MD16G1159100.v1.1

Belongs to the importin alpha family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008963
Physical Location & Seq
Forward (+)
12849078 .. 12852802
3725 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1159100.v1.1.491

Sequence Viewer

Length: 1458 bp
ATGGTTGCTGGGGTTTGGTCTGACGACAACAATTTGCAGCTGGAGTCCACTACGCAGTTCAGAAAACTTCTTTCGATTGAACGAAGCCCTCCGATTGAGGAAGTTATCCAATCTGGAGTGGTTCCTCGCTTTGTCGCGTTCCTCATTAGGGAGGATTTTCCACAACTTCAGTTTGAGGCTGCTTGGGCTCTTACAAACATAGCGTCAGGAACCTCAGAGAACACCAAGGTGGTGATTGAGCAGGGGGCAGTCCCAATCTTTGTAAAGCTTCTTGCTTCTCCGAGTGATGATGTTAGGGAGCAGGCTGTGTGGGCATTGGGAAATGTTGCTGGTGATTCTCCTAGATGCCGTGATCTTGTCCTCGGCAGTGGAGCCTTGATGCCGTTGCTGGCTCAGTTGAATGAGCATGCAAAGCTTTCAATGCTGAGAAATGCAACATGGACTCTGTCTAACTTTTGCAGGGGCAAGCCACAGCCTCCATTTGACCAGGTGAAGCCAGCACTTCCAGCTCTTGAACGTTTAGTTCATTCCAATGATGAAGAAGTCTTGACAGATGCCTGTTGGGCACTTTCATATCTCTCAGATGGTACAAATGACAAAATCCAAGCTGTGATTGAAGCAGGTGTCTGCGTCCGACTTGTGCAGCTCCTCTTACATCCATCTCCTTCTGTTCTCATTCCGGCACTTCGCACTGTTGGTAATATTGTGACCGGAGATGATCTTCAGACTCAGTGTATTATTAACAATGGTTCACTCCCATGCCTTCTGAGCCTGTTGACTCATAATCATAAAAAGAGTATCAAGAAAGAAGCTTGCTGGACAATCTCAAACATTACAGCTGGAAATAGGGATCAAATACAGTCTGTAATTGAAGCTGGTTTGATTGGTCCTCTGGTCAACTTGCTTCAACATGCTGAGTTTGACATAAAGAAAGAAGCTGCATGGGCAATCTCAAATGCTACTTCTGGTGGCACTGCTGATCAAATCAAGTACCTGGTTGGCGAAGGCTGTATAAAACCATTGTGTGATCTTCTTGTATGCCCTGATCCAAGGATCGTTACTGTCTGTTTAGAAGGGATGGAGAACATTCTGAAGGTTGGGGAAGCTGAAAAAGCTGCGGGTAACAGTGGAGAAGTTAACTTGTATGCCCAGCTGATTGATGATGCTGAGGGTCTAGAAAAGATTGAAAATCTACAGAGTCACGACAACACTGAGATCTATGAGAAGTCTGTAAAAATTCTTGAGACATATTGGTTGGAGGAAGACGAAGAGACATTACCAGCAGGAGATGGTACCCAGCAAGGCATCCGCTTTGAAGGGAATGAGGTTCAGGTTCCATCTGGGCTTTTAATTGAATGGAGCAGAAGTTCTGACGAGGGTCAAGTTTGGGGTCCAGTCTGGTCAAGTTGGAGTGGGTCGGGGGCATCTCGAGTGTCTGGTTTCAGTCTGGTCCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm Arm_3 IBB

Protein Analysis

486

Amino Acids

52.81

Weight (kDa)

4.7

Isoelectric Point (pI)

