MD16G1148200.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008558
Physical Location & Seq
Reverse (-)
11548497 .. 11561271
12775 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1148200.v1.1.491

Sequence Viewer

Length: 3102 bp
ATGTTCTTCTCGGGCGATTCATCGACTCGGAAGCGAGTTGATTTGGGAGGGCGGAGTACGAAGGAAAGGGACAGGCAGAAGCTTTTGGAGCAGACAAGGTTGGAGCGCAACCGCCGGTTGTGGATACGGCAGCAGAACTTAGCCGCGTTGAAAATCCAGAAATGCTTTAGAGGGAGGAAGGTGGCAGCGGCTGAACATTCTAAGGTGCGTGAGCAGTTCTATGGAAGATATGGGAGGCATTGCCAGAATGTGGACAGATTTAGTTTTGGTCCTGATTCAGAGTTTCTCCGCCAGTTACTTTTCTTCTTTGATGCTCGAAGCGTTGGTGATTTTTCTATCCTTGTGGAGACGTGCCGGTTGCTTCATCAATTTGTCAGAGAGACAGGGGATATTATTAGCCTCGTTGCAGGCATGGATTATTCGTCCAAGCATGCTTTGGTGAAACACAGAGTGAAGCAACTTGCATATATCTGTATGAAGGCTGTTCATCAAAACAGAAATCAGTTGAAGGATCAACTTTTTGCAGCCCCTGAGGAGGCCACCACGTCAACAACTTTACTGTTAGAAGCATTAGTTTTATTGATAGATCCCAAGCTTCCATGGGCTTGTGAGACAGTTGGCTATCTTTTGCAAAGAAAGGCTTTTATCCTGTATAGAGAGATTATTTTAACAGGAAAGGAAAGTATAAAGACTCATAACTACATTGGCAGAGTGTCATCGTTGGAGCGTAGTCTTGCTGTTGTAATTTCTCACATTGGTCAGGAGCCATGTACGTGCCCAAACATCGATCCACATTGGAGTTTTTCATCTCAAATTCTTACAGTTCCATTTGTATGGAAGCTATTTCCCTACTTAGGAGAGGTTTTTGCAAGACGAGGGCTGAGCCAGCATTATATTAATCAGATGTCACTCTGTGTGAAAAATCATGCTGATGTACTTCCGAAAGATGCATCAGTTGAGTTACCCGGTTATGCCTGCCTTATTGGAAATATATTGGAATCTTCCGGGGTCGCTCTGTCTCAGACTGATTGCTCATTTCAAATGGCTTTAGACCTTGCTGGCGTCGCAACATTCTTGTTGGAGGCCCTTCCTTCAATGAAATCATCTAATAGAGAAAGCAAAGAAGATTCTATGGGTGATGATGATATGATTGAAGGAGATGATGCAATGGAAATTTGTTTGAATAATGATTTAGAACGACAGATATGTGATGCCATAGATCTGCGCTTTCTTCTGCAATTAACAAATGTTTTGTTTGGAGGGATTTCACCTTCCAGTGGCTTGGACCACAGACCGGATGATAAAGAGGTGTCAGCTGTTGGTGCGGCTTGTGCTTTTCTACATGTTACTTTAAACACCTTACCTCTGGAGAGAATCATGACTATACTAGCTTATAGAACAGAACTTGTTCCGGTGCTTTGGAATTTTATGAAACGGTGCCATGAAAACCAGAAATGGCAGTCAGTATCTGAGCAGTTGGCATATCTGCTGCCCGGAGATGCACCTGGCTGGCTATTACCTTTGGCTGTATTCTGTCCTGTGTACAAGCACATGCTTACAGTAGTTGATAATGAGGAGTTCTATGAGCAGGAGAAGCCACTATCATTGAAGGATATCAGATACCTAGTTATCATACTCAGACAGGCCCTATGGCAACTTCTTTGGGTGAATCCTACGGCTCCCAGTAATTCTTCGAAACCTGTCACAACCTCCTCTTCTAAGAAGCACCCTGTGGAGTTAATTCAACATAGAGTTAGCATTGTAGCTTCTGAACTCCTCTCCCAGTTGCAAGATTGGAACAATAGACGAGAATTCACATCCCCCAGTGACTTTCATGCTGATGGTGTCAATGAGTTCTTTATTACACAGGCCGTAATAGAAAATACCCGAGCAAATGACATCATGAAGCAAGCTCCCTTCCTGGTACCATTCACAAGCAGAGTTAAAATATTCACTTCACAATTGGCAGCAGCTAGACAAAGACATGAGTCTAATTCTGTATTTACCAGAAACCGATTCAGAATACGGCGGGATCGTATCTTGGAAGATGCTTACGACCAAATGAGTGCGCTGTCTGAAGATGATCTTCGGGGACCGATTCGTGTAACGTTTGTAAATGAATTTGGGGTTGAGGAGGCTGGAATTGATGGCGGTGGGATTTTCAAAGACTTTATGGAGAACATTACTCAGGCAGCCTTTGATGTTCAGTATGGATTGTTTAAGGAAACATCCGATCATTTACTCTACCCTAGTCCTGGTTCAGGAATGATTCACGAACAGCATCTCCGATTCTTTCACTTTCTTGGGATTCTTCTTGCAAAGGCCATGTTCGAGGGGATTCTTGTTGATATACCATTTGCTACGTTTTTTCTGAGCAAATTAAAACAAAAGTACAACTATTTAAATGATTTGCCTTCATTGGACCCAGAGTTGTACCGTCACCTTATTTTCTTGAAGCGTTATAAAGGTGATATATCAGAATTAGAATTGTACTTTGTCATTGTAAATAATGAATATGGAGAGCAAACAGAAGAGGAGCTGCTTCCCGGGGGAAAAAACCTACGTGTCACTAATGAGAATGTCATTACCTTTATTCATCTTGTTGCCAACCATCGTTTGAACTTTCAGATACGTCAACAAAGTTCACATTTCTTAAGGGGGTTTCAGCAGCTTATACCGAAAGATTGGATAGATATGTTTAATGAGCATGAACTTCAGCTTCTGATATCAGGTTCACTTGACAGCTTGGATGTCGATGACCTACGGATGCACACAAACTATGTGGGTGGCTATCATAGTGAGCATTATGTCATTGACATGTTCTGGGAAGTTCTGAAAAGTTTTTCATTGGAAAATCAGAAGAAATTTCTGAAATTTGTGACCGGGTGCTCTCGAGGACCTCTGCTTGGATTCAAACACTTGGAACCATTATTTTGCATACAGAGAGCTGGTGGCAATGGAGCTGAAGGAGCTCTTGACCGATTACCGACTGCAGCCACTTGTATGAATCTCCTAAAGCTTCCACCATATAGAAGTAAGGAGCAACTGGAATCAAAATTGATGTATGCTATAAGCGCAGATGCCGGGTTTGATTTGAGCTGA

Protein Analysis

1034

Amino Acids

118.01

Weight (kDa)

6.32

Isoelectric Point (pI)

45.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HECT PF00632 728 - 1033 7.2e-94 HECT-domain (ubiquitin-transferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2463
Acc65I GGTACC 1 cut(s) 1924
AccB1I GGYRCC 2 cut(s) 1437, 1924
AccB7I CCANNNNNTGG 1 cut(s) 250
AccII CGCG 1 cut(s) 146
AciI CCGC 8 cut(s) 52, 112, 144, 188, 289, 1325, 2029, 2151
AclI AACGTT 1 cut(s) 2108
AclWI GGATC 4 cut(s) 519, 581, 782, 2040
AcsI RAATTY 7 cut(s) 813, 1173, 1423, 1811, 2120, 2864, 2873
AcuI CTGAAG 3 cut(s) 2097, 2699, 2985
AcyI GRCGYC 1 cut(s) 1062
AdeI CACNNNGTG 3 cut(s) 451, 914, 1732
AfaI GTAC 8 cut(s) 58, 772, 936, 1544, 1926, 2393, 2435, 2492
AfiI CCNNNNNNNGG 8 cut(s) 250, 535, 854, 1277, 1294, 2255, 2261, 2906
