Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0008558

Physical Location & Seq

Genomic Coordinates Reverse (-)

11568673 .. 11581184

Gene Structure 12512 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1148100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3105 bp

ATGTTCTTCTCGGGCGATTCATCGACTCGGAAGCGAGTTGATTTGGGGGGACGGAGTACGAAGGAAAGGGACAGGCAGAAGCTTTTGGAGCAGACAAGGTTGGAACGCAACCGCCGGTTGTGGATACGACAGCAGAACTCGGCGGCTTTGAAAATCCAGAAATGCTTCAGAGGGAGGAAGGTGGCAGCGGCTGAACATTCTAAGGTGCGCGAGCAGTTCTACGGAAGATATGGGGGGCACTGCCAGAATGTCGACAGGTTTAGTTTTGGTCCCGATTCAGAGTTTCTCCGGCAGTTACTTTTCTTCTTTGATGCACGAAGCGTTGGTGATTTTTCCATCCTTGTGGAGACATGCCGGTTGCTTCATCAATTTGTCAGGGATACAGGGGATATTGTTAGCCTCATTGCGGGCATGGATTATTCGTCCAAGCATGCTTTGGTGAAACACAGAGTGAAGCAGCTTGCATATATCTGTATTAAGGCTGTTCATCAAAACAGAAATCAGTTGGAGGATCAACTATTTGCAGCCCCTGAGGAGCCCACCATGTCAACAACTTTATTGTTAGAAGCATTAGTTTTATTGATAGATCCCAAGCTTCCATGGGCTTGTGAGACAGTTGGCTATCTTTTGCAAAGAAAGGCTTTTGTCCTGTATAGAGAGATTATTTTTACAGGAAAGGAAAGTATAAGGACTCATAACTCAATTGGCAGAGTGTCTTCGTTGGAGCGCAGTCTTGCTGTTGTAATTTCTCACATTGGTCAGGAGCCATGTACGTGCCCAAACATCGATCCACATTGGAGTTTTTCATCTCAAATTCTTACAGTTCCTTTTTTATGGAAACTATTCCCCTACTTAGGAGAGGTTTTTGCAAGACAAGGGCTGAGCCAGCATTACATTAATCAAATGGCACTCTGTGTGAAAAGTCATGCTGATGTACTTCCGAAAGATGCATCAATTGAGTTACCCAGTTATGCCTGCCTTATTGGAAATATATTGGAATCTTCCGGAGTCGCTCTATCTCAGACTGACTGCTCATTTCAAATGGCTTTAGCCCTTGCTGGCGTTGCAACATTCTTGTTGGAGGCCCTTCCTTCAATGAAATCATCAAATAGAGAAAGCAAAGAAGATTCGATGGGTGATGATGACATGATTGAAGGAGATGATGCCATGGAAGTTTGTTTAAATAACGATTTAGAGCGACAGATATGTGATGCCATAGATCCGCGCTTTCTTCTGCAATTAACAAATGTTTTGTTTGGAGGTATTTCACTTGCCAGTGGCTCGCACCACGGACCTGATGATAAAGAGGTGTCAGCTGTTGGTGCGGCTTGTGCTTTTCTACATGTTACTTTCAACACCTTACCTCTGGAGAGAATCATGACTATACTTGCTTATAGAACGGAACTTGTTCCGGTGCTTTGGAATTTTATGAGACGGTGCCATGAAAATCAGAAATGGCAGTCAGTGTCTGAGCAGTTGGTGTATCTGCTGCCTGGAGATGCACCTGGCTGGCTATTACCTTTGGCTGTATTCTGTCCTGTGTACAAGCACATGCTTACAATAGTTGATAATGAAGAGTTCTATGAGCAGGAGAAGCCACTGTCATTGAAGGATATCAGATGCCTAGTTATCATACTCAGACAGGCCCTATGGCAACTTCTTTGGGTGAATCCTACAGCTCTCACTACTTCTATGAAACCTGTCACAACCTGCGCTTCTAATAAGAAGCACCCTGTGGAGTTAATTCAACATAGGGTTAGCATTGTAGCTTCTGAACTCCTCTCCCAGTTGCAAGATTGGAACAATAGACGTGAATTCACATCCCCCAGTGAGTTTCATGCTGATGGTGTCAATGAGTTCTTTATTGCACAGGCTGTGATAGAAAATACCCGGGCAAATGACATCATGAAGCAAGCTCCCTTCCTGGTACCATTCACAAGCAGAGTTAAAATATTCACTTCACAATTGGCAGCAGCTAGACAAAGACACGAGTCTAATTCTGTATTTACCAGAAACCGGTTCAGAATACGGCGGGATCGTATCTTGGAAGATGCTTATGATCAAATGAGTGCGCTGTCTGAAGATGATCTTCGGGGACCGATTCGTGTAACGTTTGTAAATGAATTTGGGGTTGAGGAGGCTGGAATTGATGGTGGCGGGATTTTCAAAGACTTCATGGAGAACATTACTCGAGCAGCCTTTGATGTTCAGTATGGATTGTTTAAGGAAACGTCCGATCATTTACTCTACCCTAATCCTGGTTCTGGAATGATTCACGAACAGCATCTCCAATTCTTTCACTTTCTTGGGGTTCTTCTTGCAAAGGCCATGTTTGAGGGGATTCTTGTAGATATACCATTTGCAACATTTTTTCTGAGCAAATTAAAACAAAAGTACAACTATTTAAATGATTTGCCTTCATTGGACCCAGAGTTGTACCGTCACCTTATTTTCTTGAAGCATTATAAGGGTGATATTTCAGAATTAGAATTGTACTTTGTCATTGTAAATAATGAATATGGAGAGCAAACAGAAGAGGAGCTGCTTCCCAGGGGAAAAAACCTACGTGTCACTAATGAGAACGTCATAACCTTTATTCATCTTGTTGCCAACCATCGTTTGAACTTTCAGATACGTCAACAAAGTTCACATTTTTTGAGGGGGTTTCAGCAGCTTATACCGAAAGATTGGATAGATATGTTTAATGAGCATGAACTTCAGCTTCTGATATCAGGTTCACTTGACAGCTTGGATGTTGATGATCTACGGATGAACACAAATTATGTGGGTGGCTATCATAGTGAGCATTATGTCATTGACATGTTCTGGGAAGGTCTGAAAAGTTTTTCATTGGAAAACCAGAAGAAATTTCTAAAATTTGTGACCGGTTGCTCTCGGGGACCTCTGCTTGGATTCAAATACTTGGAACCATTATTTTGCATACAGAGGGCTGGTGGCAATGCAGCTGAAGGAGCTCTTGACCGATTACCAACTGCAGCCACTTGTATGAATCTCCTAAAGCTTCCACCATATAGAAGTAAGGAGCAACTGGAATCAAAATTGATGTATGCTATAAGTGCAGATGCCGGCTTTGATTTGAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

