Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0012518

Physical Location & Seq

Genomic Coordinates Forward (+)

3806889 .. 3808935

Gene Structure 2047 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1053700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1761 bp

ATGCAAGAGATCTTCAATCGAATGCCAACCCGTGATGGGGTGTCTTGGAATTCACTTATTTCGGGGCATGCGTCATGTGGCTCGGTTGCTGAGGCTGTGAAAGTTTATAGCTTGTTGTTAAGGGACGGGCCGGGTAATTTGAACCGGATTACGTTCTCCACTATGCTTGTGCTTTCGTCGAGTCAGGGGTGTGTGAACTTGGGTCGACAGCTTCACGGGCAGATAGTGAAATTTGGGTTTGAATTATATCTGTTTGTTGGTAGTCCTTTGGAGAAGAATGTGGTTATGTATAATACACTGATCACTGGCCTTTTACGATGTGGATTGATCGCAGAATCTGAATGCTTGTTTAGTGAAATGCCTGAAAAAGATTCAATTTCGTGGACTACAATGATTACCGGACTTAACCAAAACGGTGCAGGTGGAAAAGCGCTTGATAAGTTTAGAGAAATGAGAAGGCTTAAGTATGGATCAATATACCCTTGCAAGAGGGCAAGTAATATCATTAGGACTGATTTTATAGATAACATCTTTGTTGTTAGTGCTCTTGTTGACATGTATTGCAAATGTAGAAGCATAAAAGCTGCAGAAATGGTTTTCAAGAGAATGAACCGCAAGAATGTAGTATCGTGGACTGCACTACTTGTGGGATATGGACAGAATGGGTACAGTGAAGAAGCTGTAAGAATTTTTTGTGATATGCAGAGAAACGGGGTTGAACCAGATGACTTTACGCTGGGAAGTGTAATTAGCTCATGCGCGAACCTAGCAAGCTTAGAAGAGGGCGCCCACATCGAAGACTCCCAATGGCTGTTCGATGAGATGGAGATCACGGACGAAGTCTCCTGGACAGCCTTGGTTTCAGGGTATGCTCAGTTTGGAAAAGCTTATGAGACGTTTGATTTGTTTGAAAGGATGTTGGCCCTTGGTTGGAAACCCGATGGAGTTACTTTCATAGGGGTTCTTTCAGCTTGCAGTAGAGCAGGACTCGTGGAGAAAGGACATCACTACTTTGAATCAATGGTGAAAGAACATGGAATTACACCCATTGTGGATCACTACACTTGCATCATCGACCTTCTCAGCCGGGCTGGAAGATTAGAAGAAGCAAAAAACTTCATAAATAAGATGCCTTTCCGTCCAGATGCAATTGGTTGGGCCACCTTGTTGAGCTCGTGTAGACTTCATCATAATATGGAAATTGGTAAATGGGCAGCTGAATCTCTTCTAGAACTAGAGCCCCAGAATGCTGCAAGCTATATCTTGCTCTCAAGCCTCTATGCTGCCAAGGGGAAATGGGATGAGGTGGCCAAATTAAGGAGAGGAATGAGGGATGAGGGCGCTAGAAAGGAACCGGGATGCAGTTGGATCAAATATAAGAACAGAGTGCACATTTTTTCAGCAGATGACCAGTCAAGTCCATTTTCGGATCAGATATATGCTAAGCTGGAGGAACTAAATTGCAAAATGATAGAAGAGGGGTATGAGCCAGATATGAGTTCTGTTCTTCATGATGTTGAGGAATCGGAAAAGATGAAGTTGCCAAATTACCACAGTGAGAAGCTTGCAATTGCATTTGGATTGATATTTCTTCCTCCTGGCCTTCCCATACGAGCAGTTAAAAACCTTCAATTATGTGGGGATTGTCACAATGCCACTAAATACATTTCCAAGATCACTAAGAGAGAGATACTTGTAAGAGATGCTGTCCGGTACCATCTATTTAAAGATGGAACTTGTTCAAGAGGAGATTTCTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

587

Amino Acids

65.96

Weight (kDa)

6.31

Isoelectric Point (pI)

