MD13G1035100.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004568
Physical Location & Seq
Forward (+)
2465516 .. 2469335
3820 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1035100.v1.1.491

Sequence Viewer

Length: 1989 bp
ATGGCGGCAATCCCGTACGACATTCGCTGCGCGCTTCTCCTGAATCATCACGCATCGTCGTTATCATCACCATCATCGTCCACGTCATCGCCGTCGTTATCCACCATTCGGTCCCTCAGCCCCACCCTTCTCGGCTCGCGCGCCTCCGCGGCGCGTCGAGTCCTCTCGCTGTCGACCTCGTGCTGGACGACGTCGTTCCGGCTCAGAGCCTTCTCCACCGACCCCGGAGAGGCCGCCGCCGTCAAACTTCCGGAGAAGCCGTCGATATGCACGGCCGATGAGCTCCACTACGTGTCCGTGCCGAACTCCGATTGGAAGCTCGCGCTATGGCGATACCTCCCGTGCCCCAAGGCGCCTCAGAGGAAACATCCATTGCTGCTTTTGTCAGGAGTGGGGACCAACGCCATTGGATATGATCTCTCTCCCGAGTCTTCGTTTGCGCGTTACATGTCTAGGCAAGGATTCGAGACCTGGGTTCTCGAAGTTCGTGGTGCTGGACTGAGCGTGCAAGAATCGGATCGCAAGGAAATTCAGCAATCTGCCCATGAAAGGTCTGAAGAGATGGAAGCTGTTTCTGAGAGTGCAACTGATGCAGCTTTTCCTGCTGCCTCAGCACCTGAGACTCCTGCTCAGGAAGAAAAAAATGAAAATTCAGCTTCTAAGAGTGCACCTGATGCAGCTTTTCCTGTTGCCTCGGCACCTGAGACTCCTGCTCGAGAAGAAAAACATGAGAATTCTTCTTCTAAGAGTGCCACTAATGGAGCATTTTCTGCAGAAAAGCAGTCTGGTGCCTCAGCACCTGAGGTTCCTGCTCAAGGAGTGATTTCAGCTTTCAGTGGAGACGTTACAAATATATTAACTGTAGGGGATGAATCAAAATCAGTGACAAAGTTGACAGAAAATTTTACGCAGCTGTCAGAAAGATTTTCTGGCATTCTCAGTGAAGGTCAATCGACGATCATGGCTGCTAAGTTGCTTGACCAAATTTCAAAACTGTTCGCTGGTTCTGCATTATCTGAGCGTATTAATGAGATAAGCTATAAGATTTCAGGCCTGTTAGAGACAAGACAAAACTCAGCTATTGCTAGCCAGATCAAGGACCTCAGTCAAAGGCTTGTAAATATCATTGAAGAGGGTCAGCGATCAGTTTCACCTCCATTGTTTGACTTGCAAGAGCGTTTTAATTCTACGCTGGAAGATTTCCAGAAACAACTTGAATTGATGGTGAAATATGATTGGGACTTTGACCATTACTTGGAAGAGGATGTACCTGCTGTGATGGACTATATAATGGCAGAAAGTAAGCCAAAGGATGGGAAGTTGTTAGCTGTTGGACACTCGATGGGTGGTATATTGCTTTACGCAATGCTTTCACGATGCGGTTCTGAAGGAAGAGAGTCCCAATTAGCAGCTGTTGTAACAATAGCATCATCACTTGATTACACATCTTCAAAGTCAACTCTCAAACTGCTCATACCCCTTGCTGATCCCGCCCAGGCTCTCAATGTGCCAGCTATTCCTTTAGGAAGTCTACTGGCAGCAACTTATCCTCTCTCAACCCGTCCTCCTTATGTTTTCTCTTGGCTCAATAACTTGATTTCAGCAGAGGACATGATGCATCCGGAGTTGTTGGAAAAGCTTGTTTTGAATAACTTTTGCACCATACCCGCAAAACTTCTATTGCAGTTAACAACAGCTTTTCGGAAGGGTGGATTACGTGACAGGAGCGGTGCCTATTTGTACAAGGATCATATAAACAAAAGTAGTGTCCCTATTTTGGCACTTGCCGGAGACCAGGATCTAATTTGCCCACCTGAAGCGGTTGAAGAAACCGTGAAGCTGATTCCACAGCACCTGGTTACATATAAACTATTCGGACAACCGGGTGGTCCACACTACGCTCATTATGATTTGGTCGGAGGAAAATTGGCAGTGGAGCAGGTCCATCCATGTATAGTCGAATTTCTTTGTCGGCACGACTCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4

