MD13G1084500.v1.1

Microtubule-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007666
Physical Location & Seq
Reverse (-)
5914665 .. 5918868
4204 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1084500.v1.1.491

Sequence Viewer

Length: 2001 bp
ATGGCGTCTCTCTCCCGGCGAGCCTCCTGCGGCAGCTCGCCCCAGCCAACCGCCGCATCTTCCACTCCGGATCTGAAGCACCGGGTCATCACCTGCCTCAACAAGCTCGCCGACCGCGACACTCTCGCCATGGCCTCCGCCGAGCTCGACTCCATTGCCCGAACCCTCGCCCCCGACTCCTTCGCTACCTTCCTCATTTGCATTCACAACACCGACTCTTCCACCAAGCCACCCGTCCGCAAGCAATGCGTTTCGCTCCTCACCCTGCTGTCGAACTCCCACGGCGACGCCCTTGCTCCCTTTTTGTCTAAGATGATCTCCACCGTCGTCCGCCGTCTCCGCGACCCCGACTCCGTCGTCCGATCCGCTTGCGTGGACGCAGTTTCCGCCATGTCGTCCAAGATCACGACGCCGGCGTTTTCGTCGTCGTTCTTAAAGCCATTGATGGACGCTCTGGTGACGGAGCAAGACTTGAACTCTCAGATCGGCTCGGCGCTGTGTCTGGCCGGCGCGATCGATTCCGCGCCTGACCCGGACCCAGTTCAGCTGAAGCGGAGCTTGCAGCGGCTCGGGAAGCTGGCGAAGAGCGAGAGTTTCAAGGCCAAGGCGGCACTGCTGGTGCTTGTTGGGAGCATTGTTGGTGCCGGTGGCGCGTCGAGCCAAGGCGCGTTGGACTGGCTTGTGCCGTGCGGGCTTGAGTTCTTGAGCAGCGAGGACTGGACAGTGAGGAAGGCCGCGGCCGAGGCGCTTGGGAAGGTGGCTACCGTGGAGACAGATTTGGCGCCGCTTTATAAAGCTTTGTGCTTGAATGCTTTGGAAAGTCGGAGATTTGATAAGGTGAAGCTAGTCCGGGAGACTATGAACCAGACATTGGAGTTGTGGAAGGTTCTTCTTGCCGGCGATTCCGAAGAGATTCCTGCTCCAATTCAATCCAGATCGTCAACTGATAATGGGATTGATCGATGCTCTCCTCCCTCATTGAAAAGTTCCGATGATGCCGGTTTTAGGACTCCTCAACCAAAGAAAGCAGTCCCCACAAACAGGTCCCCTCCATCGGATACTTCTTTGGTGACCACACCTAAAAAGGGAAGTCCTTCAAGTGGAAGCGATAGAAAACCAGACGCAGCTGTGTTCGCTAAGCAGGACCACAAGAAACCCTCTGATTGGAAAATTGAAATTGCTACGCCAAACTCCCTCTCTTTGACGGTGACTCATAAGGATGATATTGTTAGGAGCGACTGTAGAGATCCGGATTCAGGAAAGAATGACCACTTTGGAAATGGCAAGCCAGAAACTAAGCACTTGCTCTTTAGTAAGAATCATGGTGAAAAAGTACACAGATTTGGTGGTTTAAAATCTGGGTCACGCGTTGTTCCATTTAATGATGAGGAGAACTATGACTCAGATATTGTAGTCGGAAATGCTGCAGAAGAAGTTTATGAGAACCAAAAGGATGCTGAGGATCTCAGTTTGATCCGCGAACAACTTATTCAGATTGAAAACCAGCAGTCCAGTTTATTAAACCTACTCCAGAGATTTATTGGGAGCTCTCAGAGTGGGTTGAATGCTCTTGAGTCACGTGTACACGGCCTTGAGATGGCTCTTGATGAAATATCATATGATTTGGCAATATCAAGTGGAAGGATCCCAAACACAGAATCTGCAGAAAATACATGCTGCAAGCTGCCTGGTGCAGAATTCTTGAGTTCCAAGTTCTGGCGTAGAACAGATGGGCGGTGTACTACTTCAAGGCTCACTTCTGGAACCATTCCCTCACTCAACGCTGTGCCTAACATACCTAATAAAAATACAAGTACTGAATCGTGTGCTTTTGACCGTGAAAGGTTTCAGCACCAAAGTAGGGGTGCATTTACTGGTTACTCGGTGGCAGAAATCCAAGATGCTGAGAGGGTTCAAGTTTCCAATGCTAGTCGTTATGATGGGGTATCGCCAACTACTTGTACACCATCAAGGAACATCAACAAAAGATCATCTGCTTAA