44.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	206 - 255	8.5e-13	PPR repeat family
PPR	PF01535	209 - 239	1.1e-07	PPR repeat
PPR_2	PF13041	279 - 326	8.2e-07	PPR repeat family
E_motif	PF20431	396 - 458	9.7e-23	E motif
DYW_deaminase	PF14432	494 - 586	6.3e-36	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012518)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68930 AT1G68930
fragaria_vesca	FvH4_4g28920
malus_domestica	MD13G1054800.v1.1 MD16G1053700.v1.1
prunus_persica	Prupe.1G297100_v2.0.a1
pyrus_communis	pycom13g04860
rosa_chinensis	RchiOBHm_Chr4g0437061
rosa_laevigata	RLG00000006472
rosa_multiflora	Rmu_co8326179.1_g000001 Rmu_sc0008325.1_g000001 Rmu_sc0028518.1_g000001
rosa_roxburghii	Rroxscaffold_5G00378140
rosa_rugosa	Rorug04G0298000
rosa_samantha	Rh4AG352500 Rh4BG361800 Rh4CG376100 Rh4DG356000
rosa_wichuraiana	Rw4G030860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	410
Acc36I	ACCTGC	1 cut(s)	410
Acc65I	GGTACC	1 cut(s)	1713
AccB1I	GGYRCC	2 cut(s)	785, 1713
AccI	GTMKAC	2 cut(s)	205, 1180
AccII	CGCG	1 cut(s)	761
AciI	CCGC	1 cut(s)	613
AclWI	GGATC	4 cut(s)	478, 1062, 1376, 1437
AcoI	YGGCCR	1 cut(s)	1308
AcsI	RAATTY	3 cut(s)	49, 230, 687
AcyI	GRCGYC	1 cut(s)	786
AfaI	GTAC	2 cut(s)	668, 1715
AfeI	AGCGCT	1 cut(s)	432
AfiI	CCNNNNNNNGG	4 cut(s)	36, 37, 930, 1317
AflII	CTTAAG	1 cut(s)	461
AflIII	ACRYGT	1 cut(s)	555
AjnI	CCWGG	2 cut(s)	845, 1597
Alw21I	GWGCWC	3 cut(s)	547, 1175, 1392
Alw26I	GTCTC	2 cut(s)	847, 887
Alw44I	GTGCAC	1 cut(s)	1388
AlwI	GGATC	4 cut(s)	478, 1062, 1376, 1437
AlwNI	CAGNNNCTG	1 cut(s)	338
Aor51HI	AGCGCT	1 cut(s)	432
AoxI	GGCC	6 cut(s)	128, 307, 921, 1158, 1308, 1600
ApaLI	GTGCAC	1 cut(s)	1388
ApeKI	GCWGC	4 cut(s)	584, 1214, 1250, 1283
ApoI	RAATTY	3 cut(s)	49, 230, 687
ArsI	GACNNNNNNTTYG	2 cut(s)	886, 918
Asp700I	GAANNNNTTC	2 cut(s)	1224, 1738
Asp718I	GGTACC	1 cut(s)	1713
AspLEI	GCGC	4 cut(s)	433, 761, 788, 1343
AspS9I	GGNCC	3 cut(s)	128, 922, 1158
AsuC2I	CCSGG	3 cut(s)	132, 1088, 1356
AsuHPI	GGTGA	1 cut(s)	1036
BaeGI	GKGCMC	1 cut(s)	1392
BalI	TGGCCA	1 cut(s)	1310
BanI	GGYRCC	2 cut(s)	785, 1713
BanII	GRGCYC	2 cut(s)	1175, 1242
BauI	CACGAG	2 cut(s)	989, 1174
BbsI	GAAGAC	1 cut(s)	804