Protein Analysis

663

Amino Acids

71.83

Weight (kDa)

5.45

Isoelectric Point (pI)

55.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 417 - 635 1e-06 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 194
Acc36I ACCTGC 2 cut(s) 1279, 1930
AccB1I GGYRCC 4 cut(s) 352, 697, 788, 1730
AccB7I CCANNNNNTGG 2 cut(s) 1255, 1313
AccBSI CCGCTC 1 cut(s) 1728
AccI GTMKAC 2 cut(s) 173, 1531
AccII CGCG 7 cut(s) 32, 139, 141, 149, 154, 323, 442
AccIII TCCGGA 2 cut(s) 250, 1621
AclWI GGATC 4 cut(s) 525, 1481, 1755, 1806
AcoI YGGCCR 1 cut(s) 273
AcsI RAATTY 6 cut(s) 528, 649, 733, 901, 984, 1961
AcuI CTGAAG 3 cut(s) 576, 1407, 1836
AcyI GRCGYC 2 cut(s) 191, 353
AdeI CACNNNGTG 1 cut(s) 292
AfaI GTAC 3 cut(s) 17, 1269, 1742
AfiI CCNNNNNNNGG 9 cut(s) 108, 183, 229, 549, 815, 1096, 1255, 1313, 1777
AflIII ACRYGT 2 cut(s) 291, 447
AgsI TTSAA 6 cut(s) 990, 1130, 1217, 1452, 1648, 1826
AjiI CACGTC 1 cut(s) 84
AjnI CCWGG 4 cut(s) 470, 1494, 1794, 1854
Alw21I GWGCWC 2 cut(s) 285, 670
Alw26I GTCTC 6 cut(s) 461, 614, 698, 834, 1055, 1785
Alw44I GTGCAC 1 cut(s) 666
AlwI GGATC 4 cut(s) 525, 1481, 1755, 1806
AlwNI CAGNNNCTG 3 cut(s) 617, 800, 1855
Ama87I CYCGRG 2 cut(s) 425, 714
Aor13HI TCCGGA 2 cut(s) 250, 1621
AoxI GGCC 3 cut(s) 231, 273, 1051
ApaLI GTGCAC 1 cut(s) 666
ApeKI GCWGC 9 cut(s) 27, 376, 593, 605, 677, 910, 965, 1409, 1538
ApoI RAATTY 6 cut(s) 528, 649, 733, 901, 984, 1961
ArsI GACNNNNNNTTYG 2 cut(s) 1752, 1784
AseI ATTAAT 1 cut(s) 1026
AspLEI GCGC 8 cut(s) 32, 34, 141, 143, 154, 325, 355, 442
AspS9I GGNCC 5 cut(s) 111, 396, 1099, 1889, 1942
AsuC2I CCSGG 2 cut(s) 225, 1884
AsuHPI GGTGA 3 cut(s) 60, 1143, 1237
AsuNHI GCTAGC 1 cut(s) 1085
AvaI CYCGRG 2 cut(s) 425, 714
AvaII GGWCC 5 cut(s) 111, 396, 1099, 1889, 1942
AxyI CCTNAGG 1 cut(s) 801
BaeGI GKGCMC 2 cut(s) 347, 670
BanI GGYRCC 4 cut(s) 352, 697, 788, 1730
BanII GRGCYC 1 cut(s) 285
BarI GAAGNNNNNNTAC 2 cut(s) 1251, 1283
BauI CACGAG 1 cut(s) 178
BbsI GAAGAC 1 cut(s) 423
Bbv12I GWGCWC 2 cut(s) 285, 670
BbvCI CCTCAGC 3 cut(s) 116, 610, 793