HECT

Protein Analysis

1035

Amino Acids

117.96

Weight (kDa)

6.17

Isoelectric Point (pI)

43.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HECT	PF00632	729 - 1034	1.8e-92	HECT-domain (ubiquitin-transferase)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008558)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G17205 AT3G17205 AT3G17205
fragaria_vesca	FvH4_4g20760 FvH4_4g20760 FvH4_4g20760 FvH4_4g20760
malus_domestica	MD13G1148100.v1.1 MD16G1148200.v1.1
prunus_persica	Prupe.1G111600_v2.0.a1 Prupe.1G111600_v2.0.a1
pyrus_communis	pycom13g12750 pycom16g12650
rosa_chinensis	RchiOBHm_Chr4g0426201
rosa_laevigata	RLG00000007311
rosa_multiflora	Rmu_sc0000730.1_g000025
rosa_roxburghii	Rroxscaffold_5G00368220
rosa_rugosa	Rorug04G0209500
rosa_samantha	Rh4AG266300 Rh4BG272400 Rh4CG285600 Rh4DG268700
rosa_wichuraiana	Rw4G023030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2466
Acc36I	ACCTGC	1 cut(s)	1718
Acc65I	GGTACC	1 cut(s)	1927
AccB1I	GGYRCC	2 cut(s)	1437, 1927
AccI	GTMKAC	1 cut(s)	252
AccII	CGCG	2 cut(s)	210, 1225
AccIII	TCCGGA	1 cut(s)	1004
AciI	CCGC	8 cut(s)	112, 143, 188, 407, 1223, 1325, 2032, 2157
AclI	AACGTT	1 cut(s)	2111
AclWI	GGATC	5 cut(s)	519, 581, 782, 1214, 2043
AcsI	RAATTY	6 cut(s)	813, 1423, 1814, 2123, 2867, 2876
AcuI	CTGAAG	4 cut(s)	151, 2100, 2702, 2988
AdeI	CACNNNGTG	3 cut(s)	451, 914, 1735
AfaI	GTAC	8 cut(s)	58, 772, 936, 1544, 1929, 2396, 2438, 2495
AfiI	CCNNNNNNNGG	6 cut(s)	406, 854, 2016, 2258, 2264, 2909
AflIII	ACRYGT	3 cut(s)	1342, 2566, 2820
AgeI	ACCGGT	2 cut(s)	2016, 2885
AjiI	CACGTC	1 cut(s)	1811
AjnI	CCWGG	5 cut(s)	1492, 1504, 1923, 2257, 2549
AloI	GAACNNNNNNTCC	2 cut(s)	2271, 2303
Alw21I	GWGCWC	1 cut(s)	2977
Alw26I	GTCTC	3 cut(s)	341, 605, 1426
AlwI	GGATC	5 cut(s)	519, 581, 782, 1214, 2043
AlwNI	CAGNNNCTG	4 cut(s)	191, 530, 1469, 2725
Ama87I	CYCGRG	4 cut(s)	10, 1890, 2190, 2895
Aor13HI	TCCGGA	1 cut(s)	1004
AoxI	GGCC	3 cut(s)	1083, 1644, 2325
ApoI	RAATTY	6 cut(s)	813, 1423, 1814, 2123, 2867, 2876
AseI	ATTAAT	1 cut(s)	897
AsiGI	ACCGGT	2 cut(s)	2016, 2885
Asp700I	GAANNNNTTC	3 cut(s)	164, 842, 1407
Asp718I	GGTACC	1 cut(s)	1927
AspLEI	GCGC	5 cut(s)	210, 729, 1227, 1715, 2074
AspS9I	GGNCC	7 cut(s)	269, 1084, 1292, 1645, 2096, 2425, 2900
AsuC2I	CCSGG	2 cut(s)	1891, 1892
AsuHPI	GGTGA	6 cut(s)	338, 451, 1148, 1678, 2435, 2483
AvaI	CYCGRG	4 cut(s)	10, 1890, 2190, 2895
AvaII	GGWCC	5 cut(s)	269, 1292, 2096, 2425, 2900
AxyI	CCTNAGG	1 cut(s)	531
BaeGI	GKGCMC	2 cut(s)	240, 779
BanI	GGYRCC	2 cut(s)	1437, 1927
BanII	GRGCYC	2 cut(s)	540, 2977
BauI	CACGAG	1 cut(s)	1988
BbsI	GAAGAC	1 cut(s)	708
