MD13G1036300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015902
Physical Location & Seq
Forward (+)
2527772 .. 2528239
468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1036300.v1.1.491

Sequence Viewer

Length: 468 bp
ATGGGAGTTAGCAGCAGCAACAGACCTCAAATCCAAAAGATGGGGACTTTACAGAAATTCAAGCTCCTCGCCACGCAGTGCGGCGTGGCCCAGAGCCCGACGCGAAGCCCGCGGACGAGCCCGTTGATCCAGTTCCGCCGCCGCAAACCCACCTTGCTGATGCTCCTGACCCGGAGCAGCAGTCGCCGCCGTGACCCGCCTGTTCCGATGCCGCTACCGGAGAAGATTCCGCTTCAGAGGAACAGTTTGAAGGACCTGTTCGTGTCGTCGCCACCATTTGAGTTGAACGACGGGAGAAAGGCGGAGAGTGAGAGAGATAAGCGGGTAGAGTCGGGTAGTTTGTTGGTGGTAGGTAAAGAAATTGGTGGAGTTGGACCGGGCTCCCCTAGGCCGGCGTGGACCGGTTTCCGTTACAAGTCGTTACTGAGAAGGGCTTGGCGCCCTGTGCTCGTCACTATTCCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

156

Amino Acids

17.38

Weight (kDa)

11.59

Isoelectric Point (pI)

83.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015902)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68430
fragaria_vesca FvH4_4g26860
malus_domestica MD13G1036300.v1.1 MD16G1038900.v1.1
prunus_persica Prupe.1G315800_v2.0.a1
pyrus_communis pycom13g03160 pycom16g03330
rosa_chinensis RchiOBHm_Chr4g0434621
rosa_laevigata RLG00000006662
rosa_multiflora Rmu_sc0005783.1_g000003 Rmu_sc0005899.1_g000016
rosa_rugosa Rorug04G0277200
rosa_samantha Rh4BG341300 Rh4CG356100
rosa_wichuraiana Rw4G028900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 438
AccB7I CCANNNNNTGG 1 cut(s) 40
AccII CGCG 2 cut(s) 103, 112
AclWI GGATC 1 cut(s) 121
AcsI RAATTY 1 cut(s) 56
AcuI CTGAAG 1 cut(s) 218
AcyI GRCGYC 1 cut(s) 439
AdeI CACNNNGTG 1 cut(s) 78
AfiI CCNNNNNNNGG 2 cut(s) 40, 391
AgeI ACCGGT 1 cut(s) 401
AgsI TTSAA 3 cut(s) 61, 250, 286
AluBI AGCT 1 cut(s) 64
AluI AGCT 1 cut(s) 64
Alw21I GWGCWC 1 cut(s) 450
AlwI GGATC 1 cut(s) 121
AoxI GGCC 2 cut(s) 87, 389
ApeKI GCWGC 3 cut(s) 12, 15, 177
ApoI RAATTY 1 cut(s) 56
AsiGI ACCGGT 1 cut(s) 401
AspA2I CCTAGG 1 cut(s) 386
AspLEI GCGC 1 cut(s) 441
AspS9I GGNCC 4 cut(s) 88, 253, 374, 399
AsuC2I CCSGG 2 cut(s) 172, 378
AvaII GGWCC 3 cut(s) 253, 374, 399
AvrII CCTAGG 1 cut(s) 386
BanI GGYRCC 1 cut(s) 438
BanII GRGCYC 3 cut(s) 98, 122, 383
Bbv12I GWGCWC 1 cut(s) 450
BbvI GCAGC 3 cut(s) 24, 27, 189
BccI CCATC 1 cut(s) 34
BceAI ACGGC 1 cut(s) 174
BcnI CCSGG 2 cut(s) 172, 378
BfaI CTAG 1 cut(s) 387
BfoI RGCGCY 1 cut(s) 442
BisI GCNGC 8 cut(s) 13, 16, 82, 139, 142, 178, 187, 212
BlnI CCTAGG 1 cut(s) 386
BlsI GCNGC 8 cut(s) 14, 17, 83, 140, 143, 179, 188, 213
Bme1390I CCNGG 2 cut(s) 172, 378
Bme18I GGWCC 3 cut(s) 253, 374, 399
BmgT120I GGNCC 4 cut(s) 88, 253, 374, 399
BmiI GGNNCC 2 cut(s) 382, 440
BmrFI CCNGG 2 cut(s) 172, 378
BmsI GCATC 2 cut(s) 150, 198
BpuMI CCSGG 2 cut(s) 172, 378
BsaHI GRCGYC 1 cut(s) 439
BsaJI CCNNGG 2 cut(s) 110, 386
BsaWI WCCGGW 2 cut(s) 217, 401
BsaXI ACNNNNNCTCC 2 cut(s) 166, 196
Bsc4I CCNNNNNNNGG 2 cut(s) 40, 391
Bse118I RCCGGY 2 cut(s) 391, 401
Bse1I ACTGG 1 cut(s) 130
BseDI CCNNGG 2 cut(s) 110, 386
BseLI CCNNNNNNNGG 2 cut(s) 40, 391
BseMII CTCAG 2 cut(s) 416, 453
BseNI ACTGG 1 cut(s) 130
BseRI GAGGAG 1 cut(s) 56
BseXI GCAGC 3 cut(s) 24, 27, 189
Bsh1236I CGCG 2 cut(s) 103, 112
BshFI GGCC 2 cut(s) 89, 391
BshNI GGYRCC 1 cut(s) 438
BshTI ACCGGT 1 cut(s) 401
BsiHKAI GWGCWC 1 cut(s) 450
BsiSI CCGG 5 cut(s) 172, 218, 377, 392, 402
BslFI GGGAC 1 cut(s) 58
BslI CCNNNNNNNGG 2 cut(s) 40, 391
BsmFI GGGAC 1 cut(s) 58
BsnI GGCC 2 cut(s) 89, 391
