MD13G1158900.v1.1

Belongs to the importin alpha family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008963
Physical Location & Seq
Forward (+)
12502305 .. 12506536
4232 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1158900.v1.1.491

Sequence Viewer

Length: 1587 bp
ATGTCGCTGAGGCCGAGCGCGAGGACCGAGGTCCGCCGGAACCGCTACAAGGTGGCGGTGGACGCCGAGGAGGGACGTCGCAGGAGAGAGGACAACATGGTGGAGATCCGGAAGAGCAAGCGCGAAGAGAGCTTGCTGAAGAAGCGTCGCGAAGGCCTCCAAGCGCAACAATTCGCGCCATCTCTCCAGCCATCAAATCTCGAGAAGAAGTTAGAGAGTCTACCTGCCATGGTTGCTGGGGTTTGGTCTGACGACAACAGTTTGCAGCTGGAGTCCACTACTCAGTTCCGAAAACTGCTTTCGATTGAACGAAGCCCTCCGATTGAGGAAGTTATCCAATCAGGAGTTGTTCCCCGCTTTGTGGCATTCCTCATGAGGGAGGATTTTCCGCAACTTCAGTTCGAGGCTGCTTGGGCTCTTACAAACATAGCGTCAGGAACCTCAGAGAACACCAAGGTGGTGATTGAGCAGGGGGCAGTCCCAATCTTTGTAAAGCTTCTTGCTTCTCCGAGTGATGATGTTAGGGAGCAGGCTGTGTGGGCATTGGGAAATGTTGCTGGTGATTCCCCTAGATGTCGTGATCTTGTCCTCAGCAGTGGAGCCTTGATGCCGTTGCTTGCTCAGTTGAATGAGCATGCAAAGCTTTCAATGCTGAGAAATGCAACATGGACACTGTCTAACTTTTGCAGGGGCAAGCCACAGCCTCCATTTGACCAGGTGAAGCCAGCACTTCCAGCTCTTGAACGTTTAGTTCATTCCAATGATGAAGAAGTCTTGACAGATGCCTGCTGGGCACTCTCGTATCTCTCGGATGGCACGAATGACAAAATCCAAGCTGTGATTGAGGCAGGTGTCTGTGTCCGACTTGTGCAGCTCCTCTTACATCCATCTCCTTCTGTTCTCATTCCTGCACTTCGCACTGTTGGTAATATTGTGACTGGAGATGATCTTCAGACTCAGTGTATCATCAACAATGGTTCACTCCCATGCCTTCTGAGCCTGCTGACTCATAATCATAAAAAGAGCATCAAGAAAGAAGCTTGCTGGACAATCTCAAACATTACAGCTGGAAACAGGGATCAAATACAGTCTATAATTGAAGCTGGTTTGATTGGTCCTCTGGTCAACTTACTCCAACATGCTGAGTTTGACATAAAGAAAGAAGCTGCATGGGCAATCTCAAATGCTACTTCTGGTGGCACTCCTGATCAAATCAAGTACCTGGTTGGCGAAGGCTGTATAAAACCATTGTGTGATCTTCTTGTATGCCCTGATCCAAGGATCGTCACCGTCTGTTTAGAGGGGTTGGAGAACATTCTGAAGGTTGGGGAAGCTGAAAAAGCTGCGGGTAACAGTGAAGTTAATTTGTATGCCCAGCTGGTTGATGATGCTGAGGGTCTAGAAAAGATTGAAAATCTACAGAGTCACGACAACACTGAGATCTATGAGAAGTCTGTAAAAATTCTGGAGACATATTGGTTGGAGGAAGACGAAGAGACATTACCAGCAGGAGACGGATCCCAGCCAGGCTTCCGCTTTGGAGGGAGTGAGGTTCAGGTTCCATCTGGTGGATTCAACTTTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm Arm_3 IBB

Protein Analysis

529

Amino Acids

58.12

Weight (kDa)

5.13

Isoelectric Point (pI)

