MD16G1074900.v1.1

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010826
Physical Location & Seq
Forward (+)
5234899 .. 5236313
1415 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1074900.v1.1.491

Sequence Viewer

Length: 543 bp
ATGGGTCGTAACAATAATAATTCTGGGAATTTTCTACAAGTTGTGGCTAACAACTTAGATGTTCTTGCTTTGCCTCTAGTCACATTGGTGTATCCCCTATATGCTTCAATTAGGGCAATAGAAACCAAGTCTCGCACCGATGACCAGCAATGGCTAACCTATTGGGTTCTGTATTCTCTGATGACAATCTTCGAGCTCACATTCGCCAAAGTCCTTGAATGCCTTCCGGTATGGACTTATGCCAAGTTGATATTCACATGTTGGTTGGTGCTGCCACAATTCAATGGAGCTGCATATGTTTATCGACGATTCATCAGACCATACTATATGTACCCACAAACTGCTCATCAGATGTGGTATTTCCCAAGAAAGAAGGGCGCCGGCCTCTTCAGCAAGCCGGATGACGTTTTAACCGCTGCCGAAAAATACATGGAAGAGCATGGGACTGATGCATTTGAAAGGCTCATAAGCAAGGTTGATCGAGAAAGAACCAGCAGGAAGAGCAACAATTACATGATCTTTGATGATGATTATATGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TB2_DP1_HVA22

Protein Analysis

181

Amino Acids

21.32

Weight (kDa)

