Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003389

Physical Location & Seq

Genomic Coordinates Forward (+)

15566287 .. 15572361

Gene Structure 6075 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1181400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1770 bp

ATGGAGCAGCTTGTCAACTTTATTATTCGGCCTCCCAGGGCTGAATACGACCCAAAAAATGATTTGTTGGATGAAGAGTTCATACTGAAAGGGAAATGGTACCAAAGAAAGGATGTGGAGGTGAAAAATGGTCGGGGAGATGTCCTGAAGTGTAGTCATTACACGCCCATTGTTAGTCCAGAAGAAAAGCCTTTGCCTTGTGTAATATATTGTCATGGAAACAGTGGGTGCAGGGCTGATGCCAGTGAAGCAGCTATTATTTTGCTTCCGTCAAATATTACAGTTTTCACTCTTGATTTCTCAGGATCCGGAATCTCCGGAGGAGATCACGTGACTTTAGGGTGGAATGAAAAGGATGACCTGAAGACTGTGGTTGATTATTTGCGAGCAGATGGAAATGTATCTTTAATTGGCTTGTGGGGCCGTTCAATGGGTGCTGTTACCAGTCTTTTGTATGGAGCTGAGGATCCTTCAATAGCAGGAATGGTTCTTGACAGTCCATTCTCTGATTTGGTTGATTTGATGATGGAATTGGTGGACACCTACAAAATTCGTTTGCCGAAGTTCACTGTGAAGTTTGCAATCCAATACATGCGAAGAGCTATCCAGAAAAAAGCAAAATTTGACATAATGAAATTGAACACAATTAAGGTGGCAAGCTCTTGCTTTGTTCCAGTTTTGTTTGGGCATGCCATTGATGATGATTTCATACAACCCCATCATTCAGATCGTATATTTGAGGCTTATATGGGAGACAAAAATATCATCAAATTTGAGGGAGATCACAATTCTCCACGACCTCAATTCTACTTTGATTCTATAAACATCTTTTTCCACAATGTCTTGCAACCCCCGGAAGATGAGGTTGGGGGAACATTTTTTGACACTCTACACAGTACCTTTGGTAAGGGTACTTGGAGAAGTGTGCACACTGCTGATTATGATCATGGACGTTCAACTGCTTCTGCAGAAGCAGCACCGAGCAGCGGCCAACAGGATGCCATTAGACAACTCCGGTCAAGAAGACCCATGAGTCGGATGGATGTTCCTTCTGGTATTTCATCTACTGATGCTCAATCTGAATCCAAGGATGGAAAAAGCGATAATGGCCCATCATCTTCTGATATGATTAGCTTTGAATTTGCAAATGGCAGTCCTTGTGGGCCCCATGTTCCGACCACGATGGATGATGATCAGTATGTAGAATACCAGCTTGATGACCTGGCAGGTTTTCCAAGTGATGTTGATGAGGAAGAACGGATGTTCATGGAAGCAGTGATTTTGTCTCTAAAAGATTTAGAAATGAGAGGCCCTCAGGCAGAAGAGCAACCACCAGGAGATACCCCGGATTCCTTAAAGTCGTCACAAAAAGATGACCAGCGGGATGATGCATCTCCTGCAGAGAAGCGAGAGCTGTTGGAGACAGAATCTACTTCCACTTCAGTTGGGCACCTTGGATCCTTGAAAACAGAATCTACTTCCACTTCAGTAAGTAAATCCCACAACTCGAGACCTGAAGATCAGTCTCGTACAAGAGTTCCATCCACAGAACCTGCGTTACAAACGCCTCTATCCATAATGGAGAATGGGAGCGCCGGGCCATCTAGCCAAAGCGATACATCAGAAAGCATCCAAAGCACTTCAGATGGTGACTTATCAGCCAAGACGACAGCCACTGTAACGGTGGTAAAAAACCCTGCAAGCAATGTCATGGATGGTTTGATACGTCGATGGGATTTTAACTTCTTTAGAAACAACCACAGCCGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hydrolase_4

Protein Analysis

590

Amino Acids

65.13

Weight (kDa)

4.9

Isoelectric Point (pI)

