RchiOBHm_Chr4g0425801
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0014718
Physical Location & Seq
Forward (+)
51412786 .. 51414722
1937 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39491

Sequence Viewer

Length: 1167 bp
ATGGATGGTAGACAGGAGCTGAGAAACAACACCACCACCACCAATCGTACTACTCTTCCTCCTTCCTCCTCCTCCTCTGCACCGATGGCCAACTCAGCACTTTTCTCCGATCACGAGATTCCGATGATACCCAACAGCACCTTCTCTTCACTCTCAAGCATCTTCGACTCAGAACCCGAACGGGGTTATCTGGGTTTCATGGAGTTGCTCGGCAACACGGACTTCGCTCCTGTGGTTGATTTCGGGACCCAAACGACGCCGTCTGACTCGTTGGTGACGATGATGATGCCACCTCAGCAGCAGCAGCAGCAAACACAAGCGCTTCCGTCGCCGGTGTCGTCTACAGTGCCAGAGAGTGGGACTAACTCCGAGGCCTTGAACTCGGGCGCCAATACTGCTCCCACGACACCCAACTCGTCTTCGATCTCGTCCTCGTCTAATGAAGCTCATGCGGCGAATAATAATAATGAGGAGCAAACCACGACAAAAGCAGGAGATGAGGAAGAAGATGCTGACCAGGATCCGGACAAGACCCAGAAACAATTGAAACCCAAAAAGAAGAACCAGAAAAGGCAGAGGGAGCCGAGATTTGCGTTCATGACCAAGAGCGAGGTCGATAACCTAGACGACGGGTACAGATGGCGCAAGTACGGCCAAAAAGCTGTCAAGAACAGCCCTTATCCAAGGAGCTACTATCGTTGCACCACAGCAGGTTGCGGTGTGAAGAAGAGGGTGGAGAGGTCCTCCGACGACCCTTCGATTGTGGTGACGACTTACGAAGGCCAGCACACGCACCCGAGCCCGATCACGCCGCGCGGCAGCATGGGGATGGCGCCGCTGCCGTCGGAGCTGTCCGGTCATTTGTTCACCGGAGCGGGCTCAGGCTATGGTGTTCCTCATCACTATCAACATCAGCAGCAACAGCAACAGCATTTGCACTCTTATATATATAACTCTTCGCCTTCTTTGAACATTACTACTACCACCTCCTCAGCGCCATCCTATGGTTTCAACCCGGCGTCGTGTCATGGTTTGTTTCAACAGAGATATAATAACACTGGAGGAGGTCATTCCTCTAATTTGGCCGCTTCTAATTTGCTTAGAGACCACGGACTTCTTCAGGATATGCTGCCATCGCAGATTCGAAAAGAGACTAAGGAAGGGTAG

Protein Analysis

388

Amino Acids

42.4

Weight (kDa)

6.23

Isoelectric Point (pI)

57.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 209 - 266 2e-28 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014718)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g20230
malus_domestica MD13G1150700.v1.1 MD16G1151000.v1.1
prunus_persica Prupe.1G114800_v2.0.a1
pyrus_communis pycom13g12950 pycom16g12920
rosa_chinensis RchiOBHm_Chr4g0425801
rosa_laevigata RLG00000007339
rosa_multiflora Rmu_sc0007063.1_g000001
rosa_roxburghii Rroxscaffold_5G00367790
rosa_rugosa Rorug04G0206200 Rorug04G0206300
rosa_samantha Rh4AG263500 Rh4BG269400 Rh4CG281800 Rh4DG265800
rosa_wichuraiana Rw4G022740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 701
