Rh4DG251900

Belongs to the importin alpha family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008963
Physical Location & Seq
Reverse (-)
46669652 .. 46674692
5041 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG251900.1

Sequence Viewer

Length: 1257 bp
ATGCCTACAGCTCTTAGGTTTTGTGGTGGTGTATTTGTTACTTATTTTGTTGGTTTTTCTAATGTCCAGTTTGAGGCTGCTTGGGCTCTTACAAACATAGCGTCAGGAACATCGGACAACACCAGGGTGGTGATTGATCACGGGGCAGTCCCAATATTCGTGAAGCTTCTTGCTTCTCCGAGTGATGATGTTAGAGAGCAGGCTGTTTGGGCATTGGGAAATGTTGCCGGTGATTCTCCAAGATGTCGTGATCTAGTCCTCAGCAGTGGTGCCTTGATGCCGTTGCTGGCTCAGTTGAATGAGCATGCAAAGCTTTCAATGCTGAGAAATGCCACCTGGACACTGTCAAACTTTTGCAGGGGCAAGCCGCAGCCTCAGTTTGATCAGGTGAAGCCAGCTCTTCCAGCTCTTGAGCGTTTAGTTCATTCCAATGATGAAGAAGTCTTGACAGATGCCTGCTGGGCACTCTCATATCTCTCGGATGGTACAAATGATAAAATTCAAGCAGTCATCGAGGCAGGTGTCTGCCTGCGACTTGTGCAGCTTCTCTTACATCCATCTCCTGCGGTTCTCATTCCTGCACTTCGCACTGTTGGTAATATTGTGACAGGAGATGATCTTCAGACTCAGTGTATAATCACCAATGGTGCACTTCCATGCCTTCTGAGCCTCTTGACACATAATCATAAGAAGAGCATCAAGAAAGAAGCTTGCTGGACTATCTCAAACATTACAGCTGGAAACAGGGAACAGATACAGTACGTAATTGAAGCTGGTTTGATTGGCCCTCTTGTCAATTTGCTCCAAACTGCTGAGTTTGAGATCAAGAAAGAGGCTGCTTGGGCAATTTCGAATGCTACTTCTGGTGGCACTCATGAGCAAATTAAGATCTTGGTGACTGAAGGCTGTATAAAACCATTAAGTGATCTCCTTGTATGCCCTGATCCAAGGATAGTAACTGTCTGCTTAGAAGGGTTGGAGAACATTTTGAAGGTTGGGGAAGCTGAAAAGAGTTTGGGTAACAGTGGAACCAACCTGTATGCACAGTTGATTGATGAGGCTGATGGTCTGGAAAAGATTGAAAATCTGCAGACTCACGACAACAACGAGATCTATGAGAAGGCAGTTAAAATTCTTGAGACATATTGGTTGGAGGACGAAGACGAGACATTACCAGCAGGAGATGGTACTCAGCCAGGCTTTAACTTTGGAGGGAATGGGCCTCAAGTTCCAACTGGTGGATTCAATTTTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm Arm_3 IBB

Protein Analysis

418

Amino Acids

45.23

Weight (kDa)

4.82

Isoelectric Point (pI)

