGC 8 cut(s) 319, 346, 365, 386, 756, 786, 921, 978
BspANI GGCC 6 cut(s) 36, 72, 291, 377, 582, 778
BspCNI CTCAG 1 cut(s) 490
BspLI GGNNCC 6 cut(s) 37, 73, 91, 384, 411, 777
BspQI GCTCTTC 1 cut(s) 15
BspT107I GGYRCC 1 cut(s) 409
BsrBI CCGCTC 1 cut(s) 321
BsrFI RCCGGY 3 cut(s) 32, 130, 463
BsrI ACTGG 1 cut(s) 40
BssAI RCCGGY 3 cut(s) 32, 130, 463
BssECI CCNNGG 8 cut(s) 15, 67, 155, 156, 174, 353, 378, 804
BssMI GATC 2 cut(s) 138, 550
BssNI GRCGYC 3 cut(s) 134, 270, 410
BssT1I CCWWGG 2 cut(s) 15, 174
Bst2UI CCWGG 6 cut(s) 354, 507, 579, 614, 690, 806
Bst4CI ACNGT 1 cut(s) 193
Bst6I CTCTTC 2 cut(s) 15, 117
BstACI GRCGYC 3 cut(s) 134, 270, 410
BstC8I GCNNGC 6 cut(s) 7, 132, 350, 494, 931, 1006
BstDEI CTNAG 4 cut(s) 150, 283, 498, 585
BstDSI CCRYGG 1 cut(s) 378
BstH2I RGCGCY 2 cut(s) 164, 413
BstHHI GCGC 2 cut(s) 163, 412
BstKTI GATC 2 cut(s) 141, 553
BstMAI GTCTC 3 cut(s) 78, 846, 878
BstMBI GATC 2 cut(s) 138, 550
BstMCI CGRYCG 2 cut(s) 141, 195
BstNI CCWGG 6 cut(s) 354, 507, 579, 614, 690, 806
BstSCI CCNGG 8 cut(s) 154, 155, 352, 505, 577, 612, 688, 804
BstV2I GAAGAC 2 cut(s) 176, 636
BsuRI GGCC 6 cut(s) 36, 72, 291, 377, 582, 778
BtgI CCRYGG 1 cut(s) 378
BtsI GCAGTG 1 cut(s) 1015
BtsIMutI CAGTG 1 cut(s) 1015
Cac8I GCNNGC 6 cut(s) 7, 132, 350, 494, 931, 1006
CfoI GCGC 2 cut(s) 163, 412
Cfr10I RCCGGY 3 cut(s) 32, 130, 463
Cfr13I GGNCC 5 cut(s) 35, 71, 382, 581, 776
Cfr9I CCCGGG 1 cut(s) 155
CseI GACGC 2 cut(s) 123, 278
Csp6I GTAC 3 cut(s) 55, 103, 256
CviAII CATG 1 cut(s) 341
CviQI GTAC 3 cut(s) 55, 103, 256
DdeI CTNAG 4 cut(s) 150, 283, 498, 585
DinI GGCGCC 1 cut(s) 411
DpnI GATC 2 cut(s) 140, 552
DpnII GATC 2 cut(s) 138, 550
DraI TTTAAA 1 cut(s) 238
DrdI GACNNNNNNGTC 1 cut(s) 1022
DseDI GACNNNNNNGTC 1 cut(s) 1022
EaeI YGGCCR 2 cut(s) 289, 375
Eam1104I CTCTTC 2 cut(s) 15, 117
EarI CTCTTC 2 cut(s) 15, 117
Eco130I CCWWGG 2 cut(s) 15, 174
Eco24I GRGCYC 2 cut(s) 27, 805
Eco47I GGWCC 1 cut(s) 382
Eco57I CTGAAG 2 cut(s) 64, 185
Eco88I CYCGRG 1 cut(s) 155
EcoO109I RGGNCCY 1 cut(s) 581
EcoRI GAATTC 1 cut(s) 197
EcoRII CCWGG 6 cut(s) 352, 505, 577, 612, 688, 804
EcoT14I CCWWGG 2 cut(s) 15, 174
EcoT38I GRGCYC 2 cut(s) 27, 805
EgeI GGCGCC 1 cut(s) 411
