MD16G1037000.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004568
Physical Location & Seq
Forward (+)
2663328 .. 2667456
4129 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1037000.v1.1.491

Sequence Viewer

Length: 1932 bp
ATGGCGGCAATCCAGTACGACATTCGCTGCGCGCTTCTCCTGAATCATCACGCATCATCGTTATCATCGCCATCATCATCCACGTCACCGCCGTCGTTATCCACGATTCGGTCCCTCTGCCCCACCCTCCTCGGCTCGCGCGCCTCCGCGGCGCGTCGAGTCCTCTCGCTGTCGACCTCGTGCTGGACGACGTCGTTCCGGCTCAGAACCTTCTCCACCGGCGCCGGAGAGGCCGCCGTCGTCAAGCTTCCGGAGAAGCCGCCGATATGCACGGCCGATGAACTCCACTACGTGTCCGTGCCGAACTCCGATTGGAAGCTCGCGCTCTGGCGATACCACCCGTGCCCCAAGGCGCCTCAGAGGAAACATCCATTGCTGCTGTTATCAGGCGTGGGGACCAACGCCATTGGATATGATCTCTCTCCTGAGTCTTCGTTTGCGCGTTACATGTCTAGGCAAGGATTCGAGACATGGGTTCTCGAAGTTCGTGGTGCTGGATTGAGCGTGCAAGAATCAGATCGCAAGGAAATTCAGCAATCTGCCCATGAAATATCTAAAGAGATGGAAGCTGTTTCTGAGAGTGCAACTGATGCAGCTTTTCCTGCAGGAAAGCAGTCAAATGGTTTTCCTGTTGCCTCGGCACCTGAGACTCCTGCTCGAGAAGAAAAACATGATAATTCAGCTTCTAAGAGTGCCACTAATGGATCATTTTCTGCAGAAAAGCAGTCCGGTGCCTCAGCATCTGAGGTTCCTGCTCAAGGAGTGATTTCAGCTTTCAGTGGAGACGTTACAAATATAATAACTGTAGGGGATGAATCAAAATCAGTGACAAAGTCGACAGAAACTTTTACGCAGCTGTCAGAAAGATTTTCTGGCTTTCTCAGTGAAGGTCAATCGAAGATCATGTCTGCTAAGTTGCTTGACCAAATTTCAAAACTGTTCGCTGGTTCTGCATTATCTGAGCGTGTTAATGAGATAAGCTATAAGATTTCAGGCCTGTTAGAGACGAGACAAAACTCAGCTATTGCTAGCCAGATCAAGGACCTCAGTCAAAGGCTTGTAAATATCATTGAAGAAGGTCAGCGATCAGTTTCGCCTCCATTGTTTGACTTGCAAGAGCGTTTTAATTCCACGCTGGAAGATTTCCAGAAACAACTTGAATTGATGGTGAAATATGATTGGGACTTTGACCATTACTTGGAAGAGGATGTACCTGCTGCGATGGAATATATAATGGCAGAGAGTAAGCCAAAGGATGGGAAATTGTTAGCCATTGGACACTCAATGGGTGGTATTTTGCTTTACTCAATGCTTTCACGATGCGGTTCTGAAGGAAGAGAGTCCCAATTGGCAGCTGTTGTAACATTAGCGTCGTCACTTGATTACACATCTTCAAAGTCAATTCTCAAATTGCTCATACCCCTTGCTGATCCCGCCGAGGCTCTCAATGTGCCAGCTATTCCCTTAGGAACACTACTGGCAGCAACTTATCCTCTCTCAAATGGTCCTCCTTATGTTTTCTCTTGGCTTAATAACTTGATTTCAGCAGAGGACATGATGCATCCAGAGTTGTTGAAAAAGCTCGTTTTGAATAACTTTTGCACCGTACCCGCAAAACTTCTATTGCAGTTAACAACAGCTTTTCGGGAGGGTGGACTACGTGACAGGAGCGGTACCTTTTTGTATAAGGACCATTTAAACAAAAGTAGTGTCCCTATTTTGGCAATTGCCGGAGACCAGGATCTAATTTGCCCACCTGAAGCCGTAGAAGAAACTGTGAAGCTGATTCCACAGCACCTGGTTACATATAAACTATTTGGAGAACCCGGTGGTCCACACTACGCTCATTATGATTTGGTCGGAGGCAAATTGGCAGTGGAGCAGGTCTATCCATGTATAATCGAATTTCTTTGTCAGCACGACTCCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Hydrolase_4

