Rorug04G0156000

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003389
Physical Location & Seq
Forward (+)
27298290 .. 27301245
2956 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0156000.1

Sequence Viewer

Length: 630 bp
ATGCTCTCTCACAATGCACCTTCACGGGAAAGTTCTAGAGAGAGAAACAAAAGCTTAGAGAGAGAGAGAGAGATGGCAGCCTACACCGAATCTTGGGTTTCTTCTTCGATCCAAGAAAACCCAAATGAAGAGGAAGCAAATGAGTCATTGGAATCAATCTTTTCTTCTTCTGCGTTCAACCGACAAGTGGAGCGGGAAGAAGGAGAATTGCCCAAGCTAAGGAGCTCGTCGTCGAGCGGCAGCAGGAAGAAGAAGAAGAAGAACCAGGTTTTGCTTGAAGGGTATGTTGATTCGGCCGATGAGGACGATCTGTCGAGGACCAAGAGCTTGACGGATGAGGATCTCGATGAGCTCAAAGGGTGCTTGGATCTGGGATTTGGGTTCAGCTACGAAGAAATTCCCGAGCTCTGTAACACCTTGCCTGCTCTTGAGCTTTGTTACTCTATGAGCCAGAAGTTCATGGACAAGTCGCCGGAGAGCTCTCCTGCTGCACTGGATTCCTGCTCTTCTGTGTCCAGTCCGATTGCCAATTGGAAGATCTCCAGTCCTGGTGACCATCCAGAGGATGTGAAGGCGAGGCTGAAATACTGGGCACAGGCTGTGGCGTGCACAGTTAGATTGTGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

209

Amino Acids

23.34

Weight (kDa)

4.79

Isoelectric Point (pI)

