Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0012518

Physical Location & Seq

Genomic Coordinates Forward (+)

3847586 .. 3849880

Gene Structure 2295 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1054800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2232 bp

ATGTCTTTTTCCTCAAACTACTACTGCAACTTACTCAAGCTCTGCAGCCAAGCGGGAAGCCATGCGCAAGCCAAGAAGCTCCATTGCTACATTATCAAAACCGTGACTAATCCCGAAACCTTTCTTCTCAACAATCTCGTTACTACTTACGGCAGATTGGGCAACTTAAGCTATGCCCGCCATGTGTTCGACCAAATCCCTCACCGAACCCTTTTCTCATGGAATGCCATTCTCTCCGTTTACTCGAAATCGGGTCACATTTCTGAGATGCAAGATATCTTCAATCGGATGCCGAGCCGAGATGGGGTGTCTTGGAATTCAGTTATTTCGGGGCATGCGTCATGTGGCTTCGTTGCTGAGGGTGTCAAAGTTTATAGCTTGATGTTAAGGGACGGGCCGGATAATTTGAACCGGATTACTTTCTCCACGATGCTTGTGCTTTCGTCGACTCAGGGATGTGTGAACTTGGGTCGACAGCTTCATGGGCAGATAGTGAAATTTGGGTTTGAATCGTATGTATTTGTTGGTAGTCCTTTGGTGGACATGTACTCGAAAGCGGGGTTAATTTTGGATGCAAAGCGGGTTTTTGATTCAATATCAGAGAGAAATGTGGTTATGTATAATACGCTTATCACCGGGCTTTTACGGAGTGGATTGATCGAAGAATCTGAATGTTTGTTTTGTGAAATGCCTGAAAAAGATTCAATTTCGTGGACCACAATGATTAGCGGGCTTAACCAAAATGGTGCAGGCGGAAAAGCACTTGCTAAGTTTAGAGAAATGAGATTGCAAGGCTTAAGCATGGATCAATATACATTTGGTAGTGTACTGACCGCGTGTGGAAGCCTATCTGCCTTGGAAGAGGGCAAGCAAGTTCATGCGTATATCATTAGGACTGATTTTATAGATAACATCTTTGTTGGTAGTGCTCTTGTTGACATGTATTGCAAATGTAGAATCATAAAATCTGCGGAAACAGTTTTCAAGAGAATGAGCCGCAAGAATGTAGTATCGTGGACTGCATTGCTTGTGGGTTATGGACAAAATGGGTACGGTGAAGAAGCTGTAAGAATTTTTTGTGATATGCAGAGAAGCGGGGTTGAACCAGATGATTTTACCCTGGGAAGCGTAATTAGCTCATGTGCCAACCTAGCAAGCTTAGAAGAAGGTGCCCAGTTCCATGGCCAAGCTCTAGCTTCAGGTTTGATGTCTTTTGTTACAGTTTCAAATGCACTGGTCACATTATATGGTAAATGTGGGAGCATCGAAGACTCCCATAGGCTGTTTGATGAGATGAAAATCAGGGACGAAGTCTCGTGGACGGCCTTGGTTTCAGGGTATGCCCAGTTTGGAAAAGCTTATGAGACAATTGAGTTGTTTGAAAGGATGTTAGCCCATGGTTTGAAACCCGATGGAGTTACTTTCATAGGGGTTCTTTCAGCTTGCAGTAGAGCAGGACTAGTGGAGAAAGGGCATCAGTATTTTGAATCAATGGTAAAAGAACATGGAATTTCACCAATTATGGATCACTACACTTGCATTATTGATCTTCTCAGCCGAGCTGGAAGATTAGAAGAAGCAAAAAGCTTCATAAATAAGATGCCATTCCGTCCTGATGCAATTGGTTGGGCCACCTTGTTGAGCTCGTGTAGACTTCATCATAATATGGAAATTGGTAAATGGGCAGCTGAATCTCTTCTAGAATTAGAGCCCCAGAATGCTGCAAGCTATATCTTGCTCTCAAGCATCTATGCTGCCAAAGGGGAATGGGATGAGGTGGCCAAATTGAGGAGAGGAATGAGGGATAAGGGGGCTAGAAAGGAACCAGGGTGCAGTTGGATCAAATATAAGAACAGAGTGCACATCTTTTCAGCAGATGACCAATCAAGTCCATTTTCGGATCAGATATATGCTAAGCTGGAGGAACTGAATAGAAAAATGATAGAAGAGGGGTATGCGCCAGATATGAGTTCTGTTCTTCATGATGTTGAGGAATCAGAAAAGATGAAGATGCTTAATTACCACAGTGAGAAGCTTGCAATTGCGTTTGGATTGATATTTCTTCCTCCTGCCCTTACCATACGAGTAGTTAAAAACCTTCGAGTGTGTGGGGATTGCCACAATGCCACTAAATACATTTCCAAGATCACCAAGAGAGAGATTCTTGTAAGAGATGCTGTCCGGTACCATCTATTTAAAGACGGAACTTGTTCATGCGGAGATTTCTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