Bbv12I	GWGCWC	1 cut(s)	2977
BccI	CCATC	5 cut(s)	344, 1126, 1838, 2144, 2622
BceAI	ACGGC	1 cut(s)	2045
BciT130I	CCWGG	5 cut(s)	1494, 1506, 1925, 2259, 2551
BciVI	GTATCC	2 cut(s)	117, 373
BclI	TGATCA	1 cut(s)	2059
BcnI	CCSGG	2 cut(s)	1891, 1892
BcoDI	GTCTC	3 cut(s)	341, 605, 1426
BfaI	CTAG	2 cut(s)	1625, 1977
BfmI	CTRYAG	2 cut(s)	1674, 2994
BfuAI	ACCTGC	1 cut(s)	1718
BfuI	GTATCC	2 cut(s)	117, 373
BlpI	GCTNAGC	1 cut(s)	881
Bme1390I	CCNGG	7 cut(s)	1494, 1506, 1891, 1892, 1925, 2259, 2551
Bme18I	GGWCC	5 cut(s)	269, 1292, 2096, 2425, 2900
BmeT110I	CYCGRG	4 cut(s)	10, 1890, 2190, 2895
BmgBI	CACGTC	1 cut(s)	1811
BmgT120I	GGNCC	7 cut(s)	269, 1084, 1292, 1645, 2096, 2425, 2900
BmiI	GGNNCC	9 cut(s)	271, 537, 765, 1439, 1929, 2097, 2427, 2901, 2928
BmrFI	CCNGG	7 cut(s)	1494, 1506, 1891, 1892, 1925, 2259, 2551
BmrI	ACTGGG	3 cut(s)	960, 1780, 1821
BmuI	ACTGGG	3 cut(s)	960, 1780, 1821
BoxI	GACNNNNGTC	1 cut(s)	1990
BpiI	GAAGAC	1 cut(s)	708
BpmI	CTGGAG	2 cut(s)	1388, 1515
Bpu1102I	GCTNAGC	1 cut(s)	881
BpuMI	CCSGG	2 cut(s)	1891, 1892
Bsa29I	ATCGAT	1 cut(s)	786
BsaAI	YACGTR	2 cut(s)	774, 2567
BsaJI	CCNNGG	6 cut(s)	599, 1167, 1288, 1890, 2549, 2550
BsaWI	WCCGGW	4 cut(s)	1004, 1411, 2016, 2885
BsaXI	ACNNNNNCTCC	2 cut(s)	2271, 2301
Bsc4I	CCNNNNNNNGG	6 cut(s)	406, 854, 2016, 2258, 2264, 2909
Bse118I	RCCGGY	5 cut(s)	114, 354, 2016, 2885, 3086
Bse1I	ACTGG	5 cut(s)	966, 1275, 1786, 1827, 3054
Bse21I	CCTNAGG	1 cut(s)	531
Bse3DI	GCAATG	2 cut(s)	402, 2965
BseAI	TCCGGA	1 cut(s)	1004
BseBI	CCWGG	5 cut(s)	1494, 1506, 1925, 2259, 2551
BseCI	ATCGAT	1 cut(s)	786
BseDI	CCNNGG	6 cut(s)	599, 1167, 1288, 1890, 2549, 2550
BseGI	GGATG	4 cut(s)	336, 1820, 2758, 2775
BseLI	CCNNNNNNNGG	6 cut(s)	406, 854, 2016, 2258, 2264, 2909
BseMI	GCAATG	2 cut(s)	402, 2965
BseMII	CTCAG	6 cut(s)	522, 872, 1034, 1461, 1651, 2366
BseNI	ACTGG	5 cut(s)	966, 1275, 1786, 1827, 3054
BseRI	GAGGAG	4 cut(s)	548, 1769, 2150, 2552
BseSI	GKGCMC	2 cut(s)	240, 779
BsgI	GTGCAG	1 cut(s)	3099
Bsh1236I	CGCG	2 cut(s)	210, 1225
BshFI	GGCC	3 cut(s)	1085, 1646, 2327
BshNI	GGYRCC	2 cut(s)	1437, 1927
BshTI	ACCGGT	2 cut(s)	2016, 2885
BshVI	ATCGAT	1 cut(s)	786
BsiHKAI	GWGCWC	1 cut(s)	2977
BsiHKCI	CYCGRG	4 cut(s)	10, 1890, 2190, 2895
BsiSI	CCGG	9 cut(s)	115, 289, 355, 1005, 1412, 1891, 2017, 2886, 3087
BslFI	GGGAC	5 cut(s)	63, 83, 255, 2109, 2913
BslI	CCNNNNNNNGG	6 cut(s)	406, 854, 2016, 2258, 2264, 2909
BsmAI	GTCTC	3 cut(s)	341, 605, 1426
BsmBI	CGTCTC	1 cut(s)	1426
BsmFI	GGGAC	5 cut(s)	63, 83, 255, 2109, 2913
BsnI	GGCC	3 cut(s)	1085, 1646, 2327
BsoBI	CYCGRG	4 cut(s)	10, 1890, 2190, 2895
