Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0014718

Physical Location & Seq

Genomic Coordinates Forward (+)

35885771 .. 35891816

Gene Structure 6046 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0206300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3204 bp

ATGGCGGGAAATGAGTGGATCAATGGCTACTTGGAGGCGATACTTGACACCGGGGCGACGGCGATCGAGGAGCAGAAGCCGATGCCGGTGAATCTGAGAGAGGGAGGACATTTTAATCCGACTAAGTATTTTGTGGAGGAGGTGGTTACTGGTGTGGATGAGTCGGATTTGTACCGGACGTGGATCAAAGTGGTTGCTACACGCAACACACGAGAGCGTAGCTCCAGGCTCGAGAACATGTGCTGGCGCATTTGGCATCTTACCCGTAAGAAGAAACAGTTGGAGTGGGAGGAACTTCAACGGTCGGCAAACCGCAGATGGGAGCGGGAACAAGGACGGAGGGATGCAACCGAAGACATGTCAGAAGACTTGTCCGAAGGAGAGAAGGGTGATGCTTTGGCAGAGATGCTGCTATGTGAGACTCCAAGAAGAAAGTTCCAGCGAATGGTTTCTAATTTGGAAGTATGGTCAGATGATAAAAAGGAAAAGAAACTATATGTCGTTCTGATCAGTTTGCATGGTTTAGTCCGGGGAGAAAACATGGAGCTTGGTCGAGACTCAGATACAGGTGGACAGGTCAAATATGTGGTAGAGCTTGCCAGAGCACTTGCAAGGATGCCTGGGGTTTATAGGGTAGATCTCTTTACTCGCCAAGTGTCATCTCCAGAGGTTGATTGGAGCTATGGTGAACCAACAGAGATGATAACTGCAGGTCCTGAAGATGGTGATGGTGATCTAGGAGAAAGCAGCGGAGCATATATAATCAGAATTCCTTTTGGTCCTCGCGACCAATACCTCAGTAAAGAAGTACTCTGGCCCCATATTCAAGAATTTGTGGATGGGGCTCTAGCTCACATTCTTAATATGTCCAAAGTTTTGGGTGAACAAATTGGGAGAGGCCAGCCTGTCTGGCCATATGTAATTCATGGCCACTATGCAGATGCAGGGGATAGTGCTGCTCTTCTTTCTGGTGCTTTGAATGTCCCAATGGTTCTAACAGGACACTCACTTGGAAGAAACAAGCTTGAACAGCTTATTAAGCAGGGACGACAATCAAAGGAAGACATTAATTCAACATATAAGATTATGAGAAGGATAGAGGCGGAAGAGCTTTCCCTTGATGCTGCAGAACTTGTTATTACAAGTACAAAACAGGAAATCGAAGAGCAATGGGGACTTTATGATGGGTTTGATGTCAAGCTTGAGAAAGTTTTGCGTGCCCGGGCTAGAAGAGGAGTAAATTGCCATGGTCGTTACATGCCAAGGATGGTGGTTATACCTCCTGGCATGGACTTCAGTAATGTTATGGTCCAAGAAGATGATGCTGATGGGGAACTTAGCCAGCTTATTGGTGGTACCAGTGATGGGTCTTCTTCCCCAAAAGCAATACCAACAATCTGGTCAGAAGTGATGAGATTCCTTACAAATCCTCACAAGCCAATGATCTTGGCTCTGTCACGGCCAGATCCAAAGAAGAACATAACCACTCTTTTGAAAGCCTTTGGAGAATGCCGTCCTCTAAGAGAACTTGCTAACCTTACACTGATAATGGGGAATAGGGACTATATAGACGAAATGTCTACCGGAAATGCAAGTGTGCTTACTACAGTGTTGAAAATGATTGATAAGTATGATCTTTATGGGCAAGTGGCCTATCCAAAGCATCACAAGCAATCAGATGTTCCAGATATTTACCGGCTTGCAGCAAAAACAAAGGGAGTTTTCATAAATCCAGCATTAGTGGAGCCTTTTGGGCTAACCTTGATTGAGGCAGCAGCACACGGGCTTCCGATGGTGGCTACAAAAAATGGTGGACCGGTTGACATACATCGGGCGCTTAGTAATGGTTTGCTTGTGGACCCTCATGATCAGCAATCAATTGCTAATGCACTGCTGAAGTTGTTATCGGAAAAGAATTTATGGGTTGAGTGCAGAAAGAATGGTTGGAAAAACATACACCTTTTCTCATGGCCTGAACACTGCCGAACCTACTTGACTAGAGTAGCAGCCTGCCGCATGAGACATCCACAGTGGCAGACTGACACCCCAGAGGATGAAATGGCTGCTGAAGAGTCCCTCAATGATTCTCTTAAGGACGTGCAAGATATGTCTCTTAGGCTCTCAGTTGATGGGGACAAATCCTCACTGAATGAATCTCTTGATGTGACTGCAGCTTCTGGTGACCATGAGGTGCAAGATCAAGTGAAGCGTGTCCTAAGCAAGATCAAGAAACCAGATTCTGGTCCAAAAGACCATGAAGAAGGAAATAAGCTGCCGGATAATGTAGCAAGCAAATATCCCTTGTTAAGACGTAGAAGAAAATTGATTGTCATAGCACTCGATTGCTATGACAAATCTGGAGCTCCTGAAAAGAAAATGATTCAAATTGTGCAAGAAATATTTAAGGCTGTAAGACTAGATTCTCAAAGTGCAAGATTTACAGGGTTTGCTCTGCTAACAGCTATGCCAGCATCAGAAACAGTAGAGTTTCTGGCATCAGGGAGGGTACAGGCAAATGAGTTTGATGCGTTGGTTTGTAGCAGTGGAAGTGAGGTTTACTACCCTGGTACTTATACAGAAGAAGATGGAAGACTTTTCCCAGATCCAGATTATTCGTCACACATTGACTACCGATGGGGTTGTGAAGGATTAAAGAAAACCATTTGGAAGCTCTTAAATGGCCCTGATGGTGAACGGAATTCCACATCTTCGAGCCACATTGAAGAAGATATAAAATCAAGCAGTGCCCATTGCATTTCATACTTGATCAAGGATCCTAGTAAGGCAAGGAAAGTTGATGATTTAAGACAGAAACTCAGGATGCGGGGACTACGCTGCCATCCAATGTACTGCAGGAGCTCAACGCGAATGCAAATTGTTCCTCTCCTTGCTTCTCGAGCACAGGCTCTCAGGTACCTTTTCGTACGCTGGAGATTGAATGTCGCAAACATGTATGTGTTCCTTGGAGAAAGTGGAGACACTGATTATGAAGAAATGATAGCAGGGACTCACAAGACCATAATCATGAAAGGGGTGGTGGGAAAGGGTTCTGAAGAGCTGCTAAGAACATCAGGAAGCTATCTGAGAGACGACATTGTTCCTCCTCAGAGCCCACTGGTCGCCATTGTAAATGGGCAAGCTCCAACAGCTGATGAGATTGCCACTGCTCTGAAGCAAGTCACCAAATCTGCTGCTGGAATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009891 GO:0009893 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0098542 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

1067

Amino Acids

119.9

Weight (kDa)

6.1

Isoelectric Point (pI)

47.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GT-B_Sucrose_synth	PF00862	166 - 432	2e-11	Sucrose synthase first GT-B domain
Glyco_trans_4_4	PF13579	190 - 388	1e-06	Glycosyl transferase 4-like domain
Glycos_transf_1	PF00534	476 - 648	1.5e-23	Glycosyl transferases group 1
Glyco_trans_1_4	PF13692	480 - 636	2.3e-15	Glycosyl transferases group 1
S6PP	PF05116	842 - 1021	7.8e-13	Sucrose-6F-phosphate phosphohydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0014718)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g20230
malus_domestica	MD13G1150700.v1.1 MD16G1151000.v1.1
prunus_persica	Prupe.1G114800_v2.0.a1
pyrus_communis	pycom13g12950 pycom16g12920
rosa_chinensis	RchiOBHm_Chr4g0425801
rosa_laevigata	RLG00000007339
rosa_multiflora	Rmu_sc0007063.1_g000001
rosa_roxburghii	Rroxscaffold_5G00367790
rosa_rugosa	Rorug04G0206200 Rorug04G0206300
rosa_samantha	Rh4AG263500 Rh4BG269400 Rh4CG281800 Rh4DG265800
rosa_wichuraiana	Rw4G022740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	701
Acc65I	GGTACC	2 cut(s)	1355, 2913