52.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase_4	PF12146	64 - 175	3.5e-10	Serine aminopeptidase, S33
Abhydrolase_1	PF00561	66 - 182	1.9e-08	alpha/beta hydrolase fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003389)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23540 AT3G23540 AT3G23540 AT4G14290 AT4G14290 AT4G14290 AT4G14290 AT4G14290
fragaria_vesca	FvH4_4g14200 FvH4_4g14200 FvH4_4g14200 FvH4_4g14200 FvH4_4g14200 FvH4_4g14200 FvH4_4g14200
malus_domestica	MD13G1180700.v1.1 MD16G1181400.v1.1
prunus_persica	Prupe.1G148800_v2.0.a1 Prupe.1G148800_v2.0.a1
pyrus_communis	pycom12g09460 pycom12g09470 pycom12g09480 pycom13g15590 pycom16g15220
rosa_chinensis	RchiOBHm_Chr4g0419001
rosa_laevigata	RLG00000007834
rosa_multiflora	Rmu_sc0010159.1_g000002
rosa_roxburghii	Rroxscaffold_5G00362060
rosa_rugosa	Rorug04G0156000 Rorug04G0156000 Rorug04G0156000 Rorug04G0156100
rosa_samantha	Rh4AG217800 Rh4BG212400 Rh4DG216400
rosa_wichuraiana	Rw4G018620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1032
Acc36I	ACCTGC	2 cut(s)	1217, 1561
Acc65I	GGTACC	1 cut(s)	99
AccB1I	GGYRCC	2 cut(s)	99, 1449
AccIII	TCCGGA	2 cut(s)	308, 317
AciI	CCGC	2 cut(s)	987, 1381
AclWI	GGATC	6 cut(s)	300, 313, 461, 474, 1452, 1465
AcoI	YGGCCR	1 cut(s)	988
AcsI	RAATTY	4 cut(s)	549, 620, 770, 1139
AcuI	CTGAAG	6 cut(s)	167, 383, 1425, 1470, 1536, 1626
AcvI	CACGTG	1 cut(s)	331
AfaI	GTAC	4 cut(s)	101, 898, 913, 1531
AfiI	CCNNNNNNNGG	4 cut(s)	109, 430, 986, 1035
AgsI	TTSAA	6 cut(s)	429, 474, 640, 957, 1139, 1465
AjnI	CCWGG	3 cut(s)	35, 1221, 1333
AjuI	GAANNNNNNNTTGG	2 cut(s)	849, 881
AloI	GAACNNNNNNTCC	2 cut(s)	62, 94
AluBI	AGCT	8 cut(s)	10, 254, 461, 602, 660, 1134, 1213, 1414
AluI	AGCT	8 cut(s)	10, 254, 461, 602, 660, 1134, 1213, 1414
Alw21I	GWGCWC	1 cut(s)	930
Alw26I	GTCTC	5 cut(s)	747, 1290, 1415, 1504, 1530
Alw44I	GTGCAC	1 cut(s)	926
AlwI	GGATC	6 cut(s)	300, 313, 461, 474, 1452, 1465
AlwNI	CAGNNNCTG	2 cut(s)	1553, 1676
Ama87I	CYCGRG	1 cut(s)	1507
Aor13HI	TCCGGA	2 cut(s)	308, 317
AoxI	GGCC	7 cut(s)	29, 421, 988, 1108, 1163, 1309, 1598
ApaI	GGGCCC	1 cut(s)	1167
ApaLI	GTGCAC	1 cut(s)	926
ApeKI	GCWGC	4 cut(s)	7, 251, 974, 984
ApoI	RAATTY	4 cut(s)	549, 620, 770, 1139