Bsp1286I	GDGCHC	4 cut(s)	240, 540, 779, 2977
Bsp13I	TCCGGA	1 cut(s)	1004
Bsp1407I	TGTACA	1 cut(s)	1542
Bsp143I	GATC	9 cut(s)	511, 586, 787, 1219, 2035, 2059, 2086, 2236, 2761
Bsp1720I	GCTNAGC	1 cut(s)	881
Bsp19I	CCATGG	2 cut(s)	599, 1167
BspACI	CCGC	8 cut(s)	112, 143, 188, 407, 1223, 1325, 2032, 2157
BspANI	GGCC	3 cut(s)	1085, 1646, 2327
BspCNI	CTCAG	6 cut(s)	523, 873, 1033, 1462, 1650, 2367
BspDI	ATCGAT	1 cut(s)	786
BspEI	TCCGGA	1 cut(s)	1004
BspFNI	CGCG	2 cut(s)	210, 1225
BspHI	TCATGA	2 cut(s)	1377, 1905
BspLI	GGNNCC	9 cut(s)	271, 537, 765, 1439, 1929, 2097, 2427, 2901, 2928
BspMAI	CTGCAG	1 cut(s)	2998
BspMI	ACCTGC	1 cut(s)	1718
BspPI	GGATC	5 cut(s)	519, 581, 782, 1214, 2043
BspT107I	GGYRCC	2 cut(s)	1437, 1927
BsrDI	GCAATG	2 cut(s)	402, 2965
BsrFI	RCCGGY	5 cut(s)	114, 354, 2016, 2885, 3086
BsrGI	TGTACA	1 cut(s)	1542
BsrI	ACTGG	5 cut(s)	966, 1275, 1786, 1827, 3054
BssAI	RCCGGY	5 cut(s)	114, 354, 2016, 2885, 3086
BssECI	CCNNGG	6 cut(s)	599, 1167, 1288, 1890, 2549, 2550
BssMI	GATC	9 cut(s)	511, 586, 787, 1219, 2035, 2059, 2086, 2236, 2761
BssSI	CACGAG	1 cut(s)	1988
BssT1I	CCWWGG	2 cut(s)	599, 1167
Bst2BI	CACGAG	1 cut(s)	1988
Bst2UI	CCWGG	5 cut(s)	1494, 1506, 1925, 2259, 2551
Bst4CI	ACNGT	5 cut(s)	616, 823, 1437, 1602, 2441
Bst6I	CTCTTC	2 cut(s)	1569, 2529
BstAUI	TGTACA	1 cut(s)	1542
BstBAI	YACGTR	2 cut(s)	774, 2567
BstDEI	CTNAG	8 cut(s)	201, 531, 853, 881, 1020, 1470, 1637, 2375
BstDSI	CCRYGG	3 cut(s)	599, 1167, 1288
BstF5I	GGATG	4 cut(s)	336, 1820, 2758, 2775
BstFNI	CGCG	2 cut(s)	210, 1225
BstHHI	GCGC	5 cut(s)	210, 729, 1227, 1715, 2074
BstKTI	GATC	9 cut(s)	514, 589, 790, 1222, 2038, 2062, 2089, 2239, 2764
BstMAI	GTCTC	3 cut(s)	341, 605, 1426
BstMBI	GATC	9 cut(s)	511, 586, 787, 1219, 2035, 2059, 2086, 2236, 2761
BstMWI	GCNNNNNNNGC	8 cut(s)	88, 886, 1064, 1322, 1331, 1594, 2972, 3077
BstNI	CCWGG	5 cut(s)	1494, 1506, 1925, 2259, 2551
BstNSI	RCATGY	5 cut(s)	354, 434, 1346, 1555, 2824
BstPAI	GACNNNNGTC	1 cut(s)	1990
BstSCI	CCNGG	7 cut(s)	1492, 1504, 1889, 1890, 1923, 2257, 2549
BstSFI	CTRYAG	2 cut(s)	1674, 2994
BstSLI	GKGCMC	2 cut(s)	240, 779
BstUI	CGCG	2 cut(s)	210, 1225
BstV2I	GAAGAC	1 cut(s)	708
BstX2I	RGATCY	2 cut(s)	586, 1219
BstXI	CCANNNNNNTGG	1 cut(s)	343
BstYI	RGATCY	2 cut(s)	586, 1219
Bsu15I	ATCGAT	1 cut(s)	786
Bsu36I	CCTNAGG	1 cut(s)	531
BsuI	GTATCC	2 cut(s)	117, 373
BsuRI	GGCC	3 cut(s)	1085, 1646, 2327
BsuTUI	ATCGAT	1 cut(s)	786
BtgI	CCRYGG	3 cut(s)	599, 1167, 1288
BtrI	CACGTC	1 cut(s)	1811
BtsCI	GGATG	4 cut(s)	336, 1820, 2758, 2775
BtsI	GCAGTG	1 cut(s)	238
BtsIMutI	CAGTG	5 cut(s)	238, 1282, 1470, 1598, 1834
