Rorug04G0323400

HVA22-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0010826
Physical Location & Seq
Reverse (-)
49856701 .. 49863377
6677 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0323400.1

Sequence Viewer

Length: 3105 bp
ATGAGTTTGCTTCATGTCTTCGGGTTCGGTTTAAAAGTGGGGCATCTACTTTGGATGCTATGTTGCTGGATTGTATCTGTGATTTCCATGAATTGGTACCTTAATGGTGCAATTATGGACACCAAGAGTGGTAGTTTGCTTGGTGATGGCGCCCGCGCCCTCAAATTGTGGGAGAAGATCCCATTGAACATTACTAAGATCCGCCATCATTACTACCAGTATATTGGGTCCAAGACTTGGTGGAAGAGGGTCTTGGTTTCCTGGGTGGTTGGTTGGACTATTGCTTGCTTTTGGAGCTTCTGCTACATGAGCTCAGTGGCTAGTGAGAAGAGGAAAGAGACTCTTACGAGTATGTGTGATGAGAGAGCAAGGATGTTGCAGGATCAGTTTAATGTGAGCATGAACCATATTCAGGCCATGTCGATTCTGATCTCCACATTCCACCACGGCAAGTATCCATCTGCAATTGACCAGAGAACTTTTGCGAGGTACACTGAAAGAACTGCTTTTGAGAGACCTCTTACAAGTGGCGTGGCATATGCTGTAAGGGTGCTCCACTCGGAGAAAGAACAATTTGAGAAGCAGCAAGGCTGGACTATCAAGAGAATGGATACCCTCGAACACGACCAAGTCCATAAGAATGACTATACCCCAGAATTGCTGGAGCCACCCCCAATTCAGGAAGAATATGCTCCTGTCATATTTGCTCAGGATACTGTTGCACATGTCATTTCTTTCGATATGCTGTCTGGGAAGGAAGATCGAGATAATGTGTTGCGCGCAAGAGAATCAGGAAAGGGGGTCTTGACTGCTCCATTTCGGCTGCTTAAAACTAATAGGCTTGGAGTAATACTGACCTTTGCTGTCTACAAGAGAGATCTACCTTCAAATGCAACTCCAAATGAAAGAATTCAAGCAACCGATGGGTATCTAGGCGGAATCTTTCATATTGAGTCACTTGTGGAGAAGTTACTTCAACAACTTGCAAGCAAGCAAACTATCCTTGTCAATGTGTATGATACTACCAATCATTCGGAGCCAATCAGCATGTATGGTTCAAATGTTTCAGATGATGGATTGCGGCATATTAGTACCTTAAATTTTGGAGATCCCTTAAGGAGGCATGAGATGCACTGCAGATTCAAACATAAGCCACCGTGGCCGTGGTTAGCCATCACAACTTCAATTGGGATCCTTGTGATTGCGTTGCTTGTTGGGCATATTTTTCATGCAACTGTAAATAGGATTGCCAAAGTGGAAGATGATTTCCATAAAATGAGCGATCTCAAGAAACAGGCCGAAGCAGCTGATGTTGCCAAATCTCAGTTCCTTGCAACTGTATCCCATGAGATTAGAACCCCAATGAACGGTGTCCTAGGTATGTTGCACATGCTTATGGATACAGATCTAGATGTAACTCAACAAGATTATGTCAGAACTGCCCAGGGTAGTGGAAAAGCCCTAGTCTCACTTATAAATGAGGTCTTGGACCAGGCAAAAATTGAATCTGGTAAGCTGGAGCTCGAGGCAGTGCGTTTTGATCTGCGAGCAATTTTGGATGATGTTTTGTCACTGTTTTCTGGGAAGTCTCAAGAAAAAGGAGTGGAGCTGGGAGTATACATCTCAGATCAGGTTTCTGAAATGCTTATTGGTGATCCTGGAAGGTTTCGGCAAATTATTACCAATCTCATGGGGAATTCGATCAAATTCACAGAGAAAGGACACATCTTTGTCACTGTCCATTTGTTTGAGGAGCTGATTGACTCGATAGACGTTGAGACTGAATCATCATCGAAAAACACTTTGAGTGGGTTCCCTGTTGCGGATAAACGCCGCAGCTGGGAAGGATTTAGGGGTTTCAGTGAAGAGGGATCTGCAAGCTCGTTCTCATCGTCGTCTGATGTCATCAATCTTATTGTGTCTGTTGAGGACACAGGGGTTGGAATCCCTCTAGAAGCCCAATCTCGTGTTTTCACTCCTTTTATGCAGGTGGGACCATCTATCTCACGGACACATGGTGGCACAGGAATTGGACTGAGCATAAGCAAGTGTTTAGTTGGCCTCATGAGAGGGGAGATTGGTTTTGTGAGCATTCCCAAGGTTGGTTCCACATTTACATTTACCGCTGTTTTCGCCAAAGCCCGCTGTGATTCAAATGAATTTAAGATCCAGCAAATCAACAGCCAAGCAAATGCTGCATCCTCAGACTTTCATGGCATGACAGCATTGGTTGTGGATCACAGACCTGTGAGGGCCAAGATGTCAAGATATCATATCCAAAGGCTTGGAATTCGAGTTGAAGTCGCTTCTGAGTTGCAGCAGGCTTTGTCTTGCATAAGCTCTGGGAATACAACTATAAATATGGTCCTTGTTGAACAGGAGGTTTGGGATAATGATTCACGCAGTTCAGCTCTCTTTATTGATAACTTAAAGAAACTTAAATGTCCGGTTCCTCCTAAGCTGTTCCTTCTTGCCAATTCTATAAGCAGTTGCAGAACAAGCTTTGTGAATTCAGGTGTCTGTACGCCAAGTATTATCATGAAGCCTCTCAGGGCAAGTATGCTTGCTGCCTCACTACAAAGAGCCATGGGAATTGGTAACAAGGGTAATACACGGAATGGTGAGCTCCCAAGTTTGTCTCTCCGCAATCTTCTACTTGGAAGAAAAATTCTTATTGTGGATGATAATAAAGTGAACCTCATAGTAGCTGCTGGTGCTTTGAAAAAGTATGGGGCTACAGTGCAGAGTGCAGACAGCGGTAAAGAGGCAATCTCACTGCTTACACCACCCCATAGTTTTGATGCCTGCTTCATGGATATCCAGATGCCGGAAATGGATGGTTTTGAAGCTACGAGGAGAATACGCGACATAGAACGCAATGTCAGCAATAATATTCAACATGGCAAGGTTTCTGCGGAGGACTATGACAATATTTTATCATGGCATGTACCCATTCTGGCCATGACTGCAGATGTTATTCAGGCCACCCATGAGGAATGCACAAGGTGTGGAATGGATGGATATGTTTCTAAACCATTTGAAGCTGAACAGCTTTATCGAGAAGTTTCACGTTTTCTCCATTCTCCTGCAAAAGGTACTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TB2_DP1_HVA22

Protein Analysis

1034

Amino Acids

115.31

Weight (kDa)

7.21

Isoelectric Point (pI)

44.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 155 - 351 7.4e-38 CHASE domain
HisKA PF00512 439 - 504 4.4e-19 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 637 - 708 2.7e-14 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Receiver_CRE1 PF24896 734 - 864 4.2e-44 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 890 - 1021 1.1e-19 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010826)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69700
fragaria_vesca FvH4_4g31530 FvH4_4g31530
malus_domestica MD13G1073600.v1.1 MD16G1074900.v1.1
prunus_persica Prupe.1G272400_v2.0.a1
pyrus_communis pycom16g06490
rosa_chinensis RchiOBHm_Chr4g0440041
rosa_laevigata RLG00000006213
rosa_multiflora Rmu_sc0001685.1_g000002 Rmu_sc0022520.1_g000002
rosa_roxburghii Rroxscaffold_5G00380980
