Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007666

Physical Location & Seq

Genomic Coordinates Reverse (-)

5852188 .. 5855856

Gene Structure 3669 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1083600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2004 bp

ATGGCGTCTCTCTCCCGGCGAGCCTCCTTCGGCAGCTCGCCCCAACCAACCGCCGGATCTTCCACGCCGGATCTGAAGCACCGCGTCATCACCTGCCTCAACAAGCTCGCCGACCGCGACACTCTCGCCATGGCCTCCGCCGAGCTCGACTCCATTGCCCGAACCCTCGCCCCCGGCTTCTTCGCTACCTTCCTCATCTGCATTCACAACACCGACTCTTCCACCAAGCCGCCCGTCCGCAAGCAATGCGTTTCTCTCCTCACCCTGCTGTCGAACTCCCACGGCGACGCCCTCGCTCCGTTTTTGTCCAAAATGATCTCCACCGTCGTCCGCCGTCTCCGCGACCCCGACTCCGTCGTCCGATCCGCTTGCGTGGAGGCAGTTTCCGCCATGTCGTCCAAGATCACTACACCGGCGTTTTCGTCGTCATTCTTAAAGCCATTGATGGACACTCTGGCGACGGAGCAAGACTTGAATTCTCAGATCGGCTCGGCGCTGTGTCTGGCTGCCGCGATCGATTCTGCGCCTGACCCGGACCCGGTTCAGCTGAAGCGGAGCCTGCAGCGGCTCTCGAAGCTGGCGAAGAGCGAGAGCTTCAAGGCCAAGGCGGCGCTACTGGTGCTTGTTGGGAGCATTATCGGGGCTGGTGGCGCGTCGAGCCAAGGCTCGTTGGACTGGCTTGTGCCGTGCGTGATTGAGTTTTTGAGCAGCGAGGACTGGACAACGAGGAAGGCCGCGGCCGAGGCGCTTGGGAAGGTGGCTACGGTGGAGACAGATTTGGCGCCGATTTATAAAGCGTCGTGCTTGAATGCCTTGGAAAGTCGGAGATTTGATAAGGTGAAGTTAGTCCGGGAAACTATGAACGAGACATTGGAGCTGTGGAAGGTTCTTCTTGCCGGCGATTCCGAAGCTGTTCCTGCTCCAATTCAATCCAGATCTTCAACTGATAATGGGATTGGTCGATGCTCTCCTCCCTCATCAAAAAGTTCCGATGATGCCGGTTGTTGTAGGATTCCTCAACCAAAGAAAACAGTCCCCACAAACAGGTCCCCTCCATCAGATGCTTCTTTGGTGACCACAGCTAAAAAGGGAAGTCCTTTAAGTAGAAACGATAGAAAACCAGACACTGCCGTGTTCTCGAAGCAGGACCACAAGAAACCCACCGATTGGAAATTTGAAATTGCTACGCCAAACTCTCTCTCCTCAAAGGTGACCCATAAGGATGATATTGTTAGGAGTGACCCTAGAGATCCGGAATCAGGAAAGAATGACCCCATTGGAAATGGCGTGCTAGAAACTAACAGCTTACTCTTTAGTAAGAATCCTGGTGAAAAAGTATACAGATCTGGTGGTTTAAAATCCGGGTCACTCGTTGTTCCATTTAATGATCATGAGAACTATGACTCAGATGTTGAAGTCAGCAATGATGCAGAAGAAGTTTATGAGAAACTAAAGGATGCTGAGGATCTCAGTTTGATCCGCGAACAACTTATTCAGATTGAAAACCAGCAGTCCAGTTTATTAAACCTACTCCAGAGATTTATTGGGAGCTCTCAGAGTGGGTTGAATGCTCTTGAGTCACGTGTGCACGGCCTTGAGATGGCTCTAGATGAAATATCTTACGATTTGGCAATATCAAGTGGAAGGATCCCAAACGCAGAATCTGCCGAAAATACCTGCTGCAAGCTGCCGGGTGCAGAATTCTTGAGTTCCAAGTTCTGGCGTAGAACAGATGGGAGGTGTTCTACTTCGAGGCTCACTTCTGGAACCATTCCCTCACTTAATGCTATGCCTAACATAGCTAATAAAAATACAAGTGCTGAATCGTGTACTTTTGACCGCGAAAGGTTTCAGCACCAAAGTAGAAGTGCATCTACCGGTCACTCGTTGGCAAAAATGCAAGATGCCGAGAGGGTTCAGGCTTGCAATGCTAGTCGGTATGACGGGGTTTCGCCAGCTACATGTACATCATCAAGGAACCTGAACAAAAGACCATCTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005874 GO:0008017 GO:0008092 GO:0015630 GO:0015631 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0099080 GO:0099081 GO:0099512 GO:0099513