744

Amino Acids

82.99

Weight (kDa)

7.26

Isoelectric Point (pI)

42.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	202 - 233	9e-06	PPR repeat family
PPR	PF01535	205 - 233	3.7e-06	PPR repeat
PPR_2	PF13041	234 - 280	1.1e-07	PPR repeat family
PPR_2	PF13041	334 - 383	1.6e-12	PPR repeat family
PPR	PF01535	337 - 367	1.9e-07	PPR repeat
PPR_2	PF13041	436 - 483	3e-07	PPR repeat family
E_motif	PF20431	553 - 615	2.9e-23	E motif
DYW_deaminase	PF14432	651 - 743	9.5e-41	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012518)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68930 AT1G68930
fragaria_vesca	FvH4_4g28920
malus_domestica	MD13G1054800.v1.1 MD16G1053700.v1.1
prunus_persica	Prupe.1G297100_v2.0.a1
pyrus_communis	pycom13g04860
rosa_chinensis	RchiOBHm_Chr4g0437061
rosa_laevigata	RLG00000006472
rosa_multiflora	Rmu_co8326179.1_g000001 Rmu_sc0008325.1_g000001 Rmu_sc0028518.1_g000001
rosa_roxburghii	Rroxscaffold_5G00378140
rosa_rugosa	Rorug04G0298000
rosa_samantha	Rh4AG352500 Rh4BG361800 Rh4CG376100 Rh4DG356000
rosa_wichuraiana	Rw4G030860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	66
Acc65I	GGTACC	1 cut(s)	2184
AccB1I	GGYRCC	2 cut(s)	1169, 2184
AccI	GTMKAC	3 cut(s)	446, 472, 1651
AccII	CGCG	1 cut(s)	836
AclWI	GGATC	4 cut(s)	813, 1533, 1847, 1908
AcoI	YGGCCR	2 cut(s)	1183, 1779
AcsI	RAATTY	4 cut(s)	316, 497, 1071, 1509
AcuI	CTGAAG	1 cut(s)	1182
AfaI	GTAC	4 cut(s)	548, 828, 1052, 2186
AfiI	CCNNNNNNNGG	2 cut(s)	304, 1788
AflII	CTTAAG	2 cut(s)	166, 796
AflIII	ACRYGT	2 cut(s)	543, 939
AhlI	ACTAGT	1 cut(s)	1459
AjnI	CCWGG	2 cut(s)	1119, 1825
Alw21I	GWGCWC	3 cut(s)	931, 1646, 1863
Alw26I	GTCTC	2 cut(s)	1318, 1358
Alw44I	GTGCAC	1 cut(s)	1859
AlwI	GGATC	4 cut(s)	813, 1533, 1847, 1908
AoxI	GGCC	5 cut(s)	395, 1183, 1323, 1629, 1779
ApaLI	GTGCAC	1 cut(s)	1859
ApeKI	GCWGC	4 cut(s)	45, 1685, 1721, 1754
ApoI	RAATTY	4 cut(s)	316, 497, 1071, 1509
Asp700I	GAANNNNTTC	3 cut(s)	120, 1695, 2209
Asp718I	GGTACC	1 cut(s)	2184
AspLEI	GCGC	2 cut(s)	67, 1960
AspS9I	GGNCC	3 cut(s)	395, 714, 1629
AsuC2I	CCSGG	1 cut(s)	637
AsuHPI	GGTGA	5 cut(s)	194, 625, 1067, 1506, 2140