54.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IBB PF01749 12 - 94 1.8e-23 Importin beta binding domain
Arm PF00514 105 - 145 1.4e-10 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 108 - 230 1e-05 ARMC5
HEAT_EZ PF13513 130 - 185 3.1e-10 HEAT-like repeat
Arm PF00514 147 - 186 1.1e-12 Armadillo/beta-catenin-like repeat
HEAT_2 PF13646 161 - 267 1.8e-06 HEAT repeats
Arm PF00514 189 - 230 2.7e-07 Armadillo/beta-catenin-like repeat
Arm PF00514 243 - 271 3.9e-07 Armadillo/beta-catenin-like repeat
Arm PF00514 274 - 312 3.3e-08 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 308 - 489 5.3e-08 ARMC5
Arm PF00514 316 - 356 1.5e-09 Armadillo/beta-catenin-like repeat
Arm PF00514 358 - 397 1.3e-11 Armadillo/beta-catenin-like repeat
Arm PF00514 401 - 440 1.4e-07 Armadillo/beta-catenin-like repeat
Arm_3 PF16186 455 - 499 1.3e-19 Atypical Arm repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0008963)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32880 AT3G06720 AT3G06720 AT4G16143 AT4G16143
fragaria_vesca FvH4_4g19270 FvH4_4g19270
malus_domestica MD13G1158900.v1.1 MD16G1159100.v1.1
prunus_persica Prupe.1G125900_v2.0.a1
pyrus_communis pycom16g13410
rosa_chinensis RchiOBHm_Chr4g0424261
rosa_laevigata RLG00000007492
rosa_multiflora Rmu_sc0006403.1_g000001
rosa_roxburghii Rroxscaffold_5G00366200
rosa_rugosa Rorug04G0194700 Rorug04G0194800
rosa_samantha Rh4AG252100 Rh4BG257500 Rh4CG268200 Rh4DG251900
rosa_wichuraiana Rw4G021740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 839
AatII GACGTC 1 cut(s) 79
Acc36I ACCTGC 2 cut(s) 232, 839
AccB7I CCANNNNNTGG 1 cut(s) 1568
AccI GTMKAC 1 cut(s) 220
AccII CGCG 4 cut(s) 20, 123, 150, 176
AccIII TCCGGA 1 cut(s) 108
AciI CCGC 7 cut(s) 34, 43, 56, 355, 389, 1346, 1534
AclI AACGTT 1 cut(s) 745
AclWI GGATC 6 cut(s) 100, 1086, 1268, 1289, 1512, 1525
AcsI RAATTY 1 cut(s) 1461
AcuI CTGAAG 4 cut(s) 158, 380, 935, 1340
AcyI GRCGYC 2 cut(s) 63, 76
AfaI GTAC 1 cut(s) 1220
AfiI CCNNNNNNNGG 4 cut(s) 49, 361, 376, 1568
AgsI TTSAA 7 cut(s) 308, 628, 648, 743, 1100, 1412, 1576
AjnI CCWGG 3 cut(s) 714, 1221, 1525
AleI CACNNNNGTG 1 cut(s) 455
Alw26I GTCTC 3 cut(s) 1463, 1490, 1506
AlwI GGATC 6 cut(s) 100, 1086, 1268, 1289, 1512, 1525
Ama87I CYCGRG 1 cut(s) 200
Aor13HI TCCGGA 1 cut(s) 108
AoxI GGCC 2 cut(s) 11, 154
ApeKI GCWGC 5 cut(s) 265, 407, 871, 1166, 1343
ApoI RAATTY 1 cut(s) 1461
ArsI GACNNNNNNTTYG 2 cut(s) 416, 448
AspLEI GCGC 4 cut(s) 20, 123, 166, 178
