Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003389

Physical Location & Seq

Genomic Coordinates Forward (+)

15046982 .. 15053136

Gene Structure 6155 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1180700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGGAACAGCTAGTCAACTTTATTATTCGGCCTCCCAGGGCTGAGTACGACCCACAAAATGATTTGTTGGATCAAGAGTTCATACTGAAAGGGAAATGGTACCAAAGAAAAGATGTGGAGTTGAAAAATAGTCGGGGAGATGTCCTGAAGTGTAGCCATTACACGCCCATTGTTAGTCCAGAAGAAAAGCCTTTGCCTTGTGTAATATACTGTCATGGAAACAGTGGGTGCAGGGCTGATGCCAGTGAAGCAGCCATTATTTTGCTTCCATCAAATATTACAGTTTTCACTCTTGATTTCTCCGGGTCCGGAATCTCCGGAGGAGATCATGTGACTTTAGGGTGGAATGAAGACGACCTGAAGACTGTGGTTGATTATTTGCGAGCAGATGGAAATGTATCTTTGATTGGTTTGTGGGGCCGTTCAATGGGTGCTGTTACTAGTCTTTTGTATGGAGCTGAGGATCCTTCAATAGCAGGAATGGTTCTTGACAGTCCATTCTCTGATTTGGTTGATTTGATGATGGAATTGGTGGACACTTACAAAATTCGTTTGCCTAAGTTCACTGTGAAGTTTGCAATCCAATACATGCGGAGAGCTATCCAGAAAAAGGCAAAATTTGACATAATGAACCTGAACACCATTAAGGTGGCAAGCTCTTGCTTTGTTCCAGCTTTGTTTGGGCATGCCATTGATGATGATTTCATACAACCCCATCATTCAGATCGTATATTTGAGGCGTATATGGGAGACAAAAACATCATCAAATTTGAGGGAGATCACAATTCTCCACGACCTCAATTCTACTTTGATTCTATAAACATCTTTTTCCACAATGTTTTGCAACCCCCGGAGGATGAGGTTGTGGAAACATTTTTTGACACTTTACACGAGACCTTTGGTAAGGGTAGTTGGAGAAGTGTGCACACAGCTGATTATGATCACGGATGTTCAACTGCTGCTACAGAAGCACCACCTAGCAGCGGCCAACAGGATGCCATTAGACAACTCCGTTCAAGAAGACCCATGAGTCGGATGGAGGTTCCTTCTGGCATTTCATCTACCGATGGCCAATCCGAATCCAAGGATGGACAAACTGATAATGGCCCATCATCTTCTGATATGATTAGCTTTGAATTTGCTAATGGCAGTCCTTGTGGGCCCCATGTTCCAACCACGATGGACGATGATCAGTATGTAGAATACCAGCTTGATGACCTGGAAGGTTTTCCAAGTGATGTCGATGAGGAAGAAAGGATGTTAATGGAAGCAGTGATGTTATCACTAAAAGACTTAGAAATGAGAGGCCCTCAGGCAGATGAACCACCGCCCTGGGATACCCCTGATTCTTTAAATTTGTCGCAAAAAGATGACCAGCCTGATGATGCATCTACTGCAGAGAAGCGAGAGCTGTTGGAGACAGAATCTACTTCCACTTCAGTTGGGCACTTGGGATCCTCGAAAACAGAATCTATTTCTACTTCAGTAAGTAAATCCCACGACTCGAGACCTGAAGATCAAACTCCTACAAGGGTTCCAACAACAGAACCTGCATCACAAACGCCTCTATCCATAATGGATGGGAGCGCTGGGGCATCTAGCCAAAGCGATACATCAGAAAGCATCCAAAGAACATCAGATAGTGACTTGTCAGCCAAGACGACAGCCACAGTAACGGTGGTAAAAAATCCTGCAAGCAATGTCATGGATGGTTTGATACGGCGTTGGGATTTTAACTTCTTTAGAAACAACCACAGTCGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hydrolase_4

Protein Analysis

588

Amino Acids

65.11

Weight (kDa)

4.64

Isoelectric Point (pI)