AccB1I GGYRCC 2 cut(s) 386, 832
AccB7I CCANNNNNTGG 2 cut(s) 356, 1004
AccBSI CCGCTC 1 cut(s) 875
AccI GTMKAC 2 cut(s) 10, 341
AccII CGCG 2 cut(s) 814, 816
AccIII TCCGGA 1 cut(s) 523
AciI CCGC 7 cut(s) 452, 717, 812, 816, 836, 875, 1086
AclWI GGATC 2 cut(s) 515, 528
AcoI YGGCCR 3 cut(s) 87, 652, 1083
AcuI CTGAAG 1 cut(s) 1103
AcyI GRCGYC 4 cut(s) 257, 387, 833, 1019
AfaI GTAC 3 cut(s) 49, 635, 650
AfeI AGCGCT 1 cut(s) 321
AfiI CCNNNNNNNGG 4 cut(s) 182, 356, 523, 1004
AgsI TTSAA 5 cut(s) 379, 547, 970, 1012, 1040
AjnI CCWGG 1 cut(s) 516
AjuI GAANNNNNNNTTGG 2 cut(s) 125, 157
AluBI AGCT 5 cut(s) 19, 446, 662, 690, 850
AluI AGCT 5 cut(s) 19, 446, 662, 690, 850
Alw26I GTCTC 2 cut(s) 1098, 1145
AlwI GGATC 2 cut(s) 515, 528
AlwNI CAGNNNCTG 1 cut(s) 19
Ama87I CYCGRG 2 cut(s) 382, 796
Aor13HI TCCGGA 1 cut(s) 523
Aor51HI AGCGCT 1 cut(s) 321
AoxI GGCC 5 cut(s) 87, 372, 652, 781, 1083
ApeKI GCWGC 8 cut(s) 298, 301, 304, 307, 819, 838, 916, 1129
AspLEI GCGC 6 cut(s) 322, 389, 645, 816, 835, 997
AspS9I GGNCC 2 cut(s) 246, 741
AsuC2I CCSGG 1 cut(s) 1016
AsuHPI GGTGA 3 cut(s) 286, 778, 859
AsuII TTCGAA 1 cut(s) 1144
AvaI CYCGRG 2 cut(s) 382, 796
AvaII GGWCC 2 cut(s) 246, 741
BalI TGGCCA 1 cut(s) 89
BamHI GGATCC 1 cut(s) 520
BanI GGYRCC 2 cut(s) 386, 832
BanII GRGCYC 2 cut(s) 803, 881
BauI CACGAG 1 cut(s) 113
BbsI GAAGAC 1 cut(s) 411
BbvCI CCTCAGC 2 cut(s) 294, 991
BbvI GCAGC 8 cut(s) 310, 313, 316, 319, 825, 831, 928, 1116
BccI CCATC 5 cut(s) 79, 633, 823, 1006, 1141
BceAI ACGGC 3 cut(s) 244, 667, 826
BcgI CGANNNNNNTGC 2 cut(s) 268, 302
BciT130I CCWGG 1 cut(s) 518
BcnI CCSGG 1 cut(s) 1016
BcoDI GTCTC 2 cut(s) 1098, 1145
BfaI CTAG 1 cut(s) 623
BfmI CTRYAG 1 cut(s) 342
BfoI RGCGCY 4 cut(s) 323, 390, 836, 998
BfuAI ACCTGC 1 cut(s) 701
Bme1390I CCNGG 2 cut(s) 518, 1016
Bme18I GGWCC 2 cut(s) 246, 741
BmeT110I CYCGRG 2 cut(s) 382, 796
BmgT120I GGNCC 2 cut(s) 246, 741
BmiI GGNNCC 6 cut(s) 247, 248, 388, 522, 582, 834
BmrFI CCNGG 2 cut(s) 518, 1016
BmsI GCATC 3 cut(s) 168, 276, 499
BpiI GAAGAC 1 cut(s) 411
BplI GAGNNNNNCTC 2 cut(s) 728, 760
BpmI CTGGAG 1 cut(s) 1080
Bpu10I CCTNAGC 3 cut(s) 294, 880, 991
Bpu14I TTCGAA 1 cut(s) 1144
BpuEI CTTGAG 1 cut(s) 139
BpuMI CCSGG 1 cut(s) 1016
