MD16G1081400.v1.1

Pentatricopeptide repeat-containing protein At1g05670

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005286
Physical Location & Seq
Reverse (-)
5709263 .. 5711870
2608 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1081400.v1.1.491

Sequence Viewer

Length: 2247 bp
ATGAAGAGGCGCGCCATCTTGTCAGTCTATAGTTTTCAGATCTCCCATCATCATCTTTGCTTAAATCTTGCTGTAAATTCTTCACTATTCATCTTTAGGAGATGTTTGAGTCATGGAATTTCCTTAGGAGCAACACATACAAGGCCTTTTCCTGACTATTCCCCCAAGAAACCGTCCATCAAAGATGCCGAACTTGTACATCGCATCTCCACCACAATTAAGCTTCGCTGCTCTGAGCCCTTTTGCAGTATTCTGAAGCCTTATGAATCTCATTTCAGTTCTGACCATCTTATTTGGGTCCTCATGAATATTAAGAATGACTATAAACTAGTGTTGGATTTGTTTGACTGGGCATGCTTACGAAGGGACCCATCATTAGAAGCCCATTGTGTTGTTGTACAAATTGCTGCAGCATCAAAGGATCTTAGAACAGCACATGAGCTTATTCATAATTTTTGGGCAAAACCCAAGTTAGATGTTAGTGTTTCATTCACTCGCTTTTCTGATCGGTTGATATACACTTACAAGGATTGGGGTTCAGATCCCCATGTGTTCGATGTTTACTTCCAGGTCCTTGTTGAAACTGGGATGCTCAATGAAGCAAGAAAGCTTTTTGATAAATTGTTGAGCTATGGGTTGGTTATCTCTGTTGATTCCTGTAATTTATTTCTTACTCGGCTATCAAGCACCTTTGATGGGATTGAGATGGCAATAAAGTTTTTCAATGAATATCCTGAAGTGGGTGCTCACTGGAACACCATATCATACAATATTATCATTCATTGTCTTTGTCGATTAGGGAAAATAAAAGAAGCCCACCATTTACTCCTCCAAATGGAGTTGAGGGGTTGTATACCTGATGTTGTAAGTTATAGTACTTTAATCAGCAGATATTGTTATGTTGGAGAACTACAGAAGGTGTTGAAGCTCATCAAGGAAATGAAAATAAAAGGATTGATGCAAAATTCCTATACCTATAACAGCATAATACTCCTTCTGTGTAAGACTGGTAAACTATCTGAAGCAGAAGTGTTACTGAGGGAGATGATGGTTTTAGGAATACTTCCTGATAATGTTGTCTACACTACTCTAATTGATGGATTCTGTAAGTCGGGAAATGTTCCTTCTGCAAGTAGGCTATTTGACGAAATGCAGGGTAGGAAAGTTGTCCCTGATTATATAACATATACTGCCATTATTCATGGATTTTGTCAAATAGGGAAGATGACAGAAGCAGATAAGTTCTTCAATGAAATGGTTAGCAGAGGATTAGAACCAGATGAAGTTACTTATACAGCACTTATTGATGGTTATTGCAAAGCAGGTGAGATGATAGAGGCGTTTTCTCTTCACAACCAGATGGTTAGCATGGGGCTTACCCCAAATGTTGTCACTTATACGGCACTGGCTGATGGGCTTTGTAAAAGAGGGGAGGTAGATGTAGCAAACGAACTTCTTCATGAGGTGTGTAGGAAGGGCCTTCAACTTAATGTCTGCACTTACAACTCAATTGTTAACGGCCTTTGTAAGTCGGGAAACATAGCGGAGGCAGAAAAATTGGTGGAAGAAATGGAGGCTGCTGGGCCCTGTCCTGATACTATTACTTATACCACTTTGATGGATGCATATTGTAAGATGAGAGATATGAGAAAGGCTCAGAAGGTCCTGCATGAGATGCTCGATAGAGGGCTTCAACCTACAGTTGTCACATTCAATGTGCTGATGAATGGATTTTGTATGTCAGGAATGGTGGTAGATGCTGAGAGGCTACTCAAGTGGATGTTGGAGAAGGGTATAGTGCCTAATGCCGCAACCTACAATTCTCTCATGAAGCAGTACTGCATTAGAAATAATATGCGTACTACGACAGAAATTTATAGGAGCATGTGTGCTGGCGGGGTGATTCCTGATAGCAACACCTATAACATTTTGATAAAAGGGCATTGTAAGGCAAGGAATATGAAAGAGGCATGGTTTTTGCATAAAGAAATGGCAGGAAAGGGATTTATTCCCACGTCAAGTTGTTACAGTGCACTTATTAAGGGTTTTTTCAAGAGAAAGAAATTTGCGGAGGCAAGGGAACTATTCGAAGAAATGAGGAGACATGGTGTGGTTGCAGATAGAGAAACATACAACATTTTTGTCGATATGAACTATGAAGAAGGGAACATGGACATTACTCTTGATCTTTGCAATGAAATTATAGAGAATTGTCTTGTTGGCAAGCCCAAGAACGAAGACACGTAG

Protein Analysis

749

Amino Acids

85.14

Weight (kDa)

7.47

Isoelectric Point (pI)

27.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 252 - 280 5.2e-07 PPR repeat
PPR_2 PF13041 252 - 299 2.2e-14 PPR repeat family
PPR PF01535 254 - 284 3.5e-06 PPR repeat
PPR_3 PF13812 275 - 331 1.6e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 282 - 315 4.9e-07 PPR repeat
PPR_2 PF13041 286 - 334 3.1e-12 PPR repeat family
PPR_long PF17177 305 - 407 3.7e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 317 - 349 1.7e-07 PPR repeat
PPR_2 PF13041 322 - 370 1.1e-13 PPR repeat family
PPR PF01535 324 - 354 7.1e-06 PPR repeat
PPR_3 PF13812 345 - 401 3.1e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 353 - 385 2.3e-12 PPR repeat
PPR_2 PF13041 356 - 405 3.6e-16 PPR repeat family
PPR PF01535 359 - 388 1.2e-07 PPR repeat
PPR_1 PF12854 389 - 420 4.6e-09 PPR repeat
PPR_2 PF13041 391 - 440 5.6e-20 PPR repeat family
PPR PF01535 394 - 424 4e-08 PPR repeat
PPR_long PF17177 412 - 486 5e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 416 - 469 9.4e-09 Pentatricopeptide repeat domain
TPR_24 PF23276 419 - 520 1.5e-08 Fungal tetratrico peptide repeats
PPR_1 PF12854 422 - 455 9.9e-13 PPR repeat
PPR_2 PF13041 426 - 475 1.4e-18 PPR repeat family
PPR PF01535 429 - 459 9.6e-10 PPR repeat
PPR_3 PF13812 450 - 506 1.7e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 457 - 488 1.6e-09 PPR repeat
PPR_2 PF13041 471 - 510 5.1e-09 PPR repeat family
PPR_long PF17177 489 - 574 3e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 492 - 525 1.4e-13 PPR repeat
PPR_2 PF13041 497 - 545 1.8e-15 PPR repeat family
PPR PF01535 500 - 528 5e-07 PPR repeat
PPR_3 PF13812 519 - 577 9.1e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 528 - 559 8.9e-10 PPR repeat
PPR_2 PF13041 531 - 579 2.8e-18 PPR repeat family
PPR PF01535 534 - 564 2.8e-08 PPR repeat