AvaII	GGWCC	1 cut(s)	714
BaeGI	GKGCMC	2 cut(s)	1174, 1863
BalI	TGGCCA	2 cut(s)	1185, 1781
BanI	GGYRCC	2 cut(s)	1169, 2184
BanII	GRGCYC	2 cut(s)	1646, 1713
BauI	CACGAG	2 cut(s)	1315, 1645
BbsI	GAAGAC	1 cut(s)	1275
Bbv12I	GWGCWC	3 cut(s)	931, 1646, 1863
BbvCI	CCTCAGC	1 cut(s)	357
BbvI	GCAGC	4 cut(s)	57, 1697, 1708, 1741
BccI	CCATC	3 cut(s)	296, 1406, 2196
BceAI	ACGGC	2 cut(s)	166, 1338
BcgI	CGANNNNNNTGC	2 cut(s)	418, 452
BciT130I	CCWGG	2 cut(s)	1121, 1827
BcnI	CCSGG	1 cut(s)	637
BcoDI	GTCTC	2 cut(s)	1318, 1358
BcuI	ACTAGT	1 cut(s)	1459
BfaI	CTAG	5 cut(s)	1151, 1193, 1460, 1700, 1815
BfmI	CTRYAG	1 cut(s)	43
BfrI	CTTAAG	2 cut(s)	166, 796
BisI	GCNGC	5 cut(s)	46, 997, 1686, 1722, 1755
BlpI	GCTNAGC	1 cut(s)	1914
BlsI	GCNGC	5 cut(s)	47, 998, 1687, 1723, 1756
Bme1390I	CCNGG	3 cut(s)	637, 1121, 1827
Bme18I	GGWCC	1 cut(s)	714
BmgT120I	GGNCC	3 cut(s)	395, 714, 1629
BmiI	GGNNCC	3 cut(s)	1171, 1824, 2186
BmrFI	CCNGG	3 cut(s)	637, 1121, 1827
BmrI	ACTGGG	2 cut(s)	1168, 1339
BmuI	ACTGGG	2 cut(s)	1168, 1339
BpiI	GAAGAC	1 cut(s)	1275
BpmI	CTGGAG	1 cut(s)	1940
Bpu10I	CCTNAGC	1 cut(s)	357
Bpu1102I	GCTNAGC	1 cut(s)	1914
BpuEI	CTTGAG	2 cut(s)	20, 1726
BpuMI	CCSGG	1 cut(s)	637
BsaBI	GATNNNNATC	1 cut(s)	1298
BsaJI	CCNNGG	7 cut(s)	855, 1119, 1120, 1180, 1326, 1396, 1826
BsaWI	WCCGGW	2 cut(s)	411, 2181
Bsc4I	CCNNNNNNNGG	2 cut(s)	304, 1788
Bse1I	ACTGG	3 cut(s)	1174, 1239, 1345
Bse3DI	GCAATG	2 cut(s)	82, 1022
Bse8I	GATNNNNATC	1 cut(s)	1298
BseBI	CCWGG	2 cut(s)	1121, 1827
BseDI	CCNNGG	7 cut(s)	855, 1119, 1120, 1180, 1326, 1396, 1826
BseGI	GGATG	5 cut(s)	294, 461, 577, 1392, 1777
BseJI	GATNNNNATC	1 cut(s)	1298
BseLI	CCNNNNNNNGG	2 cut(s)	304, 1788
BseMI	GCAATG	2 cut(s)	82, 1022
BseMII	CTCAG	4 cut(s)	255, 348, 464, 1567
BseNI	ACTGG	3 cut(s)	1174, 1239, 1345
BseRI	GAGGAG	1 cut(s)	1804
BseSI	GKGCMC	2 cut(s)	1174, 1863
BseXI	GCAGC	4 cut(s)	57, 1697, 1708, 1741
BsgI	GTGCAG	2 cut(s)	768, 1852
Bsh1236I	CGCG	1 cut(s)	836
BshFI	GGCC	5 cut(s)	397, 1185, 1325, 1631, 1781
BshNI	GGYRCC	2 cut(s)	1169, 2184
BsiHKAI	GWGCWC	3 cut(s)	931, 1646, 1863
BsiSI	CCGG	4 cut(s)	398, 412, 636, 2182