BsaHI GRCGYC 4 cut(s) 257, 387, 833, 1019
BsaI GGTCTC 1 cut(s) 1098
BsaJI CCNNGG 3 cut(s) 369, 683, 1108
BsaWI WCCGGW 3 cut(s) 523, 854, 869
BsaXI ACNNNNNCTCC 2 cut(s) 43, 73
Bsc4I CCNNNNNNNGG 4 cut(s) 182, 356, 523, 1004
Bse118I RCCGGY 1 cut(s) 331
Bse1I ACTGG 1 cut(s) 1063
BseAI TCCGGA 1 cut(s) 523
BseBI CCWGG 1 cut(s) 518
BseDI CCNNGG 3 cut(s) 369, 683, 1108
BseGI GGATG 3 cut(s) 10, 834, 998
BseLI CCNNNNNNNGG 4 cut(s) 182, 356, 523, 1004
BseMII CTCAG 6 cut(s) 11, 108, 183, 308, 894, 1005
BseNI ACTGG 1 cut(s) 1063
BseRI GAGGAG 6 cut(s) 58, 61, 64, 485, 979, 1077
BseXI GCAGC 8 cut(s) 310, 313, 316, 319, 825, 831, 928, 1116
BsgI GTGCAG 1 cut(s) 63
Bsh1236I CGCG 2 cut(s) 814, 816
BshFI GGCC 5 cut(s) 89, 374, 654, 783, 1085
BshNI GGYRCC 2 cut(s) 386, 832
BsiHKCI CYCGRG 2 cut(s) 382, 796
BsiSI CCGG 5 cut(s) 332, 524, 855, 870, 1016
BslFI GGGAC 2 cut(s) 259, 373
BslI CCNNNNNNNGG 4 cut(s) 182, 356, 523, 1004
BsmAI GTCTC 2 cut(s) 1098, 1145
BsmFI GGGAC 2 cut(s) 259, 373
BsnI GGCC 5 cut(s) 89, 374, 654, 783, 1085
Bso31I GGTCTC 1 cut(s) 1098
BsoBI CYCGRG 2 cut(s) 382, 796
Bsp119I TTCGAA 1 cut(s) 1144
Bsp1286I GDGCHC 2 cut(s) 803, 881
Bsp13I TCCGGA 1 cut(s) 523
Bsp143I GATC 4 cut(s) 109, 423, 520, 804
BspACI CCGC 7 cut(s) 452, 717, 812, 816, 836, 875, 1086
BspANI GGCC 5 cut(s) 89, 374, 654, 783, 1085
BspCNI CTCAG 6 cut(s) 12, 107, 182, 307, 893, 1004
BspEI TCCGGA 1 cut(s) 523
BspFNI CGCG 2 cut(s) 814, 816
BspHI TCATGA 1 cut(s) 597
BspLI GGNNCC 6 cut(s) 247, 248, 388, 522, 582, 834
BspMI ACCTGC 1 cut(s) 701
BspPI GGATC 2 cut(s) 515, 528
BspT104I TTCGAA 1 cut(s) 1144
BspT107I GGYRCC 2 cut(s) 386, 832
BspTNI GGTCTC 1 cut(s) 1098
BsrBI CCGCTC 1 cut(s) 875
BsrFI RCCGGY 1 cut(s) 331
BsrI ACTGG 1 cut(s) 1063
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 3 cut(s) 369, 683, 1108
BssMI GATC 4 cut(s) 109, 423, 520, 804
BssNI GRCGYC 4 cut(s) 257, 387, 833, 1019
BssSI CACGAG 1 cut(s) 113
BssT1I CCWWGG 1 cut(s) 683
Bst2BI CACGAG 1 cut(s) 113
Bst2UI CCWGG 1 cut(s) 518
Bst4CI ACNGT 1 cut(s) 346
Bst6I CTCTTC 4 cut(s) 60, 151, 722, 961
BstACI GRCGYC 4 cut(s) 257, 387, 833, 1019
BstBI TTCGAA 1 cut(s) 1144
BstC8I GCNNGC 2 cut(s) 785, 877