Protein Analysis

644

Amino Acids

69.94

Weight (kDa)

5.68

Isoelectric Point (pI)

52.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 399 - 617 6.8e-06 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 194
Acc36I ACCTGC 2 cut(s) 1222, 1873
Acc65I GGTACC 1 cut(s) 1673
AccB1I GGYRCC 5 cut(s) 221, 352, 640, 731, 1673
AccB7I CCANNNNNTGG 2 cut(s) 1198, 1256
AccBSI CCGCTC 1 cut(s) 1671
AccI GTMKAC 2 cut(s) 173, 836
AccII CGCG 7 cut(s) 32, 139, 141, 149, 154, 323, 442
AccIII TCCGGA 1 cut(s) 250
AclWI GGATC 3 cut(s) 712, 1424, 1749
AcoI YGGCCR 1 cut(s) 273
AcsI RAATTY 3 cut(s) 528, 927, 1904
AcuI CTGAAG 2 cut(s) 1350, 1779
AcyI GRCGYC 3 cut(s) 191, 222, 353
AdeI CACNNNGTG 1 cut(s) 292
AfaI GTAC 4 cut(s) 17, 1212, 1608, 1675
AfiI CCNNNNNNNGG 7 cut(s) 108, 183, 758, 1039, 1198, 1256, 1720
AflIII ACRYGT 2 cut(s) 291, 447
AgsI TTSAA 6 cut(s) 933, 1073, 1160, 1395, 1576, 1591
AjiI CACGTC 1 cut(s) 84
AjnI CCWGG 2 cut(s) 1737, 1797
Alw26I GTCTC 6 cut(s) 461, 641, 777, 998, 1003, 1728
AlwI GGATC 3 cut(s) 712, 1424, 1749
AlwNI CAGNNNCTG 2 cut(s) 743, 1798
Ama87I CYCGRG 1 cut(s) 657
Aor13HI TCCGGA 1 cut(s) 250
AoxI GGCC 3 cut(s) 231, 273, 994
ApeKI GCWGC 7 cut(s) 27, 376, 593, 853, 1217, 1352, 1481
ApoI RAATTY 3 cut(s) 528, 927, 1904
ArsI GACNNNNNNTTYG 4 cut(s) 890, 922, 1695, 1727
Asp718I GGTACC 1 cut(s) 1673
AspLEI GCGC 9 cut(s) 32, 34, 141, 143, 154, 224, 325, 355, 442
AspS9I GGNCC 6 cut(s) 111, 396, 1042, 1505, 1690, 1832
AsuC2I CCSGG 1 cut(s) 1827
AsuHPI GGTGA 2 cut(s) 78, 1180
AsuNHI GCTAGC 1 cut(s) 1028
AvaI CYCGRG 1 cut(s) 657
AvaII GGWCC 6 cut(s) 111, 396, 1042, 1505, 1690, 1832
AxyI CCTNAGG 1 cut(s) 1465
BaeGI GKGCMC 1 cut(s) 347
BanI GGYRCC 5 cut(s) 221, 352, 640, 731, 1673
BarI GAAGNNNNNNTAC 2 cut(s) 1194, 1226
BauI CACGAG 1 cut(s) 178
BbsI GAAGAC 1 cut(s) 423
BbvCI CCTCAGC 1 cut(s) 736
BbvI GCAGC 7 cut(s) 14, 363, 605, 865, 1204, 1364, 1493