Protein Analysis

667

Amino Acids

71.85

Weight (kDa)

7.5

Isoelectric Point (pI)

54.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOR1L1_N PF24714 24 - 295 3e-114 TORTIFOLIA1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 792
AarI CACCTGC 1 cut(s) 101
AasI GACNNNNNNGTC 1 cut(s) 356
Acc36I ACCTGC 1 cut(s) 101
AccB1I GGYRCC 2 cut(s) 641, 781
AccB7I CCANNNNNTGG 2 cut(s) 871, 1716
AccII CGCG 9 cut(s) 117, 342, 512, 524, 653, 668, 737, 1368, 1479
AccIII TCCGGA 2 cut(s) 67, 1249
AclWI GGATC 7 cut(s) 78, 357, 1241, 1468, 1470, 1639, 1652
AcoI YGGCCR 2 cut(s) 504, 738
AcsI RAATTY 1 cut(s) 1697
AcuI CTGAAG 2 cut(s) 95, 569
AcvI CACGTG 1 cut(s) 1580
AcyI GRCGYC 4 cut(s) 5, 288, 410, 782
AfaI GTAC 5 cut(s) 1335, 1584, 1741, 1816, 1963
AflIII ACRYGT 2 cut(s) 1366, 1579
AjnI CCWGG 1 cut(s) 1687
AjuI GAANNNNNNNTTGG 2 cut(s) 854, 886
Alw21I GWGCWC 2 cut(s) 147, 1550
Alw26I GTCTC 4 cut(s) 12, 341, 764, 848
AlwI GGATC 7 cut(s) 78, 357, 1241, 1468, 1470, 1639, 1652
AlwNI CAGNNNCTG 1 cut(s) 1661
Ama87I CYCGRG 1 cut(s) 569
Aor13HI TCCGGA 2 cut(s) 67, 1249
AoxI GGCC 6 cut(s) 132, 504, 600, 732, 738, 1588
ApeKI GCWGC 7 cut(s) 33, 562, 708, 1124, 1424, 1677, 1684
ApoI RAATTY 1 cut(s) 1697
Asp700I GAANNNNTTC 3 cut(s) 912, 1093, 1845
AspLEI GCGC 7 cut(s) 496, 512, 526, 653, 668, 748, 784
AspS9I GGNCC 3 cut(s) 535, 1044, 1144
AsuC2I CCSGG 4 cut(s) 16, 83, 533, 851
AsuHPI GGTGA 7 cut(s) 82, 253, 469, 850, 1081, 1219, 1337
AvaI CYCGRG 1 cut(s) 569
AvaII GGWCC 3 cut(s) 535, 1044, 1144
BamHI GGATCC 1 cut(s) 1644
BanI GGYRCC 2 cut(s) 641, 781
BanII GRGCYC 2 cut(s) 147, 1550
BbrPI CACGTG 1 cut(s) 1580
Bbv12I GWGCWC 2 cut(s) 147, 1550
BbvCI CCTCAGC 1 cut(s) 1458
BbvI GCAGC 7 cut(s) 45, 574, 720, 1136, 1411, 1664, 1671
BccI CCATC 6 cut(s) 439, 1060, 1591, 1724, 1934, 1975
BceAI ACGGC 4 cut(s) 298, 318, 670, 1603
BcgI CGANNNNNNTGC 8 cut(s) 9, 43, 137, 171, 275, 309, 351, 385
BciT130I CCWGG 1 cut(s) 1689