50.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase_4	PF12146	64 - 174	3.2e-10	Serine aminopeptidase, S33
Abhydrolase_1	PF00561	66 - 181	9e-09	alpha/beta hydrolase fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003389)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23540 AT3G23540 AT3G23540 AT4G14290 AT4G14290 AT4G14290 AT4G14290 AT4G14290
fragaria_vesca	FvH4_4g14200 FvH4_4g14200 FvH4_4g14200 FvH4_4g14200 FvH4_4g14200 FvH4_4g14200 FvH4_4g14200
malus_domestica	MD13G1180700.v1.1 MD16G1181400.v1.1
prunus_persica	Prupe.1G148800_v2.0.a1 Prupe.1G148800_v2.0.a1
pyrus_communis	pycom12g09460 pycom12g09470 pycom12g09480 pycom13g15590 pycom16g15220
rosa_chinensis	RchiOBHm_Chr4g0419001
rosa_laevigata	RLG00000007834
rosa_multiflora	Rmu_sc0010159.1_g000002
rosa_roxburghii	Rroxscaffold_5G00362060
rosa_rugosa	Rorug04G0156000 Rorug04G0156000 Rorug04G0156000 Rorug04G0156100
rosa_samantha	Rh4AG217800 Rh4BG212400 Rh4DG216400
rosa_wichuraiana	Rw4G018620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1029
Acc36I	ACCTGC	1 cut(s)	1558
Acc65I	GGTACC	1 cut(s)	99
AccB1I	GGYRCC	1 cut(s)	99
AccIII	TCCGGA	2 cut(s)	308, 317
AciI	CCGC	3 cut(s)	592, 984, 1328
AclWI	GGATC	5 cut(s)	78, 458, 471, 1449, 1462
AcoI	YGGCCR	2 cut(s)	985, 1069
AcsI	RAATTY	5 cut(s)	546, 617, 767, 1136, 1354
AcuI	CTGAAG	5 cut(s)	167, 380, 1422, 1467, 1533
AfaI	GTAC	2 cut(s)	47, 101
AfeI	AGCGCT	1 cut(s)	1588
AfiI	CCNNNNNNNGG	4 cut(s)	427, 610, 983, 1032
AgsI	TTSAA	6 cut(s)	124, 426, 471, 954, 1017, 1136
AhlI	ACTAGT	1 cut(s)	440
AjnI	CCWGG	3 cut(s)	35, 1218, 1331
AloI	GAACNNNNNNTCC	2 cut(s)	62, 94
AluBI	AGCT	9 cut(s)	10, 458, 599, 657, 674, 932, 1131, 1210, 1411
AluI	AGCT	9 cut(s)	10, 458, 599, 657, 674, 932, 1131, 1210, 1411
Alw21I	GWGCWC	1 cut(s)	927
Alw26I	GTCTC	4 cut(s)	744, 887, 1412, 1501
Alw44I	GTGCAC	1 cut(s)	923
AlwI	GGATC	5 cut(s)	78, 458, 471, 1449, 1462
AlwNI	CAGNNNCTG	1 cut(s)	1550
Ama87I	CYCGRG	1 cut(s)	1504
Aor13HI	TCCGGA	2 cut(s)	308, 317
Aor51HI	AGCGCT	1 cut(s)	1588
AoxI	GGCC	7 cut(s)	29, 418, 985, 1069, 1105, 1160, 1306
ApaI	GGGCCC	1 cut(s)	1164
ApaLI	GTGCAC	1 cut(s)	923
ApeKI	GCWGC	3 cut(s)	251, 959, 981
ApoI	RAATTY	5 cut(s)	546, 617, 767, 1136, 1354
Asp700I	GAANNNNTTC	1 cut(s)	1227
Asp718I	GGTACC	1 cut(s)	99