BccI CCATC 5 cut(s) 79, 556, 1159, 1216, 1250
BceAI ACGGC 4 cut(s) 76, 221, 288, 1748
BciT130I CCWGG 2 cut(s) 1739, 1799
BcnI CCSGG 1 cut(s) 1827
BcoDI GTCTC 6 cut(s) 461, 641, 777, 998, 1003, 1728
BfaI CTAG 2 cut(s) 453, 1029
BfmI CTRYAG 3 cut(s) 603, 714, 804
BfoI RGCGCY 2 cut(s) 225, 356
BfuAI ACCTGC 2 cut(s) 1222, 1873
BglI GCCNNNNNGGC 2 cut(s) 149, 230
Bme1390I CCNGG 3 cut(s) 1739, 1799, 1827
Bme18I GGWCC 6 cut(s) 111, 396, 1042, 1505, 1690, 1832
BmeT110I CYCGRG 1 cut(s) 657
BmgBI CACGTC 1 cut(s) 84
BmgT120I GGNCC 6 cut(s) 111, 396, 1042, 1505, 1690, 1832
BmiI GGNNCC 8 cut(s) 113, 223, 354, 397, 642, 733, 750, 1675
BmrFI CCNGG 3 cut(s) 1739, 1799, 1827
BmsI GCATC 6 cut(s) 62, 580, 749, 1310, 1548, 1570
BmtI GCTAGC 1 cut(s) 1032
BoxI GACNNNNGTC 1 cut(s) 1047
BpiI GAAGAC 1 cut(s) 423
Bpu10I CCTNAGC 1 cut(s) 736
BpuEI CTTGAG 1 cut(s) 741
BpuMI CCSGG 1 cut(s) 1827
BsaAI YACGTR 2 cut(s) 292, 1661
BsaHI GRCGYC 3 cut(s) 191, 222, 353
BsaI GGTCTC 1 cut(s) 1728
BsaJI CCNNGG 5 cut(s) 130, 147, 348, 636, 1437
BsaWI WCCGGW 2 cut(s) 250, 728
Bsc4I CCNNNNNNNGG 7 cut(s) 108, 183, 758, 1039, 1198, 1256, 1720
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 2 cut(s) 13, 1482
Bse21I CCTNAGG 1 cut(s) 1465
Bse3DI GCAATG 1 cut(s) 371
BseAI TCCGGA 1 cut(s) 250
BseBI CCWGG 2 cut(s) 1739, 1799
BseDI CCNNGG 5 cut(s) 130, 147, 348, 636, 1437
BseGI GGATG 6 cut(s) 77, 367, 817, 1213, 1261, 1561
BseLI CCNNNNNNNGG 7 cut(s) 108, 183, 758, 1039, 1198, 1256, 1720
BseMI GCAATG 1 cut(s) 371
BseNI ACTGG 2 cut(s) 13, 1482
BsePI GCGCGC 2 cut(s) 30, 139
BseRI GAGGAG 1 cut(s) 119
BseSI GKGCMC 1 cut(s) 347
BseX3I CGGCCG 1 cut(s) 273
BseXI GCAGC 7 cut(s) 14, 363, 605, 865, 1204, 1364, 1493
Bsh1236I CGCG 7 cut(s) 32, 139, 141, 149, 154, 323, 442
Bsh1285I CGRYCG 1 cut(s) 276
BshFI GGCC 3 cut(s) 233, 275, 996
BshNI GGYRCC 5 cut(s) 221, 352, 640, 731, 1673
BsiEI CGRYCG 1 cut(s) 276
BsiHKCI CYCGRG 1 cut(s) 657
BsiSI CCGG 7 cut(s) 199, 219, 225, 251, 729, 1731, 1827
BslFI GGGAC 5 cut(s) 97, 409, 1196, 1327, 1697
BslI CCNNNNNNNGG 7 cut(s) 108, 183, 758, 1039, 1198, 1256, 1720
