Rh4AG374400

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0010826
Physical Location & Seq
Forward (+)
68173937 .. 68178847
4911 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG374400.1

Sequence Viewer

Length: 735 bp
ATGGGTCGTTCTACAGGCTCTGGAAATAACTTTCTCCAAGTTGTGGCCAACAACTTTGATGTTCTTGCTTTACCTCTTGTCACTCTAGTTTTCCCCTTATATGCTTCGATTAGGGCAATAGAGACCAAGTCTCGCACTGATGATCAGCAATGGCTTACGTACTGGGTTTTGTATTCTCTCATAACACTCTTCGAGATGACCTTTGCCAAAGTCCTTGAATGCTTCCCGATTTGGGCTTATGCCAAGTTGATCGTGACTTGTTGGTTGGTGCTGCCTCAATTCAATGGCGCAGCATATGTTTATCGCCGCTTCATCCGACCGTTCTACATGAACCCACAAATCATAACTAATGGTCAGCAGATCTGGTATTTCCCAAGAACCAAGGATAAGAAGAACATCTTCAGCAAACCAGATGATGTTTTAACCGCCGCTGAGAAATATATGGAAGAACATGGCACTGAAGCTTTTGAGAGGCTCATAACCAAGACTGATAGGGAAGCAAGAGCCAGGAGGAGCAACAATTACATGATCTTTGATGATGATTATATAACAAACAAAATCAAAGAAAGGGAGAAGAAGAGGCGCGCACGTCAACAACAATCCCCAAAAGCCAACCTTTCACATTCAGGTAAGCTCAATCTTCCTCGCTATAATCTTTGCACGTTTACAATTTCGGTTTTCGCTCGCTGCGAAAAGCAGAAAATCATCGAAACGTTGCGGTGGCGATTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TB2_DP1_HVA22

Protein Analysis

244

Amino Acids

28.83

Weight (kDa)

9.69

Isoelectric Point (pI)