BbvI GCAGC 9 cut(s) 14, 363, 592, 605, 689, 922, 952, 1421, 1550
BccI CCATC 7 cut(s) 79, 556, 1216, 1273, 1307, 1336, 1953
BceAI ACGGC 4 cut(s) 76, 224, 244, 288
BciT130I CCWGG 4 cut(s) 472, 1496, 1796, 1856
BcnI CCSGG 2 cut(s) 225, 1884
BcoDI GTCTC 6 cut(s) 461, 614, 698, 834, 1055, 1785
BfaI CTAG 2 cut(s) 453, 1086
BfmI CTRYAG 2 cut(s) 771, 861
BfoI RGCGCY 1 cut(s) 356
BfuAI ACCTGC 2 cut(s) 1279, 1930
BglI GCCNNNNNGGC 1 cut(s) 149
Bme1390I CCNGG 6 cut(s) 225, 472, 1496, 1796, 1856, 1884
Bme18I GGWCC 5 cut(s) 111, 396, 1099, 1889, 1942
BmeT110I CYCGRG 2 cut(s) 425, 714
BmgBI CACGTC 1 cut(s) 84
BmgT120I GGNCC 5 cut(s) 111, 396, 1099, 1889, 1942
BmiI GGNNCC 7 cut(s) 113, 354, 397, 699, 790, 807, 1732
BmrFI CCNGG 6 cut(s) 225, 472, 1496, 1796, 1856, 1884
BmsI GCATC 7 cut(s) 62, 580, 664, 1367, 1436, 1605, 1627
BmtI GCTAGC 1 cut(s) 1089
BoxI GACNNNNGTC 1 cut(s) 1104
BpiI GAAGAC 1 cut(s) 423
Bpu10I CCTNAGC 4 cut(s) 116, 610, 630, 793
BpuEI CTTGAG 1 cut(s) 798
BpuMI CCSGG 2 cut(s) 225, 1884
BsaAI YACGTR 2 cut(s) 292, 1718
BsaHI GRCGYC 2 cut(s) 191, 353
BsaI GGTCTC 2 cut(s) 461, 1785
BsaJI CCNNGG 6 cut(s) 147, 223, 348, 471, 693, 1494
BsaWI WCCGGW 2 cut(s) 250, 1621
BsaXI ACNNNNNCTCC 4 cut(s) 245, 275, 1549, 1579
Bsc4I CCNNNNNNNGG 9 cut(s) 108, 183, 229, 549, 815, 1096, 1255, 1313, 1777
Bse1I ACTGG 1 cut(s) 1539
Bse21I CCTNAGG 1 cut(s) 801
Bse3DI GCAATG 2 cut(s) 371, 1371
BseAI TCCGGA 2 cut(s) 250, 1621
BseBI CCWGG 4 cut(s) 472, 1496, 1796, 1856
BseDI CCNNGG 6 cut(s) 147, 223, 348, 471, 693, 1494
BseGI GGATG 6 cut(s) 367, 874, 1270, 1318, 1618, 1945
BseLI CCNNNNNNNGG 9 cut(s) 108, 183, 229, 549, 815, 1096, 1255, 1313, 1777
BseMI GCAATG 2 cut(s) 371, 1371
BseNI ACTGG 1 cut(s) 1539
BsePI GCGCGC 2 cut(s) 30, 139
BseSI GKGCMC 2 cut(s) 347, 670
BseX3I CGGCCG 1 cut(s) 273
BseXI GCAGC 9 cut(s) 14, 363, 592, 605, 689, 922, 952, 1421, 1550
Bsh1236I CGCG 7 cut(s) 32, 139, 141, 149, 154, 323, 442
Bsh1285I CGRYCG 1 cut(s) 276
BshFI GGCC 3 cut(s) 233, 275, 1053
BshNI GGYRCC 4 cut(s) 352, 697, 788, 1730
BsiEI CGRYCG 1 cut(s) 276
BsiHKAI GWGCWC 2 cut(s) 285, 670
BsiHKCI CYCGRG 2 cut(s) 425, 714
BsiSI CCGG 6 cut(s) 199, 225, 251, 1622, 1788, 1883