Asp718I	GGTACC	1 cut(s)	99
AspLEI	GCGC	1 cut(s)	1595
AspS9I	GGNCC	6 cut(s)	421, 1109, 1163, 1164, 1310, 1598
AsuC2I	CCSGG	3 cut(s)	854, 1346, 1597
AsuHPI	GGTGA	2 cut(s)	133, 1661
AvaI	CYCGRG	1 cut(s)	1507
AxyI	CCTNAGG	1 cut(s)	1314
BaeGI	GKGCMC	3 cut(s)	930, 1167, 1452
BamHI	GGATCC	3 cut(s)	305, 466, 1457
BanI	GGYRCC	2 cut(s)	99, 1449
BanII	GRGCYC	1 cut(s)	1167
BarI	GAAGNNNNNNTAC	2 cut(s)	66, 98
BbrPI	CACGTG	1 cut(s)	331
BbsI	GAAGAC	2 cut(s)	371, 1030
Bbv12I	GWGCWC	1 cut(s)	930
BbvCI	CCTCAGC	1 cut(s)	462
BbvI	GCAGC	4 cut(s)	19, 263, 986, 996
BceAI	ACGGC	1 cut(s)	408
BciT130I	CCWGG	3 cut(s)	37, 1223, 1335
BclI	TGATCA	2 cut(s)	943, 1192
BcnI	CCSGG	3 cut(s)	854, 1346, 1597
BcoDI	GTCTC	5 cut(s)	747, 1290, 1415, 1504, 1530
BfaI	CTAG	1 cut(s)	1605
BfmI	CTRYAG	2 cut(s)	966, 1398
BfoI	RGCGCY	1 cut(s)	1596
BfuAI	ACCTGC	2 cut(s)	1217, 1561
BisI	GCNGC	5 cut(s)	8, 252, 975, 985, 988
BlsI	GCNGC	5 cut(s)	9, 253, 976, 986, 989
Bme1390I	CCNGG	6 cut(s)	37, 854, 1223, 1335, 1346, 1597
BmeT110I	CYCGRG	1 cut(s)	1507
BmgT120I	GGNCC	6 cut(s)	421, 1109, 1163, 1164, 1310, 1598
BmiI	GGNNCC	8 cut(s)	101, 307, 422, 468, 1165, 1166, 1451, 1459
BmrFI	CCNGG	6 cut(s)	37, 854, 1223, 1335, 1346, 1597
BmsI	GCATC	6 cut(s)	229, 988, 1060, 1378, 1400, 1638
BpiI	GAAGAC	2 cut(s)	371, 1030
BplI	GAGNNNNNCTC	2 cut(s)	1297, 1329
Bpu10I	CCTNAGC	1 cut(s)	462
BpuMI	CCSGG	3 cut(s)	854, 1346, 1597
BsaAI	YACGTR	1 cut(s)	331
BsaBI	GATNNNNATC	2 cut(s)	942, 1191
BsaI	GGTCTC	1 cut(s)	1504
BsaJI	CCNNGG	6 cut(s)	35, 36, 852, 1086, 1344, 1453
BsaWI	WCCGGW	3 cut(s)	308, 317, 1014
BsaXI	ACNNNNNCTCC	2 cut(s)	315, 345
Bsc4I	CCNNNNNNNGG	4 cut(s)	109, 430, 986, 1035
Bse1I	ACTGG	3 cut(s)	243, 444, 674
Bse21I	CCTNAGG	1 cut(s)	1314
Bse3DI	GCAATG	1 cut(s)	1711
Bse8I	GATNNNNATC	2 cut(s)	942, 1191
BseAI	TCCGGA	2 cut(s)	308, 317
BseBI	CCWGG	3 cut(s)	37, 1223, 1335
BseDI	CCNNGG	6 cut(s)	35, 36, 852, 1086, 1344, 1453
BseJI	GATNNNNATC	2 cut(s)	942, 1191
BseLI	CCNNNNNNNGG	4 cut(s)	109, 430, 986, 1035
BseMI	GCAATG	1 cut(s)	1711
BseMII	CTCAG	3 cut(s)	315, 453, 1328
BseNI	ACTGG	3 cut(s)	243, 444, 674
BseRI	GAGGAG	1 cut(s)	336
BseSI	GKGCMC	3 cut(s)	930, 1167, 1452
BseXI	GCAGC	4 cut(s)	19, 263, 986, 996
BsgI	GTGCAG	1 cut(s)	250