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cohesin_HEAT HEAT

Protein Analysis

668

Amino Acids

71.86

Weight (kDa)

8.23

Isoelectric Point (pI)

56.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TOR1L1_N	PF24714	24 - 295	1.3e-115	TORTIFOLIA1 N-terminal
HEAT_EF3_N	PF24987	69 - 177	4.7e-06	EF3 N-terminal HEAT repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007666)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G27210 AT1G59850
fragaria_vesca	FvH4_4g35310 FvH4_4g35310 FvH4_4g35310
malus_domestica	MD13G1084500.v1.1 MD16G1083600.v1.1
prunus_persica	Prupe.1G262400_v2.0.a1
pyrus_communis	pycom13g07290 pycom16g07250
rosa_chinensis	RchiOBHm_Chr4g0444931
rosa_laevigata	RLG00000005786
rosa_multiflora	Rmu_co8293673.1_g000001 Rmu_sc0010689.1_g000001 Rmu_sc0011561.1_g000001 Rmu_sc0026285.1_g000001
rosa_roxburghii	Rroxscaffold_5G00385420
rosa_rugosa	Rorug04G0356500 Rorug04G0356500
rosa_samantha	Rh4AG418000 Rh4BG429700 Rh4CG444500 Rh4DG425700
rosa_wichuraiana	Rw4G035820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	792
AarI	CACCTGC	1 cut(s)	101
AasI	GACNNNNNNGTC	1 cut(s)	356
Acc36I	ACCTGC	2 cut(s)	101, 1685
AccB1I	GGYRCC	1 cut(s)	781
AccB7I	CCANNNNNTGG	2 cut(s)	1167, 1719
AccI	GTMKAC	1 cut(s)	1338
AccII	CGCG	8 cut(s)	84, 117, 342, 512, 653, 737, 1482, 1842
AccIII	TCCGGA	1 cut(s)	1252
AclWI	GGATC	8 cut(s)	64, 78, 357, 1244, 1471, 1473, 1642, 1655
AcoI	YGGCCR	1 cut(s)	738
AcsI	RAATTY	3 cut(s)	475, 1172, 1700
AcuI	CTGAAG	2 cut(s)	95, 569
AcvI	CACGTG	1 cut(s)	1583
AcyI	GRCGYC	3 cut(s)	5, 288, 782
AfaI	GTAC	2 cut(s)	1831, 1966
AfiI	CCNNNNNNNGG	7 cut(s)	53, 538, 1044, 1167, 1259, 1600, 1719
AflIII	ACRYGT	2 cut(s)	1582, 1961
AgeI	ACCGGT	1 cut(s)	1877
AgsI	TTSAA	9 cut(s)	475, 598, 808, 929, 942, 1178, 1415, 1502, 1567
AjnI	CCWGG	1 cut(s)	1324
AjuI	GAANNNNNNNTTGG	2 cut(s)	854, 886
AleI	CACNNNNGTG	1 cut(s)	1130
Alw21I	GWGCWC	3 cut(s)	147, 1553, 1590
Alw26I	GTCTC	4 cut(s)	12, 341, 764, 860
Alw44I	GTGCAC	1 cut(s)	1586
AlwI	GGATC	8 cut(s)	64, 78, 357, 1244, 1471, 1473, 1642, 1655
AlwNI	CAGNNNCTG	2 cut(s)	1127, 1664
Aor13HI	TCCGGA	1 cut(s)	1252
AoxI	GGCC	5 cut(s)	132, 600, 732, 738, 1591
ApaLI	GTGCAC	1 cut(s)	1586
ApeKI	GCWGC	6 cut(s)	33, 506, 562, 708, 1680, 1687