AspLEI	GCGC	1 cut(s)	1589
AspS9I	GGNCC	6 cut(s)	306, 418, 1106, 1160, 1161, 1307
AsuC2I	CCSGG	2 cut(s)	304, 851
AvaI	CYCGRG	1 cut(s)	1504
AvaII	GGWCC	1 cut(s)	306
AxyI	CCTNAGG	1 cut(s)	1311
BaeGI	GKGCMC	3 cut(s)	927, 1164, 1449
BalI	TGGCCA	1 cut(s)	1071
BamHI	GGATCC	2 cut(s)	463, 1454
BanI	GGYRCC	1 cut(s)	99
BanII	GRGCYC	1 cut(s)	1164
BauI	CACGAG	1 cut(s)	890
BbsI	GAAGAC	3 cut(s)	357, 368, 1027
Bbv12I	GWGCWC	1 cut(s)	927
BbvCI	CCTCAGC	1 cut(s)	459
BbvI	GCAGC	3 cut(s)	263, 946, 993
BceAI	ACGGC	2 cut(s)	405, 1736
BciT130I	CCWGG	3 cut(s)	37, 1220, 1333
BciVI	GTATCC	1 cut(s)	1330
BclI	TGATCA	2 cut(s)	940, 1189
BcnI	CCSGG	2 cut(s)	304, 851
BcoDI	GTCTC	4 cut(s)	744, 887, 1412, 1501
BcuI	ACTAGT	1 cut(s)	440
BfaI	CTAG	4 cut(s)	11, 441, 978, 1599
BfmI	CTRYAG	2 cut(s)	963, 1395
BfoI	RGCGCY	1 cut(s)	1590
BfuAI	ACCTGC	1 cut(s)	1558
BfuI	GTATCC	1 cut(s)	1330
BisI	GCNGC	4 cut(s)	252, 960, 982, 985
BlsI	GCNGC	4 cut(s)	253, 961, 983, 986
Bme1390I	CCNGG	5 cut(s)	37, 304, 851, 1220, 1333
Bme18I	GGWCC	1 cut(s)	306
BmeT110I	CYCGRG	1 cut(s)	1504
BmgT120I	GGNCC	6 cut(s)	306, 418, 1106, 1160, 1161, 1307
BmiI	GGNNCC	9 cut(s)	101, 307, 419, 465, 1044, 1162, 1163, 1456, 1536
BmrFI	CCNGG	5 cut(s)	37, 304, 851, 1220, 1333
BmsI	GCATC	7 cut(s)	229, 985, 1375, 1397, 1562, 1604, 1632
BpiI	GAAGAC	3 cut(s)	357, 368, 1027
BplI	GAGNNNNNCTC	2 cut(s)	1294, 1326
Bpu10I	CCTNAGC	1 cut(s)	459
BpuMI	CCSGG	2 cut(s)	304, 851
BsaBI	GATNNNNATC	1 cut(s)	939
BsaI	GGTCTC	2 cut(s)	887, 1501
BsaJI	CCNNGG	6 cut(s)	35, 36, 849, 1083, 1331, 1332
BsaWI	WCCGGW	2 cut(s)	308, 317
BsaXI	ACNNNNNCTCC	2 cut(s)	315, 345
Bsc4I	CCNNNNNNNGG	4 cut(s)	427, 610, 983, 1032
Bse1I	ACTGG	1 cut(s)	243
Bse21I	CCTNAGG	1 cut(s)	1311
Bse3DI	GCAATG	1 cut(s)	1705
Bse8I	GATNNNNATC	1 cut(s)	939
BseAI	TCCGGA	2 cut(s)	308, 317
BseBI	CCWGG	3 cut(s)	37, 1220, 1333
BseDI	CCNNGG	6 cut(s)	35, 36, 849, 1083, 1331, 1332
BseGI	GGATG	9 cut(s)	862, 953, 1000, 1041, 1093, 1263, 1585, 1623, 1714
BseJI	GATNNNNATC	1 cut(s)	939
BseLI	CCNNNNNNNGG	4 cut(s)	427, 610, 983, 1032
BseMI	GCAATG	1 cut(s)	1705
BseMII	CTCAG	3 cut(s)	33, 450, 1325
BseNI	ACTGG	1 cut(s)	243
BseRI	GAGGAG	1 cut(s)	336
BseSI	GKGCMC	3 cut(s)	927, 1164, 1449
BseXI	GCAGC	3 cut(s)	263, 946, 993
BseYI	CCCAGC	1 cut(s)	1589
