MD13G1082300.v1.1

Pentatricopeptide repeat-containing protein At1g05670

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005286
Physical Location & Seq
Reverse (-)
5762152 .. 5764975
2824 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1082300.v1.1.491

Sequence Viewer

Length: 2250 bp
ATGAAGAGGCGCGCCATCTTGTCAGTCTATTGTTTTCAGATCTCCTATCATCGGCCCTGCTTAAATCTTGGTTTAAATTCTTCTCTATTCGTTTTTAGGAGATGTTTAAGTCATGGAATTTCCTTGGGATCAACACATACAAGGCCTTTTCCTGACTATTCCCCCAAGAAACCTACCATCAAAGATGCCGAACTTGTACATCTCATCTCCACCACAATTAAGCTTCGCTGCTCTGAGCCTTTTCGCAGTATTCTGAAGCCTTACGAATCTAATTTCAGGTCTGACCATCTTATTTGGGTTCTCATGAATATTAAGAATGACTATAAACTAGTTCTGGATTTCTTTGACTGGGCATGCTTACGAAGGGACCCATCATTAGAAGCCCGTTGTGTCGTTGTACAAATTGCTGCAGCATCAAAGGATCTTGAAACAGCGCATGAGCTCATTCATAATTTTTGGGCAAAACCCAAGTTAGATGTTGGTCTTTCATTTGCTCACTTTTCTGCTCGGTTGATATACACTTACAAGGATTGGGGTTCAGATCCCCATGTGTTTGATGTTTTCTTCCGGGTCCTTGTTGAAACTGAGATGCTCAATGAAGCAAGAAAGCTTTTTGATAAATTGTTGAGCTATGGGTTGGTTATCTCTGTTGATTCCTGTAATTTATTTCTTACTCGGCTATCAAGCACCTTTGATGGGATTGAGATGGCAATAAAATTCTTCAATGAATACCCTGAAGTGGGTATTCATTGGAACACCACATCATACAATATTATCATTCATTGTCTTTGTCGATTAGGGAAAATAAAAGAGGCCCACCATTTACTCCTGCAAATGGAGTTGAGGGGTTGTGTACCTGATGTTGTAAGTTATAGTACTTTAATCAGCAGATATTGTTATGTTGGAGAACTGCAGAAGGTTTTGAAGCTCATAGAGGAAATGAAAATAAAAGGATTGAAGCAAAATTCCTATACGTATAACAGCATAATACTCTTTCTGTGTAAAACGGGTAAACTATCTGAAGCAGAAGGGTTACTGAGGGAGATGATGGTTCTGGGAGTACTTCCTGATAATGTCGTCTACACTACTCTAATTGATGGATTCTGTAAGTTGGGTAATGTTCCTGCTGCATATAGGCTATTTGACGAAATGCGGGATAGGAAAATAATCCCTGATTATATTACATATACTGCTATTATTCATGGATTTTGTCAAATAGGGAAGATGACAGAAGCAGATAAGCTCTTCCATGAAATGGTTAGCAGAGGATTAGAACCAGATGAAGTTACTTATACAGCACTTATTGATGGTTATTGCAAAACAGGCGAGATGAAAGAGGCGTTTTCTCTTCACAACCAGATGGTTAATATGGGACTTACCCCAAATGTTGTCACTTATACGGCATTGGCTGATGGCCTTTGTAAAAGAGGGGAGGTAGATGTAGCAAATGAACTTCTTCATGAGGTGTGCAAGAAGGGCCTTCAACTTAATGTCTGCACTTACAACTCAATTGTTAACAGCCTTTGTAAGTCGGGAAACATAGCGGAGGCAGAAAAATTGGTGGAACAAATGGAGGGTGCAGGGCCCTGTCCTGACGCTATTACTTATACCACTCTGATGGATGCATATTGTAAGATGAGGGATATGGCTAAGGCTCAGAAGGTCCTGCTTGAGATGCTCGATAGAGGGCTTCAACCTACAGTTGTTACATTCAATGTGCTTATGAATGGATTTGGTATGTCAGGAATGGTGGAAGACGCTGAGAGGCTACTTAAGTGGATGTTGGAGAAGGGTATAGTGCCTAATGCGGCAACCTACAATTCTCTCATGAAGCAGTACTGCATTAGAAATAATATGCGTACTACGGCGGAAATATATAGGAGCATGTGTGCTGGTGGGGTGATTCCTGATAGCAACACCTATAACATTTTGATAAAAGGGCATTCTAAGGTAAGGAATATGAAAGAGGCATGGTTTTTGCATAAAGAAATGGCAGGAAAGGGATTTATTCCCACGGCAAGTTGTTACGGTGCACTTATTAAGGGTTTTTTCAAGAGAAGGAAATTTGCAGAGGCAAGGGAACTATTTGAAGAAATGAGGAGACATGGTGTGGTTGCAGATAGAGAAACATACAACATTTTTGTCGATATGAACTATGGAGAAGGGAACATGGACATTACTCTTGATCTTTGCGACGAAATTATAGAGAATTGTCTTGTTGGCAAGCCCAAGAATGAAGAAACTAGCTGA

Protein Analysis

750

Amino Acids

85.34

Weight (kDa)

7.46

Isoelectric Point (pI)

29.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 248 - 280 1.2e-06 PPR repeat
PPR_2 PF13041 252 - 299 4e-14 PPR repeat family
PPR PF01535 255 - 284 4.6e-06 PPR repeat
PPR_3 PF13812 275 - 330 2e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 282 - 315 5e-08 PPR repeat
PPR_2 PF13041 286 - 335 2.1e-13 PPR repeat family
PPR_1 PF12854 317 - 349 1.6e-07 PPR repeat
PPR_2 PF13041 322 - 370 1.9e-13 PPR repeat family
PPR_3 PF13812 345 - 401 2.5e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 353 - 385 4.2e-12 PPR repeat
PPR_2 PF13041 356 - 405 5.6e-17 PPR repeat family
PPR PF01535 359 - 388 6.2e-08 PPR repeat
PPR_1 PF12854 389 - 420 1.4e-08 PPR repeat
PPR_2 PF13041 391 - 440 6.5e-20 PPR repeat family
PPR PF01535 394 - 424 1.7e-08 PPR repeat
PPR_long PF17177 411 - 488 1.2e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 414 - 469 5e-11 Pentatricopeptide repeat domain
TPR_24 PF23276 417 - 520 2.9e-10 Fungal tetratrico peptide repeats
PPR_1 PF12854 422 - 455 5.1e-13 PPR repeat
PPR_2 PF13041 426 - 475 8.7e-20 PPR repeat family
PPR PF01535 429 - 459 1.6e-09 PPR repeat
PPR_3 PF13812 450 - 506 1.1e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 457 - 488 1.6e-09 PPR repeat
PPR_2 PF13041 471 - 510 2.1e-08 PPR repeat family
PPR_long PF17177 490 - 573 4.7e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 492 - 525 1.7e-12 PPR repeat
PPR_2 PF13041 497 - 545 2.6e-14 PPR repeat family
PPR PF01535 500 - 528 4.7e-06 PPR repeat
PPR_3 PF13812 519 - 579 5.3e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 528 - 559 2.9e-08 PPR repeat
PPR_2 PF13041 531 - 578 1.7e-15 PPR repeat family
PPR PF01535 534 - 564 4.7e-07 PPR repeat