rosa_rugosa Rorug04G0323400 Rorug04G0323500 Rorug04G0323600
rosa_samantha Rh4AG374400 Rh4BG386700 Rh4CG401200 Rh4DG381000
rosa_wichuraiana Rw4G032030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1475
AarI CACCTGC 1 cut(s) 1978
Acc36I ACCTGC 1 cut(s) 1978
Acc65I GGTACC 1 cut(s) 96
AccB1I GGYRCC 2 cut(s) 96, 149
AccB7I CCANNNNNTGG 2 cut(s) 93, 237
AccI GTMKAC 2 cut(s) 867, 1617
AccII CGCG 3 cut(s) 156, 780, 2865
AcoI YGGCCR 2 cut(s) 1160, 2958
AcsI RAATTY 8 cut(s) 909, 1099, 1696, 1706, 2159, 2289, 2509, 2667
AcyI GRCGYC 1 cut(s) 150
AdeI CACNNNGTG 2 cut(s) 2018, 3006
AfaI GTAC 6 cut(s) 98, 491, 1093, 2524, 2949, 3097
AflII CTTAAG 1 cut(s) 1114
AflIII ACRYGT 1 cut(s) 724
AjnI CCWGG 4 cut(s) 260, 1443, 1491, 1657
AjuI GAANNNNNNNTTGG 6 cut(s) 236, 268, 1310, 1342, 1632, 1664
Alw21I GWGCWC 4 cut(s) 314, 555, 1524, 2628
Alw26I GTCTC 6 cut(s) 332, 508, 1471, 1593, 1772, 2643
Ama87I CYCGRG 1 cut(s) 1523
AoxI GGCC 7 cut(s) 414, 1160, 1296, 2059, 2253, 2958, 2982
ApeKI GCWGC 8 cut(s) 583, 823, 1304, 1836, 2195, 2317, 2567, 2708
ApoI RAATTY 8 cut(s) 909, 1099, 1696, 1706, 2159, 2289, 2509, 2667
Asp700I GAANNNNTTC 1 cut(s) 909
Asp718I GGTACC 1 cut(s) 96
AspA2I CCTAGG 1 cut(s) 1375
AspLEI GCGC 4 cut(s) 152, 158, 780, 782
AspS9I GGNCC 5 cut(s) 228, 1489, 1994, 2253, 2365
AsuHPI GGTGA 3 cut(s) 155, 1664, 2633
AvaI CYCGRG 1 cut(s) 1523
AvaII GGWCC 4 cut(s) 228, 1489, 1994, 2365
AvrII CCTAGG 1 cut(s) 1375
BalI TGGCCA 1 cut(s) 2960
BamHI GGATCC 1 cut(s) 1191
BanI GGYRCC 2 cut(s) 96, 149
BanII GRGCYC 3 cut(s) 314, 1524, 2628
BauI CACGAG 1 cut(s) 1965
BbsI GAAGAC 1 cut(s) 10
Bbv12I GWGCWC 4 cut(s) 314, 555, 1524, 2628
BbvI GCAGC 8 cut(s) 595, 810, 1316, 1848, 2182, 2329, 2554, 2695
BccI CCATC 9 cut(s) 140, 213, 466, 917, 1067, 1181, 2005, 2831, 3011
BceAI ACGGC 2 cut(s) 463, 1147
BciT130I CCWGG 4 cut(s) 262, 1445, 1493, 1659
BciVI GTATCC 5 cut(s) 465, 604, 706, 1351, 1393
BcoDI GTCTC 6 cut(s) 332, 508, 1471, 1593, 1772, 2643
BfaI CTAG 6 cut(s) 321, 932, 1376, 1409, 1463, 1952
BfmI CTRYAG 3 cut(s) 1135, 2736, 2967
BfoI RGCGCY 1 cut(s) 153
BfrI CTTAAG 1 cut(s) 1114
BfuAI ACCTGC 1 cut(s) 1978
BfuI GTATCC 5 cut(s) 465, 604, 706, 1351, 1393
BglI GCCNNNNNGGC 1 cut(s) 1159
BglII AGATCT 2 cut(s) 877, 1405
BlnI CCTAGG 1 cut(s) 1375
Bme1390I CCNGG 4 cut(s) 262, 1445, 1493, 1659
Bme18I GGWCC 4 cut(s) 228, 1489, 1994, 2365
BmeT110I CYCGRG 1 cut(s) 1523
BmgT120I GGNCC 5 cut(s) 228, 1489, 1994, 2253, 2365
BmrFI CCNGG 4 cut(s) 262, 1445, 1493, 1659
BmsI GCATC 6 cut(s) 45, 52, 1119, 2207, 2791, 2814
BpiI GAAGAC 1 cut(s) 10
BplI GAGNNNNNCTC 2 cut(s) 2756, 2788
BpmI CTGGAG 2 cut(s) 683, 1538
Bpu10I CCTNAGC 2 cut(s) 708, 2457
BpuEI CTTGAG 2 cut(s) 1271, 1575
BsaBI GATNNNNATC 3 cut(s) 428, 927, 1404
BsaHI GRCGYC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 508
BsaJI CCNNGG 9 cut(s) 261, 445, 1157, 1163, 1375, 1443, 1444, 2097, 2586
BsaWI WCCGGW 1 cut(s) 2446
BsaXI ACNNNNNCTCC 2 cut(s) 3060, 3090
Bse1I ACTGG 1 cut(s) 217
Bse3DI GCAATG 1 cut(s) 2884
Bse8I GATNNNNATC 3 cut(s) 428, 927, 1404
BseBI CCWGG 4 cut(s) 262, 1445, 1493, 1659
BseDI CCNNGG 9 cut(s) 261, 445, 1157, 1163, 1375, 1443, 1444, 2097, 2586
BseGI GGATG 7 cut(s) 60, 378, 1564, 2198, 2686, 2842, 3022
BseJI GATNNNNATC 3 cut(s) 428, 927, 1404
BseMI GCAATG 1 cut(s) 2884
BseMII CTCAG 8 cut(s) 327, 722, 1337, 1638, 2027, 2217, 2301, 2563
BseNI ACTGG 1 cut(s) 217
BsePI GCGCGC 1 cut(s) 778
BseRI GAGGAG 2 cut(s) 1766, 2869
BseXI GCAGC 8 cut(s) 595, 810, 1316, 1848, 2182, 2329, 2554, 2695
BseYI CCCAGC 2 cut(s) 1609, 1839
BsgI GTGCAG 2 cut(s) 2762, 2769
Bsh1236I CGCG 3 cut(s) 156, 780, 2865
BshFI GGCC 7 cut(s) 416, 1162, 1298, 2061, 2255, 2960, 2984
BshNI GGYRCC 2 cut(s) 96, 149
BsiHKAI GWGCWC 4 cut(s) 314, 555, 1524, 2628
BsiHKCI CYCGRG 1 cut(s) 1523
BsiSI CCGG 2 cut(s) 2447, 2828
BslFI GGGAC 1 cut(s) 2007
BsmAI GTCTC 6 cut(s) 332, 508, 1471, 1593, 1772, 2643
BsmFI GGGAC 1 cut(s) 2007
BsmI GAATGC 2 cut(s) 2091, 3002
BsnI GGCC 7 cut(s) 416, 1162, 1298, 2061, 2255, 2960, 2984
Bso31I GGTCTC 1 cut(s) 508
BsoBI CYCGRG 1 cut(s) 1523
Bsp1286I GDGCHC 4 cut(s) 314, 555, 1524, 2628
Bsp19I CCATGG 1 cut(s) 2586
BspANI GGCC 7 cut(s) 416, 1162, 1298, 2061, 2255, 2960, 2984
BspCNI CTCAG 8 cut(s) 326, 721, 1336, 1637, 2028, 2216, 2302, 2562
BspFNI CGCG 3 cut(s) 156, 780, 2865
BspHI TCATGA 2 cut(s) 2064, 2538
BspMAI CTGCAG 2 cut(s) 1139, 2971