BciVI GTATCC 1 cut(s) 1051
BcnI CCSGG 4 cut(s) 16, 83, 533, 851
BcoDI GTCTC 4 cut(s) 12, 341, 764, 848
BfaI CTAG 2 cut(s) 845, 1929
BfmI CTRYAG 3 cut(s) 1240, 1425, 1662
BfoI RGCGCY 3 cut(s) 497, 749, 785
BfuAI ACCTGC 1 cut(s) 101
BfuI GTATCC 1 cut(s) 1051
BglI GCCNNNNNGGC 2 cut(s) 608, 691
BlpI GCTNAGC 1 cut(s) 1137
BmcAI AGTACT 1 cut(s) 1816
Bme1390I CCNGG 5 cut(s) 16, 83, 533, 851, 1689
Bme18I GGWCC 3 cut(s) 535, 1044, 1144
BmeT110I CYCGRG 1 cut(s) 569
BmgT120I GGNCC 3 cut(s) 535, 1044, 1144
BmiI GGNNCC 6 cut(s) 537, 643, 783, 1046, 1646, 1765
BmrFI CCNGG 5 cut(s) 16, 83, 533, 851, 1689
BmrI ACTGGG 1 cut(s) 533
BmsI GCATC 5 cut(s) 65, 953, 985, 1444, 1891
BmuI ACTGGG 1 cut(s) 533
BplI GAGNNNNNCTC 2 cut(s) 134, 166
BpmI CTGGAG 1 cut(s) 1514
Bpu10I CCTNAGC 1 cut(s) 1458
Bpu1102I GCTNAGC 1 cut(s) 1137
BpuEI CTTGAG 5 cut(s) 716, 724, 1592, 1613, 1723
BpuMI CCSGG 4 cut(s) 16, 83, 533, 851
Bsa29I ATCGAT 2 cut(s) 516, 961
BsaAI YACGTR 1 cut(s) 1580
BsaHI GRCGYC 4 cut(s) 5, 288, 410, 782
BsaJI CCNNGG 7 cut(s) 129, 280, 603, 661, 735, 741, 765
BsaWI WCCGGW 2 cut(s) 67, 1249
BsaXI ACNNNNNCTCC 4 cut(s) 335, 365, 1225, 1255
Bse118I RCCGGY 5 cut(s) 412, 506, 644, 896, 998
Bse1I ACTGG 5 cut(s) 539, 680, 722, 1512, 1879
Bse3DI GCAATG 2 cut(s) 153, 251
BseAI TCCGGA 2 cut(s) 67, 1249
BseBI CCWGG 1 cut(s) 1689
BseCI ATCGAT 2 cut(s) 516, 961
BseDI CCNNGG 7 cut(s) 129, 280, 603, 661, 735, 741, 765
BseGI GGATG 2 cut(s) 1225, 1459
BseMI GCAATG 2 cut(s) 153, 251
BseMII CTCAG 6 cut(s) 494, 1416, 1449, 1480, 1565, 1896
BseNI ACTGG 5 cut(s) 539, 680, 722, 1512, 1879
BseRI GAGGAG 4 cut(s) 248, 960, 1002, 1403
BseX3I CGGCCG 1 cut(s) 738
BseXI GCAGC 7 cut(s) 45, 574, 720, 1136, 1411, 1664, 1671
BseYI CCCAGC 1 cut(s) 42
BsgI GTGCAG 1 cut(s) 1713
Bsh1236I CGCG 9 cut(s) 117, 342, 512, 524, 653, 668, 737, 1368, 1479
Bsh1285I CGRYCG 3 cut(s) 115, 516, 741
BshFI GGCC 6 cut(s) 134, 506, 602, 734, 740, 1590
BshNI GGYRCC 2 cut(s) 641, 781
BshVI ATCGAT 2 cut(s) 516, 961
BsiEI CGRYCG 3 cut(s) 115, 516, 741
BsiHKAI GWGCWC 2 cut(s) 147, 1550
BsiHKCI CYCGRG 1 cut(s) 569