ApoI	RAATTY	3 cut(s)	475, 1172, 1700
AsiGI	ACCGGT	1 cut(s)	1877
Asp700I	GAANNNNTTC	2 cut(s)	912, 1848
AspLEI	GCGC	6 cut(s)	496, 526, 613, 653, 748, 784
AspS9I	GGNCC	3 cut(s)	535, 1047, 1147
AsuC2I	CCSGG	7 cut(s)	16, 174, 533, 539, 851, 1363, 1692
AsuHPI	GGTGA	6 cut(s)	82, 253, 850, 1084, 1222, 1340
AvaII	GGWCC	3 cut(s)	535, 1047, 1147
BaeGI	GKGCMC	1 cut(s)	1590
BamHI	GGATCC	1 cut(s)	1647
BanI	GGYRCC	1 cut(s)	781
BanII	GRGCYC	2 cut(s)	147, 1553
BarI	GAAGNNNNNNTAC	2 cut(s)	1858, 1890
BbrPI	CACGTG	1 cut(s)	1583
Bbv12I	GWGCWC	3 cut(s)	147, 1553, 1590
BbvCI	CCTCAGC	1 cut(s)	1461
BbvI	GCAGC	6 cut(s)	45, 493, 574, 720, 1667, 1674
BccI	CCATC	5 cut(s)	439, 1063, 1594, 1727, 2002
BceAI	ACGGC	5 cut(s)	298, 318, 670, 1115, 1606
BcgI	CGANNNNNNTGC	4 cut(s)	137, 171, 351, 385
BciT130I	CCWGG	1 cut(s)	1326
BclI	TGATCA	1 cut(s)	1387
BcnI	CCSGG	7 cut(s)	16, 174, 533, 539, 851, 1363, 1692
BcoDI	GTCTC	4 cut(s)	12, 341, 764, 860
BfaI	CTAG	4 cut(s)	1245, 1292, 1607, 1932
BfmI	CTRYAG	1 cut(s)	560
BfoI	RGCGCY	4 cut(s)	497, 614, 749, 785
BfuAI	ACCTGC	2 cut(s)	101, 1685
BglI	GCCNNNNNGGC	1 cut(s)	608
BglII	AGATCT	2 cut(s)	935, 1343
Bme1390I	CCNGG	8 cut(s)	16, 174, 533, 539, 851, 1326, 1363, 1692
Bme18I	GGWCC	3 cut(s)	535, 1047, 1147
BmgT120I	GGNCC	3 cut(s)	535, 1047, 1147
BmiI	GGNNCC	7 cut(s)	537, 557, 783, 1049, 1649, 1768, 1979
BmrFI	CCNGG	8 cut(s)	16, 174, 533, 539, 851, 1326, 1363, 1692
BmsI	GCATC	7 cut(s)	953, 985, 1051, 1417, 1447, 1880, 1894
BplI	GAGNNNNNCTC	2 cut(s)	134, 166
BpmI	CTGGAG	1 cut(s)	1517
Bpu10I	CCTNAGC	1 cut(s)	1461
BpuEI	CTTGAG	3 cut(s)	1595, 1616, 1726
BpuMI	CCSGG	7 cut(s)	16, 174, 533, 539, 851, 1363, 1692
Bsa29I	ATCGAT	1 cut(s)	516
BsaAI	YACGTR	1 cut(s)	1583
BsaHI	GRCGYC	3 cut(s)	5, 288, 782
BsaJI	CCNNGG	8 cut(s)	129, 172, 280, 603, 661, 735, 741, 813
BsaWI	WCCGGW	2 cut(s)	1252, 1877
BsaXI	ACNNNNNCTCC	4 cut(s)	335, 365, 1184, 1214
Bsc4I	CCNNNNNNNGG	7 cut(s)	53, 538, 1044, 1167, 1259, 1600, 1719
Bse118I	RCCGGY	4 cut(s)	412, 896, 998, 1877
Bse1I	ACTGG	4 cut(s)	621, 680, 722, 1515
Bse3DI	GCAATG	4 cut(s)	153, 251, 1429, 1933
BseAI	TCCGGA	1 cut(s)	1252
BseBI	CCWGG	1 cut(s)	1326
BseCI	ATCGAT	1 cut(s)	516
BseDI	CCNNGG	8 cut(s)	129, 172, 280, 603, 661, 735, 741, 813