PPR_1 PF12854 562 - 594 2.8e-09 PPR repeat
PPR_2 PF13041 566 - 614 1.6e-15 PPR repeat family
PPR_2 PF13041 601 - 650 4.2e-13 PPR repeat family
PPR_long PF17177 615 - 725 5.3e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 633 - 664 5e-08 PPR repeat
PPR_2 PF13041 636 - 685 4.7e-13 PPR repeat family
PPR PF01535 640 - 669 8.7e-07 PPR repeat
PPR_1 PF12854 667 - 700 7.3e-06 PPR repeat
PPR_2 PF13041 671 - 716 1.3e-10 PPR repeat family
PPR PF01535 675 - 704 1.4e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1313
Acc36I ACCTGC 1 cut(s) 1313
AccI GTMKAC 2 cut(s) 853, 1080
AccII CGCG 1 cut(s) 12
AciI CCGC 4 cut(s) 1544, 1809, 1896, 2069
AclWI GGATC 2 cut(s) 429, 536
AcsI RAATTY 5 cut(s) 76, 117, 964, 1872, 2063
AcuI CTGAAG 3 cut(s) 275, 756, 1041
AfaI GTAC 5 cut(s) 198, 399, 877, 1838, 1861
AfiI CCNNNNNNNGG 3 cut(s) 696, 740, 835
AflIII ACRYGT 1 cut(s) 2241
AgsI TTSAA 8 cut(s) 581, 724, 925, 1249, 1484, 1694, 1714, 2053
AhlI ACTAGT 1 cut(s) 328
AjiI CACGTC 1 cut(s) 2016
AjnI CCWGG 1 cut(s) 567
AluBI AGCT 5 cut(s) 223, 442, 610, 630, 928
AluI AGCT 5 cut(s) 223, 442, 610, 630, 928
Alw21I GWGCWC 2 cut(s) 748, 2035
Alw26I GTCTC 1 cut(s) 2095
Alw44I GTGCAC 1 cut(s) 2031
AlwI GGATC 2 cut(s) 429, 536
AoxI GGCC 4 cut(s) 143, 1477, 1519, 1583
ApaI GGGCCC 1 cut(s) 1587
ApaLI GTGCAC 1 cut(s) 2031
ApeKI GCWGC 4 cut(s) 228, 407, 410, 1577
ApoI RAATTY 5 cut(s) 76, 117, 964, 1872, 2063
AscI GGCGCGCC 1 cut(s) 10
Asp700I GAANNNNTTC 1 cut(s) 1455
AspLEI GCGC 2 cut(s) 12, 14
AspS9I GGNCC 7 cut(s) 298, 367, 571, 1477, 1583, 1584, 1663
AsuHPI GGTGA 2 cut(s) 1337, 1912
AsuII TTCGAA 1 cut(s) 2088
AvaII GGWCC 4 cut(s) 298, 367, 571, 1663
AxyI CCTNAGG 1 cut(s) 124
BaeGI GKGCMC 2 cut(s) 1587, 2035
BanII GRGCYC 2 cut(s) 240, 1587
Bbv12I GWGCWC 2 cut(s) 748, 2035
BbvI GCAGC 4 cut(s) 215, 394, 422, 1564
BceAI ACGGC 2 cut(s) 1416, 1534
BciT130I CCWGG 1 cut(s) 569
BcoDI GTCTC 1 cut(s) 2095
BcuI ACTAGT 1 cut(s) 328
BfaI CTAG 1 cut(s) 329
BfmI CTRYAG 4 cut(s) 28, 408, 911, 1698
BfuAI ACCTGC 1 cut(s) 1313
BglII AGATCT 1 cut(s) 39
BisI GCNGC 5 cut(s) 229, 408, 411, 1578, 1809
BlsI GCNGC 5 cut(s) 230, 409, 412, 1579, 1810
BmcAI AGTACT 2 cut(s) 877, 1838
Bme1390I CCNGG 1 cut(s) 569
Bme18I GGWCC 4 cut(s) 298, 367, 571, 1663