BstDEI CTNAG 8 cut(s) 20, 94, 169, 294, 880, 991, 1100, 1155
BstDSI CCRYGG 1 cut(s) 1108
BstF5I GGATG 3 cut(s) 10, 834, 998
BstFNI CGCG 2 cut(s) 814, 816
BstH2I RGCGCY 4 cut(s) 323, 390, 836, 998
BstHHI GCGC 6 cut(s) 322, 389, 645, 816, 835, 997
BstKTI GATC 4 cut(s) 112, 426, 523, 807
BstMAI GTCTC 2 cut(s) 1098, 1145
BstMBI GATC 4 cut(s) 109, 423, 520, 804
BstNI CCWGG 1 cut(s) 518
BstSCI CCNGG 2 cut(s) 516, 1014
BstSFI CTRYAG 1 cut(s) 342
BstUI CGCG 2 cut(s) 814, 816
BstV1I GCAGC 8 cut(s) 310, 313, 316, 319, 825, 831, 928, 1116
BstV2I GAAGAC 1 cut(s) 411
BstX2I RGATCY 1 cut(s) 520
BstYI RGATCY 1 cut(s) 520
BsuRI GGCC 5 cut(s) 89, 374, 654, 783, 1085
BtgI CCRYGG 1 cut(s) 1108
BtgZI GCGATG 1 cut(s) 1119
BtsCI GGATG 3 cut(s) 10, 834, 998
BtsIMutI CAGTG 2 cut(s) 351, 1056
BveI ACCTGC 1 cut(s) 701
Cac8I GCNNGC 2 cut(s) 785, 877
CaiI CAGNNNCTG 1 cut(s) 19
CciI TCATGA 1 cut(s) 597
CfoI GCGC 6 cut(s) 322, 389, 645, 816, 835, 997
Cfr10I RCCGGY 1 cut(s) 331
Cfr13I GGNCC 2 cut(s) 246, 741
CseI GACGC 2 cut(s) 265, 1008
Csp6I GTAC 3 cut(s) 48, 634, 649
CviAII CATG 5 cut(s) 199, 449, 598, 823, 1028
CviQI GTAC 3 cut(s) 48, 634, 649
DdeI CTNAG 8 cut(s) 20, 94, 169, 294, 880, 991, 1100, 1155
DinI GGCGCC 2 cut(s) 388, 834
DpnI GATC 4 cut(s) 111, 425, 522, 806
DpnII GATC 4 cut(s) 109, 423, 520, 804
EaeI YGGCCR 3 cut(s) 87, 652, 1083
Eam1104I CTCTTC 4 cut(s) 60, 151, 722, 961
EarI CTCTTC 4 cut(s) 60, 151, 722, 961
Eco130I CCWWGG 1 cut(s) 683
Eco147I AGGCCT 1 cut(s) 374
Eco24I GRGCYC 2 cut(s) 803, 881
Eco31I GGTCTC 1 cut(s) 1098
Eco47I GGWCC 2 cut(s) 246, 741
Eco47III AGCGCT 1 cut(s) 321
Eco57I CTGAAG 1 cut(s) 1103
Eco88I CYCGRG 2 cut(s) 382, 796
EcoO109I RGGNCCY 2 cut(s) 246, 741
EcoRII CCWGG 1 cut(s) 516
EcoT14I CCWWGG 1 cut(s) 683
EcoT38I GRGCYC 2 cut(s) 803, 881
EgeI GGCGCC 2 cut(s) 388, 834
EheI GGCGCC 2 cut(s) 388, 834
ErhI CCWWGG 1 cut(s) 683
FaeI CATG 5 cut(s) 202, 452, 601, 826, 1031
FaqI GGGAC 2 cut(s) 259, 373
FatI CATG 5 cut(s) 198, 448, 597, 822, 1027
FauI CCCGC 1 cut(s) 868
FblI GTMKAC 2 cut(s) 10, 341
FokI GGATG 3 cut(s) 17, 841, 985
FriOI GRGCYC 2 cut(s) 803, 881
FspBI CTAG 1 cut(s) 623
GlaI GCGC 6 cut(s) 321, 388, 644, 815, 834, 996