AccB1I	GGYRCC	2 cut(s)	1355, 2913
AccB7I	CCANNNNNTGG	2 cut(s)	445, 2240
AccBSI	CCGCTC	1 cut(s)	325
AccI	GTMKAC	1 cut(s)	1580
AccII	CGCG	2 cut(s)	786, 2866
AciI	CCGC	7 cut(s)	5, 313, 325, 750, 1103, 2014, 2824
AclWI	GGATC	6 cut(s)	26, 191, 1460, 2597, 2768, 2781
AcoI	YGGCCR	3 cut(s)	911, 928, 1460
AcsI	RAATTY	4 cut(s)	768, 830, 1915, 2698
AcuI	CTGAAG	6 cut(s)	738, 1279, 1916, 2088, 3072, 3191
AfaI	GTAC	9 cut(s)	173, 810, 1147, 1357, 2508, 2569, 2849, 2915, 2925
AfiI	CCNNNNNNNGG	5 cut(s)	319, 445, 722, 1365, 2240
AflII	CTTAAG	1 cut(s)	2090
AflIII	ACRYGT	3 cut(s)	237, 357, 2949
AgeI	ACCGGT	1 cut(s)	1816
AhdI	GACNNNNNGTC	1 cut(s)	1576
AjiI	CACGTC	2 cut(s)	180, 2098
AjnI	CCWGG	4 cut(s)	224, 619, 1282, 2563
AjuI	GAANNNNNNNTTGG	4 cut(s)	263, 295, 1927, 1959
Alw21I	GWGCWC	4 cut(s)	607, 2365, 2861, 2902
Alw26I	GTCTC	6 cut(s)	413, 549, 2014, 2115, 2970, 3081
AlwI	GGATC	6 cut(s)	26, 191, 1460, 2597, 2768, 2781
AlwNI	CAGNNNCTG	3 cut(s)	716, 2177, 2240
Ama87I	CYCGRG	3 cut(s)	230, 1221, 2895
AoxI	GGCC	8 cut(s)	815, 898, 911, 928, 1460, 1650, 1970, 2680
ApoI	RAATTY	4 cut(s)	768, 830, 1915, 2698
AseI	ATTAAT	1 cut(s)	1068
AsiGI	ACCGGT	1 cut(s)	1816
Asp700I	GAANNNNTTC	2 cut(s)	448, 3046
Asp718I	GGTACC	2 cut(s)	1355, 2913
AspLEI	GCGC	2 cut(s)	249, 1837
AspS9I	GGNCC	8 cut(s)	713, 779, 816, 1309, 1814, 1858, 2243, 2681
AsuC2I	CCSGG	4 cut(s)	52, 530, 1222, 1223
AsuHPI	GGTGA	9 cut(s)	100, 401, 698, 737, 743, 893, 2192, 2702, 3172
AvaI	CYCGRG	3 cut(s)	230, 1221, 2895
AvaII	GGWCC	6 cut(s)	713, 779, 1309, 1814, 1858, 2243
BaeGI	GKGCMC	2 cut(s)	1222, 2749
BalI	TGGCCA	2 cut(s)	913, 930
BamHI	GGATCC	1 cut(s)	2773
BanI	GGYRCC	2 cut(s)	1355, 2913
BanII	GRGCYC	4 cut(s)	847, 2365, 2861, 3113
BauI	CACGAG	1 cut(s)	210
BbsI	GAAGAC	5 cut(s)	360, 372, 1068, 1362, 2596
Bbv12I	GWGCWC	4 cut(s)	607, 2365, 2861, 2902
BceAI	ACGGC	3 cut(s)	75, 1475, 1497
BciT130I	CCWGG	4 cut(s)	226, 621, 1284, 2565
BclI	TGATCA	3 cut(s)	507, 1867, 2766
BcnI	CCSGG	4 cut(s)	52, 530, 1222, 1223
BcoDI	GTCTC	6 cut(s)	413, 549, 2014, 2115, 2970, 3081
BfaI	CTAG	6 cut(s)	737, 848, 1227, 1998, 2417, 2778
BfmI	CTRYAG	5 cut(s)	708, 1125, 1605, 2169, 2851
BfoI	RGCGCY	1 cut(s)	1838
BfrI	CTTAAG	1 cut(s)	2090
BfuAI	ACCTGC	1 cut(s)	701
BglI	GCCNNNNNGGC	2 cut(s)	910, 1753
BglII	AGATCT	1 cut(s)	637
BmcAI	AGTACT	1 cut(s)	810
Bme1390I	CCNGG	8 cut(s)	52, 226, 530, 621, 1222, 1223, 1284, 2565