32.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_EZ PF13513 21 - 75 4.6e-09 HEAT-like repeat
Arm PF00514 38 - 76 1.6e-12 Armadillo/beta-catenin-like repeat
HEAT_2 PF13646 51 - 157 9.7e-07 HEAT repeats
Arm PF00514 79 - 120 2e-07 Armadillo/beta-catenin-like repeat
Arm PF00514 133 - 161 2.8e-07 Armadillo/beta-catenin-like repeat
Arm PF00514 164 - 202 9e-08 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 198 - 379 6.7e-09 ARMC5
Arm PF00514 206 - 246 6.8e-10 Armadillo/beta-catenin-like repeat
Arm PF00514 248 - 287 1.2e-12 Armadillo/beta-catenin-like repeat
Arm_3 PF16186 345 - 389 1.3e-21 Atypical Arm repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0008963)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32880 AT3G06720 AT3G06720 AT4G16143 AT4G16143
fragaria_vesca FvH4_4g19270 FvH4_4g19270
malus_domestica MD13G1158900.v1.1 MD16G1159100.v1.1
prunus_persica Prupe.1G125900_v2.0.a1
pyrus_communis pycom16g13410
rosa_chinensis RchiOBHm_Chr4g0424261
rosa_laevigata RLG00000007492
rosa_multiflora Rmu_sc0006403.1_g000001
rosa_roxburghii Rroxscaffold_5G00366200
rosa_rugosa Rorug04G0194700 Rorug04G0194800
rosa_samantha Rh4AG252100 Rh4BG257500 Rh4CG268200 Rh4DG251900
rosa_wichuraiana Rw4G021740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 509
Acc36I ACCTGC 1 cut(s) 509
AccB1I GGYRCC 1 cut(s) 269
AccB7I CCANNNNNTGG 1 cut(s) 1238
AciI CCGC 2 cut(s) 368, 566
AclWI GGATC 1 cut(s) 938
AcsI RAATTY 2 cut(s) 498, 1131
AcuI CTGAAG 2 cut(s) 605, 921
AfaI GTAC 3 cut(s) 487, 761, 1189
AfiI CCNNNNNNNGG 2 cut(s) 73, 1238
AgsI TTSAA 7 cut(s) 298, 318, 503, 770, 991, 1082, 1246
AjnI CCWGG 3 cut(s) 122, 335, 1195
AleI CACNNNNGTG 1 cut(s) 125
Alw21I GWGCWC 1 cut(s) 652
Alw26I GTCTC 2 cut(s) 1133, 1160
Alw44I GTGCAC 1 cut(s) 648
AlwI GGATC 1 cut(s) 938
AoxI GGCC 2 cut(s) 784, 1220
ApaLI GTGCAC 1 cut(s) 648
ApeKI GCWGC 4 cut(s) 77, 370, 541, 836
ApoI RAATTY 2 cut(s) 498, 1131
ArsI GACNNNNNNTTYG 2 cut(s) 86, 118
AspS9I GGNCC 2 cut(s) 785, 1220
AsuHPI GGTGA 5 cut(s) 142, 242, 400, 631, 907
AsuII TTCGAA 1 cut(s) 851
BaeGI GKGCMC 2 cut(s) 466, 652
BanI GGYRCC 1 cut(s) 269
BanII GRGCYC 1 cut(s) 88
BbsI GAAGAC 1 cut(s) 1167
Bbv12I GWGCWC 1 cut(s) 652
BbvCI CCTCAGC 1 cut(s) 260
BbvI GCAGC 4 cut(s) 64, 382, 553, 823
BccI CCATC 4 cut(s) 476, 565, 1058, 1178
BceAI ACGGC 1 cut(s) 265
BciT130I CCWGG 3 cut(s) 124, 337, 1197
BclI TGATCA 2 cut(s) 136, 382
BcoDI GTCTC 2 cut(s) 1133, 1160
BfaI CTAG 1 cut(s) 254
BfmI CTRYAG 2 cut(s) 6, 1088
BfuAI ACCTGC 1 cut(s) 509
BglI GCCNNNNNGGC 1 cut(s) 461
BglII AGATCT 2 cut(s) 888, 1110
BisI GCNGC 5 cut(s) 78, 368, 371, 542, 837
BlsI GCNGC 5 cut(s) 79, 369, 372, 543, 838
Bme1390I CCNGG 3 cut(s) 124, 337, 1197