GsuI CTGGAG 1 cut(s) 1080
HaeII RGCGCY 4 cut(s) 323, 390, 836, 998
HaeIII GGCC 5 cut(s) 89, 374, 654, 783, 1085
HapII CCGG 5 cut(s) 332, 524, 855, 870, 1016
HgaI GACGC 2 cut(s) 265, 1008
HhaI GCGC 6 cut(s) 322, 389, 645, 816, 835, 997
Hin1I GRCGYC 4 cut(s) 257, 387, 833, 1019
Hin1II CATG 5 cut(s) 202, 452, 601, 826, 1031
Hin6I GCGC 6 cut(s) 320, 387, 643, 814, 833, 995
HinP1I GCGC 6 cut(s) 320, 387, 643, 814, 833, 995
HinfI GANTC 4 cut(s) 118, 167, 266, 1141
HpaII CCGG 5 cut(s) 332, 524, 855, 870, 1016
HphI GGTGA 3 cut(s) 286, 778, 859
Hpy166II GTNNAC 3 cut(s) 11, 342, 867
Hpy188I TCNGA 7 cut(s) 109, 123, 172, 265, 370, 748, 847
Hpy188III TCNNGA 6 cut(s) 113, 244, 524, 598, 667, 1121
Hpy8I GTNNAC 3 cut(s) 11, 342, 867
Hpy99I CGWCG 6 cut(s) 259, 331, 632, 752, 847, 1024
HpyAV CCTTC 6 cut(s) 72, 151, 765, 773, 972, 1154
HpyCH4III ACNGT 1 cut(s) 346
HpyCH4V TGCA 3 cut(s) 80, 702, 937
HpyF3I CTNAG 8 cut(s) 20, 94, 169, 294, 880, 991, 1100, 1155
Hsp92I GRCGYC 4 cut(s) 257, 387, 833, 1019
Hsp92II CATG 5 cut(s) 202, 452, 601, 826, 1031
HspAI GCGC 6 cut(s) 320, 387, 643, 814, 833, 995
KasI GGCGCC 2 cut(s) 386, 832
KflI GGGWCCC 1 cut(s) 246
Kpn2I TCCGGA 1 cut(s) 523
Kzo9I GATC 4 cut(s) 109, 423, 520, 804
LmnI GCTCC 8 cut(s) 16, 232, 403, 472, 580, 687, 847, 872
Lsp1109I GCAGC 8 cut(s) 310, 313, 316, 319, 825, 831, 928, 1116
LweI GCATC 3 cut(s) 168, 276, 499
MaeI CTAG 1 cut(s) 623
MaeIII GTNAC 2 cut(s) 274, 766
MalI GATC 4 cut(s) 111, 425, 522, 806
MbiI CCGCTC 1 cut(s) 875
MboI GATC 4 cut(s) 109, 423, 520, 804
MfeI CAATTG 1 cut(s) 542
MflI RGATCY 1 cut(s) 520
MhlI GDGCHC 2 cut(s) 803, 881
MlsI TGGCCA 1 cut(s) 89
MluCI AATT 3 cut(s) 542, 1078, 1093
MluNI TGGCCA 1 cut(s) 89
Mly113I GGCGCC 2 cut(s) 387, 833
MlyI GAGTC 2 cut(s) 161, 260
MmeI TCCRAC 2 cut(s) 771, 825
Mox20I TGGCCA 1 cut(s) 89
MroI TCCGGA 1 cut(s) 523
MscI TGGCCA 1 cut(s) 89
MslI CAYNNNNRTG 1 cut(s) 827
Msp20I TGGCCA 1 cut(s) 89
MspA1I CMGCKG 1 cut(s) 838
MspI CCGG 5 cut(s) 332, 524, 855, 870, 1016
MspR9I CCNGG 2 cut(s) 518, 1016
MunI CAATTG 1 cut(s) 542
MvaI CCWGG 1 cut(s) 518
MvnI CGCG 2 cut(s) 814, 816
NarI GGCGCC 2 cut(s) 387, 833
NciI CCSGG 1 cut(s) 1016
NdeII GATC 4 cut(s) 109, 423, 520, 804
