MD13G1055000.v1.1

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012314
Physical Location & Seq
Reverse (-)
3853802 .. 3856915
3114 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1055000.v1.1.491

Sequence Viewer

Length: 2913 bp
ATGGCGATTATGGTGTTGAAGACGGCTGAAAACTGCCCAGAAAATGCAATGGAAATTCTGCAGTCGATATCCAAGAGTGTTTGTTTGGCCAAAGATCTTGTGGAGAGATGCCAAATTGGCGTCCAACCATTTTCAGATGCTGAACTGAGGCTCATTATAGCACAACTGGAGGAGGTCATTAAAAACGTAGGAGAATGCTTGAGCATGATACCGCCATTTACATTTGGAGACCAAGAATACGCAGAAGTTGCAGTTCGGTCCCTTTCAAATGAGATGCAGAATGCTCGCCTTAGAACTCAAGCGTCTCAGATCAATGAGCTCAATACAAAGGTGTGGTCCCTGGAGGAGCAACTACAGGAAGAACCGACAGCGACAGAAATGGATTTCTACTCCCTAAATTTTGAGGTTTCCACAGAGAATCCTCACGTTTTGGATACACCTCAACTGGTTGAAATCCTTAAAAGCACTAGCTGGGTAAGCAAAAGAAATGATGCGAACATGAGTGGATCTATAACGACATTGCCACAGGTGGGTGAATACATGGAGCCTCTGTATGAGAGTTTTTTCTGCCCACTGACAAAGAAGATCATGGATGACCCAGTAACCATAAGAAGCGGAGTGACATACGAGAGGAAAGCAATTGTTGAGTGGTTTGAGAAGTTCAATTGCTCGGAAGAAATCTTCTGCCCAACCACTGGCCAGAAGTTGGCAAGTAAAGATTTGAATTCCAATAAAGCTCTAAAATCCACAATAGCGGAGTGGAAGGACAGGAACGAAGCAGAGAGGATTAAGGTTGCCCGAGCAGCATTGTCCTTGGCTAGTTCAGAGCATATGGTGCTTGAAGCAGTAAAAGATGTGCACAGTATCTGCCAAAGGAACCCATACAATAAGATACAAGTTCGTAGTGTCGGAATATTGCCCTTGCTTGTTCAGTGCCTGGAGTACGAAGACAAAGATGTTAAATGTGCAGCGCTGGAACTTTTGCAGCAACTGGCGGAAGATGATAACAACAGCAAGGAAGAGATTGCGCAAACGATAAATATTTCAACAACAATCAAAATGCTTTCTAGTACTCACCAGTCCCTAAGGCATGCATCATTGCTGTTCTTACTTGAGCTTTCAAGATCACAATCATTATGTGAGAAAATCGGATCAGTTACTGGAGCAATCTTTATGCTGGTCACAATTAAATACAGAAGGTATACCGATGTCTTTGCTTCGGAAAAAGCAGAAGAAATCTTGAGTAATTTAGAGCATTTTCCAAATAACATCAAACACATGGCAGAAAATGGACTCATGGAACCCCTCCTAAAGAATCTTAATGAAGGTTGTGAAGAGATAAAGATAGAGATGGCAAGTTACCTCGGGGAAATTGTTGTTGGGCAAGACCAAAAAACATACGTTGCTGAGAAGGCCTCTCCTGCTCTCATCGAAATGGTACATAACGGAAATACGATGGCCAGAAGGGCAGCATTCAATGCTCTGGGGCAACTCTCATTGTACCACCCAAATGGAAAAATACTTCAAGAAGCTGGAATTATACAAGTCATGGTTGAAGAGATGTTCATTCGGAAAATTCAAAACGAACCAATGGACTCAACAAGTGAAGCAGCTGCAATCCTTGCAAACATATTTGAGTCCGGGCTTGAGCTTGAGAACCTCCCAGCTAATTCTCATGGTCACACTTTGACTTCCGACTATGTTGTGTGCAACATCATCTACATGCTCAAGAACGCAACCGCAGATGAACTAAGTAACAACCTTATCAGAATTCTTCTGTTGATTGCCGAGATCCCCAGATCAGCAGCGGCCATTGTCTCGATGGTAAAAGAGACTGAAGCAAGCTACACCCTCATTGAACTCATCAACAACCCACACGAAAAACTTTCCATCACAGCAATCAAGCTACTAACTGTTCTTTCACCCCATATGGGCCACCTACTATCGGAAAGACTCTGCAAAACCAGAGGCCAGCCTGAGAACCTTATCCATAAACCAACTGAAACAACCCTCATCTCCGAGAAGCACGCAGTTTCAGCAAAATTTCTAGCGCAACTTCCCCACGACAACCTGACACTAAACCTAGCTCTTCTATACAATAACACAGTGCCTACAATCCTGCAAGCAATCAGTCAAATCCAAGGAGATGTCACATCAAGCAGGTTCATAAGCGCATACTTAGAAGGCCTAGTGGGAATTCTAGTCAGATTCACAACAACACTATACGAACCCCAAATACTGTTTCTAGCAAGAACCCACAACTTCACTTCAGTATTCACAGAGCTGCTCATGAAAACATCAAGTGATGAAGTTCAGAGGTTATCAGCAATCGGGTTGAAGAATCTTTCCACAGAGTCGATAAAACTATCAAAACTGCCACAAAGCAAGAAAACCAAGTTGTTGTACCTGCCCAAGTACCTACCTTGTGGATCACCGAGGAGGAGGAAAACACCGCTGTGCCCGGTTCACAGAGGGGTATGTTCTTCACAAAACTCATTCTGCATTGTTGATGCGAAGGCGGTTGAGAGGCTGTTAGTGTGTTTGGGGCATGAGAATGCAGAAGTTGTCGAAGCTGCATTGTCGGCAATATGTACATTGTTGGATGACAAAGTTGACGTCGAGAAGGGTGTGATCATGCTGAGTGAGGCTAATGCTGTGCAGCATGTTTTGAATGTGGTGAAAGAGCACAGGAAAGAGGGGCTGTGGCAGAAGTCGCTTTGGGTGATCGAGAAGTTTTTGAATAAAGGCGGGGATAGTAAGTCGGCTTCGGACATATCAAACGACAGGGTTTTGCCTGCCGTACTGGTGAGCGCTTTCCATCATGGAGCTGGAGATACCAGGCAGATCGCTGAGAAAATTTTAAAGCATTTGAATAAGATGCCCGATCCCTGTGCTTCCAACCGTACCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

971

Amino Acids

108.2

Weight (kDa)

5.77

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 186 - 259 1.4e-14 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012314)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68940 AT1G68940 AT1G68940
fragaria_vesca FvH4_4g28940
malus_domestica MD13G1055000.v1.1
prunus_persica Prupe.1G295900_v2.0.a1 Prupe.1G295900_v2.0.a1 Prupe.1G295900_v2.0.a1
pyrus_communis pycom16g04680
rosa_chinensis RchiOBHm_Chr4g0437081
rosa_laevigata RLG00000006470
rosa_multiflora Rmu_ssc0000006.1_g000011
rosa_roxburghii Rroxscaffold_5G00378160
rosa_rugosa Rorug04G0298000
rosa_samantha Rh4AG352700 Rh4BG362000 Rh4CG376300 Rh4DG356200
rosa_wichuraiana Rw4G030880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2619
Acc16I TGCGCA 1 cut(s) 1029
Acc36I ACCTGC 2 cut(s) 2151, 2415
AccB7I CCANNNNNTGG 2 cut(s) 695, 706
AccI GTMKAC 1 cut(s) 1202
AciI CCGC 9 cut(s) 212, 615, 755, 995, 1740, 1808, 2453, 2519, 2748
AclWI GGATC 5 cut(s) 514, 1159, 1786, 2437, 2879
AcoI YGGCCR 4 cut(s) 87, 697, 1458, 1809