BmgT120I GGNCC 2 cut(s) 785, 1220
BmiI GGNNCC 2 cut(s) 271, 1030
BmrFI CCNGG 3 cut(s) 124, 337, 1197
BmsI GCATC 3 cut(s) 267, 442, 705
BpiI GAAGAC 1 cut(s) 1167
Bpu10I CCTNAGC 1 cut(s) 260
Bpu14I TTCGAA 1 cut(s) 851
BpuEI CTTGAG 3 cut(s) 431, 1157, 1209
BsaAI YACGTR 1 cut(s) 763
BsaJI CCNNGG 2 cut(s) 123, 947
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 1238
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 2 cut(s) 67, 1240
BseBI CCWGG 3 cut(s) 124, 337, 1197
BseDI CCNNGG 2 cut(s) 123, 947
BseGI GGATG 2 cut(s) 487, 553
BseLI CCNNNNNNNGG 2 cut(s) 73, 1238
BseMII CTCAG 8 cut(s) 274, 305, 314, 389, 641, 656, 804, 1205
BseNI ACTGG 2 cut(s) 67, 1240
BseSI GKGCMC 2 cut(s) 466, 652
BseXI GCAGC 4 cut(s) 64, 382, 553, 823
BseYI CCCAGC 1 cut(s) 459
BsgI GTGCAG 2 cut(s) 560, 564
BshFI GGCC 2 cut(s) 786, 1222
BshNI GGYRCC 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 652
BsiSI CCGG 1 cut(s) 228
BslFI GGGAC 1 cut(s) 134
BslI CCNNNNNNNGG 2 cut(s) 73, 1238
BsmAI GTCTC 2 cut(s) 1133, 1160
BsmFI GGGAC 1 cut(s) 134
BsmI GAATGC 1 cut(s) 859
BsnI GGCC 2 cut(s) 786, 1222
Bsp119I TTCGAA 1 cut(s) 851
Bsp1286I GDGCHC 3 cut(s) 88, 466, 652
Bsp143I GATC 9 cut(s) 136, 250, 382, 616, 822, 888, 925, 943, 1110
BspACI CCGC 2 cut(s) 368, 566
BspANI GGCC 2 cut(s) 786, 1222
BspCNI CTCAG 8 cut(s) 273, 304, 315, 388, 640, 657, 805, 1204
BspHI TCATGA 1 cut(s) 874
BspLI GGNNCC 2 cut(s) 271, 1030
BspMAI CTGCAG 1 cut(s) 1092
BspMI ACCTGC 1 cut(s) 509
BspPI GGATC 1 cut(s) 938
BspQI GCTCTTC 2 cut(s) 405, 686
BspT104I TTCGAA 1 cut(s) 851
BspT107I GGYRCC 1 cut(s) 269
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 2 cut(s) 67, 1240
BssAI RCCGGY 1 cut(s) 227
BssECI CCNNGG 2 cut(s) 123, 947
BssMI GATC 9 cut(s) 136, 250, 382, 616, 822, 888, 925, 943, 1110
BssT1I CCWWGG 1 cut(s) 947
Bst2UI CCWGG 3 cut(s) 124, 337, 1197
Bst4CI ACNGT 6 cut(s) 345, 592, 759, 961, 1025, 1047
Bst6I CTCTTC 2 cut(s) 405, 686
BstBAI YACGTR 1 cut(s) 763
BstBI TTCGAA 1 cut(s) 851
BstC8I GCNNGC 8 cut(s) 201, 288, 306, 365, 396, 457, 530, 712
BstF5I GGATG 2 cut(s) 487, 553
BstKTI GATC 9 cut(s) 139, 253, 385, 619, 825, 891, 928, 946, 1113
BstMAI GTCTC 2 cut(s) 1133, 1160
BstMBI GATC 9 cut(s) 136, 250, 382, 616, 822, 888, 925, 943, 1110
BstMWI GCNNNNNNNGC 9 cut(s) 83, 209, 310, 319, 404, 461, 538, 666, 842
BstNI CCWGG 3 cut(s) 124, 337, 1197
BstNSI RCATGY 1 cut(s) 308
BstSCI CCNGG 3 cut(s) 122, 335, 1195
BstSFI CTRYAG 2 cut(s) 6, 1088
BstSLI GKGCMC 2 cut(s) 466, 652
BstSNI TACGTA 1 cut(s) 763
BstV1I GCAGC 4 cut(s) 64, 382, 553, 823
BstV2I GAAGAC 1 cut(s) 1167
BstX2I RGATCY 2 cut(s) 888, 1110
BstYI RGATCY 2 cut(s) 888, 1110
BsuRI GGCC 2 cut(s) 786, 1222