BslFI	GGGAC	2 cut(s)	404, 1319
BslI	CCNNNNNNNGG	2 cut(s)	304, 1788
BsmAI	GTCTC	2 cut(s)	1318, 1358
BsmFI	GGGAC	2 cut(s)	404, 1319
BsmI	GAATGC	2 cut(s)	229, 1723
BsnI	GGCC	5 cut(s)	397, 1185, 1325, 1631, 1781
Bsp1286I	GDGCHC	5 cut(s)	931, 1174, 1646, 1713, 1863
Bsp143I	GATC	7 cut(s)	657, 805, 1525, 1546, 1839, 1900, 2145
Bsp1720I	GCTNAGC	1 cut(s)	1914
Bsp19I	CCATGG	2 cut(s)	1180, 1396
BspANI	GGCC	5 cut(s)	397, 1185, 1325, 1631, 1781
BspCNI	CTCAG	4 cut(s)	256, 349, 463, 1566
BspFNI	CGCG	1 cut(s)	836
BspHI	TCATGA	1 cut(s)	1981
BspLI	GGNNCC	3 cut(s)	1171, 1824, 2186
BspMAI	CTGCAG	1 cut(s)	47
BspPI	GGATC	4 cut(s)	813, 1533, 1847, 1908
BspT107I	GGYRCC	2 cut(s)	1169, 2184
BspTI	CTTAAG	2 cut(s)	166, 796
BsrDI	GCAATG	2 cut(s)	82, 1022
BsrI	ACTGG	3 cut(s)	1174, 1239, 1345
BssECI	CCNNGG	7 cut(s)	855, 1119, 1120, 1180, 1326, 1396, 1826
BssMI	GATC	7 cut(s)	657, 805, 1525, 1546, 1839, 1900, 2145
BssSI	CACGAG	2 cut(s)	1315, 1645
BssT1I	CCWWGG	4 cut(s)	855, 1180, 1326, 1396
Bst2BI	CACGAG	2 cut(s)	1315, 1645
Bst2UI	CCWGG	2 cut(s)	1121, 1827
Bst4CI	ACNGT	5 cut(s)	103, 979, 1055, 1222, 2027
Bst6I	CTCTTC	3 cut(s)	855, 1701, 1941
BstAFI	CTTAAG	2 cut(s)	166, 796
BstDEI	CTNAG	7 cut(s)	264, 357, 450, 768, 1159, 1553, 1914
BstDSI	CCRYGG	2 cut(s)	1180, 1396
BstF5I	GGATG	5 cut(s)	294, 461, 577, 1392, 1777
BstFNI	CGCG	1 cut(s)	836
BstHHI	GCGC	2 cut(s)	67, 1960
BstKTI	GATC	7 cut(s)	660, 808, 1528, 1549, 1842, 1903, 2148
BstMAI	GTCTC	2 cut(s)	1318, 1358
BstMBI	GATC	7 cut(s)	657, 805, 1525, 1546, 1839, 1900, 2145
BstMWI	GCNNNNNNNGC	6 cut(s)	159, 168, 177, 484, 1134, 1151
BstNI	CCWGG	2 cut(s)	1121, 1827
BstNSI	RCATGY	3 cut(s)	338, 547, 943
BstSCI	CCNGG	3 cut(s)	635, 1119, 1825
BstSFI	CTRYAG	1 cut(s)	43
BstSLI	GKGCMC	2 cut(s)	1174, 1863
BstUI	CGCG	1 cut(s)	836
BstV1I	GCAGC	4 cut(s)	57, 1697, 1708, 1741
BstV2I	GAAGAC	1 cut(s)	1275
BstXI	CCANNNNNNTGG	1 cut(s)	1181
BsuRI	GGCC	5 cut(s)	397, 1185, 1325, 1631, 1781
BtgI	CCRYGG	2 cut(s)	1180, 1396
BtsCI	GGATG	5 cut(s)	294, 461, 577, 1392, 1777
BtsIMutI	CAGTG	2 cut(s)	1232, 2032
CciI	TCATGA	1 cut(s)	1981