AspS9I GGNCC 3 cut(s) 24, 31, 1115
AsuHPI GGTGA 4 cut(s) 472, 572, 730, 1279
AvaI CYCGRG 1 cut(s) 200
AvaII GGWCC 3 cut(s) 24, 31, 1115
BaeGI GKGCMC 1 cut(s) 796
BaeI ACNNNNGTAYC 2 cut(s) 785, 818
BamHI GGATCC 1 cut(s) 1517
BanII GRGCYC 1 cut(s) 418
BarI GAAGNNNNNNTAC 2 cut(s) 1485, 1517
BbsI GAAGAC 1 cut(s) 1494
BbvCI CCTCAGC 3 cut(s) 8, 590, 1392
BbvI GCAGC 5 cut(s) 277, 394, 883, 1153, 1330
BccI CCATC 5 cut(s) 187, 199, 806, 895, 1570
BceAI ACGGC 1 cut(s) 595
BciT130I CCWGG 3 cut(s) 716, 1223, 1527
BclI TGATCA 1 cut(s) 1207
BcoDI GTCTC 3 cut(s) 1463, 1490, 1506
BfaI CTAG 2 cut(s) 570, 1400
BfmI CTRYAG 1 cut(s) 1418
BfuAI ACCTGC 2 cut(s) 232, 839
BglI GCCNNNNNGGC 1 cut(s) 791
BglII AGATCT 1 cut(s) 1440
BisI GCNGC 5 cut(s) 266, 408, 872, 1167, 1344
BlsI GCNGC 5 cut(s) 267, 409, 873, 1168, 1345
Bme1390I CCNGG 3 cut(s) 716, 1223, 1527
Bme18I GGWCC 3 cut(s) 24, 31, 1115
BmeT110I CYCGRG 1 cut(s) 200
BmgT120I GGNCC 3 cut(s) 24, 31, 1115
BmiI GGNNCC 5 cut(s) 41, 439, 601, 1519, 1560
BmrFI CCNGG 3 cut(s) 716, 1223, 1527
BmsI GCATC 4 cut(s) 597, 772, 1035, 1378
BoxI GACNNNNGTC 1 cut(s) 29
BpiI GAAGAC 1 cut(s) 1494
BpmI CTGGAG 4 cut(s) 170, 290, 960, 1487
Bpu10I CCTNAGC 3 cut(s) 8, 590, 1392
BsaHI GRCGYC 2 cut(s) 63, 76
BsaJI CCNNGG 5 cut(s) 27, 66, 228, 453, 1277
BsaWI WCCGGW 1 cut(s) 108
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 361, 376, 1568
Bse1I ACTGG 1 cut(s) 943
BseAI TCCGGA 1 cut(s) 108
BseBI CCWGG 3 cut(s) 716, 1223, 1527
BseDI CCNNGG 5 cut(s) 27, 66, 228, 453, 1277
BseGI GGATG 2 cut(s) 817, 883
BseLI CCNNNNNNNGG 4 cut(s) 49, 361, 376, 1568
BseNI ACTGG 1 cut(s) 943
BseRI GAGGAG 2 cut(s) 83, 866
BseSI GKGCMC 1 cut(s) 796
BseXI GCAGC 5 cut(s) 277, 394, 883, 1153, 1330
BseYI CCCAGC 4 cut(s) 236, 789, 1374, 1521
BsgI GTGCAG 2 cut(s) 890, 894
Bsh1236I CGCG 4 cut(s) 20, 123, 150, 176
BshFI GGCC 2 cut(s) 13, 156
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 2 cut(s) 37, 109
BslFI GGGAC 2 cut(s) 87, 464
BslI CCNNNNNNNGG 4 cut(s) 49, 361, 376, 1568
BsmAI GTCTC 3 cut(s) 1463, 1490, 1506
BsmBI CGTCTC 1 cut(s) 1506
BsmFI GGGAC 2 cut(s) 87, 464
BsmI GAATGC 1 cut(s) 365
BsnI GGCC 2 cut(s) 13, 156
BsoBI CYCGRG 1 cut(s) 200
Bsp1286I GDGCHC 2 cut(s) 418, 796
Bsp13I TCCGGA 1 cut(s) 108
Bsp19I CCATGG 1 cut(s) 228
Bsp68I TCGCGA 1 cut(s) 150
BspACI CCGC 7 cut(s) 34, 43, 56, 355, 389, 1346, 1534
BspANI GGCC 2 cut(s) 13, 156
BspEI TCCGGA 1 cut(s) 108
BspFNI CGCG 4 cut(s) 20, 123, 150, 176
BspHI TCATGA 1 cut(s) 372