Bme18I	GGWCC	6 cut(s)	713, 779, 1309, 1814, 1858, 2243
BmeRI	GACNNNNNGTC	1 cut(s)	1576
BmeT110I	CYCGRG	3 cut(s)	230, 1221, 2895
BmgBI	CACGTC	2 cut(s)	180, 2098
BmgT120I	GGNCC	8 cut(s)	713, 779, 816, 1309, 1814, 1858, 2243, 2681
BmiI	GGNNCC	6 cut(s)	818, 1357, 1746, 1860, 2775, 2915
BmrFI	CCNGG	8 cut(s)	52, 226, 530, 621, 1222, 1223, 1284, 2565
BpiI	GAAGAC	5 cut(s)	360, 372, 1068, 1362, 2596
BplI	GAGNNNNNCTC	2 cut(s)	206, 238
BpmI	CTGGAG	4 cut(s)	208, 648, 2379, 2950
Bpu10I	CCTNAGC	1 cut(s)	2216
BpuEI	CTTGAG	1 cut(s)	1223
BpuMI	CCSGG	4 cut(s)	52, 530, 1222, 1223
BsaBI	GATNNNNATC	1 cut(s)	732
BsaJI	CCNNGG	8 cut(s)	51, 529, 620, 1221, 1246, 1262, 2563, 2962
BsaWI	WCCGGW	3 cut(s)	174, 1583, 1816
BsaXI	ACNNNNNCTCC	2 cut(s)	670, 700
Bsc4I	CCNNNNNNNGG	5 cut(s)	319, 445, 722, 1365, 2240
Bse118I	RCCGGY	3 cut(s)	85, 1695, 1816
Bse1I	ACTGG	3 cut(s)	154, 1359, 3120
Bse3DI	GCAATG	2 cut(s)	1175, 2749
Bse8I	GATNNNNATC	1 cut(s)	732
BseBI	CCWGG	4 cut(s)	226, 621, 1284, 2565
BseDI	CCNNGG	8 cut(s)	51, 529, 620, 1221, 1246, 1262, 2563, 2962
BseGI	GGATG	9 cut(s)	163, 349, 621, 844, 1272, 2023, 2059, 2826, 2839
BseJI	GATNNNNATC	1 cut(s)	732
BseLI	CCNNNNNNNGG	5 cut(s)	319, 445, 722, 1365, 2240
BseMI	GCAATG	2 cut(s)	1175, 2749
BseMII	CTCAG	8 cut(s)	86, 573, 811, 2136, 2830, 2923, 3074, 3119
BseNI	ACTGG	3 cut(s)	154, 1359, 3120
BseRI	GAGGAG	4 cut(s)	83, 152, 1248, 3093
BseSI	GKGCMC	2 cut(s)	1222, 2749
BsgI	GTGCAG	1 cut(s)	1951
Bsh1236I	CGCG	2 cut(s)	786, 2866
Bsh1285I	CGRYCG	2 cut(s)	66, 305
BshFI	GGCC	8 cut(s)	817, 900, 913, 930, 1462, 1652, 1972, 2682
BshNI	GGYRCC	2 cut(s)	1355, 2913
BshTI	ACCGGT	1 cut(s)	1816
BsiEI	CGRYCG	2 cut(s)	66, 305
BsiHKAI	GWGCWC	4 cut(s)	607, 2365, 2861, 2902
BsiHKCI	CYCGRG	3 cut(s)	230, 1221, 2895
BsiSI	CCGG	9 cut(s)	51, 86, 175, 529, 1222, 1584, 1696, 1817, 2276
BsiWI	CGTACG	1 cut(s)	2923
BslFI	GGGAC	8 cut(s)	968, 1059, 1188, 1574, 2059, 2147, 2841, 3019
BslI	CCNNNNNNNGG	5 cut(s)	319, 445, 722, 1365, 2240
BsmAI	GTCTC	6 cut(s)	413, 549, 2014, 2115, 2970, 3081
BsmBI	CGTCTC	1 cut(s)	3081
BsmFI	GGGAC	8 cut(s)	968, 1059, 1188, 1574, 2059, 2147, 2841, 3019
BsmI	GAATGC	2 cut(s)	1514, 2874
BsnI	GGCC	8 cut(s)	817, 900, 913, 930, 1462, 1652, 1972, 2682
BsoBI	CYCGRG	3 cut(s)	230, 1221, 2895
Bsp1286I	GDGCHC	8 cut(s)	607, 847, 1222, 2365, 2749, 2861, 2902, 3113
Bsp19I	CCATGG	1 cut(s)	1246
Bsp68I	TCGCGA	1 cut(s)	786
BspACI	CCGC	7 cut(s)	5, 313, 325, 750, 1103, 2014, 2824