BveI	ACCTGC	1 cut(s)	1718
CaiI	CAGNNNCTG	4 cut(s)	191, 530, 1469, 2725
CciI	TCATGA	2 cut(s)	1377, 1905
CfoI	GCGC	5 cut(s)	210, 729, 1227, 1715, 2074
Cfr10I	RCCGGY	5 cut(s)	114, 354, 2016, 2885, 3086
Cfr13I	GGNCC	7 cut(s)	269, 1084, 1292, 1645, 2096, 2425, 2900
Cfr9I	CCCGGG	1 cut(s)	1890
ClaI	ATCGAT	1 cut(s)	786
Csp6I	GTAC	8 cut(s)	57, 771, 935, 1543, 1928, 2395, 2437, 2494
CspAI	ACCGGT	2 cut(s)	2016, 2885
CspCI	CAANNNNNGTGG	4 cut(s)	1587, 1622, 2766, 2801
CviQI	GTAC	8 cut(s)	57, 771, 935, 1543, 1928, 2395, 2437, 2494
DdeI	CTNAG	8 cut(s)	201, 531, 853, 881, 1020, 1470, 1637, 2375
DpnI	GATC	9 cut(s)	513, 588, 789, 1221, 2037, 2061, 2088, 2238, 2763
DpnII	GATC	9 cut(s)	511, 586, 787, 1219, 2035, 2059, 2086, 2236, 2761
DraI	TTTAAA	2 cut(s)	1182, 2406
DraIII	CACNNNGTG	3 cut(s)	451, 914, 1735
Eam1104I	CTCTTC	2 cut(s)	1569, 2529
EarI	CTCTTC	2 cut(s)	1569, 2529
Ecl136II	GAGCTC	1 cut(s)	2975
Eco130I	CCWWGG	2 cut(s)	599, 1167
Eco24I	GRGCYC	2 cut(s)	540, 2977
Eco32I	GATATC	2 cut(s)	1615, 2730
Eco47I	GGWCC	5 cut(s)	269, 1292, 2096, 2425, 2900
Eco53kI	GAGCTC	1 cut(s)	2975
Eco57I	CTGAAG	4 cut(s)	151, 2100, 2702, 2988
Eco81I	CCTNAGG	1 cut(s)	531
Eco88I	CYCGRG	4 cut(s)	10, 1890, 2190, 2895
EcoICRI	GAGCTC	1 cut(s)	2975
EcoO109I	RGGNCCY	3 cut(s)	1084, 1645, 2900
EcoRI	GAATTC	1 cut(s)	1814
EcoRII	CCWGG	5 cut(s)	1492, 1504, 1923, 2257, 2549
EcoRV	GATATC	2 cut(s)	1615, 2730
EcoT14I	CCWWGG	2 cut(s)	599, 1167
EcoT22I	ATGCAT	1 cut(s)	952
EcoT38I	GRGCYC	2 cut(s)	540, 2977
ErhI	CCWWGG	2 cut(s)	599, 1167
Esp3I	CGTCTC	1 cut(s)	1426
FalI	AAGNNNNNCTT	6 cut(s)	625, 657, 2073, 2105, 2961, 2993
FaqI	GGGAC	5 cut(s)	63, 83, 255, 2109, 2913
FauI	CCCGC	3 cut(s)	400, 2025, 2150
FbaI	TGATCA	1 cut(s)	2059
FblI	GTMKAC	1 cut(s)	252
FokI	GGATG	4 cut(s)	323, 1807, 2765, 2782
FriOI	GRGCYC	2 cut(s)	540, 2977
FspBI	CTAG	2 cut(s)	1625, 1977
GlaI	GCGC	5 cut(s)	209, 728, 1226, 1714, 2073
GsuI	CTGGAG	2 cut(s)	1388, 1515
HaeIII	GGCC	3 cut(s)	1085, 1646, 2327
HapII	CCGG	9 cut(s)	115, 289, 355, 1005, 1412, 1891, 2017, 2886, 3087
HhaI	GCGC	5 cut(s)	210, 729, 1227, 1715, 2074
Hin6I	GCGC	5 cut(s)	208, 727, 1225, 1713, 2072
HinP1I	GCGC	5 cut(s)	208, 727, 1225, 1713, 2072
HincII	GTYRAC	3 cut(s)	253, 549, 2639
HindII	GTYRAC	3 cut(s)	253, 549, 2639
HindIII	AAGCTT	3 cut(s)	80, 593, 3020
HpaII	CCGG	9 cut(s)	115, 289, 355, 1005, 1412, 1891, 2017, 2886, 3087
HphI	GGTGA	6 cut(s)	338, 451, 1148, 1678, 2435, 2483
Hpy166II	GTNNAC	6 cut(s)	253, 549, 1543, 2639, 2648, 2738
Hpy8I	GTNNAC	6 cut(s)	253, 549, 1543, 2639, 2648, 2738
HpyCH4III	ACNGT	5 cut(s)	616, 823, 1437, 1602, 2441