BslFI GGGAC 2 cut(s) 1016, 1030
BsmAI GTCTC 4 cut(s) 12, 341, 764, 848
BsmBI CGTCTC 2 cut(s) 12, 341
BsmFI GGGAC 2 cut(s) 1016, 1030
BsmI GAATGC 3 cut(s) 201, 814, 1570
BsnI GGCC 6 cut(s) 134, 506, 602, 734, 740, 1590
BsoBI CYCGRG 1 cut(s) 569
Bsp1286I GDGCHC 2 cut(s) 147, 1550
Bsp13I TCCGGA 2 cut(s) 67, 1249
Bsp1407I TGTACA 2 cut(s) 1582, 1961
Bsp1720I GCTNAGC 1 cut(s) 1137
Bsp19I CCATGG 1 cut(s) 129
BspANI GGCC 6 cut(s) 134, 506, 602, 734, 740, 1590
BspCNI CTCAG 6 cut(s) 493, 1415, 1450, 1479, 1564, 1897
BspDI ATCGAT 2 cut(s) 516, 961
BspEI TCCGGA 2 cut(s) 67, 1249
BspFNI CGCG 9 cut(s) 117, 342, 512, 524, 653, 668, 737, 1368, 1479
BspLI GGNNCC 6 cut(s) 537, 643, 783, 1046, 1646, 1765
BspMAI CTGCAG 2 cut(s) 1429, 1666
BspMI ACCTGC 1 cut(s) 101
BspPI GGATC 7 cut(s) 78, 357, 1241, 1468, 1470, 1639, 1652
BspQI GCTCTTC 1 cut(s) 578
BspT107I GGYRCC 2 cut(s) 641, 781
BsrDI GCAATG 2 cut(s) 153, 251
BsrFI RCCGGY 5 cut(s) 412, 506, 644, 896, 998
BsrGI TGTACA 2 cut(s) 1582, 1961
BsrI ACTGG 5 cut(s) 539, 680, 722, 1512, 1879
BssAI RCCGGY 5 cut(s) 412, 506, 644, 896, 998
BssECI CCNNGG 7 cut(s) 129, 280, 603, 661, 735, 741, 765
BssNI GRCGYC 4 cut(s) 5, 288, 410, 782
BssT1I CCWWGG 3 cut(s) 129, 603, 661
Bst2UI CCWGG 1 cut(s) 1689
Bst4CI ACNGT 6 cut(s) 325, 724, 766, 1207, 1241, 1838
Bst6I CTCTTC 3 cut(s) 223, 578, 903
BstACI GRCGYC 4 cut(s) 5, 288, 410, 782
BstAPI GCANNNNNTGC 1 cut(s) 246
BstAUI TGTACA 2 cut(s) 1582, 1961
BstBAI YACGTR 1 cut(s) 1580
BstDEI CTNAG 9 cut(s) 309, 480, 1137, 1296, 1402, 1458, 1466, 1551, 1905
BstDSI CCRYGG 4 cut(s) 129, 280, 735, 765
BstEII GGTNACC 1 cut(s) 1069
BstF5I GGATG 2 cut(s) 1225, 1459
BstFNI CGCG 9 cut(s) 117, 342, 512, 524, 653, 668, 737, 1368, 1479
BstH2I RGCGCY 3 cut(s) 497, 749, 785
BstHHI GCGC 7 cut(s) 496, 512, 526, 653, 668, 748, 784
BstMAI GTCTC 4 cut(s) 12, 341, 764, 848
BstMCI CGRYCG 3 cut(s) 115, 516, 741
BstNI CCWGG 1 cut(s) 1689
BstNSI RCATGY 1 cut(s) 1677
BstPI GGTNACC 1 cut(s) 1069
BstSCI CCNGG 5 cut(s) 14, 81, 531, 849, 1687
BstSFI CTRYAG 3 cut(s) 1240, 1425, 1662
BstUI CGCG 9 cut(s) 117, 342, 512, 524, 653, 668, 737, 1368, 1479