PPR_1 PF12854 562 - 594 1.5e-07 PPR repeat
PPR_2 PF13041 566 - 614 3.9e-15 PPR repeat family
PPR_2 PF13041 601 - 650 7.1e-12 PPR repeat family
PPR_2 PF13041 636 - 685 1.7e-10 PPR repeat family
PPR_2 PF13041 671 - 716 6.6e-10 PPR repeat family
PPR PF01535 675 - 704 3.4e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1255
AccI GTMKAC 1 cut(s) 1080
AccII CGCG 1 cut(s) 12
AciI CCGC 4 cut(s) 1153, 1544, 1808, 1868
AclWI GGATC 3 cut(s) 136, 429, 536
AcsI RAATTY 5 cut(s) 76, 117, 716, 964, 2063
AcuI CTGAAG 3 cut(s) 275, 756, 1041
AfaI GTAC 7 cut(s) 198, 399, 855, 877, 1062, 1838, 1861
AfiI CCNNNNNNNGG 6 cut(s) 51, 696, 739, 740, 835, 1255
AflII CTTAAG 1 cut(s) 1772
AhlI ACTAGT 1 cut(s) 328
AluBI AGCT 7 cut(s) 223, 442, 610, 630, 928, 1243, 2247
AluI AGCT 7 cut(s) 223, 442, 610, 630, 928, 1243, 2247
Alw21I GWGCWC 2 cut(s) 444, 2035
Alw26I GTCTC 1 cut(s) 2095
Alw44I GTGCAC 1 cut(s) 2031
AlwI GGATC 3 cut(s) 136, 429, 536
AoxI GGCC 6 cut(s) 53, 143, 813, 1414, 1477, 1583
ApaI GGGCCC 1 cut(s) 1587
ApaLI GTGCAC 1 cut(s) 2031
ApeKI GCWGC 4 cut(s) 228, 407, 410, 1127
ApoI RAATTY 5 cut(s) 76, 117, 716, 964, 2063
AscI GGCGCGCC 1 cut(s) 10
Asp700I GAANNNNTTC 1 cut(s) 1455
AspLEI GCGC 3 cut(s) 12, 14, 436
AspS9I GGNCC 8 cut(s) 54, 367, 571, 814, 1477, 1583, 1584, 1663
AsuC2I CCSGG 1 cut(s) 569
AsuHPI GGTGA 1 cut(s) 1912
AvaII GGWCC 3 cut(s) 367, 571, 1663
BaeGI GKGCMC 2 cut(s) 1587, 2035
BanII GRGCYC 2 cut(s) 444, 1587
BbsI GAAGAC 1 cut(s) 1761
Bbv12I GWGCWC 2 cut(s) 444, 2035
BbvI GCAGC 4 cut(s) 215, 394, 422, 1114
BceAI ACGGC 3 cut(s) 1416, 1881, 2031
BcnI CCSGG 1 cut(s) 569
BcoDI GTCTC 1 cut(s) 2095
BcuI ACTAGT 1 cut(s) 328
BfaI CTAG 2 cut(s) 329, 2244
BfmI CTRYAG 3 cut(s) 408, 911, 1698
BfrI CTTAAG 1 cut(s) 1772
BglII AGATCT 1 cut(s) 39
BisI GCNGC 5 cut(s) 229, 408, 411, 1128, 1809
BlsI GCNGC 5 cut(s) 230, 409, 412, 1129, 1810
BmcAI AGTACT 3 cut(s) 877, 1062, 1838
Bme1390I CCNGG 1 cut(s) 569
Bme18I GGWCC 3 cut(s) 367, 571, 1663
BmgT120I GGNCC 8 cut(s) 54, 367, 571, 814, 1477, 1583, 1584, 1663
BmiI GGNNCC 4 cut(s) 368, 369, 572, 1585
BmrFI CCNGG 1 cut(s) 569
BmrI ACTGGG 1 cut(s) 358
BmsI GCATC 5 cut(s) 175, 422, 579, 1612, 1665