AcsI RAATTY 8 cut(s) 54, 397, 724, 1575, 1770, 2042, 2196, 2856
AcuI CTGAAG 2 cut(s) 1857, 2253
AcyI GRCGYC 2 cut(s) 120, 2616
AfaI GTAC 9 cut(s) 944, 1072, 1440, 1502, 2405, 2417, 2593, 2802, 2905
AfeI AGCGCT 2 cut(s) 972, 2812
AfiI CCNNNNNNNGG 5 cut(s) 530, 695, 706, 1931, 1945
AjnI CCWGG 3 cut(s) 339, 936, 2837
AjuI GAANNNNNNNTTGG 4 cut(s) 1362, 1394, 2226, 2258
AleI CACNNNNGTG 1 cut(s) 2455
Alw21I GWGCWC 3 cut(s) 321, 861, 2688
Alw26I GTCTC 4 cut(s) 222, 309, 1822, 1826
Alw44I GTGCAC 1 cut(s) 857
AlwI GGATC 5 cut(s) 514, 1159, 1786, 2437, 2879
AlwNI CAGNNNCTG 5 cut(s) 140, 867, 937, 991, 1160
Ama87I CYCGRG 2 cut(s) 798, 1364
Aor51HI AGCGCT 2 cut(s) 972, 2812
AoxI GGCC 8 cut(s) 87, 697, 1413, 1458, 1809, 1933, 1969, 2185
ApaLI GTGCAC 1 cut(s) 857
ApoI RAATTY 8 cut(s) 54, 397, 724, 1575, 1770, 2042, 2196, 2856
ArsI GACNNNNNNTTYG 4 cut(s) 320, 352, 2601, 2633
AspLEI GCGC 5 cut(s) 973, 1030, 2053, 2174, 2813
AspS9I GGNCC 3 cut(s) 258, 336, 1933
AsuC2I CCSGG 2 cut(s) 1642, 2462
AsuHPI GGTGA 7 cut(s) 545, 1067, 1914, 2424, 2689, 2734, 2818
AvaI CYCGRG 2 cut(s) 798, 1364
AvaII GGWCC 2 cut(s) 258, 336
AxyI CCTNAGG 1 cut(s) 1085
BaeGI GKGCMC 2 cut(s) 861, 2462
BalI TGGCCA 3 cut(s) 89, 699, 1460
BanII GRGCYC 1 cut(s) 321
BarI GAAGNNNNNNTAC 2 cut(s) 2749, 2781
BbsI GAAGAC 2 cut(s) 26, 954
Bbv12I GWGCWC 3 cut(s) 321, 861, 2688
BccI CCATC 5 cut(s) 1345, 1450, 1816, 1898, 2826
BceAI ACGGC 2 cut(s) 39, 2783
BcgI CGANNNNNNTGC 6 cut(s) 266, 300, 1196, 1230, 2873, 2907
BciT130I CCWGG 3 cut(s) 341, 938, 2839
BciVI GTATCC 1 cut(s) 427
BclI TGATCA 1 cut(s) 2631
BcnI CCSGG 2 cut(s) 1642, 2462
BcoDI GTCTC 4 cut(s) 222, 309, 1822, 1826
BfaI CTAG 8 cut(s) 468, 819, 1068, 2048, 2084, 2189, 2201, 2246
BfmI CTRYAG 2 cut(s) 59, 353
BfoI RGCGCY 2 cut(s) 974, 2814
BfuAI ACCTGC 2 cut(s) 2151, 2415
BfuI GTATCC 1 cut(s) 427
BglI GCCNNNNNGGC 2 cut(s) 117, 1466
BglII AGATCT 1 cut(s) 94
BmcAI AGTACT 1 cut(s) 1072
Bme1390I CCNGG 5 cut(s) 341, 938, 1642, 2462, 2839
Bme18I GGWCC 2 cut(s) 258, 336
BmeT110I CYCGRG 2 cut(s) 798, 1364
BmgT120I GGNCC 3 cut(s) 258, 336, 1933
BmiI GGNNCC 5 cut(s) 260, 338, 546, 878, 1302
BmrFI CCNGG 5 cut(s) 341, 938, 1642, 2462, 2839
BmrI ACTGGG 1 cut(s) 593
BmsI GCATC 7 cut(s) 98, 127, 264, 481, 1103, 2500, 2868
BmuI ACTGGG 1 cut(s) 593
BpiI GAAGAC 2 cut(s) 26, 954
BplI GAGNNNNNCTC 2 cut(s) 1400, 1432
BpmI CTGGAG 5 cut(s) 188, 362, 959, 1182, 2850
BpuEI CTTGAG 7 cut(s) 220, 282, 1133, 1261, 1667, 1673, 1712
BpuMI CCSGG 2 cut(s) 1642, 2462
BsaBI GATNNNNATC 1 cut(s) 1129
BsaHI GRCGYC 2 cut(s) 120, 2616
BsaI GGTCTC 1 cut(s) 222
BsaJI CCNNGG 5 cut(s) 339, 813, 1363, 2140, 2435
BsaXI ACNNNNNCTCC 2 cut(s) 536, 566
Bsc4I CCNNNNNNNGG 5 cut(s) 530, 695, 706, 1931, 1945
Bse1I ACTGG 8 cut(s) 171, 450, 599, 700, 996, 1078, 1165, 2808
Bse21I CCTNAGG 1 cut(s) 1085
Bse3DI GCAATG 3 cut(s) 54, 518, 1097
Bse8I GATNNNNATC 1 cut(s) 1129
BseBI CCWGG 3 cut(s) 341, 938, 2839
BseDI CCNNGG 5 cut(s) 339, 813, 1363, 2140, 2435
BseGI GGATG 2 cut(s) 598, 2608
BseJI GATNNNNATC 1 cut(s) 1129
BseLI CCNNNNNNNGG 5 cut(s) 530, 695, 706, 1931, 1945
BseMI GCAATG 3 cut(s) 54, 518, 1097
BseMII CTCAG 6 cut(s) 137, 320, 1398, 1968, 2630, 2841
BseNI ACTGG 8 cut(s) 171, 450, 599, 700, 996, 1078, 1165, 2808
BseRI GAGGAG 4 cut(s) 185, 359, 2452, 2455
BseSI GKGCMC 2 cut(s) 861, 2462
BseYI CCCAGC 2 cut(s) 471, 1663
BsgI GTGCAG 2 cut(s) 987, 2678
BshFI GGCC 8 cut(s) 89, 699, 1415, 1460, 1811, 1935, 1971, 2187
BsiHKAI GWGCWC 3 cut(s) 321, 861, 2688
BsiHKCI CYCGRG 2 cut(s) 798, 1364
BsiSI CCGG 2 cut(s) 1641, 2462
BslFI GGGAC 3 cut(s) 244, 322, 1066
BslI CCNNNNNNNGG 5 cut(s) 530, 695, 706, 1931, 1945
BsmAI GTCTC 4 cut(s) 222, 309, 1822, 1826
BsmBI CGTCTC 1 cut(s) 309
BsmFI GGGAC 3 cut(s) 244, 322, 1066
BsmI GAATGC 4 cut(s) 200, 286, 1472, 2560
BsnI GGCC 8 cut(s) 89, 699, 1415, 1460, 1811, 1935, 1971, 2187
Bso31I GGTCTC 1 cut(s) 222
BsoBI CYCGRG 2 cut(s) 798, 1364
Bsp1286I GDGCHC 4 cut(s) 321, 861, 2462, 2688
Bsp1407I TGTACA 1 cut(s) 2591
BspACI CCGC 9 cut(s) 212, 615, 755, 995, 1740, 1808, 2453, 2519, 2748
BspANI GGCC 8 cut(s) 89, 699, 1415, 1460, 1811, 1935, 1971, 2187
BspCNI CTCAG 6 cut(s) 138, 319, 1399, 1969, 2631, 2842
BspHI TCATGA 1 cut(s) 2289
BspLI GGNNCC 5 cut(s) 260, 338, 546, 878, 1302
BspMAI CTGCAG 1 cut(s) 63