BtsCI GGATG 2 cut(s) 487, 553
BtsI GCAGTG 1 cut(s) 271
BtsIMutI CAGTG 5 cut(s) 271, 341, 588, 635, 1030
BveI ACCTGC 1 cut(s) 509
Cac8I GCNNGC 8 cut(s) 201, 288, 306, 365, 396, 457, 530, 712
CciI TCATGA 1 cut(s) 874
Cfr10I RCCGGY 1 cut(s) 227
Cfr13I GGNCC 2 cut(s) 785, 1220
CseI GACGC 1 cut(s) 90
Csp6I GTAC 3 cut(s) 486, 760, 1188
CviAII CATG 3 cut(s) 305, 657, 875
CviQI GTAC 3 cut(s) 486, 760, 1188
DpnI GATC 9 cut(s) 138, 252, 384, 618, 824, 890, 927, 945, 1112
DpnII GATC 9 cut(s) 136, 250, 382, 616, 822, 888, 925, 943, 1110
Eam1104I CTCTTC 2 cut(s) 405, 686
EarI CTCTTC 2 cut(s) 405, 686
Eco105I TACGTA 1 cut(s) 763
Eco130I CCWWGG 1 cut(s) 947
Eco24I GRGCYC 1 cut(s) 88
Eco57I CTGAAG 2 cut(s) 605, 921
EcoRII CCWGG 3 cut(s) 122, 335, 1195
EcoT14I CCWWGG 1 cut(s) 947
EcoT38I GRGCYC 1 cut(s) 88
ErhI CCWWGG 1 cut(s) 947
FaeI CATG 3 cut(s) 308, 660, 878
FaqI GGGAC 1 cut(s) 134
FatI CATG 3 cut(s) 304, 656, 874
FbaI TGATCA 2 cut(s) 136, 382
Fnu4HI GCNGC 5 cut(s) 78, 368, 371, 542, 837
FokI GGATG 2 cut(s) 494, 540
FriOI GRGCYC 1 cut(s) 88
Fsp4HI GCNGC 5 cut(s) 78, 368, 371, 542, 837
FspBI CTAG 1 cut(s) 254
GluI GCNGC 5 cut(s) 78, 368, 371, 542, 837
GsaI CCCAGC 1 cut(s) 463
HaeIII GGCC 2 cut(s) 786, 1222
HapII CCGG 1 cut(s) 228
HgaI GACGC 1 cut(s) 90
Hin1II CATG 3 cut(s) 308, 660, 878
HindIII AAGCTT 3 cut(s) 164, 311, 708
HinfI GANTC 4 cut(s) 233, 625, 1093, 1242
HpaII CCGG 1 cut(s) 228
HphI GGTGA 5 cut(s) 142, 242, 400, 631, 907
Hpy166II GTNNAC 1 cut(s) 650
Hpy188I TCNGA 5 cut(s) 115, 180, 481, 624, 666
Hpy8I GTNNAC 1 cut(s) 650
HpyAV CCTTC 5 cut(s) 671, 896, 965, 985, 1114
HpyCH4III ACNGT 6 cut(s) 345, 592, 759, 961, 1025, 1047
HpyCH4IV ACGT 1 cut(s) 762
HpyCH4V TGCA 7 cut(s) 308, 357, 541, 581, 650, 1043, 1090
HpyF10VI GCNNNNNNNGC 9 cut(s) 83, 209, 310, 319, 404, 461, 538, 666, 842
HpySE526I ACGT 1 cut(s) 762
Hsp92II CATG 3 cut(s) 308, 660, 878
Ksp22I TGATCA 2 cut(s) 136, 382
Kzo9I GATC 9 cut(s) 136, 250, 382, 616, 822, 888, 925, 943, 1110
LguI GCTCTTC 2 cut(s) 405, 686
LmnI GCTCC 1 cut(s) 807
Lsp1109I GCAGC 4 cut(s) 64, 382, 553, 823
LweI GCATC 3 cut(s) 267, 442, 705
MaeI CTAG 1 cut(s) 254
MaeII ACGT 1 cut(s) 762
MaeIII GTNAC 5 cut(s) 37, 604, 895, 955, 1019
MalI GATC 9 cut(s) 138, 252, 384, 618, 824, 890, 927, 945, 1112
MboI GATC 9 cut(s) 136, 250, 382, 616, 822, 888, 925, 943, 1110
MboII GAAGA 5 cut(s) 392, 449, 611, 703, 1172
MflI RGATCY 2 cut(s) 888, 1110
MhlI GDGCHC 3 cut(s) 88, 466, 652
MluCI AATT 7 cut(s) 498, 765, 796, 846, 882, 1131, 1246
MlyI GAGTC 2 cut(s) 619, 1087
MmeI TCCRAC 3 cut(s) 957, 1131, 1256
MseI TTAA 4 cut(s) 885, 920, 1128, 1203
MslI CAYNNNNRTG 3 cut(s) 125, 429, 655
MspA1I CMGCKG 1 cut(s) 737
MspI CCGG 1 cut(s) 228
