Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr13

Orthogroup ID

OG0014718

Physical Location & Seq

Genomic Coordinates Reverse (-)

9154513 .. 9156319

Gene Structure 1807 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom13g12950.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1218 bp

ATGGATGGTAGGGAAGAAAAGCTAAAGACCACCGATCGTAGCAACAGCATGGCAAATTCGGCAGTTTTCTCCGACGAGATTCCGACCAACTTTCCTTCATTTTCCGGCATATTCGACCTGCCATCTTCGTGCGAAGATCAAAAGGGTTCTCTTGGAGGGTTCATGGACTTGCTCGGAATCCCAGACTACGCTTCTTCTCTATTCGATTTCCCTCAAACTACGTCGTCGCTCATGATGATGCCACCTCACCACAATCAACAACAACCACTTCCCTCTCCAGCATCTACTTCTACAGTAGTAGCGCTGCCGGAGAGGGAGAGTGCTACTACTACCAATACAACCTCGGAGGTTTTGAACAGCGGTACGGCTCCGACAACGCCGAACTCGTCTTCGATCTCTTCGTCGTCGAATGAAGCTGCGGCGAATAATAATGAGGGCCAAATGACAAAAGTGCAGGAAGAGGAAGCCGATGAGCAAGATCCAGAGAAAACCCAGAAACAATTGAAACCGAAAAAGAAGAACCAAAAAAGGCAGAGGGAGCCGAGATTTGCGTTCAAGACAAAGAGTGAGGTTGATAACCTAGATGATGGCTACCGATGGCGAAAATACGGCCAAAAAGCTGTCAAAAATAGCCCTTATCCCAGGAGCTATTATCGTTGCACCACTGCGGGCTGTGGTGTGAAGAAGAGGGTGGAGAGGTCGTCCGACGACCCGTCCACTGTGGTCACCACATACGAAGGCCAACACACGCATCCAAGTCCCATTACACCGCGTGGCACAATGGGAATTGCACCACTGCCGCATCAGTCTACTGGGTTTATATCTGCTGCGGAGGCGTCATCAAATCCCTTTGGTGTTCAGCAGTTTTTACCTCATCATCATCACTATCAACAACAGCAGCAACAGCAATATGCTTATATGTATCAATCCTCCCCTTCTTTGAATATCAGCTCTTCCACCTACGGTGGTGGTGCTGGTGGCGGTCCTTTTAATCCCTCCTCATTTTCTACTAGTTTACTTCAAGAGAGATATAATTTTGAAGGACCCCCTTCTTCTTCTTCGGCTACCAATTTTCTTAGAGACCATGGACTTCTTCAGGATATCGTGCCGCAGATTCGAAAGGAGCCCAAAGAAGAAGATCGATCATCATCAACATCAATAGAATGGATCATAATATTGATTATTGTTTTATGTGTTAATTGCTGCCATGGTATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009891 GO:0009893 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

406

Amino Acids

44.57

Weight (kDa)

6.02

Isoelectric Point (pI)