42.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arm PF00514 29 - 69 8.4e-10 Armadillo/beta-catenin-like repeat
HEAT_EZ PF13513 54 - 109 2.7e-10 HEAT-like repeat
Arm PF00514 71 - 110 1e-12 Armadillo/beta-catenin-like repeat
HEAT_2 PF13646 85 - 191 1.6e-06 HEAT repeats
Arm PF00514 113 - 154 5.6e-07 Armadillo/beta-catenin-like repeat
Arm PF00514 167 - 195 3.5e-07 Armadillo/beta-catenin-like repeat
Arm PF00514 198 - 236 2.9e-08 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 214 - 394 5e-09 ARMC5
Arm PF00514 240 - 280 1.3e-09 Armadillo/beta-catenin-like repeat
Arm PF00514 282 - 321 9e-12 Armadillo/beta-catenin-like repeat
Arm PF00514 326 - 363 2.1e-06 Armadillo/beta-catenin-like repeat
Arm_3 PF16186 380 - 424 6.1e-20 Atypical Arm repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0008963)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32880 AT3G06720 AT3G06720 AT4G16143 AT4G16143
fragaria_vesca FvH4_4g19270 FvH4_4g19270
malus_domestica MD13G1158900.v1.1 MD16G1159100.v1.1
prunus_persica Prupe.1G125900_v2.0.a1
pyrus_communis pycom16g13410
rosa_chinensis RchiOBHm_Chr4g0424261
rosa_laevigata RLG00000007492
rosa_multiflora Rmu_sc0006403.1_g000001
rosa_roxburghii Rroxscaffold_5G00366200
rosa_rugosa Rorug04G0194700 Rorug04G0194800
rosa_samantha Rh4AG252100 Rh4BG257500 Rh4CG268200 Rh4DG251900
rosa_wichuraiana Rw4G021740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 611
Acc36I ACCTGC 1 cut(s) 611
Acc65I GGTACC 1 cut(s) 1292
AccB1I GGYRCC 1 cut(s) 1292
AccII CGCG 1 cut(s) 137
AciI CCGC 2 cut(s) 1118, 1309
AclI AACGTT 1 cut(s) 517
AclWI GGATC 3 cut(s) 858, 1040, 1061
AcsI RAATTY 1 cut(s) 1236
AcuI CTGAAG 3 cut(s) 152, 707, 1112
AfaI GTAC 3 cut(s) 589, 992, 1294
AfiI CCNNNNNNNGG 1 cut(s) 148
AjnI CCWGG 2 cut(s) 486, 993
AleI CACNNNNGTG 1 cut(s) 227
AloI GAACNNNNNNTCC 2 cut(s) 1351, 1383
Alw26I GTCTC 2 cut(s) 1238, 1265
AlwI GGATC 3 cut(s) 858, 1040, 1061
Ama87I CYCGRG 1 cut(s) 1428
ApeKI GCWGC 5 cut(s) 37, 179, 643, 938, 1115
ApoI RAATTY 1 cut(s) 1236
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
Asp718I GGTACC 1 cut(s) 1292
AspS9I GGNCC 3 cut(s) 887, 1391, 1450
AsuHPI GGTGA 3 cut(s) 244, 344, 502
AvaI CYCGRG 1 cut(s) 1428
AvaII GGWCC 3 cut(s) 887, 1391, 1450
BaeGI GKGCMC 1 cut(s) 568
BanI GGYRCC 1 cut(s) 1292