EheI GGCGCC 1 cut(s) 411
ErhI CCWWGG 2 cut(s) 15, 174
Esp3I CGTCTC 2 cut(s) 78, 846
FaeI CATG 1 cut(s) 344
FaiI YATR 5 cut(s) 342, 374, 432, 620, 647
FatI CATG 1 cut(s) 340
FauI CCCGC 1 cut(s) 393
FbaI TGATCA 1 cut(s) 550
FriOI GRGCYC 2 cut(s) 27, 805
FspBI CTAG 2 cut(s) 441, 891
GlaI GCGC 2 cut(s) 162, 411
HaeII RGCGCY 2 cut(s) 164, 413
HaeIII GGCC 6 cut(s) 36, 72, 291, 377, 582, 778
HapII CCGG 6 cut(s) 33, 131, 156, 337, 398, 464
HgaI GACGC 2 cut(s) 123, 278
HhaI GCGC 2 cut(s) 163, 412
Hin1I GRCGYC 3 cut(s) 134, 270, 410
Hin1II CATG 1 cut(s) 344
Hin6I GCGC 2 cut(s) 161, 410
HinP1I GCGC 2 cut(s) 161, 410
HincII GTYRAC 3 cut(s) 601, 769, 1027
HindII GTYRAC 3 cut(s) 601, 769, 1027
HinfI GANTC 3 cut(s) 359, 502, 1019
HpaII CCGG 6 cut(s) 33, 131, 156, 337, 398, 464
HphI GGTGA 4 cut(s) 308, 642, 770, 820
Hpy166II GTNNAC 4 cut(s) 382, 601, 769, 1027
Hpy188I TCNGA 6 cut(s) 83, 94, 550, 717, 841, 1037
Hpy188III TCNNGA 1 cut(s) 202
Hpy8I GTNNAC 4 cut(s) 382, 601, 769, 1027
Hpy99I CGWCG 3 cut(s) 139, 272, 461
HpyAV CCTTC 3 cut(s) 88, 806, 863
HpyCH4III ACNGT 1 cut(s) 193
HpyCH4IV ACGT 2 cut(s) 233, 296
HpyCH4V TGCA 5 cut(s) 108, 449, 818, 866, 1004
HpyF3I CTNAG 4 cut(s) 150, 283, 498, 585
HpySE526I ACGT 2 cut(s) 233, 296
Hsp92I GRCGYC 3 cut(s) 134, 270, 410
Hsp92II CATG 1 cut(s) 344
HspAI GCGC 2 cut(s) 161, 410
KasI GGCGCC 1 cut(s) 409
KroI GCCGGC 1 cut(s) 130
KroNI GCCGGC 1 cut(s) 132
Ksp22I TGATCA 1 cut(s) 550
Kzo9I GATC 2 cut(s) 138, 550
LguI GCTCTTC 1 cut(s) 15
LmnI GCTCC 5 cut(s) 95, 243, 326, 339, 957
LweI GCATC 1 cut(s) 433
MaeI CTAG 2 cut(s) 441, 891
MaeII ACGT 2 cut(s) 233, 296
MaeIII GTNAC 2 cut(s) 187, 229
MalI GATC 2 cut(s) 140, 552
MbiI CCGCTC 1 cut(s) 321
MboI GATC 2 cut(s) 138, 550
MboII GAAGA 7 cut(s) 32, 104, 178, 181, 605, 641, 686
MfeI CAATTG 1 cut(s) 46
MhlI GDGCHC 2 cut(s) 27, 805
MluCI AATT 2 cut(s) 46, 197
Mly113I GGCGCC 1 cut(s) 410
MmeI TCCRAC 1 cut(s) 285
MroNI GCCGGC 1 cut(s) 130
MseI TTAA 1 cut(s) 237
MslI CAYNNNNRTG 1 cut(s) 717
MspA1I CMGCKG 2 cut(s) 921, 978
MspI CCGG 6 cut(s) 33, 131, 156, 337, 398, 464
MspR9I CCNGG 8 cut(s) 156, 157, 354, 507, 579, 614, 690, 806
MunI CAATTG 1 cut(s) 46