63.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	195 - 252	6e-28	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014718)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g20230
malus_domestica	MD13G1150700.v1.1 MD16G1151000.v1.1
prunus_persica	Prupe.1G114800_v2.0.a1
pyrus_communis	pycom13g12950 pycom16g12920
rosa_chinensis	RchiOBHm_Chr4g0425801
rosa_laevigata	RLG00000007339
rosa_multiflora	Rmu_sc0007063.1_g000001
rosa_roxburghii	Rroxscaffold_5G00367790
rosa_rugosa	Rorug04G0206200 Rorug04G0206300
rosa_samantha	Rh4AG263500 Rh4BG269400 Rh4CG281800 Rh4DG265800
rosa_wichuraiana	Rw4G022740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	126
AccI	GTMKAC	1 cut(s)	809
AccII	CGCG	1 cut(s)	772
AciI	CCGC	8 cut(s)	360, 419, 668, 770, 800, 830, 981, 1109
AclWI	GGATC	2 cut(s)	473, 1175
AcoI	YGGCCR	1 cut(s)	610
AcsI	RAATTY	1 cut(s)	55
AcuI	CTGAAG	1 cut(s)	1079
AcyI	GRCGYC	1 cut(s)	836
AdeI	CACNNNGTG	1 cut(s)	773
AfaI	GTAC	1 cut(s)	364
AfeI	AGCGCT	1 cut(s)	303
AgsI	TTSAA	6 cut(s)	355, 505, 556, 943, 1022, 1040
AhdI	GACNNNNNGTC	1 cut(s)	712
AhlI	ACTAGT	1 cut(s)	1010
AjnI	CCWGG	1 cut(s)	641
AluBI	AGCT	5 cut(s)	22, 416, 620, 648, 951
AluI	AGCT	5 cut(s)	22, 416, 620, 648, 951
Alw26I	GTCTC	1 cut(s)	1074
AlwI	GGATC	2 cut(s)	473, 1175
Aor51HI	AGCGCT	1 cut(s)	303
AoxI	GGCC	3 cut(s)	436, 610, 739
ApeKI	GCWGC	5 cut(s)	304, 416, 827, 898, 1203
ApoI	RAATTY	1 cut(s)	55
AspLEI	GCGC	1 cut(s)	304
AspS9I	GGNCC	3 cut(s)	436, 983, 1043
AsuHPI	GGTGA	2 cut(s)	239, 718
AsuII	TTCGAA	1 cut(s)	1117
AvaII	GGWCC	2 cut(s)	983, 1043
BanII	GRGCYC	1 cut(s)	1128
BbsI	GAAGAC	1 cut(s)	381
BbvI	GCAGC	5 cut(s)	291, 403, 814, 910, 1190
BccI	CCATC	3 cut(s)	130, 581, 591
BceAI	ACGGC	2 cut(s)	381, 625
BciT130I	CCWGG	1 cut(s)	643
BcoDI	GTCTC	1 cut(s)	1074
BcuI	ACTAGT	1 cut(s)	1010
BfaI	CTAG	2 cut(s)	581, 1011
BfmI	CTRYAG	1 cut(s)	291
BfoI	RGCGCY	1 cut(s)	305
BfuAI	ACCTGC	1 cut(s)	126
BisI	GCNGC	8 cut(s)	305, 417, 420, 800, 828, 899, 1109, 1204
BlsI	GCNGC	8 cut(s)	306, 418, 421, 801, 829, 900, 1110, 1205
Bme1390I	CCNGG	1 cut(s)	643
Bme18I	GGWCC	2 cut(s)	983, 1043
BmeRI	GACNNNNNGTC	1 cut(s)	712
