MD16G1151000.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014718
Physical Location & Seq
Reverse (-)
11906129 .. 11908067
1939 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1151000.v1.1.491

Sequence Viewer

Length: 1116 bp
ATGGATGGTAGAGAAGAAGAGCTAAAGACCACCGATCGTAGCAACGGCATGGAATATTCGACAGTTTTCTCCGACGAGATTCCGACCAACTTTCCTTCTTTTTCCGGCATGTTCGACATGCCATCTTCGGGCGAAGATCAAAAGGTTTCTATGGGAGGGTTCATGGACTTGCATGGAATCCCAGACTACGCTCCTTCTCTATTCGATTTCACTCAAACCACGTCGTCGCTCATGATGATGCCACCACACCACCATCAACAACAATCACTCCCACCTACTTCTACAGTAGTAGCGCTGCCGGAGAGAGATAGTGCTACTACTACTAATACAACCTCGGAGATTTTGAATAGTAGTACGGCGCCTACAACGCCGAACTCGTCTTCGATCTCTTCGTCGTCAAATGAAGCTGCGGCGAATAATAATGAGGGACAAATTACAAAACTGCAGGAAGAGGAAGCCGATGAGCAAGATCCGGAGAAAACCCAGAAACAATTGAAACCGAAAAAGAAGAACCAAAAAAGGCAGAGGGAGCCGAGATTTGCGTTCATGACAAAGAGTGAGGTGGATAACCTAGATGATGGCTACCGATGGCGAAAATACGGCCAAAAAGCTGTTAAAAATAGCCCTTATCCCAGGAGCTATTATCGTTGCACCACTGCGGGCTGTGGTGTGAAGAAGAGGGTGGAGAGGTTGTCCGACGACCCCTCCACTGTGGTCACCACATACGAAGGCCAACACACGCATCCAAGTCCCATTACGCAGCGTGGCACAATGGGAATTGCACGGCTGCCGGATCAGTCTACTGGGTTTATATCTGCTGCGGCTTCATCAAATCCCTTTGCTGTTCAGCAGTTTTTACCTCATCACCGTCACTATCAACAACAACAACAGCAGCAGCAGCAGCAATATGGTTATATGTATCAATCCTCCCCTTCTTTGAATATCAGCTCATCCCCTTACGGTGGTGGTTCTGATGGTGGTCCTTTTAATATCTCCTCATTTTCTACTGGTTTACTTCAAGAGAGAGATATTTTGGAAGGACCCGCGTTTTCTTCTTCGGCTTCCAATTTTCTTAAAGACCATGGACTTCTTCAGGATATGGTACCGCAGATTTGA

Protein Analysis

372

Amino Acids

41.14

Weight (kDa)

5.6

Isoelectric Point (pI)