BmuI ACTGGG 1 cut(s) 358
BpiI GAAGAC 1 cut(s) 1761
Bpu10I CCTNAGC 1 cut(s) 1650
BpuEI CTTGAG 1 cut(s) 1691
BpuMI CCSGG 1 cut(s) 569
BsaAI YACGTR 1 cut(s) 975
BsaJI CCNNGG 2 cut(s) 123, 2013
BsaXI ACNNNNNCTCC 4 cut(s) 1873, 1903, 2092, 2122
Bsc4I CCNNNNNNNGG 6 cut(s) 51, 696, 739, 740, 835, 1255
Bse1I ACTGG 1 cut(s) 353
BseDI CCNNGG 2 cut(s) 123, 2013
BseGI GGATG 2 cut(s) 1627, 1785
BseLI CCNNNNNNNGG 6 cut(s) 51, 696, 739, 740, 835, 1255
BseMII CTCAG 5 cut(s) 225, 576, 1028, 1670, 1752
BseNI ACTGG 1 cut(s) 353
BsePI GCGCGC 1 cut(s) 10
BseRI GAGGAG 1 cut(s) 2113
BseSI GKGCMC 2 cut(s) 1587, 2035
BseXI GCAGC 4 cut(s) 215, 394, 422, 1114
BsgI GTGCAG 2 cut(s) 1480, 1599
Bsh1236I CGCG 1 cut(s) 12
BshFI GGCC 6 cut(s) 55, 145, 815, 1416, 1479, 1585
BsiHKAI GWGCWC 2 cut(s) 444, 2035
BsiSI CCGG 1 cut(s) 568
BslFI GGGAC 2 cut(s) 380, 1386
BslI CCNNNNNNNGG 6 cut(s) 51, 696, 739, 740, 835, 1255
BsmAI GTCTC 1 cut(s) 2095
BsmFI GGGAC 2 cut(s) 380, 1386
BsmI GAATGC 1 cut(s) 1942
BsnI GGCC 6 cut(s) 55, 145, 815, 1416, 1479, 1585
Bsp120I GGGCCC 1 cut(s) 1583
Bsp1286I GDGCHC 3 cut(s) 444, 1587, 2035
Bsp1407I TGTACA 2 cut(s) 196, 397
Bsp143I GATC 5 cut(s) 39, 128, 421, 541, 2185
BspACI CCGC 4 cut(s) 1153, 1544, 1808, 1868
BspANI GGCC 6 cut(s) 55, 145, 815, 1416, 1479, 1585
BspCNI CTCAG 5 cut(s) 226, 577, 1029, 1669, 1753
BspFNI CGCG 1 cut(s) 12
BspHI TCATGA 3 cut(s) 303, 1459, 1827
BspLI GGNNCC 4 cut(s) 368, 369, 572, 1585
BspMAI CTGCAG 2 cut(s) 412, 915
BspPI GGATC 3 cut(s) 136, 429, 536
BspQI GCTCTTC 1 cut(s) 1250
BspTI CTTAAG 1 cut(s) 1772
BsrGI TGTACA 2 cut(s) 196, 397
BsrI ACTGG 1 cut(s) 353
BssECI CCNNGG 2 cut(s) 123, 2013
BssHII GCGCGC 1 cut(s) 10
BssMI GATC 5 cut(s) 39, 128, 421, 541, 2185
BssT1I CCWWGG 1 cut(s) 123
Bst4CI ACNGT 2 cut(s) 1702, 2030
Bst6I CTCTTC 2 cut(s) 1250, 1353
BstAFI CTTAAG 1 cut(s) 1772
BstAUI TGTACA 2 cut(s) 196, 397
BstBAI YACGTR 1 cut(s) 975
BstC8I GCNNGC 3 cut(s) 12, 355, 2225
BstDEI CTNAG 7 cut(s) 234, 585, 1037, 1650, 1656, 1761, 1947
BstDSI CCRYGG 1 cut(s) 2013
BstF5I GGATG 2 cut(s) 1627, 1785
BstFNI CGCG 1 cut(s) 12
BstHHI GCGC 3 cut(s) 12, 14, 436
