Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0014718

Physical Location & Seq

Genomic Coordinates Reverse (-)

11807823 .. 11809934

Gene Structure 2112 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1150700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1158 bp

ATGGATGGTAGAGAAGAAGAACTAAAGACCACCGATCGTAGCCACAGCATGGCAAATTCGGCAGTTTTCTCCGACGAGATTCCGACCAACTTTCCTTCCTTTTCCGGCATATTCGACATGCCATCTTCGTGCGAAGATCAGAAGGGTTCTCTTGGAGGGTTCATGGACTTGCTCGTAATCCCAGACTACGCTCCTTCTCTATTCGATTTCCCTCAAACTACGTCGTCGCTCATGATGATGCCACCTCACCACAATCAGCAACGATCACTTCCCGCTCCAGCATCTACTTCTACAGTAGTAGCGATGCCGGAGAGGGAGAGTGCTACTACTACTAATACAACCTCGGAGGTTTTGAACAGCAGTACGGCTCCGACGACGCCGAACTCGTCTTCGATCTCTTCGTCGTCGAATGAAGCTGCGGCGAATAATAATGAGGGCCAAATGAAAAAAGTGCAGGAAGAGGAAGCCGATGAGCAAGATCCGGAGAAAACCCAGAAACAATTGAAACCGAAAAAGAAGAACCAAAAAAGGCAGAGGGAGCCGAGATTTGCGTTCATGACAAAGAGTGAGGTTGATAACCTAGATGATGGCTACCGATGGCGAAAATACGGCCAAAAAGCTGTCAAAAATAGTCCTTATCCCAGGAGCTATTATCGTTGCACCACTGCGGGCTGTGGTGTGAAGAAGAGGGTGGAGAGGTCGTCCGACGACCCGTCCACTGTGGTCACCACATACGAAGGCCAACACACGCATCCAAGTCCCATTACACCGCGTGGCACAATGGGAATTGCACCACTGCCGCATCAGTCTACTGGGTTTATATCTGCTGCGGAGGCATCATCAAATCCCTTTGGAGTTCAGCAGTTTTTACCTCATCATCATCACTATCAACAACAGCAGCAACAGCAATATGCCTATATGTATCAATCCTCCCCTTCTTTGAATATCAGCTCTTCCACCTACGGTGGTGGTGCTGGTGGCGGTCCTTTTAATCCCTCCTCATTTTCTACTAGTTTACTTCAAGAGAGATATAATTTTGAAGGACCCCCTTCTTCTTCGGCTACCAATTTTCTTAGAGACCATGGACTTCTTCAGGATATCGTGCCGCAGATTCGAAAGGAGCCCAAAGAAGAAGATCATCATCATCAACATCAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009891 GO:0009893 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

386

Amino Acids

42.7

Weight (kDa)

6.11

Isoelectric Point (pI)