BanII GRGCYC 1 cut(s) 190
BarI GAAGNNNNNNTAC 2 cut(s) 1260, 1292
BbsI GAAGAC 1 cut(s) 1269
BbvCI CCTCAGC 1 cut(s) 1167
BbvI GCAGC 5 cut(s) 49, 166, 655, 925, 1102
BccI CCATC 5 cut(s) 578, 667, 1072, 1283, 1345
BceAI ACGGC 2 cut(s) 333, 367
BciT130I CCWGG 2 cut(s) 488, 995
BclI TGATCA 1 cut(s) 979
BcoDI GTCTC 2 cut(s) 1238, 1265
BfaI CTAG 2 cut(s) 342, 1175
BfmI CTRYAG 1 cut(s) 1193
BfuAI ACCTGC 1 cut(s) 611
BglI GCCNNNNNGGC 1 cut(s) 563
BglII AGATCT 1 cut(s) 1215
BisI GCNGC 5 cut(s) 38, 180, 644, 939, 1116
BlsI GCNGC 5 cut(s) 39, 181, 645, 940, 1117
Bme1390I CCNGG 2 cut(s) 488, 995
Bme18I GGWCC 3 cut(s) 887, 1391, 1450
BmeT110I CYCGRG 1 cut(s) 1428
BmgT120I GGNCC 3 cut(s) 887, 1391, 1450
BmiI GGNNCC 6 cut(s) 123, 211, 373, 1294, 1335, 1392
BmrFI CCNGG 2 cut(s) 488, 995
BmsI GCATC 6 cut(s) 335, 369, 544, 1153, 1314, 1433
BoxI GACNNNNGTC 1 cut(s) 1377
BpiI GAAGAC 1 cut(s) 1269
BpmI CTGGAG 2 cut(s) 62, 135
Bpu10I CCTNAGC 1 cut(s) 1167
BpuEI CTTGAG 1 cut(s) 1262
BsaJI CCNNGG 3 cut(s) 225, 361, 1049
BsaWI WCCGGW 2 cut(s) 710, 1452
BsaXI ACNNNNNCTCC 2 cut(s) 1351, 1381
Bsc4I CCNNNNNNNGG 1 cut(s) 148
Bse1I ACTGG 1 cut(s) 1394
BseBI CCWGG 2 cut(s) 488, 995
BseDI CCNNGG 3 cut(s) 225, 361, 1049
BseGI GGATG 3 cut(s) 655, 1083, 1305
BseLI CCNNNNNNNGG 1 cut(s) 148
BseMII CTCAG 9 cut(s) 228, 407, 416, 594, 743, 758, 906, 1158, 1203
BseNI ACTGG 1 cut(s) 1394
BseRI GAGGAG 1 cut(s) 638
BseSI GKGCMC 1 cut(s) 568
BseXI GCAGC 5 cut(s) 49, 166, 655, 925, 1102
BseYI CCCAGC 3 cut(s) 8, 1149, 1296
BsgI GTGCAG 1 cut(s) 662
Bsh1236I CGCG 1 cut(s) 137
BshNI GGYRCC 1 cut(s) 1292
BsiHKCI CYCGRG 1 cut(s) 1428
BsiSI CCGG 3 cut(s) 680, 711, 1453
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 1 cut(s) 148
BsmAI GTCTC 2 cut(s) 1238, 1265
BsmFI GGGAC 1 cut(s) 236
BsoBI CYCGRG 1 cut(s) 1428
Bsp1286I GDGCHC 2 cut(s) 190, 568
Bsp143I GATC 8 cut(s) 352, 718, 850, 979, 1027, 1045, 1053, 1215
BspACI CCGC 2 cut(s) 1118, 1309
BspCNI CTCAG 9 cut(s) 227, 406, 417, 593, 742, 759, 907, 1159, 1204
BspFNI CGCG 1 cut(s) 137
BspLI GGNNCC 6 cut(s) 123, 211, 373, 1294, 1335, 1392
BspMI ACCTGC 1 cut(s) 611
BspPI GGATC 3 cut(s) 858, 1040, 1061
BspT107I GGYRCC 1 cut(s) 1292
BsrI ACTGG 1 cut(s) 1394
BssECI CCNNGG 3 cut(s) 225, 361, 1049
BssMI GATC 8 cut(s) 352, 718, 850, 979, 1027, 1045, 1053, 1215