BsiWI CGTACG 1 cut(s) 15
BslFI GGGAC 5 cut(s) 97, 409, 1253, 1384, 1754
BslI CCNNNNNNNGG 9 cut(s) 108, 183, 229, 549, 815, 1096, 1255, 1313, 1777
BsmAI GTCTC 6 cut(s) 461, 614, 698, 834, 1055, 1785
BsmBI CGTCTC 1 cut(s) 834
BsmFI GGGAC 5 cut(s) 97, 409, 1253, 1384, 1754
BsmI GAATGC 1 cut(s) 933
BsnI GGCC 3 cut(s) 233, 275, 1053
Bso31I GGTCTC 2 cut(s) 461, 1785
BsoBI CYCGRG 2 cut(s) 425, 714
Bsp1286I GDGCHC 3 cut(s) 285, 347, 670
Bsp13I TCCGGA 2 cut(s) 250, 1621
Bsp1407I TGTACA 1 cut(s) 1740
Bsp143I GATC 8 cut(s) 415, 517, 957, 1092, 1142, 1486, 1747, 1798
BspANI GGCC 3 cut(s) 233, 275, 1053
BspEI TCCGGA 2 cut(s) 250, 1621
BspFNI CGCG 7 cut(s) 32, 139, 141, 149, 154, 323, 442
BspLI GGNNCC 7 cut(s) 113, 354, 397, 699, 790, 807, 1732
BspMAI CTGCAG 1 cut(s) 775
BspMI ACCTGC 2 cut(s) 1279, 1930
BspOI GCTAGC 1 cut(s) 1089
BspPI GGATC 4 cut(s) 525, 1481, 1755, 1806
BspT107I GGYRCC 4 cut(s) 352, 697, 788, 1730
BspTNI GGTCTC 2 cut(s) 461, 1785
BsrBI CCGCTC 1 cut(s) 1728
BsrDI GCAATG 2 cut(s) 371, 1371
BsrGI TGTACA 1 cut(s) 1740
BsrI ACTGG 1 cut(s) 1539
BssECI CCNNGG 6 cut(s) 147, 223, 348, 471, 693, 1494
BssHII GCGCGC 2 cut(s) 30, 139
BssMI GATC 8 cut(s) 415, 517, 957, 1092, 1142, 1486, 1747, 1798
BssNI GRCGYC 2 cut(s) 191, 353
BssSI CACGAG 1 cut(s) 178
BssT1I CCWWGG 1 cut(s) 348
Bst2BI CACGAG 1 cut(s) 178
Bst2UI CCWGG 4 cut(s) 472, 1496, 1796, 1856
Bst4CI ACNGT 3 cut(s) 862, 996, 1834
Bst6I CTCTTC 4 cut(s) 552, 1125, 1254, 1387
BstACI GRCGYC 2 cut(s) 191, 353
BstAPI GCANNNNNTGC 3 cut(s) 590, 674, 770
BstAUI TGTACA 1 cut(s) 1740
BstBAI YACGTR 2 cut(s) 292, 1718
BstC8I GCNNGC 7 cut(s) 32, 137, 141, 321, 506, 1087, 1512
BstDSI CCRYGG 1 cut(s) 147
BstENI CCTNNNNNAGG 1 cut(s) 813
BstF5I GGATG 6 cut(s) 367, 874, 1270, 1318, 1618, 1945
BstFNI CGCG 7 cut(s) 32, 139, 141, 149, 154, 323, 442
BstH2I RGCGCY 1 cut(s) 356
BstHHI GCGC 8 cut(s) 32, 34, 141, 143, 154, 325, 355, 442
BstKTI GATC 8 cut(s) 418, 520, 960, 1095, 1145, 1489, 1750, 1801
BstMAI GTCTC 6 cut(s) 461, 614, 698, 834, 1055, 1785
BstMBI GATC 8 cut(s) 415, 517, 957, 1092, 1142, 1486, 1747, 1798
BstMCI CGRYCG 1 cut(s) 276
BstMWI GCNNNNNNNGC 8 cut(s) 149, 590, 602, 611, 674, 770, 1007, 1490