BspLI GGNNCC 5 cut(s) 41, 439, 601, 1519, 1560
BspMI ACCTGC 2 cut(s) 232, 839
BspPI GGATC 6 cut(s) 100, 1086, 1268, 1289, 1512, 1525
BspQI GCTCTTC 1 cut(s) 107
BsrI ACTGG 1 cut(s) 943
BssECI CCNNGG 5 cut(s) 27, 66, 228, 453, 1277
BssNI GRCGYC 2 cut(s) 63, 76
BssT1I CCWWGG 3 cut(s) 228, 453, 1277
Bst2UI CCWGG 3 cut(s) 716, 1223, 1527
Bst4CI ACNGT 6 cut(s) 260, 675, 922, 1089, 1291, 1355
Bst6I CTCTTC 3 cut(s) 107, 120, 1488
BstACI GRCGYC 2 cut(s) 63, 76
BstDSI CCRYGG 1 cut(s) 228
BstF5I GGATG 2 cut(s) 817, 883
BstFNI CGCG 4 cut(s) 20, 123, 150, 176
BstHHI GCGC 4 cut(s) 20, 123, 166, 178
BstMAI GTCTC 3 cut(s) 1463, 1490, 1506
BstNI CCWGG 3 cut(s) 716, 1223, 1527
BstNSI RCATGY 2 cut(s) 638, 1142
BstPAI GACNNNNGTC 1 cut(s) 29
BstSCI CCNGG 3 cut(s) 714, 1221, 1525
BstSFI CTRYAG 1 cut(s) 1418
BstSLI GKGCMC 1 cut(s) 796
BstUI CGCG 4 cut(s) 20, 123, 150, 176
BstV1I GCAGC 5 cut(s) 277, 394, 883, 1153, 1330
BstV2I GAAGAC 1 cut(s) 1494
BstX2I RGATCY 3 cut(s) 105, 1440, 1517
BstYI RGATCY 3 cut(s) 105, 1440, 1517
BsuRI GGCC 2 cut(s) 13, 156
BtgI CCRYGG 1 cut(s) 228
BtsCI GGATG 2 cut(s) 817, 883
BtsI GCAGTG 1 cut(s) 601
BtsIMutI CAGTG 6 cut(s) 601, 671, 918, 965, 1360, 1434
BtuMI TCGCGA 1 cut(s) 150
BveI ACCTGC 2 cut(s) 232, 839
CciI TCATGA 1 cut(s) 372
CfoI GCGC 4 cut(s) 20, 123, 166, 178
Cfr13I GGNCC 3 cut(s) 24, 31, 1115
CseI GACGC 3 cut(s) 71, 134, 420
CsiI ACCWGGT 2 cut(s) 714, 1221
Csp6I GTAC 1 cut(s) 1219
CviAII CATG 8 cut(s) 97, 229, 373, 635, 666, 987, 1139, 1170
CviQI GTAC 1 cut(s) 1219
Eam1104I CTCTTC 3 cut(s) 107, 120, 1488
EarI CTCTTC 3 cut(s) 107, 120, 1488
EciI GGCGGA 1 cut(s) 23
Eco130I CCWWGG 3 cut(s) 228, 453, 1277
Eco147I AGGCCT 1 cut(s) 156
Eco24I GRGCYC 1 cut(s) 418
Eco47I GGWCC 3 cut(s) 24, 31, 1115
Eco57I CTGAAG 4 cut(s) 158, 380, 935, 1340
Eco88I CYCGRG 1 cut(s) 200
EcoRII CCWGG 3 cut(s) 714, 1221, 1525
EcoT14I CCWWGG 3 cut(s) 228, 453, 1277
EcoT38I GRGCYC 1 cut(s) 418
ErhI CCWWGG 3 cut(s) 228, 453, 1277
Esp3I CGTCTC 1 cut(s) 1506
FaeI CATG 8 cut(s) 100, 232, 376, 638, 669, 990, 1142, 1173
FaqI GGGAC 2 cut(s) 87, 464
FatI CATG 8 cut(s) 96, 228, 372, 634, 665, 986, 1138, 1169
FauI CCCGC 2 cut(s) 362, 1339
FbaI TGATCA 1 cut(s) 1207
FblI GTMKAC 1 cut(s) 220
Fnu4HI GCNGC 5 cut(s) 266, 408, 872, 1167, 1344
FokI GGATG 2 cut(s) 824, 870
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 5 cut(s) 266, 408, 872, 1167, 1344
FspBI CTAG 2 cut(s) 570, 1400
GlaI GCGC 4 cut(s) 19, 122, 165, 177