AflII CTTAAG 1 cut(s) 2653
AflIII ACRYGT 3 cut(s) 1342, 2563, 2817
AjiI CACGTC 2 cut(s) 351, 546
AjnI CCWGG 3 cut(s) 1504, 1920, 2254
AloI GAACNNNNNNTCC 2 cut(s) 2268, 2300
Alw21I GWGCWC 2 cut(s) 2891, 2974
Alw26I GTCTC 4 cut(s) 341, 374, 605, 1023
AlwI GGATC 4 cut(s) 519, 581, 782, 2040
AlwNI CAGNNNCTG 4 cut(s) 191, 530, 1469, 2722
Ama87I CYCGRG 4 cut(s) 10, 1887, 2546, 2892
AoxI GGCC 5 cut(s) 537, 1083, 1644, 1869, 2322
ApoI RAATTY 7 cut(s) 813, 1173, 1423, 1811, 2120, 2864, 2873
AseI ATTAAT 1 cut(s) 897
Asp700I GAANNNNTTC 1 cut(s) 1407
Asp718I GGTACC 1 cut(s) 1924
AspLEI GCGC 4 cut(s) 108, 1227, 2071, 3077
AspS9I GGNCC 7 cut(s) 269, 1084, 1285, 1645, 2093, 2422, 2897
AsuC2I CCSGG 7 cut(s) 966, 1006, 1494, 2547, 2548, 2884, 3085
AsuHPI GGTGA 7 cut(s) 338, 451, 1148, 1260, 1678, 2432, 2480
AsuII TTCGAA 1 cut(s) 1694
AvaI CYCGRG 4 cut(s) 10, 1887, 2546, 2892
AvaII GGWCC 5 cut(s) 269, 1285, 2093, 2422, 2897
AxyI CCTNAGG 1 cut(s) 531
BaeGI GKGCMC 1 cut(s) 779
BanI GGYRCC 2 cut(s) 1437, 1924
BanII GRGCYC 1 cut(s) 2974
Bbv12I GWGCWC 2 cut(s) 2891, 2974
BccI CCATC 3 cut(s) 1835, 2141, 2619
BceAI ACGGC 4 cut(s) 143, 1692, 1856, 2042
BciT130I CCWGG 3 cut(s) 1506, 1922, 2256
BciVI GTATCC 1 cut(s) 117
BcnI CCSGG 7 cut(s) 966, 1006, 1494, 2547, 2548, 2884, 3085
BcoDI GTCTC 4 cut(s) 341, 374, 605, 1023
BfaI CTAG 4 cut(s) 1388, 1625, 1974, 2250
BfmI CTRYAG 1 cut(s) 2991
BfrI CTTAAG 1 cut(s) 2653
BfuI GTATCC 1 cut(s) 117
BglII AGATCT 1 cut(s) 1219
BlpI GCTNAGC 1 cut(s) 881
Bme18I GGWCC 5 cut(s) 269, 1285, 2093, 2422, 2897
BmeT110I CYCGRG 4 cut(s) 10, 1887, 2546, 2892
BmgBI CACGTC 2 cut(s) 351, 546
BmgT120I GGNCC 7 cut(s) 269, 1084, 1285, 1645, 2093, 2422, 2897
BmiI GGNNCC 7 cut(s) 765, 1439, 1680, 1926, 2094, 2424, 2925
BmrI ACTGGG 3 cut(s) 1677, 1777, 1818
BmuI ACTGGG 3 cut(s) 1677, 1777, 1818
BoxI GACNNNNGTC 1 cut(s) 1987
BpmI CTGGAG 1 cut(s) 1388
Bpu1102I GCTNAGC 1 cut(s) 881
Bpu14I TTCGAA 1 cut(s) 1694
BpuMI CCSGG 7 cut(s) 966, 1006, 1494, 2547, 2548, 2884, 3085
Bsa29I ATCGAT 1 cut(s) 786
BsaAI YACGTR 2 cut(s) 774, 2564