BmgBI CACGTC 1 cut(s) 2016
BmgT120I GGNCC 7 cut(s) 298, 367, 571, 1477, 1583, 1584, 1663
BmiI GGNNCC 4 cut(s) 299, 368, 369, 1585
BmrFI CCNGG 1 cut(s) 569
BmrI ACTGGG 2 cut(s) 358, 594
BmsI GCATC 8 cut(s) 175, 213, 422, 579, 948, 1612, 1665, 1747
BmuI ACTGGG 2 cut(s) 358, 594
BplI GAGNNNNNCTC 2 cut(s) 1639, 1671
Bpu14I TTCGAA 1 cut(s) 2088
BpuEI CTTGAG 1 cut(s) 1757
BsaAI YACGTR 1 cut(s) 2244
BsaXI ACNNNNNCTCC 4 cut(s) 1873, 1903, 2092, 2122
Bsc4I CCNNNNNNNGG 3 cut(s) 696, 740, 835
Bse1I ACTGG 5 cut(s) 353, 589, 755, 1012, 1410
Bse21I CCTNAGG 1 cut(s) 124
Bse3DI GCAATG 1 cut(s) 2200
BseBI CCWGG 1 cut(s) 569
BseGI GGATG 3 cut(s) 594, 1627, 1785
BseLI CCNNNNNNNGG 3 cut(s) 696, 740, 835
BseMI GCAATG 1 cut(s) 2200
BseMII CTCAG 4 cut(s) 225, 1028, 1670, 1752
BseNI ACTGG 5 cut(s) 353, 589, 755, 1012, 1410
BsePI GCGCGC 1 cut(s) 10
BseRI GAGGAG 2 cut(s) 818, 2113
BseSI GKGCMC 2 cut(s) 1587, 2035
BseXI GCAGC 4 cut(s) 215, 394, 422, 1564
BseYI CCCAGC 1 cut(s) 1580
BsgI GTGCAG 1 cut(s) 1480
Bsh1236I CGCG 1 cut(s) 12
BshFI GGCC 4 cut(s) 145, 1479, 1521, 1585
BsiHKAI GWGCWC 2 cut(s) 748, 2035
BslFI GGGAC 2 cut(s) 380, 1154
BslI CCNNNNNNNGG 3 cut(s) 696, 740, 835
BsmAI GTCTC 1 cut(s) 2095
BsmFI GGGAC 2 cut(s) 380, 1154
BsnI GGCC 4 cut(s) 145, 1479, 1521, 1585
Bsp119I TTCGAA 1 cut(s) 2088
Bsp120I GGGCCC 1 cut(s) 1583
Bsp1286I GDGCHC 4 cut(s) 240, 748, 1587, 2035
Bsp1407I TGTACA 2 cut(s) 196, 397
Bsp143I GATC 5 cut(s) 39, 421, 505, 541, 2185
BspACI CCGC 4 cut(s) 1544, 1809, 1896, 2069
BspANI GGCC 4 cut(s) 145, 1479, 1521, 1585
BspCNI CTCAG 4 cut(s) 226, 1029, 1669, 1753
BspFNI CGCG 1 cut(s) 12
BspHI TCATGA 3 cut(s) 303, 1459, 1827
BspLI GGNNCC 4 cut(s) 299, 368, 369, 1585
BspMAI CTGCAG 1 cut(s) 412
BspMI ACCTGC 1 cut(s) 1313
BspPI GGATC 2 cut(s) 429, 536
BspT104I TTCGAA 1 cut(s) 2088
BsrDI GCAATG 1 cut(s) 2200
BsrGI TGTACA 2 cut(s) 196, 397
BsrI ACTGG 5 cut(s) 353, 589, 755, 1012, 1410
BssHII GCGCGC 1 cut(s) 10
BssMI GATC 5 cut(s) 39, 421, 505, 541, 2185
BssNAI GTATAC 1 cut(s) 854
Bst1107I GTATAC 1 cut(s) 854
Bst2UI CCWGG 1 cut(s) 569
Bst4CI ACNGT 3 cut(s) 174, 1702, 2030
Bst6I CTCTTC 1 cut(s) 1353
BstAPI GCANNNNNTGC 1 cut(s) 1675
BstAUI TGTACA 2 cut(s) 196, 397
BstBAI YACGTR 1 cut(s) 2244