BsgI	GTGCAG	1 cut(s)	250
BshFI	GGCC	7 cut(s)	31, 420, 987, 1071, 1107, 1162, 1308
BshNI	GGYRCC	1 cut(s)	99
BsiHKAI	GWGCWC	1 cut(s)	927
BsiHKCI	CYCGRG	1 cut(s)	1504
BsiSI	CCGG	4 cut(s)	303, 309, 318, 851
BslI	CCNNNNNNNGG	4 cut(s)	427, 610, 983, 1032
BsmAI	GTCTC	4 cut(s)	744, 887, 1412, 1501
BsnI	GGCC	7 cut(s)	31, 420, 987, 1071, 1107, 1162, 1308
Bso31I	GGTCTC	2 cut(s)	887, 1501
BsoBI	CYCGRG	1 cut(s)	1504
Bsp120I	GGGCCC	1 cut(s)	1160
Bsp1286I	GDGCHC	3 cut(s)	927, 1164, 1449
Bsp13I	TCCGGA	2 cut(s)	308, 317
Bsp143I	GATC	9 cut(s)	70, 325, 463, 724, 778, 940, 1189, 1454, 1516
BspACI	CCGC	3 cut(s)	592, 984, 1328
BspANI	GGCC	7 cut(s)	31, 420, 987, 1071, 1107, 1162, 1308
BspCNI	CTCAG	3 cut(s)	34, 451, 1324
BspEI	TCCGGA	2 cut(s)	308, 317
BspLI	GGNNCC	9 cut(s)	101, 307, 419, 465, 1044, 1162, 1163, 1456, 1536
BspMAI	CTGCAG	1 cut(s)	1399
BspMI	ACCTGC	1 cut(s)	1558
BspPI	GGATC	5 cut(s)	78, 458, 471, 1449, 1462
BspT107I	GGYRCC	1 cut(s)	99
BspTNI	GGTCTC	2 cut(s)	887, 1501
BsrDI	GCAATG	1 cut(s)	1705
BsrI	ACTGG	1 cut(s)	243
BssECI	CCNNGG	6 cut(s)	35, 36, 849, 1083, 1331, 1332
BssMI	GATC	9 cut(s)	70, 325, 463, 724, 778, 940, 1189, 1454, 1516
BssSI	CACGAG	1 cut(s)	890
BssT1I	CCWWGG	1 cut(s)	1083
Bst2BI	CACGAG	1 cut(s)	890
Bst2UI	CCWGG	3 cut(s)	37, 1220, 1333
Bst4CI	ACNGT	9 cut(s)	212, 224, 283, 367, 494, 568, 1672, 1678, 1757
BstAPI	GCANNNNNTGC	1 cut(s)	1394
BstC8I	GCNNGC	4 cut(s)	384, 655, 687, 1696
BstDEI	CTNAG	5 cut(s)	42, 459, 558, 1294, 1311
BstF5I	GGATG	9 cut(s)	862, 953, 1000, 1041, 1093, 1263, 1585, 1623, 1714
BstH2I	RGCGCY	1 cut(s)	1590
BstHHI	GCGC	1 cut(s)	1589
BstKTI	GATC	9 cut(s)	73, 328, 466, 727, 781, 943, 1192, 1457, 1519
BstMAI	GTCTC	4 cut(s)	744, 887, 1412, 1501
BstMBI	GATC	9 cut(s)	70, 325, 463, 724, 778, 940, 1189, 1454, 1516
BstMWI	GCNNNNNNNGC	3 cut(s)	248, 968, 1394
BstNI	CCWGG	3 cut(s)	37, 1220, 1333
BstNSI	RCATGY	2 cut(s)	592, 689
BstSCI	CCNGG	5 cut(s)	35, 302, 849, 1218, 1331
BstSFI	CTRYAG	2 cut(s)	963, 1395
BstSLI	GKGCMC	3 cut(s)	927, 1164, 1449
BstV1I	GCAGC	3 cut(s)	263, 946, 993
BstV2I	GAAGAC	3 cut(s)	357, 368, 1027
BstX2I	RGATCY	2 cut(s)	463, 1454
BstXI	CCANNNNNNTGG	2 cut(s)	649, 1332
BstYI	RGATCY	2 cut(s)	463, 1454
Bsu36I	CCTNAGG	1 cut(s)	1311
BsuI	GTATCC	1 cut(s)	1330