BspMI ACCTGC 1 cut(s) 1978
BspT107I GGYRCC 2 cut(s) 96, 149
BspTI CTTAAG 1 cut(s) 1114
BspTNI GGTCTC 1 cut(s) 508
BsrDI GCAATG 1 cut(s) 2884
BsrI ACTGG 1 cut(s) 217
BssECI CCNNGG 9 cut(s) 261, 445, 1157, 1163, 1375, 1443, 1444, 2097, 2586
BssHII GCGCGC 1 cut(s) 778
BssNAI GTATAC 1 cut(s) 1618
BssNI GRCGYC 1 cut(s) 150
BssSI CACGAG 1 cut(s) 1965
BssT1I CCWWGG 3 cut(s) 1375, 2097, 2586
Bst1107I GTATAC 1 cut(s) 1618
Bst2BI CACGAG 1 cut(s) 1965
Bst2UI CCWGG 4 cut(s) 262, 1445, 1493, 1659
Bst4CI ACNGT 8 cut(s) 718, 1158, 1237, 1339, 1370, 1575, 1738, 2740
Bst6I CTCTTC 3 cut(s) 239, 323, 1860
BstACI GRCGYC 1 cut(s) 150
BstAFI CTTAAG 1 cut(s) 1114
BstAPI GCANNNNNTGC 2 cut(s) 1129, 2195
BstDSI CCRYGG 4 cut(s) 445, 1157, 1163, 2586
BstENI CCTNNNNNAGG 2 cut(s) 1117, 3090
BstF5I GGATG 7 cut(s) 60, 378, 1564, 2198, 2686, 2842, 3022
BstFNI CGCG 3 cut(s) 156, 780, 2865
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 4 cut(s) 152, 158, 780, 782
BstMAI GTCTC 6 cut(s) 332, 508, 1471, 1593, 1772, 2643
BstNI CCWGG 4 cut(s) 262, 1445, 1493, 1659
BstNSI RCATGY 4 cut(s) 728, 1051, 1393, 2948
BstSCI CCNGG 4 cut(s) 260, 1443, 1491, 1657
BstSFI CTRYAG 3 cut(s) 1135, 2736, 2967
BstUI CGCG 3 cut(s) 156, 780, 2865
BstV1I GCAGC 8 cut(s) 595, 810, 1316, 1848, 2182, 2329, 2554, 2695
BstV2I GAAGAC 1 cut(s) 10
BstX2I RGATCY 8 cut(s) 177, 198, 877, 1108, 1191, 1405, 1871, 2166
BstXI CCANNNNNNTGG 4 cut(s) 224, 1451, 1690, 2285
BstYI RGATCY 8 cut(s) 177, 198, 877, 1108, 1191, 1405, 1871, 2166
BstZ17I GTATAC 1 cut(s) 1618
BsuI GTATCC 5 cut(s) 465, 604, 706, 1351, 1393
BsuRI GGCC 7 cut(s) 416, 1162, 1298, 2061, 2255, 2960, 2984
BtgI CCRYGG 4 cut(s) 445, 1157, 1163, 2586
BtsCI GGATG 7 cut(s) 60, 378, 1564, 2198, 2686, 2842, 3022
BtsI GCAGTG 3 cut(s) 1132, 1536, 2774
BtsIMutI CAGTG 9 cut(s) 321, 492, 1132, 1536, 1571, 1734, 1867, 2745, 2774
BveI ACCTGC 1 cut(s) 1978
CciI TCATGA 2 cut(s) 2064, 2538
CfoI GCGC 4 cut(s) 152, 158, 780, 782
Cfr13I GGNCC 5 cut(s) 228, 1489, 1994, 2253, 2365
Csp6I GTAC 6 cut(s) 97, 490, 1092, 2523, 2948, 3096
CspCI CAANNNNNGTGG 2 cut(s) 513, 548
CviQI GTAC 6 cut(s) 97, 490, 1092, 2523, 2948, 3096
DinI GGCGCC 1 cut(s) 151