BstNI CCWGG 4 cut(s) 472, 1496, 1796, 1856
BstNSI RCATGY 1 cut(s) 451
BstPAI GACNNNNGTC 1 cut(s) 1104
BstSCI CCNGG 6 cut(s) 223, 470, 1494, 1794, 1854, 1882
BstSFI CTRYAG 2 cut(s) 771, 861
BstSLI GKGCMC 2 cut(s) 347, 670
BstUI CGCG 7 cut(s) 32, 139, 141, 149, 154, 323, 442
BstV1I GCAGC 9 cut(s) 14, 363, 592, 605, 689, 922, 952, 1421, 1550
BstV2I GAAGAC 1 cut(s) 423
BstX2I RGATCY 1 cut(s) 1798
BstYI RGATCY 1 cut(s) 1798
BstZI CGGCCG 1 cut(s) 273
Bsu36I CCTNAGG 1 cut(s) 801
BsuRI GGCC 3 cut(s) 233, 275, 1053
BtgI CCRYGG 1 cut(s) 147
BtgZI GCGATG 1 cut(s) 72
BtrI CACGTC 1 cut(s) 84
BtsCI GGATG 6 cut(s) 367, 874, 1270, 1318, 1618, 1945
BtsI GCAGTG 1 cut(s) 1938
BtsIMutI CAGTG 4 cut(s) 841, 888, 946, 1938
BveI ACCTGC 2 cut(s) 1279, 1930
Cac8I GCNNGC 7 cut(s) 32, 137, 141, 321, 506, 1087, 1512
CaiI CAGNNNCTG 3 cut(s) 617, 800, 1855
CfoI GCGC 8 cut(s) 32, 34, 141, 143, 154, 325, 355, 442
Cfr13I GGNCC 5 cut(s) 111, 396, 1099, 1889, 1942
Cfr42I CCGCGG 1 cut(s) 150
CseI GACGC 1 cut(s) 143
CsiI ACCWGGT 1 cut(s) 1854
Csp6I GTAC 3 cut(s) 16, 1268, 1741
CviAII CATG 6 cut(s) 448, 545, 728, 961, 1612, 1950
CviQI GTAC 3 cut(s) 16, 1268, 1741
DinI GGCGCC 1 cut(s) 354
DpnI GATC 8 cut(s) 417, 519, 959, 1094, 1144, 1488, 1749, 1800
DpnII GATC 8 cut(s) 415, 517, 957, 1092, 1142, 1486, 1747, 1798
DraIII CACNNNGTG 1 cut(s) 292
EaeI YGGCCR 1 cut(s) 273
EagI CGGCCG 1 cut(s) 273
Eam1104I CTCTTC 4 cut(s) 552, 1125, 1254, 1387
EarI CTCTTC 4 cut(s) 552, 1125, 1254, 1387
Ecl136II GAGCTC 1 cut(s) 283
EclXI CGGCCG 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 348
Eco147I AGGCCT 1 cut(s) 1053
Eco24I GRGCYC 1 cut(s) 285
Eco31I GGTCTC 2 cut(s) 461, 1785
Eco47I GGWCC 5 cut(s) 111, 396, 1099, 1889, 1942
Eco52I CGGCCG 1 cut(s) 273
Eco53kI GAGCTC 1 cut(s) 283
Eco57I CTGAAG 3 cut(s) 576, 1407, 1836
Eco81I CCTNAGG 1 cut(s) 801
Eco88I CYCGRG 2 cut(s) 425, 714
EcoICRI GAGCTC 1 cut(s) 283
EcoNI CCTNNNNNAGG 1 cut(s) 813
EcoO109I RGGNCCY 1 cut(s) 1099
EcoRI GAATTC 1 cut(s) 733
EcoRII CCWGG 4 cut(s) 470, 1494, 1794, 1854
EcoT14I CCWWGG 1 cut(s) 348
EcoT22I ATGCAT 1 cut(s) 1620
EcoT38I GRGCYC 1 cut(s) 285
EgeI GGCGCC 1 cut(s) 354