BseGI	GGATG	2 cut(s)	1228, 1462
BseLI	CCNNNNNNNGG	7 cut(s)	53, 538, 1044, 1167, 1259, 1600, 1719
BseMI	GCAATG	4 cut(s)	153, 251, 1429, 1933
BseMII	CTCAG	5 cut(s)	494, 1419, 1452, 1483, 1568
BseNI	ACTGG	4 cut(s)	621, 680, 722, 1515
BseRI	GAGGAG	3 cut(s)	248, 960, 1192
BseSI	GKGCMC	1 cut(s)	1590
BseX3I	CGGCCG	1 cut(s)	738
BseXI	GCAGC	6 cut(s)	45, 493, 574, 720, 1667, 1674
BsgI	GTGCAG	1 cut(s)	1716
Bsh1236I	CGCG	8 cut(s)	84, 117, 342, 512, 653, 737, 1482, 1842
Bsh1285I	CGRYCG	3 cut(s)	115, 516, 741
BshFI	GGCC	5 cut(s)	134, 602, 734, 740, 1593
BshNI	GGYRCC	1 cut(s)	781
BshTI	ACCGGT	1 cut(s)	1877
BshVI	ATCGAT	1 cut(s)	516
BsiEI	CGRYCG	3 cut(s)	115, 516, 741
BsiHKAI	GWGCWC	3 cut(s)	147, 1553, 1590
BslFI	GGGAC	2 cut(s)	1019, 1033
BslI	CCNNNNNNNGG	7 cut(s)	53, 538, 1044, 1167, 1259, 1600, 1719
BsmAI	GTCTC	4 cut(s)	12, 341, 764, 860
BsmBI	CGTCTC	2 cut(s)	12, 341
BsmFI	GGGAC	2 cut(s)	1019, 1033
BsmI	GAATGC	3 cut(s)	201, 814, 1573
BsnI	GGCC	5 cut(s)	134, 602, 734, 740, 1593
Bsp1286I	GDGCHC	3 cut(s)	147, 1553, 1590
Bsp13I	TCCGGA	1 cut(s)	1252
Bsp1407I	TGTACA	1 cut(s)	1964
Bsp19I	CCATGG	1 cut(s)	129
BspANI	GGCC	5 cut(s)	134, 602, 734, 740, 1593
BspCNI	CTCAG	5 cut(s)	493, 1418, 1453, 1482, 1567
BspDI	ATCGAT	1 cut(s)	516
BspEI	TCCGGA	1 cut(s)	1252
BspFNI	CGCG	8 cut(s)	84, 117, 342, 512, 653, 737, 1482, 1842
BspHI	TCATGA	1 cut(s)	1390
BspLI	GGNNCC	7 cut(s)	537, 557, 783, 1049, 1649, 1768, 1979
BspMAI	CTGCAG	1 cut(s)	564
BspMI	ACCTGC	2 cut(s)	101, 1685
BspPI	GGATC	8 cut(s)	64, 78, 357, 1244, 1471, 1473, 1642, 1655
BspQI	GCTCTTC	1 cut(s)	578
BspT107I	GGYRCC	1 cut(s)	781
BsrDI	GCAATG	4 cut(s)	153, 251, 1429, 1933
BsrFI	RCCGGY	4 cut(s)	412, 896, 998, 1877
BsrGI	TGTACA	1 cut(s)	1964
BsrI	ACTGG	4 cut(s)	621, 680, 722, 1515
BssAI	RCCGGY	4 cut(s)	412, 896, 998, 1877
BssECI	CCNNGG	8 cut(s)	129, 172, 280, 603, 661, 735, 741, 813
BssNAI	GTATAC	1 cut(s)	1339
BssNI	GRCGYC	3 cut(s)	5, 288, 782
BssT1I	CCWWGG	4 cut(s)	129, 603, 661, 813
Bst1107I	GTATAC	1 cut(s)	1339
Bst2UI	CCWGG	1 cut(s)	1326
Bst4CI	ACNGT	3 cut(s)	325, 766, 1033
Bst6I	CTCTTC	2 cut(s)	223, 578
BstACI	GRCGYC	3 cut(s)	5, 288, 782
BstAPI	GCANNNNNTGC	2 cut(s)	246, 1664
BstAUI	TGTACA	1 cut(s)	1964
BstBAI	YACGTR	1 cut(s)	1583