DraI TTTAAA 1 cut(s) 33
DraIII CACNNNGTG 2 cut(s) 2018, 3006
EaeI YGGCCR 2 cut(s) 1160, 2958
Eam1104I CTCTTC 3 cut(s) 239, 323, 1860
EarI CTCTTC 3 cut(s) 239, 323, 1860
EciI GGCGGA 2 cut(s) 191, 951
Ecl136II GAGCTC 3 cut(s) 312, 1522, 2626
Eco130I CCWWGG 3 cut(s) 1375, 2097, 2586
Eco24I GRGCYC 3 cut(s) 314, 1524, 2628
Eco31I GGTCTC 1 cut(s) 508
Eco32I GATATC 2 cut(s) 2270, 2818
Eco47I GGWCC 4 cut(s) 228, 1489, 1994, 2365
Eco53kI GAGCTC 3 cut(s) 312, 1522, 2626
Eco88I CYCGRG 1 cut(s) 1523
EcoICRI GAGCTC 3 cut(s) 312, 1522, 2626
EcoNI CCTNNNNNAGG 2 cut(s) 1117, 3090
EcoRI GAATTC 4 cut(s) 909, 1696, 2289, 2509
EcoRII CCWGG 4 cut(s) 260, 1443, 1491, 1657
EcoRV GATATC 2 cut(s) 2270, 2818
EcoT14I CCWWGG 3 cut(s) 1375, 2097, 2586
EcoT38I GRGCYC 3 cut(s) 314, 1524, 2628
EgeI GGCGCC 1 cut(s) 151
EheI GGCGCC 1 cut(s) 151
ErhI CCWWGG 3 cut(s) 1375, 2097, 2586
FalI AAGNNNNNCTT 8 cut(s) 236, 268, 327, 359, 490, 522, 788, 820
FaqI GGGAC 1 cut(s) 2007
FauI CCCGC 2 cut(s) 161, 2150
FauNDI CATATG 1 cut(s) 538
FblI GTMKAC 2 cut(s) 867, 1617
FokI GGATG 7 cut(s) 67, 385, 1571, 2185, 2693, 2849, 3029
FriOI GRGCYC 3 cut(s) 314, 1524, 2628
FspBI CTAG 6 cut(s) 321, 932, 1376, 1409, 1463, 1952
GlaI GCGC 4 cut(s) 151, 157, 779, 781
GsaI CCCAGC 2 cut(s) 1613, 1843
GsuI CTGGAG 2 cut(s) 683, 1538
HaeII RGCGCY 1 cut(s) 153
HaeIII GGCC 7 cut(s) 416, 1162, 1298, 2061, 2255, 2960, 2984
HapII CCGG 2 cut(s) 2447, 2828
HhaI GCGC 4 cut(s) 152, 158, 780, 782
Hin1I GRCGYC 1 cut(s) 150
Hin6I GCGC 4 cut(s) 150, 156, 778, 780
HinP1I GCGC 4 cut(s) 150, 156, 778, 780
HindIII AAGCTT 1 cut(s) 2500
HpaII CCGG 2 cut(s) 2447, 2828
HphI GGTGA 3 cut(s) 155, 1664, 2633
Hpy166II GTNNAC 4 cut(s) 492, 868, 1618, 2695
Hpy8I GTNNAC 4 cut(s) 492, 868, 1618, 2695
Hpy99I CGWCG 1 cut(s) 1897
HpyAV CCTTC 5 cut(s) 748, 894, 1656, 1838, 2477
HpyCH4III ACNGT 8 cut(s) 718, 1158, 1237, 1339, 1370, 1575, 1738, 2740
HpyCH4IV ACGT 2 cut(s) 1773, 3070
HpySE526I ACGT 2 cut(s) 1773, 3070
Hsp92I GRCGYC 1 cut(s) 150
HspAI GCGC 4 cut(s) 150, 156, 778, 780
KasI GGCGCC 1 cut(s) 149
KpnI GGTACC 1 cut(s) 100
Lsp1109I GCAGC 8 cut(s) 595, 810, 1316, 1848, 2182, 2329, 2554, 2695