BstBI TTCGAA 1 cut(s) 2088
BstC8I GCNNGC 4 cut(s) 12, 355, 1894, 2225
BstDEI CTNAG 6 cut(s) 124, 234, 425, 1037, 1656, 1761
BstF5I GGATG 3 cut(s) 594, 1627, 1785
BstFNI CGCG 1 cut(s) 12
BstHHI GCGC 2 cut(s) 12, 14
BstKTI GATC 5 cut(s) 42, 424, 508, 544, 2188
BstMAI GTCTC 1 cut(s) 2095
BstMBI GATC 5 cut(s) 39, 421, 505, 541, 2185
BstMWI GCNNNNNNNGC 1 cut(s) 1675
BstNI CCWGG 1 cut(s) 569
BstNSI RCATGY 2 cut(s) 357, 1888
BstSCI CCNGG 1 cut(s) 567
BstSFI CTRYAG 4 cut(s) 28, 408, 911, 1698
BstSLI GKGCMC 2 cut(s) 1587, 2035
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 4 cut(s) 215, 394, 422, 1564
BstX2I RGATCY 3 cut(s) 39, 421, 541
BstXI CCANNNNNNTGG 1 cut(s) 1618
BstYI RGATCY 3 cut(s) 39, 421, 541
BstZ17I GTATAC 1 cut(s) 854
Bsu36I CCTNAGG 1 cut(s) 124
BsuRI GGCC 4 cut(s) 145, 1479, 1521, 1585
BtgZI GCGATG 1 cut(s) 185
BtrI CACGTC 1 cut(s) 2016
BtsCI GGATG 3 cut(s) 594, 1627, 1785
BtsIMutI CAGTG 3 cut(s) 748, 1403, 2035
BveI ACCTGC 1 cut(s) 1313
Cac8I GCNNGC 4 cut(s) 12, 355, 1894, 2225
CciI TCATGA 3 cut(s) 303, 1459, 1827
CfoI GCGC 2 cut(s) 12, 14
Cfr13I GGNCC 7 cut(s) 298, 367, 571, 1477, 1583, 1584, 1663
Csp6I GTAC 5 cut(s) 197, 398, 876, 1837, 1860
CviQI GTAC 5 cut(s) 197, 398, 876, 1837, 1860
DdeI CTNAG 6 cut(s) 124, 234, 425, 1037, 1656, 1761
DpnI GATC 5 cut(s) 41, 423, 507, 543, 2187
DpnII GATC 5 cut(s) 39, 421, 505, 541, 2185
Eam1104I CTCTTC 1 cut(s) 1353
EarI CTCTTC 1 cut(s) 1353
Eco147I AGGCCT 1 cut(s) 145
Eco24I GRGCYC 2 cut(s) 240, 1587
Eco47I GGWCC 4 cut(s) 298, 367, 571, 1663
Eco57I CTGAAG 3 cut(s) 275, 756, 1041
Eco81I CCTNAGG 1 cut(s) 124
EcoO109I RGGNCCY 6 cut(s) 298, 367, 571, 1477, 1584, 1663
EcoRII CCWGG 1 cut(s) 567
EcoT22I ATGCAT 1 cut(s) 1627
EcoT38I GRGCYC 2 cut(s) 240, 1587
FaqI GGGAC 2 cut(s) 380, 1154
FauI CCCGC 1 cut(s) 1889
FblI GTMKAC 2 cut(s) 853, 1080
Fnu4HI GCNGC 5 cut(s) 229, 408, 411, 1578, 1809
FokI GGATG 3 cut(s) 601, 1634, 1792
FriOI GRGCYC 2 cut(s) 240, 1587
Fsp4HI GCNGC 5 cut(s) 229, 408, 411, 1578, 1809
FspBI CTAG 1 cut(s) 329
GlaI GCGC 2 cut(s) 11, 13
GluI GCNGC 5 cut(s) 229, 408, 411, 1578, 1809
GsaI CCCAGC 1 cut(s) 1584
HaeIII GGCC 4 cut(s) 145, 1479, 1521, 1585
HhaI GCGC 2 cut(s) 12, 14
Hin6I GCGC 2 cut(s) 10, 12
HinP1I GCGC 2 cut(s) 10, 12