BsaHI GRCGYC 1 cut(s) 1062
BsaJI CCNNGG 4 cut(s) 599, 1005, 2546, 2547
BsaWI WCCGGW 2 cut(s) 1294, 1411
BsaXI ACNNNNNCTCC 2 cut(s) 2268, 2298
Bsc4I CCNNNNNNNGG 8 cut(s) 250, 535, 854, 1277, 1294, 2255, 2261, 2906
Bse118I RCCGGY 2 cut(s) 114, 354
Bse1I ACTGG 6 cut(s) 292, 1275, 1683, 1783, 1824, 3051
Bse21I CCTNAGG 1 cut(s) 531
Bse3DI GCAATG 3 cut(s) 238, 1173, 2962
BseBI CCWGG 3 cut(s) 1506, 1922, 2256
BseCI ATCGAT 1 cut(s) 786
BseDI CCNNGG 4 cut(s) 599, 1005, 2546, 2547
BseGI GGATG 5 cut(s) 1303, 1817, 2228, 2755, 2772
BseLI CCNNNNNNNGG 8 cut(s) 250, 535, 854, 1277, 1294, 2255, 2261, 2906
BseMI GCAATG 3 cut(s) 238, 1173, 2962
BseMII CTCAG 7 cut(s) 522, 872, 1034, 1461, 1651, 2201, 2363
BseNI ACTGG 6 cut(s) 292, 1275, 1683, 1783, 1824, 3051
BseRI GAGGAG 6 cut(s) 548, 1589, 1702, 1766, 2147, 2549
BseSI GKGCMC 1 cut(s) 779
Bsh1236I CGCG 1 cut(s) 146
BshFI GGCC 5 cut(s) 539, 1085, 1646, 1871, 2324
BshNI GGYRCC 2 cut(s) 1437, 1924
BshVI ATCGAT 1 cut(s) 786
BsiHKAI GWGCWC 2 cut(s) 2891, 2974
BsiHKCI CYCGRG 4 cut(s) 10, 1887, 2546, 2892
BslFI GGGAC 2 cut(s) 83, 2106
BslI CCNNNNNNNGG 8 cut(s) 250, 535, 854, 1277, 1294, 2255, 2261, 2906
BsmAI GTCTC 4 cut(s) 341, 374, 605, 1023
BsmBI CGTCTC 1 cut(s) 341
BsmFI GGGAC 2 cut(s) 83, 2106
BsnI GGCC 5 cut(s) 539, 1085, 1646, 1871, 2324
BsoBI CYCGRG 4 cut(s) 10, 1887, 2546, 2892
Bsp119I TTCGAA 1 cut(s) 1694
Bsp1286I GDGCHC 3 cut(s) 779, 2891, 2974
Bsp1407I TGTACA 1 cut(s) 1542
Bsp143I GATC 7 cut(s) 511, 586, 787, 1219, 2032, 2083, 2233
Bsp1720I GCTNAGC 1 cut(s) 881
Bsp19I CCATGG 1 cut(s) 599
BspACI CCGC 8 cut(s) 52, 112, 144, 188, 289, 1325, 2029, 2151
BspANI GGCC 5 cut(s) 539, 1085, 1646, 1871, 2324
BspCNI CTCAG 7 cut(s) 523, 873, 1033, 1462, 1650, 2200, 2364
BspDI ATCGAT 1 cut(s) 786
BspFNI CGCG 1 cut(s) 146
BspHI TCATGA 2 cut(s) 1377, 1902
BspLI GGNNCC 7 cut(s) 765, 1439, 1680, 1926, 2094, 2424, 2925
BspMAI CTGCAG 1 cut(s) 2995
BspPI GGATC 4 cut(s) 519, 581, 782, 2040
BspT104I TTCGAA 1 cut(s) 1694
BspT107I GGYRCC 2 cut(s) 1437, 1924
BspTI CTTAAG 1 cut(s) 2653
BsrDI GCAATG 3 cut(s) 238, 1173, 2962