BstV1I GCAGC 7 cut(s) 45, 574, 720, 1136, 1411, 1664, 1671
BstX2I RGATCY 4 cut(s) 70, 1246, 1462, 1644
BstYI RGATCY 4 cut(s) 70, 1246, 1462, 1644
BstZI CGGCCG 1 cut(s) 738
Bsu15I ATCGAT 2 cut(s) 516, 961
BsuI GTATCC 1 cut(s) 1051
BsuRI GGCC 6 cut(s) 134, 506, 602, 734, 740, 1590
BsuTUI ATCGAT 2 cut(s) 516, 961
BtgI CCRYGG 4 cut(s) 129, 280, 735, 765
BtsCI GGATG 2 cut(s) 1225, 1459
BtsI GCAGTG 1 cut(s) 611
BtsIMutI CAGTG 2 cut(s) 611, 729
BveI ACCTGC 1 cut(s) 101
CaiI CAGNNNCTG 1 cut(s) 1661
CfoI GCGC 7 cut(s) 496, 512, 526, 653, 668, 748, 784
Cfr10I RCCGGY 5 cut(s) 412, 506, 644, 896, 998
Cfr13I GGNCC 3 cut(s) 535, 1044, 1144
Cfr42I CCGCGG 1 cut(s) 738
ClaI ATCGAT 2 cut(s) 516, 961
CseI GACGC 6 cut(s) 296, 386, 418, 458, 642, 1130
Csp6I GTAC 5 cut(s) 1334, 1583, 1740, 1815, 1962
CviAII CATG 4 cut(s) 130, 391, 1322, 1674
CviQI GTAC 5 cut(s) 1334, 1583, 1740, 1815, 1962
DdeI CTNAG 9 cut(s) 309, 480, 1137, 1296, 1402, 1458, 1466, 1551, 1905
DinI GGCGCC 1 cut(s) 783
DraI TTTAAA 1 cut(s) 1353
DrdI GACNNNNNNGTC 1 cut(s) 356
DseDI GACNNNNNNGTC 1 cut(s) 356
EaeI YGGCCR 2 cut(s) 504, 738
EagI CGGCCG 1 cut(s) 738
Eam1104I CTCTTC 3 cut(s) 223, 578, 903
EarI CTCTTC 3 cut(s) 223, 578, 903
EciI GGCGGA 3 cut(s) 127, 320, 376
Ecl136II GAGCTC 2 cut(s) 145, 1548
EclXI CGGCCG 1 cut(s) 738
Eco130I CCWWGG 3 cut(s) 129, 603, 661
Eco24I GRGCYC 2 cut(s) 147, 1550
Eco47I GGWCC 3 cut(s) 535, 1044, 1144
Eco52I CGGCCG 1 cut(s) 738
Eco53kI GAGCTC 2 cut(s) 145, 1548
Eco57I CTGAAG 2 cut(s) 95, 569
Eco72I CACGTG 1 cut(s) 1580
Eco88I CYCGRG 1 cut(s) 569
Eco91I GGTNACC 1 cut(s) 1069
EcoICRI GAGCTC 2 cut(s) 145, 1548
EcoO109I RGGNCCY 1 cut(s) 1044
EcoO65I GGTNACC 1 cut(s) 1069
EcoRI GAATTC 1 cut(s) 1697
EcoRII CCWGG 1 cut(s) 1687
EcoT14I CCWWGG 3 cut(s) 129, 603, 661
EcoT38I GRGCYC 2 cut(s) 147, 1550
EgeI GGCGCC 1 cut(s) 783
EheI GGCGCC 1 cut(s) 783
ErhI CCWWGG 3 cut(s) 129, 603, 661
Esp3I CGTCTC 2 cut(s) 12, 341
FaeI CATG 4 cut(s) 133, 394, 1325, 1677
FalI AAGNNNNNCTT 4 cut(s) 542, 574, 1741, 1773
FaqI GGGAC 2 cut(s) 1016, 1030
FatI CATG 4 cut(s) 129, 390, 1321, 1673
