Rh4CG281800
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0014718
Physical Location & Seq
Forward (+)
53957884 .. 53959400
1517 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG281800.1

Sequence Viewer

Length: 1167 bp
ATGGATGGTAGACAGGAGCTGAGAAACAACACCACCACCACCAATCGTACTACTCTTCCTCCTTCCTCCTCCTCCTCCTCTGCACCGATGGCCAACTCAGCACTTTTCTCCGATCACGAGATTCCGATGATACCCAACAGCACCTTCCCTTCACTCTCAAGCATCTTCGACTCAGAACCCGAACGGGGTTATCTGGGTTTCATGGAGTTGCTCGGCAACACGGACTTCGCTCCTGTGGTTGATTTCGGGACCCAAACGACGCCGTCTGACTCGTTGGTGACGATGATGATGCCACCTCAGCAGCAAACACAAACACAAGCGCTTCCGTCGCCGGTGTCGTCTACAGTGCCAGAGAGTGGGACTAACTCCGAGGCCTTGAACTCCAGCGCCAATACTGCTCCCACGACACCCAACTCGTCTTCGATCTCGTCCTCGTCTAATGAAGCTCATGCGGCGAATAATAATAATGAGGAGCAAACCACGACAAAAGCAGGAGATGAGGAAGAAGATGCTGACCAAGATCCGGACAAGACCCAGAAACAATTGAAACCCAAAAAGAAGAACCAGAAAAGGCAGAGGGAGCCGAGATTTGCGTTCATGACCAAGAGCGAGGTCGATAACCTAGACGACGGGTACAGATGGCGCAAGTACGGCCAAAAAGCTGTCAAGAACAGCCCTTATCCAAGGAGCTACTATCGTTGCACCACAGCAGGTTGCGGTGTGAAGAAGAGGGTGGAGAGGTCCTCCGACGACCCTTCGATTGTGGTGACGACTTACGAAGGCCAGCACACGCACCCGAGCCCGATCACGCCGCGCGGCAGCATGGGGATGGCGCCGCTGCCGTCGGAGCTGTCCGGTCATTTGTTCACTGGGGCAGGCTCAGGCTATGGTGTTCCTCATCACTATCAACATCAGCAGCAACAGCAACAGCATTTGCACTCTTATATATATAACTCTTCGCCTTCTTTGAACATTACTACTACCACCTCCTCAGCGCCATCCTATGGTTTCAACCCGGCGTCGTTTCCTGGTTTGTTTCAAGAGAGATATAATAACACTGGAGGAGGTCATTCCTCTAATTTGGCCGCTTCTAATTTGCTTAGAGACCACGGACTTCTTCAGGATATGCTGCCATCGCAGATTCGAAAAGAGACTAAGGAAGGGTAG

Protein Analysis

388

Amino Acids

42.38

Weight (kDa)

6.06

Isoelectric Point (pI)

56.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 209 - 266 2e-28 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014718)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g20230
malus_domestica MD13G1150700.v1.1 MD16G1151000.v1.1
prunus_persica Prupe.1G114800_v2.0.a1
pyrus_communis pycom13g12950 pycom16g12920
rosa_chinensis RchiOBHm_Chr4g0425801
rosa_laevigata RLG00000007339
rosa_multiflora Rmu_sc0007063.1_g000001
rosa_roxburghii Rroxscaffold_5G00367790
rosa_rugosa Rorug04G0206200 Rorug04G0206300
rosa_samantha Rh4AG263500 Rh4BG269400 Rh4CG281800 Rh4DG265800