EheI GGCGCC 1 cut(s) 354
ErhI CCWWGG 1 cut(s) 348
Esp3I CGTCTC 1 cut(s) 834
FaeI CATG 6 cut(s) 451, 548, 731, 964, 1615, 1953
FaqI GGGAC 5 cut(s) 97, 409, 1253, 1384, 1754
FatI CATG 6 cut(s) 447, 544, 727, 960, 1611, 1949
FauI CCCGC 2 cut(s) 1498, 1675
FblI GTMKAC 2 cut(s) 173, 1531
FokI GGATG 6 cut(s) 354, 881, 1277, 1325, 1605, 1932
FriOI GRGCYC 1 cut(s) 285
FspBI CTAG 2 cut(s) 453, 1086
GlaI GCGC 8 cut(s) 31, 33, 140, 142, 153, 324, 354, 441
HaeII RGCGCY 1 cut(s) 356
HaeIII GGCC 3 cut(s) 233, 275, 1053
HapII CCGG 6 cut(s) 199, 225, 251, 1622, 1788, 1883
HgaI GACGC 1 cut(s) 143
HhaI GCGC 8 cut(s) 32, 34, 141, 143, 154, 325, 355, 442
Hin1I GRCGYC 2 cut(s) 191, 353
Hin1II CATG 6 cut(s) 451, 548, 731, 964, 1615, 1953
Hin6I GCGC 8 cut(s) 30, 32, 139, 141, 152, 323, 353, 440
HinP1I GCGC 8 cut(s) 30, 32, 139, 141, 152, 323, 353, 440
HincII GTYRAC 4 cut(s) 174, 894, 1458, 1689
HindII GTYRAC 4 cut(s) 174, 894, 1458, 1689
HindIII AAGCTT 1 cut(s) 1637
HpaI GTTAAC 1 cut(s) 1689
HpaII CCGG 6 cut(s) 199, 225, 251, 1622, 1788, 1883
HphI GGTGA 3 cut(s) 60, 1143, 1237
Hpy166II GTNNAC 8 cut(s) 81, 174, 668, 894, 1458, 1532, 1689, 1892
Hpy8I GTNNAC 8 cut(s) 81, 174, 668, 894, 1458, 1532, 1689, 1892
Hpy99I CGWCG 7 cut(s) 61, 97, 159, 193, 196, 265, 958
HpyAV CCTTC 5 cut(s) 137, 220, 938, 1382, 1699
HpyCH4III ACNGT 3 cut(s) 862, 996, 1834
HpyCH4IV ACGT 5 cut(s) 83, 191, 291, 843, 1717
HpyF10VI GCNNNNNNNGC 8 cut(s) 149, 590, 602, 611, 674, 770, 1007, 1490
HpySE526I ACGT 5 cut(s) 83, 191, 291, 843, 1717
Hsp92I GRCGYC 2 cut(s) 191, 353
Hsp92II CATG 6 cut(s) 451, 548, 731, 964, 1615, 1953
HspAI GCGC 8 cut(s) 30, 32, 139, 141, 152, 323, 353, 440
KasI GGCGCC 1 cut(s) 352
Kpn2I TCCGGA 2 cut(s) 250, 1621
KspAI GTTAAC 1 cut(s) 1689
KspI CCGCGG 1 cut(s) 150
Kzo9I GATC 8 cut(s) 415, 517, 957, 1092, 1142, 1486, 1747, 1798
LmnI GCTCC 4 cut(s) 288, 761, 1725, 1936
Lsp1109I GCAGC 9 cut(s) 14, 363, 592, 605, 689, 922, 952, 1421, 1550
LweI GCATC 7 cut(s) 62, 580, 664, 1367, 1436, 1605, 1627
MabI ACCWGGT 1 cut(s) 1854
MaeI CTAG 2 cut(s) 453, 1086
MaeII ACGT 5 cut(s) 83, 191, 291, 843, 1717
MaeIII GTNAC 6 cut(s) 443, 844, 883, 1417, 1718, 1858
