Rh4CG268200

Belongs to the importin alpha family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0008963
Physical Location & Seq
Reverse (-)
52234739 .. 52238851
4113 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG268200.1

Sequence Viewer

Length: 1323 bp
ATGTCGCTCAGGCCGAACGCCAGGACTGAGGTCCGCCGGAACCGGTACAAGGTGGCGGTGGACGCCGAGGAGGGCCGTCGCCGGAGAGAGGACAACATGGTCGAGATCCGGAAGAGCAAGAGGGAGGAGAGTTTGTTAAAGAAGCGCCGCGAAGGTCTTCAAGCTCAGCAATTCCCGGCCTCTGTTCAGCCACCGAACCTCGAGAAGAAGTTGGAGAGTCTACCGTCGATGGTTGCCGGGGTTTGGTCCAGCGAAAGTCCTCTGCAGCTGGAGGCTACTACGCAGTTTCGGAAACTGCTTTCTATAGAAAGAAGCCCTCCAATTGAGGAAGTAATTCAAGCTGGGGTTGTTCCGCGGTTCGTTGAGTTCCTTGTCAGGGAGGATTATCCACAACTTCAGTTTGAGGCTGCTTGGGCTCTTACAAACATAGCATCAGGAACATCGGACAACACCAGGGTGGTGATTGATCACGGGGCAGTCCCAATATTTGTGAAGCTTCTTGCTTCTCCGAGTGATGATGTTAGAGAGCAGGCTGTTTGGGCATTGGGAAATGTTGCTGGTGATTCTCCAAGATGTCGTGATCTAGTCCTCAGCAGTGGTGCCTTGATGCCGTTGCTGGCTCAGTTGAATGAGCATGCAAAGCTTTCAATGCTGAGAAATGCCACCTGGACACTGTCAAACTTTTGCAGGGGCAAGCCGCAGCCTCAGTTTGATCAGGTGAAGCCAGCTCTTCCAGCTCTTGAGCGTTTAGTTCATTCCAATGATGAAGAAGTCTTGACAGATGCCTGCTGGGCACTCTCATATCTCTCGGATGGTACAAATGACAAAATTCAAGCAGTCATCGAGGCAGGTGTCTGCCTGCGACTTGTGCAGCTTCTCTTACATCCATCTCCTGCAGTTCTCATTCCTGCACTTCGCACTGTTGGTAATATTGTGACAGGAGATGATCTTCAGACTCAGTGTATAATCACCAATGGTGCACTTCCATGCCTTCTGAGCCTCTTGACACATAATCATAAGAAGAGCATCAAGAAAGAAGCTTGCTGGACTATCTCAAACATTACAGCTGGAAACAGGGAACAGATACAGTACGTAATTGAAGCTGGTTTGATTGGCCCTCTTGTCAATTTGCTCCAAACTGCTGAGTTTGAGATCAAGAAAGAGGCTGCTTGGGCAATTTCGAATGCTACTTCTGGTGGCACTCATGAGCAAATTAAGTGCGTAATTCACACATTATTATTATTATTATTATTTTTTACCCTACTTCCTAGTAGTAGTAGCTTATTAATTCTACTCATCCTTGTGGTGCCAGGATCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm Arm_3 IBB

Protein Analysis

440

Amino Acids

48.7

Weight (kDa)

7.08

Isoelectric Point (pI)