CfoI	GCGC	2 cut(s)	67, 1960
Cfr13I	GGNCC	3 cut(s)	395, 714, 1629
CseI	GACGC	1 cut(s)	327
Csp6I	GTAC	4 cut(s)	547, 827, 1051, 2185
CspCI	CAANNNNNGTGG	2 cut(s)	415, 450
CviQI	GTAC	4 cut(s)	547, 827, 1051, 2185
DdeI	CTNAG	7 cut(s)	264, 357, 450, 768, 1159, 1553, 1914
DpnI	GATC	7 cut(s)	659, 807, 1527, 1548, 1841, 1902, 2147
DpnII	GATC	7 cut(s)	657, 805, 1525, 1546, 1839, 1900, 2145
DraI	TTTAAA	1 cut(s)	2197
EaeI	YGGCCR	2 cut(s)	1183, 1779
Eam1104I	CTCTTC	3 cut(s)	855, 1701, 1941
EarI	CTCTTC	3 cut(s)	855, 1701, 1941
EciI	GGCGGA	1 cut(s)	768
Ecl136II	GAGCTC	1 cut(s)	1644
Eco130I	CCWWGG	4 cut(s)	855, 1180, 1326, 1396
Eco24I	GRGCYC	2 cut(s)	1646, 1713
Eco32I	GATATC	1 cut(s)	277
Eco47I	GGWCC	1 cut(s)	714
Eco53kI	GAGCTC	1 cut(s)	1644
Eco57I	CTGAAG	1 cut(s)	1182
EcoICRI	GAGCTC	1 cut(s)	1644
EcoRI	GAATTC	1 cut(s)	316
EcoRII	CCWGG	2 cut(s)	1119, 1825
EcoRV	GATATC	1 cut(s)	277
EcoT14I	CCWWGG	4 cut(s)	855, 1180, 1326, 1396
EcoT38I	GRGCYC	2 cut(s)	1646, 1713
ErhI	CCWWGG	4 cut(s)	855, 1180, 1326, 1396
FaqI	GGGAC	2 cut(s)	404, 1319
FauI	CCCGC	6 cut(s)	46, 185, 550, 573, 722, 1088
FblI	GTMKAC	3 cut(s)	446, 472, 1651
Fnu4HI	GCNGC	5 cut(s)	46, 997, 1686, 1722, 1755
FokI	GGATG	5 cut(s)	301, 468, 584, 1399, 1784
FriOI	GRGCYC	2 cut(s)	1646, 1713
Fsp4HI	GCNGC	5 cut(s)	46, 997, 1686, 1722, 1755
FspBI	CTAG	5 cut(s)	1151, 1193, 1460, 1700, 1815
FspI	TGCGCA	1 cut(s)	66
GlaI	GCGC	2 cut(s)	66, 1959
GluI	GCNGC	5 cut(s)	46, 997, 1686, 1722, 1755
GsuI	CTGGAG	1 cut(s)	1940
HaeIII	GGCC	5 cut(s)	397, 1185, 1325, 1631, 1781
HapII	CCGG	4 cut(s)	398, 412, 636, 2182
HgaI	GACGC	1 cut(s)	327
HhaI	GCGC	2 cut(s)	67, 1960
Hin6I	GCGC	2 cut(s)	65, 1958
HinP1I	GCGC	2 cut(s)	65, 1958
HincII	GTYRAC	3 cut(s)	447, 473, 937
HindII	GTYRAC	3 cut(s)	447, 473, 937
HindIII	AAGCTT	4 cut(s)	1156, 1356, 1585, 2033
HpaII	CCGG	4 cut(s)	398, 412, 636, 2182
HphI	GGTGA	5 cut(s)	194, 625, 1067, 1506, 2140
Hpy188I	TCNGA	7 cut(s)	265, 288, 601, 670, 1900, 1905, 1999
Hpy188III	TCNNGA	5 cut(s)	113, 985, 1613, 1700, 1982
Hpy99I	CGWCG	1 cut(s)	448
HpyAV	CCTTC	2 cut(s)	1160, 2108
HpyCH4III	ACNGT	5 cut(s)	103, 979, 1055, 1222, 2027