rosa_wichuraiana Rw4G022740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 701
AccB1I GGYRCC 1 cut(s) 832
AccB7I CCANNNNNTGG 2 cut(s) 356, 1004
AccI GTMKAC 2 cut(s) 10, 341
AccII CGCG 2 cut(s) 814, 816
AccIII TCCGGA 1 cut(s) 523
AciI CCGC 6 cut(s) 452, 717, 812, 816, 836, 1086
AclWI GGATC 1 cut(s) 515
AcoI YGGCCR 3 cut(s) 90, 652, 1083
AcuI CTGAAG 1 cut(s) 1103
AcyI GRCGYC 3 cut(s) 260, 833, 1019
AfaI GTAC 3 cut(s) 49, 635, 650
AfeI AGCGCT 1 cut(s) 321
AfiI CCNNNNNNNGG 4 cut(s) 185, 356, 523, 1004
AgsI TTSAA 5 cut(s) 379, 547, 970, 1012, 1040
AjnI CCWGG 1 cut(s) 1027
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
AluBI AGCT 5 cut(s) 19, 446, 662, 690, 850
AluI AGCT 5 cut(s) 19, 446, 662, 690, 850
Alw26I GTCTC 2 cut(s) 1098, 1145
AlwI GGATC 1 cut(s) 515
AlwNI CAGNNNCTG 1 cut(s) 19
Ama87I CYCGRG 1 cut(s) 796
Aor13HI TCCGGA 1 cut(s) 523
Aor51HI AGCGCT 1 cut(s) 321
AoxI GGCC 5 cut(s) 90, 372, 652, 781, 1083
ApeKI GCWGC 5 cut(s) 301, 819, 838, 916, 1129
AspLEI GCGC 6 cut(s) 322, 389, 645, 816, 835, 997
AspS9I GGNCC 2 cut(s) 249, 741
AsuC2I CCSGG 1 cut(s) 1016
AsuHPI GGTGA 2 cut(s) 289, 778
AsuII TTCGAA 1 cut(s) 1144
AvaI CYCGRG 1 cut(s) 796
AvaII GGWCC 2 cut(s) 249, 741
BalI TGGCCA 1 cut(s) 92
BanI GGYRCC 1 cut(s) 832
BanII GRGCYC 1 cut(s) 803
BauI CACGAG 1 cut(s) 116
BbsI GAAGAC 1 cut(s) 411
BbvCI CCTCAGC 2 cut(s) 297, 991
BbvI GCAGC 5 cut(s) 313, 825, 831, 928, 1116
BccI CCATC 5 cut(s) 82, 633, 823, 1006, 1141
BceAI ACGGC 3 cut(s) 247, 667, 826
BcgI CGANNNNNNTGC 2 cut(s) 271, 305
BciT130I CCWGG 1 cut(s) 1029
BcnI CCSGG 1 cut(s) 1016
BcoDI GTCTC 2 cut(s) 1098, 1145
BfaI CTAG 1 cut(s) 623
BfmI CTRYAG 1 cut(s) 342
BfoI RGCGCY 4 cut(s) 323, 390, 836, 998
BfuAI ACCTGC 1 cut(s) 701
Bme1390I CCNGG 2 cut(s) 1016, 1029
Bme18I GGWCC 2 cut(s) 249, 741
BmeT110I CYCGRG 1 cut(s) 796
BmgT120I GGNCC 2 cut(s) 249, 741
BmiI GGNNCC 4 cut(s) 250, 251, 582, 834
BmrFI CCNGG 2 cut(s) 1016, 1029
BmrI ACTGGG 1 cut(s) 879
BmsI GCATC 3 cut(s) 171, 279, 499
BmuI ACTGGG 1 cut(s) 879
BpiI GAAGAC 1 cut(s) 411
BplI GAGNNNNNCTC 2 cut(s) 728, 760
BpmI CTGGAG 2 cut(s) 367, 1080
Bpu10I CCTNAGC 3 cut(s) 297, 880, 991