BsmAI GTCTC 6 cut(s) 461, 641, 777, 998, 1003, 1728
BsmBI CGTCTC 2 cut(s) 777, 998
BsmFI GGGAC 5 cut(s) 97, 409, 1196, 1327, 1697
BsnI GGCC 3 cut(s) 233, 275, 996
Bso31I GGTCTC 1 cut(s) 1728
BsoBI CYCGRG 1 cut(s) 657
Bsp1286I GDGCHC 1 cut(s) 347
Bsp13I TCCGGA 1 cut(s) 250
Bsp143I GATC 8 cut(s) 415, 517, 704, 900, 1035, 1085, 1429, 1741
BspANI GGCC 3 cut(s) 233, 275, 996
BspEI TCCGGA 1 cut(s) 250
BspFNI CGCG 7 cut(s) 32, 139, 141, 149, 154, 323, 442
BspLI GGNNCC 8 cut(s) 113, 223, 354, 397, 642, 733, 750, 1675
BspMAI CTGCAG 2 cut(s) 607, 718
BspMI ACCTGC 2 cut(s) 1222, 1873
BspOI GCTAGC 1 cut(s) 1032
BspPI GGATC 3 cut(s) 712, 1424, 1749
BspT107I GGYRCC 5 cut(s) 221, 352, 640, 731, 1673
BspTNI GGTCTC 1 cut(s) 1728
BsrBI CCGCTC 1 cut(s) 1671
BsrDI GCAATG 1 cut(s) 371
BsrFI RCCGGY 1 cut(s) 218
BsrI ACTGG 2 cut(s) 13, 1482
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 5 cut(s) 130, 147, 348, 636, 1437
BssHII GCGCGC 2 cut(s) 30, 139
BssMI GATC 8 cut(s) 415, 517, 704, 900, 1035, 1085, 1429, 1741
BssNI GRCGYC 3 cut(s) 191, 222, 353
BssSI CACGAG 1 cut(s) 178
BssT1I CCWWGG 1 cut(s) 348
Bst2BI CACGAG 1 cut(s) 178
Bst2UI CCWGG 2 cut(s) 1739, 1799
Bst4CI ACNGT 4 cut(s) 805, 939, 1606, 1777
Bst6I CTCTTC 2 cut(s) 1197, 1330
BstACI GRCGYC 3 cut(s) 191, 222, 353
BstAPI GCANNNNNTGC 1 cut(s) 590
BstBAI YACGTR 2 cut(s) 292, 1661
BstC8I GCNNGC 7 cut(s) 32, 137, 141, 321, 506, 1030, 1455
BstDSI CCRYGG 1 cut(s) 147
BstENI CCTNNNNNAGG 1 cut(s) 756
BstF5I GGATG 6 cut(s) 77, 367, 817, 1213, 1261, 1561
BstFNI CGCG 7 cut(s) 32, 139, 141, 149, 154, 323, 442
BstH2I RGCGCY 2 cut(s) 225, 356
BstHHI GCGC 9 cut(s) 32, 34, 141, 143, 154, 224, 325, 355, 442
BstKTI GATC 8 cut(s) 418, 520, 707, 903, 1038, 1088, 1432, 1744
BstMAI GTCTC 6 cut(s) 461, 641, 777, 998, 1003, 1728
BstMBI GATC 8 cut(s) 415, 517, 704, 900, 1035, 1085, 1429, 1741
BstMCI CGRYCG 1 cut(s) 276
BstMWI GCNNNNNNNGC 6 cut(s) 149, 230, 590, 602, 950, 1433
BstNI CCWGG 2 cut(s) 1739, 1799
BstNSI RCATGY 1 cut(s) 451
BstPAI GACNNNNGTC 1 cut(s) 1047
BstSCI CCNGG 3 cut(s) 1737, 1797, 1825
BstSFI CTRYAG 3 cut(s) 603, 714, 804