BmgT120I	GGNCC	3 cut(s)	436, 983, 1043
BmiI	GGNNCC	4 cut(s)	369, 540, 1045, 1125
BmrFI	CCNGG	1 cut(s)	643
BmrI	ACTGGG	1 cut(s)	822
BmsI	GCATC	4 cut(s)	228, 290, 760, 811
BmuI	ACTGGG	1 cut(s)	822
BpiI	GAAGAC	1 cut(s)	381
BpmI	CTGGAG	1 cut(s)	261
Bpu14I	TTCGAA	1 cut(s)	1117
Bsa29I	ATCGAT	1 cut(s)	1141
BsaBI	GATNNNNATC	1 cut(s)	1147
BsaHI	GRCGYC	1 cut(s)	836
BsaI	GGTCTC	1 cut(s)	1074
BsaJI	CCNNGG	4 cut(s)	342, 641, 1084, 1207
BsaXI	ACNNNNNCTCC	2 cut(s)	686, 716
Bse1I	ACTGG	1 cut(s)	817
Bse8I	GATNNNNATC	1 cut(s)	1147
BseBI	CCWGG	1 cut(s)	643
BseCI	ATCGAT	1 cut(s)	1141
BseDI	CCNNGG	4 cut(s)	342, 641, 1084, 1207
BseGI	GGATG	2 cut(s)	10, 751
BseJI	GATNNNNATC	1 cut(s)	1147
BseNI	ACTGG	1 cut(s)	817
BseRI	GAGGAG	1 cut(s)	988
BseXI	GCAGC	5 cut(s)	291, 403, 814, 910, 1190
BsgI	GTGCAG	1 cut(s)	473
Bsh1236I	CGCG	1 cut(s)	772
Bsh1285I	CGRYCG	1 cut(s)	37
BshFI	GGCC	3 cut(s)	438, 612, 741
BshVI	ATCGAT	1 cut(s)	1141
BsiEI	CGRYCG	1 cut(s)	37
BsiSI	CCGG	2 cut(s)	105, 308
BslFI	GGGAC	1 cut(s)	744
BsmAI	GTCTC	1 cut(s)	1074
BsmFI	GGGAC	1 cut(s)	744
BsnI	GGCC	3 cut(s)	438, 612, 741
Bso31I	GGTCTC	1 cut(s)	1074
Bsp119I	TTCGAA	1 cut(s)	1117
Bsp1286I	GDGCHC	1 cut(s)	1128
Bsp143I	GATC	7 cut(s)	34, 136, 393, 478, 1138, 1142, 1167
Bsp19I	CCATGG	2 cut(s)	1084, 1207
BspACI	CCGC	8 cut(s)	360, 419, 668, 770, 800, 830, 981, 1109
BspANI	GGCC	3 cut(s)	438, 612, 741
BspDI	ATCGAT	1 cut(s)	1141
BspFNI	CGCG	1 cut(s)	772
BspHI	TCATGA	1 cut(s)	231
BspLI	GGNNCC	4 cut(s)	369, 540, 1045, 1125
BspMI	ACCTGC	1 cut(s)	126
BspPI	GGATC	2 cut(s)	473, 1175
BspQI	GCTCTTC	1 cut(s)	958
BspT104I	TTCGAA	1 cut(s)	1117
BspTNI	GGTCTC	1 cut(s)	1074
BsrI	ACTGG	1 cut(s)	817
BssECI	CCNNGG	4 cut(s)	342, 641, 1084, 1207
BssMI	GATC	7 cut(s)	34, 136, 393, 478, 1138, 1142, 1167
BssNI	GRCGYC	1 cut(s)	836
BssT1I	CCWWGG	2 cut(s)	1084, 1207
Bst2UI	CCWGG	1 cut(s)	643
Bst4CI	ACNGT	3 cut(s)	295, 721, 965
Bst6I	CTCTTC	4 cut(s)	403, 453, 680, 958
BstACI	GRCGYC	1 cut(s)	836
BstBI	TTCGAA	1 cut(s)	1117
BstC8I	GCNNGC	1 cut(s)	670
BstDEI	CTNAG	1 cut(s)	1076
BstDSI	CCRYGG	2 cut(s)	1084, 1207
BstEII	GGTNACC	1 cut(s)	724
