Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0015612

Physical Location & Seq

Genomic Coordinates Forward (+)

6255274 .. 6256918

Gene Structure 1645 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1089200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 933 bp

ATGTCAAAGACTCAAATGGGCTCCGTTAATGGCAGGTTTTCCAGTTTTTCAGGTCGATACCCAGAAATGTCTGGCTCTGAAGATTTCCAGAGACAATTACAGCAGCTCTTCCACCTTCACGATTCCGGCTTAGATCAAGCTTTCATTGATGCTCTCCCTGTGTTCCTTTACAAAGAGATTAAGGGTTTGAAAGAACCATTTGATTGTCCAGTTTGTCTCTGTGAGTTTTGTGAGAAGGATCAGCTGAGATTGCTCCCAGTTTGCAGTCATGCTTTTCACATTGATTGCATCGACACATGGTTGCTGTCTAATTCGACTTGCCCTCTTTGTAGAGGGACCCTCTACACGCCAGGGGTTGCTATCGAAAACCCGGTTTTCGATTTTGATGATCTGATCGAACAAGATGGTTCTTTGGGAAATGGAGGAAATGGGGTTGGTCAAAAGTCTGCAGAGAATGAGATTGTCGGTGAGAAGAAGTTGTTTTCTGTGAGACTTGGGAAATTTAGAAGCACAAATGAGGAGGCAGGTGGTTGTGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGAGAGAGTAGAGGGAGAAACCAGCAGCAGCAATTTGGATGCAAGGAGATGTTACTCCATGGGATCATACCAATATGTGGTTGCAGATTTGGAGTTGCAGGTGGCTTTGAGGCCCAAAAGGGCAGGTGATAGCGTGAGGCTTGTGAGAGGGAGAGGTGGACAAAATGGGAATTCTACTAATTACAGTGTTGGAGGTGGAGATGCTGAAGGGAAGAAAATTAACAGTGCAAGTAAAGGTGAAAGCTTTTCTGTATCCAAGATTTGGATGTGGCCCAAGAAGGGTAAATTTCCAAATTCTGCAGAAACCCATGTGGGTAATTCCTCTGTTACTGTGGGTTTGCCATGGACTGATAGGTCTCAGGGTACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

311

Amino Acids

33.39

Weight (kDa)

5.82

Isoelectric Point (pI)