BssT1I CCWWGG 2 cut(s) 225, 1049
Bst2UI CCWGG 2 cut(s) 488, 995
Bst4CI ACNGT 4 cut(s) 694, 861, 1063, 1127
Bst6I CTCTTC 1 cut(s) 1263
BstC8I GCNNGC 6 cut(s) 303, 390, 408, 467, 498, 814
BstDEI CTNAG 9 cut(s) 214, 393, 425, 580, 729, 767, 915, 1167, 1212
BstF5I GGATG 3 cut(s) 655, 1083, 1305
BstFNI CGCG 1 cut(s) 137
BstKTI GATC 8 cut(s) 355, 721, 853, 982, 1030, 1048, 1056, 1218
BstMAI GTCTC 2 cut(s) 1238, 1265
BstMBI GATC 8 cut(s) 352, 718, 850, 979, 1027, 1045, 1053, 1215
BstMWI GCNNNNNNNGC 9 cut(s) 185, 311, 412, 421, 506, 563, 768, 944, 1112
BstNI CCWGG 2 cut(s) 488, 995
BstNSI RCATGY 2 cut(s) 410, 914
BstPAI GACNNNNGTC 1 cut(s) 1377
BstSCI CCNGG 2 cut(s) 486, 993
BstSFI CTRYAG 1 cut(s) 1193
BstSLI GKGCMC 1 cut(s) 568
BstUI CGCG 1 cut(s) 137
BstV1I GCAGC 5 cut(s) 49, 166, 655, 925, 1102
BstV2I GAAGAC 1 cut(s) 1269
BstX2I RGATCY 1 cut(s) 1215
BstYI RGATCY 1 cut(s) 1215
BtsCI GGATG 3 cut(s) 655, 1083, 1305
BtsI GCAGTG 2 cut(s) 373, 972
BtsIMutI CAGTG 6 cut(s) 373, 690, 737, 972, 1132, 1209
BveI ACCTGC 1 cut(s) 611
Cac8I GCNNGC 6 cut(s) 303, 390, 408, 467, 498, 814
Cfr13I GGNCC 3 cut(s) 887, 1391, 1450
CseI GACGC 2 cut(s) 192, 619
CsiI ACCWGGT 2 cut(s) 486, 993
Csp6I GTAC 3 cut(s) 588, 991, 1293
CviAII CATG 5 cut(s) 407, 438, 759, 911, 942
CviQI GTAC 3 cut(s) 588, 991, 1293
DdeI CTNAG 9 cut(s) 214, 393, 425, 580, 729, 767, 915, 1167, 1212
DpnI GATC 8 cut(s) 354, 720, 852, 981, 1029, 1047, 1055, 1217
DpnII GATC 8 cut(s) 352, 718, 850, 979, 1027, 1045, 1053, 1215
Eam1104I CTCTTC 1 cut(s) 1263
EarI CTCTTC 1 cut(s) 1263
Eco130I CCWWGG 2 cut(s) 225, 1049
Eco24I GRGCYC 1 cut(s) 190
Eco47I GGWCC 3 cut(s) 887, 1391, 1450
Eco57I CTGAAG 3 cut(s) 152, 707, 1112
Eco88I CYCGRG 1 cut(s) 1428
EcoRII CCWGG 2 cut(s) 486, 993
EcoT14I CCWWGG 2 cut(s) 225, 1049
EcoT38I GRGCYC 1 cut(s) 190
ErhI CCWWGG 2 cut(s) 225, 1049
FaeI CATG 5 cut(s) 410, 441, 762, 914, 945
FaqI GGGAC 1 cut(s) 236
FatI CATG 5 cut(s) 406, 437, 758, 910, 941
FauI CCCGC 1 cut(s) 1111
FbaI TGATCA 1 cut(s) 979
Fnu4HI GCNGC 5 cut(s) 38, 180, 644, 939, 1116
FokI GGATG 3 cut(s) 642, 1090, 1292
FriOI GRGCYC 1 cut(s) 190
Fsp4HI GCNGC 5 cut(s) 38, 180, 644, 939, 1116
FspBI CTAG 2 cut(s) 342, 1175
GluI GCNGC 5 cut(s) 38, 180, 644, 939, 1116
GsaI CCCAGC 3 cut(s) 12, 1153, 1300