GluI GCNGC 5 cut(s) 266, 408, 872, 1167, 1344
GsaI CCCAGC 4 cut(s) 240, 793, 1378, 1525
GsuI CTGGAG 4 cut(s) 170, 290, 960, 1487
HaeIII GGCC 2 cut(s) 13, 156
HapII CCGG 2 cut(s) 37, 109
HgaI GACGC 3 cut(s) 71, 134, 420
HhaI GCGC 4 cut(s) 20, 123, 166, 178
Hin1I GRCGYC 2 cut(s) 63, 76
Hin1II CATG 8 cut(s) 100, 232, 376, 638, 669, 990, 1142, 1173
Hin6I GCGC 4 cut(s) 18, 121, 164, 176
HinP1I GCGC 4 cut(s) 18, 121, 164, 176
HincII GTYRAC 1 cut(s) 1126
HindII GTYRAC 1 cut(s) 1126
HindIII AAGCTT 3 cut(s) 494, 641, 1038
HinfI GANTC 7 cut(s) 217, 272, 563, 955, 1006, 1423, 1572
HpaII CCGG 2 cut(s) 37, 109
HphI GGTGA 4 cut(s) 472, 572, 730, 1279
Hpy166II GTNNAC 5 cut(s) 61, 221, 276, 980, 1126
Hpy8I GTNNAC 5 cut(s) 61, 221, 276, 980, 1126
Hpy99I CGWCG 2 cut(s) 81, 150
HpyAV CCTTC 5 cut(s) 146, 903, 1001, 1226, 1315
HpyCH4III ACNGT 6 cut(s) 260, 675, 922, 1089, 1291, 1355
HpyCH4IV ACGT 2 cut(s) 76, 745
HpyCH4V TGCA 7 cut(s) 265, 638, 662, 687, 871, 911, 1169
HpySE526I ACGT 2 cut(s) 76, 745
Hsp92I GRCGYC 2 cut(s) 63, 76
Hsp92II CATG 8 cut(s) 100, 232, 376, 638, 669, 990, 1142, 1173
HspAI GCGC 4 cut(s) 18, 121, 164, 176
Kpn2I TCCGGA 1 cut(s) 108
Ksp22I TGATCA 1 cut(s) 1207
LguI GCTCTTC 1 cut(s) 107
LmnI GCTCC 3 cut(s) 526, 599, 879
Lsp1109I GCAGC 5 cut(s) 277, 394, 883, 1153, 1330
LweI GCATC 4 cut(s) 597, 772, 1035, 1378
MabI ACCWGGT 2 cut(s) 714, 1221
MaeI CTAG 2 cut(s) 570, 1400
MaeII ACGT 2 cut(s) 76, 745
MaeIII GTNAC 4 cut(s) 934, 1285, 1349, 1424
MboII GAAGA 9 cut(s) 124, 137, 151, 217, 779, 941, 1250, 1499, 1505
MflI RGATCY 3 cut(s) 105, 1440, 1517
MhlI GDGCHC 2 cut(s) 418, 796
MluCI AATT 4 cut(s) 170, 1095, 1363, 1461
MlyI GAGTC 5 cut(s) 226, 281, 949, 1000, 1432
MmeI TCCRAC 4 cut(s) 886, 1159, 1287, 1461
MroI TCCGGA 1 cut(s) 108
MseI TTAA 1 cut(s) 1362
MslI CAYNNNNRTG 3 cut(s) 455, 759, 985
MspA1I CMGCKG 3 cut(s) 268, 1067, 1378
MspI CCGG 2 cut(s) 37, 109
MspR9I CCNGG 3 cut(s) 716, 1223, 1527
Mva1269I GAATGC 1 cut(s) 365
MvaI CCWGG 3 cut(s) 716, 1223, 1527
MvnI CGCG 4 cut(s) 20, 123, 150, 176
NcoI CCATGG 1 cut(s) 228
NlaIII CATG 8 cut(s) 100, 232, 376, 638, 669, 990, 1142, 1173
NlaIV GGNNCC 5 cut(s) 41, 439, 601, 1519, 1560
NmeAIII GCCGAG 2 cut(s) 39, 91
NmuCI GTSAC 3 cut(s) 934, 1285, 1424
NruI TCGCGA 1 cut(s) 150
NspI RCATGY 2 cut(s) 638, 1142
OliI CACNNNNGTG 1 cut(s) 455
PaeI GCATGC 1 cut(s) 638
PaeR7I CTCGAG 1 cut(s) 200
PagI TCATGA 1 cut(s) 372
PaqCI CACCTGC 1 cut(s) 839
PceI AGGCCT 1 cut(s) 156
PciSI GCTCTTC 1 cut(s) 107