HpyCH4IV	ACGT	7 cut(s)	773, 1810, 2111, 2231, 2566, 2583, 2635
HpyF10VI	GCNNNNNNNGC	8 cut(s)	88, 886, 1064, 1322, 1331, 1594, 2972, 3077
HpyF3I	CTNAG	8 cut(s)	201, 531, 853, 881, 1020, 1470, 1637, 2375
HpySE526I	ACGT	7 cut(s)	773, 1810, 2111, 2231, 2566, 2583, 2635
HspAI	GCGC	5 cut(s)	208, 727, 1225, 1713, 2072
Kpn2I	TCCGGA	1 cut(s)	1004
KpnI	GGTACC	1 cut(s)	1931
KroI	GCCGGC	1 cut(s)	3086
KroNI	GCCGGC	1 cut(s)	3088
Ksp22I	TGATCA	1 cut(s)	2059
Kzo9I	GATC	9 cut(s)	511, 586, 787, 1219, 2035, 2059, 2086, 2236, 2761
LmnI	GCTCC	8 cut(s)	88, 535, 724, 763, 1921, 2539, 2972, 3043
MaeI	CTAG	2 cut(s)	1625, 1977
MaeII	ACGT	7 cut(s)	773, 1810, 2111, 2231, 2566, 2583, 2635
MaeIII	GTNAC	8 cut(s)	294, 960, 1345, 1702, 2107, 2441, 2569, 2881
MalI	GATC	9 cut(s)	513, 588, 789, 1221, 2037, 2061, 2088, 2238, 2763
MboI	GATC	9 cut(s)	511, 586, 787, 1219, 2035, 2059, 2086, 2236, 2761
MfeI	CAATTG	3 cut(s)	702, 954, 1964
MflI	RGATCY	2 cut(s)	586, 1219
MhlI	GDGCHC	4 cut(s)	240, 540, 779, 2977
MlyI	GAGTC	4 cut(s)	19, 685, 1017, 2000
MmeI	TCCRAC	4 cut(s)	81, 486, 702, 1059
Mph1103I	ATGCAT	1 cut(s)	952
MroI	TCCGGA	1 cut(s)	1004
MroNI	GCCGGC	1 cut(s)	3086
MroXI	GAANNNNTTC	3 cut(s)	164, 842, 1407
MslI	CAYNNNNRTG	6 cut(s)	341, 772, 930, 1842, 2819, 3005
MspA1I	CMGCKG	3 cut(s)	188, 1316, 2966
MspI	CCGG	9 cut(s)	115, 289, 355, 1005, 1412, 1891, 2017, 2886, 3087
MspR9I	CCNGG	7 cut(s)	1494, 1506, 1891, 1892, 1925, 2259, 2551
MunI	CAATTG	3 cut(s)	702, 954, 1964
MvaI	CCWGG	5 cut(s)	1494, 1506, 1925, 2259, 2551
MvnI	CGCG	2 cut(s)	210, 1225
MwoI	GCNNNNNNNGC	8 cut(s)	88, 886, 1064, 1322, 1331, 1594, 2972, 3077
NaeI	GCCGGC	1 cut(s)	3088
NciI	CCSGG	2 cut(s)	1891, 1892
NcoI	CCATGG	2 cut(s)	599, 1167
NdeII	GATC	9 cut(s)	511, 586, 787, 1219, 2035, 2059, 2086, 2236, 2761
NgoMIV	GCCGGC	1 cut(s)	3086
NlaIV	GGNNCC	9 cut(s)	271, 537, 765, 1439, 1929, 2097, 2427, 2901, 2928
NmeAIII	GCCGAG	1 cut(s)	119
NmuCI	GTSAC	4 cut(s)	1702, 2441, 2569, 2881
NsiI	ATGCAT	1 cut(s)	952
NspI	RCATGY	5 cut(s)	354, 434, 1346, 1555, 2824
PaeI	GCATGC	1 cut(s)	434
PaeR7I	CTCGAG	1 cut(s)	2190
PagI	TCATGA	2 cut(s)	1377, 1905
PasI	CCCWGGG	1 cut(s)	2550
PciI	ACATGT	2 cut(s)	1342, 2820
PcsI	WCGNNNNNNNCGW	1 cut(s)	2035
PdiI	GCCGGC	1 cut(s)	3088
PdmI	GAANNNNTTC	3 cut(s)	164, 842, 1407
PinAI	ACCGGT	2 cut(s)	2016, 2885
PleI	GAGTC	4 cut(s)	19, 685, 1016, 1999
PpsI	GAGTC	4 cut(s)	19, 685, 1016, 1999
Ppu21I	YACGTR	2 cut(s)	774, 2567
PpuMI	RGGWCCY	1 cut(s)	2900
PscI	ACATGT	2 cut(s)	1342, 2820
PshAI	GACNNNNGTC	1 cut(s)	1990
PshBI	ATTAAT	1 cut(s)	897
PsiI	TTATAA	1 cut(s)	2466
Psp124BI	GAGCTC	1 cut(s)	2977