Bpu14I TTCGAA 1 cut(s) 1144
BpuEI CTTGAG 1 cut(s) 142
BpuMI CCSGG 1 cut(s) 1016
BsaHI GRCGYC 3 cut(s) 260, 833, 1019
BsaI GGTCTC 1 cut(s) 1098
BsaJI CCNNGG 3 cut(s) 369, 683, 1108
BsaWI WCCGGW 2 cut(s) 523, 854
BsaXI ACNNNNNCTCC 2 cut(s) 43, 73
Bsc4I CCNNNNNNNGG 4 cut(s) 185, 356, 523, 1004
Bse118I RCCGGY 1 cut(s) 331
Bse1I ACTGG 2 cut(s) 874, 1063
BseAI TCCGGA 1 cut(s) 523
BseBI CCWGG 1 cut(s) 1029
BseDI CCNNGG 3 cut(s) 369, 683, 1108
BseGI GGATG 3 cut(s) 10, 834, 998
BseLI CCNNNNNNNGG 4 cut(s) 185, 356, 523, 1004
BseMII CTCAG 6 cut(s) 11, 111, 186, 311, 894, 1005
BseNI ACTGG 2 cut(s) 874, 1063
BseRI GAGGAG 7 cut(s) 58, 61, 64, 67, 485, 979, 1077
BseXI GCAGC 5 cut(s) 313, 825, 831, 928, 1116
BsgI GTGCAG 1 cut(s) 66
Bsh1236I CGCG 2 cut(s) 814, 816
BshFI GGCC 5 cut(s) 92, 374, 654, 783, 1085
BshNI GGYRCC 1 cut(s) 832
BsiHKCI CYCGRG 1 cut(s) 796
BsiSI CCGG 4 cut(s) 332, 524, 855, 1016
BslFI GGGAC 2 cut(s) 262, 373
BslI CCNNNNNNNGG 4 cut(s) 185, 356, 523, 1004
BsmAI GTCTC 2 cut(s) 1098, 1145
BsmFI GGGAC 2 cut(s) 262, 373
BsnI GGCC 5 cut(s) 92, 374, 654, 783, 1085
Bso31I GGTCTC 1 cut(s) 1098
BsoBI CYCGRG 1 cut(s) 796
Bsp119I TTCGAA 1 cut(s) 1144
Bsp1286I GDGCHC 1 cut(s) 803
Bsp13I TCCGGA 1 cut(s) 523
Bsp143I GATC 4 cut(s) 112, 423, 520, 804
BspACI CCGC 6 cut(s) 452, 717, 812, 816, 836, 1086
BspANI GGCC 5 cut(s) 92, 374, 654, 783, 1085
BspCNI CTCAG 6 cut(s) 12, 110, 185, 310, 893, 1004
BspEI TCCGGA 1 cut(s) 523
BspFNI CGCG 2 cut(s) 814, 816
BspHI TCATGA 1 cut(s) 597
BspLI GGNNCC 4 cut(s) 250, 251, 582, 834
BspMI ACCTGC 1 cut(s) 701
BspPI GGATC 1 cut(s) 515
BspT104I TTCGAA 1 cut(s) 1144
BspT107I GGYRCC 1 cut(s) 832
BspTNI GGTCTC 1 cut(s) 1098
BsrFI RCCGGY 1 cut(s) 331
BsrI ACTGG 2 cut(s) 874, 1063
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 3 cut(s) 369, 683, 1108
BssMI GATC 4 cut(s) 112, 423, 520, 804
BssNI GRCGYC 3 cut(s) 260, 833, 1019
BssSI CACGAG 1 cut(s) 116
BssT1I CCWWGG 1 cut(s) 683
Bst2BI CACGAG 1 cut(s) 116
Bst2UI CCWGG 1 cut(s) 1029
Bst4CI ACNGT 1 cut(s) 346
Bst6I CTCTTC 3 cut(s) 60, 722, 961
BstACI GRCGYC 3 cut(s) 260, 833, 1019
BstBI TTCGAA 1 cut(s) 1144