BspMI ACCTGC 2 cut(s) 2151, 2415
BspPI GGATC 5 cut(s) 514, 1159, 1786, 2437, 2879
BspQI GCTCTTC 1 cut(s) 2094
BspTNI GGTCTC 1 cut(s) 222
BsrDI GCAATG 3 cut(s) 54, 518, 1097
BsrGI TGTACA 1 cut(s) 2591
BsrI ACTGG 8 cut(s) 171, 450, 599, 700, 996, 1078, 1165, 2808
BssECI CCNNGG 5 cut(s) 339, 813, 1363, 2140, 2435
BssNAI GTATAC 1 cut(s) 1203
BssNI GRCGYC 2 cut(s) 120, 2616
BssT1I CCWWGG 2 cut(s) 813, 2140
Bst1107I GTATAC 1 cut(s) 1203
Bst2UI CCWGG 3 cut(s) 341, 938, 2839
Bst4CI ACNGT 5 cut(s) 863, 1915, 2107, 2241, 2903
Bst6I CTCTTC 4 cut(s) 1014, 1329, 1551, 2094
BstACI GRCGYC 2 cut(s) 120, 2616
BstAPI GCANNNNNTGC 4 cut(s) 248, 835, 1478, 1622
BstAUI TGTACA 1 cut(s) 2591
BstC8I GCNNGC 7 cut(s) 286, 1092, 1843, 1973, 2028, 2124, 2796
BstF5I GGATG 2 cut(s) 598, 2608
BstH2I RGCGCY 2 cut(s) 974, 2814
BstHHI GCGC 5 cut(s) 973, 1030, 2053, 2174, 2813
BstMAI GTCTC 4 cut(s) 222, 309, 1822, 1826
BstNI CCWGG 3 cut(s) 341, 938, 2839
BstNSI RCATGY 3 cut(s) 1094, 1726, 2666
BstSCI CCNGG 5 cut(s) 339, 936, 1640, 2460, 2837
BstSFI CTRYAG 2 cut(s) 59, 353
BstSLI GKGCMC 2 cut(s) 861, 2462
BstV2I GAAGAC 2 cut(s) 26, 954
BstX2I RGATCY 3 cut(s) 94, 506, 1791
BstXI CCANNNNNNTGG 1 cut(s) 1511
BstYI RGATCY 3 cut(s) 94, 506, 1791
BstZ17I GTATAC 1 cut(s) 1203
Bsu36I CCTNAGG 1 cut(s) 1085
BsuI GTATCC 1 cut(s) 427
BsuRI GGCC 8 cut(s) 89, 699, 1415, 1460, 1811, 1935, 1971, 2187
BtsCI GGATG 2 cut(s) 598, 2608
BtsIMutI CAGTG 4 cut(s) 572, 693, 938, 2112
BveI ACCTGC 2 cut(s) 2151, 2415
Cac8I GCNNGC 7 cut(s) 286, 1092, 1843, 1973, 2028, 2124, 2796
CaiI CAGNNNCTG 5 cut(s) 140, 867, 937, 991, 1160
CciI TCATGA 1 cut(s) 2289
CfoI GCGC 5 cut(s) 973, 1030, 2053, 2174, 2813
Cfr13I GGNCC 3 cut(s) 258, 336, 1933
CseI GACGC 2 cut(s) 109, 291
Csp6I GTAC 9 cut(s) 943, 1071, 1439, 1501, 2404, 2416, 2592, 2801, 2904
CviQI GTAC 9 cut(s) 943, 1071, 1439, 1501, 2404, 2416, 2592, 2801, 2904
DraI TTTAAA 1 cut(s) 2862
EaeI YGGCCR 4 cut(s) 87, 697, 1458, 1809
Eam1104I CTCTTC 4 cut(s) 1014, 1329, 1551, 2094
EarI CTCTTC 4 cut(s) 1014, 1329, 1551, 2094
EciI GGCGGA 1 cut(s) 1010
Ecl136II GAGCTC 1 cut(s) 319
Eco130I CCWWGG 2 cut(s) 813, 2140