66.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	195 - 252	5.6e-28	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014718)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g20230
malus_domestica	MD13G1150700.v1.1 MD16G1151000.v1.1
prunus_persica	Prupe.1G114800_v2.0.a1
pyrus_communis	pycom13g12950 pycom16g12920
rosa_chinensis	RchiOBHm_Chr4g0425801
rosa_laevigata	RLG00000007339
rosa_multiflora	Rmu_sc0007063.1_g000001
rosa_roxburghii	Rroxscaffold_5G00367790
rosa_rugosa	Rorug04G0206200 Rorug04G0206300
rosa_samantha	Rh4AG263500 Rh4BG269400 Rh4CG281800 Rh4DG265800
rosa_wichuraiana	Rw4G022740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	49
AccBSI	CCGCTC	1 cut(s)	275
AccI	GTMKAC	1 cut(s)	809
AccII	CGCG	1 cut(s)	772
AccIII	TCCGGA	1 cut(s)	481
AciI	CCGC	8 cut(s)	273, 419, 668, 770, 800, 830, 981, 1106
AclWI	GGATC	1 cut(s)	473
AcoI	YGGCCR	1 cut(s)	610
AcsI	RAATTY	1 cut(s)	55
AcuI	CTGAAG	1 cut(s)	1076
AcyI	GRCGYC	1 cut(s)	377
AdeI	CACNNNGTG	1 cut(s)	773
AfaI	GTAC	1 cut(s)	364
AfiI	CCNNNNNNNGG	1 cut(s)	49
AgsI	TTSAA	5 cut(s)	355, 505, 943, 1022, 1040
AhdI	GACNNNNNGTC	1 cut(s)	712
AhlI	ACTAGT	1 cut(s)	1010
AjnI	CCWGG	1 cut(s)	641
AluBI	AGCT	4 cut(s)	416, 620, 648, 951
AluI	AGCT	4 cut(s)	416, 620, 648, 951
Alw26I	GTCTC	1 cut(s)	1071
AlwI	GGATC	1 cut(s)	473
Aor13HI	TCCGGA	1 cut(s)	481
AoxI	GGCC	3 cut(s)	436, 610, 739
ApeKI	GCWGC	3 cut(s)	416, 827, 898
ApoI	RAATTY	1 cut(s)	55
AspS9I	GGNCC	3 cut(s)	436, 983, 1043
AsuHPI	GGTGA	2 cut(s)	239, 718
AsuII	TTCGAA	1 cut(s)	1114
AvaII	GGWCC	2 cut(s)	983, 1043
BanII	GRGCYC	1 cut(s)	1125
BbsI	GAAGAC	1 cut(s)	381
BbvI	GCAGC	3 cut(s)	403, 814, 910
BccI	CCATC	3 cut(s)	130, 581, 591
BceAI	ACGGC	2 cut(s)	381, 625
BciT130I	CCWGG	1 cut(s)	643
BcoDI	GTCTC	1 cut(s)	1071
BcuI	ACTAGT	1 cut(s)	1010
BfaI	CTAG	2 cut(s)	581, 1011
BfmI	CTRYAG	1 cut(s)	291
BisI	GCNGC	6 cut(s)	417, 420, 800, 828, 899, 1106
BlsI	GCNGC	6 cut(s)	418, 421, 801, 829, 900, 1107
Bme1390I	CCNGG	1 cut(s)	643
Bme18I	GGWCC	2 cut(s)	983, 1043
BmeRI	GACNNNNNGTC	1 cut(s)	712
BmgT120I	GGNCC	3 cut(s)	436, 983, 1043
BmiI	GGNNCC	4 cut(s)	369, 540, 1045, 1122
BmrFI	CCNGG	1 cut(s)	643
BmrI	ACTGGG	1 cut(s)	822
BmsI	GCATC	6 cut(s)	228, 290, 294, 760, 811, 845
BmuI	ACTGGG	1 cut(s)	822
BpiI	GAAGAC	1 cut(s)	381
BpmI	CTGGAG	1 cut(s)	261
Bpu14I	TTCGAA	1 cut(s)	1114
BsaBI	GATNNNNATC	1 cut(s)	1140
BsaHI	GRCGYC	1 cut(s)	377
BsaI	GGTCTC	1 cut(s)	1071
BsaJI	CCNNGG	3 cut(s)	342, 641, 1081
BsaWI	WCCGGW	1 cut(s)	481
BsaXI	ACNNNNNCTCC	2 cut(s)	686, 716
Bsc4I	CCNNNNNNNGG	1 cut(s)	49
Bse1I	ACTGG	1 cut(s)	817
