Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0015612

Physical Location & Seq

Genomic Coordinates Forward (+)

6190468 .. 6191717

Gene Structure 1250 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1089400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1122 bp

ATGGTGAATTTAGTGTGCCACTACCCTGAAATGTCTTGGAATCAAGCTCAATTCAGGCAAAGAAGTTTGCTTTCCTCTTCACCGTCATCATCCTCTGGGGGTAAAATTGCCCCATCAGTTCTATTTATCATAATTGTTCTAGCTGTTATATTTTTTGTATCAGGAATCCTCCACCTGCTTGTTAGATTTCTCACAAAGCATAGATCTTCATCCATATCTGGATCCAATAGATACCCAGAAATGTCTGGCTCTGAAGTTTTCCAGAGACAATTGCAGCAGCTCTTCCATCTCCACGATTCCGGCTTGGATCAAGCTTTCATTGATGCTCTCCCTGTGTTCCTTTACAAAGAGATTAAGGGTCTGAAAGAACCATTTGATTGTCCAGTTTGTCTGTGTGAGTTTTGTGAGAAGGATCGGCTGAGATTGCTCCCAATTTGTAGTCATGCTTTTCACATTGATTGCATTGACACATGGTTGTTGTCTAATTCGACTTGCCCTCTTTGTAGAGGGACCCTCTACACTCCAGGTATTGCTATCGAAAACCCGGTTTTCGATTTTGGTGATCTGATGGAAGAAGGTGGTTCTTTTGGGAATGGAGGAAGTGGGGTTCTTGCCGGTCAAAAGTCTGCAGATAATGAGATTGCGGGTGAGATGGTGTTTTCTGTGAGACTTGGGAAATTTAGAAGCACAAATGAGGAGGGAGTTGGTGGTGGCGGCGGCGGAGGAGAGAGAGTAGAGGGAGAAACCAGCAGAAGCAATTTGGATGCAAGGAGATGTTACTCAATGGGATCGTACCAATATGTGGTGGCAGATTTGGAGCTGCAGGTGGCTTTGAGGCCAAAAAGGGCAGGTGAGAGTGTGAGGCTTGTGAGGGGGAGAGTGGGACAAAATGGGAATTCTAGTAATTACAGTGTTGGTGGTGGAGATGCTGAGGGGAAGAAAATTAACAGTGCAAGTAAAGGTGATAGCTTTTCTGTATCCAAGATTTGGATGTGGCCTAAGAAGGGTAAATTTCCAAGTTCTGCAGAAACCCATGTGGGCCATGTGGGTAATTCCTCTCTTACTGTGGGTTTGCCATGGAGTGATAGATCTCAGGAAATTTCCATGTCTTTCCTGCCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

374

Amino Acids

40.6

Weight (kDa)

7.09

Isoelectric Point (pI)