Bbv12I	GWGCWC	3 cut(s)	547, 1175, 1392
BbvCI	CCTCAGC	1 cut(s)	90
BbvI	GCAGC	4 cut(s)	571, 1226, 1237, 1270
BccI	CCATC	5 cut(s)	29, 817, 935, 1724, 1725
BciT130I	CCWGG	2 cut(s)	847, 1599
BclI	TGATCA	1 cut(s)	300
BcnI	CCSGG	3 cut(s)	132, 1088, 1356
BcoDI	GTCTC	2 cut(s)	847, 887
BfaI	CTAG	4 cut(s)	767, 1229, 1235, 1344
BfmI	CTRYAG	1 cut(s)	585
BfoI	RGCGCY	3 cut(s)	434, 789, 1344
BfrI	CTTAAG	1 cut(s)	461
BfuAI	ACCTGC	1 cut(s)	410
BglII	AGATCT	1 cut(s)	9
BisI	GCNGC	4 cut(s)	585, 1215, 1251, 1284
BlpI	GCTNAGC	1 cut(s)	1443
BlsI	GCNGC	4 cut(s)	586, 1216, 1252, 1285
Bme1390I	CCNGG	5 cut(s)	132, 847, 1088, 1356, 1599
BmgT120I	GGNCC	3 cut(s)	128, 922, 1158
BmiI	GGNNCC	3 cut(s)	787, 1353, 1715
BmrFI	CCNGG	5 cut(s)	132, 847, 1088, 1356, 1599
BmsI	GCATC	5 cut(s)	1077, 1119, 1135, 1349, 1693
BpiI	GAAGAC	1 cut(s)	804
BplI	GAGNNNNNCTC	2 cut(s)	972, 1004
BpmI	CTGGAG	1 cut(s)	1469
Bpu10I	CCTNAGC	1 cut(s)	90
Bpu1102I	GCTNAGC	1 cut(s)	1443
BpuEI	CTTGAG	1 cut(s)	1255
BpuMI	CCSGG	3 cut(s)	132, 1088, 1356
BsaBI	GATNNNNATC	1 cut(s)	827
BsaHI	GRCGYC	1 cut(s)	786
BsaJI	CCNNGG	3 cut(s)	855, 925, 1287
BsaWI	WCCGGW	3 cut(s)	144, 398, 1710
BsaXI	ACNNNNNCTCC	2 cut(s)	827, 857
Bsc4I	CCNNNNNNNGG	4 cut(s)	36, 37, 930, 1317
Bse1I	ACTGG	2 cut(s)	310, 1411
Bse8I	GATNNNNATC	1 cut(s)	827
BseBI	CCWGG	2 cut(s)	847, 1599
BseDI	CCNNGG	3 cut(s)	855, 925, 1287
BseGI	GGATG	4 cut(s)	921, 1306, 1339, 1364
BseJI	GATNNNNATC	1 cut(s)	827
BseLI	CCNNNNNNNGG	4 cut(s)	36, 37, 930, 1317
BseMII	CTCAG	3 cut(s)	81, 887, 1096
BseNI	ACTGG	2 cut(s)	310, 1411
BseRI	GAGGAG	1 cut(s)	1761
BseSI	GKGCMC	1 cut(s)	1392
BseXI	GCAGC	4 cut(s)	571, 1226, 1237, 1270
BseYI	CCCAGC	1 cut(s)	736
BsgI	GTGCAG	2 cut(s)	438, 621
Bsh1236I	CGCG	1 cut(s)	761
BshFI	GGCC	6 cut(s)	130, 309, 923, 1160, 1310, 1602
BshNI	GGYRCC	2 cut(s)	785, 1713
BsiHKAI	GWGCWC	3 cut(s)	547, 1175, 1392
BsiSI	CCGG	6 cut(s)	131, 145, 399, 1087, 1355, 1711
BslFI	GGGAC	1 cut(s)	137
BslI	CCNNNNNNNGG	4 cut(s)	36, 37, 930, 1317
BsmAI	GTCTC	2 cut(s)	847, 887
BsmBI	CGTCTC	1 cut(s)	887
BsmFI	GGGAC	1 cut(s)	137
BsmI	GAATGC	3 cut(s)	27, 347, 1252
BsnI	GGCC	6 cut(s)	130, 309, 923, 1160, 1310, 1602
Bsp1286I	GDGCHC	4 cut(s)	547, 1175, 1242, 1392