BspANI	GGCC	8 cut(s)	817, 900, 913, 930, 1462, 1652, 1972, 2682
BspCNI	CTCAG	8 cut(s)	87, 572, 810, 2135, 2829, 2922, 3075, 3118
BspFNI	CGCG	2 cut(s)	786, 2866
BspHI	TCATGA	2 cut(s)	1864, 3024
BspLI	GGNNCC	6 cut(s)	818, 1357, 1746, 1860, 2775, 2915
BspMAI	CTGCAG	4 cut(s)	712, 1129, 2173, 2855
BspMI	ACCTGC	1 cut(s)	701
BspPI	GGATC	6 cut(s)	26, 191, 1460, 2597, 2768, 2781
BspQI	GCTCTTC	4 cut(s)	966, 1101, 1158, 3048
BspT107I	GGYRCC	2 cut(s)	1355, 2913
BspTI	CTTAAG	1 cut(s)	2090
BsrBI	CCGCTC	1 cut(s)	325
BsrDI	GCAATG	2 cut(s)	1175, 2749
BsrFI	RCCGGY	3 cut(s)	85, 1695, 1816
BsrI	ACTGG	3 cut(s)	154, 1359, 3120
BssAI	RCCGGY	3 cut(s)	85, 1695, 1816
BssECI	CCNNGG	8 cut(s)	51, 529, 620, 1221, 1246, 1262, 2563, 2962
BssSI	CACGAG	1 cut(s)	210
BssT1I	CCWWGG	3 cut(s)	1246, 1262, 2962
Bst2BI	CACGAG	1 cut(s)	210
Bst2UI	CCWGG	4 cut(s)	226, 621, 1284, 2565
Bst4CI	ACNGT	5 cut(s)	279, 303, 1609, 2031, 2482
Bst6I	CTCTTC	6 cut(s)	966, 1101, 1158, 1225, 2064, 3048
BstAFI	CTTAAG	1 cut(s)	2090
BstDSI	CCRYGG	1 cut(s)	1246
BstEII	GGTNACC	1 cut(s)	2180
BstF5I	GGATG	9 cut(s)	163, 349, 621, 844, 1272, 2023, 2059, 2826, 2839
BstFNI	CGCG	2 cut(s)	786, 2866
BstH2I	RGCGCY	1 cut(s)	1838
BstHHI	GCGC	2 cut(s)	249, 1837
BstMAI	GTCTC	6 cut(s)	413, 549, 2014, 2115, 2970, 3081
BstMCI	CGRYCG	2 cut(s)	66, 305
BstMWI	GCNNNNNNNGC	9 cut(s)	253, 910, 1030, 1039, 1669, 1753, 2468, 2897, 3146
BstNI	CCWGG	4 cut(s)	226, 621, 1284, 2565
BstNSI	RCATGY	4 cut(s)	241, 361, 1261, 2953
BstPI	GGTNACC	1 cut(s)	2180
BstSCI	CCNGG	8 cut(s)	50, 224, 528, 619, 1220, 1221, 1282, 2563
BstSFI	CTRYAG	5 cut(s)	708, 1125, 1605, 2169, 2851
BstSLI	GKGCMC	2 cut(s)	1222, 2749
BstUI	CGCG	2 cut(s)	786, 2866
BstV2I	GAAGAC	5 cut(s)	360, 372, 1068, 1362, 2596
BstX2I	RGATCY	4 cut(s)	637, 1465, 2602, 2773
BstXI	CCANNNNNNTGG	3 cut(s)	877, 1349, 1398
BstYI	RGATCY	4 cut(s)	637, 1465, 2602, 2773
BsuRI	GGCC	8 cut(s)	817, 900, 913, 930, 1462, 1652, 1972, 2682
BtgI	CCRYGG	1 cut(s)	1246
BtrI	CACGTC	2 cut(s)	180, 2098
BtsCI	GGATG	9 cut(s)	163, 349, 621, 844, 1272, 2023, 2059, 2826, 2839
BtsI	GCAGTG	5 cut(s)	1889, 1978, 2548, 2749, 3162
BtuMI	TCGCGA	1 cut(s)	786
BveI	ACCTGC	1 cut(s)	701
CaiI	CAGNNNCTG	3 cut(s)	716, 2177, 2240
CciI	TCATGA	2 cut(s)	1864, 3024
CfoI	GCGC	2 cut(s)	249, 1837
Cfr10I	RCCGGY	3 cut(s)	85, 1695, 1816
Cfr13I	GGNCC	8 cut(s)	713, 779, 816, 1309, 1814, 1858, 2243, 2681
Cfr9I	CCCGGG	1 cut(s)	1221