BstSLI GKGCMC 1 cut(s) 347
BstUI CGCG 7 cut(s) 32, 139, 141, 149, 154, 323, 442
BstV1I GCAGC 7 cut(s) 14, 363, 605, 865, 1204, 1364, 1493
BstV2I GAAGAC 1 cut(s) 423
BstX2I RGATCY 1 cut(s) 1741
BstYI RGATCY 1 cut(s) 1741
BstZI CGGCCG 1 cut(s) 273
Bsu36I CCTNAGG 1 cut(s) 1465
BsuRI GGCC 3 cut(s) 233, 275, 996
BtgI CCRYGG 1 cut(s) 147
BtgZI GCGATG 2 cut(s) 51, 1235
BtrI CACGTC 1 cut(s) 84
BtsCI GGATG 6 cut(s) 77, 367, 817, 1213, 1261, 1561
BtsI GCAGTG 1 cut(s) 1881
BtsIMutI CAGTG 4 cut(s) 784, 831, 889, 1881
BveI ACCTGC 2 cut(s) 1222, 1873
Cac8I GCNNGC 7 cut(s) 32, 137, 141, 321, 506, 1030, 1455
CaiI CAGNNNCTG 2 cut(s) 743, 1798
CfoI GCGC 9 cut(s) 32, 34, 141, 143, 154, 224, 325, 355, 442
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 6 cut(s) 111, 396, 1042, 1505, 1690, 1832
Cfr42I CCGCGG 1 cut(s) 150
CseI GACGC 2 cut(s) 143, 1359
CsiI ACCWGGT 1 cut(s) 1797
Csp6I GTAC 4 cut(s) 16, 1211, 1607, 1674
CviAII CATG 7 cut(s) 448, 471, 545, 671, 904, 1555, 1893
CviQI GTAC 4 cut(s) 16, 1211, 1607, 1674
DinI GGCGCC 2 cut(s) 223, 354
DpnI GATC 8 cut(s) 417, 519, 706, 902, 1037, 1087, 1431, 1743
DpnII GATC 8 cut(s) 415, 517, 704, 900, 1035, 1085, 1429, 1741
DraI TTTAAA 1 cut(s) 1698
DraIII CACNNNGTG 1 cut(s) 292
EaeI YGGCCR 1 cut(s) 273
EagI CGGCCG 1 cut(s) 273
Eam1104I CTCTTC 2 cut(s) 1197, 1330
EarI CTCTTC 2 cut(s) 1197, 1330
EclXI CGGCCG 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 348
Eco147I AGGCCT 1 cut(s) 996
Eco31I GGTCTC 1 cut(s) 1728
Eco47I GGWCC 6 cut(s) 111, 396, 1042, 1505, 1690, 1832
Eco52I CGGCCG 1 cut(s) 273
Eco57I CTGAAG 2 cut(s) 1350, 1779
Eco81I CCTNAGG 1 cut(s) 1465
Eco88I CYCGRG 1 cut(s) 657
EcoNI CCTNNNNNAGG 1 cut(s) 756
EcoO109I RGGNCCY 1 cut(s) 1042
EcoRII CCWGG 2 cut(s) 1737, 1797
EcoT14I CCWWGG 1 cut(s) 348
EcoT22I ATGCAT 1 cut(s) 1563
EgeI GGCGCC 2 cut(s) 223, 354
EheI GGCGCC 2 cut(s) 223, 354
ErhI CCWWGG 1 cut(s) 348
Esp3I CGTCTC 2 cut(s) 777, 998
FaeI CATG 7 cut(s) 451, 474, 548, 674, 907, 1558, 1896
FaqI GGGAC 5 cut(s) 97, 409, 1196, 1327, 1697
FatI CATG 7 cut(s) 447, 470, 544, 670, 903, 1554, 1892