64.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 192 - 249 1.4e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014718)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g20230
malus_domestica MD13G1150700.v1.1 MD16G1151000.v1.1
prunus_persica Prupe.1G114800_v2.0.a1
pyrus_communis pycom13g12950 pycom16g12920
rosa_chinensis RchiOBHm_Chr4g0425801
rosa_laevigata RLG00000007339
rosa_multiflora Rmu_sc0007063.1_g000001
rosa_roxburghii Rroxscaffold_5G00367790
rosa_rugosa Rorug04G0206200 Rorug04G0206300
rosa_samantha Rh4AG263500 Rh4BG269400 Rh4CG281800 Rh4DG265800
rosa_wichuraiana Rw4G022740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1102
AccB1I GGYRCC 2 cut(s) 358, 1102
AccI GTMKAC 1 cut(s) 800
AccII CGCG 1 cut(s) 1046
AccIII TCCGGA 1 cut(s) 472
AciI CCGC 5 cut(s) 410, 659, 821, 1044, 1106
AclWI GGATC 2 cut(s) 464, 801
AcoI YGGCCR 1 cut(s) 601
AcuI CTGAAG 1 cut(s) 1076
AcyI GRCGYC 1 cut(s) 359
AfaI GTAC 2 cut(s) 355, 1104
AfeI AGCGCT 1 cut(s) 294
AfiI CCNNNNNNNGG 2 cut(s) 128, 962
AgsI TTSAA 4 cut(s) 346, 496, 940, 1019
AjiI CACGTC 1 cut(s) 222
AjnI CCWGG 1 cut(s) 632
AluBI AGCT 5 cut(s) 22, 407, 611, 639, 948
AluI AGCT 5 cut(s) 22, 407, 611, 639, 948
AlwI GGATC 2 cut(s) 464, 801
Aor13HI TCCGGA 1 cut(s) 472
Aor51HI AGCGCT 1 cut(s) 294
AoxI GGCC 2 cut(s) 601, 730
ApeKI GCWGC 9 cut(s) 295, 407, 760, 787, 818, 892, 895, 898, 901
Asp718I GGTACC 1 cut(s) 1102
AspLEI GCGC 2 cut(s) 295, 361
AspS9I GGNCC 2 cut(s) 980, 1040
AsuHPI GGTGA 2 cut(s) 709, 857
AvaII GGWCC 2 cut(s) 980, 1040
BanI GGYRCC 2 cut(s) 358, 1102
BbsI GAAGAC 1 cut(s) 372
BbvI GCAGC 9 cut(s) 282, 394, 772, 774, 805, 904, 907, 910, 913
BccI CCATC 5 cut(s) 130, 261, 572, 582, 968
BceAI ACGGC 4 cut(s) 61, 372, 616, 800
BciT130I CCWGG 1 cut(s) 634
BfaI CTAG 1 cut(s) 572
BfmI CTRYAG 2 cut(s) 282, 443
BfoI RGCGCY 2 cut(s) 296, 362
Bme1390I CCNGG 1 cut(s) 634
Bme18I GGWCC 2 cut(s) 980, 1040
BmgBI CACGTC 1 cut(s) 222
BmgT120I GGNCC 2 cut(s) 980, 1040
BmiI GGNNCC 4 cut(s) 360, 531, 1042, 1104
BmrFI CCNGG 1 cut(s) 634
BmrI ACTGGG 1 cut(s) 813
BmsI GCATC 2 cut(s) 228, 751
BmuI ACTGGG 1 cut(s) 813
BpiI GAAGAC 1 cut(s) 372
BsaHI GRCGYC 1 cut(s) 359
BsaJI CCNNGG 3 cut(s) 333, 632, 1081
BsaWI WCCGGW 1 cut(s) 472
BsaXI ACNNNNNCTCC 6 cut(s) 252, 282, 677, 689, 707, 719
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 962
Bse1I ACTGG 2 cut(s) 808, 1012
BseAI TCCGGA 1 cut(s) 472