BsrFI RCCGGY 2 cut(s) 114, 354
BsrGI TGTACA 1 cut(s) 1542
BsrI ACTGG 6 cut(s) 292, 1275, 1683, 1783, 1824, 3051
BssAI RCCGGY 2 cut(s) 114, 354
BssECI CCNNGG 4 cut(s) 599, 1005, 2546, 2547
BssMI GATC 7 cut(s) 511, 586, 787, 1219, 2032, 2083, 2233
BssNI GRCGYC 1 cut(s) 1062
BssT1I CCWWGG 1 cut(s) 599
Bst2UI CCWGG 3 cut(s) 1506, 1922, 2256
Bst4CI ACNGT 6 cut(s) 561, 616, 823, 1437, 1561, 2438
Bst6I CTCTTC 2 cut(s) 1720, 2526
BstACI GRCGYC 1 cut(s) 1062
BstAFI CTTAAG 1 cut(s) 2653
BstAUI TGTACA 1 cut(s) 1542
BstBAI YACGTR 2 cut(s) 774, 2564
BstBI TTCGAA 1 cut(s) 1694
BstC8I GCNNGC 7 cut(s) 409, 432, 887, 976, 1060, 1511, 1911
BstDSI CCRYGG 1 cut(s) 599
BstENI CCTNNNNNAGG 1 cut(s) 2259
BstF5I GGATG 5 cut(s) 1303, 1817, 2228, 2755, 2772
BstFNI CGCG 1 cut(s) 146
BstHHI GCGC 4 cut(s) 108, 1227, 2071, 3077
BstKTI GATC 7 cut(s) 514, 589, 790, 1222, 2035, 2086, 2236
BstMAI GTCTC 4 cut(s) 341, 374, 605, 1023
BstMBI GATC 7 cut(s) 511, 586, 787, 1219, 2032, 2083, 2233
BstMWI GCNNNNNNNGC 8 cut(s) 88, 886, 1064, 1322, 1331, 1594, 2969, 3074
BstNI CCWGG 3 cut(s) 1506, 1922, 2256
BstNSI RCATGY 4 cut(s) 434, 1346, 1555, 2821
BstPAI GACNNNNGTC 1 cut(s) 1987
BstSFI CTRYAG 1 cut(s) 2991
BstSLI GKGCMC 1 cut(s) 779
BstUI CGCG 1 cut(s) 146
BstX2I RGATCY 2 cut(s) 586, 1219
BstXI CCANNNNNNTGG 2 cut(s) 834, 1282
BstYI RGATCY 2 cut(s) 586, 1219
Bsu15I ATCGAT 1 cut(s) 786
Bsu36I CCTNAGG 1 cut(s) 531
BsuI GTATCC 1 cut(s) 117
BsuRI GGCC 5 cut(s) 539, 1085, 1646, 1871, 2324
BsuTUI ATCGAT 1 cut(s) 786
BtgI CCRYGG 1 cut(s) 599
BtrI CACGTC 2 cut(s) 351, 546
BtsCI GGATG 5 cut(s) 1303, 1817, 2228, 2755, 2772
BtsIMutI CAGTG 2 cut(s) 1282, 1831
Cac8I GCNNGC 7 cut(s) 409, 432, 887, 976, 1060, 1511, 1911
CaiI CAGNNNCTG 4 cut(s) 191, 530, 1469, 2722
CciI TCATGA 2 cut(s) 1377, 1902
CfoI GCGC 4 cut(s) 108, 1227, 2071, 3077
Cfr10I RCCGGY 2 cut(s) 114, 354
Cfr13I GGNCC 7 cut(s) 269, 1084, 1285, 1645, 2093, 2422, 2897
Cfr9I CCCGGG 1 cut(s) 2546
ClaI ATCGAT 1 cut(s) 786
CseI GACGC 1 cut(s) 1051
Csp6I GTAC 8 cut(s) 57, 771, 935, 1543, 1925, 2392, 2434, 2491
CspCI CAANNNNNGTGG 4 cut(s) 1587, 1622, 2763, 2798