FauI CCCGC 2 cut(s) 1441, 1618
FblI GTMKAC 2 cut(s) 173, 836
FokI GGATG 6 cut(s) 64, 354, 824, 1220, 1268, 1548
FspBI CTAG 2 cut(s) 453, 1029
GlaI GCGC 9 cut(s) 31, 33, 140, 142, 153, 223, 324, 354, 441
HaeII RGCGCY 2 cut(s) 225, 356
HaeIII GGCC 3 cut(s) 233, 275, 996
HapII CCGG 7 cut(s) 199, 219, 225, 251, 729, 1731, 1827
HgaI GACGC 2 cut(s) 143, 1359
HhaI GCGC 9 cut(s) 32, 34, 141, 143, 154, 224, 325, 355, 442
Hin1I GRCGYC 3 cut(s) 191, 222, 353
Hin1II CATG 7 cut(s) 451, 474, 548, 674, 907, 1558, 1896
Hin6I GCGC 9 cut(s) 30, 32, 139, 141, 152, 222, 323, 353, 440
HinP1I GCGC 9 cut(s) 30, 32, 139, 141, 152, 222, 323, 353, 440
HincII GTYRAC 3 cut(s) 174, 837, 1632
HindII GTYRAC 3 cut(s) 174, 837, 1632
HindIII AAGCTT 1 cut(s) 245
HpaI GTTAAC 1 cut(s) 1632
HpaII CCGG 7 cut(s) 199, 219, 225, 251, 729, 1731, 1827
HphI GGTGA 2 cut(s) 78, 1180
Hpy166II GTNNAC 5 cut(s) 174, 837, 1632, 1655, 1835
Hpy8I GTNNAC 5 cut(s) 174, 837, 1632, 1655, 1835
Hpy99I CGWCG 6 cut(s) 97, 159, 193, 196, 242, 1375
HpyAV CCTTC 4 cut(s) 220, 881, 1070, 1325
HpyCH4III ACNGT 4 cut(s) 805, 939, 1606, 1777
HpyCH4IV ACGT 5 cut(s) 83, 191, 291, 786, 1660
HpyF10VI GCNNNNNNNGC 6 cut(s) 149, 230, 590, 602, 950, 1433
HpySE526I ACGT 5 cut(s) 83, 191, 291, 786, 1660
Hsp92I GRCGYC 3 cut(s) 191, 222, 353
Hsp92II CATG 7 cut(s) 451, 474, 548, 674, 907, 1558, 1896
HspAI GCGC 9 cut(s) 30, 32, 139, 141, 152, 222, 323, 353, 440
KasI GGCGCC 2 cut(s) 221, 352
Kpn2I TCCGGA 1 cut(s) 250
KpnI GGTACC 1 cut(s) 1677
KspAI GTTAAC 1 cut(s) 1632
KspI CCGCGG 1 cut(s) 150
Kzo9I GATC 8 cut(s) 415, 517, 704, 900, 1035, 1085, 1429, 1741
LmnI GCTCC 2 cut(s) 1668, 1879
Lsp1109I GCAGC 7 cut(s) 14, 363, 605, 865, 1204, 1364, 1493
LweI GCATC 6 cut(s) 62, 580, 749, 1310, 1548, 1570
MabI ACCWGGT 1 cut(s) 1797
MaeI CTAG 2 cut(s) 453, 1029
MaeII ACGT 5 cut(s) 83, 191, 291, 786, 1660
MaeIII GTNAC 8 cut(s) 84, 443, 787, 826, 1360, 1374, 1661, 1801
MalI GATC 8 cut(s) 417, 519, 706, 902, 1037, 1087, 1431, 1743
MbiI CCGCTC 1 cut(s) 1671
MboI GATC 8 cut(s) 415, 517, 704, 900, 1035, 1085, 1429, 1741