PcsI WCGNNNNNNNCGW 2 cut(s) 11, 815
PctI GAATGC 1 cut(s) 365
PfeI GAWTC 2 cut(s) 563, 1572
PflFI GACNNNGTC 1 cut(s) 673
PflMI CCANNNNNTGG 1 cut(s) 1568
PkrI GCNGC 5 cut(s) 267, 409, 873, 1168, 1345
PleI GAGTC 5 cut(s) 225, 280, 949, 1000, 1431
PpsI GAGTC 5 cut(s) 225, 280, 949, 1000, 1431
PshAI GACNNNNGTC 1 cut(s) 29
Psp1406I AACGTT 1 cut(s) 745
Psp6I CCWGG 3 cut(s) 714, 1221, 1525
PspFI CCCAGC 4 cut(s) 236, 789, 1374, 1521
PspGI CCWGG 3 cut(s) 714, 1221, 1525
PspN4I GGNNCC 5 cut(s) 41, 439, 601, 1519, 1560
PspPI GGNCC 3 cut(s) 24, 31, 1115
PsuI RGATCY 3 cut(s) 105, 1440, 1517
PsyI GACNNNGTC 1 cut(s) 673
PvuII CAGCTG 3 cut(s) 268, 1067, 1378
RruI TCGCGA 1 cut(s) 150
RsaI GTAC 1 cut(s) 1220
RsaNI GTAC 1 cut(s) 1219
RseI CAYNNNNRTG 3 cut(s) 455, 759, 985
SapI GCTCTTC 1 cut(s) 107
SaqAI TTAA 1 cut(s) 1362
SatI GCNGC 5 cut(s) 266, 408, 872, 1167, 1344
Sau96I GGNCC 3 cut(s) 24, 31, 1115
SchI GAGTC 5 cut(s) 226, 281, 949, 1000, 1432
ScrFI CCNGG 3 cut(s) 716, 1223, 1527
SduI GDGCHC 2 cut(s) 418, 796
SexAI ACCWGGT 2 cut(s) 714, 1221
SfaNI GCATC 4 cut(s) 597, 772, 1035, 1378
SfcI CTRYAG 1 cut(s) 1418
Sfr274I CTCGAG 1 cut(s) 200
SinI GGWCC 3 cut(s) 24, 31, 1115
SlaI CTCGAG 1 cut(s) 200
SmiMI CAYNNNNRTG 3 cut(s) 455, 759, 985
SmlI CTYRAG 1 cut(s) 200
SmoI CTYRAG 1 cut(s) 200
SphI GCATGC 1 cut(s) 638
Sse9I AATT 4 cut(s) 170, 1095, 1363, 1461
SseBI AGGCCT 1 cut(s) 156
SsiI CCGC 7 cut(s) 34, 43, 56, 355, 389, 1346, 1534
SspI AATATT 1 cut(s) 931
SspMI CTAG 2 cut(s) 570, 1400
StuI AGGCCT 1 cut(s) 156
StyD4I CCNGG 3 cut(s) 714, 1221, 1525
StyI CCWWGG 3 cut(s) 228, 453, 1277
TaaI ACNGT 6 cut(s) 260, 675, 922, 1089, 1291, 1355
TaiI ACGT 2 cut(s) 79, 748
TaqI TCGA 3 cut(s) 201, 302, 402
TaqII GACCGA 1 cut(s) 41
TasI AATT 4 cut(s) 170, 1095, 1363, 1461
TfiI GAWTC 2 cut(s) 563, 1572
Tru1I TTAA 1 cut(s) 1362
Tru9I TTAA 1 cut(s) 1362
TscAI CASTG 6 cut(s) 601, 678, 925, 965, 1360, 1441
TseFI GTSAC 3 cut(s) 934, 1285, 1424
TseI GCWGC 5 cut(s) 265, 407, 871, 1166, 1343
Tsp45I GTSAC 3 cut(s) 934, 1285, 1424
TspDTI ATGAA 2 cut(s) 743, 780
TspGWI ACGGA 1 cut(s) 1530
TspRI CASTG 6 cut(s) 601, 678, 925, 965, 1360, 1441
Tth111I GACNNNGTC 1 cut(s) 673
Van91I CCANNNNNTGG 1 cut(s) 1568
VpaK11BI GGWCC 3 cut(s) 24, 31, 1115
XapI RAATTY 1 cut(s) 1461
XbaI TCTAGA 1 cut(s) 1399
XceI RCATGY 2 cut(s) 638, 1142
XhoI CTCGAG 1 cut(s) 200
XmiI GTMKAC 1 cut(s) 220
XspI CTAG 2 cut(s) 570, 1400
ZraI GACGTC 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.