pycom16g03200

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0004568
Physical Location & Seq
Forward (+)
2018740 .. 2019487
748 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g03200.2

Sequence Viewer

Length: 378 bp
ATGATGCATCCAGAGTTGTTGAAAAAACTCGTTTTGAATAACTTTTGCACCATACCCGCAAAACTTCTATTGCAGTTAACAACAGCTTTTCGGGAGGGTGGATTACGTGACAGGAGCGGTACCTTTTTGTACAAGGACCATTTAAACAAAAGTAGTGTCCCTATTTTGGCAATTGCTGGAGACCAGGATCTAATTTGCCCACCTGAAGCGGTAGAAGAAACTGTGAAGCTGATTCCACAGCACCTGGTTACATATAAACTATTTGGAGAACCCGGTGGTCCACACTACGCTCATTATGATTTGGTCGGAGGCAAATTGGCAGTGGAGCAGGTCTATCCATGTATAGTCGAATTTCTTTGTCAGCACGACTCCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4

Protein Analysis

126

Amino Acids

13.96

Weight (kDa)

6.28

Isoelectric Point (pI)

50.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 319
Acc65I GGTACC 1 cut(s) 119
AccB1I GGYRCC 1 cut(s) 119
AccBSI CCGCTC 1 cut(s) 117
AciI CCGC 3 cut(s) 57, 117, 209
AclWI GGATC 1 cut(s) 195
AcsI RAATTY 1 cut(s) 350
AcuI CTGAAG 1 cut(s) 225
AfaI GTAC 2 cut(s) 121, 131
AfiI CCNNNNNNNGG 1 cut(s) 166
AgsI TTSAA 2 cut(s) 22, 37
AjnI CCWGG 2 cut(s) 183, 243
AluBI AGCT 2 cut(s) 86, 229
AluI AGCT 2 cut(s) 86, 229
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 1 cut(s) 195
AlwNI CAGNNNCTG 1 cut(s) 244
ApoI RAATTY 1 cut(s) 350
ArsI GACNNNNNNTTYG 2 cut(s) 141, 173
Asp718I GGTACC 1 cut(s) 119
AspS9I GGNCC 2 cut(s) 136, 278
AsuC2I CCSGG 1 cut(s) 273
AvaII GGWCC 2 cut(s) 136, 278
BanI GGYRCC 1 cut(s) 119
BciT130I CCWGG 2 cut(s) 185, 245
BcnI CCSGG 1 cut(s) 273
BcoDI GTCTC 1 cut(s) 174
BfuAI ACCTGC 1 cut(s) 319
Bme1390I CCNGG 3 cut(s) 185, 245, 273
Bme18I GGWCC 2 cut(s) 136, 278
BmgT120I GGNCC 2 cut(s) 136, 278
BmiI GGNNCC 1 cut(s) 121
BmrFI CCNGG 3 cut(s) 185, 245, 273
BmsI GCATC 1 cut(s) 16
BpmI CTGGAG 1 cut(s) 198
BpuMI CCSGG 1 cut(s) 273
BsaAI YACGTR 1 cut(s) 107
BsaI GGTCTC 1 cut(s) 174
Bsc4I CCNNNNNNNGG 1 cut(s) 166
BseBI CCWGG 2 cut(s) 185, 245
BseGI GGATG 1 cut(s) 7
BseLI CCNNNNNNNGG 1 cut(s) 166
BshNI GGYRCC 1 cut(s) 119
BsiSI CCGG 1 cut(s) 273
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 1 cut(s) 166
BsmAI GTCTC 1 cut(s) 174
BsmFI GGGAC 1 cut(s) 143
Bso31I GGTCTC 1 cut(s) 174
Bsp1407I TGTACA 1 cut(s) 129
Bsp143I GATC 1 cut(s) 187
BspACI CCGC 3 cut(s) 57, 117, 209
BspLI GGNNCC 1 cut(s) 121
BspMI ACCTGC 1 cut(s) 319
BspPI GGATC 1 cut(s) 195
BspT107I GGYRCC 1 cut(s) 119
BspTNI GGTCTC 1 cut(s) 174
BsrBI CCGCTC 1 cut(s) 117
BsrGI TGTACA 1 cut(s) 129
BssMI GATC 1 cut(s) 187
Bst2UI CCWGG 2 cut(s) 185, 245
Bst4CI ACNGT 1 cut(s) 223
BstAUI TGTACA 1 cut(s) 129
BstBAI YACGTR 1 cut(s) 107
BstF5I GGATG 1 cut(s) 7
BstKTI GATC 1 cut(s) 190
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 1 cut(s) 187
BstNI CCWGG 2 cut(s) 185, 245
BstSCI CCNGG 3 cut(s) 183, 243, 271
BstX2I RGATCY 1 cut(s) 187
BstYI RGATCY 1 cut(s) 187
BtsCI GGATG 1 cut(s) 7
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 1 cut(s) 327
BveI ACCTGC 1 cut(s) 319
CaiI CAGNNNCTG 1 cut(s) 244