MalI GATC 8 cut(s) 417, 519, 959, 1094, 1144, 1488, 1749, 1800
MbiI CCGCTC 1 cut(s) 1728
MboI GATC 8 cut(s) 415, 517, 957, 1092, 1142, 1486, 1747, 1798
MflI RGATCY 1 cut(s) 1798
MhlI GDGCHC 3 cut(s) 285, 347, 670
Mly113I GGCGCC 1 cut(s) 353
MlyI GAGTC 6 cut(s) 168, 437, 616, 700, 1406, 1973
MmeI TCCRAC 3 cut(s) 1312, 1611, 1897
Mph1103I ATGCAT 1 cut(s) 1620
MroI TCCGGA 2 cut(s) 250, 1621
MseI TTAA 4 cut(s) 857, 1026, 1182, 1688
MspA1I CMGCKG 3 cut(s) 149, 913, 1412
MspI CCGG 6 cut(s) 199, 225, 251, 1622, 1788, 1883
MspR9I CCNGG 6 cut(s) 225, 472, 1496, 1796, 1856, 1884
Mva1269I GAATGC 1 cut(s) 933
MvaI CCWGG 4 cut(s) 472, 1496, 1796, 1856
MvnI CGCG 7 cut(s) 32, 139, 141, 149, 154, 323, 442
MwoI GCNNNNNNNGC 8 cut(s) 149, 590, 602, 611, 674, 770, 1007, 1490
NarI GGCGCC 1 cut(s) 353
NciI CCSGG 2 cut(s) 225, 1884
NdeII GATC 8 cut(s) 415, 517, 957, 1092, 1142, 1486, 1747, 1798
NheI GCTAGC 1 cut(s) 1085
NlaIII CATG 6 cut(s) 451, 548, 731, 964, 1615, 1953
NlaIV GGNNCC 7 cut(s) 113, 354, 397, 699, 790, 807, 1732
NmeAIII GCCGAG 2 cut(s) 111, 674
NmuCI GTSAC 2 cut(s) 883, 1718
NsiI ATGCAT 1 cut(s) 1620
NspI RCATGY 1 cut(s) 451
PaeR7I CTCGAG 1 cut(s) 714
PauI GCGCGC 2 cut(s) 30, 139
PceI AGGCCT 1 cut(s) 1053
PciI ACATGT 1 cut(s) 447
PcsI WCGNNNNNNNCGW 2 cut(s) 89, 185
PctI GAATGC 1 cut(s) 933
PfeI GAWTC 5 cut(s) 43, 462, 512, 872, 1843
Pfl23II CGTACG 1 cut(s) 15
PflFI GACNNNGTC 1 cut(s) 190
PflMI CCANNNNNTGG 2 cut(s) 1255, 1313
PleI GAGTC 6 cut(s) 167, 436, 616, 700, 1405, 1973
PluTI GGCGCC 1 cut(s) 356
PpsI GAGTC 6 cut(s) 167, 436, 616, 700, 1405, 1973
Ppu21I YACGTR 2 cut(s) 292, 1718
PpuMI RGGWCCY 1 cut(s) 1099
PscI ACATGT 1 cut(s) 447
PshAI GACNNNNGTC 1 cut(s) 1104
PshBI ATTAAT 1 cut(s) 1026
Psp124BI GAGCTC 1 cut(s) 285
Psp5II RGGWCCY 1 cut(s) 1099
Psp6I CCWGG 4 cut(s) 470, 1494, 1794, 1854
PspGI CCWGG 4 cut(s) 470, 1494, 1794, 1854
PspLI CGTACG 1 cut(s) 15
PspN4I GGNNCC 7 cut(s) 113, 354, 397, 699, 790, 807, 1732
PspPI GGNCC 5 cut(s) 111, 396, 1099, 1889, 1942
PspPPI RGGWCCY 1 cut(s) 1099
PstI CTGCAG 1 cut(s) 775
PstNI CAGNNNCTG 3 cut(s) 617, 800, 1855
PsuI RGATCY 1 cut(s) 1798
PsyI GACNNNGTC 1 cut(s) 190
PteI GCGCGC 2 cut(s) 30, 139