BstC8I GCNNGC 2 cut(s) 785, 877
BstDEI CTNAG 8 cut(s) 20, 97, 172, 297, 880, 991, 1100, 1155
BstDSI CCRYGG 1 cut(s) 1108
BstF5I GGATG 3 cut(s) 10, 834, 998
BstFNI CGCG 2 cut(s) 814, 816
BstH2I RGCGCY 4 cut(s) 323, 390, 836, 998
BstHHI GCGC 6 cut(s) 322, 389, 645, 816, 835, 997
BstKTI GATC 4 cut(s) 115, 426, 523, 807
BstMAI GTCTC 2 cut(s) 1098, 1145
BstMBI GATC 4 cut(s) 112, 423, 520, 804
BstNI CCWGG 1 cut(s) 1029
BstSCI CCNGG 2 cut(s) 1014, 1027
BstSFI CTRYAG 1 cut(s) 342
BstUI CGCG 2 cut(s) 814, 816
BstV1I GCAGC 5 cut(s) 313, 825, 831, 928, 1116
BstV2I GAAGAC 1 cut(s) 411
BstX2I RGATCY 1 cut(s) 520
BstYI RGATCY 1 cut(s) 520
BsuRI GGCC 5 cut(s) 92, 374, 654, 783, 1085
BtgI CCRYGG 1 cut(s) 1108
BtgZI GCGATG 1 cut(s) 1119
BtsCI GGATG 3 cut(s) 10, 834, 998
BtsIMutI CAGTG 3 cut(s) 351, 867, 1056
BveI ACCTGC 1 cut(s) 701
Cac8I GCNNGC 2 cut(s) 785, 877
CaiI CAGNNNCTG 1 cut(s) 19
CciI TCATGA 1 cut(s) 597
CfoI GCGC 6 cut(s) 322, 389, 645, 816, 835, 997
Cfr10I RCCGGY 1 cut(s) 331
Cfr13I GGNCC 2 cut(s) 249, 741
CseI GACGC 2 cut(s) 268, 1008
Csp6I GTAC 3 cut(s) 48, 634, 649
CviAII CATG 4 cut(s) 202, 449, 598, 823
CviQI GTAC 3 cut(s) 48, 634, 649
DdeI CTNAG 8 cut(s) 20, 97, 172, 297, 880, 991, 1100, 1155
DinI GGCGCC 1 cut(s) 834
DpnI GATC 4 cut(s) 114, 425, 522, 806
DpnII GATC 4 cut(s) 112, 423, 520, 804
EaeI YGGCCR 3 cut(s) 90, 652, 1083
Eam1104I CTCTTC 3 cut(s) 60, 722, 961
EarI CTCTTC 3 cut(s) 60, 722, 961
Eco130I CCWWGG 1 cut(s) 683
Eco147I AGGCCT 1 cut(s) 374
Eco24I GRGCYC 1 cut(s) 803
Eco31I GGTCTC 1 cut(s) 1098
Eco47I GGWCC 2 cut(s) 249, 741
Eco47III AGCGCT 1 cut(s) 321
Eco57I CTGAAG 1 cut(s) 1103
Eco88I CYCGRG 1 cut(s) 796
EcoO109I RGGNCCY 2 cut(s) 249, 741
EcoRII CCWGG 1 cut(s) 1027
EcoT14I CCWWGG 1 cut(s) 683
EcoT38I GRGCYC 1 cut(s) 803
EgeI GGCGCC 1 cut(s) 834
EheI GGCGCC 1 cut(s) 834
ErhI CCWWGG 1 cut(s) 683
FaeI CATG 4 cut(s) 205, 452, 601, 826
FaqI GGGAC 2 cut(s) 262, 373
FatI CATG 4 cut(s) 201, 448, 597, 822
FblI GTMKAC 2 cut(s) 10, 341
FokI GGATG 3 cut(s) 17, 841, 985
FriOI GRGCYC 1 cut(s) 803
FspBI CTAG 1 cut(s) 623
GlaI GCGC 6 cut(s) 321, 388, 644, 815, 834, 996