53.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 32 - 108 2.1e-29 TB2/DP1, HVA22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010826)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69700
fragaria_vesca FvH4_4g31530 FvH4_4g31530
malus_domestica MD13G1073600.v1.1 MD16G1074900.v1.1
prunus_persica Prupe.1G272400_v2.0.a1
pyrus_communis pycom16g06490
rosa_chinensis RchiOBHm_Chr4g0440041
rosa_laevigata RLG00000006213
rosa_multiflora Rmu_sc0001685.1_g000002 Rmu_sc0022520.1_g000002
rosa_roxburghii Rroxscaffold_5G00380980
rosa_rugosa Rorug04G0323400 Rorug04G0323500 Rorug04G0323600
rosa_samantha Rh4AG374400 Rh4BG386700 Rh4CG401200 Rh4DG381000
rosa_wichuraiana Rw4G032030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 43
AccII CGCG 1 cut(s) 585
AciI CCGC 4 cut(s) 307, 426, 429, 718
AclI AACGTT 1 cut(s) 713
AcoI YGGCCR 1 cut(s) 45
AcuI CTGAAG 2 cut(s) 385, 480
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 2 cut(s) 43, 232
AgsI TTSAA 2 cut(s) 218, 283
AjiI CACGTC 1 cut(s) 590
AjnI CCWGG 1 cut(s) 506
AluBI AGCT 2 cut(s) 464, 634
AluI AGCT 2 cut(s) 464, 634
Alw26I GTCTC 2 cut(s) 116, 135
AlwNI CAGNNNCTG 1 cut(s) 20
AoxI GGCC 1 cut(s) 45
ApeKI GCWGC 3 cut(s) 271, 290, 687
Asp700I GAANNNNTTC 1 cut(s) 398
AspLEI GCGC 3 cut(s) 290, 585, 587
BalI TGGCCA 1 cut(s) 47
BbvI GCAGC 3 cut(s) 258, 302, 674
BciT130I CCWGG 1 cut(s) 508
BclI TGATCA 1 cut(s) 142
BcoDI GTCTC 2 cut(s) 116, 135
BfaI CTAG 1 cut(s) 86
BfmI CTRYAG 1 cut(s) 12
BglII AGATCT 1 cut(s) 360
BisI GCNGC 5 cut(s) 272, 291, 307, 429, 688
BlsI GCNGC 5 cut(s) 273, 292, 308, 430, 689
Bme1390I CCNGG 1 cut(s) 508
BmgBI CACGTC 1 cut(s) 590
BmrFI CCNGG 1 cut(s) 508
BmrI ACTGGG 1 cut(s) 172
BmuI ACTGGG 1 cut(s) 172
BsaAI YACGTR 1 cut(s) 159
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 381
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 232
Bse1I ACTGG 1 cut(s) 167
Bse3DI GCAATG 1 cut(s) 155
BseBI CCWGG 1 cut(s) 508
BseDI CCNNGG 1 cut(s) 381
BseGI GGATG 1 cut(s) 312
BseLI CCNNNNNNNGG 2 cut(s) 43, 232
BseMI GCAATG 1 cut(s) 155
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 1 cut(s) 167
BsePI GCGCGC 1 cut(s) 583
BseRI GAGGAG 1 cut(s) 526
BseXI GCAGC 3 cut(s) 258, 302, 674
Bsh1236I CGCG 1 cut(s) 585
Bsh1285I CGRYCG 1 cut(s) 320
BshFI GGCC 1 cut(s) 47
BsiEI CGRYCG 1 cut(s) 320
BslI CCNNNNNNNGG 2 cut(s) 43, 232
BsmAI GTCTC 2 cut(s) 116, 135
BsmI GAATGC 1 cut(s) 224
BsnI GGCC 1 cut(s) 47
Bso31I GGTCTC 1 cut(s) 116
Bsp143I GATC 4 cut(s) 142, 249, 360, 528
BspACI CCGC 4 cut(s) 307, 426, 429, 718
BspANI GGCC 1 cut(s) 47
BspCNI CTCAG 1 cut(s) 424
BspFNI CGCG 1 cut(s) 585
BspTNI GGTCTC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 155
BsrI ACTGG 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 381
BssHII GCGCGC 1 cut(s) 583
BssMI GATC 4 cut(s) 142, 249, 360, 528
BssT1I CCWWGG 1 cut(s) 381
Bst2UI CCWGG 1 cut(s) 508
Bst4CI ACNGT 1 cut(s) 321
Bst6I CTCTTC 2 cut(s) 194, 572
BstBAI YACGTR 1 cut(s) 159
BstC8I GCNNGC 2 cut(s) 585, 685
BstDEI CTNAG 1 cut(s) 432
BstF5I GGATG 1 cut(s) 312
BstFNI CGCG 1 cut(s) 585
BstHHI GCGC 3 cut(s) 290, 585, 587
BstKTI GATC 4 cut(s) 145, 252, 363, 531
BstMAI GTCTC 2 cut(s) 116, 135
BstMBI GATC 4 cut(s) 142, 249, 360, 528
BstMCI CGRYCG 1 cut(s) 320
BstNI CCWGG 1 cut(s) 508
BstSCI CCNGG 1 cut(s) 506
BstSFI CTRYAG 1 cut(s) 12
BstSNI TACGTA 1 cut(s) 159
BstUI CGCG 1 cut(s) 585
BstV1I GCAGC 3 cut(s) 258, 302, 674
BstX2I RGATCY 1 cut(s) 360
BstYI RGATCY 1 cut(s) 360
BsuRI GGCC 1 cut(s) 47
BtrI CACGTC 1 cut(s) 590
BtsCI GGATG 1 cut(s) 312
BtsIMutI CAGTG 2 cut(s) 135, 456
Cac8I GCNNGC 2 cut(s) 585, 685
CaiI CAGNNNCTG 1 cut(s) 20
CfoI GCGC 3 cut(s) 290, 585, 587
Csp6I GTAC 1 cut(s) 160
CviAII CATG 3 cut(s) 328, 452, 526
CviJI RGCY 9 cut(s) 18, 47, 154, 236, 464, 475, 506, 611, 634
CviKI_1 RGCY 9 cut(s) 18, 47, 154, 236, 464, 475, 506, 611, 634