HpyF10VI	GCNNNNNNNGC	6 cut(s)	159, 168, 177, 484, 1134, 1151
HpyF3I	CTNAG	7 cut(s)	264, 357, 450, 768, 1159, 1553, 1914
HspAI	GCGC	2 cut(s)	65, 1958
KpnI	GGTACC	1 cut(s)	2188
Kzo9I	GATC	7 cut(s)	657, 805, 1525, 1546, 1839, 1900, 2145
LmnI	GCTCC	2 cut(s)	84, 1260
Lsp1109I	GCAGC	4 cut(s)	57, 1697, 1708, 1741
MaeI	CTAG	5 cut(s)	1151, 1193, 1460, 1700, 1815
MaeIII	GTNAC	6 cut(s)	103, 139, 254, 1216, 1237, 1417
MalI	GATC	7 cut(s)	659, 807, 1527, 1548, 1841, 1902, 2147
MboI	GATC	7 cut(s)	657, 805, 1525, 1546, 1839, 1900, 2145
MfeI	CAATTG	3 cut(s)	1368, 1620, 2040
MhlI	GDGCHC	5 cut(s)	931, 1174, 1646, 1713, 1863
MlsI	TGGCCA	2 cut(s)	1185, 1781
MluNI	TGGCCA	2 cut(s)	1185, 1781
MlyI	GAGTC	2 cut(s)	442, 1265
MmeI	TCCRAC	1 cut(s)	1817
Mox20I	TGGCCA	2 cut(s)	1185, 1781
MroXI	GAANNNNTTC	3 cut(s)	120, 1695, 2209
MscI	TGGCCA	2 cut(s)	1185, 1781
MseI	TTAA	8 cut(s)	167, 386, 563, 735, 797, 2016, 2091, 2196
Msp20I	TGGCCA	2 cut(s)	1185, 1781
MspA1I	CMGCKG	1 cut(s)	1688
MspCI	CTTAAG	2 cut(s)	166, 796
MspI	CCGG	4 cut(s)	398, 412, 636, 2182
MspR9I	CCNGG	3 cut(s)	637, 1121, 1827
MunI	CAATTG	3 cut(s)	1368, 1620, 2040
Mva1269I	GAATGC	2 cut(s)	229, 1723
MvaI	CCWGG	2 cut(s)	1121, 1827
MvnI	CGCG	1 cut(s)	836
MwoI	GCNNNNNNNGC	6 cut(s)	159, 168, 177, 484, 1134, 1151
NciI	CCSGG	1 cut(s)	637
NcoI	CCATGG	2 cut(s)	1180, 1396
NdeII	GATC	7 cut(s)	657, 805, 1525, 1546, 1839, 1900, 2145
NlaIV	GGNNCC	3 cut(s)	1171, 1824, 2186
NmeAIII	GCCGAG	3 cut(s)	318, 323, 1583
NmuCI	GTSAC	3 cut(s)	103, 254, 1237
NsbI	TGCGCA	1 cut(s)	66
NspI	RCATGY	3 cut(s)	338, 547, 943
PaeI	GCATGC	1 cut(s)	338
PagI	TCATGA	1 cut(s)	1981
PasI	CCCWGGG	1 cut(s)	1120
PciI	ACATGT	2 cut(s)	543, 939
PcsI	WCGNNNNNNNCGW	1 cut(s)	335
PctI	GAATGC	2 cut(s)	229, 1723
PdmI	GAANNNNTTC	3 cut(s)	120, 1695, 2209
PfeI	GAWTC	9 cut(s)	509, 590, 665, 701, 957, 1487, 1691, 1994, 2161
PflFI	GACNNNGTC	1 cut(s)	1310
PkrI	GCNGC	5 cut(s)	47, 998, 1687, 1723, 1756
PleI	GAGTC	2 cut(s)	442, 1265
PpsI	GAGTC	2 cut(s)	442, 1265
PscI	ACATGT	2 cut(s)	543, 939
Psp124BI	GAGCTC	1 cut(s)	1646
Psp6I	CCWGG	2 cut(s)	1119, 1825