54.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IBB PF01749 12 - 94 1e-22 Importin beta binding domain
Arm PF00514 105 - 145 2.9e-10 Armadillo/beta-catenin-like repeat
HEAT_EZ PF13513 130 - 185 3.5e-09 HEAT-like repeat
Arm PF00514 148 - 186 1.7e-12 Armadillo/beta-catenin-like repeat
HEAT_2 PF13646 161 - 267 1.1e-06 HEAT repeats
Arm PF00514 189 - 230 2.2e-07 Armadillo/beta-catenin-like repeat
Arm PF00514 243 - 271 3e-07 Armadillo/beta-catenin-like repeat
Arm PF00514 274 - 312 9.6e-08 Armadillo/beta-catenin-like repeat
Arm PF00514 316 - 356 7.3e-10 Armadillo/beta-catenin-like repeat
Arm PF00514 358 - 397 1.3e-12 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0008963)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32880 AT3G06720 AT3G06720 AT4G16143 AT4G16143
fragaria_vesca FvH4_4g19270 FvH4_4g19270
malus_domestica MD13G1158900.v1.1 MD16G1159100.v1.1
prunus_persica Prupe.1G125900_v2.0.a1
pyrus_communis pycom16g13410
rosa_chinensis RchiOBHm_Chr4g0424261
rosa_laevigata RLG00000007492
rosa_multiflora Rmu_sc0006403.1_g000001
rosa_roxburghii Rroxscaffold_5G00366200
rosa_rugosa Rorug04G0194700 Rorug04G0194800
rosa_samantha Rh4AG252100 Rh4BG257500 Rh4CG268200 Rh4DG251900
rosa_wichuraiana Rw4G021740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 839
AasI GACNNNNNNGTC 1 cut(s) 98
Acc36I ACCTGC 1 cut(s) 839
AccB1I GGYRCC 2 cut(s) 599, 1306
AccI GTMKAC 1 cut(s) 220
AccII CGCG 2 cut(s) 150, 355
AccIII TCCGGA 1 cut(s) 108
AciI CCGC 6 cut(s) 34, 56, 148, 353, 355, 698
AclWI GGATC 2 cut(s) 100, 1321
AcsI RAATTY 1 cut(s) 828
AcuI CTGAAG 2 cut(s) 380, 935
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 3 cut(s) 47, 817, 1091
AfiI CCNNNNNNNGG 4 cut(s) 49, 88, 243, 376
AgeI ACCGGT 1 cut(s) 42
AgsI TTSAA 6 cut(s) 161, 338, 628, 648, 833, 1100
AjnI CCWGG 4 cut(s) 20, 452, 665, 1309
AleI CACNNNNGTG 1 cut(s) 455
Alw21I GWGCWC 1 cut(s) 982
Alw44I GTGCAC 1 cut(s) 978
AlwI GGATC 2 cut(s) 100, 1321
Ama87I CYCGRG 1 cut(s) 200
Aor13HI TCCGGA 1 cut(s) 108
AoxI GGCC 4 cut(s) 11, 73, 177, 1114
ApaLI GTGCAC 1 cut(s) 978
ApeKI GCWGC 5 cut(s) 265, 407, 700, 871, 1166
ApoI RAATTY 1 cut(s) 828
AseI ATTAAT 1 cut(s) 1286
AsiGI ACCGGT 1 cut(s) 42
Asp700I GAANNNNTTC 2 cut(s) 156, 333
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 4 cut(s) 31, 73, 246, 1115
AsuC2I CCSGG 2 cut(s) 176, 238
AsuHPI GGTGA 4 cut(s) 472, 572, 730, 961
AsuII TTCGAA 1 cut(s) 1181
AvaI CYCGRG 1 cut(s) 200
AvaII GGWCC 2 cut(s) 31, 246
BaeGI GKGCMC 2 cut(s) 796, 982
BanI GGYRCC 2 cut(s) 599, 1306
BanII GRGCYC 1 cut(s) 418
BbsI GAAGAC 1 cut(s) 149