BseBI CCWGG 1 cut(s) 634
BseDI CCNNGG 3 cut(s) 333, 632, 1081
BseGI GGATG 3 cut(s) 10, 742, 950
BseLI CCNNNNNNNGG 2 cut(s) 128, 962
BseNI ACTGG 2 cut(s) 808, 1012
BseRI GAGGAG 1 cut(s) 985
BseXI GCAGC 9 cut(s) 282, 394, 772, 774, 805, 904, 907, 910, 913
Bsh1236I CGCG 1 cut(s) 1046
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 2 cut(s) 603, 732
BshNI GGYRCC 2 cut(s) 358, 1102
BsiEI CGRYCG 1 cut(s) 37
BsiSI CCGG 4 cut(s) 105, 299, 473, 791
BslFI GGGAC 2 cut(s) 441, 735
BslI CCNNNNNNNGG 2 cut(s) 128, 962
BsmFI GGGAC 2 cut(s) 441, 735
BsnI GGCC 2 cut(s) 603, 732
Bsp13I TCCGGA 1 cut(s) 472
Bsp143I GATC 5 cut(s) 34, 136, 384, 469, 793
Bsp19I CCATGG 1 cut(s) 1081
BspACI CCGC 5 cut(s) 410, 659, 821, 1044, 1106
BspANI GGCC 2 cut(s) 603, 732
BspEI TCCGGA 1 cut(s) 472
BspFNI CGCG 1 cut(s) 1046
BspHI TCATGA 2 cut(s) 231, 546
BspLI GGNNCC 4 cut(s) 360, 531, 1042, 1104
BspMAI CTGCAG 1 cut(s) 447
BspPI GGATC 2 cut(s) 464, 801
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 2 cut(s) 358, 1102
BsrI ACTGG 2 cut(s) 808, 1012
BssECI CCNNGG 3 cut(s) 333, 632, 1081
BssMI GATC 5 cut(s) 34, 136, 384, 469, 793
BssNI GRCGYC 1 cut(s) 359
BssT1I CCWWGG 1 cut(s) 1081
Bst2UI CCWGG 1 cut(s) 634
Bst4CI ACNGT 5 cut(s) 64, 286, 712, 869, 962
Bst6I CTCTTC 4 cut(s) 12, 394, 444, 671
BstACI GRCGYC 1 cut(s) 359
BstC8I GCNNGC 1 cut(s) 661
BstDSI CCRYGG 1 cut(s) 1081
BstEII GGTNACC 1 cut(s) 715
BstF5I GGATG 3 cut(s) 10, 742, 950
BstFNI CGCG 1 cut(s) 1046
BstH2I RGCGCY 2 cut(s) 296, 362
BstHHI GCGC 2 cut(s) 295, 361
BstKTI GATC 5 cut(s) 37, 139, 387, 472, 796
BstMBI GATC 5 cut(s) 34, 136, 384, 469, 793
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 4 cut(s) 367, 529, 898, 901
BstNI CCWGG 1 cut(s) 634
BstNSI RCATGY 2 cut(s) 112, 121
BstPI GGTNACC 1 cut(s) 715
BstSCI CCNGG 1 cut(s) 632
BstSFI CTRYAG 2 cut(s) 282, 443
BstUI CGCG 1 cut(s) 1046
BstV1I GCAGC 9 cut(s) 282, 394, 772, 774, 805, 904, 907, 910, 913
BstV2I GAAGAC 1 cut(s) 372
BstX2I RGATCY 1 cut(s) 469
BstYI RGATCY 1 cut(s) 469
BsuRI GGCC 2 cut(s) 603, 732
BtgI CCRYGG 1 cut(s) 1081
BtrI CACGTC 1 cut(s) 222
BtsCI GGATG 3 cut(s) 10, 742, 950
BtsI GCAGTG 1 cut(s) 654
BtsIMutI CAGTG 2 cut(s) 654, 708
Cac8I GCNNGC 1 cut(s) 661
CciI TCATGA 2 cut(s) 231, 546
CfoI GCGC 2 cut(s) 295, 361
Cfr13I GGNCC 2 cut(s) 980, 1040
Csp6I GTAC 2 cut(s) 354, 1103
CviAII CATG 8 cut(s) 49, 109, 118, 163, 173, 232, 547, 1082