FauI CCCGC 1 cut(s) 683
FauNDI CATATG 1 cut(s) 1618
FokI GGATG 2 cut(s) 1232, 1466
FriOI GRGCYC 2 cut(s) 147, 1550
FspBI CTAG 2 cut(s) 845, 1929
GlaI GCGC 7 cut(s) 495, 511, 525, 652, 667, 747, 783
GsaI CCCAGC 1 cut(s) 46
GsuI CTGGAG 1 cut(s) 1514
HaeII RGCGCY 3 cut(s) 497, 749, 785
HaeIII GGCC 6 cut(s) 134, 506, 602, 734, 740, 1590
HgaI GACGC 6 cut(s) 296, 386, 418, 458, 642, 1130
HhaI GCGC 7 cut(s) 496, 512, 526, 653, 668, 748, 784
Hin1I GRCGYC 4 cut(s) 5, 288, 410, 782
Hin1II CATG 4 cut(s) 133, 394, 1325, 1677
Hin6I GCGC 7 cut(s) 494, 510, 524, 651, 666, 746, 782
HinP1I GCGC 7 cut(s) 494, 510, 524, 651, 666, 746, 782
HincII GTYRAC 1 cut(s) 942
HindII GTYRAC 1 cut(s) 942
HindIII AAGCTT 1 cut(s) 795
HphI GGTGA 7 cut(s) 82, 253, 469, 850, 1081, 1219, 1337
Hpy166II GTNNAC 7 cut(s) 376, 942, 1336, 1583, 1585, 1740, 1964
Hpy8I GTNNAC 7 cut(s) 376, 942, 1336, 1583, 1585, 1740, 1964
Hpy99I CGWCG 7 cut(s) 290, 329, 359, 412, 427, 430, 658
HpyAV CCTTC 7 cut(s) 190, 199, 724, 748, 877, 1104, 1635
HpyCH4III ACNGT 6 cut(s) 325, 724, 766, 1207, 1241, 1838
HpyCH4IV ACGT 1 cut(s) 1579
HpyCH4V TGCA 7 cut(s) 201, 562, 1427, 1664, 1680, 1694, 1868
HpyF3I CTNAG 9 cut(s) 309, 480, 1137, 1296, 1402, 1458, 1466, 1551, 1905
HpySE526I ACGT 1 cut(s) 1579
Hsp92I GRCGYC 4 cut(s) 5, 288, 410, 782
Hsp92II CATG 4 cut(s) 133, 394, 1325, 1677
HspAI GCGC 7 cut(s) 494, 510, 524, 651, 666, 746, 782
KasI GGCGCC 1 cut(s) 781
Kpn2I TCCGGA 2 cut(s) 67, 1249
KroI GCCGGC 3 cut(s) 412, 506, 896
KroNI GCCGGC 3 cut(s) 414, 508, 898
KspI CCGCGG 1 cut(s) 738
LguI GCTCTTC 1 cut(s) 578
LmnI GCTCC 8 cut(s) 261, 301, 463, 555, 630, 925, 1233, 1545
Lsp1109I GCAGC 7 cut(s) 45, 574, 720, 1136, 1411, 1664, 1671
LweI GCATC 5 cut(s) 65, 953, 985, 1444, 1891
MaeI CTAG 2 cut(s) 845, 1929
MaeII ACGT 1 cut(s) 1579
MaeIII GTNAC 6 cut(s) 457, 1069, 1207, 1362, 1575, 1877
MboII GAAGA 6 cut(s) 51, 210, 595, 881, 920, 1442
MflI RGATCY 4 cut(s) 70, 1246, 1462, 1644
MhlI GDGCHC 2 cut(s) 147, 1550
MluCI AATT 4 cut(s) 924, 1170, 1176, 1697
MluI ACGCGT 1 cut(s) 1366
Mly113I GGCGCC 1 cut(s) 782