Bbv12I GWGCWC 1 cut(s) 982
BbvCI CCTCAGC 1 cut(s) 590
BbvI GCAGC 5 cut(s) 277, 394, 712, 883, 1153
BccI CCATC 3 cut(s) 223, 806, 895
BceAI ACGGC 2 cut(s) 60, 595
BciT130I CCWGG 4 cut(s) 22, 454, 667, 1311
BclI TGATCA 2 cut(s) 466, 712
BcnI CCSGG 2 cut(s) 176, 238
BfaI CTAG 2 cut(s) 584, 1269
BfmI CTRYAG 3 cut(s) 263, 303, 894
BfoI RGCGCY 1 cut(s) 148
BfuAI ACCTGC 1 cut(s) 839
BglI GCCNNNNNGGC 1 cut(s) 791
BisI GCNGC 7 cut(s) 148, 266, 408, 698, 701, 872, 1167
BlpI GCTNAGC 1 cut(s) 165
BlsI GCNGC 7 cut(s) 149, 267, 409, 699, 702, 873, 1168
Bme1390I CCNGG 6 cut(s) 22, 176, 238, 454, 667, 1311
Bme18I GGWCC 2 cut(s) 31, 246
BmeT110I CYCGRG 1 cut(s) 200
BmgT120I GGNCC 4 cut(s) 31, 73, 246, 1115
BmiI GGNNCC 3 cut(s) 41, 601, 1308
BmrFI CCNGG 6 cut(s) 22, 176, 238, 454, 667, 1311
BmsI GCATC 4 cut(s) 440, 597, 772, 1035
BoxI GACNNNNGTC 1 cut(s) 29
BpiI GAAGAC 1 cut(s) 149
BpmI CTGGAG 1 cut(s) 290
Bpu10I CCTNAGC 2 cut(s) 8, 590
Bpu1102I GCTNAGC 1 cut(s) 165
Bpu14I TTCGAA 1 cut(s) 1181
BpuEI CTTGAG 1 cut(s) 761
BpuMI CCSGG 2 cut(s) 176, 238
BsaAI YACGTR 1 cut(s) 1093
BsaHI GRCGYC 1 cut(s) 63
BsaJI CCNNGG 4 cut(s) 66, 237, 353, 453
BsaWI WCCGGW 2 cut(s) 42, 108
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 88, 243, 376
Bse118I RCCGGY 1 cut(s) 42
BseAI TCCGGA 1 cut(s) 108
BseBI CCWGG 4 cut(s) 22, 454, 667, 1311
BseDI CCNNGG 4 cut(s) 66, 237, 353, 453
BseGI GGATG 3 cut(s) 817, 883, 1296
BseLI CCNNNNNNNGG 4 cut(s) 49, 88, 243, 376
BseRI GAGGAG 2 cut(s) 83, 140
BseSI GKGCMC 2 cut(s) 796, 982
BseXI GCAGC 5 cut(s) 277, 394, 712, 883, 1153
BseYI CCCAGC 2 cut(s) 341, 789
BsgI GTGCAG 2 cut(s) 890, 894
Bsh1236I CGCG 2 cut(s) 150, 355
BshFI GGCC 4 cut(s) 13, 75, 179, 1116
BshNI GGYRCC 2 cut(s) 599, 1306
BshTI ACCGGT 1 cut(s) 42
BsiHKAI GWGCWC 1 cut(s) 982
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 6 cut(s) 37, 43, 82, 109, 176, 237
BslFI GGGAC 1 cut(s) 464
BslI CCNNNNNNNGG 4 cut(s) 49, 88, 243, 376
BsmFI GGGAC 1 cut(s) 464
BsmI GAATGC 1 cut(s) 1189
BsnI GGCC 4 cut(s) 13, 75, 179, 1116
BsoBI CYCGRG 1 cut(s) 200
Bsp119I TTCGAA 1 cut(s) 1181
Bsp1286I GDGCHC 3 cut(s) 418, 796, 982
Bsp13I TCCGGA 1 cut(s) 108
Bsp143I GATC 7 cut(s) 105, 466, 580, 712, 946, 1152, 1313
Bsp1720I GCTNAGC 1 cut(s) 165
BspACI CCGC 6 cut(s) 34, 56, 148, 353, 355, 698
BspANI GGCC 4 cut(s) 13, 75, 179, 1116
BspEI TCCGGA 1 cut(s) 108
BspFNI CGCG 2 cut(s) 150, 355
BspHI TCATGA 1 cut(s) 1204
BspLI GGNNCC 3 cut(s) 41, 601, 1308
BspMAI CTGCAG 2 cut(s) 267, 898
BspMI ACCTGC 1 cut(s) 839
BspPI GGATC 2 cut(s) 100, 1321
BspQI GCTCTTC 3 cut(s) 107, 735, 1016