BsuRI	GGCC	7 cut(s)	31, 420, 987, 1071, 1107, 1162, 1308
BtsCI	GGATG	9 cut(s)	862, 953, 1000, 1041, 1093, 1263, 1585, 1623, 1714
BtsI	GCAGTG	1 cut(s)	1278
BtsIMutI	CAGTG	4 cut(s)	229, 250, 564, 1278
BveI	ACCTGC	1 cut(s)	1558
Cac8I	GCNNGC	4 cut(s)	384, 655, 687, 1696
CaiI	CAGNNNCTG	1 cut(s)	1550
CfoI	GCGC	1 cut(s)	1589
Cfr13I	GGNCC	6 cut(s)	306, 418, 1106, 1160, 1161, 1307
Csp6I	GTAC	2 cut(s)	46, 100
CspCI	CAANNNNNGTGG	2 cut(s)	1423, 1458
CviAII	CATG	7 cut(s)	215, 329, 589, 686, 1027, 1166, 1705
CviQI	GTAC	2 cut(s)	46, 100
DdeI	CTNAG	5 cut(s)	42, 459, 558, 1294, 1311
DpnI	GATC	9 cut(s)	72, 327, 465, 726, 780, 942, 1191, 1456, 1518
DpnII	GATC	9 cut(s)	70, 325, 463, 724, 778, 940, 1189, 1454, 1516
DraI	TTTAAA	1 cut(s)	1353
DrdI	GACNNNNNNGTC	1 cut(s)	1029
DseDI	GACNNNNNNGTC	1 cut(s)	1029
EaeI	YGGCCR	2 cut(s)	985, 1069
Eco130I	CCWWGG	1 cut(s)	1083
Eco24I	GRGCYC	1 cut(s)	1164
Eco31I	GGTCTC	2 cut(s)	887, 1501
Eco47I	GGWCC	1 cut(s)	306
Eco47III	AGCGCT	1 cut(s)	1588
Eco57I	CTGAAG	5 cut(s)	167, 380, 1422, 1467, 1533
Eco81I	CCTNAGG	1 cut(s)	1311
Eco88I	CYCGRG	1 cut(s)	1504
EcoO109I	RGGNCCY	2 cut(s)	1161, 1307
EcoRII	CCWGG	3 cut(s)	35, 1218, 1331
EcoT14I	CCWWGG	1 cut(s)	1083
EcoT22I	ATGCAT	1 cut(s)	1390
EcoT38I	GRGCYC	1 cut(s)	1164
ErhI	CCWWGG	1 cut(s)	1083
FaeI	CATG	7 cut(s)	218, 332, 592, 689, 1030, 1169, 1708
FatI	CATG	7 cut(s)	214, 328, 588, 685, 1026, 1165, 1704
FbaI	TGATCA	2 cut(s)	940, 1189
Fnu4HI	GCNGC	4 cut(s)	252, 960, 982, 985
FokI	GGATG	9 cut(s)	869, 960, 1007, 1048, 1100, 1270, 1592, 1610, 1721
FriOI	GRGCYC	1 cut(s)	1164
Fsp4HI	GCNGC	4 cut(s)	252, 960, 982, 985
FspBI	CTAG	4 cut(s)	11, 441, 978, 1599
GlaI	GCGC	1 cut(s)	1588
GluI	GCNGC	4 cut(s)	252, 960, 982, 985
GsaI	CCCAGC	1 cut(s)	1593
HaeII	RGCGCY	1 cut(s)	1590
HaeIII	GGCC	7 cut(s)	31, 420, 987, 1071, 1107, 1162, 1308
HapII	CCGG	4 cut(s)	303, 309, 318, 851
HhaI	GCGC	1 cut(s)	1589
Hin1II	CATG	7 cut(s)	218, 332, 592, 689, 1030, 1169, 1708
Hin6I	GCGC	1 cut(s)	1587
HinP1I	GCGC	1 cut(s)	1587
HincII	GTYRAC	1 cut(s)	16
HindII	GTYRAC	1 cut(s)	16
HinfI	GANTC	8 cut(s)	312, 812, 1030, 1079, 1346, 1424, 1469, 1502
HpaII	CCGG	4 cut(s)	303, 309, 318, 851
Hpy166II	GTNNAC	4 cut(s)	16, 535, 564, 925
Hpy188I	TCNGA	7 cut(s)	505, 724, 1035, 1078, 1120, 1618, 1639
Hpy8I	GTNNAC	4 cut(s)	16, 535, 564, 925
