MD13G1073600.v1.1

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010826
Physical Location & Seq
Forward (+)
5187467 .. 5189475
2009 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1073600.v1.1.491

Sequence Viewer

Length: 540 bp
ATGGGTCGTAACGATAATTCTGGGAATTTTCTACAAGTTGTGGCTAACAACTTTGATGTTCTTGCTTTGCCTCTAGTCACACTAGTTTATCCCCTATATGCTTCAATTAGGGCAATAGAAACCAAGTCTCGTACTGATGATCAACAATGGCTTACCTATTGGGTTCTGTATTCTCTGATGACAATCTTCGAGCTCACATTCGCCAAAATCCTTGAATGCTTTCCGGTATGGACTTATGCCAAGTTGATATTCACATGTTGGTTGGTGCTGCCACAATTCAACGGCGCTGCATATGTATATCGTCGCTTCATCAGACCCTACTATATGAACCCACAAGTTGCTCATCAAATGTGGTATTTCCCAAGAACGAAGGGCGGTAGCCTCTTCAGCAAGCCGGATGACGTTTTAACCGCTGCCGAAAAGTACATGGAAGAGCACGGGACAGAAGCATTTGAAAGGCTCATAAGCAAGGCTGATAGAGAAAGGAGCAACAGGAAGAGCAACAACTACATGATCTTTGATGACGATTATATGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TB2_DP1_HVA22

Protein Analysis

180

Amino Acids

21.22

Weight (kDa)