GsuI CTGGAG 2 cut(s) 367, 1080
HaeII RGCGCY 4 cut(s) 323, 390, 836, 998
HaeIII GGCC 5 cut(s) 92, 374, 654, 783, 1085
HapII CCGG 4 cut(s) 332, 524, 855, 1016
HgaI GACGC 2 cut(s) 268, 1008
HhaI GCGC 6 cut(s) 322, 389, 645, 816, 835, 997
Hin1I GRCGYC 3 cut(s) 260, 833, 1019
Hin1II CATG 4 cut(s) 205, 452, 601, 826
Hin6I GCGC 6 cut(s) 320, 387, 643, 814, 833, 995
HinP1I GCGC 6 cut(s) 320, 387, 643, 814, 833, 995
HinfI GANTC 4 cut(s) 121, 170, 269, 1141
HpaII CCGG 4 cut(s) 332, 524, 855, 1016
HphI GGTGA 2 cut(s) 289, 778
Hpy166II GTNNAC 3 cut(s) 11, 342, 867
Hpy188I TCNGA 7 cut(s) 112, 126, 175, 268, 370, 748, 847
Hpy188III TCNNGA 7 cut(s) 116, 247, 524, 598, 667, 1040, 1121
Hpy8I GTNNAC 3 cut(s) 11, 342, 867
Hpy99I CGWCG 6 cut(s) 262, 331, 632, 752, 847, 1024
HpyAV CCTTC 7 cut(s) 72, 154, 159, 765, 773, 972, 1154
HpyCH4III ACNGT 1 cut(s) 346
HpyCH4V TGCA 3 cut(s) 83, 702, 937
HpyF3I CTNAG 8 cut(s) 20, 97, 172, 297, 880, 991, 1100, 1155
Hsp92I GRCGYC 3 cut(s) 260, 833, 1019
Hsp92II CATG 4 cut(s) 205, 452, 601, 826
HspAI GCGC 6 cut(s) 320, 387, 643, 814, 833, 995
KasI GGCGCC 1 cut(s) 832
KflI GGGWCCC 1 cut(s) 249
Kpn2I TCCGGA 1 cut(s) 523
Kzo9I GATC 4 cut(s) 112, 423, 520, 804
LmnI GCTCC 7 cut(s) 16, 235, 403, 472, 580, 687, 847
Lsp1109I GCAGC 5 cut(s) 313, 825, 831, 928, 1116
LweI GCATC 3 cut(s) 171, 279, 499
MaeI CTAG 1 cut(s) 623
MaeIII GTNAC 2 cut(s) 277, 766
MalI GATC 4 cut(s) 114, 425, 522, 806
MboI GATC 4 cut(s) 112, 423, 520, 804
MfeI CAATTG 1 cut(s) 542
MflI RGATCY 1 cut(s) 520
MhlI GDGCHC 1 cut(s) 803
MlsI TGGCCA 1 cut(s) 92
MluCI AATT 3 cut(s) 542, 1078, 1093
MluNI TGGCCA 1 cut(s) 92
Mly113I GGCGCC 1 cut(s) 833
MlyI GAGTC 2 cut(s) 164, 263
MmeI TCCRAC 2 cut(s) 771, 825
Mox20I TGGCCA 1 cut(s) 92
MroI TCCGGA 1 cut(s) 523
MscI TGGCCA 1 cut(s) 92
MslI CAYNNNNRTG 1 cut(s) 827
Msp20I TGGCCA 1 cut(s) 92
MspA1I CMGCKG 1 cut(s) 838
MspI CCGG 4 cut(s) 332, 524, 855, 1016
MspR9I CCNGG 2 cut(s) 1016, 1029
MunI CAATTG 1 cut(s) 542
MvaI CCWGG 1 cut(s) 1029
MvnI CGCG 2 cut(s) 814, 816
NarI GGCGCC 1 cut(s) 833
NciI CCSGG 1 cut(s) 1016
NdeII GATC 4 cut(s) 112, 423, 520, 804