49.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	125 - 169	2.7e-08	RING-H2 zinc finger domain
zf-RING_2	PF13639	126 - 169	4.5e-13	Ring finger domain
zf-RING_11	PF17123	126 - 155	1.6e-07	RING-like zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015612)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g34840
malus_domestica	MD13G1089200.v1.1 MD16G1089400.v1.1
prunus_persica	Prupe.1G256600_v2.0.a1
pyrus_communis	pycom16g07650
rosa_chinensis	RchiOBHm_Chr4g0444181
rosa_laevigata	RLG00000005849
rosa_multiflora	Rmu_sc0001001.1_g000021 Rmu_sc0017354.1_g000001
rosa_roxburghii	Rroxscaffold_5G00384770
rosa_rugosa	Rorug04G0350700
rosa_samantha	Rh4AG411500 Rh4BG422900 Rh4CG437200 Rh4DG418200
rosa_wichuraiana	Rw4G035350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	3 cut(s)	183, 814, 839
Acc36I	ACCTGC	3 cut(s)	183, 814, 839
AccB7I	CCANNNNNTGG	2 cut(s)	802, 987
AciI	CCGC	4 cut(s)	644, 714, 717, 720
AclWI	GGATC	5 cut(s)	216, 229, 315, 420, 796
AcsI	RAATTY	5 cut(s)	7, 677, 895, 1010, 1098
AcuI	CTGAAG	1 cut(s)	273
AfaI	GTAC	1 cut(s)	794
AfiI	CCNNNNNNNGG	3 cut(s)	802, 987, 1004
AjnI	CCWGG	1 cut(s)	523
AloI	GAACNNNNNNTCC	2 cut(s)	591, 623
AluBI	AGCT	6 cut(s)	47, 143, 280, 314, 820, 969
AluI	AGCT	6 cut(s)	47, 143, 280, 314, 820, 969
Alw26I	GTCTC	2 cut(s)	259, 661
AlwI	GGATC	5 cut(s)	216, 229, 315, 420, 796
AoxI	GGCC	3 cut(s)	836, 995, 1039
ApeKI	GCWGC	3 cut(s)	274, 277, 820
ApoI	RAATTY	5 cut(s)	7, 677, 895, 1010, 1098
AspS9I	GGNCC	2 cut(s)	510, 1039
AsuC2I	CCSGG	1 cut(s)	545
AsuHPI	GGTGA	6 cut(s)	16, 72, 572, 659, 863, 974
AvaII	GGWCC	1 cut(s)	510
BamHI	GGATCC	1 cut(s)	221
BbvCI	CCTCAGC	1 cut(s)	930
BbvI	GCAGC	3 cut(s)	286, 289, 807
BccI	CCATC	4 cut(s)	121, 294, 562, 646
BceAI	ACGGC	1 cut(s)	1102
BciT130I	CCWGG	1 cut(s)	525
BciVI	GTATCC	1 cut(s)	988
BcnI	CCSGG	1 cut(s)	545
BcoDI	GTCTC	2 cut(s)	259, 661
BfaI	CTAG	2 cut(s)	140, 900
BfmI	CTRYAG	3 cut(s)	627, 821, 1023
BfuAI	ACCTGC	3 cut(s)	183, 814, 839
BfuI	GTATCC	1 cut(s)	988
BglII	AGATCT	2 cut(s)	203, 1088
BisI	GCNGC	5 cut(s)	275, 278, 715, 718, 821
BlsI	GCNGC	5 cut(s)	276, 279, 716, 719, 822
Bme1390I	CCNGG	2 cut(s)	525, 545
Bme18I	GGWCC	1 cut(s)	510
BmgT120I	GGNCC	2 cut(s)	510, 1039
BmiI	GGNNCC	3 cut(s)	223, 511, 512
BmrFI	CCNGG	2 cut(s)	525, 545
BmsI	GCATC	3 cut(s)	313, 754, 916
BplI	GAGNNNNNCTC	2 cut(s)	498, 530
BpmI	CTGGAG	1 cut(s)	507
Bpu10I	CCTNAGC	1 cut(s)	930
BpuMI	CCSGG	1 cut(s)	545
BsaBI	GATNNNNATC	1 cut(s)	208
BsaJI	CCNNGG	1 cut(s)	1076
BsaXI	ACNNNNNCTCC	2 cut(s)	717, 747
Bsc4I	CCNNNNNNNGG	3 cut(s)	802, 987, 1004
Bse118I	RCCGGY	1 cut(s)	614
Bse1I	ACTGG	1 cut(s)	383
Bse8I	GATNNNNATC	1 cut(s)	208
BseBI	CCWGG	1 cut(s)	525
BseDI	CCNNGG	1 cut(s)	1076
BseGI	GGATG	4 cut(s)	89, 209, 769, 996
BseJI	GATNNNNATC	1 cut(s)	208
BseLI	CCNNNNNNNGG	3 cut(s)	802, 987, 1004
BseMII	CTCAG	3 cut(s)	410, 921, 1106