Bsp1286I GDGCHC 4 cut(s) 98, 122, 383, 450
Bsp143I GATC 1 cut(s) 126
BspANI GGCC 2 cut(s) 89, 391
BspCNI CTCAG 2 cut(s) 417, 454
BspFNI CGCG 2 cut(s) 103, 112
BspLI GGNNCC 2 cut(s) 382, 440
BspPI GGATC 1 cut(s) 121
BspT107I GGYRCC 1 cut(s) 438
BsrFI RCCGGY 2 cut(s) 391, 401
BsrI ACTGG 1 cut(s) 130
BssAI RCCGGY 2 cut(s) 391, 401
BssECI CCNNGG 2 cut(s) 110, 386
BssMI GATC 1 cut(s) 126
BssNI GRCGYC 1 cut(s) 439
BssT1I CCWWGG 1 cut(s) 386
Bst4CI ACNGT 1 cut(s) 245
BstACI GRCGYC 1 cut(s) 439
BstC8I GCNNGC 2 cut(s) 110, 393
BstDEI CTNAG 2 cut(s) 425, 462
BstDSI CCRYGG 1 cut(s) 110
BstFNI CGCG 2 cut(s) 103, 112
BstH2I RGCGCY 1 cut(s) 442
BstHHI GCGC 1 cut(s) 441
BstKTI GATC 1 cut(s) 129
BstMBI GATC 1 cut(s) 126
BstMWI GCNNNNNNNGC 4 cut(s) 109, 183, 186, 445
BstSCI CCNGG 2 cut(s) 170, 376
BstUI CGCG 2 cut(s) 103, 112
BstV1I GCAGC 3 cut(s) 24, 27, 189
BsuRI GGCC 2 cut(s) 89, 391
BtgI CCRYGG 1 cut(s) 110
BtsI GCAGTG 1 cut(s) 83
BtsIMutI CAGTG 1 cut(s) 83
Cac8I GCNNGC 2 cut(s) 110, 393
CfoI GCGC 1 cut(s) 441
Cfr10I RCCGGY 2 cut(s) 391, 401
Cfr13I GGNCC 4 cut(s) 88, 253, 374, 399
Cfr42I CCGCGG 1 cut(s) 113
CseI GACGC 1 cut(s) 109
CspAI ACCGGT 1 cut(s) 401
CviJI RGCY 8 cut(s) 64, 89, 96, 108, 120, 381, 391, 434
CviKI_1 RGCY 8 cut(s) 64, 89, 96, 108, 120, 381, 391, 434
DdeI CTNAG 2 cut(s) 425, 462
DinI GGCGCC 1 cut(s) 440
DpnI GATC 1 cut(s) 128
DpnII GATC 1 cut(s) 126
DraIII CACNNNGTG 1 cut(s) 78
EciI GGCGGA 2 cut(s) 125, 317
Eco130I CCWWGG 1 cut(s) 386
Eco24I GRGCYC 3 cut(s) 98, 122, 383
Eco47I GGWCC 3 cut(s) 253, 374, 399
Eco57I CTGAAG 1 cut(s) 218
EcoO109I RGGNCCY 1 cut(s) 253
EcoT14I CCWWGG 1 cut(s) 386
EcoT38I GRGCYC 3 cut(s) 98, 122, 383
EgeI GGCGCC 1 cut(s) 440
EheI GGCGCC 1 cut(s) 440
ErhI CCWWGG 1 cut(s) 386
FaqI GGGAC 1 cut(s) 58
FauI CCCGC 3 cut(s) 117, 204, 315
Fnu4HI GCNGC 8 cut(s) 13, 16, 82, 139, 142, 178, 187, 212
FriOI GRGCYC 3 cut(s) 98, 122, 383
Fsp4HI GCNGC 8 cut(s) 13, 16, 82, 139, 142, 178, 187, 212
FspBI CTAG 1 cut(s) 387
GlaI GCGC 1 cut(s) 440
GluI GCNGC 8 cut(s) 13, 16, 82, 139, 142, 178, 187, 212
HaeII RGCGCY 1 cut(s) 442
HaeIII GGCC 2 cut(s) 89, 391
HapII CCGG 5 cut(s) 172, 218, 377, 392, 402
HgaI GACGC 1 cut(s) 109
HhaI GCGC 1 cut(s) 441
Hin1I GRCGYC 1 cut(s) 439
Hin6I GCGC 1 cut(s) 439
HinP1I GCGC 1 cut(s) 439
HinfI GANTC 2 cut(s) 226, 329
HpaII CCGG 5 cut(s) 172, 218, 377, 392, 402
Hpy166II GTNNAC 1 cut(s) 399
Hpy188I TCNGA 2 cut(s) 207, 237
Hpy188III TCNNGA 2 cut(s) 166, 461
Hpy8I GTNNAC 1 cut(s) 399
Hpy99I CGWCG 3 cut(s) 103, 271, 293