MboII GAAGA 9 cut(s) 423, 674, 910, 1085, 1151, 1214, 1347, 1383, 1781
MfeI CAATTG 2 cut(s) 1346, 1725
MflI RGATCY 1 cut(s) 1741
MhlI GDGCHC 1 cut(s) 347
Mly113I GGCGCC 2 cut(s) 222, 353
MlyI GAGTC 5 cut(s) 168, 437, 643, 1349, 1916
MmeI TCCRAC 1 cut(s) 1840
Mph1103I ATGCAT 1 cut(s) 1563
MroI TCCGGA 1 cut(s) 250
MseI TTAA 5 cut(s) 969, 1125, 1530, 1631, 1697
MspA1I CMGCKG 3 cut(s) 149, 856, 1355
MspI CCGG 7 cut(s) 199, 219, 225, 251, 729, 1731, 1827
MspR9I CCNGG 3 cut(s) 1739, 1799, 1827
MunI CAATTG 2 cut(s) 1346, 1725
MvaI CCWGG 2 cut(s) 1739, 1799
MvnI CGCG 7 cut(s) 32, 139, 141, 149, 154, 323, 442
MwoI GCNNNNNNNGC 6 cut(s) 149, 230, 590, 602, 950, 1433
NarI GGCGCC 2 cut(s) 222, 353
NciI CCSGG 1 cut(s) 1827
NdeII GATC 8 cut(s) 415, 517, 704, 900, 1035, 1085, 1429, 1741
NheI GCTAGC 1 cut(s) 1028
NlaIII CATG 7 cut(s) 451, 474, 548, 674, 907, 1558, 1896
NlaIV GGNNCC 8 cut(s) 113, 223, 354, 397, 642, 733, 750, 1675
NmeAIII GCCGAG 3 cut(s) 111, 617, 1462
NmuCI GTSAC 4 cut(s) 84, 826, 1374, 1661
NsiI ATGCAT 1 cut(s) 1563
NspI RCATGY 1 cut(s) 451
PaeR7I CTCGAG 1 cut(s) 657
PauI GCGCGC 2 cut(s) 30, 139
PceI AGGCCT 1 cut(s) 996
PciI ACATGT 1 cut(s) 447
PcsI WCGNNNNNNNCGW 3 cut(s) 89, 101, 185
PfeI GAWTC 6 cut(s) 43, 106, 462, 512, 815, 1786
PflFI GACNNNGTC 2 cut(s) 190, 832
PflMI CCANNNNNTGG 2 cut(s) 1198, 1256
PleI GAGTC 5 cut(s) 167, 436, 643, 1348, 1916
PluTI GGCGCC 2 cut(s) 225, 356
PpsI GAGTC 5 cut(s) 167, 436, 643, 1348, 1916
Ppu21I YACGTR 2 cut(s) 292, 1661
PpuMI RGGWCCY 1 cut(s) 1042
PscI ACATGT 1 cut(s) 447
PshAI GACNNNNGTC 1 cut(s) 1047
Psp5II RGGWCCY 1 cut(s) 1042
Psp6I CCWGG 2 cut(s) 1737, 1797
PspGI CCWGG 2 cut(s) 1737, 1797
PspN4I GGNNCC 8 cut(s) 113, 223, 354, 397, 642, 733, 750, 1675
PspPI GGNCC 6 cut(s) 111, 396, 1042, 1505, 1690, 1832
PspPPI RGGWCCY 1 cut(s) 1042
PstI CTGCAG 2 cut(s) 607, 718
PstNI CAGNNNCTG 2 cut(s) 743, 1798
PsuI RGATCY 1 cut(s) 1741
PsyI GACNNNGTC 2 cut(s) 190, 832
PteI GCGCGC 2 cut(s) 30, 139
PvuII CAGCTG 2 cut(s) 856, 1355
RsaI GTAC 4 cut(s) 17, 1212, 1608, 1675
RsaNI GTAC 4 cut(s) 16, 1211, 1607, 1674