BstKTI GATC 5 cut(s) 42, 131, 424, 544, 2188
BstMAI GTCTC 1 cut(s) 2095
BstMBI GATC 5 cut(s) 39, 128, 421, 541, 2185
BstMWI GCNNNNNNNGC 3 cut(s) 1323, 1476, 1675
BstNSI RCATGY 2 cut(s) 357, 1888
BstSCI CCNGG 1 cut(s) 567
BstSFI CTRYAG 3 cut(s) 408, 911, 1698
BstSLI GKGCMC 2 cut(s) 1587, 2035
BstSNI TACGTA 1 cut(s) 975
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 4 cut(s) 215, 394, 422, 1114
BstV2I GAAGAC 1 cut(s) 1761
BstX2I RGATCY 3 cut(s) 39, 421, 541
BstXI CCANNNNNNTGG 1 cut(s) 1618
BstYI RGATCY 3 cut(s) 39, 421, 541
BsuRI GGCC 6 cut(s) 55, 145, 815, 1416, 1479, 1585
BtgI CCRYGG 1 cut(s) 2013
BtsCI GGATG 2 cut(s) 1627, 1785
Cac8I GCNNGC 3 cut(s) 12, 355, 2225
CciI TCATGA 3 cut(s) 303, 1459, 1827
CfoI GCGC 3 cut(s) 12, 14, 436
Cfr13I GGNCC 8 cut(s) 54, 367, 571, 814, 1477, 1583, 1584, 1663
CseI GACGC 2 cut(s) 1604, 1766
Csp6I GTAC 7 cut(s) 197, 398, 854, 876, 1061, 1837, 1860
CviQI GTAC 7 cut(s) 197, 398, 854, 876, 1061, 1837, 1860
DdeI CTNAG 7 cut(s) 234, 585, 1037, 1650, 1656, 1761, 1947
DpnI GATC 5 cut(s) 41, 130, 423, 543, 2187
DpnII GATC 5 cut(s) 39, 128, 421, 541, 2185
DraI TTTAAA 1 cut(s) 75
Eam1104I CTCTTC 2 cut(s) 1250, 1353
EarI CTCTTC 2 cut(s) 1250, 1353
EciI GGCGGA 1 cut(s) 1883
Ecl136II GAGCTC 1 cut(s) 442
Eco105I TACGTA 1 cut(s) 975
Eco130I CCWWGG 1 cut(s) 123
Eco147I AGGCCT 1 cut(s) 145
Eco24I GRGCYC 2 cut(s) 444, 1587
Eco47I GGWCC 3 cut(s) 367, 571, 1663
Eco53kI GAGCTC 1 cut(s) 442
Eco57I CTGAAG 3 cut(s) 275, 756, 1041
EcoICRI GAGCTC 1 cut(s) 442
EcoO109I RGGNCCY 6 cut(s) 367, 571, 1477, 1583, 1584, 1663
EcoT14I CCWWGG 1 cut(s) 123
EcoT22I ATGCAT 1 cut(s) 1627
EcoT38I GRGCYC 2 cut(s) 444, 1587
ErhI CCWWGG 1 cut(s) 123
FaqI GGGAC 2 cut(s) 380, 1386
FauI CCCGC 1 cut(s) 1146
FblI GTMKAC 1 cut(s) 1080
Fnu4HI GCNGC 5 cut(s) 229, 408, 411, 1128, 1809
FokI GGATG 2 cut(s) 1634, 1792
FriOI GRGCYC 2 cut(s) 444, 1587
Fsp4HI GCNGC 5 cut(s) 229, 408, 411, 1128, 1809
FspBI CTAG 2 cut(s) 329, 2244
GlaI GCGC 3 cut(s) 11, 13, 435
GluI GCNGC 5 cut(s) 229, 408, 411, 1128, 1809
HaeIII GGCC 6 cut(s) 55, 145, 815, 1416, 1479, 1585
HapII CCGG 1 cut(s) 568
HgaI GACGC 2 cut(s) 1604, 1766
HhaI GCGC 3 cut(s) 12, 14, 436