PspGI	CCWGG	2 cut(s)	1119, 1825
PspN4I	GGNNCC	3 cut(s)	1171, 1824, 2186
PspPI	GGNCC	3 cut(s)	395, 714, 1629
PstI	CTGCAG	1 cut(s)	47
PsyI	GACNNNGTC	1 cut(s)	1310
PvuII	CAGCTG	1 cut(s)	1688
RsaI	GTAC	4 cut(s)	548, 828, 1052, 2186
RsaNI	GTAC	4 cut(s)	547, 827, 1051, 2185
SacI	GAGCTC	1 cut(s)	1646
SalI	GTCGAC	2 cut(s)	445, 471
SaqAI	TTAA	8 cut(s)	167, 386, 563, 735, 797, 2016, 2091, 2196
SatI	GCNGC	5 cut(s)	46, 997, 1686, 1722, 1755
Sau3AI	GATC	7 cut(s)	657, 805, 1525, 1546, 1839, 1900, 2145
Sau96I	GGNCC	3 cut(s)	395, 714, 1629
SchI	GAGTC	2 cut(s)	442, 1265
ScrFI	CCNGG	3 cut(s)	637, 1121, 1827
SduI	GDGCHC	5 cut(s)	931, 1174, 1646, 1713, 1863
SfcI	CTRYAG	1 cut(s)	43
SinI	GGWCC	1 cut(s)	714
SmlI	CTYRAG	4 cut(s)	35, 166, 796, 1741
SmoI	CTYRAG	4 cut(s)	35, 166, 796, 1741
SpeI	ACTAGT	1 cut(s)	1459
SphI	GCATGC	1 cut(s)	338
SspMI	CTAG	5 cut(s)	1151, 1193, 1460, 1700, 1815
SstI	GAGCTC	1 cut(s)	1646
StyD4I	CCNGG	3 cut(s)	635, 1119, 1825
StyI	CCWWGG	4 cut(s)	855, 1180, 1326, 1396
TaaI	ACNGT	5 cut(s)	103, 979, 1055, 1222, 2027
TaqI	TCGA	8 cut(s)	189, 245, 446, 472, 551, 660, 1266, 2101
TatI	WGTACW	2 cut(s)	546, 826
TauI	GCSGC	1 cut(s)	999
TfiI	GAWTC	9 cut(s)	509, 590, 665, 701, 957, 1487, 1691, 1994, 2161
Tru1I	TTAA	8 cut(s)	167, 386, 563, 735, 797, 2016, 2091, 2196
Tru9I	TTAA	8 cut(s)	167, 386, 563, 735, 797, 2016, 2091, 2196
TscAI	CASTG	2 cut(s)	1239, 2032
TseFI	GTSAC	3 cut(s)	103, 254, 1237
TseI	GCWGC	4 cut(s)	45, 1685, 1721, 1754
Tsp45I	GTSAC	3 cut(s)	103, 254, 1237
TspDTI	ATGAA	9 cut(s)	470, 866, 1310, 1414, 1579, 1646, 1970, 2021, 2202
TspGWI	ACGGA	4 cut(s)	226, 661, 1598, 2217
TspRI	CASTG	2 cut(s)	1239, 2032
Tth111I	GACNNNGTC	1 cut(s)	1310
Vha464I	CTTAAG	2 cut(s)	166, 796
VneI	GTGCAC	1 cut(s)	1859
VpaK11BI	GGWCC	1 cut(s)	714
XapI	RAATTY	4 cut(s)	316, 497, 1071, 1509
XbaI	TCTAGA	1 cut(s)	1699
XceI	RCATGY	3 cut(s)	338, 547, 943
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1833
XmiI	GTMKAC	3 cut(s)	446, 472, 1651
XmnI	GAANNNNTTC	3 cut(s)	120, 1695, 2209
XspI	CTAG	5 cut(s)	1151, 1193, 1460, 1700, 1815

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.