BstF5I	GGATG	2 cut(s)	10, 751
BstFNI	CGCG	1 cut(s)	772
BstH2I	RGCGCY	1 cut(s)	305
BstHHI	GCGC	1 cut(s)	304
BstKTI	GATC	7 cut(s)	37, 139, 396, 481, 1141, 1145, 1170
BstMAI	GTCTC	1 cut(s)	1074
BstMBI	GATC	7 cut(s)	34, 136, 393, 478, 1138, 1142, 1167
BstMCI	CGRYCG	1 cut(s)	37
BstMWI	GCNNNNNNNGC	4 cut(s)	59, 538, 833, 904
BstNI	CCWGG	1 cut(s)	643
BstPI	GGTNACC	1 cut(s)	724
BstSCI	CCNGG	1 cut(s)	641
BstSFI	CTRYAG	1 cut(s)	291
BstUI	CGCG	1 cut(s)	772
BstV1I	GCAGC	5 cut(s)	291, 403, 814, 910, 1190
BstV2I	GAAGAC	1 cut(s)	381
BstX2I	RGATCY	1 cut(s)	478
BstYI	RGATCY	1 cut(s)	478
Bsu15I	ATCGAT	1 cut(s)	1141
BsuRI	GGCC	3 cut(s)	438, 612, 741
BsuTUI	ATCGAT	1 cut(s)	1141
BtgI	CCRYGG	2 cut(s)	1084, 1207
BtsCI	GGATG	2 cut(s)	10, 751
BtsI	GCAGTG	2 cut(s)	663, 794
BtsIMutI	CAGTG	3 cut(s)	663, 717, 794
BveI	ACCTGC	1 cut(s)	126
Cac8I	GCNNGC	1 cut(s)	670
CciI	TCATGA	1 cut(s)	231
CfoI	GCGC	1 cut(s)	304
Cfr13I	GGNCC	3 cut(s)	436, 983, 1043
ClaI	ATCGAT	1 cut(s)	1141
CseI	GACGC	1 cut(s)	825
Csp6I	GTAC	1 cut(s)	363
CviAII	CATG	5 cut(s)	49, 163, 232, 1085, 1208
CviQI	GTAC	1 cut(s)	363
DdeI	CTNAG	1 cut(s)	1076
DpnI	GATC	7 cut(s)	36, 138, 395, 480, 1140, 1144, 1169
DpnII	GATC	7 cut(s)	34, 136, 393, 478, 1138, 1142, 1167
DraIII	CACNNNGTG	1 cut(s)	773
DriI	GACNNNNNGTC	1 cut(s)	712
EaeI	YGGCCR	1 cut(s)	610
Eam1104I	CTCTTC	4 cut(s)	403, 453, 680, 958
Eam1105I	GACNNNNNGTC	1 cut(s)	712
EarI	CTCTTC	4 cut(s)	403, 453, 680, 958
Eco130I	CCWWGG	2 cut(s)	1084, 1207
Eco24I	GRGCYC	1 cut(s)	1128
Eco31I	GGTCTC	1 cut(s)	1074
Eco32I	GATATC	1 cut(s)	1102
Eco47I	GGWCC	2 cut(s)	983, 1043
Eco47III	AGCGCT	1 cut(s)	303
Eco57I	CTGAAG	1 cut(s)	1079
Eco91I	GGTNACC	1 cut(s)	724
EcoO109I	RGGNCCY	1 cut(s)	1043
EcoO65I	GGTNACC	1 cut(s)	724
EcoRII	CCWGG	1 cut(s)	641
EcoRV	GATATC	1 cut(s)	1102
EcoT14I	CCWWGG	2 cut(s)	1084, 1207
EcoT38I	GRGCYC	1 cut(s)	1128
ErhI	CCWWGG	2 cut(s)	1084, 1207
FaeI	CATG	5 cut(s)	52, 166, 235, 1088, 1211
FaqI	GGGAC	1 cut(s)	744
FatI	CATG	5 cut(s)	48, 162, 231, 1084, 1207
FauI	CCCGC	1 cut(s)	661
FblI	GTMKAC	1 cut(s)	809
Fnu4HI	GCNGC	8 cut(s)	305, 417, 420, 800, 828, 899, 1109, 1204
FokI	GGATG	2 cut(s)	17, 738