Eco147I AGGCCT 2 cut(s) 1415, 2187
Eco24I GRGCYC 1 cut(s) 321
Eco31I GGTCTC 1 cut(s) 222
Eco32I GATATC 1 cut(s) 69
Eco47I GGWCC 2 cut(s) 258, 336
Eco47III AGCGCT 2 cut(s) 972, 2812
Eco53kI GAGCTC 1 cut(s) 319
Eco57I CTGAAG 2 cut(s) 1857, 2253
Eco81I CCTNAGG 1 cut(s) 1085
Eco88I CYCGRG 2 cut(s) 798, 1364
EcoICRI GAGCTC 1 cut(s) 319
EcoRI GAATTC 3 cut(s) 724, 1770, 2196
EcoRII CCWGG 3 cut(s) 339, 936, 2837
EcoRV GATATC 1 cut(s) 69
EcoT14I CCWWGG 2 cut(s) 813, 2140
EcoT22I ATGCAT 1 cut(s) 1096
EcoT38I GRGCYC 1 cut(s) 321
ErhI CCWWGG 2 cut(s) 813, 2140
Esp3I CGTCTC 1 cut(s) 309
FaqI GGGAC 3 cut(s) 244, 322, 1066
FauI CCCGC 1 cut(s) 2741
FauNDI CATATG 2 cut(s) 831, 1929
FbaI TGATCA 1 cut(s) 2631
FblI GTMKAC 1 cut(s) 1202
FokI GGATG 2 cut(s) 605, 2615
FriOI GRGCYC 1 cut(s) 321
FspBI CTAG 8 cut(s) 468, 819, 1068, 2048, 2084, 2189, 2201, 2246
FspI TGCGCA 1 cut(s) 1029
GlaI GCGC 5 cut(s) 972, 1029, 2052, 2173, 2812
GsaI CCCAGC 2 cut(s) 475, 1667
GsuI CTGGAG 5 cut(s) 188, 362, 959, 1182, 2850
HaeII RGCGCY 2 cut(s) 974, 2814
HaeIII GGCC 8 cut(s) 89, 699, 1415, 1460, 1811, 1935, 1971, 2187
HapII CCGG 2 cut(s) 1641, 2462
HgaI GACGC 2 cut(s) 109, 291
HhaI GCGC 5 cut(s) 973, 1030, 2053, 2174, 2813
Hin1I GRCGYC 2 cut(s) 120, 2616
Hin6I GCGC 5 cut(s) 971, 1028, 2051, 2172, 2811
HinP1I GCGC 5 cut(s) 971, 1028, 2051, 2172, 2811
HincII GTYRAC 1 cut(s) 2614
HindII GTYRAC 1 cut(s) 2614
HinfI GANTC 9 cut(s) 418, 1293, 1315, 1595, 1637, 1953, 2208, 2341, 2354
HpaII CCGG 2 cut(s) 1641, 2462
HphI GGTGA 7 cut(s) 545, 1067, 1914, 2424, 2689, 2734, 2818
Hpy166II GTNNAC 4 cut(s) 859, 1203, 2467, 2614
Hpy188III TCNNGA 8 cut(s) 1122, 1240, 1526, 1729, 1819, 2290, 2620, 2728
Hpy8I GTNNAC 4 cut(s) 859, 1203, 2467, 2614
Hpy99I CGWCG 1 cut(s) 2621
HpyAV CCTTC 8 cut(s) 757, 1191, 1319, 1405, 1458, 2177, 2509, 2617
HpyCH4III ACNGT 5 cut(s) 863, 1915, 2107, 2241, 2903
HpyCH4IV ACGT 4 cut(s) 186, 426, 1401, 2616
HpySE526I ACGT 4 cut(s) 186, 426, 1401, 2616
Hsp92I GRCGYC 2 cut(s) 120, 2616
HspAI GCGC 5 cut(s) 971, 1028, 2051, 2172, 2811
Ksp22I TGATCA 1 cut(s) 2631
LguI GCTCTTC 1 cut(s) 2094
LmnI GCTCC 4 cut(s) 346, 544, 1163, 2825