BseNI	ACTGG	1 cut(s)	383
BseRI	GAGGAG	2 cut(s)	710, 738
BseXI	GCAGC	3 cut(s)	286, 289, 807
BshFI	GGCC	3 cut(s)	838, 997, 1041
BsiSI	CCGG	3 cut(s)	300, 545, 615
BslFI	GGGAC	2 cut(s)	523, 897
BslI	CCNNNNNNNGG	3 cut(s)	802, 987, 1004
BsmAI	GTCTC	2 cut(s)	259, 661
BsmFI	GGGAC	2 cut(s)	523, 897
BsnI	GGCC	3 cut(s)	838, 997, 1041
Bsp143I	GATC	7 cut(s)	203, 221, 307, 412, 562, 788, 1088
Bsp19I	CCATGG	1 cut(s)	1076
BspACI	CCGC	4 cut(s)	644, 714, 717, 720
BspANI	GGCC	3 cut(s)	838, 997, 1041
BspCNI	CTCAG	3 cut(s)	411, 922, 1105
BspLI	GGNNCC	3 cut(s)	223, 511, 512
BspMAI	CTGCAG	3 cut(s)	631, 825, 1027
BspMI	ACCTGC	3 cut(s)	183, 814, 839
BspPI	GGATC	5 cut(s)	216, 229, 315, 420, 796
BspQI	GCTCTTC	1 cut(s)	287
BsrFI	RCCGGY	1 cut(s)	614
BsrI	ACTGG	1 cut(s)	383
BssAI	RCCGGY	1 cut(s)	614
BssECI	CCNNGG	1 cut(s)	1076
BssMI	GATC	7 cut(s)	203, 221, 307, 412, 562, 788, 1088
BssT1I	CCWWGG	1 cut(s)	1076
Bst2UI	CCWGG	1 cut(s)	525
Bst4CI	ACNGT	4 cut(s)	84, 911, 950, 1066
Bst6I	CTCTTC	2 cut(s)	82, 287
BstDEI	CTNAG	4 cut(s)	419, 930, 999, 1092
BstDSI	CCRYGG	1 cut(s)	1076
BstF5I	GGATG	4 cut(s)	89, 209, 769, 996
BstKTI	GATC	7 cut(s)	206, 224, 310, 415, 565, 791, 1091
BstMAI	GTCTC	2 cut(s)	259, 661
BstMBI	GATC	7 cut(s)	203, 221, 307, 412, 562, 788, 1088
BstMWI	GCNNNNNNNGC	1 cut(s)	424
BstNI	CCWGG	1 cut(s)	525
BstSCI	CCNGG	2 cut(s)	523, 543
BstSFI	CTRYAG	3 cut(s)	627, 821, 1023
BstV1I	GCAGC	3 cut(s)	286, 289, 807
BstX2I	RGATCY	3 cut(s)	203, 221, 1088
BstYI	RGATCY	3 cut(s)	203, 221, 1088
BsuI	GTATCC	1 cut(s)	988
BsuRI	GGCC	3 cut(s)	838, 997, 1041
BtgI	CCRYGG	1 cut(s)	1076
BtsCI	GGATG	4 cut(s)	89, 209, 769, 996
BtsIMutI	CAGTG	2 cut(s)	916, 955
BveI	ACCTGC	3 cut(s)	183, 814, 839
Cfr10I	RCCGGY	1 cut(s)	614
Cfr13I	GGNCC	2 cut(s)	510, 1039
Csp6I	GTAC	1 cut(s)	793
CviAII	CATG	6 cut(s)	443, 471, 1034, 1043, 1077, 1105
CviQI	GTAC	1 cut(s)	793
DdeI	CTNAG	4 cut(s)	419, 930, 999, 1092
DpnI	GATC	7 cut(s)	205, 223, 309, 414, 564, 790, 1090
DpnII	GATC	7 cut(s)	203, 221, 307, 412, 562, 788, 1088
Eam1104I	CTCTTC	2 cut(s)	82, 287
EarI	CTCTTC	2 cut(s)	82, 287
EciI	GGCGGA	1 cut(s)	735
Eco130I	CCWWGG	1 cut(s)	1076
Eco47I	GGWCC	1 cut(s)	510
Eco57I	CTGAAG	1 cut(s)	273
EcoO109I	RGGNCCY	1 cut(s)	510
EcoRI	GAATTC	1 cut(s)	895
EcoRII	CCWGG	1 cut(s)	523
EcoT14I	CCWWGG	1 cut(s)	1076
ErhI	CCWWGG	1 cut(s)	1076
FaeI	CATG	6 cut(s)	446, 474, 1037, 1046, 1080, 1108
FaqI	GGGAC	2 cut(s)	523, 897
FatI	CATG	6 cut(s)	442, 470, 1033, 1042, 1076, 1104
FauI	CCCGC	1 cut(s)	637
Fnu4HI	GCNGC	5 cut(s)	275, 278, 715, 718, 821
FokI	GGATG	4 cut(s)	76, 196, 776, 1003
Fsp4HI	GCNGC	5 cut(s)	275, 278, 715, 718, 821
FspBI	CTAG	2 cut(s)	140, 900
GluI	GCNGC	5 cut(s)	275, 278, 715, 718, 821
GsuI	CTGGAG	1 cut(s)	507
HaeIII	GGCC	3 cut(s)	838, 997, 1041
HapII	CCGG	3 cut(s)	300, 545, 615
Hin1II	CATG	6 cut(s)	446, 474, 1037, 1046, 1080, 1108