39.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	67 - 111	1.5e-08	RING-H2 zinc finger domain
zf-RING_2	PF13639	68 - 111	3.2e-13	Ring finger domain
zf-RING_11	PF17123	68 - 97	1.1e-07	RING-like zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015612)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g34840
malus_domestica	MD13G1089200.v1.1 MD16G1089400.v1.1
prunus_persica	Prupe.1G256600_v2.0.a1
pyrus_communis	pycom16g07650
rosa_chinensis	RchiOBHm_Chr4g0444181
rosa_laevigata	RLG00000005849
rosa_multiflora	Rmu_sc0001001.1_g000021 Rmu_sc0017354.1_g000001
rosa_roxburghii	Rroxscaffold_5G00384770
rosa_rugosa	Rorug04G0350700
rosa_samantha	Rh4AG411500 Rh4BG422900 Rh4CG437200 Rh4DG418200
rosa_wichuraiana	Rw4G035350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	3 cut(s)	515, 652, 677
AasI	GACNNNNNNGTC	1 cut(s)	916
Acc36I	ACCTGC	4 cut(s)	24, 515, 652, 677
AccB7I	CCANNNNNTGG	2 cut(s)	640, 825
AclWI	GGATC	2 cut(s)	246, 634
AcsI	RAATTY	4 cut(s)	500, 733, 848, 856
AcuI	CTGAAG	2 cut(s)	99, 789
AfaI	GTAC	1 cut(s)	928
AfiI	CCNNNNNNNGG	3 cut(s)	640, 825, 842
AgsI	TTSAA	1 cut(s)	190
AjnI	CCWGG	1 cut(s)	349
AluBI	AGCT	4 cut(s)	106, 140, 244, 807
AluI	AGCT	4 cut(s)	106, 140, 244, 807
Alw26I	GTCTC	4 cut(s)	85, 221, 484, 924
AlwI	GGATC	2 cut(s)	246, 634
AoxI	GGCC	2 cut(s)	674, 833
ApeKI	GCWGC	3 cut(s)	103, 588, 591
ApoI	RAATTY	4 cut(s)	500, 733, 848, 856
AspS9I	GGNCC	3 cut(s)	336, 675, 834
AsuC2I	CCSGG	1 cut(s)	371
AsuHPI	GGTGA	3 cut(s)	479, 701, 812
AvaII	GGWCC	1 cut(s)	336
BanII	GRGCYC	1 cut(s)	23
BbvI	GCAGC	3 cut(s)	115, 600, 603
BccI	CCATC	1 cut(s)	398
BciT130I	CCWGG	1 cut(s)	351
BciVI	GTATCC	1 cut(s)	826
BcnI	CCSGG	1 cut(s)	371
BcoDI	GTCTC	4 cut(s)	85, 221, 484, 924
BfmI	CTRYAG	2 cut(s)	447, 861
BfuAI	ACCTGC	4 cut(s)	24, 515, 652, 677
BfuI	GTATCC	1 cut(s)	826
BisI	GCNGC	3 cut(s)	104, 589, 592
BlsI	GCNGC	3 cut(s)	105, 590, 593
Bme1390I	CCNGG	2 cut(s)	351, 371
Bme18I	GGWCC	1 cut(s)	336
BmgT120I	GGNCC	3 cut(s)	336, 675, 834
BmiI	GGNNCC	3 cut(s)	22, 337, 338
BmrFI	CCNGG	2 cut(s)	351, 371
BmrI	ACTGGG	1 cut(s)	251
BmsI	GCATC	4 cut(s)	139, 297, 592, 754
BmuI	ACTGGG	1 cut(s)	251
BplI	GAGNNNNNCTC	2 cut(s)	324, 356
BpuMI	CCSGG	1 cut(s)	371
BsaI	GGTCTC	1 cut(s)	924
BsaJI	CCNNGG	3 cut(s)	350, 621, 905
BsaXI	ACNNNNNCTCC	2 cut(s)	555, 585
Bsc4I	CCNNNNNNNGG	3 cut(s)	640, 825, 842
Bse1I	ACTGG	3 cut(s)	42, 209, 257
BseBI	CCWGG	1 cut(s)	351
BseDI	CCNNGG	3 cut(s)	350, 621, 905
BseGI	GGATG	2 cut(s)	607, 834
BseLI	CCNNNNNNNGG	3 cut(s)	640, 825, 842
BseMII	CTCAG	1 cut(s)	236
BseNI	ACTGG	3 cut(s)	42, 209, 257
BseXI	GCAGC	3 cut(s)	115, 600, 603
BshFI	GGCC	2 cut(s)	676, 835