CviQI GTAC 8 cut(s) 57, 771, 935, 1543, 1925, 2392, 2434, 2491
DpnI GATC 7 cut(s) 513, 588, 789, 1221, 2034, 2085, 2235
DpnII GATC 7 cut(s) 511, 586, 787, 1219, 2032, 2083, 2233
DraI TTTAAA 2 cut(s) 1353, 2403
DraIII CACNNNGTG 3 cut(s) 451, 914, 1732
Eam1104I CTCTTC 2 cut(s) 1720, 2526
EarI CTCTTC 2 cut(s) 1720, 2526
EciI GGCGGA 2 cut(s) 67, 278
Ecl136II GAGCTC 1 cut(s) 2972
Eco130I CCWWGG 1 cut(s) 599
Eco24I GRGCYC 1 cut(s) 2974
Eco32I GATATC 2 cut(s) 1615, 2727
Eco47I GGWCC 5 cut(s) 269, 1285, 2093, 2422, 2897
Eco53kI GAGCTC 1 cut(s) 2972
Eco57I CTGAAG 3 cut(s) 2097, 2699, 2985
Eco81I CCTNAGG 1 cut(s) 531
Eco88I CYCGRG 4 cut(s) 10, 1887, 2546, 2892
EcoICRI GAGCTC 1 cut(s) 2972
EcoNI CCTNNNNNAGG 1 cut(s) 2259
EcoO109I RGGNCCY 3 cut(s) 1084, 1645, 2897
EcoRI GAATTC 1 cut(s) 1811
EcoRII CCWGG 3 cut(s) 1504, 1920, 2254
EcoRV GATATC 2 cut(s) 1615, 2727
EcoT14I CCWWGG 1 cut(s) 599
EcoT22I ATGCAT 1 cut(s) 952
EcoT38I GRGCYC 1 cut(s) 2974
ErhI CCWWGG 1 cut(s) 599
Esp3I CGTCTC 1 cut(s) 341
FalI AAGNNNNNCTT 6 cut(s) 625, 657, 2070, 2102, 2958, 2990
FaqI GGGAC 2 cut(s) 83, 2106
FauI CCCGC 1 cut(s) 2022
FokI GGATG 5 cut(s) 1310, 1804, 2215, 2762, 2779
FriOI GRGCYC 1 cut(s) 2974
FspBI CTAG 4 cut(s) 1388, 1625, 1974, 2250
GlaI GCGC 4 cut(s) 107, 1226, 2070, 3076
GsuI CTGGAG 1 cut(s) 1388
HaeIII GGCC 5 cut(s) 539, 1085, 1646, 1871, 2324
HgaI GACGC 1 cut(s) 1051
HhaI GCGC 4 cut(s) 108, 1227, 2071, 3077
Hin1I GRCGYC 1 cut(s) 1062
Hin6I GCGC 4 cut(s) 106, 1225, 2069, 3075
HinP1I GCGC 4 cut(s) 106, 1225, 2069, 3075
HincII GTYRAC 2 cut(s) 549, 2636
HindII GTYRAC 2 cut(s) 549, 2636
HindIII AAGCTT 3 cut(s) 80, 593, 3017
HphI GGTGA 7 cut(s) 338, 451, 1148, 1260, 1678, 2432, 2480
Hpy166II GTNNAC 6 cut(s) 253, 549, 1543, 2636, 2645, 2735
Hpy8I GTNNAC 6 cut(s) 253, 549, 1543, 2636, 2645, 2735
Hpy99I CGWCG 1 cut(s) 1067
HpyCH4III ACNGT 6 cut(s) 561, 616, 823, 1437, 1561, 2438
HpyCH4IV ACGT 7 cut(s) 350, 545, 773, 2108, 2363, 2563, 2632
HpyF10VI GCNNNNNNNGC 8 cut(s) 88, 886, 1064, 1322, 1331, 1594, 2969, 3074