HpyAV CCTTC 2 cut(s) 244, 423
HpyCH4III ACNGT 1 cut(s) 245
HpyF10VI GCNNNNNNNGC 4 cut(s) 109, 183, 186, 445
HpyF3I CTNAG 2 cut(s) 425, 462
Hsp92I GRCGYC 1 cut(s) 439
HspAI GCGC 1 cut(s) 439
KasI GGCGCC 1 cut(s) 438
KroI GCCGGC 1 cut(s) 391
KroNI GCCGGC 1 cut(s) 393
KspI CCGCGG 1 cut(s) 113
Kzo9I GATC 1 cut(s) 126
LmnI GCTCC 4 cut(s) 69, 168, 174, 386
Lsp1109I GCAGC 3 cut(s) 24, 27, 189
LweI GCATC 2 cut(s) 150, 198
MaeI CTAG 1 cut(s) 387
MaeIII GTNAC 4 cut(s) 191, 410, 420, 451
MalI GATC 1 cut(s) 128
MboI GATC 1 cut(s) 126
MboII GAAGA 1 cut(s) 235
MhlI GDGCHC 4 cut(s) 98, 122, 383, 450
MluCI AATT 2 cut(s) 56, 360
Mly113I GGCGCC 1 cut(s) 439
MlyI GAGTC 1 cut(s) 338
MmeI TCCRAC 1 cut(s) 352
MnlI CCTC 3 cut(s) 36, 77, 231
MroNI GCCGGC 1 cut(s) 391
MspA1I CMGCKG 1 cut(s) 112
MspI CCGG 5 cut(s) 172, 218, 377, 392, 402
MspR9I CCNGG 2 cut(s) 172, 378
MvnI CGCG 2 cut(s) 103, 112
MwoI GCNNNNNNNGC 4 cut(s) 109, 183, 186, 445
NaeI GCCGGC 1 cut(s) 393
NarI GGCGCC 1 cut(s) 439
NciI CCSGG 2 cut(s) 172, 378
NdeII GATC 1 cut(s) 126
NgoMIV GCCGGC 1 cut(s) 391
NlaIV GGNNCC 2 cut(s) 382, 440
NmuCI GTSAC 2 cut(s) 191, 451
PdiI GCCGGC 1 cut(s) 393
PfeI GAWTC 1 cut(s) 226
PflMI CCANNNNNTGG 1 cut(s) 40
PinAI ACCGGT 1 cut(s) 401
PkrI GCNGC 8 cut(s) 14, 17, 83, 140, 143, 179, 188, 213
PleI GAGTC 1 cut(s) 337
PluTI GGCGCC 1 cut(s) 442
PpsI GAGTC 1 cut(s) 337
PpuMI RGGWCCY 1 cut(s) 253
Psp5II RGGWCCY 1 cut(s) 253
PspN4I GGNNCC 2 cut(s) 382, 440
PspPI GGNCC 4 cut(s) 88, 253, 374, 399
PspPPI RGGWCCY 1 cut(s) 253
SacII CCGCGG 1 cut(s) 113
SatI GCNGC 8 cut(s) 13, 16, 82, 139, 142, 178, 187, 212
Sau3AI GATC 1 cut(s) 126
Sau96I GGNCC 4 cut(s) 88, 253, 374, 399
SchI GAGTC 1 cut(s) 338
ScrFI CCNGG 2 cut(s) 172, 378
SduI GDGCHC 4 cut(s) 98, 122, 383, 450
SetI ASST 5 cut(s) 28, 66, 155, 258, 355
SfaNI GCATC 2 cut(s) 150, 198
SfoI GGCGCC 1 cut(s) 440
Sfr303I CCGCGG 1 cut(s) 113
SgrBI CCGCGG 1 cut(s) 113
SinI GGWCC 3 cut(s) 253, 374, 399
Sse9I AATT 2 cut(s) 56, 360
SspDI GGCGCC 1 cut(s) 438
SspMI CTAG 1 cut(s) 387
StyD4I CCNGG 2 cut(s) 170, 376
StyI CCWWGG 1 cut(s) 386
TaaI ACNGT 1 cut(s) 245
TasI AATT 2 cut(s) 56, 360
TauI GCSGC 5 cut(s) 84, 141, 144, 189, 214
TfiI GAWTC 1 cut(s) 226
TscAI CASTG 1 cut(s) 83
TseFI GTSAC 2 cut(s) 191, 451
TseI GCWGC 3 cut(s) 12, 15, 177
Tsp45I GTSAC 2 cut(s) 191, 451
TspGWI ACGGA 1 cut(s) 398
TspRI CASTG 1 cut(s) 83
Van91I CCANNNNNTGG 1 cut(s) 40
VpaK11BI GGWCC 3 cut(s) 253, 374, 399
XapI RAATTY 1 cut(s) 56
XmaJI CCTAGG 1 cut(s) 386
XspI CTAG 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.