BsiSI	CCGG	2 cut(s)	126, 371
BslFI	GGGAC	1 cut(s)	349
BslI	CCNNNNNNNGG	3 cut(s)	640, 825, 842
BsmAI	GTCTC	4 cut(s)	85, 221, 484, 924
BsmFI	GGGAC	1 cut(s)	349
BsnI	GGCC	2 cut(s)	676, 835
Bso31I	GGTCTC	1 cut(s)	924
Bsp1286I	GDGCHC	1 cut(s)	23
Bsp143I	GATC	5 cut(s)	133, 238, 388, 393, 626
Bsp19I	CCATGG	2 cut(s)	621, 905
BspANI	GGCC	2 cut(s)	676, 835
BspCNI	CTCAG	1 cut(s)	237
BspLI	GGNNCC	3 cut(s)	22, 337, 338
BspMAI	CTGCAG	2 cut(s)	451, 865
BspMI	ACCTGC	4 cut(s)	24, 515, 652, 677
BspPI	GGATC	2 cut(s)	246, 634
BspQI	GCTCTTC	1 cut(s)	113
BspTNI	GGTCTC	1 cut(s)	924
BsrI	ACTGG	3 cut(s)	42, 209, 257
BssECI	CCNNGG	3 cut(s)	350, 621, 905
BssMI	GATC	5 cut(s)	133, 238, 388, 393, 626
BssT1I	CCWWGG	2 cut(s)	621, 905
Bst2UI	CCWGG	1 cut(s)	351
Bst4CI	ACNGT	3 cut(s)	749, 788, 895
Bst6I	CTCTTC	1 cut(s)	113
BstDEI	CTNAG	3 cut(s)	130, 245, 921
BstDSI	CCRYGG	2 cut(s)	621, 905
BstF5I	GGATG	2 cut(s)	607, 834
BstKTI	GATC	5 cut(s)	136, 241, 391, 396, 629
BstMAI	GTCTC	4 cut(s)	85, 221, 484, 924
BstMBI	GATC	5 cut(s)	133, 238, 388, 393, 626
BstMWI	GCNNNNNNNGC	1 cut(s)	250
BstNI	CCWGG	1 cut(s)	351
BstSCI	CCNGG	2 cut(s)	349, 369
BstSFI	CTRYAG	2 cut(s)	447, 861
BstV1I	GCAGC	3 cut(s)	115, 600, 603
BsuI	GTATCC	1 cut(s)	826
BsuRI	GGCC	2 cut(s)	676, 835
BtgI	CCRYGG	2 cut(s)	621, 905
BtsCI	GGATG	2 cut(s)	607, 834
BtsIMutI	CAGTG	2 cut(s)	754, 793
BveI	ACCTGC	4 cut(s)	24, 515, 652, 677
Cfr13I	GGNCC	3 cut(s)	336, 675, 834
Csp6I	GTAC	1 cut(s)	927
CviAII	CATG	6 cut(s)	269, 297, 622, 872, 906, 930
CviQI	GTAC	1 cut(s)	927
DdeI	CTNAG	3 cut(s)	130, 245, 921
DpnI	GATC	5 cut(s)	135, 240, 390, 395, 628
DpnII	GATC	5 cut(s)	133, 238, 388, 393, 626
DrdI	GACNNNNNNGTC	1 cut(s)	916
DseDI	GACNNNNNNGTC	1 cut(s)	916
Eam1104I	CTCTTC	1 cut(s)	113
EarI	CTCTTC	1 cut(s)	113
Eco130I	CCWWGG	2 cut(s)	621, 905
Eco24I	GRGCYC	1 cut(s)	23
Eco31I	GGTCTC	1 cut(s)	924
Eco47I	GGWCC	1 cut(s)	336
Eco57I	CTGAAG	2 cut(s)	99, 789
EcoO109I	RGGNCCY	1 cut(s)	336
EcoRI	GAATTC	1 cut(s)	733
EcoRII	CCWGG	1 cut(s)	349
EcoT14I	CCWWGG	2 cut(s)	621, 905
EcoT38I	GRGCYC	1 cut(s)	23
ErhI	CCWWGG	2 cut(s)	621, 905
FaeI	CATG	6 cut(s)	272, 300, 625, 875, 909, 933
FaiI	YATR	8 cut(s)	270, 298, 623, 631, 639, 873, 907, 931
FaqI	GGGAC	1 cut(s)	349
FatI	CATG	6 cut(s)	268, 296, 621, 871, 905, 929
Fnu4HI	GCNGC	3 cut(s)	104, 589, 592
FokI	GGATG	2 cut(s)	614, 841
FriOI	GRGCYC	1 cut(s)	23
Fsp4HI	GCNGC	3 cut(s)	104, 589, 592
GluI	GCNGC	3 cut(s)	104, 589, 592
HaeIII	GGCC	2 cut(s)	676, 835
HapII	CCGG	2 cut(s)	126, 371
Hin1II	CATG	6 cut(s)	272, 300, 625, 875, 909, 933
HindIII	AAGCTT	2 cut(s)	138, 805
HinfI	GANTC	2 cut(s)	10, 122
HpaII	CCGG	2 cut(s)	126, 371