LweI GCATC 6 cut(s) 45, 52, 1119, 2207, 2791, 2814
MaeI CTAG 6 cut(s) 321, 932, 1376, 1409, 1463, 1952
MaeII ACGT 2 cut(s) 1773, 3070
MaeIII GTNAC 6 cut(s) 954, 969, 1414, 1569, 1732, 2597
MfeI CAATTG 2 cut(s) 465, 1185
MflI RGATCY 8 cut(s) 177, 198, 877, 1108, 1191, 1405, 1871, 2166
MhlI GDGCHC 4 cut(s) 314, 555, 1524, 2628
MlsI TGGCCA 1 cut(s) 2960
MluNI TGGCCA 1 cut(s) 2960
Mly113I GGCGCC 1 cut(s) 150
MlyI GAGTC 3 cut(s) 334, 962, 1757
MmeI TCCRAC 2 cut(s) 254, 1921
Mox20I TGGCCA 1 cut(s) 2960
MroXI GAANNNNTTC 1 cut(s) 909
MscI TGGCCA 1 cut(s) 2960
MseI TTAA 9 cut(s) 32, 102, 390, 828, 1097, 1115, 2163, 2429, 2439
MslI CAYNNNNRTG 2 cut(s) 639, 1394
Msp20I TGGCCA 1 cut(s) 2960
MspA1I CMGCKG 5 cut(s) 1307, 1839, 2126, 2145, 2757
MspCI CTTAAG 1 cut(s) 1114
MspI CCGG 2 cut(s) 2447, 2828
MspR9I CCNGG 4 cut(s) 262, 1445, 1493, 1659
MunI CAATTG 2 cut(s) 465, 1185
Mva1269I GAATGC 2 cut(s) 2091, 3002
MvaI CCWGG 4 cut(s) 262, 1445, 1493, 1659
MvnI CGCG 3 cut(s) 156, 780, 2865
NarI GGCGCC 1 cut(s) 150
NcoI CCATGG 1 cut(s) 2586
NdeI CATATG 1 cut(s) 538
NmuCI GTSAC 3 cut(s) 954, 1569, 1732
NspI RCATGY 4 cut(s) 728, 1051, 1393, 2948
PaeR7I CTCGAG 1 cut(s) 1523
PagI TCATGA 2 cut(s) 2064, 2538
PaqCI CACCTGC 1 cut(s) 1978
PasI CCCWGGG 1 cut(s) 1444
PauI GCGCGC 1 cut(s) 778
PciI ACATGT 1 cut(s) 724
PcsI WCGNNNNNNNCGW 1 cut(s) 1889
PctI GAATGC 2 cut(s) 2091, 3002
PdmI GAANNNNTTC 1 cut(s) 909
PfeI GAWTC 9 cut(s) 424, 788, 939, 1140, 1505, 1784, 1944, 2150, 2396
PflFI GACNNNGTC 1 cut(s) 629
PflMI CCANNNNNTGG 2 cut(s) 93, 237
PfoI TCCNGGA 1 cut(s) 1657
PleI GAGTC 3 cut(s) 334, 961, 1757
PluTI GGCGCC 1 cut(s) 153
PpsI GAGTC 3 cut(s) 334, 961, 1757
PscI ACATGT 1 cut(s) 724
PsiI TTATAA 1 cut(s) 1475
Psp124BI GAGCTC 3 cut(s) 314, 1524, 2628
Psp6I CCWGG 4 cut(s) 260, 1443, 1491, 1657
PspFI CCCAGC 2 cut(s) 1609, 1839
PspGI CCWGG 4 cut(s) 260, 1443, 1491, 1657
PspPI GGNCC 5 cut(s) 228, 1489, 1994, 2253, 2365
PspXI VCTCGAGB 1 cut(s) 1523
PstI CTGCAG 2 cut(s) 1139, 2971
PsuI RGATCY 8 cut(s) 177, 198, 877, 1108, 1191, 1405, 1871, 2166
PsyI GACNNNGTC 1 cut(s) 629
PteI GCGCGC 1 cut(s) 778
PvuII CAGCTG 2 cut(s) 1307, 1839
RsaI GTAC 6 cut(s) 98, 491, 1093, 2524, 2949, 3097