67.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1685 PF07939 110 - 163 5.5e-25 Protein of unknown function (DUF1685)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 193, 237
AciI CCGC 2 cut(s) 193, 237
AclWI GGATC 3 cut(s) 103, 348, 375
AcoI YGGCCR 1 cut(s) 294
AcsI RAATTY 1 cut(s) 396
AfiI CCNNNNNNNGG 4 cut(s) 93, 187, 219, 562
AgsI TTSAA 2 cut(s) 178, 278
AhdI GACNNNNNGTC 1 cut(s) 310
AjnI CCWGG 2 cut(s) 264, 547
Alw21I GWGCWC 5 cut(s) 227, 354, 408, 482, 611
Alw44I GTGCAC 1 cut(s) 607
AlwI GGATC 3 cut(s) 103, 348, 375
Ama87I CYCGRG 1 cut(s) 401
AoxI GGCC 1 cut(s) 294
ApaLI GTGCAC 1 cut(s) 607
ApeKI GCWGC 4 cut(s) 77, 240, 488, 624
ApoI RAATTY 1 cut(s) 396
Asp700I GAANNNNTTC 1 cut(s) 396
AspS9I GGNCC 1 cut(s) 318
AsuHPI GGTGA 1 cut(s) 563
AvaI CYCGRG 1 cut(s) 401
AvaII GGWCC 1 cut(s) 318
BaeGI GKGCMC 2 cut(s) 595, 611
BanII GRGCYC 4 cut(s) 227, 354, 408, 482
Bbv12I GWGCWC 5 cut(s) 227, 354, 408, 482, 611
BbvI GCAGC 3 cut(s) 89, 252, 475
BccI CCATC 2 cut(s) 67, 564
BciT130I CCWGG 2 cut(s) 266, 549
BfaI CTAG 2 cut(s) 36, 628
BglII AGATCT 1 cut(s) 537
BisI GCNGC 5 cut(s) 78, 238, 241, 489, 625
BlsI GCNGC 5 cut(s) 79, 239, 242, 490, 626
Bme1390I CCNGG 2 cut(s) 266, 549
Bme18I GGWCC 1 cut(s) 318
BmeRI GACNNNNNGTC 1 cut(s) 310
BmeT110I CYCGRG 1 cut(s) 401
BmgT120I GGNCC 1 cut(s) 318
BmrFI CCNGG 2 cut(s) 266, 549
BmrI ACTGGG 1 cut(s) 598
BmuI ACTGGG 1 cut(s) 598
BpmI CTGGAG 1 cut(s) 526
Bpu10I CCTNAGC 1 cut(s) 218
BpuEI CTTGAG 1 cut(s) 449
BsaBI GATNNNNATC 1 cut(s) 339
Bsc4I CCNNNNNNNGG 4 cut(s) 93, 187, 219, 562
Bse1I ACTGG 4 cut(s) 498, 516, 543, 593
Bse8I GATNNNNATC 1 cut(s) 339
BseBI CCWGG 2 cut(s) 266, 549
BseGI GGATG 3 cut(s) 340, 556, 571
BseJI GATNNNNATC 1 cut(s) 339
BseLI CCNNNNNNNGG 4 cut(s) 93, 187, 219, 562
BseNI ACTGG 4 cut(s) 498, 516, 543, 593
BseSI GKGCMC 2 cut(s) 595, 611
BseX3I CGGCCG 1 cut(s) 294
BseXI GCAGC 3 cut(s) 89, 252, 475
BsgI GTGCAG 1 cut(s) 474
Bsh1285I CGRYCG 1 cut(s) 297
BshFI GGCC 1 cut(s) 296
BsiEI CGRYCG 1 cut(s) 297
BsiHKAI GWGCWC 5 cut(s) 227, 354, 408, 482, 611
BsiHKCI CYCGRG 1 cut(s) 401
BsiSI CCGG 1 cut(s) 473
BslI CCNNNNNNNGG 4 cut(s) 93, 187, 219, 562
BsnI GGCC 1 cut(s) 296
BsoBI CYCGRG 1 cut(s) 401
Bsp1286I GDGCHC 6 cut(s) 227, 354, 408, 482, 595, 611
Bsp143I GATC 5 cut(s) 108, 307, 340, 367, 537
BspACI CCGC 2 cut(s) 193, 237
BspANI GGCC 1 cut(s) 296
BspPI GGATC 3 cut(s) 103, 348, 375
BspQI GCTCTTC 1 cut(s) 511
BsrBI CCGCTC 2 cut(s) 193, 237
BsrI ACTGG 4 cut(s) 498, 516, 543, 593
BssMI GATC 5 cut(s) 108, 307, 340, 367, 537
Bst2UI CCWGG 2 cut(s) 266, 549
Bst4CI ACNGT 1 cut(s) 613
Bst6I CTCTTC 2 cut(s) 123, 511
BstC8I GCNNGC 2 cut(s) 423, 607
BstDEI CTNAG 2 cut(s) 55, 218
BstEII GGTNACC 1 cut(s) 551
BstF5I GGATG 3 cut(s) 340, 556, 571
BstKTI GATC 5 cut(s) 111, 310, 343, 370, 540
BstMBI GATC 5 cut(s) 108, 307, 340, 367, 537
BstMCI CGRYCG 1 cut(s) 297
BstNI CCWGG 2 cut(s) 266, 549
BstPI GGTNACC 1 cut(s) 551
BstSCI CCNGG 2 cut(s) 264, 547
BstSLI GKGCMC 2 cut(s) 595, 611
BstV1I GCAGC 3 cut(s) 89, 252, 475
BstX2I RGATCY 3 cut(s) 340, 367, 537
BstYI RGATCY 3 cut(s) 340, 367, 537
BstZI CGGCCG 1 cut(s) 294
BsuRI GGCC 1 cut(s) 296
BtsCI GGATG 3 cut(s) 340, 556, 571
BtsIMutI CAGTG 1 cut(s) 491
Cac8I GCNNGC 2 cut(s) 423, 607
Cfr13I GGNCC 1 cut(s) 318
CsiI ACCWGGT 1 cut(s) 264
CviAII CATG 1 cut(s) 460
DdeI CTNAG 2 cut(s) 55, 218
DpnI GATC 5 cut(s) 110, 309, 342, 369, 539
DpnII GATC 5 cut(s) 108, 307, 340, 367, 537
DriI GACNNNNNGTC 1 cut(s) 310
EaeI YGGCCR 1 cut(s) 294
EagI CGGCCG 1 cut(s) 294
Eam1104I CTCTTC 2 cut(s) 123, 511
Eam1105I GACNNNNNGTC 1 cut(s) 310
EarI CTCTTC 2 cut(s) 123, 511