Psp1406I	AACGTT	1 cut(s)	2111
Psp5II	RGGWCCY	1 cut(s)	2900
Psp6I	CCWGG	5 cut(s)	1492, 1504, 1923, 2257, 2549
PspGI	CCWGG	5 cut(s)	1492, 1504, 1923, 2257, 2549
PspN4I	GGNNCC	9 cut(s)	271, 537, 765, 1439, 1929, 2097, 2427, 2901, 2928
PspPI	GGNCC	7 cut(s)	269, 1084, 1292, 1645, 2096, 2425, 2900
PspPPI	RGGWCCY	1 cut(s)	2900
PspXI	VCTCGAGB	1 cut(s)	2190
PstI	CTGCAG	1 cut(s)	2998
PstNI	CAGNNNCTG	4 cut(s)	191, 530, 1469, 2725
PsuI	RGATCY	2 cut(s)	586, 1219
PvuII	CAGCTG	2 cut(s)	1316, 2966
RsaI	GTAC	8 cut(s)	58, 772, 936, 1544, 1929, 2396, 2438, 2495
RsaNI	GTAC	8 cut(s)	57, 771, 935, 1543, 1928, 2395, 2437, 2494
RseI	CAYNNNNRTG	6 cut(s)	341, 772, 930, 1842, 2819, 3005
SacI	GAGCTC	1 cut(s)	2977
SalI	GTCGAC	1 cut(s)	251
Sau3AI	GATC	9 cut(s)	511, 586, 787, 1219, 2035, 2059, 2086, 2236, 2761
Sau96I	GGNCC	7 cut(s)	269, 1084, 1292, 1645, 2096, 2425, 2900
SchI	GAGTC	4 cut(s)	19, 685, 1017, 2000
ScrFI	CCNGG	7 cut(s)	1494, 1506, 1891, 1892, 1925, 2259, 2551
SduI	GDGCHC	4 cut(s)	240, 540, 779, 2977
SfcI	CTRYAG	2 cut(s)	1674, 2994
Sfr274I	CTCGAG	1 cut(s)	2190
SinI	GGWCC	5 cut(s)	269, 1292, 2096, 2425, 2900
SlaI	CTCGAG	1 cut(s)	2190
SmaI	CCCGGG	1 cut(s)	1892
SmiI	ATTTAAAT	1 cut(s)	2406
SmiMI	CAYNNNNRTG	6 cut(s)	341, 772, 930, 1842, 2819, 3005
SmlI	CTYRAG	1 cut(s)	2190
SmoI	CTYRAG	1 cut(s)	2190
SphI	GCATGC	1 cut(s)	434
SsiI	CCGC	8 cut(s)	112, 143, 188, 407, 1223, 1325, 2032, 2157
SspI	AATATT	1 cut(s)	1953
SspMI	CTAG	2 cut(s)	1625, 1977
SstI	GAGCTC	1 cut(s)	2977
StyD4I	CCNGG	7 cut(s)	1492, 1504, 1889, 1890, 1923, 2257, 2549
StyI	CCWWGG	2 cut(s)	599, 1167
SwaI	ATTTAAAT	1 cut(s)	2406
TaaI	ACNGT	5 cut(s)	616, 823, 1437, 1602, 2441
TaiI	ACGT	7 cut(s)	776, 1813, 2114, 2234, 2569, 2586, 2638
TaqI	TCGA	5 cut(s)	23, 252, 786, 1130, 2191
TaqII	GACCGA	2 cut(s)	2113, 2997
TatI	WGTACW	4 cut(s)	934, 1542, 2394, 2493
TauI	GCSGC	3 cut(s)	146, 191, 1328
TscAI	CASTG	5 cut(s)	245, 1282, 1470, 1605, 1834
TseFI	GTSAC	4 cut(s)	1702, 2441, 2569, 2881
Tsp45I	GTSAC	4 cut(s)	1702, 2441, 2569, 2881
TspGWI	ACGGA	5 cut(s)	67, 237, 1305, 1415, 2782
TspMI	CCCGGG	1 cut(s)	1890
TspRI	CASTG	5 cut(s)	245, 1282, 1470, 1605, 1834
VpaK11BI	GGWCC	5 cut(s)	269, 1292, 2096, 2425, 2900
VspI	ATTAAT	1 cut(s)	897
XapI	RAATTY	6 cut(s)	813, 1423, 1814, 2123, 2867, 2876
XceI	RCATGY	5 cut(s)	354, 434, 1346, 1555, 2824
XcmI	CCANNNNNNNNNTGG	1 cut(s)	433
XhoI	CTCGAG	1 cut(s)	2190
XmaI	CCCGGG	1 cut(s)	1890
XmiI	GTMKAC	1 cut(s)	252
XmnI	GAANNNNTTC	3 cut(s)	164, 842, 1407
XspI	CTAG	2 cut(s)	1625, 1977
Zsp2I	ATGCAT	1 cut(s)	952

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.