Cfr13I GGNCC 2 cut(s) 136, 278
CsiI ACCWGGT 1 cut(s) 243
Csp6I GTAC 2 cut(s) 120, 130
CviAII CATG 1 cut(s) 339
CviJI RGCY 2 cut(s) 86, 229
CviKI_1 RGCY 2 cut(s) 86, 229
CviQI GTAC 2 cut(s) 120, 130
DpnI GATC 1 cut(s) 189
DpnII GATC 1 cut(s) 187
DraI TTTAAA 1 cut(s) 144
Eco31I GGTCTC 1 cut(s) 174
Eco47I GGWCC 2 cut(s) 136, 278
Eco57I CTGAAG 1 cut(s) 225
EcoRII CCWGG 2 cut(s) 183, 243
EcoT22I ATGCAT 1 cut(s) 9
FaeI CATG 1 cut(s) 342
FaiI YATR 6 cut(s) 53, 253, 255, 297, 340, 344
FaqI GGGAC 1 cut(s) 143
FatI CATG 1 cut(s) 338
FauI CCCGC 1 cut(s) 64
GsuI CTGGAG 1 cut(s) 198
HapII CCGG 1 cut(s) 273
Hin1II CATG 1 cut(s) 342
HincII GTYRAC 1 cut(s) 78
HindII GTYRAC 1 cut(s) 78
HinfI GANTC 2 cut(s) 232, 368
HpaI GTTAAC 1 cut(s) 78
HpaII CCGG 1 cut(s) 273
Hpy166II GTNNAC 2 cut(s) 78, 281
Hpy188I TCNGA 1 cut(s) 308
Hpy188III TCNNGA 2 cut(s) 11, 92
Hpy8I GTNNAC 2 cut(s) 78, 281
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4IV ACGT 1 cut(s) 106
HpyCH4V TGCA 3 cut(s) 7, 48, 73
HpySE526I ACGT 1 cut(s) 106
Hsp92II CATG 1 cut(s) 342
KpnI GGTACC 1 cut(s) 123
KspAI GTTAAC 1 cut(s) 78
Kzo9I GATC 1 cut(s) 187
LmnI GCTCC 2 cut(s) 114, 325
LweI GCATC 1 cut(s) 16
MabI ACCWGGT 1 cut(s) 243
MaeII ACGT 1 cut(s) 106
MaeIII GTNAC 2 cut(s) 107, 247
MalI GATC 1 cut(s) 189
MbiI CCGCTC 1 cut(s) 117
MboI GATC 1 cut(s) 187
MboII GAAGA 1 cut(s) 227
MfeI CAATTG 1 cut(s) 171
MflI RGATCY 1 cut(s) 187
MluCI AATT 4 cut(s) 171, 192, 314, 350
MlyI GAGTC 1 cut(s) 362
MmeI TCCRAC 1 cut(s) 286
MnlI CCTC 2 cut(s) 88, 302
Mph1103I ATGCAT 1 cut(s) 9
MseI TTAA 2 cut(s) 77, 143
MspI CCGG 1 cut(s) 273
MspR9I CCNGG 3 cut(s) 185, 245, 273
MunI CAATTG 1 cut(s) 171
MvaI CCWGG 2 cut(s) 185, 245
NciI CCSGG 1 cut(s) 273
NdeII GATC 1 cut(s) 187
NlaIII CATG 1 cut(s) 342
NlaIV GGNNCC 1 cut(s) 121
NmuCI GTSAC 1 cut(s) 107
NsiI ATGCAT 1 cut(s) 9
PfeI GAWTC 1 cut(s) 232
PleI GAGTC 1 cut(s) 362
PpsI GAGTC 1 cut(s) 362
Ppu21I YACGTR 1 cut(s) 107
Psp6I CCWGG 2 cut(s) 183, 243
PspGI CCWGG 2 cut(s) 183, 243
PspN4I GGNNCC 1 cut(s) 121
PspPI GGNCC 2 cut(s) 136, 278
PstNI CAGNNNCTG 1 cut(s) 244
PsuI RGATCY 1 cut(s) 187
RsaI GTAC 2 cut(s) 121, 131
RsaNI GTAC 2 cut(s) 120, 130
SaqAI TTAA 2 cut(s) 77, 143
Sau3AI GATC 1 cut(s) 187
Sau96I GGNCC 2 cut(s) 136, 278
SchI GAGTC 1 cut(s) 362
ScrFI CCNGG 3 cut(s) 185, 245, 273
SetI ASST 7 cut(s) 88, 109, 125, 205, 231, 246, 333
SexAI ACCWGGT 1 cut(s) 243
SfaNI GCATC 1 cut(s) 16
SinI GGWCC 2 cut(s) 136, 278
Sse9I AATT 4 cut(s) 171, 192, 314, 350
SsiI CCGC 3 cut(s) 57, 117, 209
StyD4I CCNGG 3 cut(s) 183, 243, 271
TaaI ACNGT 1 cut(s) 223
TaiI ACGT 1 cut(s) 109
TaqI TCGA 1 cut(s) 348
TasI AATT 4 cut(s) 171, 192, 314, 350
TatI WGTACW 1 cut(s) 129
TfiI GAWTC 1 cut(s) 232
Tru1I TTAA 2 cut(s) 77, 143
Tru9I TTAA 2 cut(s) 77, 143
TscAI CASTG 1 cut(s) 327
TseFI GTSAC 1 cut(s) 107
Tsp45I GTSAC 1 cut(s) 107
TspRI CASTG 1 cut(s) 327
VpaK11BI GGWCC 2 cut(s) 136, 278
XapI RAATTY 1 cut(s) 350
Zsp2I ATGCAT 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.