Bse8I	GATNNNNATC	1 cut(s)	1140
BseAI	TCCGGA	1 cut(s)	481
BseBI	CCWGG	1 cut(s)	643
BseDI	CCNNGG	3 cut(s)	342, 641, 1081
BseGI	GGATG	2 cut(s)	10, 751
BseJI	GATNNNNATC	1 cut(s)	1140
BseLI	CCNNNNNNNGG	1 cut(s)	49
BseNI	ACTGG	1 cut(s)	817
BseRI	GAGGAG	1 cut(s)	988
BseXI	GCAGC	3 cut(s)	403, 814, 910
BsgI	GTGCAG	1 cut(s)	473
Bsh1236I	CGCG	1 cut(s)	772
Bsh1285I	CGRYCG	1 cut(s)	37
BshFI	GGCC	3 cut(s)	438, 612, 741
BsiEI	CGRYCG	1 cut(s)	37
BsiSI	CCGG	3 cut(s)	105, 308, 482
BslFI	GGGAC	1 cut(s)	744
BslI	CCNNNNNNNGG	1 cut(s)	49
BsmAI	GTCTC	1 cut(s)	1071
BsmFI	GGGAC	1 cut(s)	744
BsnI	GGCC	3 cut(s)	438, 612, 741
Bso31I	GGTCTC	1 cut(s)	1071
Bsp119I	TTCGAA	1 cut(s)	1114
Bsp1286I	GDGCHC	1 cut(s)	1125
Bsp13I	TCCGGA	1 cut(s)	481
Bsp143I	GATC	6 cut(s)	34, 136, 263, 393, 478, 1135
Bsp19I	CCATGG	1 cut(s)	1081
BspACI	CCGC	8 cut(s)	273, 419, 668, 770, 800, 830, 981, 1106
BspANI	GGCC	3 cut(s)	438, 612, 741
BspEI	TCCGGA	1 cut(s)	481
BspFNI	CGCG	1 cut(s)	772
BspHI	TCATGA	2 cut(s)	231, 555
BspLI	GGNNCC	4 cut(s)	369, 540, 1045, 1122
BspPI	GGATC	1 cut(s)	473
BspQI	GCTCTTC	1 cut(s)	958
BspT104I	TTCGAA	1 cut(s)	1114
BspTNI	GGTCTC	1 cut(s)	1071
BsrBI	CCGCTC	1 cut(s)	275
BsrI	ACTGG	1 cut(s)	817
BssECI	CCNNGG	3 cut(s)	342, 641, 1081
BssMI	GATC	6 cut(s)	34, 136, 263, 393, 478, 1135
BssNI	GRCGYC	1 cut(s)	377
BssT1I	CCWWGG	1 cut(s)	1081
Bst2UI	CCWGG	1 cut(s)	643
Bst4CI	ACNGT	3 cut(s)	295, 721, 965
Bst6I	CTCTTC	4 cut(s)	403, 453, 680, 958
BstACI	GRCGYC	1 cut(s)	377
BstBI	TTCGAA	1 cut(s)	1114
BstC8I	GCNNGC	1 cut(s)	670
BstDEI	CTNAG	1 cut(s)	1073
BstDSI	CCRYGG	1 cut(s)	1081
BstEII	GGTNACC	1 cut(s)	724
BstF5I	GGATG	2 cut(s)	10, 751
BstFNI	CGCG	1 cut(s)	772
BstKTI	GATC	6 cut(s)	37, 139, 266, 396, 481, 1138
BstMAI	GTCTC	1 cut(s)	1071
BstMBI	GATC	6 cut(s)	34, 136, 263, 393, 478, 1135
BstMCI	CGRYCG	1 cut(s)	37
BstMWI	GCNNNNNNNGC	4 cut(s)	59, 538, 833, 904
BstNI	CCWGG	1 cut(s)	643
BstNSI	RCATGY	1 cut(s)	121
BstPI	GGTNACC	1 cut(s)	724
BstSCI	CCNGG	1 cut(s)	641
BstSFI	CTRYAG	1 cut(s)	291
BstUI	CGCG	1 cut(s)	772
BstV1I	GCAGC	3 cut(s)	403, 814, 910
BstV2I	GAAGAC	1 cut(s)	381
BstX2I	RGATCY	1 cut(s)	478
BstYI	RGATCY	1 cut(s)	478
BsuRI	GGCC	3 cut(s)	438, 612, 741
BtgI	CCRYGG	1 cut(s)	1081
BtgZI	GCGATG	1 cut(s)	317
BtsCI	GGATG	2 cut(s)	10, 751
BtsI	GCAGTG	2 cut(s)	663, 794
BtsIMutI	CAGTG	3 cut(s)	663, 717, 794
Cac8I	GCNNGC	1 cut(s)	670
CciI	TCATGA	2 cut(s)	231, 555
Cfr13I	GGNCC	3 cut(s)	436, 983, 1043
CseI	GACGC	1 cut(s)	385
Csp6I	GTAC	1 cut(s)	363
CviAII	CATG	6 cut(s)	49, 118, 163, 232, 556, 1082
CviQI	GTAC	1 cut(s)	363