MspR9I CCNGG 3 cut(s) 124, 337, 1197
Mva1269I GAATGC 1 cut(s) 859
MvaI CCWGG 3 cut(s) 124, 337, 1197
MwoI GCNNNNNNNGC 9 cut(s) 83, 209, 310, 319, 404, 461, 538, 666, 842
NdeII GATC 9 cut(s) 136, 250, 382, 616, 822, 888, 925, 943, 1110
NlaIII CATG 3 cut(s) 308, 660, 878
NlaIV GGNNCC 2 cut(s) 271, 1030
NmuCI GTSAC 2 cut(s) 604, 895
NspI RCATGY 1 cut(s) 308
NspV TTCGAA 1 cut(s) 851
OliI CACNNNNGTG 1 cut(s) 125
PaeI GCATGC 1 cut(s) 308
PagI TCATGA 1 cut(s) 874
PaqCI CACCTGC 1 cut(s) 509
PciSI GCTCTTC 2 cut(s) 405, 686
PcsI WCGNNNNNNNCGW 1 cut(s) 1104
PctI GAATGC 1 cut(s) 859
PfeI GAWTC 2 cut(s) 233, 1242
PflFI GACNNNGTC 1 cut(s) 343
PflMI CCANNNNNTGG 1 cut(s) 1238
PkrI GCNGC 5 cut(s) 79, 369, 372, 543, 838
PleI GAGTC 2 cut(s) 619, 1087
PpsI GAGTC 2 cut(s) 619, 1087
Ppu21I YACGTR 1 cut(s) 763
Psp6I CCWGG 3 cut(s) 122, 335, 1195
PspFI CCCAGC 1 cut(s) 459
PspGI CCWGG 3 cut(s) 122, 335, 1195
PspN4I GGNNCC 2 cut(s) 271, 1030
PspPI GGNCC 2 cut(s) 785, 1220
PstI CTGCAG 1 cut(s) 1092
PsuI RGATCY 2 cut(s) 888, 1110
PsyI GACNNNGTC 1 cut(s) 343
PvuII CAGCTG 1 cut(s) 737
RsaI GTAC 3 cut(s) 487, 761, 1189
RsaNI GTAC 3 cut(s) 486, 760, 1188
RseI CAYNNNNRTG 3 cut(s) 125, 429, 655
SapI GCTCTTC 2 cut(s) 405, 686
SaqAI TTAA 4 cut(s) 885, 920, 1128, 1203
SatI GCNGC 5 cut(s) 78, 368, 371, 542, 837
Sau3AI GATC 9 cut(s) 136, 250, 382, 616, 822, 888, 925, 943, 1110
Sau96I GGNCC 2 cut(s) 785, 1220
SchI GAGTC 2 cut(s) 619, 1087
ScrFI CCNGG 3 cut(s) 124, 337, 1197
SduI GDGCHC 3 cut(s) 88, 466, 652
SfaNI GCATC 3 cut(s) 267, 442, 705
SfcI CTRYAG 2 cut(s) 6, 1088
SfuI TTCGAA 1 cut(s) 851
SmiMI CAYNNNNRTG 3 cut(s) 125, 429, 655
SmlI CTYRAG 3 cut(s) 410, 1136, 1224
SmoI CTYRAG 3 cut(s) 410, 1136, 1224
SnaBI TACGTA 1 cut(s) 763
SphI GCATGC 1 cut(s) 308
Sse9I AATT 7 cut(s) 498, 765, 796, 846, 882, 1131, 1246
SsiI CCGC 2 cut(s) 368, 566
SspI AATATT 2 cut(s) 156, 601
SspMI CTAG 1 cut(s) 254
StyD4I CCNGG 3 cut(s) 122, 335, 1195
StyI CCWWGG 1 cut(s) 947
TaaI ACNGT 6 cut(s) 345, 592, 759, 961, 1025, 1047
TaiI ACGT 1 cut(s) 765
TaqI TCGA 2 cut(s) 513, 851
TasI AATT 7 cut(s) 498, 765, 796, 846, 882, 1131, 1246
TauI GCSGC 1 cut(s) 370
TfiI GAWTC 2 cut(s) 233, 1242
Tru1I TTAA 4 cut(s) 885, 920, 1128, 1203
Tru9I TTAA 4 cut(s) 885, 920, 1128, 1203
TscAI CASTG 5 cut(s) 271, 348, 595, 635, 1030
TseFI GTSAC 2 cut(s) 604, 895
TseI GCWGC 4 cut(s) 77, 370, 541, 836
Tsp45I GTSAC 2 cut(s) 604, 895
TspDTI ATGAA 2 cut(s) 413, 450
TspRI CASTG 5 cut(s) 271, 348, 595, 635, 1030
Tth111I GACNNNGTC 1 cut(s) 343
Van91I CCANNNNNTGG 1 cut(s) 1238
VneI GTGCAC 1 cut(s) 648
XapI RAATTY 2 cut(s) 498, 1131
XceI RCATGY 1 cut(s) 308
XspI CTAG 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.