SacII CCGCGG 1 cut(s) 150
SalI GTCGAC 2 cut(s) 172, 835
SaqAI TTAA 5 cut(s) 969, 1125, 1530, 1631, 1697
Sau3AI GATC 8 cut(s) 415, 517, 704, 900, 1035, 1085, 1429, 1741
Sau96I GGNCC 6 cut(s) 111, 396, 1042, 1505, 1690, 1832
SbfI CCTGCAGG 1 cut(s) 607
SchI GAGTC 5 cut(s) 168, 437, 643, 1349, 1916
ScrFI CCNGG 3 cut(s) 1739, 1799, 1827
SdaI CCTGCAGG 1 cut(s) 607
SduI GDGCHC 1 cut(s) 347
SexAI ACCWGGT 1 cut(s) 1797
SfaNI GCATC 6 cut(s) 62, 580, 749, 1310, 1548, 1570
SfcI CTRYAG 3 cut(s) 603, 714, 804
SfoI GGCGCC 2 cut(s) 223, 354
Sfr274I CTCGAG 1 cut(s) 657
Sfr303I CCGCGG 1 cut(s) 150
SgrAI CRCCGGYG 1 cut(s) 218
SgrBI CCGCGG 1 cut(s) 150
SinI GGWCC 6 cut(s) 111, 396, 1042, 1505, 1690, 1832
SlaI CTCGAG 1 cut(s) 657
SmlI CTYRAG 2 cut(s) 657, 756
SmoI CTYRAG 2 cut(s) 657, 756
Sse8387I CCTGCAGG 1 cut(s) 607
SseBI AGGCCT 1 cut(s) 996
SspDI GGCGCC 2 cut(s) 221, 352
SspMI CTAG 2 cut(s) 453, 1029
StuI AGGCCT 1 cut(s) 996
StyD4I CCNGG 3 cut(s) 1737, 1797, 1825
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 4 cut(s) 805, 939, 1606, 1777
TaiI ACGT 5 cut(s) 86, 194, 294, 789, 1663
TaqI TCGA 8 cut(s) 157, 173, 465, 480, 658, 836, 896, 1902
TaqII GACCGA 1 cut(s) 99
TauI GCSGC 4 cut(s) 8, 152, 236, 262
TfiI GAWTC 6 cut(s) 43, 106, 462, 512, 815, 1786
Tru1I TTAA 5 cut(s) 969, 1125, 1530, 1631, 1697
Tru9I TTAA 5 cut(s) 969, 1125, 1530, 1631, 1697
TscAI CASTG 4 cut(s) 784, 831, 889, 1881
TseFI GTSAC 4 cut(s) 84, 826, 1374, 1661
TseI GCWGC 7 cut(s) 27, 376, 593, 853, 1217, 1352, 1481
Tsp45I GTSAC 4 cut(s) 84, 826, 1374, 1661
TspDTI ATGAA 3 cut(s) 294, 561, 828
TspGWI ACGGA 1 cut(s) 286
TspRI CASTG 4 cut(s) 784, 831, 889, 1881
Tth111I GACNNNGTC 2 cut(s) 190, 832
Van91I CCANNNNNTGG 2 cut(s) 1198, 1256
VpaK11BI GGWCC 6 cut(s) 111, 396, 1042, 1505, 1690, 1832
XagI CCTNNNNNAGG 1 cut(s) 756
XapI RAATTY 3 cut(s) 528, 927, 1904
XceI RCATGY 1 cut(s) 451
XhoI CTCGAG 1 cut(s) 657
XmiI GTMKAC 2 cut(s) 173, 836
XspI CTAG 2 cut(s) 453, 1029
ZraI GACGTC 1 cut(s) 192
Zsp2I ATGCAT 1 cut(s) 1563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.