Csp6I	GTAC	9 cut(s)	172, 809, 1146, 1356, 2507, 2568, 2848, 2914, 2924
CspAI	ACCGGT	1 cut(s)	1816
CspCI	CAANNNNNGTGG	2 cut(s)	1251, 1286
CviQI	GTAC	9 cut(s)	172, 809, 1146, 1356, 2507, 2568, 2848, 2914, 2924
DriI	GACNNNNNGTC	1 cut(s)	1576
EaeI	YGGCCR	3 cut(s)	911, 928, 1460
Eam1104I	CTCTTC	6 cut(s)	966, 1101, 1158, 1225, 2064, 3048
Eam1105I	GACNNNNNGTC	1 cut(s)	1576
EarI	CTCTTC	6 cut(s)	966, 1101, 1158, 1225, 2064, 3048
EciI	GGCGGA	1 cut(s)	1118
Ecl136II	GAGCTC	2 cut(s)	2363, 2859
Eco130I	CCWWGG	3 cut(s)	1246, 1262, 2962
Eco24I	GRGCYC	4 cut(s)	847, 2365, 2861, 3113
Eco47I	GGWCC	6 cut(s)	713, 779, 1309, 1814, 1858, 2243
Eco53kI	GAGCTC	2 cut(s)	2363, 2859
Eco57I	CTGAAG	6 cut(s)	738, 1279, 1916, 2088, 3072, 3191
Eco88I	CYCGRG	3 cut(s)	230, 1221, 2895
Eco91I	GGTNACC	1 cut(s)	2180
EcoICRI	GAGCTC	2 cut(s)	2363, 2859
EcoO109I	RGGNCCY	1 cut(s)	713
EcoO65I	GGTNACC	1 cut(s)	2180
EcoRI	GAATTC	2 cut(s)	768, 2698
EcoRII	CCWGG	4 cut(s)	224, 619, 1282, 2563
EcoT14I	CCWWGG	3 cut(s)	1246, 1262, 2962
EcoT38I	GRGCYC	4 cut(s)	847, 2365, 2861, 3113
ErhI	CCWWGG	3 cut(s)	1246, 1262, 2962
Esp3I	CGTCTC	1 cut(s)	3081
FaqI	GGGAC	8 cut(s)	968, 1059, 1188, 1574, 2059, 2147, 2841, 3019
FauI	CCCGC	2 cut(s)	318, 2817
FauNDI	CATATG	1 cut(s)	916
FbaI	TGATCA	3 cut(s)	507, 1867, 2766
FblI	GTMKAC	1 cut(s)	1580
FokI	GGATG	9 cut(s)	170, 356, 628, 851, 1279, 2010, 2066, 2826, 2833
FriOI	GRGCYC	4 cut(s)	847, 2365, 2861, 3113
FspBI	CTAG	6 cut(s)	737, 848, 1227, 1998, 2417, 2778
GlaI	GCGC	2 cut(s)	248, 1836
GsuI	CTGGAG	4 cut(s)	208, 648, 2379, 2950
HaeII	RGCGCY	1 cut(s)	1838
HaeIII	GGCC	8 cut(s)	817, 900, 913, 930, 1462, 1652, 1972, 2682
HapII	CCGG	9 cut(s)	51, 86, 175, 529, 1222, 1584, 1696, 1817, 2276
HhaI	GCGC	2 cut(s)	249, 1837
Hin6I	GCGC	2 cut(s)	247, 1835
HinP1I	GCGC	2 cut(s)	247, 1835
HincII	GTYRAC	1 cut(s)	1822
HindII	GTYRAC	1 cut(s)	1822
HindIII	AAGCTT	2 cut(s)	1022, 1199
HpaII	CCGG	9 cut(s)	51, 86, 175, 529, 1222, 1584, 1696, 1817, 2276
HphI	GGTGA	9 cut(s)	100, 401, 698, 737, 743, 893, 2192, 2702, 3172
Hpy166II	GTNNAC	9 cut(s)	572, 689, 884, 1581, 1814, 1822, 1858, 2557, 2693
Hpy8I	GTNNAC	9 cut(s)	572, 689, 884, 1581, 1814, 1822, 1858, 2557, 2693
Hpy99I	CGWCG	1 cut(s)	61
HpyAV	CCTTC	5 cut(s)	371, 379, 1086, 2255, 2639
HpyCH4III	ACNGT	5 cut(s)	279, 303, 1609, 2031, 2482
HpyCH4IV	ACGT	3 cut(s)	179, 2097, 2311
HpyF10VI	GCNNNNNNNGC	9 cut(s)	253, 910, 1030, 1039, 1669, 1753, 2468, 2897, 3146