PvuII CAGCTG 2 cut(s) 913, 1412
RsaI GTAC 3 cut(s) 17, 1269, 1742
RsaNI GTAC 3 cut(s) 16, 1268, 1741
SacI GAGCTC 1 cut(s) 285
SacII CCGCGG 1 cut(s) 150
SalI GTCGAC 1 cut(s) 172
SaqAI TTAA 4 cut(s) 857, 1026, 1182, 1688
Sau3AI GATC 8 cut(s) 415, 517, 957, 1092, 1142, 1486, 1747, 1798
Sau96I GGNCC 5 cut(s) 111, 396, 1099, 1889, 1942
SchI GAGTC 6 cut(s) 168, 437, 616, 700, 1406, 1973
ScrFI CCNGG 6 cut(s) 225, 472, 1496, 1796, 1856, 1884
SduI GDGCHC 3 cut(s) 285, 347, 670
SexAI ACCWGGT 1 cut(s) 1854
SfaNI GCATC 7 cut(s) 62, 580, 664, 1367, 1436, 1605, 1627
SfcI CTRYAG 2 cut(s) 771, 861
SfoI GGCGCC 1 cut(s) 354
Sfr274I CTCGAG 1 cut(s) 714
Sfr303I CCGCGG 1 cut(s) 150
SgrBI CCGCGG 1 cut(s) 150
SinI GGWCC 5 cut(s) 111, 396, 1099, 1889, 1942
SlaI CTCGAG 1 cut(s) 714
SmlI CTYRAG 2 cut(s) 714, 813
SmoI CTYRAG 2 cut(s) 714, 813
SseBI AGGCCT 1 cut(s) 1053
SspDI GGCGCC 1 cut(s) 352
SspMI CTAG 2 cut(s) 453, 1086
SstI GAGCTC 1 cut(s) 285
StuI AGGCCT 1 cut(s) 1053
StyD4I CCNGG 6 cut(s) 223, 470, 1494, 1794, 1854, 1882
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 3 cut(s) 862, 996, 1834
TaiI ACGT 5 cut(s) 86, 194, 294, 846, 1720
TaqI TCGA 9 cut(s) 157, 173, 263, 465, 480, 715, 953, 1340, 1959
TaqII GACCGA 1 cut(s) 99
TatI WGTACW 1 cut(s) 1740
TauI GCSGC 4 cut(s) 8, 152, 236, 239
TfiI GAWTC 5 cut(s) 43, 462, 512, 872, 1843
Tru1I TTAA 4 cut(s) 857, 1026, 1182, 1688
Tru9I TTAA 4 cut(s) 857, 1026, 1182, 1688
TscAI CASTG 4 cut(s) 841, 888, 946, 1938
TseFI GTSAC 2 cut(s) 883, 1718
TseI GCWGC 9 cut(s) 27, 376, 593, 605, 677, 910, 965, 1409, 1538
Tsp45I GTSAC 2 cut(s) 883, 1718
TspDTI ATGAA 3 cut(s) 561, 660, 885
TspGWI ACGGA 1 cut(s) 286
TspRI CASTG 4 cut(s) 841, 888, 946, 1938
Tth111I GACNNNGTC 1 cut(s) 190
Van91I CCANNNNNTGG 2 cut(s) 1255, 1313
VneI GTGCAC 1 cut(s) 666
VpaK11BI GGWCC 5 cut(s) 111, 396, 1099, 1889, 1942
VspI ATTAAT 1 cut(s) 1026
XagI CCTNNNNNAGG 1 cut(s) 813
XapI RAATTY 6 cut(s) 528, 649, 733, 901, 984, 1961
XceI RCATGY 1 cut(s) 451
XhoI CTCGAG 1 cut(s) 714
XmiI GTMKAC 2 cut(s) 173, 1531
XspI CTAG 2 cut(s) 453, 1086
ZraI GACGTC 1 cut(s) 192
Zsp2I ATGCAT 1 cut(s) 1620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.