LweI GCATC 7 cut(s) 98, 127, 264, 481, 1103, 2500, 2868
MaeI CTAG 8 cut(s) 468, 819, 1068, 2048, 2084, 2189, 2201, 2246
MaeII ACGT 4 cut(s) 186, 426, 1401, 2616
MaeIII GTNAC 8 cut(s) 601, 619, 1156, 1180, 1358, 1679, 1754, 2149
MfeI CAATTG 2 cut(s) 639, 664
MflI RGATCY 3 cut(s) 94, 506, 1791
MhlI GDGCHC 4 cut(s) 321, 861, 2462, 2688
MlsI TGGCCA 3 cut(s) 89, 699, 1460
MluNI TGGCCA 3 cut(s) 89, 699, 1460
MlyI GAGTC 5 cut(s) 1287, 1589, 1646, 1947, 2363
MmeI TCCRAC 4 cut(s) 148, 889, 1719, 2580
Mox20I TGGCCA 3 cut(s) 89, 699, 1460
Mph1103I ATGCAT 1 cut(s) 1096
MscI TGGCCA 3 cut(s) 89, 699, 1460
MseI TTAA 7 cut(s) 180, 459, 789, 960, 1188, 1320, 2861
MslI CAYNNNNRTG 5 cut(s) 1433, 1509, 1721, 2455, 2553
Msp20I TGGCCA 3 cut(s) 89, 699, 1460
MspA1I CMGCKG 3 cut(s) 1613, 1808, 2455
MspI CCGG 2 cut(s) 1641, 2462
MspR9I CCNGG 5 cut(s) 341, 938, 1642, 2462, 2839
MunI CAATTG 2 cut(s) 639, 664
Mva1269I GAATGC 4 cut(s) 200, 286, 1472, 2560
MvaI CCWGG 3 cut(s) 341, 938, 2839
NciI CCSGG 2 cut(s) 1642, 2462
NdeI CATATG 2 cut(s) 831, 1929
NlaIV GGNNCC 5 cut(s) 260, 338, 546, 878, 1302
NmeAIII GCCGAG 1 cut(s) 1813
NmuCI GTSAC 4 cut(s) 619, 1180, 1679, 2149
NsbI TGCGCA 1 cut(s) 1029
NsiI ATGCAT 1 cut(s) 1096
NspI RCATGY 3 cut(s) 1094, 1726, 2666
OliI CACNNNNGTG 1 cut(s) 2455
PaeI GCATGC 1 cut(s) 1094
PagI TCATGA 1 cut(s) 2289
PceI AGGCCT 2 cut(s) 1415, 2187
PciSI GCTCTTC 1 cut(s) 2094
PctI GAATGC 4 cut(s) 200, 286, 1472, 2560
PfeI GAWTC 4 cut(s) 418, 1315, 2208, 2341
PflMI CCANNNNNTGG 2 cut(s) 695, 706
PleI GAGTC 5 cut(s) 1287, 1589, 1645, 1947, 2362
PpsI GAGTC 5 cut(s) 1287, 1589, 1645, 1947, 2362
Psp124BI GAGCTC 1 cut(s) 321
Psp6I CCWGG 3 cut(s) 339, 936, 2837
PspFI CCCAGC 2 cut(s) 471, 1663
PspGI CCWGG 3 cut(s) 339, 936, 2837
PspN4I GGNNCC 5 cut(s) 260, 338, 546, 878, 1302
PspPI GGNCC 3 cut(s) 258, 336, 1933
PsrI GAACNNNNNNTAC 2 cut(s) 2220, 2252
PstI CTGCAG 1 cut(s) 63
PstNI CAGNNNCTG 5 cut(s) 140, 867, 937, 991, 1160
PsuI RGATCY 3 cut(s) 94, 506, 1791
PvuII CAGCTG 1 cut(s) 1613
RsaI GTAC 9 cut(s) 944, 1072, 1440, 1502, 2405, 2417, 2593, 2802, 2905
RsaNI GTAC 9 cut(s) 943, 1071, 1439, 1501, 2404, 2416, 2592, 2801, 2904