BspT104I TTCGAA 1 cut(s) 1181
BspT107I GGYRCC 2 cut(s) 599, 1306
BsrFI RCCGGY 1 cut(s) 42
BssAI RCCGGY 1 cut(s) 42
BssECI CCNNGG 4 cut(s) 66, 237, 353, 453
BssMI GATC 7 cut(s) 105, 466, 580, 712, 946, 1152, 1313
BssNI GRCGYC 1 cut(s) 63
Bst2UI CCWGG 4 cut(s) 22, 454, 667, 1311
Bst4CI ACNGT 4 cut(s) 225, 675, 922, 1089
Bst6I CTCTTC 3 cut(s) 107, 735, 1016
BstACI GRCGYC 1 cut(s) 63
BstBAI YACGTR 1 cut(s) 1093
BstBI TTCGAA 1 cut(s) 1181
BstC8I GCNNGC 8 cut(s) 531, 618, 636, 695, 726, 787, 860, 1042
BstDSI CCRYGG 1 cut(s) 353
BstF5I GGATG 3 cut(s) 817, 883, 1296
BstFNI CGCG 2 cut(s) 150, 355
BstH2I RGCGCY 1 cut(s) 148
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 7 cut(s) 108, 469, 583, 715, 949, 1155, 1316
BstMBI GATC 7 cut(s) 105, 466, 580, 712, 946, 1152, 1313
BstNI CCWGG 4 cut(s) 22, 454, 667, 1311
BstNSI RCATGY 1 cut(s) 638
BstPAI GACNNNNGTC 1 cut(s) 29
BstSCI CCNGG 6 cut(s) 20, 174, 236, 452, 665, 1309
BstSFI CTRYAG 3 cut(s) 263, 303, 894
BstSLI GKGCMC 2 cut(s) 796, 982
BstSNI TACGTA 1 cut(s) 1093
BstUI CGCG 2 cut(s) 150, 355
BstV1I GCAGC 5 cut(s) 277, 394, 712, 883, 1153
BstV2I GAAGAC 1 cut(s) 149
BstX2I RGATCY 2 cut(s) 105, 1313
BstXI CCANNNNNNTGG 1 cut(s) 1317
BstYI RGATCY 2 cut(s) 105, 1313
BsuRI GGCC 4 cut(s) 13, 75, 179, 1116
BtgI CCRYGG 1 cut(s) 353
BtsCI GGATG 3 cut(s) 817, 883, 1296
BtsI GCAGTG 1 cut(s) 601
BtsIMutI CAGTG 4 cut(s) 601, 671, 918, 965
BveI ACCTGC 1 cut(s) 839
Cac8I GCNNGC 8 cut(s) 531, 618, 636, 695, 726, 787, 860, 1042
CciI TCATGA 1 cut(s) 1204
CfoI GCGC 1 cut(s) 147
Cfr10I RCCGGY 1 cut(s) 42
Cfr13I GGNCC 4 cut(s) 31, 73, 246, 1115
Cfr42I CCGCGG 1 cut(s) 356
CseI GACGC 1 cut(s) 71
Csp6I GTAC 3 cut(s) 46, 816, 1090
CspAI ACCGGT 1 cut(s) 42
CviAII CATG 4 cut(s) 97, 635, 987, 1205
CviQI GTAC 3 cut(s) 46, 816, 1090
DpnI GATC 7 cut(s) 107, 468, 582, 714, 948, 1154, 1315
DpnII GATC 7 cut(s) 105, 466, 580, 712, 946, 1152, 1313
DrdI GACNNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 98
Eam1104I CTCTTC 3 cut(s) 107, 735, 1016
EarI CTCTTC 3 cut(s) 107, 735, 1016
EciI GGCGGA 1 cut(s) 23
Eco105I TACGTA 1 cut(s) 1093
Eco24I GRGCYC 1 cut(s) 418
Eco47I GGWCC 2 cut(s) 31, 246
Eco57I CTGAAG 2 cut(s) 380, 935
Eco88I CYCGRG 1 cut(s) 200
EcoRII CCWGG 4 cut(s) 20, 452, 665, 1309
EcoT38I GRGCYC 1 cut(s) 418
FaeI CATG 4 cut(s) 100, 638, 990, 1208
FaqI GGGAC 1 cut(s) 464
FatI CATG 4 cut(s) 96, 634, 986, 1204
FbaI TGATCA 2 cut(s) 466, 712
FblI GTMKAC 1 cut(s) 220
Fnu4HI GCNGC 7 cut(s) 148, 266, 408, 698, 701, 872, 1167
FokI GGATG 3 cut(s) 824, 870, 1283
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 7 cut(s) 148, 266, 408, 698, 701, 872, 1167