8.42

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 32 - 108 1.2e-29 TB2/DP1, HVA22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010826)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69700
fragaria_vesca FvH4_4g31530 FvH4_4g31530
malus_domestica MD13G1073600.v1.1 MD16G1074900.v1.1
prunus_persica Prupe.1G272400_v2.0.a1
pyrus_communis pycom16g06490
rosa_chinensis RchiOBHm_Chr4g0440041
rosa_laevigata RLG00000006213
rosa_multiflora Rmu_sc0001685.1_g000002 Rmu_sc0022520.1_g000002
rosa_roxburghii Rroxscaffold_5G00380980
rosa_rugosa Rorug04G0323400 Rorug04G0323500 Rorug04G0323600
rosa_samantha Rh4AG374400 Rh4BG386700 Rh4CG401200 Rh4DG381000
rosa_wichuraiana Rw4G032030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 377
AciI CCGC 1 cut(s) 414
AcsI RAATTY 1 cut(s) 28
AcuI CTGAAG 1 cut(s) 373
AcyI GRCGYC 1 cut(s) 378
AfaI GTAC 2 cut(s) 332, 539
AflIII ACRYGT 1 cut(s) 257
AgsI TTSAA 4 cut(s) 108, 218, 283, 458
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AleI CACNNNNGTG 1 cut(s) 86
AluBI AGCT 2 cut(s) 196, 290
AluI AGCT 2 cut(s) 196, 290
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 1 cut(s) 135
AoxI GGCC 1 cut(s) 382
ApeKI GCWGC 3 cut(s) 271, 290, 416
ApoI RAATTY 1 cut(s) 28
Asp700I GAANNNNTTC 1 cut(s) 222
AspLEI GCGC 1 cut(s) 380
BanI GGYRCC 1 cut(s) 377
BanII GRGCYC 1 cut(s) 198
Bbv12I GWGCWC 1 cut(s) 198
BbvI GCAGC 3 cut(s) 258, 277, 403
BciVI GTATCC 1 cut(s) 102
BcoDI GTCTC 1 cut(s) 135
BfaI CTAG 1 cut(s) 77
BfoI RGCGCY 1 cut(s) 381
BfuI GTATCC 1 cut(s) 102
BisI GCNGC 3 cut(s) 272, 291, 417
BlsI GCNGC 3 cut(s) 273, 292, 418
BmiI GGNNCC 1 cut(s) 379
BmsI GCATC 1 cut(s) 439
BsaBI GATNNNNATC 1 cut(s) 185
BsaHI GRCGYC 1 cut(s) 378
BsaWI WCCGGW 1 cut(s) 226
Bse118I RCCGGY 1 cut(s) 380
Bse3DI GCAATG 1 cut(s) 155
Bse8I GATNNNNATC 1 cut(s) 185
BseGI GGATG 1 cut(s) 406
BseJI GATNNNNATC 1 cut(s) 185
BseMI GCAATG 1 cut(s) 155
BseXI GCAGC 3 cut(s) 258, 277, 403
BshFI GGCC 1 cut(s) 384
BshNI GGYRCC 1 cut(s) 377
BsiHKAI GWGCWC 1 cut(s) 198
BsiSI CCGG 3 cut(s) 227, 381, 398
BslFI GGGAC 1 cut(s) 457
BsmAI GTCTC 1 cut(s) 135
BsmFI GGGAC 1 cut(s) 457
BsmI GAATGC 1 cut(s) 224
BsnI GGCC 1 cut(s) 384
Bsp1286I GDGCHC 1 cut(s) 198
Bsp143I GATC 2 cut(s) 478, 516
BspACI CCGC 1 cut(s) 414
BspANI GGCC 1 cut(s) 384
BspLI GGNNCC 1 cut(s) 379
BspQI GCTCTTC 2 cut(s) 429, 494
BspT107I GGYRCC 1 cut(s) 377
BsrDI GCAATG 1 cut(s) 155
BsrFI RCCGGY 1 cut(s) 380
BssAI RCCGGY 1 cut(s) 380
BssMI GATC 2 cut(s) 478, 516
BssNI GRCGYC 1 cut(s) 378
Bst6I CTCTTC 3 cut(s) 392, 429, 494
BstACI GRCGYC 1 cut(s) 378
BstC8I GCNNGC 2 cut(s) 382, 395
BstDEI CTNAG 1 cut(s) 55
BstF5I GGATG 1 cut(s) 406
BstH2I RGCGCY 1 cut(s) 381
BstHHI GCGC 1 cut(s) 380
BstKTI GATC 2 cut(s) 481, 519
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 2 cut(s) 478, 516
BstMWI GCNNNNNNNGC 2 cut(s) 390, 501
BstNSI RCATGY 1 cut(s) 261
BstV1I GCAGC 3 cut(s) 258, 277, 403
BsuI GTATCC 1 cut(s) 102
BsuRI GGCC 1 cut(s) 384
BtsCI GGATG 1 cut(s) 406
Cac8I GCNNGC 2 cut(s) 382, 395
CfoI GCGC 1 cut(s) 380
Cfr10I RCCGGY 1 cut(s) 380
Csp6I GTAC 2 cut(s) 331, 538
CviAII CATG 4 cut(s) 258, 430, 440, 514
CviJI RGCY 7 cut(s) 47, 154, 196, 290, 384, 397, 463
CviKI_1 RGCY 7 cut(s) 47, 154, 196, 290, 384, 397, 463
CviQI GTAC 2 cut(s) 331, 538
DdeI CTNAG 1 cut(s) 55
DinI GGCGCC 1 cut(s) 379
DpnI GATC 2 cut(s) 480, 518
DpnII GATC 2 cut(s) 478, 516
Eam1104I CTCTTC 3 cut(s) 392, 429, 494
EarI CTCTTC 3 cut(s) 392, 429, 494
Ecl136II GAGCTC 1 cut(s) 196
Eco24I GRGCYC 1 cut(s) 198
Eco53kI GAGCTC 1 cut(s) 196
Eco57I CTGAAG 1 cut(s) 373