Bsp143I	GATC	9 cut(s)	9, 300, 327, 470, 828, 1054, 1368, 1429, 1674
Bsp1720I	GCTNAGC	1 cut(s)	1443
BspACI	CCGC	1 cut(s)	613
BspANI	GGCC	6 cut(s)	130, 309, 923, 1160, 1310, 1602
BspCNI	CTCAG	3 cut(s)	82, 886, 1095
BspFNI	CGCG	1 cut(s)	761
BspHI	TCATGA	1 cut(s)	1510
BspLI	GGNNCC	3 cut(s)	787, 1353, 1715
BspMAI	CTGCAG	1 cut(s)	589
BspMI	ACCTGC	1 cut(s)	410
BspPI	GGATC	4 cut(s)	478, 1062, 1376, 1437
BspT107I	GGYRCC	2 cut(s)	785, 1713
BspTI	CTTAAG	1 cut(s)	461
BsrI	ACTGG	2 cut(s)	310, 1411
BssECI	CCNNGG	3 cut(s)	855, 925, 1287
BssMI	GATC	9 cut(s)	9, 300, 327, 470, 828, 1054, 1368, 1429, 1674
BssNI	GRCGYC	1 cut(s)	786
BssSI	CACGAG	2 cut(s)	989, 1174
BssT1I	CCWWGG	3 cut(s)	855, 925, 1287
Bst2BI	CACGAG	2 cut(s)	989, 1174
Bst2UI	CCWGG	2 cut(s)	847, 1599
Bst4CI	ACNGT	3 cut(s)	416, 671, 1556
Bst6I	CTCTTC	3 cut(s)	774, 1230, 1470
BstACI	GRCGYC	1 cut(s)	786
BstAFI	CTTAAG	1 cut(s)	461
BstC8I	GCNNGC	5 cut(s)	69, 772, 973, 1255, 1566
BstDEI	CTNAG	6 cut(s)	90, 775, 873, 1082, 1443, 1680
BstF5I	GGATG	4 cut(s)	921, 1306, 1339, 1364
BstFNI	CGCG	1 cut(s)	761
BstH2I	RGCGCY	3 cut(s)	434, 789, 1344
BstHHI	GCGC	4 cut(s)	433, 761, 788, 1343
BstKTI	GATC	9 cut(s)	12, 303, 330, 473, 831, 1057, 1371, 1432, 1677
BstMAI	GTCTC	2 cut(s)	847, 887
BstMBI	GATC	9 cut(s)	9, 300, 327, 470, 828, 1054, 1368, 1429, 1674
BstMWI	GCNNNNNNNGC	2 cut(s)	217, 767
BstNI	CCWGG	2 cut(s)	847, 1599
BstNSI	RCATGY	2 cut(s)	71, 559
BstSCI	CCNGG	5 cut(s)	130, 845, 1086, 1354, 1597
BstSFI	CTRYAG	1 cut(s)	585
BstSLI	GKGCMC	1 cut(s)	1392
BstUI	CGCG	1 cut(s)	761
BstV1I	GCAGC	4 cut(s)	571, 1226, 1237, 1270
BstV2I	GAAGAC	1 cut(s)	804
BstX2I	RGATCY	1 cut(s)	9
BstYI	RGATCY	1 cut(s)	9
BsuRI	GGCC	6 cut(s)	130, 309, 923, 1160, 1310, 1602
BtsCI	GGATG	4 cut(s)	921, 1306, 1339, 1364
BtsIMutI	CAGTG	4 cut(s)	296, 303, 676, 1561
BveI	ACCTGC	1 cut(s)	410
Cac8I	GCNNGC	5 cut(s)	69, 772, 973, 1255, 1566
CaiI	CAGNNNCTG	1 cut(s)	338
CciI	TCATGA	1 cut(s)	1510
CfoI	GCGC	4 cut(s)	433, 761, 788, 1343
Cfr13I	GGNCC	3 cut(s)	128, 922, 1158
CseI	GACGC	1 cut(s)	60
Csp6I	GTAC	2 cut(s)	667, 1714
CspCI	CAANNNNNGTGG	2 cut(s)	148, 183
CviAII	CATG	6 cut(s)	68, 75, 556, 756, 1034, 1511