HpyAV	CCTTC	3 cut(s)	477, 1056, 1217
HpyCH4III	ACNGT	9 cut(s)	212, 224, 283, 367, 494, 568, 1672, 1678, 1757
HpyCH4V	TGCA	8 cut(s)	231, 578, 844, 925, 1388, 1397, 1553, 1694
HpyF10VI	GCNNNNNNNGC	3 cut(s)	248, 968, 1394
HpyF3I	CTNAG	5 cut(s)	42, 459, 558, 1294, 1311
Hsp92II	CATG	7 cut(s)	218, 332, 592, 689, 1030, 1169, 1708
HspAI	GCGC	1 cut(s)	1587
Kpn2I	TCCGGA	2 cut(s)	308, 317
KpnI	GGTACC	1 cut(s)	103
Ksp22I	TGATCA	2 cut(s)	940, 1189
Kzo9I	GATC	9 cut(s)	70, 325, 463, 724, 778, 940, 1189, 1454, 1516
LmnI	GCTCC	2 cut(s)	455, 1584
Lsp1109I	GCAGC	3 cut(s)	263, 946, 993
LweI	GCATC	7 cut(s)	229, 985, 1375, 1397, 1562, 1604, 1632
MaeI	CTAG	4 cut(s)	11, 441, 978, 1599
MaeIII	GTNAC	4 cut(s)	331, 436, 1643, 1672
MalI	GATC	9 cut(s)	72, 327, 465, 726, 780, 942, 1191, 1456, 1518
MboI	GATC	9 cut(s)	70, 325, 463, 724, 778, 940, 1189, 1454, 1516
MboII	GAAGA	7 cut(s)	194, 362, 373, 1032, 1107, 1262, 1526
MflI	RGATCY	2 cut(s)	463, 1454
MhlI	GDGCHC	3 cut(s)	927, 1164, 1449
MlsI	TGGCCA	1 cut(s)	1071
MluCI	AATT	8 cut(s)	527, 546, 617, 767, 784, 800, 1136, 1354
MluNI	TGGCCA	1 cut(s)	1071
MlyI	GAGTC	2 cut(s)	1039, 1496
MmeI	TCCRAC	6 cut(s)	48, 893, 1013, 1196, 1395, 1562
Mox20I	TGGCCA	1 cut(s)	1071
Mph1103I	ATGCAT	1 cut(s)	1390
MroI	TCCGGA	2 cut(s)	308, 317
MroXI	GAANNNNTTC	1 cut(s)	1227
MscI	TGGCCA	1 cut(s)	1071
MseI	TTAA	4 cut(s)	645, 1262, 1352, 1734
MslI	CAYNNNNRTG	1 cut(s)	647
Msp20I	TGGCCA	1 cut(s)	1071
MspA1I	CMGCKG	2 cut(s)	932, 984
MspI	CCGG	4 cut(s)	303, 309, 318, 851
MspR9I	CCNGG	5 cut(s)	37, 304, 851, 1220, 1333
MvaI	CCWGG	3 cut(s)	37, 1220, 1333
MwoI	GCNNNNNNNGC	3 cut(s)	248, 968, 1394
NciI	CCSGG	2 cut(s)	304, 851
NdeII	GATC	9 cut(s)	70, 325, 463, 724, 778, 940, 1189, 1454, 1516
NlaIII	CATG	7 cut(s)	218, 332, 592, 689, 1030, 1169, 1708
NlaIV	GGNNCC	9 cut(s)	101, 307, 419, 465, 1044, 1162, 1163, 1456, 1536
NmuCI	GTSAC	2 cut(s)	331, 1643
NsiI	ATGCAT	1 cut(s)	1390
NspI	RCATGY	2 cut(s)	592, 689
PaeI	GCATGC	1 cut(s)	689
PaeR7I	CTCGAG	1 cut(s)	1504
PasI	CCCWGGG	2 cut(s)	36, 1332
PdmI	GAANNNNTTC	1 cut(s)	1227
PfeI	GAWTC	6 cut(s)	312, 812, 1079, 1346, 1424, 1469
PkrI	GCNGC	4 cut(s)	253, 961, 983, 986
PleI	GAGTC	2 cut(s)	1038, 1496
PpsI	GAGTC	2 cut(s)	1038, 1496
Psp6I	CCWGG	3 cut(s)	35, 1218, 1331