EcoICRI GAGCTC 1 cut(s) 196
EcoT22I ATGCAT 1 cut(s) 454
EcoT38I GRGCYC 1 cut(s) 198
EgeI GGCGCC 1 cut(s) 379
EheI GGCGCC 1 cut(s) 379
FaeI CATG 4 cut(s) 261, 433, 443, 517
FaqI GGGAC 1 cut(s) 457
FatI CATG 4 cut(s) 257, 429, 439, 513
FauNDI CATATG 1 cut(s) 295
Fnu4HI GCNGC 3 cut(s) 272, 291, 417
FokI GGATG 1 cut(s) 413
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 3 cut(s) 272, 291, 417
FspBI CTAG 1 cut(s) 77
GlaI GCGC 1 cut(s) 379
GluI GCNGC 3 cut(s) 272, 291, 417
HaeII RGCGCY 1 cut(s) 381
HaeIII GGCC 1 cut(s) 384
HapII CCGG 3 cut(s) 227, 381, 398
HhaI GCGC 1 cut(s) 380
Hin1I GRCGYC 1 cut(s) 378
Hin1II CATG 4 cut(s) 261, 433, 443, 517
Hin6I GCGC 1 cut(s) 378
HinP1I GCGC 1 cut(s) 378
HinfI GANTC 1 cut(s) 309
HpaII CCGG 3 cut(s) 227, 381, 398
Hpy188I TCNGA 3 cut(s) 180, 317, 351
Hpy188III TCNNGA 1 cut(s) 482
Hpy99I CGWCG 1 cut(s) 309
HpyAV CCTTC 2 cut(s) 233, 367
HpyCH4IV ACGT 1 cut(s) 405
HpyCH4V TGCA 2 cut(s) 293, 452
HpyF10VI GCNNNNNNNGC 2 cut(s) 390, 501
HpyF3I CTNAG 1 cut(s) 55
HpySE526I ACGT 1 cut(s) 405
Hsp92I GRCGYC 1 cut(s) 378
Hsp92II CATG 4 cut(s) 261, 433, 443, 517
HspAI GCGC 1 cut(s) 378
KasI GGCGCC 1 cut(s) 377
KroI GCCGGC 1 cut(s) 380
KroNI GCCGGC 1 cut(s) 382
Kzo9I GATC 2 cut(s) 478, 516
LguI GCTCTTC 2 cut(s) 429, 494
LmnI GCTCC 1 cut(s) 287
LpnPI CCDG 7 cut(s) 9, 158, 240, 394, 411, 481, 505
Lsp1109I GCAGC 3 cut(s) 258, 277, 403
LweI GCATC 1 cut(s) 439
MaeI CTAG 1 cut(s) 77
MaeII ACGT 1 cut(s) 405
MaeIII GTNAC 2 cut(s) 8, 79
MalI GATC 2 cut(s) 480, 518
MboI GATC 2 cut(s) 478, 516
MboII GAAGA 4 cut(s) 181, 379, 446, 511
MhlI GDGCHC 1 cut(s) 198
MluCI AATT 5 cut(s) 19, 28, 108, 278, 508
Mly113I GGCGCC 1 cut(s) 378
MnlI CCTC 2 cut(s) 84, 395
Mph1103I ATGCAT 1 cut(s) 454
MroNI GCCGGC 1 cut(s) 380
MroXI GAANNNNTTC 1 cut(s) 222
MseI TTAA 1 cut(s) 410
MslI CAYNNNNRTG 1 cut(s) 86
MspA1I CMGCKG 1 cut(s) 416
MspI CCGG 3 cut(s) 227, 381, 398
Mva1269I GAATGC 1 cut(s) 224
MwoI GCNNNNNNNGC 2 cut(s) 390, 501
NaeI GCCGGC 1 cut(s) 382
NarI GGCGCC 1 cut(s) 378
NdeI CATATG 1 cut(s) 295
NdeII GATC 2 cut(s) 478, 516
NgoMIV GCCGGC 1 cut(s) 380
NlaIII CATG 4 cut(s) 261, 433, 443, 517
NlaIV GGNNCC 1 cut(s) 379
NmuCI GTSAC 1 cut(s) 79
NsiI ATGCAT 1 cut(s) 454
NspI RCATGY 1 cut(s) 261
OliI CACNNNNGTG 1 cut(s) 86
PciI ACATGT 1 cut(s) 257
PciSI GCTCTTC 2 cut(s) 429, 494
PctI GAATGC 1 cut(s) 224
PdiI GCCGGC 1 cut(s) 382
PdmI GAANNNNTTC 1 cut(s) 222
PfeI GAWTC 1 cut(s) 309
PkrI GCNGC 3 cut(s) 273, 292, 418
PluTI GGCGCC 1 cut(s) 381
PscI ACATGT 1 cut(s) 257
Psp124BI GAGCTC 1 cut(s) 198
PspN4I GGNNCC 1 cut(s) 379
RsaI GTAC 2 cut(s) 332, 539
RsaNI GTAC 2 cut(s) 331, 538
RseI CAYNNNNRTG 1 cut(s) 86
SacI GAGCTC 1 cut(s) 198
SapI GCTCTTC 2 cut(s) 429, 494
SaqAI TTAA 1 cut(s) 410
SatI GCNGC 3 cut(s) 272, 291, 417
Sau3AI GATC 2 cut(s) 478, 516
SduI GDGCHC 1 cut(s) 198
SetI ASST 5 cut(s) 161, 198, 292, 408, 477
SfaNI GCATC 1 cut(s) 439
SfoI GGCGCC 1 cut(s) 379
SmiMI CAYNNNNRTG 1 cut(s) 86
Sse9I AATT 5 cut(s) 19, 28, 108, 278, 508
SsiI CCGC 1 cut(s) 414
SspDI GGCGCC 1 cut(s) 377
SspMI CTAG 1 cut(s) 77
SstI GAGCTC 1 cut(s) 198
TaiI ACGT 1 cut(s) 408
TaqI TCGA 3 cut(s) 192, 304, 481
TasI AATT 5 cut(s) 19, 28, 108, 278, 508
TatI WGTACW 1 cut(s) 537
TfiI GAWTC 1 cut(s) 309
Tru1I TTAA 1 cut(s) 410
Tru9I TTAA 1 cut(s) 410
TseFI GTSAC 1 cut(s) 79
TseI GCWGC 3 cut(s) 271, 290, 416
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 1 cut(s) 301
XapI RAATTY 1 cut(s) 28
XceI RCATGY 1 cut(s) 261
XmnI GAANNNNTTC 1 cut(s) 222
XspI CTAG 1 cut(s) 77
Zsp2I ATGCAT 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.