HpySE526I	ACGT	3 cut(s)	179, 2097, 2311
HspAI	GCGC	2 cut(s)	247, 1835
KpnI	GGTACC	2 cut(s)	1359, 2917
Ksp22I	TGATCA	3 cut(s)	507, 1867, 2766
LguI	GCTCTTC	4 cut(s)	966, 1101, 1158, 3048
MaeI	CTAG	6 cut(s)	737, 848, 1227, 1998, 2417, 2778
MaeII	ACGT	3 cut(s)	179, 2097, 2311
MaeIII	GTNAC	7 cut(s)	145, 1253, 1455, 2164, 2180, 2616, 3178
MbiI	CCGCTC	1 cut(s)	325
MfeI	CAATTG	1 cut(s)	1878
MflI	RGATCY	4 cut(s)	637, 1465, 2602, 2773
MhlI	GDGCHC	8 cut(s)	607, 847, 1222, 2365, 2749, 2861, 2902, 3113
MlsI	TGGCCA	2 cut(s)	913, 930
MluNI	TGGCCA	2 cut(s)	913, 930
MlyI	GAGTC	5 cut(s)	170, 415, 551, 2081, 3001
MmeI	TCCRAC	5 cut(s)	143, 144, 261, 1925, 3167
Mox20I	TGGCCA	2 cut(s)	913, 930
MroXI	GAANNNNTTC	2 cut(s)	448, 3046
MscI	TGGCCA	2 cut(s)	913, 930
MslI	CAYNNNNRTG	2 cut(s)	2954, 3023
Msp20I	TGGCCA	2 cut(s)	913, 930
MspA1I	CMGCKG	2 cut(s)	750, 3149
MspCI	CTTAAG	1 cut(s)	2090
MspI	CCGG	9 cut(s)	51, 86, 175, 529, 1222, 1584, 1696, 1817, 2276
MspR9I	CCNGG	8 cut(s)	52, 226, 530, 621, 1222, 1223, 1284, 2565
MunI	CAATTG	1 cut(s)	1878
Mva1269I	GAATGC	2 cut(s)	1514, 2874
MvaI	CCWGG	4 cut(s)	226, 621, 1284, 2565
MvnI	CGCG	2 cut(s)	786, 2866
MwoI	GCNNNNNNNGC	9 cut(s)	253, 910, 1030, 1039, 1669, 1753, 2468, 2897, 3146
NciI	CCSGG	4 cut(s)	52, 530, 1222, 1223
NcoI	CCATGG	1 cut(s)	1246
NdeI	CATATG	1 cut(s)	916
NlaIV	GGNNCC	6 cut(s)	818, 1357, 1746, 1860, 2775, 2915
NmuCI	GTSAC	5 cut(s)	1455, 2164, 2180, 2616, 3178
NruI	TCGCGA	1 cut(s)	786
NspI	RCATGY	4 cut(s)	241, 361, 1261, 2953
PaeR7I	CTCGAG	2 cut(s)	230, 2895
PagI	TCATGA	2 cut(s)	1864, 3024
PciI	ACATGT	3 cut(s)	237, 357, 2949
PciSI	GCTCTTC	4 cut(s)	966, 1101, 1158, 3048
PcsI	WCGNNNNNNNCGW	1 cut(s)	208
PctI	GAATGC	2 cut(s)	1514, 2874
PdmI	GAANNNNTTC	2 cut(s)	448, 3046
PfeI	GAWTC	7 cut(s)	91, 1416, 2084, 2153, 2237, 2380, 2420
Pfl23II	CGTACG	1 cut(s)	2923
PflMI	CCANNNNNTGG	2 cut(s)	445, 2240
PinAI	ACCGGT	1 cut(s)	1816
Ple19I	CGATCG	1 cut(s)	66
PleI	GAGTC	5 cut(s)	169, 415, 551, 2080, 3001
PpsI	GAGTC	5 cut(s)	169, 415, 551, 2080, 3001
PpuMI	RGGWCCY	1 cut(s)	713
PscI	ACATGT	3 cut(s)	237, 357, 2949
PshBI	ATTAAT	1 cut(s)	1068
Psp124BI	GAGCTC	2 cut(s)	2365, 2861
Psp5II	RGGWCCY	1 cut(s)	713
Psp6I	CCWGG	4 cut(s)	224, 619, 1282, 2563
PspEI	GGTNACC	1 cut(s)	2180
PspGI	CCWGG	4 cut(s)	224, 619, 1282, 2563
PspLI	CGTACG	1 cut(s)	2923
PspN4I	GGNNCC	6 cut(s)	818, 1357, 1746, 1860, 2775, 2915
PspPI	GGNCC	8 cut(s)	713, 779, 816, 1309, 1814, 1858, 2243, 2681