DdeI	CTNAG	1 cut(s)	1073
DpnI	GATC	6 cut(s)	36, 138, 265, 395, 480, 1137
DpnII	GATC	6 cut(s)	34, 136, 263, 393, 478, 1135
DraIII	CACNNNGTG	1 cut(s)	773
DriI	GACNNNNNGTC	1 cut(s)	712
EaeI	YGGCCR	1 cut(s)	610
Eam1104I	CTCTTC	4 cut(s)	403, 453, 680, 958
Eam1105I	GACNNNNNGTC	1 cut(s)	712
EarI	CTCTTC	4 cut(s)	403, 453, 680, 958
Eco130I	CCWWGG	1 cut(s)	1081
Eco24I	GRGCYC	1 cut(s)	1125
Eco31I	GGTCTC	1 cut(s)	1071
Eco32I	GATATC	1 cut(s)	1099
Eco47I	GGWCC	2 cut(s)	983, 1043
Eco57I	CTGAAG	1 cut(s)	1076
Eco91I	GGTNACC	1 cut(s)	724
EcoO109I	RGGNCCY	1 cut(s)	1043
EcoO65I	GGTNACC	1 cut(s)	724
EcoRII	CCWGG	1 cut(s)	641
EcoRV	GATATC	1 cut(s)	1099
EcoT14I	CCWWGG	1 cut(s)	1081
EcoT38I	GRGCYC	1 cut(s)	1125
ErhI	CCWWGG	1 cut(s)	1081
FaeI	CATG	6 cut(s)	52, 121, 166, 235, 559, 1085
FaqI	GGGAC	1 cut(s)	744
FatI	CATG	6 cut(s)	48, 117, 162, 231, 555, 1081
FauI	CCCGC	2 cut(s)	280, 661
FblI	GTMKAC	1 cut(s)	809
Fnu4HI	GCNGC	6 cut(s)	417, 420, 800, 828, 899, 1106
FokI	GGATG	2 cut(s)	17, 738
FriOI	GRGCYC	1 cut(s)	1125
Fsp4HI	GCNGC	6 cut(s)	417, 420, 800, 828, 899, 1106
FspBI	CTAG	2 cut(s)	581, 1011
GluI	GCNGC	6 cut(s)	417, 420, 800, 828, 899, 1106
GsuI	CTGGAG	1 cut(s)	261
HaeIII	GGCC	3 cut(s)	438, 612, 741
HapII	CCGG	3 cut(s)	105, 308, 482
HgaI	GACGC	1 cut(s)	385
Hin1I	GRCGYC	1 cut(s)	377
Hin1II	CATG	6 cut(s)	52, 121, 166, 235, 559, 1085
HinfI	GANTC	2 cut(s)	79, 1111
HpaII	CCGG	3 cut(s)	105, 308, 482
HphI	GGTGA	2 cut(s)	239, 718
Hpy166II	GTNNAC	3 cut(s)	717, 810, 1016
Hpy188I	TCNGA	6 cut(s)	73, 84, 141, 346, 372, 706
Hpy188III	TCNNGA	5 cut(s)	232, 482, 556, 1022, 1094
Hpy8I	GTNNAC	3 cut(s)	717, 810, 1016
Hpy99I	CGWCG	8 cut(s)	77, 226, 229, 376, 379, 406, 409, 710
HpyAV	CCTTC	7 cut(s)	105, 136, 204, 731, 945, 1034, 1059
HpyCH4III	ACNGT	3 cut(s)	295, 721, 965
HpyCH4IV	ACGT	1 cut(s)	221
HpyCH4V	TGCA	3 cut(s)	454, 660, 791
HpyF10VI	GCNNNNNNNGC	4 cut(s)	59, 538, 833, 904
HpyF3I	CTNAG	1 cut(s)	1073
HpySE526I	ACGT	1 cut(s)	221
Hsp92I	GRCGYC	1 cut(s)	377
Hsp92II	CATG	6 cut(s)	52, 121, 166, 235, 559, 1085
Kpn2I	TCCGGA	1 cut(s)	481
Kzo9I	GATC	6 cut(s)	34, 136, 263, 393, 478, 1135
LguI	GCTCTTC	1 cut(s)	958
LmnI	GCTCC	6 cut(s)	196, 280, 373, 538, 645, 1120
Lsp1109I	GCAGC	3 cut(s)	403, 814, 910
LweI	GCATC	6 cut(s)	228, 290, 294, 760, 811, 845
MaeI	CTAG	2 cut(s)	581, 1011
MaeII	ACGT	1 cut(s)	221
MaeIII	GTNAC	1 cut(s)	724
MalI	GATC	6 cut(s)	36, 138, 265, 395, 480, 1137
MbiI	CCGCTC	1 cut(s)	275
MboI	GATC	6 cut(s)	34, 136, 263, 393, 478, 1135
MfeI	CAATTG	1 cut(s)	500
MflI	RGATCY	1 cut(s)	478
MhlI	GDGCHC	1 cut(s)	1125