CviQI	GTAC	2 cut(s)	667, 1714
DdeI	CTNAG	6 cut(s)	90, 775, 873, 1082, 1443, 1680
DinI	GGCGCC	1 cut(s)	787
DpnI	GATC	9 cut(s)	11, 302, 329, 472, 830, 1056, 1370, 1431, 1676
DpnII	GATC	9 cut(s)	9, 300, 327, 470, 828, 1054, 1368, 1429, 1674
DraI	TTTAAA	1 cut(s)	1726
EaeI	YGGCCR	1 cut(s)	1308
Eam1104I	CTCTTC	3 cut(s)	774, 1230, 1470
EarI	CTCTTC	3 cut(s)	774, 1230, 1470
Ecl136II	GAGCTC	1 cut(s)	1173
Eco130I	CCWWGG	3 cut(s)	855, 925, 1287
Eco24I	GRGCYC	2 cut(s)	1175, 1242
Eco47III	AGCGCT	1 cut(s)	432
Eco53kI	GAGCTC	1 cut(s)	1173
EcoICRI	GAGCTC	1 cut(s)	1173
EcoRI	GAATTC	1 cut(s)	49
EcoRII	CCWGG	2 cut(s)	845, 1597
EcoT14I	CCWWGG	3 cut(s)	855, 925, 1287
EcoT38I	GRGCYC	2 cut(s)	1175, 1242
EgeI	GGCGCC	1 cut(s)	787
EheI	GGCGCC	1 cut(s)	787
ErhI	CCWWGG	3 cut(s)	855, 925, 1287
Esp3I	CGTCTC	1 cut(s)	887
FaeI	CATG	6 cut(s)	71, 78, 559, 759, 1037, 1514
FaqI	GGGAC	1 cut(s)	137
FatI	CATG	6 cut(s)	67, 74, 555, 755, 1033, 1510
FbaI	TGATCA	1 cut(s)	300
FblI	GTMKAC	2 cut(s)	205, 1180
Fnu4HI	GCNGC	4 cut(s)	585, 1215, 1251, 1284
FokI	GGATG	4 cut(s)	928, 1313, 1346, 1371
FriOI	GRGCYC	2 cut(s)	1175, 1242
Fsp4HI	GCNGC	4 cut(s)	585, 1215, 1251, 1284
FspBI	CTAG	4 cut(s)	767, 1229, 1235, 1344
GlaI	GCGC	4 cut(s)	432, 760, 787, 1342
GluI	GCNGC	4 cut(s)	585, 1215, 1251, 1284
GsaI	CCCAGC	1 cut(s)	740
GsuI	CTGGAG	1 cut(s)	1469
HaeII	RGCGCY	3 cut(s)	434, 789, 1344
HaeIII	GGCC	6 cut(s)	130, 309, 923, 1160, 1310, 1602
HapII	CCGG	6 cut(s)	131, 145, 399, 1087, 1355, 1711
HgaI	GACGC	1 cut(s)	60
HhaI	GCGC	4 cut(s)	433, 761, 788, 1343
Hin1I	GRCGYC	1 cut(s)	786
Hin1II	CATG	6 cut(s)	71, 78, 559, 759, 1037, 1514
Hin6I	GCGC	4 cut(s)	431, 759, 786, 1341
HinP1I	GCGC	4 cut(s)	431, 759, 786, 1341
HincII	GTYRAC	2 cut(s)	206, 553
HindII	GTYRAC	2 cut(s)	206, 553
HindIII	AAGCTT	3 cut(s)	772, 885, 1562
HinfI	GANTC	8 cut(s)	181, 335, 371, 800, 987, 1016, 1220, 1523
HpaII	CCGG	6 cut(s)	131, 145, 399, 1087, 1355, 1711
HphI	GGTGA	1 cut(s)	1036
Hpy166II	GTNNAC	7 cut(s)	196, 206, 384, 553, 633, 1181, 1390
Hpy188I	TCNGA	4 cut(s)	340, 1429, 1434, 1528
Hpy188III	TCNNGA	5 cut(s)	601, 1142, 1229, 1511, 1743
Hpy8I	GTNNAC	7 cut(s)	196, 206, 384, 553, 633, 1181, 1390
Hpy99I	CGWCG	1 cut(s)	181