RseI CAYNNNNRTG 5 cut(s) 1433, 1509, 1721, 2455, 2553
SacI GAGCTC 1 cut(s) 321
SapI GCTCTTC 1 cut(s) 2094
SaqAI TTAA 7 cut(s) 180, 459, 789, 960, 1188, 1320, 2861
Sau96I GGNCC 3 cut(s) 258, 336, 1933
ScaI AGTACT 1 cut(s) 1072
SchI GAGTC 5 cut(s) 1287, 1589, 1646, 1947, 2363
ScrFI CCNGG 5 cut(s) 341, 938, 1642, 2462, 2839
SduI GDGCHC 4 cut(s) 321, 861, 2462, 2688
SfaNI GCATC 7 cut(s) 98, 127, 264, 481, 1103, 2500, 2868
SfcI CTRYAG 2 cut(s) 59, 353
SinI GGWCC 2 cut(s) 258, 336
SmiMI CAYNNNNRTG 5 cut(s) 1433, 1509, 1721, 2455, 2553
SmlI CTYRAG 7 cut(s) 199, 297, 1112, 1240, 1646, 1652, 1727
SmoI CTYRAG 7 cut(s) 199, 297, 1112, 1240, 1646, 1652, 1727
SphI GCATGC 1 cut(s) 1094
SseBI AGGCCT 2 cut(s) 1415, 2187
SsiI CCGC 9 cut(s) 212, 615, 755, 995, 1740, 1808, 2453, 2519, 2748
SspI AATATT 2 cut(s) 915, 1042
SspMI CTAG 8 cut(s) 468, 819, 1068, 2048, 2084, 2189, 2201, 2246
SstI GAGCTC 1 cut(s) 321
StuI AGGCCT 2 cut(s) 1415, 2187
StyD4I CCNGG 5 cut(s) 339, 936, 1640, 2460, 2837
StyI CCWWGG 2 cut(s) 813, 2140
TaaI ACNGT 5 cut(s) 863, 1915, 2107, 2241, 2903
TaiI ACGT 4 cut(s) 189, 429, 1404, 2619
TaqI TCGA 7 cut(s) 65, 1431, 1820, 2357, 2568, 2619, 2727
TaqII GACCGA 1 cut(s) 246
TatI WGTACW 2 cut(s) 1070, 2591
TauI GCSGC 1 cut(s) 1811
TfiI GAWTC 4 cut(s) 418, 1315, 2208, 2341
Tru1I TTAA 7 cut(s) 180, 459, 789, 960, 1188, 1320, 2861
Tru9I TTAA 7 cut(s) 180, 459, 789, 960, 1188, 1320, 2861
TscAI CASTG 4 cut(s) 579, 700, 938, 2112
TseFI GTSAC 4 cut(s) 619, 1180, 1679, 2149
Tsp45I GTSAC 4 cut(s) 619, 1180, 1679, 2149
TspDTI ATGAA 6 cut(s) 1338, 1555, 1761, 2155, 2306, 2322
TspGWI ACGGA 1 cut(s) 1461
TspRI CASTG 4 cut(s) 579, 700, 938, 2112
Van91I CCANNNNNTGG 2 cut(s) 695, 706
VneI GTGCAC 1 cut(s) 857
VpaK11BI GGWCC 2 cut(s) 258, 336
XapI RAATTY 8 cut(s) 54, 397, 724, 1575, 1770, 2042, 2196, 2856
XceI RCATGY 3 cut(s) 1094, 1726, 2666
XcmI CCANNNNNNNNNTGG 3 cut(s) 97, 1819, 2825
XmiI GTMKAC 1 cut(s) 1202
XspI CTAG 8 cut(s) 468, 819, 1068, 2048, 2084, 2189, 2201, 2246
ZraI GACGTC 1 cut(s) 2617
ZrmI AGTACT 1 cut(s) 1072
Zsp2I ATGCAT 1 cut(s) 1096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.