HincII GTYRAC 1 cut(s) 1516
HindII GTYRAC 1 cut(s) 1516
HindIII AAGCTT 2 cut(s) 221, 608
HinfI GANTC 5 cut(s) 109, 266, 653, 1101, 1903
HpaI GTTAAC 1 cut(s) 1516
HphI GGTGA 2 cut(s) 1337, 1912
Hpy166II GTNNAC 6 cut(s) 562, 854, 1013, 1081, 1516, 2033
Hpy188I TCNGA 8 cut(s) 39, 235, 255, 283, 505, 541, 1021, 1659
Hpy8I GTNNAC 6 cut(s) 562, 854, 1013, 1081, 1516, 2033
HpyAV CCTTC 9 cut(s) 357, 910, 1004, 1134, 1468, 1490, 1654, 1783, 2156
HpyCH4III ACNGT 3 cut(s) 174, 1702, 2030
HpyCH4IV ACGT 2 cut(s) 2015, 2243
HpyF10VI GCNNNNNNNGC 1 cut(s) 1675
HpyF3I CTNAG 6 cut(s) 124, 234, 425, 1037, 1656, 1761
HpySE526I ACGT 2 cut(s) 2015, 2243
HspAI GCGC 2 cut(s) 10, 12
KflI GGGWCCC 1 cut(s) 367
KspAI GTTAAC 1 cut(s) 1516
Kzo9I GATC 5 cut(s) 39, 421, 505, 541, 2185
LmnI GCTCC 2 cut(s) 128, 1881
Lsp1109I GCAGC 4 cut(s) 215, 394, 422, 1564
LweI GCATC 8 cut(s) 175, 213, 422, 579, 948, 1612, 1665, 1747
MaeI CTAG 1 cut(s) 329
MaeII ACGT 2 cut(s) 2015, 2243
MaeIII GTNAC 5 cut(s) 1032, 1285, 1390, 1705, 2024
MalI GATC 5 cut(s) 41, 423, 507, 543, 2187
MboI GATC 5 cut(s) 39, 421, 505, 541, 2185
MboII GAAGA 9 cut(s) 16, 72, 1234, 1237, 1340, 1448, 1577, 2102, 2171
MfeI CAATTG 1 cut(s) 1509
MflI RGATCY 3 cut(s) 39, 421, 541
MhlI GDGCHC 4 cut(s) 240, 748, 1587, 2035
MlyI GAGTC 1 cut(s) 118
MmeI TCCRAC 3 cut(s) 315, 883, 1764
Mph1103I ATGCAT 1 cut(s) 1627
MroXI GAANNNNTTC 1 cut(s) 1455
MseI TTAA 7 cut(s) 62, 219, 312, 881, 1488, 1515, 2040
MslI CAYNNNNRTG 1 cut(s) 1616
MspR9I CCNGG 1 cut(s) 569
MunI CAATTG 1 cut(s) 1509
MvaI CCWGG 1 cut(s) 569
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 1 cut(s) 1675
NdeII GATC 5 cut(s) 39, 421, 505, 541, 2185
NlaIV GGNNCC 4 cut(s) 299, 368, 369, 1585
NmeAIII GCCGAG 1 cut(s) 655
NmuCI GTSAC 2 cut(s) 1390, 1705
NsiI ATGCAT 1 cut(s) 1627
NspI RCATGY 2 cut(s) 357, 1888
NspV TTCGAA 1 cut(s) 2088
PaeI GCATGC 1 cut(s) 357
PagI TCATGA 3 cut(s) 303, 1459, 1827
PalAI GGCGCGCC 1 cut(s) 10
PaqCI CACCTGC 1 cut(s) 1313
PauI GCGCGC 1 cut(s) 10
PceI AGGCCT 1 cut(s) 145
PdmI GAANNNNTTC 1 cut(s) 1455
PfeI GAWTC 4 cut(s) 266, 653, 1101, 1903
PkrI GCNGC 5 cut(s) 230, 409, 412, 1579, 1810
PleI GAGTC 1 cut(s) 117
PpsI GAGTC 1 cut(s) 117
Ppu21I YACGTR 1 cut(s) 2244