GsuI CTGGAG 2 cut(s) 62, 135
HapII CCGG 3 cut(s) 680, 711, 1453
HgaI GACGC 2 cut(s) 192, 619
Hin1II CATG 5 cut(s) 410, 441, 762, 914, 945
HincII GTYRAC 3 cut(s) 777, 898, 1138
HindII GTYRAC 3 cut(s) 777, 898, 1138
HindIII AAGCTT 3 cut(s) 266, 413, 810
HinfI GANTC 6 cut(s) 44, 335, 442, 727, 778, 1198
HpaI GTTAAC 1 cut(s) 1138
HpaII CCGG 3 cut(s) 680, 711, 1453
HphI GGTGA 3 cut(s) 244, 344, 502
Hpy166II GTNNAC 5 cut(s) 48, 752, 777, 898, 1138
Hpy188III TCNNGA 9 cut(s) 114, 207, 512, 547, 802, 1175, 1202, 1241, 1428
Hpy8I GTNNAC 5 cut(s) 48, 752, 777, 898, 1138
HpyAV CCTTC 6 cut(s) 675, 773, 998, 1067, 1087, 1310
HpyCH4III ACNGT 4 cut(s) 694, 861, 1063, 1127
HpyCH4IV ACGT 1 cut(s) 517
HpyCH4V TGCA 6 cut(s) 37, 410, 434, 459, 643, 941
HpyF10VI GCNNNNNNNGC 9 cut(s) 185, 311, 412, 421, 506, 563, 768, 944, 1112
HpyF3I CTNAG 9 cut(s) 214, 393, 425, 580, 729, 767, 915, 1167, 1212
HpySE526I ACGT 1 cut(s) 517
Hsp92II CATG 5 cut(s) 410, 441, 762, 914, 945
KpnI GGTACC 1 cut(s) 1296
Ksp22I TGATCA 1 cut(s) 979
KspAI GTTAAC 1 cut(s) 1138
Kzo9I GATC 8 cut(s) 352, 718, 850, 979, 1027, 1045, 1053, 1215
LmnI GCTCC 4 cut(s) 298, 371, 651, 1359
Lsp1109I GCAGC 5 cut(s) 49, 166, 655, 925, 1102
LweI GCATC 6 cut(s) 335, 369, 544, 1153, 1314, 1433
MabI ACCWGGT 2 cut(s) 486, 993
MaeI CTAG 2 cut(s) 342, 1175
MaeII ACGT 1 cut(s) 517
MaeIII GTNAC 4 cut(s) 706, 1057, 1121, 1199
MalI GATC 8 cut(s) 354, 720, 852, 981, 1029, 1047, 1055, 1217
MboI GATC 8 cut(s) 352, 718, 850, 979, 1027, 1045, 1053, 1215
MboII GAAGA 5 cut(s) 551, 713, 1022, 1274, 1280
MflI RGATCY 1 cut(s) 1215
MhlI GDGCHC 2 cut(s) 190, 568
MluCI AATT 4 cut(s) 31, 867, 1236, 1350
MlyI GAGTC 5 cut(s) 53, 436, 721, 772, 1207
MmeI TCCRAC 3 cut(s) 658, 1236, 1388
MseI TTAA 3 cut(s) 741, 1137, 1349
MslI CAYNNNNRTG 3 cut(s) 227, 531, 757
MspA1I CMGCKG 3 cut(s) 40, 839, 1153
MspI CCGG 3 cut(s) 680, 711, 1453
MspR9I CCNGG 2 cut(s) 488, 995
MvaI CCWGG 2 cut(s) 488, 995
MvnI CGCG 1 cut(s) 137
MwoI GCNNNNNNNGC 9 cut(s) 185, 311, 412, 421, 506, 563, 768, 944, 1112
NdeII GATC 8 cut(s) 352, 718, 850, 979, 1027, 1045, 1053, 1215
NlaIII CATG 5 cut(s) 410, 441, 762, 914, 945
NlaIV GGNNCC 6 cut(s) 123, 211, 373, 1294, 1335, 1392
NmeAIII GCCGAG 1 cut(s) 342
NmuCI GTSAC 2 cut(s) 706, 1199
NspI RCATGY 2 cut(s) 410, 914
OliI CACNNNNGTG 1 cut(s) 227