BstDEI	CTNAG	6 cut(s)	480, 1405, 1461, 1469, 1554, 2001
BstDSI	CCRYGG	3 cut(s)	129, 280, 735
BstEII	GGTNACC	2 cut(s)	1072, 1210
BstF5I	GGATG	2 cut(s)	1228, 1462
BstFNI	CGCG	8 cut(s)	84, 117, 342, 512, 653, 737, 1482, 1842
BstH2I	RGCGCY	4 cut(s)	497, 614, 749, 785
BstHHI	GCGC	6 cut(s)	496, 526, 613, 653, 748, 784
BstMAI	GTCTC	4 cut(s)	12, 341, 764, 860
BstMCI	CGRYCG	3 cut(s)	115, 516, 741
BstNI	CCWGG	1 cut(s)	1326
BstNSI	RCATGY	1 cut(s)	1965
BstPI	GGTNACC	2 cut(s)	1072, 1210
BstSCI	CCNGG	8 cut(s)	14, 172, 531, 537, 849, 1324, 1361, 1690
BstSFI	CTRYAG	1 cut(s)	560
BstSLI	GKGCMC	1 cut(s)	1590
BstUI	CGCG	8 cut(s)	84, 117, 342, 512, 653, 737, 1482, 1842
BstV1I	GCAGC	6 cut(s)	45, 493, 574, 720, 1667, 1674
BstX2I	RGATCY	7 cut(s)	56, 70, 935, 1249, 1343, 1465, 1647
BstYI	RGATCY	7 cut(s)	56, 70, 935, 1249, 1343, 1465, 1647
BstZ17I	GTATAC	1 cut(s)	1339
BstZI	CGGCCG	1 cut(s)	738
Bsu15I	ATCGAT	1 cut(s)	516
BsuRI	GGCC	5 cut(s)	134, 602, 734, 740, 1593
BsuTUI	ATCGAT	1 cut(s)	516
BtgI	CCRYGG	3 cut(s)	129, 280, 735
BtsCI	GGATG	2 cut(s)	1228, 1462
BtsI	GCAGTG	1 cut(s)	1125
BtsIMutI	CAGTG	1 cut(s)	1125
BveI	ACCTGC	2 cut(s)	101, 1685
CaiI	CAGNNNCTG	2 cut(s)	1127, 1664
CciI	TCATGA	1 cut(s)	1390
CfoI	GCGC	6 cut(s)	496, 526, 613, 653, 748, 784
Cfr10I	RCCGGY	4 cut(s)	412, 896, 998, 1877
Cfr13I	GGNCC	3 cut(s)	535, 1047, 1147
Cfr42I	CCGCGG	1 cut(s)	738
ClaI	ATCGAT	1 cut(s)	516
CseI	GACGC	4 cut(s)	73, 296, 642, 786
Csp6I	GTAC	2 cut(s)	1830, 1965
CspAI	ACCGGT	1 cut(s)	1877
CviAII	CATG	4 cut(s)	130, 391, 1391, 1962
CviQI	GTAC	2 cut(s)	1830, 1965
DdeI	CTNAG	6 cut(s)	480, 1405, 1461, 1469, 1554, 2001
DinI	GGCGCC	1 cut(s)	783
DraI	TTTAAA	1 cut(s)	1356
DrdI	GACNNNNNNGTC	1 cut(s)	356
DseDI	GACNNNNNNGTC	1 cut(s)	356
EaeI	YGGCCR	1 cut(s)	738
EagI	CGGCCG	1 cut(s)	738
Eam1104I	CTCTTC	2 cut(s)	223, 578
EarI	CTCTTC	2 cut(s)	223, 578
EciI	GGCGGA	3 cut(s)	127, 320, 376
Ecl136II	GAGCTC	2 cut(s)	145, 1551
EclXI	CGGCCG	1 cut(s)	738
Eco130I	CCWWGG	4 cut(s)	129, 603, 661, 813
Eco24I	GRGCYC	2 cut(s)	147, 1553
Eco47I	GGWCC	3 cut(s)	535, 1047, 1147
Eco52I	CGGCCG	1 cut(s)	738
Eco53kI	GAGCTC	2 cut(s)	145, 1551
Eco57I	CTGAAG	2 cut(s)	95, 569
Eco72I	CACGTG	1 cut(s)	1583
Eco91I	GGTNACC	2 cut(s)	1072, 1210