Mva1269I GAATGC 4 cut(s) 449, 670, 820, 868
MvaI CCWGG 6 cut(s) 354, 507, 579, 614, 690, 806
NaeI GCCGGC 1 cut(s) 132
NarI GGCGCC 1 cut(s) 410
NciI CCSGG 2 cut(s) 156, 157
NdeII GATC 2 cut(s) 138, 550
NgoMIV GCCGGC 1 cut(s) 130
NlaIII CATG 1 cut(s) 344
NlaIV GGNNCC 6 cut(s) 37, 73, 91, 384, 411, 777
NmeAIII GCCGAG 1 cut(s) 48
NmuCI GTSAC 1 cut(s) 229
PciSI GCTCTTC 1 cut(s) 15
PctI GAATGC 4 cut(s) 449, 670, 820, 868
PdiI GCCGGC 1 cut(s) 132
PfeI GAWTC 3 cut(s) 359, 502, 1019
PflMI CCANNNNNTGG 1 cut(s) 51
PfoI TCCNGGA 1 cut(s) 688
Ple19I CGATCG 1 cut(s) 141
PluTI GGCGCC 1 cut(s) 413
Psp6I CCWGG 6 cut(s) 352, 505, 577, 612, 688, 804
PspGI CCWGG 6 cut(s) 352, 505, 577, 612, 688, 804
PspN4I GGNNCC 6 cut(s) 37, 73, 91, 384, 411, 777
PspPI GGNCC 5 cut(s) 35, 71, 382, 581, 776
PvuI CGATCG 1 cut(s) 141
RsaI GTAC 3 cut(s) 56, 104, 257
RsaNI GTAC 3 cut(s) 55, 103, 256
RseI CAYNNNNRTG 1 cut(s) 717
SapI GCTCTTC 1 cut(s) 15
SaqAI TTAA 1 cut(s) 237
Sau3AI GATC 2 cut(s) 138, 550
Sau96I GGNCC 5 cut(s) 35, 71, 382, 581, 776
ScaI AGTACT 1 cut(s) 104
ScrFI CCNGG 8 cut(s) 156, 157, 354, 507, 579, 614, 690, 806
SduI GDGCHC 2 cut(s) 27, 805
SfaNI GCATC 1 cut(s) 433
SfoI GGCGCC 1 cut(s) 411
SinI GGWCC 1 cut(s) 382
SmaI CCCGGG 1 cut(s) 157
SmiMI CAYNNNNRTG 1 cut(s) 717
Sse9I AATT 2 cut(s) 46, 197
SsiI CCGC 8 cut(s) 319, 346, 365, 386, 756, 786, 921, 978
SspDI GGCGCC 1 cut(s) 409
SspMI CTAG 2 cut(s) 441, 891
StyD4I CCNGG 8 cut(s) 154, 155, 352, 505, 577, 612, 688, 804
StyI CCWWGG 2 cut(s) 15, 174
TaaI ACNGT 1 cut(s) 193
TaiI ACGT 2 cut(s) 236, 299
TaqI TCGA 5 cut(s) 115, 137, 141, 195, 799
TasI AATT 2 cut(s) 46, 197
TatI WGTACW 2 cut(s) 102, 255
TauI GCSGC 3 cut(s) 368, 788, 981
TfiI GAWTC 3 cut(s) 359, 502, 1019
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TscAI CASTG 1 cut(s) 1015
TseFI GTSAC 1 cut(s) 229
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 5 cut(s) 606, 693, 735, 777, 827
TspGWI ACGGA 2 cut(s) 313, 493
TspMI CCCGGG 1 cut(s) 155
TspRI CASTG 1 cut(s) 1015
Van91I CCANNNNNTGG 1 cut(s) 51
VpaK11BI GGWCC 1 cut(s) 382
XapI RAATTY 1 cut(s) 197
XmaI CCCGGG 1 cut(s) 155
XspI CTAG 2 cut(s) 441, 891
ZrmI AGTACT 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.