FriOI	GRGCYC	1 cut(s)	1128
Fsp4HI	GCNGC	8 cut(s)	305, 417, 420, 800, 828, 899, 1109, 1204
FspBI	CTAG	2 cut(s)	581, 1011
GlaI	GCGC	1 cut(s)	303
GluI	GCNGC	8 cut(s)	305, 417, 420, 800, 828, 899, 1109, 1204
GsuI	CTGGAG	1 cut(s)	261
HaeII	RGCGCY	1 cut(s)	305
HaeIII	GGCC	3 cut(s)	438, 612, 741
HapII	CCGG	2 cut(s)	105, 308
HgaI	GACGC	1 cut(s)	825
HhaI	GCGC	1 cut(s)	304
Hin1I	GRCGYC	1 cut(s)	836
Hin1II	CATG	5 cut(s)	52, 166, 235, 1088, 1211
Hin6I	GCGC	1 cut(s)	302
HinP1I	GCGC	1 cut(s)	302
HinfI	GANTC	3 cut(s)	79, 177, 1114
HpaII	CCGG	2 cut(s)	105, 308
HphI	GGTGA	2 cut(s)	239, 718
Hpy166II	GTNNAC	3 cut(s)	717, 810, 1016
Hpy188I	TCNGA	6 cut(s)	73, 84, 176, 346, 372, 706
Hpy188III	TCNNGA	5 cut(s)	232, 482, 556, 1022, 1097
Hpy8I	GTNNAC	3 cut(s)	717, 810, 1016
Hpy99I	CGWCG	6 cut(s)	77, 226, 229, 406, 409, 710
HpyAV	CCTTC	5 cut(s)	105, 731, 945, 1034, 1059
HpyCH4III	ACNGT	3 cut(s)	295, 721, 965
HpyCH4IV	ACGT	1 cut(s)	221
HpyCH4V	TGCA	3 cut(s)	454, 660, 791
HpyF10VI	GCNNNNNNNGC	4 cut(s)	59, 538, 833, 904
HpyF3I	CTNAG	1 cut(s)	1076
HpySE526I	ACGT	1 cut(s)	221
Hsp92I	GRCGYC	1 cut(s)	836
Hsp92II	CATG	5 cut(s)	52, 166, 235, 1088, 1211
HspAI	GCGC	1 cut(s)	302
Kzo9I	GATC	7 cut(s)	34, 136, 393, 478, 1138, 1142, 1167
LguI	GCTCTTC	1 cut(s)	958
LmnI	GCTCC	4 cut(s)	373, 538, 645, 1123
Lsp1109I	GCAGC	5 cut(s)	291, 403, 814, 910, 1190
LweI	GCATC	4 cut(s)	228, 290, 760, 811
MaeI	CTAG	2 cut(s)	581, 1011
MaeII	ACGT	1 cut(s)	221
MaeIII	GTNAC	1 cut(s)	724
MalI	GATC	7 cut(s)	36, 138, 395, 480, 1140, 1144, 1169
MboI	GATC	7 cut(s)	34, 136, 393, 478, 1138, 1142, 1167
MfeI	CAATTG	1 cut(s)	500
MflI	RGATCY	1 cut(s)	478
MhlI	GDGCHC	1 cut(s)	1128
MluCI	AATT	6 cut(s)	55, 500, 786, 1033, 1069, 1198
MmeI	TCCRAC	4 cut(s)	96, 107, 395, 729
MseI	TTAA	3 cut(s)	990, 1197, 1216
MslI	CAYNNNNRTG	2 cut(s)	127, 236
MspA1I	CMGCKG	1 cut(s)	360
MspI	CCGG	2 cut(s)	105, 308
MspR9I	CCNGG	1 cut(s)	643
MunI	CAATTG	1 cut(s)	500
MvaI	CCWGG	1 cut(s)	643
MvnI	CGCG	1 cut(s)	772
MwoI	GCNNNNNNNGC	4 cut(s)	59, 538, 833, 904
NcoI	CCATGG	2 cut(s)	1084, 1207
NdeII	GATC	7 cut(s)	34, 136, 393, 478, 1138, 1142, 1167