HpyAV	CCTTC	4 cut(s)	450, 1088, 1613, 1637
HpyCH4III	ACNGT	3 cut(s)	416, 671, 1556
HpyCH4IV	ACGT	2 cut(s)	152, 896
HpyF10VI	GCNNNNNNNGC	2 cut(s)	217, 767
HpyF3I	CTNAG	6 cut(s)	90, 775, 873, 1082, 1443, 1680
HpySE526I	ACGT	2 cut(s)	152, 896
Hsp92I	GRCGYC	1 cut(s)	786
Hsp92II	CATG	6 cut(s)	71, 78, 559, 759, 1037, 1514
HspAI	GCGC	4 cut(s)	431, 759, 786, 1341
KasI	GGCGCC	1 cut(s)	785
KpnI	GGTACC	1 cut(s)	1717
Ksp22I	TGATCA	1 cut(s)	300
Kzo9I	GATC	9 cut(s)	9, 300, 327, 470, 828, 1054, 1368, 1429, 1674
Lsp1109I	GCAGC	4 cut(s)	571, 1226, 1237, 1270
LweI	GCATC	5 cut(s)	1077, 1119, 1135, 1349, 1693
MaeI	CTAG	4 cut(s)	767, 1229, 1235, 1344
MaeII	ACGT	2 cut(s)	152, 896
MaeIII	GTNAC	2 cut(s)	946, 1646
MalI	GATC	9 cut(s)	11, 302, 329, 472, 830, 1056, 1370, 1431, 1676
MboI	GATC	9 cut(s)	9, 300, 327, 470, 828, 1054, 1368, 1429, 1674
MfeI	CAATTG	2 cut(s)	1149, 1569
MflI	RGATCY	1 cut(s)	9
MhlI	GDGCHC	4 cut(s)	547, 1175, 1242, 1392
MlsI	TGGCCA	1 cut(s)	1310
MluNI	TGGCCA	1 cut(s)	1310
Mly113I	GGCGCC	1 cut(s)	786
MlyI	GAGTC	3 cut(s)	190, 794, 981
MmeI	TCCRAC	2 cut(s)	911, 1346
Mox20I	TGGCCA	1 cut(s)	1310
MroXI	GAANNNNTTC	2 cut(s)	1224, 1738
MscI	TGGCCA	1 cut(s)	1310
MseI	TTAA	6 cut(s)	119, 405, 462, 1316, 1620, 1725
Msp20I	TGGCCA	1 cut(s)	1310
MspA1I	CMGCKG	1 cut(s)	1217
MspCI	CTTAAG	1 cut(s)	461
MspI	CCGG	6 cut(s)	131, 145, 399, 1087, 1355, 1711
MspR9I	CCNGG	5 cut(s)	132, 847, 1088, 1356, 1599
MunI	CAATTG	2 cut(s)	1149, 1569
Mva1269I	GAATGC	3 cut(s)	27, 347, 1252
MvaI	CCWGG	2 cut(s)	847, 1599
MvnI	CGCG	1 cut(s)	761
MwoI	GCNNNNNNNGC	2 cut(s)	217, 767
NarI	GGCGCC	1 cut(s)	786
NciI	CCSGG	3 cut(s)	132, 1088, 1356
NdeII	GATC	9 cut(s)	9, 300, 327, 470, 828, 1054, 1368, 1429, 1674
NlaIII	CATG	6 cut(s)	71, 78, 559, 759, 1037, 1514
NlaIV	GGNNCC	3 cut(s)	787, 1353, 1715
NmuCI	GTSAC	1 cut(s)	1646
NspI	RCATGY	2 cut(s)	71, 559
PaeI	GCATGC	1 cut(s)	71
PagI	TCATGA	1 cut(s)	1510
PaqCI	CACCTGC	1 cut(s)	410
PciI	ACATGT	1 cut(s)	555
PcsI	WCGNNNNNNNCGW	1 cut(s)	68
PctI	GAATGC	3 cut(s)	27, 347, 1252
PdmI	GAANNNNTTC	2 cut(s)	1224, 1738
PfeI	GAWTC	5 cut(s)	335, 371, 1016, 1220, 1523
PflFI	GACNNNGTC	1 cut(s)	839
PfoI	TCCNGGA	1 cut(s)	845