MlyI GAGTC 8 cut(s) 143, 170, 209, 344, 1003, 1204, 1394, 1583
MmeI TCCRAC 3 cut(s) 651, 803, 1396
MreI CGCCGGCG 1 cut(s) 412
MroI TCCGGA 2 cut(s) 67, 1249
MroNI GCCGGC 3 cut(s) 412, 506, 896
MroXI GAANNNNTTC 3 cut(s) 912, 1093, 1845
MseI TTAA 5 cut(s) 434, 1352, 1380, 1520, 1999
MslI CAYNNNNRTG 1 cut(s) 1218
MspA1I CMGCKG 4 cut(s) 547, 565, 737, 1127
MspR9I CCNGG 5 cut(s) 16, 83, 533, 851, 1689
Mva1269I GAATGC 3 cut(s) 201, 814, 1570
MvaI CCWGG 1 cut(s) 1689
MvnI CGCG 9 cut(s) 117, 342, 512, 524, 653, 668, 737, 1368, 1479
NaeI GCCGGC 3 cut(s) 414, 508, 898
NarI GGCGCC 1 cut(s) 782
NciI CCSGG 4 cut(s) 16, 83, 533, 851
NcoI CCATGG 1 cut(s) 129
NdeI CATATG 1 cut(s) 1618
NgoMIV GCCGGC 3 cut(s) 412, 506, 896
NlaIII CATG 4 cut(s) 133, 394, 1325, 1677
NlaIV GGNNCC 6 cut(s) 537, 643, 783, 1046, 1646, 1765
NmeAIII GCCGAG 3 cut(s) 166, 470, 766
NmuCI GTSAC 5 cut(s) 457, 1069, 1207, 1362, 1575
NspI RCATGY 1 cut(s) 1677
PaqCI CACCTGC 1 cut(s) 101
PciSI GCTCTTC 1 cut(s) 578
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 413
PctI GAATGC 3 cut(s) 201, 814, 1570
PdiI GCCGGC 3 cut(s) 414, 508, 898
PdmI GAANNNNTTC 3 cut(s) 912, 1093, 1845
PfeI GAWTC 7 cut(s) 518, 902, 913, 1253, 1318, 1658, 1820
PflFI GACNNNGTC 1 cut(s) 353
PflMI CCANNNNNTGG 2 cut(s) 871, 1716
PfoI TCCNGGA 1 cut(s) 849
Ple19I CGATCG 1 cut(s) 516
PleI GAGTC 8 cut(s) 143, 170, 209, 344, 1003, 1204, 1394, 1582
PluTI GGCGCC 1 cut(s) 785
PmaCI CACGTG 1 cut(s) 1580
PmlI CACGTG 1 cut(s) 1580
PpsI GAGTC 8 cut(s) 143, 170, 209, 344, 1003, 1204, 1394, 1582
Ppu21I YACGTR 1 cut(s) 1580
PpuMI RGGWCCY 1 cut(s) 1044
PsiI TTATAA 1 cut(s) 792
Psp124BI GAGCTC 2 cut(s) 147, 1550
Psp5II RGGWCCY 1 cut(s) 1044
Psp6I CCWGG 1 cut(s) 1687
PspCI CACGTG 1 cut(s) 1580
PspEI GGTNACC 1 cut(s) 1069
PspFI CCCAGC 1 cut(s) 42
PspGI CCWGG 1 cut(s) 1687
PspN4I GGNNCC 6 cut(s) 537, 643, 783, 1046, 1646, 1765
PspPI GGNCC 3 cut(s) 535, 1044, 1144
PspPPI RGGWCCY 1 cut(s) 1044
PstI CTGCAG 2 cut(s) 1429, 1666
PstNI CAGNNNCTG 1 cut(s) 1661
PsuI RGATCY 4 cut(s) 70, 1246, 1462, 1644
PsyI GACNNNGTC 1 cut(s) 353
PvuI CGATCG 1 cut(s) 516
PvuII CAGCTG 2 cut(s) 547, 1127