HindIII	AAGCTT	1 cut(s)	312
HinfI	GANTC	3 cut(s)	40, 165, 296
HpaII	CCGG	3 cut(s)	300, 545, 615
HphI	GGTGA	6 cut(s)	16, 72, 572, 659, 863, 974
Hpy188I	TCNGA	3 cut(s)	253, 363, 567
Hpy188III	TCNNGA	4 cut(s)	162, 219, 262, 1094
HpyAV	CCTTC	3 cut(s)	403, 569, 997
HpyCH4III	ACNGT	4 cut(s)	84, 911, 950, 1066
HpyCH4V	TGCA	7 cut(s)	274, 462, 629, 767, 823, 953, 1025
HpyF10VI	GCNNNNNNNGC	1 cut(s)	424
HpyF3I	CTNAG	4 cut(s)	419, 930, 999, 1092
Hsp92II	CATG	6 cut(s)	446, 474, 1037, 1046, 1080, 1108
KflI	GGGWCCC	1 cut(s)	510
Kzo9I	GATC	7 cut(s)	203, 221, 307, 412, 562, 788, 1088
LguI	GCTCTTC	1 cut(s)	287
LmnI	GCTCC	2 cut(s)	432, 817
Lsp1109I	GCAGC	3 cut(s)	286, 289, 807
LweI	GCATC	3 cut(s)	313, 754, 916
MaeI	CTAG	2 cut(s)	140, 900
MaeIII	GTNAC	1 cut(s)	776
MalI	GATC	7 cut(s)	205, 223, 309, 414, 564, 790, 1090
MboI	GATC	7 cut(s)	203, 221, 307, 412, 562, 788, 1088
MboII	GAAGA	5 cut(s)	69, 198, 274, 584, 949
MfeI	CAATTG	1 cut(s)	269
MflI	RGATCY	3 cut(s)	203, 221, 1088
MseI	TTAA	2 cut(s)	354, 945
MspI	CCGG	3 cut(s)	300, 545, 615
MspR9I	CCNGG	2 cut(s)	525, 545
MunI	CAATTG	1 cut(s)	269
MvaI	CCWGG	1 cut(s)	525
MwoI	GCNNNNNNNGC	1 cut(s)	424
NciI	CCSGG	1 cut(s)	545
NcoI	CCATGG	1 cut(s)	1076
NdeII	GATC	7 cut(s)	203, 221, 307, 412, 562, 788, 1088
NlaIII	CATG	6 cut(s)	446, 474, 1037, 1046, 1080, 1108
NlaIV	GGNNCC	3 cut(s)	223, 511, 512
PaqCI	CACCTGC	3 cut(s)	183, 814, 839
PciSI	GCTCTTC	1 cut(s)	287
PfeI	GAWTC	3 cut(s)	40, 165, 296
PflMI	CCANNNNNTGG	2 cut(s)	802, 987
PkrI	GCNGC	5 cut(s)	276, 279, 716, 719, 822
PpuMI	RGGWCCY	1 cut(s)	510
Psp5II	RGGWCCY	1 cut(s)	510
Psp6I	CCWGG	1 cut(s)	523
PspGI	CCWGG	1 cut(s)	523
PspN4I	GGNNCC	3 cut(s)	223, 511, 512
PspPI	GGNCC	2 cut(s)	510, 1039
PspPPI	RGGWCCY	1 cut(s)	510
PstI	CTGCAG	3 cut(s)	631, 825, 1027
PsuI	RGATCY	3 cut(s)	203, 221, 1088
RsaI	GTAC	1 cut(s)	794
RsaNI	GTAC	1 cut(s)	793
SapI	GCTCTTC	1 cut(s)	287
SaqAI	TTAA	2 cut(s)	354, 945
SatI	GCNGC	5 cut(s)	275, 278, 715, 718, 821
Sau3AI	GATC	7 cut(s)	203, 221, 307, 412, 562, 788, 1088
Sau96I	GGNCC	2 cut(s)	510, 1039
ScrFI	CCNGG	2 cut(s)	525, 545
SfaNI	GCATC	3 cut(s)	313, 754, 916
SfcI	CTRYAG	3 cut(s)	627, 821, 1023
SinI	GGWCC	1 cut(s)	510
SsiI	CCGC	4 cut(s)	644, 714, 717, 720
SspMI	CTAG	2 cut(s)	140, 900
StyD4I	CCNGG	2 cut(s)	523, 543
StyI	CCWWGG	1 cut(s)	1076
TaaI	ACNGT	4 cut(s)	84, 911, 950, 1066
TaqI	TCGA	3 cut(s)	488, 537, 552
TauI	GCSGC	2 cut(s)	717, 720
TfiI	GAWTC	3 cut(s)	40, 165, 296
Tru1I	TTAA	2 cut(s)	354, 945
Tru9I	TTAA	2 cut(s)	354, 945
TscAI	CASTG	2 cut(s)	916, 955
TseI	GCWGC	3 cut(s)	274, 277, 820
TspDTI	ATGAA	2 cut(s)	198, 307
TspRI	CASTG	2 cut(s)	916, 955
Van91I	CCANNNNNTGG	2 cut(s)	802, 987
VpaK11BI	GGWCC	1 cut(s)	510
XapI	RAATTY	5 cut(s)	7, 677, 895, 1010, 1098
XspI	CTAG	2 cut(s)	140, 900

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.