HphI	GGTGA	3 cut(s)	479, 701, 812
Hpy166II	GTNNAC	1 cut(s)	722
Hpy188I	TCNGA	2 cut(s)	79, 393
Hpy188III	TCNNGA	2 cut(s)	88, 119
Hpy8I	GTNNAC	1 cut(s)	722
HpyAV	CCTTC	4 cut(s)	125, 229, 764, 835
HpyCH4III	ACNGT	3 cut(s)	749, 788, 895
HpyCH4V	TGCA	8 cut(s)	264, 288, 449, 605, 647, 661, 791, 863
HpyF10VI	GCNNNNNNNGC	1 cut(s)	250
HpyF3I	CTNAG	3 cut(s)	130, 245, 921
Hsp92II	CATG	6 cut(s)	272, 300, 625, 875, 909, 933
KflI	GGGWCCC	1 cut(s)	336
Kzo9I	GATC	5 cut(s)	133, 238, 388, 393, 626
LguI	GCTCTTC	1 cut(s)	113
LmnI	GCTCC	2 cut(s)	26, 258
Lsp1109I	GCAGC	3 cut(s)	115, 600, 603
LweI	GCATC	4 cut(s)	139, 297, 592, 754
MaeIII	GTNAC	2 cut(s)	614, 889
MalI	GATC	5 cut(s)	135, 240, 390, 395, 628
MboI	GATC	5 cut(s)	133, 238, 388, 393, 626
MboII	GAAGA	4 cut(s)	92, 100, 484, 787
MhlI	GDGCHC	1 cut(s)	23
MlyI	GAGTC	1 cut(s)	4
MmeI	TCCRAC	1 cut(s)	733
MseI	TTAA	3 cut(s)	27, 180, 783
MspA1I	CMGCKG	1 cut(s)	244
MspI	CCGG	2 cut(s)	126, 371
MspR9I	CCNGG	2 cut(s)	351, 371
MvaI	CCWGG	1 cut(s)	351
MwoI	GCNNNNNNNGC	1 cut(s)	250
NciI	CCSGG	1 cut(s)	371
NcoI	CCATGG	2 cut(s)	621, 905
NdeII	GATC	5 cut(s)	133, 238, 388, 393, 626
NlaIII	CATG	6 cut(s)	272, 300, 625, 875, 909, 933
NlaIV	GGNNCC	3 cut(s)	22, 337, 338
PaqCI	CACCTGC	3 cut(s)	515, 652, 677
PciSI	GCTCTTC	1 cut(s)	113
PfeI	GAWTC	1 cut(s)	122
PflMI	CCANNNNNTGG	2 cut(s)	640, 825
PkrI	GCNGC	3 cut(s)	105, 590, 593
PleI	GAGTC	1 cut(s)	4
PpsI	GAGTC	1 cut(s)	4
PpuMI	RGGWCCY	1 cut(s)	336
Psp5II	RGGWCCY	1 cut(s)	336
Psp6I	CCWGG	1 cut(s)	349
PspGI	CCWGG	1 cut(s)	349
PspN4I	GGNNCC	3 cut(s)	22, 337, 338
PspPI	GGNCC	3 cut(s)	336, 675, 834
PspPPI	RGGWCCY	1 cut(s)	336
PstI	CTGCAG	2 cut(s)	451, 865
PvuII	CAGCTG	1 cut(s)	244
RsaI	GTAC	1 cut(s)	928
RsaNI	GTAC	1 cut(s)	927
SapI	GCTCTTC	1 cut(s)	113
SaqAI	TTAA	3 cut(s)	27, 180, 783
SatI	GCNGC	3 cut(s)	104, 589, 592
Sau3AI	GATC	5 cut(s)	133, 238, 388, 393, 626
Sau96I	GGNCC	3 cut(s)	336, 675, 834
SchI	GAGTC	1 cut(s)	4
ScrFI	CCNGG	2 cut(s)	351, 371
SduI	GDGCHC	1 cut(s)	23
SfaNI	GCATC	4 cut(s)	139, 297, 592, 754
SfcI	CTRYAG	2 cut(s)	447, 861
SinI	GGWCC	1 cut(s)	336
StyD4I	CCNGG	2 cut(s)	349, 369
StyI	CCWWGG	2 cut(s)	621, 905
TaaI	ACNGT	3 cut(s)	749, 788, 895
TaqI	TCGA	6 cut(s)	55, 291, 314, 363, 378, 396
TfiI	GAWTC	1 cut(s)	122
Tru1I	TTAA	3 cut(s)	27, 180, 783
Tru9I	TTAA	3 cut(s)	27, 180, 783
TscAI	CASTG	2 cut(s)	754, 793
TseI	GCWGC	3 cut(s)	103, 588, 591
TspDTI	ATGAA	1 cut(s)	133
TspGWI	ACGGA	1 cut(s)	13
TspRI	CASTG	2 cut(s)	754, 793
Van91I	CCANNNNNTGG	2 cut(s)	640, 825
VpaK11BI	GGWCC	1 cut(s)	336
XapI	RAATTY	4 cut(s)	500, 733, 848, 856

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.