FspBI CTAG 2 cut(s) 584, 1269
GlaI GCGC 1 cut(s) 146
GluI GCNGC 7 cut(s) 148, 266, 408, 698, 701, 872, 1167
GsaI CCCAGC 2 cut(s) 345, 793
GsuI CTGGAG 1 cut(s) 290
HaeII RGCGCY 1 cut(s) 148
HaeIII GGCC 4 cut(s) 13, 75, 179, 1116
HapII CCGG 6 cut(s) 37, 43, 82, 109, 176, 237
HgaI GACGC 1 cut(s) 71
HhaI GCGC 1 cut(s) 147
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 4 cut(s) 100, 638, 990, 1208
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HindIII AAGCTT 3 cut(s) 494, 641, 1038
HinfI GANTC 3 cut(s) 217, 563, 955
HpaII CCGG 6 cut(s) 37, 43, 82, 109, 176, 237
HphI GGTGA 4 cut(s) 472, 572, 730, 961
Hpy166II GTNNAC 3 cut(s) 61, 221, 980
Hpy188I TCNGA 6 cut(s) 291, 445, 510, 811, 954, 996
Hpy8I GTNNAC 3 cut(s) 61, 221, 980
Hpy99I CGWCG 2 cut(s) 81, 229
HpyAV CCTTC 2 cut(s) 146, 1001
HpyCH4III ACNGT 4 cut(s) 225, 675, 922, 1089
HpyCH4IV ACGT 1 cut(s) 1092
HpyCH4V TGCA 7 cut(s) 265, 638, 687, 871, 896, 911, 980
HpySE526I ACGT 1 cut(s) 1092
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 4 cut(s) 100, 638, 990, 1208
HspAI GCGC 1 cut(s) 145
Kpn2I TCCGGA 1 cut(s) 108
Ksp22I TGATCA 2 cut(s) 466, 712
KspI CCGCGG 1 cut(s) 356
Kzo9I GATC 7 cut(s) 105, 466, 580, 712, 946, 1152, 1313
LguI GCTCTTC 3 cut(s) 107, 735, 1016
LmnI GCTCC 1 cut(s) 1137
Lsp1109I GCAGC 5 cut(s) 277, 394, 712, 883, 1153
LweI GCATC 4 cut(s) 440, 597, 772, 1035
MaeI CTAG 2 cut(s) 584, 1269
MaeII ACGT 1 cut(s) 1092
MaeIII GTNAC 1 cut(s) 934
MalI GATC 7 cut(s) 107, 468, 582, 714, 948, 1154, 1315
MboI GATC 7 cut(s) 105, 466, 580, 712, 946, 1152, 1313
MboII GAAGA 7 cut(s) 124, 149, 217, 722, 779, 941, 1033
MfeI CAATTG 1 cut(s) 321
MflI RGATCY 2 cut(s) 105, 1313
MhlI GDGCHC 3 cut(s) 418, 796, 982
MlyI GAGTC 2 cut(s) 226, 949
MmeI TCCRAC 1 cut(s) 192
MroI TCCGGA 1 cut(s) 108
MroXI GAANNNNTTC 2 cut(s) 156, 333
MseI TTAA 3 cut(s) 137, 1215, 1286
MslI CAYNNNNRTG 4 cut(s) 455, 759, 985, 1301
MspA1I CMGCKG 3 cut(s) 268, 355, 1067
MspI CCGG 6 cut(s) 37, 43, 82, 109, 176, 237
MspR9I CCNGG 6 cut(s) 22, 176, 238, 454, 667, 1311
MunI CAATTG 1 cut(s) 321
Mva1269I GAATGC 1 cut(s) 1189
MvaI CCWGG 4 cut(s) 22, 454, 667, 1311
MvnI CGCG 2 cut(s) 150, 355
NciI CCSGG 2 cut(s) 176, 238
NdeII GATC 7 cut(s) 105, 466, 580, 712, 946, 1152, 1313
NlaIII CATG 4 cut(s) 100, 638, 990, 1208
NlaIV GGNNCC 3 cut(s) 41, 601, 1308
NmeAIII GCCGAG 1 cut(s) 91
NmuCI GTSAC 1 cut(s) 934
NspI RCATGY 1 cut(s) 638
NspV TTCGAA 1 cut(s) 1181
OliI CACNNNNGTG 1 cut(s) 455
PaeI GCATGC 1 cut(s) 638
PaeR7I CTCGAG 1 cut(s) 200
PagI TCATGA 1 cut(s) 1204