PaeI GCATGC 1 cut(s) 410
PaeR7I CTCGAG 1 cut(s) 1428
PaqCI CACCTGC 1 cut(s) 611
PfeI GAWTC 1 cut(s) 335
PflFI GACNNNGTC 1 cut(s) 445
PkrI GCNGC 5 cut(s) 39, 181, 645, 940, 1117
PleI GAGTC 5 cut(s) 52, 436, 721, 772, 1206
PpsI GAGTC 5 cut(s) 52, 436, 721, 772, 1206
PshAI GACNNNNGTC 1 cut(s) 1377
Psp1406I AACGTT 1 cut(s) 517
Psp6I CCWGG 2 cut(s) 486, 993
PspFI CCCAGC 3 cut(s) 8, 1149, 1296
PspGI CCWGG 2 cut(s) 486, 993
PspN4I GGNNCC 6 cut(s) 123, 211, 373, 1294, 1335, 1392
PspPI GGNCC 3 cut(s) 887, 1391, 1450
PsuI RGATCY 1 cut(s) 1215
PsyI GACNNNGTC 1 cut(s) 445
PvuII CAGCTG 3 cut(s) 40, 839, 1153
RsaI GTAC 3 cut(s) 589, 992, 1294
RsaNI GTAC 3 cut(s) 588, 991, 1293
RseI CAYNNNNRTG 3 cut(s) 227, 531, 757
SaqAI TTAA 3 cut(s) 741, 1137, 1349
SatI GCNGC 5 cut(s) 38, 180, 644, 939, 1116
Sau3AI GATC 8 cut(s) 352, 718, 850, 979, 1027, 1045, 1053, 1215
Sau96I GGNCC 3 cut(s) 887, 1391, 1450
SchI GAGTC 5 cut(s) 53, 436, 721, 772, 1207
ScrFI CCNGG 2 cut(s) 488, 995
SduI GDGCHC 2 cut(s) 190, 568
SexAI ACCWGGT 2 cut(s) 486, 993
SfaNI GCATC 6 cut(s) 335, 369, 544, 1153, 1314, 1433
SfcI CTRYAG 1 cut(s) 1193
Sfr274I CTCGAG 1 cut(s) 1428
SinI GGWCC 3 cut(s) 887, 1391, 1450
SlaI CTCGAG 1 cut(s) 1428
SmiMI CAYNNNNRTG 3 cut(s) 227, 531, 757
SmlI CTYRAG 2 cut(s) 1241, 1428
SmoI CTYRAG 2 cut(s) 1241, 1428
SphI GCATGC 1 cut(s) 410
Sse9I AATT 4 cut(s) 31, 867, 1236, 1350
SsiI CCGC 2 cut(s) 1118, 1309
SspI AATATT 1 cut(s) 703
SspMI CTAG 2 cut(s) 342, 1175
StyD4I CCNGG 2 cut(s) 486, 993
StyI CCWWGG 2 cut(s) 225, 1049
TaaI ACNGT 4 cut(s) 694, 861, 1063, 1127
TaiI ACGT 1 cut(s) 520
TaqI TCGA 2 cut(s) 74, 1429
TasI AATT 4 cut(s) 31, 867, 1236, 1350
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 3 cut(s) 741, 1137, 1349
Tru9I TTAA 3 cut(s) 741, 1137, 1349
TscAI CASTG 6 cut(s) 373, 697, 737, 979, 1132, 1216
TseFI GTSAC 2 cut(s) 706, 1199
TseI GCWGC 5 cut(s) 37, 179, 643, 938, 1115
Tsp45I GTSAC 2 cut(s) 706, 1199
TspDTI ATGAA 3 cut(s) 515, 552, 561
TspRI CASTG 6 cut(s) 373, 697, 737, 979, 1132, 1216
Tth111I GACNNNGTC 1 cut(s) 445
VpaK11BI GGWCC 3 cut(s) 887, 1391, 1450
XapI RAATTY 1 cut(s) 1236
XbaI TCTAGA 1 cut(s) 1174
XceI RCATGY 2 cut(s) 410, 914
XhoI CTCGAG 1 cut(s) 1428
XspI CTAG 2 cut(s) 342, 1175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.