NlaIII	CATG	5 cut(s)	52, 166, 235, 1088, 1211
NlaIV	GGNNCC	4 cut(s)	369, 540, 1045, 1125
NmeAIII	GCCGAG	1 cut(s)	567
NmuCI	GTSAC	1 cut(s)	724
NspV	TTCGAA	1 cut(s)	1117
PagI	TCATGA	1 cut(s)	231
PciSI	GCTCTTC	1 cut(s)	958
PcsI	WCGNNNNNNNCGW	2 cut(s)	383, 398
PfeI	GAWTC	3 cut(s)	79, 177, 1114
PkrI	GCNGC	8 cut(s)	306, 418, 421, 801, 829, 900, 1110, 1205
Ple19I	CGATCG	1 cut(s)	37
PpuMI	RGGWCCY	1 cut(s)	1043
Psp5II	RGGWCCY	1 cut(s)	1043
Psp6I	CCWGG	1 cut(s)	641
PspEI	GGTNACC	1 cut(s)	724
PspGI	CCWGG	1 cut(s)	641
PspN4I	GGNNCC	4 cut(s)	369, 540, 1045, 1125
PspPI	GGNCC	3 cut(s)	436, 983, 1043
PspPPI	RGGWCCY	1 cut(s)	1043
PsuI	RGATCY	1 cut(s)	478
PvuI	CGATCG	1 cut(s)	37
RsaI	GTAC	1 cut(s)	364
RsaNI	GTAC	1 cut(s)	363
RseI	CAYNNNNRTG	2 cut(s)	127, 236
SapI	GCTCTTC	1 cut(s)	958
SaqAI	TTAA	3 cut(s)	990, 1197, 1216
SatI	GCNGC	8 cut(s)	305, 417, 420, 800, 828, 899, 1109, 1204
Sau3AI	GATC	7 cut(s)	34, 136, 393, 478, 1138, 1142, 1167
Sau96I	GGNCC	3 cut(s)	436, 983, 1043
ScrFI	CCNGG	1 cut(s)	643
SduI	GDGCHC	1 cut(s)	1128
SfaNI	GCATC	4 cut(s)	228, 290, 760, 811
SfcI	CTRYAG	1 cut(s)	291
SfuI	TTCGAA	1 cut(s)	1117
SinI	GGWCC	2 cut(s)	983, 1043
SmiMI	CAYNNNNRTG	2 cut(s)	127, 236
SpeI	ACTAGT	1 cut(s)	1010
Sse9I	AATT	6 cut(s)	55, 500, 786, 1033, 1069, 1198
SsiI	CCGC	8 cut(s)	360, 419, 668, 770, 800, 830, 981, 1109
SspI	AATATT	1 cut(s)	1176
SspMI	CTAG	2 cut(s)	581, 1011
StyD4I	CCNGG	1 cut(s)	641
StyI	CCWWGG	2 cut(s)	1084, 1207
TaaI	ACNGT	3 cut(s)	295, 721, 965
TaiI	ACGT	1 cut(s)	224
TaqI	TCGA	6 cut(s)	114, 204, 392, 407, 1117, 1141
TasI	AATT	6 cut(s)	55, 500, 786, 1033, 1069, 1198
TauI	GCSGC	3 cut(s)	422, 802, 1111
TfiI	GAWTC	3 cut(s)	79, 177, 1114
Tru1I	TTAA	3 cut(s)	990, 1197, 1216
Tru9I	TTAA	3 cut(s)	990, 1197, 1216
TscAI	CASTG	3 cut(s)	670, 724, 801
TseFI	GTSAC	1 cut(s)	724
TseI	GCWGC	5 cut(s)	304, 416, 827, 898, 1203
Tsp45I	GTSAC	1 cut(s)	724
TspDTI	ATGAA	3 cut(s)	87, 151, 426
TspRI	CASTG	3 cut(s)	670, 724, 801
VpaK11BI	GGWCC	2 cut(s)	983, 1043
XapI	RAATTY	1 cut(s)	55
XmiI	GTMKAC	1 cut(s)	809
XspI	CTAG	2 cut(s)	581, 1011

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.