PaqCI CACCTGC 1 cut(s) 839
PciSI GCTCTTC 3 cut(s) 107, 735, 1016
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PctI GAATGC 1 cut(s) 1189
PdmI GAANNNNTTC 2 cut(s) 156, 333
PfeI GAWTC 1 cut(s) 563
PflFI GACNNNGTC 1 cut(s) 673
PinAI ACCGGT 1 cut(s) 42
PkrI GCNGC 7 cut(s) 149, 267, 409, 699, 702, 873, 1168
PleI GAGTC 2 cut(s) 225, 949
PpsI GAGTC 2 cut(s) 225, 949
Ppu21I YACGTR 1 cut(s) 1093
PshAI GACNNNNGTC 1 cut(s) 29
PshBI ATTAAT 1 cut(s) 1286
Psp6I CCWGG 4 cut(s) 20, 452, 665, 1309
PspFI CCCAGC 2 cut(s) 341, 789
PspGI CCWGG 4 cut(s) 20, 452, 665, 1309
PspN4I GGNNCC 3 cut(s) 41, 601, 1308
PspPI GGNCC 4 cut(s) 31, 73, 246, 1115
PstI CTGCAG 2 cut(s) 267, 898
PsuI RGATCY 2 cut(s) 105, 1313
PsyI GACNNNGTC 1 cut(s) 673
PvuII CAGCTG 2 cut(s) 268, 1067
RsaI GTAC 3 cut(s) 47, 817, 1091
RsaNI GTAC 3 cut(s) 46, 816, 1090
RseI CAYNNNNRTG 4 cut(s) 455, 759, 985, 1301
SacII CCGCGG 1 cut(s) 356
SapI GCTCTTC 3 cut(s) 107, 735, 1016
SaqAI TTAA 3 cut(s) 137, 1215, 1286
SatI GCNGC 7 cut(s) 148, 266, 408, 698, 701, 872, 1167
Sau3AI GATC 7 cut(s) 105, 466, 580, 712, 946, 1152, 1313
Sau96I GGNCC 4 cut(s) 31, 73, 246, 1115
SchI GAGTC 2 cut(s) 226, 949
ScrFI CCNGG 6 cut(s) 22, 176, 238, 454, 667, 1311
SduI GDGCHC 3 cut(s) 418, 796, 982
SfaNI GCATC 4 cut(s) 440, 597, 772, 1035
SfcI CTRYAG 3 cut(s) 263, 303, 894
Sfr274I CTCGAG 1 cut(s) 200
Sfr303I CCGCGG 1 cut(s) 356
SfuI TTCGAA 1 cut(s) 1181
SgrBI CCGCGG 1 cut(s) 356
SinI GGWCC 2 cut(s) 31, 246
SlaI CTCGAG 1 cut(s) 200
SmiMI CAYNNNNRTG 4 cut(s) 455, 759, 985, 1301
SmlI CTYRAG 2 cut(s) 200, 740
SmoI CTYRAG 2 cut(s) 200, 740
SnaBI TACGTA 1 cut(s) 1093
SphI GCATGC 1 cut(s) 638
SsiI CCGC 6 cut(s) 34, 56, 148, 353, 355, 698
SspI AATATT 2 cut(s) 486, 931
SspMI CTAG 2 cut(s) 584, 1269
StyD4I CCNGG 6 cut(s) 20, 174, 236, 452, 665, 1309
TaaI ACNGT 4 cut(s) 225, 675, 922, 1089
TaiI ACGT 1 cut(s) 1095
TaqI TCGA 5 cut(s) 102, 201, 227, 843, 1181
TauI GCSGC 2 cut(s) 150, 700
TfiI GAWTC 1 cut(s) 563
Tru1I TTAA 3 cut(s) 137, 1215, 1286
Tru9I TTAA 3 cut(s) 137, 1215, 1286
TscAI CASTG 4 cut(s) 601, 678, 925, 965
TseFI GTSAC 1 cut(s) 934
TseI GCWGC 5 cut(s) 265, 407, 700, 871, 1166
Tsp45I GTSAC 1 cut(s) 934
TspDTI ATGAA 2 cut(s) 743, 780
TspRI CASTG 4 cut(s) 601, 678, 925, 965
Tth111I GACNNNGTC 1 cut(s) 673
VneI GTGCAC 1 cut(s) 978
VpaK11BI GGWCC 2 cut(s) 31, 246
VspI ATTAAT 1 cut(s) 1286
XapI RAATTY 1 cut(s) 828
XceI RCATGY 1 cut(s) 638
XhoI CTCGAG 1 cut(s) 200
XmiI GTMKAC 1 cut(s) 220
XmnI GAANNNNTTC 2 cut(s) 156, 333
XspI CTAG 2 cut(s) 584, 1269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.