PpuMI RGGWCCY 4 cut(s) 298, 367, 571, 1663
Psp5II RGGWCCY 4 cut(s) 298, 367, 571, 1663
Psp6I CCWGG 1 cut(s) 567
PspFI CCCAGC 1 cut(s) 1580
PspGI CCWGG 1 cut(s) 567
PspN4I GGNNCC 4 cut(s) 299, 368, 369, 1585
PspOMI GGGCCC 1 cut(s) 1583
PspPI GGNCC 7 cut(s) 298, 367, 571, 1477, 1583, 1584, 1663
PspPPI RGGWCCY 4 cut(s) 298, 367, 571, 1663
PstI CTGCAG 1 cut(s) 412
PsuI RGATCY 3 cut(s) 39, 421, 541
PteI GCGCGC 1 cut(s) 10
RsaI GTAC 5 cut(s) 198, 399, 877, 1838, 1861
RsaNI GTAC 5 cut(s) 197, 398, 876, 1837, 1860
RseI CAYNNNNRTG 1 cut(s) 1616
SaqAI TTAA 7 cut(s) 62, 219, 312, 881, 1488, 1515, 2040
SatI GCNGC 5 cut(s) 229, 408, 411, 1578, 1809
Sau3AI GATC 5 cut(s) 39, 421, 505, 541, 2185
Sau96I GGNCC 7 cut(s) 298, 367, 571, 1477, 1583, 1584, 1663
ScaI AGTACT 2 cut(s) 877, 1838
SchI GAGTC 1 cut(s) 118
ScrFI CCNGG 1 cut(s) 569
SduI GDGCHC 4 cut(s) 240, 748, 1587, 2035
SfaNI GCATC 8 cut(s) 175, 213, 422, 579, 948, 1612, 1665, 1747
SfcI CTRYAG 4 cut(s) 28, 408, 911, 1698
SfuI TTCGAA 1 cut(s) 2088
SgsI GGCGCGCC 1 cut(s) 10
SinI GGWCC 4 cut(s) 298, 367, 571, 1663
SmiMI CAYNNNNRTG 1 cut(s) 1616
SmlI CTYRAG 1 cut(s) 1772
SmoI CTYRAG 1 cut(s) 1772
SpeI ACTAGT 1 cut(s) 328
SphI GCATGC 1 cut(s) 357
SseBI AGGCCT 1 cut(s) 145
SsiI CCGC 4 cut(s) 1544, 1809, 1896, 2069
SspI AATATT 2 cut(s) 310, 772
SspMI CTAG 1 cut(s) 329
StuI AGGCCT 1 cut(s) 145
StyD4I CCNGG 1 cut(s) 567
TaaI ACNGT 3 cut(s) 174, 1702, 2030
TaiI ACGT 2 cut(s) 2018, 2246
TaqI TCGA 5 cut(s) 555, 793, 1680, 2088, 2145
TatI WGTACW 4 cut(s) 196, 397, 875, 1836
TauI GCSGC 1 cut(s) 1811
TfiI GAWTC 4 cut(s) 266, 653, 1101, 1903
Tru1I TTAA 7 cut(s) 62, 219, 312, 881, 1488, 1515, 2040
Tru9I TTAA 7 cut(s) 62, 219, 312, 881, 1488, 1515, 2040
TscAI CASTG 3 cut(s) 755, 1410, 2035
TseFI GTSAC 2 cut(s) 1390, 1705
TseI GCWGC 4 cut(s) 228, 407, 410, 1577
Tsp45I GTSAC 2 cut(s) 1390, 1705
TspRI CASTG 3 cut(s) 755, 1410, 2035
VneI GTGCAC 1 cut(s) 2031
VpaK11BI GGWCC 4 cut(s) 298, 367, 571, 1663
XapI RAATTY 5 cut(s) 76, 117, 964, 1872, 2063
XceI RCATGY 2 cut(s) 357, 1888
XmiI GTMKAC 2 cut(s) 853, 1080
XmnI GAANNNNTTC 1 cut(s) 1455
XspI CTAG 1 cut(s) 329
ZrmI AGTACT 2 cut(s) 877, 1838
Zsp2I ATGCAT 1 cut(s) 1627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.