EcoICRI	GAGCTC	2 cut(s)	145, 1551
EcoO109I	RGGNCCY	1 cut(s)	1047
EcoO65I	GGTNACC	2 cut(s)	1072, 1210
EcoRI	GAATTC	2 cut(s)	475, 1700
EcoRII	CCWGG	1 cut(s)	1324
EcoT14I	CCWWGG	4 cut(s)	129, 603, 661, 813
EcoT38I	GRGCYC	2 cut(s)	147, 1553
EgeI	GGCGCC	1 cut(s)	783
EheI	GGCGCC	1 cut(s)	783
ErhI	CCWWGG	4 cut(s)	129, 603, 661, 813
Esp3I	CGTCTC	2 cut(s)	12, 341
FaeI	CATG	4 cut(s)	133, 394, 1394, 1965
FaqI	GGGAC	2 cut(s)	1019, 1033
FatI	CATG	4 cut(s)	129, 390, 1390, 1961
FbaI	TGATCA	1 cut(s)	1387
FblI	GTMKAC	1 cut(s)	1338
FokI	GGATG	2 cut(s)	1235, 1469
FriOI	GRGCYC	2 cut(s)	147, 1553
FspBI	CTAG	4 cut(s)	1245, 1292, 1607, 1932
GlaI	GCGC	6 cut(s)	495, 525, 612, 652, 747, 783
GsuI	CTGGAG	1 cut(s)	1517
HaeII	RGCGCY	4 cut(s)	497, 614, 749, 785
HaeIII	GGCC	5 cut(s)	134, 602, 734, 740, 1593
HgaI	GACGC	4 cut(s)	73, 296, 642, 786
HhaI	GCGC	6 cut(s)	496, 526, 613, 653, 748, 784
Hin1I	GRCGYC	3 cut(s)	5, 288, 782
Hin1II	CATG	4 cut(s)	133, 394, 1394, 1965
Hin6I	GCGC	6 cut(s)	494, 524, 611, 651, 746, 782
HinP1I	GCGC	6 cut(s)	494, 524, 611, 651, 746, 782
HphI	GGTGA	6 cut(s)	82, 253, 850, 1084, 1222, 1340
Hpy166II	GTNNAC	3 cut(s)	1339, 1588, 1830
Hpy8I	GTNNAC	3 cut(s)	1339, 1588, 1830
Hpy99I	CGWCG	7 cut(s)	290, 329, 359, 427, 463, 658, 802
HpyAV	CCTTC	6 cut(s)	37, 199, 724, 748, 877, 1638
HpyCH4III	ACNGT	3 cut(s)	325, 766, 1033
HpyCH4IV	ACGT	1 cut(s)	1582
HpyCH4V	TGCA	9 cut(s)	201, 562, 1430, 1588, 1683, 1697, 1871, 1900, 1926
HpyF3I	CTNAG	6 cut(s)	480, 1405, 1461, 1469, 1554, 2001
HpySE526I	ACGT	1 cut(s)	1582
Hsp92I	GRCGYC	3 cut(s)	5, 288, 782
Hsp92II	CATG	4 cut(s)	133, 394, 1394, 1965
HspAI	GCGC	6 cut(s)	494, 524, 611, 651, 746, 782
KasI	GGCGCC	1 cut(s)	781
Kpn2I	TCCGGA	1 cut(s)	1252
KroI	GCCGGC	1 cut(s)	896
KroNI	GCCGGC	1 cut(s)	898
Ksp22I	TGATCA	1 cut(s)	1387
KspI	CCGCGG	1 cut(s)	738
LguI	GCTCTTC	1 cut(s)	578
LmnI	GCTCC	7 cut(s)	301, 463, 555, 630, 874, 925, 1548
Lsp1109I	GCAGC	6 cut(s)	45, 493, 574, 720, 1667, 1674
LweI	GCATC	7 cut(s)	953, 985, 1051, 1417, 1447, 1880, 1894
MaeI	CTAG	4 cut(s)	1245, 1292, 1607, 1932
MaeII	ACGT	1 cut(s)	1582
MaeIII	GTNAC	6 cut(s)	1072, 1210, 1238, 1365, 1578, 1880
MboII	GAAGA	7 cut(s)	51, 172, 210, 595, 881, 930, 1445
MflI	RGATCY	7 cut(s)	56, 70, 935, 1249, 1343, 1465, 1647