CviQI GTAC 2 cut(s) 354, 1103
DinI GGCGCC 1 cut(s) 360
DpnI GATC 5 cut(s) 36, 138, 386, 471, 795
DpnII GATC 5 cut(s) 34, 136, 384, 469, 793
EaeI YGGCCR 1 cut(s) 601
Eam1104I CTCTTC 4 cut(s) 12, 394, 444, 671
EarI CTCTTC 4 cut(s) 12, 394, 444, 671
Eco130I CCWWGG 1 cut(s) 1081
Eco47I GGWCC 2 cut(s) 980, 1040
Eco47III AGCGCT 1 cut(s) 294
Eco57I CTGAAG 1 cut(s) 1076
Eco91I GGTNACC 1 cut(s) 715
EcoO109I RGGNCCY 1 cut(s) 1040
EcoO65I GGTNACC 1 cut(s) 715
EcoRII CCWGG 1 cut(s) 632
EcoT14I CCWWGG 1 cut(s) 1081
EgeI GGCGCC 1 cut(s) 360
EheI GGCGCC 1 cut(s) 360
ErhI CCWWGG 1 cut(s) 1081
FaeI CATG 8 cut(s) 52, 112, 121, 166, 176, 235, 550, 1085
FaqI GGGAC 2 cut(s) 441, 735
FatI CATG 8 cut(s) 48, 108, 117, 162, 172, 231, 546, 1081
FauI CCCGC 2 cut(s) 652, 1051
FblI GTMKAC 1 cut(s) 800
FokI GGATG 3 cut(s) 17, 729, 937
FspBI CTAG 1 cut(s) 572
GlaI GCGC 2 cut(s) 294, 360
HaeII RGCGCY 2 cut(s) 296, 362
HaeIII GGCC 2 cut(s) 603, 732
HapII CCGG 4 cut(s) 105, 299, 473, 791
HhaI GCGC 2 cut(s) 295, 361
Hin1I GRCGYC 1 cut(s) 359
Hin1II CATG 8 cut(s) 52, 112, 121, 166, 176, 235, 550, 1085
Hin6I GCGC 2 cut(s) 293, 359
HinP1I GCGC 2 cut(s) 293, 359
HinfI GANTC 2 cut(s) 79, 177
HpaII CCGG 4 cut(s) 105, 299, 473, 791
HphI GGTGA 2 cut(s) 709, 857
Hpy166II GTNNAC 2 cut(s) 801, 1013
Hpy188I TCNGA 5 cut(s) 73, 84, 337, 697, 973
Hpy188III TCNNGA 5 cut(s) 232, 473, 547, 1019, 1094
Hpy8I GTNNAC 2 cut(s) 801, 1013
Hpy99I CGWCG 5 cut(s) 77, 226, 229, 397, 701
HpyAV CCTTC 5 cut(s) 105, 204, 722, 942, 1031
HpyCH4III ACNGT 5 cut(s) 64, 286, 712, 869, 962
HpyCH4IV ACGT 1 cut(s) 221
HpyCH4V TGCA 4 cut(s) 172, 445, 651, 782
HpyF10VI GCNNNNNNNGC 4 cut(s) 367, 529, 898, 901
HpySE526I ACGT 1 cut(s) 221
Hsp92I GRCGYC 1 cut(s) 359
Hsp92II CATG 8 cut(s) 52, 112, 121, 166, 176, 235, 550, 1085
HspAI GCGC 2 cut(s) 293, 359
KasI GGCGCC 1 cut(s) 358
Kpn2I TCCGGA 1 cut(s) 472
KpnI GGTACC 1 cut(s) 1106
Kzo9I GATC 5 cut(s) 34, 136, 384, 469, 793
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 3 cut(s) 196, 529, 636
Lsp1109I GCAGC 9 cut(s) 282, 394, 772, 774, 805, 904, 907, 910, 913
LweI GCATC 2 cut(s) 228, 751
MaeI CTAG 1 cut(s) 572
MaeII ACGT 1 cut(s) 221
MaeIII GTNAC 2 cut(s) 715, 869
MalI GATC 5 cut(s) 36, 138, 386, 471, 795
MboI GATC 5 cut(s) 34, 136, 384, 469, 793
MfeI CAATTG 1 cut(s) 491
MflI RGATCY 1 cut(s) 469
MluCI AATT 4 cut(s) 432, 491, 777, 1066