NlaIII CATG 4 cut(s) 205, 452, 601, 826
NlaIV GGNNCC 4 cut(s) 250, 251, 582, 834
NmeAIII GCCGAG 2 cut(s) 192, 609
NmuCI GTSAC 2 cut(s) 277, 766
NspV TTCGAA 1 cut(s) 1144
PagI TCATGA 1 cut(s) 597
PceI AGGCCT 1 cut(s) 374
PcsI WCGNNNNNNNCGW 2 cut(s) 278, 335
PfeI GAWTC 2 cut(s) 121, 1141
PflFI GACNNNGTC 1 cut(s) 262
PflMI CCANNNNNTGG 2 cut(s) 356, 1004
PleI GAGTC 2 cut(s) 164, 263
PluTI GGCGCC 1 cut(s) 836
PpsI GAGTC 2 cut(s) 164, 263
PpuMI RGGWCCY 2 cut(s) 249, 741
Psp5II RGGWCCY 2 cut(s) 249, 741
Psp6I CCWGG 1 cut(s) 1027
PspGI CCWGG 1 cut(s) 1027
PspN4I GGNNCC 4 cut(s) 250, 251, 582, 834
PspPI GGNCC 2 cut(s) 249, 741
PspPPI RGGWCCY 2 cut(s) 249, 741
PstNI CAGNNNCTG 1 cut(s) 19
PsuI RGATCY 1 cut(s) 520
PsyI GACNNNGTC 1 cut(s) 262
RsaI GTAC 3 cut(s) 49, 635, 650
RsaNI GTAC 3 cut(s) 48, 634, 649
RseI CAYNNNNRTG 1 cut(s) 827
Sau3AI GATC 4 cut(s) 112, 423, 520, 804
Sau96I GGNCC 2 cut(s) 249, 741
SchI GAGTC 2 cut(s) 164, 263
ScrFI CCNGG 2 cut(s) 1016, 1029
SduI GDGCHC 1 cut(s) 803
SfaNI GCATC 3 cut(s) 171, 279, 499
SfcI CTRYAG 1 cut(s) 342
SfoI GGCGCC 1 cut(s) 834
SfuI TTCGAA 1 cut(s) 1144
SgrAI CRCCGGYG 1 cut(s) 331
SinI GGWCC 2 cut(s) 249, 741
SmiMI CAYNNNNRTG 1 cut(s) 827
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
Sse9I AATT 3 cut(s) 542, 1078, 1093
SseBI AGGCCT 1 cut(s) 374
SsiI CCGC 6 cut(s) 452, 717, 812, 816, 836, 1086
SspDI GGCGCC 1 cut(s) 832
SspMI CTAG 1 cut(s) 623
StuI AGGCCT 1 cut(s) 374
StyD4I CCNGG 2 cut(s) 1014, 1027
StyI CCWWGG 1 cut(s) 683
TaaI ACNGT 1 cut(s) 346
TaqI TCGA 5 cut(s) 168, 422, 615, 758, 1144
TasI AATT 3 cut(s) 542, 1078, 1093
TauI GCSGC 5 cut(s) 455, 814, 819, 838, 1088
TfiI GAWTC 2 cut(s) 121, 1141
TscAI CASTG 3 cut(s) 351, 874, 1063
TseFI GTSAC 2 cut(s) 277, 766
TseI GCWGC 5 cut(s) 301, 819, 838, 916, 1129
Tsp45I GTSAC 2 cut(s) 277, 766
TspDTI ATGAA 3 cut(s) 190, 456, 586
TspGWI ACGGA 3 cut(s) 236, 315, 1125
TspRI CASTG 3 cut(s) 351, 874, 1063
Tth111I GACNNNGTC 1 cut(s) 262
Van91I CCANNNNNTGG 2 cut(s) 356, 1004
VpaK11BI GGWCC 2 cut(s) 249, 741
XmiI GTMKAC 2 cut(s) 10, 341
XspI CTAG 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.