MhlI	GDGCHC	3 cut(s)	147, 1553, 1590
MluCI	AATT	5 cut(s)	475, 924, 1172, 1179, 1700
Mly113I	GGCGCC	1 cut(s)	782
MlyI	GAGTC	5 cut(s)	143, 209, 344, 1397, 1586
MmeI	TCCRAC	2 cut(s)	651, 803
MroI	TCCGGA	1 cut(s)	1252
MroNI	GCCGGC	1 cut(s)	896
MroXI	GAANNNNTTC	2 cut(s)	912, 1848
MseI	TTAA	6 cut(s)	434, 1100, 1355, 1383, 1523, 1782
MslI	CAYNNNNRTG	2 cut(s)	1130, 1221
MspA1I	CMGCKG	3 cut(s)	547, 565, 737
MspR9I	CCNGG	8 cut(s)	16, 174, 533, 539, 851, 1326, 1363, 1692
Mva1269I	GAATGC	3 cut(s)	201, 814, 1573
MvaI	CCWGG	1 cut(s)	1326
MvnI	CGCG	8 cut(s)	84, 117, 342, 512, 653, 737, 1482, 1842
NaeI	GCCGGC	1 cut(s)	898
NarI	GGCGCC	1 cut(s)	782
NciI	CCSGG	7 cut(s)	16, 174, 533, 539, 851, 1363, 1692
NcoI	CCATGG	1 cut(s)	129
NgoMIV	GCCGGC	1 cut(s)	896
NlaIII	CATG	4 cut(s)	133, 394, 1394, 1965
NlaIV	GGNNCC	7 cut(s)	537, 557, 783, 1049, 1649, 1768, 1979
NmeAIII	GCCGAG	4 cut(s)	166, 470, 766, 1933
NmuCI	GTSAC	6 cut(s)	1072, 1210, 1238, 1365, 1578, 1880
NspI	RCATGY	1 cut(s)	1965
OliI	CACNNNNGTG	1 cut(s)	1130
PagI	TCATGA	1 cut(s)	1390
PaqCI	CACCTGC	1 cut(s)	101
PciI	ACATGT	1 cut(s)	1961
PciSI	GCTCTTC	1 cut(s)	578
PcsI	WCGNNNNNNNCGW	2 cut(s)	114, 578
PctI	GAATGC	3 cut(s)	201, 814, 1573
PdiI	GCCGGC	1 cut(s)	898
PdmI	GAANNNNTTC	2 cut(s)	912, 1848
PfeI	GAWTC	7 cut(s)	518, 902, 1012, 1256, 1321, 1661, 1823
PflFI	GACNNNGTC	1 cut(s)	353
PflMI	CCANNNNNTGG	2 cut(s)	1167, 1719
PfoI	TCCNGGA	1 cut(s)	849
PinAI	ACCGGT	1 cut(s)	1877
Ple19I	CGATCG	1 cut(s)	516
PleI	GAGTC	5 cut(s)	143, 209, 344, 1397, 1585
PluTI	GGCGCC	1 cut(s)	785
PmaCI	CACGTG	1 cut(s)	1583
PmlI	CACGTG	1 cut(s)	1583
PpsI	GAGTC	5 cut(s)	143, 209, 344, 1397, 1585
Ppu21I	YACGTR	1 cut(s)	1583
PpuMI	RGGWCCY	1 cut(s)	1047
PscI	ACATGT	1 cut(s)	1961
PsiI	TTATAA	1 cut(s)	792
Psp124BI	GAGCTC	2 cut(s)	147, 1553
Psp5II	RGGWCCY	1 cut(s)	1047
Psp6I	CCWGG	1 cut(s)	1324
PspCI	CACGTG	1 cut(s)	1583
PspEI	GGTNACC	2 cut(s)	1072, 1210
PspGI	CCWGG	1 cut(s)	1324
PspN4I	GGNNCC	7 cut(s)	537, 557, 783, 1049, 1649, 1768, 1979
PspPI	GGNCC	3 cut(s)	535, 1047, 1147
PspPPI	RGGWCCY	1 cut(s)	1047
PstI	CTGCAG	1 cut(s)	564
PstNI	CAGNNNCTG	2 cut(s)	1127, 1664
PsuI	RGATCY	7 cut(s)	56, 70, 935, 1249, 1343, 1465, 1647
PsyI	GACNNNGTC	1 cut(s)	353