RsaNI GTAC 6 cut(s) 97, 490, 1092, 2523, 2948, 3096
RseI CAYNNNNRTG 2 cut(s) 639, 1394
SacI GAGCTC 3 cut(s) 314, 1524, 2628
SaqAI TTAA 9 cut(s) 32, 102, 390, 828, 1097, 1115, 2163, 2429, 2439
Sau96I GGNCC 5 cut(s) 228, 1489, 1994, 2253, 2365
SchI GAGTC 3 cut(s) 334, 962, 1757
ScrFI CCNGG 4 cut(s) 262, 1445, 1493, 1659
SduI GDGCHC 4 cut(s) 314, 555, 1524, 2628
SfaNI GCATC 6 cut(s) 45, 52, 1119, 2207, 2791, 2814
SfcI CTRYAG 3 cut(s) 1135, 2736, 2967
SfoI GGCGCC 1 cut(s) 151
Sfr274I CTCGAG 1 cut(s) 1523
SinI GGWCC 4 cut(s) 228, 1489, 1994, 2365
SlaI CTCGAG 1 cut(s) 1523
SmiMI CAYNNNNRTG 2 cut(s) 639, 1394
SmlI CTYRAG 4 cut(s) 1114, 1286, 1523, 1590
SmoI CTYRAG 4 cut(s) 1114, 1286, 1523, 1590
SspDI GGCGCC 1 cut(s) 149
SspI AATATT 2 cut(s) 2893, 2932
SspMI CTAG 6 cut(s) 321, 932, 1376, 1409, 1463, 1952
SstI GAGCTC 3 cut(s) 314, 1524, 2628
StyD4I CCNGG 4 cut(s) 260, 1443, 1491, 1657
StyI CCWWGG 3 cut(s) 1375, 2097, 2586
TaaI ACNGT 8 cut(s) 718, 1158, 1237, 1339, 1370, 1575, 1738, 2740
TaiI ACGT 2 cut(s) 1776, 3073
TauI GCSGC 2 cut(s) 1084, 1836
TfiI GAWTC 9 cut(s) 424, 788, 939, 1140, 1505, 1784, 1944, 2150, 2396
Tru1I TTAA 9 cut(s) 32, 102, 390, 828, 1097, 1115, 2163, 2429, 2439
Tru9I TTAA 9 cut(s) 32, 102, 390, 828, 1097, 1115, 2163, 2429, 2439
TscAI CASTG 9 cut(s) 321, 499, 1139, 1536, 1578, 1741, 1867, 2745, 2781
TseFI GTSAC 3 cut(s) 954, 1569, 1732
TseI GCWGC 8 cut(s) 583, 823, 1304, 1836, 2195, 2317, 2567, 2708
Tsp45I GTSAC 3 cut(s) 954, 1569, 1732
TspGWI ACGGA 2 cut(s) 2023, 2629
TspRI CASTG 9 cut(s) 321, 499, 1139, 1536, 1578, 1741, 1867, 2745, 2781
Tth111I GACNNNGTC 1 cut(s) 629
Van91I CCANNNNNTGG 2 cut(s) 93, 237
Vha464I CTTAAG 1 cut(s) 1114
VpaK11BI GGWCC 4 cut(s) 228, 1489, 1994, 2365
XagI CCTNNNNNAGG 2 cut(s) 1117, 3090
XapI RAATTY 8 cut(s) 909, 1099, 1696, 1706, 2159, 2289, 2509, 2667
XbaI TCTAGA 2 cut(s) 1408, 1951
XceI RCATGY 4 cut(s) 728, 1051, 1393, 2948
XcmI CCANNNNNNNNNTGG 1 cut(s) 1161
XhoI CTCGAG 1 cut(s) 1523
XmaJI CCTAGG 1 cut(s) 1375
XmiI GTMKAC 2 cut(s) 867, 1617
XmnI GAANNNNTTC 1 cut(s) 909
XspI CTAG 6 cut(s) 321, 932, 1376, 1409, 1463, 1952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.