NlaIII CATG 5 cut(s) 202, 452, 601, 826, 1031
NlaIV GGNNCC 6 cut(s) 247, 248, 388, 522, 582, 834
NmeAIII GCCGAG 2 cut(s) 189, 609
NmuCI GTSAC 2 cut(s) 274, 766
NspV TTCGAA 1 cut(s) 1144
PagI TCATGA 1 cut(s) 597
PceI AGGCCT 1 cut(s) 374
PcsI WCGNNNNNNNCGW 2 cut(s) 275, 335
PfeI GAWTC 2 cut(s) 118, 1141
PflFI GACNNNGTC 1 cut(s) 259
PflMI CCANNNNNTGG 2 cut(s) 356, 1004
PleI GAGTC 2 cut(s) 161, 260
PluTI GGCGCC 2 cut(s) 390, 836
PpsI GAGTC 2 cut(s) 161, 260
PpuMI RGGWCCY 2 cut(s) 246, 741
Psp5II RGGWCCY 2 cut(s) 246, 741
Psp6I CCWGG 1 cut(s) 516
PspGI CCWGG 1 cut(s) 516
PspN4I GGNNCC 6 cut(s) 247, 248, 388, 522, 582, 834
PspPI GGNCC 2 cut(s) 246, 741
PspPPI RGGWCCY 2 cut(s) 246, 741
PstNI CAGNNNCTG 1 cut(s) 19
PsuI RGATCY 1 cut(s) 520
PsyI GACNNNGTC 1 cut(s) 259
RsaI GTAC 3 cut(s) 49, 635, 650
RsaNI GTAC 3 cut(s) 48, 634, 649
RseI CAYNNNNRTG 1 cut(s) 827
Sau3AI GATC 4 cut(s) 109, 423, 520, 804
Sau96I GGNCC 2 cut(s) 246, 741
SchI GAGTC 2 cut(s) 161, 260
ScrFI CCNGG 2 cut(s) 518, 1016
SduI GDGCHC 2 cut(s) 803, 881
SfaNI GCATC 3 cut(s) 168, 276, 499
SfcI CTRYAG 1 cut(s) 342
SfoI GGCGCC 2 cut(s) 388, 834
SfuI TTCGAA 1 cut(s) 1144
SgrAI CRCCGGYG 1 cut(s) 331
SinI GGWCC 2 cut(s) 246, 741
SmiMI CAYNNNNRTG 1 cut(s) 827
SmlI CTYRAG 1 cut(s) 154
SmoI CTYRAG 1 cut(s) 154
Sse9I AATT 3 cut(s) 542, 1078, 1093
SseBI AGGCCT 1 cut(s) 374
SsiI CCGC 7 cut(s) 452, 717, 812, 816, 836, 875, 1086
SspDI GGCGCC 2 cut(s) 386, 832
SspMI CTAG 1 cut(s) 623
StuI AGGCCT 1 cut(s) 374
StyD4I CCNGG 2 cut(s) 516, 1014
StyI CCWWGG 1 cut(s) 683
TaaI ACNGT 1 cut(s) 346
TaqI TCGA 5 cut(s) 165, 422, 615, 758, 1144
TasI AATT 3 cut(s) 542, 1078, 1093
TauI GCSGC 5 cut(s) 455, 814, 819, 838, 1088
TfiI GAWTC 2 cut(s) 118, 1141
TscAI CASTG 2 cut(s) 351, 1063
TseFI GTSAC 2 cut(s) 274, 766
TseI GCWGC 8 cut(s) 298, 301, 304, 307, 819, 838, 916, 1129
Tsp45I GTSAC 2 cut(s) 274, 766
TspDTI ATGAA 3 cut(s) 187, 456, 586
TspGWI ACGGA 3 cut(s) 233, 315, 1125
TspRI CASTG 2 cut(s) 351, 1063
Tth111I GACNNNGTC 1 cut(s) 259
Van91I CCANNNNNTGG 2 cut(s) 356, 1004
VpaK11BI GGWCC 2 cut(s) 246, 741
XmiI GTMKAC 2 cut(s) 10, 341
XspI CTAG 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.