HpySE526I ACGT 7 cut(s) 350, 545, 773, 2108, 2363, 2563, 2632
Hsp92I GRCGYC 1 cut(s) 1062
HspAI GCGC 4 cut(s) 106, 1225, 2069, 3075
KpnI GGTACC 1 cut(s) 1928
Kzo9I GATC 7 cut(s) 511, 586, 787, 1219, 2032, 2083, 2233
MaeI CTAG 4 cut(s) 1388, 1625, 1974, 2250
MaeII ACGT 7 cut(s) 350, 545, 773, 2108, 2363, 2563, 2632
MalI GATC 7 cut(s) 513, 588, 789, 1221, 2034, 2085, 2235
MboI GATC 7 cut(s) 511, 586, 787, 1219, 2032, 2083, 2233
MfeI CAATTG 1 cut(s) 1961
MflI RGATCY 2 cut(s) 586, 1219
MhlI GDGCHC 3 cut(s) 779, 2891, 2974
MlyI GAGTC 3 cut(s) 19, 685, 1997
MmeI TCCRAC 3 cut(s) 81, 702, 1059
Mph1103I ATGCAT 1 cut(s) 952
MroXI GAANNNNTTC 1 cut(s) 1407
MslI CAYNNNNRTG 6 cut(s) 772, 832, 930, 1839, 2816, 3002
MspA1I CMGCKG 2 cut(s) 188, 1316
MspCI CTTAAG 1 cut(s) 2653
MunI CAATTG 1 cut(s) 1961
MvaI CCWGG 3 cut(s) 1506, 1922, 2256
MvnI CGCG 1 cut(s) 146
MwoI GCNNNNNNNGC 8 cut(s) 88, 886, 1064, 1322, 1331, 1594, 2969, 3074
NciI CCSGG 7 cut(s) 966, 1006, 1494, 2547, 2548, 2884, 3085
NcoI CCATGG 1 cut(s) 599
NdeII GATC 7 cut(s) 511, 586, 787, 1219, 2032, 2083, 2233
NlaIV GGNNCC 7 cut(s) 765, 1439, 1680, 1926, 2094, 2424, 2925
NmuCI GTSAC 6 cut(s) 906, 1702, 1826, 2438, 2566, 2878
NsiI ATGCAT 1 cut(s) 952
NspI RCATGY 4 cut(s) 434, 1346, 1555, 2821
NspV TTCGAA 1 cut(s) 1694
PaeI GCATGC 1 cut(s) 434
PaeR7I CTCGAG 1 cut(s) 2892
PagI TCATGA 2 cut(s) 1377, 1902
PciI ACATGT 2 cut(s) 1342, 2817
PcsI WCGNNNNNNNCGW 1 cut(s) 2032
PdmI GAANNNNTTC 1 cut(s) 1407
PflMI CCANNNNNTGG 1 cut(s) 250
PleI GAGTC 3 cut(s) 19, 685, 1996
PpsI GAGTC 3 cut(s) 19, 685, 1996
Ppu21I YACGTR 2 cut(s) 774, 2564
PpuMI RGGWCCY 1 cut(s) 2897
PscI ACATGT 2 cut(s) 1342, 2817
PshAI GACNNNNGTC 1 cut(s) 1987
PshBI ATTAAT 1 cut(s) 897
PsiI TTATAA 1 cut(s) 2463
Psp124BI GAGCTC 1 cut(s) 2974
Psp1406I AACGTT 1 cut(s) 2108
Psp5II RGGWCCY 1 cut(s) 2897
Psp6I CCWGG 3 cut(s) 1504, 1920, 2254
PspGI CCWGG 3 cut(s) 1504, 1920, 2254
PspN4I GGNNCC 7 cut(s) 765, 1439, 1680, 1926, 2094, 2424, 2925
PspPI GGNCC 7 cut(s) 269, 1084, 1285, 1645, 2093, 2422, 2897
PspPPI RGGWCCY 1 cut(s) 2897
PstI CTGCAG 1 cut(s) 2995