PkrI	GCNGC	4 cut(s)	586, 1216, 1252, 1285
PleI	GAGTC	3 cut(s)	189, 794, 981
PluTI	GGCGCC	1 cut(s)	789
PpsI	GAGTC	3 cut(s)	189, 794, 981
PscI	ACATGT	1 cut(s)	555
Psp124BI	GAGCTC	1 cut(s)	1175
Psp6I	CCWGG	2 cut(s)	845, 1597
PspFI	CCCAGC	1 cut(s)	736
PspGI	CCWGG	2 cut(s)	845, 1597
PspN4I	GGNNCC	3 cut(s)	787, 1353, 1715
PspPI	GGNCC	3 cut(s)	128, 922, 1158
PstI	CTGCAG	1 cut(s)	589
PstNI	CAGNNNCTG	1 cut(s)	338
PsuI	RGATCY	1 cut(s)	9
PsyI	GACNNNGTC	1 cut(s)	839
PvuII	CAGCTG	1 cut(s)	1217
RsaI	GTAC	2 cut(s)	668, 1715
RsaNI	GTAC	2 cut(s)	667, 1714
SacI	GAGCTC	1 cut(s)	1175
SalI	GTCGAC	1 cut(s)	204
SaqAI	TTAA	6 cut(s)	119, 405, 462, 1316, 1620, 1725
SatI	GCNGC	4 cut(s)	585, 1215, 1251, 1284
Sau3AI	GATC	9 cut(s)	9, 300, 327, 470, 828, 1054, 1368, 1429, 1674
Sau96I	GGNCC	3 cut(s)	128, 922, 1158
SchI	GAGTC	3 cut(s)	190, 794, 981
ScrFI	CCNGG	5 cut(s)	132, 847, 1088, 1356, 1599
SduI	GDGCHC	4 cut(s)	547, 1175, 1242, 1392
SfaNI	GCATC	5 cut(s)	1077, 1119, 1135, 1349, 1693
SfcI	CTRYAG	1 cut(s)	585
SfoI	GGCGCC	1 cut(s)	787
SmlI	CTYRAG	2 cut(s)	461, 1270
SmoI	CTYRAG	2 cut(s)	461, 1270
SphI	GCATGC	1 cut(s)	71
SsiI	CCGC	1 cut(s)	613
SspDI	GGCGCC	1 cut(s)	785
SspMI	CTAG	4 cut(s)	767, 1229, 1235, 1344
SstI	GAGCTC	1 cut(s)	1175
StyD4I	CCNGG	5 cut(s)	130, 845, 1086, 1354, 1597
StyI	CCWWGG	3 cut(s)	855, 925, 1287
TaaI	ACNGT	3 cut(s)	416, 671, 1556
TaiI	ACGT	2 cut(s)	155, 899
TaqI	TCGA	6 cut(s)	19, 179, 205, 795, 816, 1074
TfiI	GAWTC	5 cut(s)	335, 371, 1016, 1220, 1523
Tru1I	TTAA	6 cut(s)	119, 405, 462, 1316, 1620, 1725
Tru9I	TTAA	6 cut(s)	119, 405, 462, 1316, 1620, 1725
TscAI	CASTG	4 cut(s)	303, 310, 676, 1561
TseFI	GTSAC	1 cut(s)	1646
TseI	GCWGC	4 cut(s)	584, 1214, 1250, 1283
Tsp45I	GTSAC	1 cut(s)	1646
TspDTI	ATGAA	6 cut(s)	623, 943, 1108, 1175, 1499, 1550
TspGWI	ACGGA	2 cut(s)	848, 1127
TspRI	CASTG	4 cut(s)	303, 310, 676, 1561
Tth111I	GACNNNGTC	1 cut(s)	839
Vha464I	CTTAAG	1 cut(s)	461
VneI	GTGCAC	1 cut(s)	1388
XapI	RAATTY	3 cut(s)	49, 230, 687
XbaI	TCTAGA	1 cut(s)	1228
XceI	RCATGY	2 cut(s)	71, 559
XmiI	GTMKAC	2 cut(s)	205, 1180
XmnI	GAANNNNTTC	2 cut(s)	1224, 1738
XspI	CTAG	4 cut(s)	767, 1229, 1235, 1344

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.