6.73

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 31 - 107 4.8e-30 TB2/DP1, HVA22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010826)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69700
fragaria_vesca FvH4_4g31530 FvH4_4g31530
malus_domestica MD13G1073600.v1.1 MD16G1074900.v1.1
prunus_persica Prupe.1G272400_v2.0.a1
pyrus_communis pycom16g06490
rosa_chinensis RchiOBHm_Chr4g0440041
rosa_laevigata RLG00000006213
rosa_multiflora Rmu_sc0001685.1_g000002 Rmu_sc0022520.1_g000002
rosa_roxburghii Rroxscaffold_5G00380980
rosa_rugosa Rorug04G0323400 Rorug04G0323500 Rorug04G0323600
rosa_samantha Rh4AG374400 Rh4BG386700 Rh4CG401200 Rh4DG381000
rosa_wichuraiana Rw4G032030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 375, 411
AcsI RAATTY 1 cut(s) 25
AcuI CTGAAG 1 cut(s) 370
AfaI GTAC 3 cut(s) 133, 425, 536
AflIII ACRYGT 1 cut(s) 254
AgsI TTSAA 4 cut(s) 105, 215, 280, 455
AhlI ACTAGT 1 cut(s) 82
AjuI GAANNNNNNNTTGG 2 cut(s) 233, 265
AluBI AGCT 1 cut(s) 193
AluI AGCT 1 cut(s) 193
Alw21I GWGCWC 2 cut(s) 195, 438
Alw26I GTCTC 1 cut(s) 132
ApeKI GCWGC 3 cut(s) 268, 287, 413
ApoI RAATTY 1 cut(s) 25
Asp700I GAANNNNTTC 1 cut(s) 219
AspLEI GCGC 1 cut(s) 287
BanII GRGCYC 1 cut(s) 195
Bbv12I GWGCWC 2 cut(s) 195, 438
BbvI GCAGC 3 cut(s) 255, 274, 400
BceAI ACGGC 1 cut(s) 298
BclI TGATCA 1 cut(s) 139
BcoDI GTCTC 1 cut(s) 132
BcuI ACTAGT 1 cut(s) 82
BfaI CTAG 2 cut(s) 74, 83
BfoI RGCGCY 1 cut(s) 288
BisI GCNGC 3 cut(s) 269, 288, 414
BlsI GCNGC 3 cut(s) 270, 289, 415
BsaBI GATNNNNATC 1 cut(s) 182
BsaWI WCCGGW 1 cut(s) 223
Bse8I GATNNNNATC 1 cut(s) 182
BseGI GGATG 1 cut(s) 403
BseJI GATNNNNATC 1 cut(s) 182
BseXI GCAGC 3 cut(s) 255, 274, 400
BsiHKAI GWGCWC 2 cut(s) 195, 438
BsiSI CCGG 2 cut(s) 224, 395
BslFI GGGAC 1 cut(s) 454
BsmAI GTCTC 1 cut(s) 132
BsmFI GGGAC 1 cut(s) 454
BsmI GAATGC 1 cut(s) 221
Bsp1286I GDGCHC 2 cut(s) 195, 438
Bsp143I GATC 2 cut(s) 139, 513
BspACI CCGC 2 cut(s) 375, 411
BspQI GCTCTTC 2 cut(s) 426, 491
BssMI GATC 2 cut(s) 139, 513
Bst6I CTCTTC 3 cut(s) 389, 426, 491
BstC8I GCNNGC 1 cut(s) 392
BstF5I GGATG 1 cut(s) 403
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 1 cut(s) 287
BstKTI GATC 2 cut(s) 142, 516
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 2 cut(s) 139, 513
BstMWI GCNNNNNNNGC 1 cut(s) 387
BstNSI RCATGY 1 cut(s) 258
BstV1I GCAGC 3 cut(s) 255, 274, 400
BtsCI GGATG 1 cut(s) 403
Cac8I GCNNGC 1 cut(s) 392
CfoI GCGC 1 cut(s) 287
Csp6I GTAC 3 cut(s) 132, 424, 535
CviAII CATG 3 cut(s) 255, 427, 511
CviJI RGCY 7 cut(s) 44, 151, 193, 381, 394, 460, 473
CviKI_1 RGCY 7 cut(s) 44, 151, 193, 381, 394, 460, 473
CviQI GTAC 3 cut(s) 132, 424, 535
DpnI GATC 2 cut(s) 141, 515
DpnII GATC 2 cut(s) 139, 513
Eam1104I CTCTTC 3 cut(s) 389, 426, 491
EarI CTCTTC 3 cut(s) 389, 426, 491
Ecl136II GAGCTC 1 cut(s) 193
Eco24I GRGCYC 1 cut(s) 195
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 370
EcoICRI GAGCTC 1 cut(s) 193
EcoT38I GRGCYC 1 cut(s) 195
FaeI CATG 3 cut(s) 258, 430, 514
FaqI GGGAC 1 cut(s) 454
FatI CATG 3 cut(s) 254, 426, 510
FauNDI CATATG 1 cut(s) 292
FbaI TGATCA 1 cut(s) 139
Fnu4HI GCNGC 3 cut(s) 269, 288, 414
FokI GGATG 1 cut(s) 410
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 3 cut(s) 269, 288, 414
FspBI CTAG 2 cut(s) 74, 83
GlaI GCGC 1 cut(s) 286
GluI GCNGC 3 cut(s) 269, 288, 414
HaeII RGCGCY 1 cut(s) 288
HapII CCGG 2 cut(s) 224, 395
HhaI GCGC 1 cut(s) 287
Hin1II CATG 3 cut(s) 258, 430, 514
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HpaII CCGG 2 cut(s) 224, 395
Hpy188I TCNGA 2 cut(s) 177, 314
Hpy99I CGWCG 1 cut(s) 306
HpyAV CCTTC 1 cut(s) 364
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 1 cut(s) 290
HpyF10VI GCNNNNNNNGC 1 cut(s) 387
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 3 cut(s) 258, 430, 514
HspAI GCGC 1 cut(s) 285
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 2 cut(s) 139, 513
LguI GCTCTTC 2 cut(s) 426, 491
LmnI GCTCC 1 cut(s) 486
LpnPI CCDG 4 cut(s) 6, 237, 408, 478
Lsp1109I GCAGC 3 cut(s) 255, 274, 400
MaeI CTAG 2 cut(s) 74, 83
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 2 cut(s) 8, 76
MalI GATC 2 cut(s) 141, 515
MboI GATC 2 cut(s) 139, 513
MboII GAAGA 4 cut(s) 178, 376, 443, 508
MhlI GDGCHC 2 cut(s) 195, 438
MluCI AATT 4 cut(s) 16, 25, 105, 275
MnlI CCTC 2 cut(s) 81, 392
MroXI GAANNNNTTC 1 cut(s) 219
MseI TTAA 1 cut(s) 407
MspA1I CMGCKG 1 cut(s) 413
MspI CCGG 2 cut(s) 224, 395
Mva1269I GAATGC 1 cut(s) 221
MwoI GCNNNNNNNGC 1 cut(s) 387
NdeI CATATG 1 cut(s) 292
NdeII GATC 2 cut(s) 139, 513
NlaIII CATG 3 cut(s) 258, 430, 514
NmuCI GTSAC 1 cut(s) 76
NspI RCATGY 1 cut(s) 258
PciI ACATGT 1 cut(s) 254
PciSI GCTCTTC 2 cut(s) 426, 491
PctI GAATGC 1 cut(s) 221
PdmI GAANNNNTTC 1 cut(s) 219
PkrI GCNGC 3 cut(s) 270, 289, 415
PscI ACATGT 1 cut(s) 254
Psp124BI GAGCTC 1 cut(s) 195
RsaI GTAC 3 cut(s) 133, 425, 536
RsaNI GTAC 3 cut(s) 132, 424, 535
SacI GAGCTC 1 cut(s) 195
SapI GCTCTTC 2 cut(s) 426, 491
SaqAI TTAA 1 cut(s) 407
SatI GCNGC 3 cut(s) 269, 288, 414
Sau3AI GATC 2 cut(s) 139, 513
SduI GDGCHC 2 cut(s) 195, 438
SetI ASST 3 cut(s) 158, 195, 405
SpeI ACTAGT 1 cut(s) 82
Sse9I AATT 4 cut(s) 16, 25, 105, 275
SsiI CCGC 2 cut(s) 375, 411
SspMI CTAG 2 cut(s) 74, 83
SstI GAGCTC 1 cut(s) 195
TaiI ACGT 1 cut(s) 405
TaqI TCGA 1 cut(s) 189
TasI AATT 4 cut(s) 16, 25, 105, 275
TatI WGTACW 2 cut(s) 423, 534
Tru1I TTAA 1 cut(s) 407
Tru9I TTAA 1 cut(s) 407
TseFI GTSAC 1 cut(s) 76
TseI GCWGC 3 cut(s) 268, 287, 413
Tsp45I GTSAC 1 cut(s) 76
TspDTI ATGAA 2 cut(s) 298, 341
XapI RAATTY 1 cut(s) 25
XceI RCATGY 1 cut(s) 258
XmnI GAANNNNTTC 1 cut(s) 219
XspI CTAG 2 cut(s) 74, 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.