Mly113I GGCGCC 1 cut(s) 359
MmeI TCCRAC 3 cut(s) 96, 107, 720
MroI TCCGGA 1 cut(s) 472
MseI TTAA 3 cut(s) 615, 987, 1074
MslI CAYNNNNRTG 1 cut(s) 236
MspI CCGG 4 cut(s) 105, 299, 473, 791
MspR9I CCNGG 1 cut(s) 634
MunI CAATTG 1 cut(s) 491
MvaI CCWGG 1 cut(s) 634
MvnI CGCG 1 cut(s) 1046
MwoI GCNNNNNNNGC 4 cut(s) 367, 529, 898, 901
NarI GGCGCC 1 cut(s) 359
NcoI CCATGG 1 cut(s) 1081
NdeII GATC 5 cut(s) 34, 136, 384, 469, 793
NlaIII CATG 8 cut(s) 52, 112, 121, 166, 176, 235, 550, 1085
NlaIV GGNNCC 4 cut(s) 360, 531, 1042, 1104
NmeAIII GCCGAG 1 cut(s) 558
NmuCI GTSAC 2 cut(s) 715, 869
NspI RCATGY 2 cut(s) 112, 121
PagI TCATGA 2 cut(s) 231, 546
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 2 cut(s) 374, 389
PfeI GAWTC 2 cut(s) 79, 177
Ple19I CGATCG 1 cut(s) 37
PluTI GGCGCC 1 cut(s) 362
PpuMI RGGWCCY 1 cut(s) 1040
Psp5II RGGWCCY 1 cut(s) 1040
Psp6I CCWGG 1 cut(s) 632
PspEI GGTNACC 1 cut(s) 715
PspGI CCWGG 1 cut(s) 632
PspN4I GGNNCC 4 cut(s) 360, 531, 1042, 1104
PspPI GGNCC 2 cut(s) 980, 1040
PspPPI RGGWCCY 1 cut(s) 1040
PstI CTGCAG 1 cut(s) 447
PsuI RGATCY 1 cut(s) 469
PvuI CGATCG 1 cut(s) 37
RsaI GTAC 2 cut(s) 355, 1104
RsaNI GTAC 2 cut(s) 354, 1103
RseI CAYNNNNRTG 1 cut(s) 236
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 3 cut(s) 615, 987, 1074
Sau3AI GATC 5 cut(s) 34, 136, 384, 469, 793
Sau96I GGNCC 2 cut(s) 980, 1040
ScrFI CCNGG 1 cut(s) 634
SfaNI GCATC 2 cut(s) 228, 751
SfcI CTRYAG 2 cut(s) 282, 443
SfoI GGCGCC 1 cut(s) 360
SinI GGWCC 2 cut(s) 980, 1040
SmiMI CAYNNNNRTG 1 cut(s) 236
Sse9I AATT 4 cut(s) 432, 491, 777, 1066
SsiI CCGC 5 cut(s) 410, 659, 821, 1044, 1106
SspDI GGCGCC 1 cut(s) 358
SspI AATATT 1 cut(s) 56
SspMI CTAG 1 cut(s) 572
StyD4I CCNGG 1 cut(s) 632
StyI CCWWGG 1 cut(s) 1081
TaaI ACNGT 5 cut(s) 64, 286, 712, 869, 962
TaiI ACGT 1 cut(s) 224
TaqI TCGA 4 cut(s) 59, 114, 204, 383
TasI AATT 4 cut(s) 432, 491, 777, 1066
TauI GCSGC 2 cut(s) 413, 824
TfiI GAWTC 2 cut(s) 79, 177
Tru1I TTAA 3 cut(s) 615, 987, 1074
Tru9I TTAA 3 cut(s) 615, 987, 1074
TscAI CASTG 2 cut(s) 661, 715
TseFI GTSAC 2 cut(s) 715, 869
TseI GCWGC 9 cut(s) 295, 407, 760, 787, 818, 892, 895, 898, 901
Tsp45I GTSAC 2 cut(s) 715, 869
TspDTI ATGAA 4 cut(s) 151, 417, 535, 816
TspRI CASTG 2 cut(s) 661, 715
VpaK11BI GGWCC 2 cut(s) 980, 1040
XceI RCATGY 2 cut(s) 112, 121
XmiI GTMKAC 1 cut(s) 800
XspI CTAG 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.