RsaI GTAC 5 cut(s) 1335, 1584, 1741, 1816, 1963
RsaNI GTAC 5 cut(s) 1334, 1583, 1740, 1815, 1962
RseI CAYNNNNRTG 1 cut(s) 1218
SacI GAGCTC 2 cut(s) 147, 1550
SacII CCGCGG 1 cut(s) 738
SapI GCTCTTC 1 cut(s) 578
SaqAI TTAA 5 cut(s) 434, 1352, 1380, 1520, 1999
Sau96I GGNCC 3 cut(s) 535, 1044, 1144
ScaI AGTACT 1 cut(s) 1816
SchI GAGTC 8 cut(s) 143, 170, 209, 344, 1003, 1204, 1394, 1583
ScrFI CCNGG 5 cut(s) 16, 83, 533, 851, 1689
SduI GDGCHC 2 cut(s) 147, 1550
SfaNI GCATC 5 cut(s) 65, 953, 985, 1444, 1891
SfcI CTRYAG 3 cut(s) 1240, 1425, 1662
SfoI GGCGCC 1 cut(s) 783
Sfr303I CCGCGG 1 cut(s) 738
SgrAI CRCCGGYG 1 cut(s) 412
SgrBI CCGCGG 1 cut(s) 738
SinI GGWCC 3 cut(s) 535, 1044, 1144
SmiMI CAYNNNNRTG 1 cut(s) 1218
SmlI CTYRAG 5 cut(s) 695, 703, 1571, 1592, 1702
SmoI CTYRAG 5 cut(s) 695, 703, 1571, 1592, 1702
Sse9I AATT 4 cut(s) 924, 1170, 1176, 1697
SspDI GGCGCC 1 cut(s) 781
SspMI CTAG 2 cut(s) 845, 1929
SstI GAGCTC 2 cut(s) 147, 1550
StyD4I CCNGG 5 cut(s) 14, 81, 531, 849, 1687
StyI CCWWGG 3 cut(s) 129, 603, 661
TaaI ACNGT 6 cut(s) 325, 724, 766, 1207, 1241, 1838
TaiI ACGT 1 cut(s) 1582
TaqI TCGA 5 cut(s) 147, 272, 516, 656, 961
TasI AATT 4 cut(s) 924, 1170, 1176, 1697
TatI WGTACW 5 cut(s) 1333, 1582, 1739, 1814, 1961
TauI GCSGC 7 cut(s) 33, 56, 568, 611, 737, 740, 787
TfiI GAWTC 7 cut(s) 518, 902, 913, 1253, 1318, 1658, 1820
Tru1I TTAA 5 cut(s) 434, 1352, 1380, 1520, 1999
Tru9I TTAA 5 cut(s) 434, 1352, 1380, 1520, 1999
TscAI CASTG 2 cut(s) 618, 729
TseFI GTSAC 5 cut(s) 457, 1069, 1207, 1362, 1575
TseI GCWGC 7 cut(s) 33, 562, 708, 1124, 1424, 1677, 1684
Tsp45I GTSAC 5 cut(s) 457, 1069, 1207, 1362, 1575
TspDTI ATGAA 2 cut(s) 875, 1623
TspGWI ACGGA 2 cut(s) 343, 476
TspRI CASTG 2 cut(s) 618, 729
Tth111I GACNNNGTC 1 cut(s) 353
Van91I CCANNNNNTGG 2 cut(s) 871, 1716
VpaK11BI GGWCC 3 cut(s) 535, 1044, 1144
XapI RAATTY 1 cut(s) 1697
XceI RCATGY 1 cut(s) 1677
XcmI CCANNNNNNNNNTGG 2 cut(s) 1277, 1538
XmnI GAANNNNTTC 3 cut(s) 912, 1093, 1845
XspI CTAG 2 cut(s) 845, 1929
ZrmI AGTACT 1 cut(s) 1816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.