PspFI	CCCAGC	1 cut(s)	1589
PspGI	CCWGG	3 cut(s)	35, 1218, 1331
PspN4I	GGNNCC	9 cut(s)	101, 307, 419, 465, 1044, 1162, 1163, 1456, 1536
PspOMI	GGGCCC	1 cut(s)	1160
PspPI	GGNCC	6 cut(s)	306, 418, 1106, 1160, 1161, 1307
PstI	CTGCAG	1 cut(s)	1399
PstNI	CAGNNNCTG	1 cut(s)	1550
PsuI	RGATCY	2 cut(s)	463, 1454
PvuII	CAGCTG	1 cut(s)	932
RsaI	GTAC	2 cut(s)	47, 101
RsaNI	GTAC	2 cut(s)	46, 100
RseI	CAYNNNNRTG	1 cut(s)	647
SaqAI	TTAA	4 cut(s)	645, 1262, 1352, 1734
SatI	GCNGC	4 cut(s)	252, 960, 982, 985
Sau3AI	GATC	9 cut(s)	70, 325, 463, 724, 778, 940, 1189, 1454, 1516
Sau96I	GGNCC	6 cut(s)	306, 418, 1106, 1160, 1161, 1307
SchI	GAGTC	2 cut(s)	1039, 1496
ScrFI	CCNGG	5 cut(s)	37, 304, 851, 1220, 1333
SduI	GDGCHC	3 cut(s)	927, 1164, 1449
SfaNI	GCATC	7 cut(s)	229, 985, 1375, 1397, 1562, 1604, 1632
SfcI	CTRYAG	2 cut(s)	963, 1395
Sfr274I	CTCGAG	1 cut(s)	1504
SinI	GGWCC	1 cut(s)	306
SlaI	CTCGAG	1 cut(s)	1504
SmiMI	CAYNNNNRTG	1 cut(s)	647
SmlI	CTYRAG	1 cut(s)	1504
SmoI	CTYRAG	1 cut(s)	1504
SpeI	ACTAGT	1 cut(s)	440
SphI	GCATGC	1 cut(s)	689
Sse9I	AATT	8 cut(s)	527, 546, 617, 767, 784, 800, 1136, 1354
SsiI	CCGC	3 cut(s)	592, 984, 1328
SspI	AATATT	1 cut(s)	277
SspMI	CTAG	4 cut(s)	11, 441, 978, 1599
StyD4I	CCNGG	5 cut(s)	35, 302, 849, 1218, 1331
StyI	CCWWGG	1 cut(s)	1083
TaaI	ACNGT	9 cut(s)	212, 224, 283, 367, 494, 568, 1672, 1678, 1757
TaqI	TCGA	4 cut(s)	1242, 1460, 1505, 1759
TasI	AATT	8 cut(s)	527, 546, 617, 767, 784, 800, 1136, 1354
TauI	GCSGC	1 cut(s)	987
TfiI	GAWTC	6 cut(s)	312, 812, 1079, 1346, 1424, 1469
Tru1I	TTAA	4 cut(s)	645, 1262, 1352, 1734
Tru9I	TTAA	4 cut(s)	645, 1262, 1352, 1734
TscAI	CASTG	4 cut(s)	229, 250, 571, 1278
TseFI	GTSAC	2 cut(s)	331, 1643
TseI	GCWGC	3 cut(s)	251, 959, 981
Tsp45I	GTSAC	2 cut(s)	331, 1643
TspDTI	ATGAA	6 cut(s)	70, 363, 644, 694, 1047, 1335
TspGWI	ACGGA	2 cut(s)	960, 1001
TspRI	CASTG	4 cut(s)	229, 250, 571, 1278
VneI	GTGCAC	1 cut(s)	923
VpaK11BI	GGWCC	1 cut(s)	306
XapI	RAATTY	5 cut(s)	546, 617, 767, 1136, 1354
XceI	RCATGY	2 cut(s)	592, 689
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1033, 1675
XhoI	CTCGAG	1 cut(s)	1504
XmnI	GAANNNNTTC	1 cut(s)	1227
XspI	CTAG	4 cut(s)	11, 441, 978, 1599
Zsp2I	ATGCAT	1 cut(s)	1390

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.