Hin6I GCGC 3 cut(s) 10, 12, 434
HinP1I GCGC 3 cut(s) 10, 12, 434
HincII GTYRAC 1 cut(s) 1516
HindII GTYRAC 1 cut(s) 1516
HindIII AAGCTT 2 cut(s) 221, 608
HinfI GANTC 4 cut(s) 266, 653, 1101, 1903
HpaI GTTAAC 1 cut(s) 1516
HpaII CCGG 1 cut(s) 568
HphI GGTGA 1 cut(s) 1912
Hpy166II GTNNAC 5 cut(s) 854, 1013, 1081, 1516, 2033
Hpy188I TCNGA 8 cut(s) 39, 235, 255, 283, 541, 1021, 1617, 1659
Hpy8I GTNNAC 5 cut(s) 854, 1013, 1081, 1516, 2033
Hpy99I CGWCG 1 cut(s) 2198
HpyAV CCTTC 9 cut(s) 357, 910, 1022, 1468, 1490, 1654, 1783, 2052, 2156
HpyCH4III ACNGT 2 cut(s) 1702, 2030
HpyCH4IV ACGT 1 cut(s) 974
HpyF10VI GCNNNNNNNGC 3 cut(s) 1323, 1476, 1675
HpyF3I CTNAG 7 cut(s) 234, 585, 1037, 1650, 1656, 1761, 1947
HpySE526I ACGT 1 cut(s) 974
HspAI GCGC 3 cut(s) 10, 12, 434
KflI GGGWCCC 1 cut(s) 367
KspAI GTTAAC 1 cut(s) 1516
Kzo9I GATC 5 cut(s) 39, 128, 421, 541, 2185
LguI GCTCTTC 1 cut(s) 1250
LmnI GCTCC 1 cut(s) 1881
Lsp1109I GCAGC 4 cut(s) 215, 394, 422, 1114
LweI GCATC 5 cut(s) 175, 422, 579, 1612, 1665
MaeI CTAG 2 cut(s) 329, 2244
MaeII ACGT 1 cut(s) 974
MaeIII GTNAC 5 cut(s) 1032, 1285, 1390, 1705, 2024
MalI GATC 5 cut(s) 41, 130, 423, 543, 2187
MboI GATC 5 cut(s) 39, 128, 421, 541, 2185
MfeI CAATTG 1 cut(s) 1509
MflI RGATCY 3 cut(s) 39, 421, 541
MhlI GDGCHC 3 cut(s) 444, 1587, 2035
MmeI TCCRAC 2 cut(s) 883, 1764
Mph1103I ATGCAT 1 cut(s) 1627
MroXI GAANNNNTTC 1 cut(s) 1455
MslI CAYNNNNRTG 1 cut(s) 1616
MspCI CTTAAG 1 cut(s) 1772
MspI CCGG 1 cut(s) 568
MspR9I CCNGG 1 cut(s) 569
MunI CAATTG 1 cut(s) 1509
Mva1269I GAATGC 1 cut(s) 1942
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 3 cut(s) 1323, 1476, 1675
NciI CCSGG 1 cut(s) 569
NdeII GATC 5 cut(s) 39, 128, 421, 541, 2185
NlaIV GGNNCC 4 cut(s) 368, 369, 572, 1585
NmeAIII GCCGAG 1 cut(s) 655
NmuCI GTSAC 1 cut(s) 1390
NsiI ATGCAT 1 cut(s) 1627
NspI RCATGY 2 cut(s) 357, 1888
PaeI GCATGC 1 cut(s) 357
PagI TCATGA 3 cut(s) 303, 1459, 1827
PalAI GGCGCGCC 1 cut(s) 10
PauI GCGCGC 1 cut(s) 10
PceI AGGCCT 1 cut(s) 145
PciSI GCTCTTC 1 cut(s) 1250
PctI GAATGC 1 cut(s) 1942
PdmI GAANNNNTTC 1 cut(s) 1455
PfeI GAWTC 4 cut(s) 266, 653, 1101, 1903
PflMI CCANNNNNTGG 1 cut(s) 1255