MluCI	AATT	5 cut(s)	55, 500, 786, 1033, 1066
MmeI	TCCRAC	4 cut(s)	96, 107, 395, 729
MroI	TCCGGA	1 cut(s)	481
MseI	TTAA	1 cut(s)	990
MslI	CAYNNNNRTG	2 cut(s)	127, 236
MspI	CCGG	3 cut(s)	105, 308, 482
MspR9I	CCNGG	1 cut(s)	643
MunI	CAATTG	1 cut(s)	500
MvaI	CCWGG	1 cut(s)	643
MvnI	CGCG	1 cut(s)	772
MwoI	GCNNNNNNNGC	4 cut(s)	59, 538, 833, 904
NcoI	CCATGG	1 cut(s)	1081
NdeII	GATC	6 cut(s)	34, 136, 263, 393, 478, 1135
NlaIII	CATG	6 cut(s)	52, 121, 166, 235, 559, 1085
NlaIV	GGNNCC	4 cut(s)	369, 540, 1045, 1122
NmeAIII	GCCGAG	1 cut(s)	567
NmuCI	GTSAC	1 cut(s)	724
NspI	RCATGY	1 cut(s)	121
NspV	TTCGAA	1 cut(s)	1114
PagI	TCATGA	2 cut(s)	231, 555
PciSI	GCTCTTC	1 cut(s)	958
PcsI	WCGNNNNNNNCGW	3 cut(s)	371, 383, 398
PfeI	GAWTC	2 cut(s)	79, 1111
PflMI	CCANNNNNTGG	1 cut(s)	49
PkrI	GCNGC	6 cut(s)	418, 421, 801, 829, 900, 1107
Ple19I	CGATCG	1 cut(s)	37
PpuMI	RGGWCCY	1 cut(s)	1043
Psp5II	RGGWCCY	1 cut(s)	1043
Psp6I	CCWGG	1 cut(s)	641
PspEI	GGTNACC	1 cut(s)	724
PspGI	CCWGG	1 cut(s)	641
PspN4I	GGNNCC	4 cut(s)	369, 540, 1045, 1122
PspPI	GGNCC	3 cut(s)	436, 983, 1043
PspPPI	RGGWCCY	1 cut(s)	1043
PsuI	RGATCY	1 cut(s)	478
PvuI	CGATCG	1 cut(s)	37
RsaI	GTAC	1 cut(s)	364
RsaNI	GTAC	1 cut(s)	363
RseI	CAYNNNNRTG	2 cut(s)	127, 236
SapI	GCTCTTC	1 cut(s)	958
SaqAI	TTAA	1 cut(s)	990
SatI	GCNGC	6 cut(s)	417, 420, 800, 828, 899, 1106
Sau3AI	GATC	6 cut(s)	34, 136, 263, 393, 478, 1135
Sau96I	GGNCC	3 cut(s)	436, 983, 1043
ScrFI	CCNGG	1 cut(s)	643
SduI	GDGCHC	1 cut(s)	1125
SfaNI	GCATC	6 cut(s)	228, 290, 294, 760, 811, 845
SfcI	CTRYAG	1 cut(s)	291
SfuI	TTCGAA	1 cut(s)	1114
SinI	GGWCC	2 cut(s)	983, 1043
SmiMI	CAYNNNNRTG	2 cut(s)	127, 236
SpeI	ACTAGT	1 cut(s)	1010
Sse9I	AATT	5 cut(s)	55, 500, 786, 1033, 1066
SsiI	CCGC	8 cut(s)	273, 419, 668, 770, 800, 830, 981, 1106
SspMI	CTAG	2 cut(s)	581, 1011
StyD4I	CCNGG	1 cut(s)	641
StyI	CCWWGG	1 cut(s)	1081
TaaI	ACNGT	3 cut(s)	295, 721, 965
TaiI	ACGT	1 cut(s)	224
TaqI	TCGA	5 cut(s)	114, 204, 392, 407, 1114
TasI	AATT	5 cut(s)	55, 500, 786, 1033, 1066
TauI	GCSGC	3 cut(s)	422, 802, 1108
TfiI	GAWTC	2 cut(s)	79, 1111
Tru1I	TTAA	1 cut(s)	990
Tru9I	TTAA	1 cut(s)	990
TscAI	CASTG	3 cut(s)	670, 724, 801
TseFI	GTSAC	1 cut(s)	724
TseI	GCWGC	3 cut(s)	416, 827, 898
Tsp45I	GTSAC	1 cut(s)	724
TspDTI	ATGAA	4 cut(s)	151, 426, 458, 544
TspRI	CASTG	3 cut(s)	670, 724, 801
Van91I	CCANNNNNTGG	1 cut(s)	49
VpaK11BI	GGWCC	2 cut(s)	983, 1043
XapI	RAATTY	1 cut(s)	55
XceI	RCATGY	1 cut(s)	121
XmiI	GTMKAC	1 cut(s)	809
XspI	CTAG	2 cut(s)	581, 1011

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.