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 1 cut(s) 432
DpnI GATC 4 cut(s) 144, 251, 362, 530
DpnII GATC 4 cut(s) 142, 249, 360, 528
EaeI YGGCCR 1 cut(s) 45
Eam1104I CTCTTC 2 cut(s) 194, 572
EarI CTCTTC 2 cut(s) 194, 572
Eco105I TACGTA 1 cut(s) 159
Eco130I CCWWGG 1 cut(s) 381
Eco31I GGTCTC 1 cut(s) 116
Eco57I CTGAAG 2 cut(s) 385, 480
EcoRII CCWGG 1 cut(s) 506
EcoT14I CCWWGG 1 cut(s) 381
ErhI CCWWGG 1 cut(s) 381
FaeI CATG 3 cut(s) 331, 455, 529
FalI AAGNNNNNCTT 4 cut(s) 383, 415, 600, 632
FatI CATG 3 cut(s) 327, 451, 525
FauNDI CATATG 1 cut(s) 295
FbaI TGATCA 1 cut(s) 142
Fnu4HI GCNGC 5 cut(s) 272, 291, 307, 429, 688
FokI GGATG 1 cut(s) 299
Fsp4HI GCNGC 5 cut(s) 272, 291, 307, 429, 688
FspBI CTAG 1 cut(s) 86
GlaI GCGC 3 cut(s) 289, 584, 586
GluI GCNGC 5 cut(s) 272, 291, 307, 429, 688
HaeIII GGCC 1 cut(s) 47
HhaI GCGC 3 cut(s) 290, 585, 587
Hin1II CATG 3 cut(s) 331, 455, 529
Hin6I GCGC 3 cut(s) 288, 583, 585
HinP1I GCGC 3 cut(s) 288, 583, 585
HincII GTYRAC 1 cut(s) 593
HindII GTYRAC 1 cut(s) 593
HindIII AAGCTT 1 cut(s) 462
Hpy166II GTNNAC 2 cut(s) 593, 666
Hpy188I TCNGA 1 cut(s) 317
Hpy188III TCNNGA 4 cut(s) 21, 193, 226, 253
Hpy8I GTNNAC 2 cut(s) 593, 666
HpyCH4III ACNGT 1 cut(s) 321
HpyCH4IV ACGT 4 cut(s) 158, 589, 662, 713
HpyCH4V TGCA 1 cut(s) 660
HpyF3I CTNAG 1 cut(s) 432
HpySE526I ACGT 4 cut(s) 158, 589, 662, 713
Hsp92II CATG 3 cut(s) 331, 455, 529
HspAI GCGC 3 cut(s) 288, 583, 585
Ksp22I TGATCA 1 cut(s) 142
Kzo9I GATC 4 cut(s) 142, 249, 360, 528
LmnI GCTCC 1 cut(s) 513
LpnPI CCDG 7 cut(s) 6, 148, 349, 423, 493, 520, 612
Lsp1109I GCAGC 3 cut(s) 258, 302, 674
MaeI CTAG 1 cut(s) 86
MaeII ACGT 4 cut(s) 158, 589, 662, 713
MaeIII GTNAC 2 cut(s) 79, 253
MalI GATC 4 cut(s) 144, 251, 362, 530
MboI GATC 4 cut(s) 142, 249, 360, 528
MboII GAAGA 7 cut(s) 181, 391, 403, 458, 586, 589, 632
MflI RGATCY 1 cut(s) 360
MlsI TGGCCA 1 cut(s) 47
MluCI AATT 3 cut(s) 278, 520, 669
MluNI TGGCCA 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 340
MnlI CCTC 6 cut(s) 84, 285, 465, 504, 573, 654
Mox20I TGGCCA 1 cut(s) 47
MroXI GAANNNNTTC 1 cut(s) 398
MscI TGGCCA 1 cut(s) 47
MseI TTAA 1 cut(s) 422
Msp20I TGGCCA 1 cut(s) 47
MspA1I CMGCKG 1 cut(s) 431
MspR9I CCNGG 1 cut(s) 508
Mva1269I GAATGC 1 cut(s) 224
MvaI CCWGG 1 cut(s) 508
MvnI CGCG 1 cut(s) 585
NdeI CATATG 1 cut(s) 295
NdeII GATC 4 cut(s) 142, 249, 360, 528
NlaIII CATG 3 cut(s) 331, 455, 529
NmuCI GTSAC 2 cut(s) 79, 253
PauI GCGCGC 1 cut(s) 583
PcsI WCGNNNNNNNCGW 1 cut(s) 687
PctI GAATGC 1 cut(s) 224
PdmI GAANNNNTTC 1 cut(s) 398
PflFI GACNNNGTC 1 cut(s) 127
PflMI CCANNNNNTGG 1 cut(s) 43
PkrI GCNGC 5 cut(s) 273, 292, 308, 430, 689
Ppu21I YACGTR 1 cut(s) 159
Psp1406I AACGTT 1 cut(s) 713
Psp6I CCWGG 1 cut(s) 506
PspGI CCWGG 1 cut(s) 506
PstNI CAGNNNCTG 1 cut(s) 20
PsuI RGATCY 1 cut(s) 360
PsyI GACNNNGTC 1 cut(s) 127
PteI GCGCGC 1 cut(s) 583
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
SaqAI TTAA 1 cut(s) 422
SatI GCNGC 5 cut(s) 272, 291, 307, 429, 688
Sau3AI GATC 4 cut(s) 142, 249, 360, 528
ScrFI CCNGG 1 cut(s) 508
SfcI CTRYAG 1 cut(s) 12
SnaBI TACGTA 1 cut(s) 159
Sse9I AATT 3 cut(s) 278, 520, 669
SsiI CCGC 4 cut(s) 307, 426, 429, 718
SspMI CTAG 1 cut(s) 86
StyD4I CCNGG 1 cut(s) 506
StyI CCWWGG 1 cut(s) 381
TaaI ACNGT 1 cut(s) 321
TaiI ACGT 4 cut(s) 161, 592, 665, 716
TaqI TCGA 3 cut(s) 107, 192, 708
TasI AATT 3 cut(s) 278, 520, 669
TauI GCSGC 2 cut(s) 309, 431
Tru1I TTAA 1 cut(s) 422
Tru9I TTAA 1 cut(s) 422
TscAI CASTG 2 cut(s) 142, 463
TseFI GTSAC 2 cut(s) 79, 253
TseI GCWGC 3 cut(s) 271, 290, 687
Tsp45I GTSAC 2 cut(s) 79, 253
TspDTI ATGAA 2 cut(s) 301, 344
TspRI CASTG 2 cut(s) 142, 463
Tth111I GACNNNGTC 1 cut(s) 127
Van91I CCANNNNNTGG 1 cut(s) 43
XmnI GAANNNNTTC 1 cut(s) 398
XspI CTAG 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.