PstNI CAGNNNCTG 4 cut(s) 191, 530, 1469, 2722
PsuI RGATCY 2 cut(s) 586, 1219
PvuII CAGCTG 1 cut(s) 1316
RsaI GTAC 8 cut(s) 58, 772, 936, 1544, 1926, 2393, 2435, 2492
RsaNI GTAC 8 cut(s) 57, 771, 935, 1543, 1925, 2392, 2434, 2491
RseI CAYNNNNRTG 6 cut(s) 772, 832, 930, 1839, 2816, 3002
SacI GAGCTC 1 cut(s) 2974
Sau3AI GATC 7 cut(s) 511, 586, 787, 1219, 2032, 2083, 2233
Sau96I GGNCC 7 cut(s) 269, 1084, 1285, 1645, 2093, 2422, 2897
SchI GAGTC 3 cut(s) 19, 685, 1997
SduI GDGCHC 3 cut(s) 779, 2891, 2974
SfcI CTRYAG 1 cut(s) 2991
Sfr274I CTCGAG 1 cut(s) 2892
SfuI TTCGAA 1 cut(s) 1694
SinI GGWCC 5 cut(s) 269, 1285, 2093, 2422, 2897
SlaI CTCGAG 1 cut(s) 2892
SmaI CCCGGG 1 cut(s) 2548
SmiI ATTTAAAT 1 cut(s) 2403
SmiMI CAYNNNNRTG 6 cut(s) 772, 832, 930, 1839, 2816, 3002
SmlI CTYRAG 2 cut(s) 2653, 2892
SmoI CTYRAG 2 cut(s) 2653, 2892
SphI GCATGC 1 cut(s) 434
SsiI CCGC 8 cut(s) 52, 112, 144, 188, 289, 1325, 2029, 2151
SspI AATATT 1 cut(s) 1950
SspMI CTAG 4 cut(s) 1388, 1625, 1974, 2250
SstI GAGCTC 1 cut(s) 2974
StyI CCWWGG 1 cut(s) 599
SwaI ATTTAAAT 1 cut(s) 2403
TaaI ACNGT 6 cut(s) 561, 616, 823, 1437, 1561, 2438
TaiI ACGT 7 cut(s) 353, 548, 776, 2111, 2366, 2566, 2635
TaqI TCGA 7 cut(s) 23, 316, 786, 1694, 2331, 2754, 2893
TaqII GACCGA 2 cut(s) 2110, 2994
TatI WGTACW 4 cut(s) 934, 1542, 2391, 2490
TauI GCSGC 3 cut(s) 146, 191, 1328
TscAI CASTG 2 cut(s) 1282, 1831
TseFI GTSAC 6 cut(s) 906, 1702, 1826, 2438, 2566, 2878
Tsp45I GTSAC 6 cut(s) 906, 1702, 1826, 2438, 2566, 2878
TspGWI ACGGA 1 cut(s) 2779
TspMI CCCGGG 1 cut(s) 2546
TspRI CASTG 2 cut(s) 1282, 1831
Van91I CCANNNNNTGG 1 cut(s) 250
Vha464I CTTAAG 1 cut(s) 2653
VpaK11BI GGWCC 5 cut(s) 269, 1285, 2093, 2422, 2897
VspI ATTAAT 1 cut(s) 897
XagI CCTNNNNNAGG 1 cut(s) 2259
XapI RAATTY 7 cut(s) 813, 1173, 1423, 1811, 2120, 2864, 2873
XceI RCATGY 4 cut(s) 434, 1346, 1555, 2821
XcmI CCANNNNNNNNNTGG 1 cut(s) 433
XhoI CTCGAG 1 cut(s) 2892
XmaI CCCGGG 1 cut(s) 2546
XmnI GAANNNNTTC 1 cut(s) 1407
XspI CTAG 4 cut(s) 1388, 1625, 1974, 2250
Zsp2I ATGCAT 1 cut(s) 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.