PvuI	CGATCG	1 cut(s)	516
PvuII	CAGCTG	1 cut(s)	547
RsaI	GTAC	2 cut(s)	1831, 1966
RsaNI	GTAC	2 cut(s)	1830, 1965
RseI	CAYNNNNRTG	2 cut(s)	1130, 1221
SacI	GAGCTC	2 cut(s)	147, 1553
SacII	CCGCGG	1 cut(s)	738
SapI	GCTCTTC	1 cut(s)	578
SaqAI	TTAA	6 cut(s)	434, 1100, 1355, 1383, 1523, 1782
Sau96I	GGNCC	3 cut(s)	535, 1047, 1147
SchI	GAGTC	5 cut(s)	143, 209, 344, 1397, 1586
ScrFI	CCNGG	8 cut(s)	16, 174, 533, 539, 851, 1326, 1363, 1692
SduI	GDGCHC	3 cut(s)	147, 1553, 1590
SfaNI	GCATC	7 cut(s)	953, 985, 1051, 1417, 1447, 1880, 1894
SfcI	CTRYAG	1 cut(s)	560
SfoI	GGCGCC	1 cut(s)	783
Sfr303I	CCGCGG	1 cut(s)	738
SgrAI	CRCCGGYG	1 cut(s)	412
SgrBI	CCGCGG	1 cut(s)	738
SinI	GGWCC	3 cut(s)	535, 1047, 1147
SmiMI	CAYNNNNRTG	2 cut(s)	1130, 1221
SmlI	CTYRAG	3 cut(s)	1574, 1595, 1705
SmoI	CTYRAG	3 cut(s)	1574, 1595, 1705
Sse9I	AATT	5 cut(s)	475, 924, 1172, 1179, 1700
SspDI	GGCGCC	1 cut(s)	781
SspMI	CTAG	4 cut(s)	1245, 1292, 1607, 1932
SstI	GAGCTC	2 cut(s)	147, 1553
StyD4I	CCNGG	8 cut(s)	14, 172, 531, 537, 849, 1324, 1361, 1690
StyI	CCWWGG	4 cut(s)	129, 603, 661, 813
TaaI	ACNGT	3 cut(s)	325, 766, 1033
TaiI	ACGT	1 cut(s)	1585
TaqI	TCGA	8 cut(s)	147, 272, 516, 572, 656, 961, 1139, 1751
TasI	AATT	5 cut(s)	475, 924, 1172, 1179, 1700
TatI	WGTACW	2 cut(s)	1829, 1964
TauI	GCSGC	6 cut(s)	232, 512, 568, 611, 737, 740
TfiI	GAWTC	7 cut(s)	518, 902, 1012, 1256, 1321, 1661, 1823
Tru1I	TTAA	6 cut(s)	434, 1100, 1355, 1383, 1523, 1782
Tru9I	TTAA	6 cut(s)	434, 1100, 1355, 1383, 1523, 1782
TscAI	CASTG	1 cut(s)	1132
TseFI	GTSAC	6 cut(s)	1072, 1210, 1238, 1365, 1578, 1880
TseI	GCWGC	6 cut(s)	33, 506, 562, 708, 1680, 1687
Tsp45I	GTSAC	6 cut(s)	1072, 1210, 1238, 1365, 1578, 1880
TspDTI	ATGAA	2 cut(s)	875, 1626
TspGWI	ACGGA	3 cut(s)	288, 343, 476
TspRI	CASTG	1 cut(s)	1132
Tth111I	GACNNNGTC	1 cut(s)	353
Van91I	CCANNNNNTGG	2 cut(s)	1167, 1719
VneI	GTGCAC	1 cut(s)	1586
VpaK11BI	GGWCC	3 cut(s)	535, 1047, 1147
XapI	RAATTY	3 cut(s)	475, 1172, 1700
XbaI	TCTAGA	1 cut(s)	1606
XceI	RCATGY	1 cut(s)	1965
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1541
XmiI	GTMKAC	1 cut(s)	1338
XmnI	GAANNNNTTC	2 cut(s)	912, 1848
XspI	CTAG	4 cut(s)	1245, 1292, 1607, 1932

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.