Ecl136II GAGCTC 4 cut(s) 225, 352, 406, 480
EclXI CGGCCG 1 cut(s) 294
Eco24I GRGCYC 4 cut(s) 227, 354, 408, 482
Eco47I GGWCC 1 cut(s) 318
Eco52I CGGCCG 1 cut(s) 294
Eco53kI GAGCTC 4 cut(s) 225, 352, 406, 480
Eco88I CYCGRG 1 cut(s) 401
Eco91I GGTNACC 1 cut(s) 551
EcoICRI GAGCTC 4 cut(s) 225, 352, 406, 480
EcoO65I GGTNACC 1 cut(s) 551
EcoRII CCWGG 2 cut(s) 264, 547
EcoT38I GRGCYC 4 cut(s) 227, 354, 408, 482
FaeI CATG 1 cut(s) 463
FaiI YATR 3 cut(s) 285, 446, 461
FatI CATG 1 cut(s) 459
FauI CCCGC 1 cut(s) 186
Fnu4HI GCNGC 5 cut(s) 78, 238, 241, 489, 625
FokI GGATG 3 cut(s) 347, 543, 578
FriOI GRGCYC 4 cut(s) 227, 354, 408, 482
Fsp4HI GCNGC 5 cut(s) 78, 238, 241, 489, 625
FspBI CTAG 2 cut(s) 36, 628
GluI GCNGC 5 cut(s) 78, 238, 241, 489, 625
GsuI CTGGAG 1 cut(s) 526
HaeIII GGCC 1 cut(s) 296
HapII CCGG 1 cut(s) 473
Hin1II CATG 1 cut(s) 463
HindIII AAGCTT 1 cut(s) 52
HinfI GANTC 5 cut(s) 89, 143, 152, 290, 497
HpaII CCGG 1 cut(s) 473
HphI GGTGA 1 cut(s) 563
Hpy166II GTNNAC 1 cut(s) 609
Hpy188I TCNGA 1 cut(s) 522
Hpy188III TCNNGA 5 cut(s) 36, 344, 401, 428, 560
Hpy8I GTNNAC 1 cut(s) 609
Hpy99I CGWCG 2 cut(s) 232, 235
HpyAV CCTTC 4 cut(s) 30, 194, 272, 565
HpyCH4III ACNGT 1 cut(s) 613
HpyCH4V TGCA 4 cut(s) 17, 491, 609, 624
HpyF3I CTNAG 2 cut(s) 55, 218
Hsp92II CATG 1 cut(s) 463
Kzo9I GATC 5 cut(s) 108, 307, 340, 367, 537
LguI GCTCTTC 1 cut(s) 511
LmnI GCTCC 2 cut(s) 190, 222
Lsp1109I GCAGC 3 cut(s) 89, 252, 475
MabI ACCWGGT 1 cut(s) 264
MaeI CTAG 2 cut(s) 36, 628
MaeIII GTNAC 3 cut(s) 410, 437, 551
MalI GATC 5 cut(s) 110, 309, 342, 369, 539
MbiI CCGCTC 2 cut(s) 193, 237
MboI GATC 5 cut(s) 108, 307, 340, 367, 537
MfeI CAATTG 1 cut(s) 529
MflI RGATCY 3 cut(s) 340, 367, 537
MhlI GDGCHC 6 cut(s) 227, 354, 408, 482, 595, 611
MluCI AATT 3 cut(s) 206, 396, 529
MlyI GAGTC 1 cut(s) 152
MnlI CCTC 6 cut(s) 124, 295, 309, 331, 556, 570
MroXI GAANNNNTTC 1 cut(s) 396
MspI CCGG 1 cut(s) 473
MspR9I CCNGG 2 cut(s) 266, 549
MunI CAATTG 1 cut(s) 529
MvaI CCWGG 2 cut(s) 266, 549
NdeII GATC 5 cut(s) 108, 307, 340, 367, 537
NlaIII CATG 1 cut(s) 463
NmuCI GTSAC 1 cut(s) 551
PciSI GCTCTTC 1 cut(s) 511
PdmI GAANNNNTTC 1 cut(s) 396
PfeI GAWTC 4 cut(s) 89, 152, 290, 497
PkrI GCNGC 5 cut(s) 79, 239, 242, 490, 626
PleI GAGTC 1 cut(s) 151
PpsI GAGTC 1 cut(s) 151
Psp124BI GAGCTC 4 cut(s) 227, 354, 408, 482
Psp6I CCWGG 2 cut(s) 264, 547
PspEI GGTNACC 1 cut(s) 551
PspGI CCWGG 2 cut(s) 264, 547
PspPI GGNCC 1 cut(s) 318
PsuI RGATCY 3 cut(s) 340, 367, 537
SacI GAGCTC 4 cut(s) 227, 354, 408, 482
SapI GCTCTTC 1 cut(s) 511
SatI GCNGC 5 cut(s) 78, 238, 241, 489, 625
Sau3AI GATC 5 cut(s) 108, 307, 340, 367, 537
Sau96I GGNCC 1 cut(s) 318
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 2 cut(s) 266, 549
SduI GDGCHC 6 cut(s) 227, 354, 408, 482, 595, 611
SexAI ACCWGGT 1 cut(s) 264
SinI GGWCC 1 cut(s) 318
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 3 cut(s) 206, 396, 529
SsiI CCGC 2 cut(s) 193, 237
SspMI CTAG 2 cut(s) 36, 628
SstI GAGCTC 4 cut(s) 227, 354, 408, 482
StyD4I CCNGG 2 cut(s) 264, 547
TaaI ACNGT 1 cut(s) 613
TaqI TCGA 4 cut(s) 107, 233, 314, 345
TasI AATT 3 cut(s) 206, 396, 529
TauI GCSGC 1 cut(s) 240
TfiI GAWTC 4 cut(s) 89, 152, 290, 497
TscAI CASTG 1 cut(s) 498
TseFI GTSAC 1 cut(s) 551
TseI GCWGC 4 cut(s) 77, 240, 488, 624
Tsp45I GTSAC 1 cut(s) 551
TspDTI ATGAA 2 cut(s) 141, 448
TspGWI ACGGA 1 cut(s) 347
TspRI CASTG 1 cut(s) 498
VneI GTGCAC 1 cut(s) 607
VpaK11BI GGWCC 1 cut(s) 318
XapI RAATTY 1 cut(s) 396
XbaI TCTAGA 1 cut(s) 35
XmnI GAANNNNTTC 1 cut(s) 396
XspI CTAG 2 cut(s) 36, 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.