PkrI GCNGC 5 cut(s) 230, 409, 412, 1129, 1810
Ppu21I YACGTR 1 cut(s) 975
PpuMI RGGWCCY 3 cut(s) 367, 571, 1663
Psp124BI GAGCTC 1 cut(s) 444
Psp5II RGGWCCY 3 cut(s) 367, 571, 1663
PspN4I GGNNCC 4 cut(s) 368, 369, 572, 1585
PspOMI GGGCCC 1 cut(s) 1583
PspPI GGNCC 8 cut(s) 54, 367, 571, 814, 1477, 1583, 1584, 1663
PspPPI RGGWCCY 3 cut(s) 367, 571, 1663
PstI CTGCAG 2 cut(s) 412, 915
PsuI RGATCY 3 cut(s) 39, 421, 541
PteI GCGCGC 1 cut(s) 10
RsaI GTAC 7 cut(s) 198, 399, 855, 877, 1062, 1838, 1861
RsaNI GTAC 7 cut(s) 197, 398, 854, 876, 1061, 1837, 1860
RseI CAYNNNNRTG 1 cut(s) 1616
SacI GAGCTC 1 cut(s) 444
SapI GCTCTTC 1 cut(s) 1250
SatI GCNGC 5 cut(s) 229, 408, 411, 1128, 1809
Sau3AI GATC 5 cut(s) 39, 128, 421, 541, 2185
Sau96I GGNCC 8 cut(s) 54, 367, 571, 814, 1477, 1583, 1584, 1663
ScaI AGTACT 3 cut(s) 877, 1062, 1838
ScrFI CCNGG 1 cut(s) 569
SduI GDGCHC 3 cut(s) 444, 1587, 2035
SfaNI GCATC 5 cut(s) 175, 422, 579, 1612, 1665
SfcI CTRYAG 3 cut(s) 408, 911, 1698
SgsI GGCGCGCC 1 cut(s) 10
SinI GGWCC 3 cut(s) 367, 571, 1663
SmiMI CAYNNNNRTG 1 cut(s) 1616
SmlI CTYRAG 2 cut(s) 1670, 1772
SmoI CTYRAG 2 cut(s) 1670, 1772
SnaBI TACGTA 1 cut(s) 975
SpeI ACTAGT 1 cut(s) 328
SphI GCATGC 1 cut(s) 357
SseBI AGGCCT 1 cut(s) 145
SsiI CCGC 4 cut(s) 1153, 1544, 1808, 1868
SspI AATATT 2 cut(s) 310, 772
SspMI CTAG 2 cut(s) 329, 2244
SstI GAGCTC 1 cut(s) 444
StuI AGGCCT 1 cut(s) 145
StyD4I CCNGG 1 cut(s) 567
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 2 cut(s) 1702, 2030
TaiI ACGT 1 cut(s) 977
TaqI TCGA 3 cut(s) 793, 1680, 2145
TatI WGTACW 5 cut(s) 196, 397, 875, 1060, 1836
TauI GCSGC 1 cut(s) 1811
TfiI GAWTC 4 cut(s) 266, 653, 1101, 1903
TseFI GTSAC 1 cut(s) 1390
TseI GCWGC 4 cut(s) 228, 407, 410, 1127
Tsp45I GTSAC 1 cut(s) 1390
Van91I CCANNNNNTGG 1 cut(s) 1255
Vha464I CTTAAG 1 cut(s) 1772
VneI GTGCAC 1 cut(s) 2031
VpaK11BI GGWCC 3 cut(s) 367, 571, 1663
XapI RAATTY 5 cut(s) 76, 117, 716, 964, 2063
XceI RCATGY 2 cut(s) 357, 1888
XmiI GTMKAC 1 cut(s) 1080
XmnI GAANNNNTTC 1 cut(s) 1455
XspI CTAG 2 cut(s) 329, 2244
ZrmI AGTACT 3 cut(s) 877, 1062, 1838
Zsp2I ATGCAT 1 cut(s) 1627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.