PspPPI	RGGWCCY	1 cut(s)	713
PstI	CTGCAG	4 cut(s)	712, 1129, 2173, 2855
PstNI	CAGNNNCTG	3 cut(s)	716, 2177, 2240
PsuI	RGATCY	4 cut(s)	637, 1465, 2602, 2773
PvuI	CGATCG	1 cut(s)	66
PvuII	CAGCTG	1 cut(s)	3149
RruI	TCGCGA	1 cut(s)	786
RsaI	GTAC	9 cut(s)	173, 810, 1147, 1357, 2508, 2569, 2849, 2915, 2925
RsaNI	GTAC	9 cut(s)	172, 809, 1146, 1356, 2507, 2568, 2848, 2914, 2924
RseI	CAYNNNNRTG	2 cut(s)	2954, 3023
SacI	GAGCTC	2 cut(s)	2365, 2861
SapI	GCTCTTC	4 cut(s)	966, 1101, 1158, 3048
Sau96I	GGNCC	8 cut(s)	713, 779, 816, 1309, 1814, 1858, 2243, 2681
ScaI	AGTACT	1 cut(s)	810
SchI	GAGTC	5 cut(s)	170, 415, 551, 2081, 3001
ScrFI	CCNGG	8 cut(s)	52, 226, 530, 621, 1222, 1223, 1284, 2565
SduI	GDGCHC	8 cut(s)	607, 847, 1222, 2365, 2749, 2861, 2902, 3113
SfcI	CTRYAG	5 cut(s)	708, 1125, 1605, 2169, 2851
Sfr274I	CTCGAG	2 cut(s)	230, 2895
SinI	GGWCC	6 cut(s)	713, 779, 1309, 1814, 1858, 2243
SlaI	CTCGAG	2 cut(s)	230, 2895
SmaI	CCCGGG	1 cut(s)	1223
SmiMI	CAYNNNNRTG	2 cut(s)	2954, 3023
SmlI	CTYRAG	4 cut(s)	230, 1202, 2090, 2895
SmoI	CTYRAG	4 cut(s)	230, 1202, 2090, 2895
SrfI	GCCCGGGC	1 cut(s)	1223
SsiI	CCGC	7 cut(s)	5, 313, 325, 750, 1103, 2014, 2824
SspI	AATATT	1 cut(s)	2400
SspMI	CTAG	6 cut(s)	737, 848, 1227, 1998, 2417, 2778
SstI	GAGCTC	2 cut(s)	2365, 2861
StyD4I	CCNGG	8 cut(s)	50, 224, 528, 619, 1220, 1221, 1282, 2563
StyI	CCWWGG	3 cut(s)	1246, 1262, 2962
TaaI	ACNGT	5 cut(s)	279, 303, 1609, 2031, 2482
TaiI	ACGT	3 cut(s)	182, 2100, 2314
TaqI	TCGA	7 cut(s)	66, 231, 553, 1161, 2340, 2711, 2896
TatI	WGTACW	3 cut(s)	808, 1145, 2847
TauI	GCSGC	1 cut(s)	2016
TfiI	GAWTC	7 cut(s)	91, 1416, 2084, 2153, 2237, 2380, 2420
TseFI	GTSAC	5 cut(s)	1455, 2164, 2180, 2616, 3178
Tsp45I	GTSAC	5 cut(s)	1455, 2164, 2180, 2616, 3178
TspDTI	ATGAA	8 cut(s)	914, 1714, 2070, 2166, 2271, 2748, 3003, 3041
TspGWI	ACGGA	2 cut(s)	352, 2710
TspMI	CCCGGG	1 cut(s)	1221
Van91I	CCANNNNNTGG	2 cut(s)	445, 2240
Vha464I	CTTAAG	1 cut(s)	2090
VpaK11BI	GGWCC	6 cut(s)	713, 779, 1309, 1814, 1858, 2243
VspI	ATTAAT	1 cut(s)	1068
XapI	RAATTY	4 cut(s)	768, 830, 1915, 2698
XceI	RCATGY	4 cut(s)	241, 361, 1261, 2953
XcmI	CCANNNNNNNNNTGG	2 cut(s)	672, 1349
XhoI	CTCGAG	2 cut(s)	230, 2895
XmaI	CCCGGG	1 cut(s)	1221
XmiI	GTMKAC	1 cut(s)	1580
XmnI	GAANNNNTTC	2 cut(s)	448, 3046
XspI	CTAG	6 cut(s)	737, 848, 1227, 1998, 2417, 2778
ZrmI	AGTACT	1 cut(s)	810

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.