BshFI	GGCC	7 cut(s)	31, 423, 990, 1110, 1165, 1311, 1600
BshNI	GGYRCC	2 cut(s)	99, 1449
BsiHKAI	GWGCWC	1 cut(s)	930
BsiHKCI	CYCGRG	1 cut(s)	1507
BsiSI	CCGG	6 cut(s)	309, 318, 854, 1015, 1346, 1596
BslI	CCNNNNNNNGG	4 cut(s)	109, 430, 986, 1035
BsmAI	GTCTC	5 cut(s)	747, 1290, 1415, 1504, 1530
BsnI	GGCC	7 cut(s)	31, 423, 990, 1110, 1165, 1311, 1600
Bso31I	GGTCTC	1 cut(s)	1504
BsoBI	CYCGRG	1 cut(s)	1507
Bsp120I	GGGCCC	1 cut(s)	1163
Bsp1286I	GDGCHC	3 cut(s)	930, 1167, 1452
Bsp13I	TCCGGA	2 cut(s)	308, 317
Bsp143I	GATC	9 cut(s)	305, 325, 466, 727, 781, 943, 1192, 1457, 1519
BspACI	CCGC	2 cut(s)	987, 1381
BspANI	GGCC	7 cut(s)	31, 423, 990, 1110, 1165, 1311, 1600
BspCNI	CTCAG	3 cut(s)	314, 454, 1327
BspEI	TCCGGA	2 cut(s)	308, 317
BspLI	GGNNCC	8 cut(s)	101, 307, 422, 468, 1165, 1166, 1451, 1459
BspMAI	CTGCAG	2 cut(s)	970, 1402
BspMI	ACCTGC	2 cut(s)	1217, 1561
BspPI	GGATC	6 cut(s)	300, 313, 461, 474, 1452, 1465
BspQI	GCTCTTC	2 cut(s)	592, 1317
BspT107I	GGYRCC	2 cut(s)	99, 1449
BspTNI	GGTCTC	1 cut(s)	1504
BsrDI	GCAATG	1 cut(s)	1711
BsrI	ACTGG	3 cut(s)	243, 444, 674
BssECI	CCNNGG	6 cut(s)	35, 36, 852, 1086, 1344, 1453
BssMI	GATC	9 cut(s)	305, 325, 466, 727, 781, 943, 1192, 1457, 1519
BssT1I	CCWWGG	2 cut(s)	1086, 1453
Bst2UI	CCWGG	3 cut(s)	37, 1223, 1335
Bst4CI	ACNGT	8 cut(s)	224, 283, 370, 497, 571, 896, 1678, 1684
Bst6I	CTCTTC	3 cut(s)	69, 592, 1317
BstAPI	GCANNNNNTGC	1 cut(s)	1397
BstBAI	YACGTR	1 cut(s)	331
BstC8I	GCNNGC	4 cut(s)	387, 658, 690, 1702
BstDEI	CTNAG	3 cut(s)	301, 462, 1314
BstH2I	RGCGCY	1 cut(s)	1596
BstHHI	GCGC	1 cut(s)	1595
BstKTI	GATC	9 cut(s)	308, 328, 469, 730, 784, 946, 1195, 1460, 1522
BstMAI	GTCTC	5 cut(s)	747, 1290, 1415, 1504, 1530
BstMBI	GATC	9 cut(s)	305, 325, 466, 727, 781, 943, 1192, 1457, 1519
BstMWI	GCNNNNNNNGC	6 cut(s)	248, 420, 974, 1107, 1397, 1635
BstNI	CCWGG	3 cut(s)	37, 1223, 1335
BstNSI	RCATGY	2 cut(s)	595, 692
BstSCI	CCNGG	6 cut(s)	35, 852, 1221, 1333, 1344, 1595
BstSFI	CTRYAG	2 cut(s)	966, 1398
BstSLI	GKGCMC	3 cut(s)	930, 1167, 1452
BstV1I	GCAGC	4 cut(s)	19, 263, 986, 996
BstV2I	GAAGAC	2 cut(s)	371, 1030
BstX2I	RGATCY	3 cut(s)	305, 466, 1457
BstYI	RGATCY	3 cut(s)	305, 466, 1457
Bsu36I	CCTNAGG	1 cut(s)	1314
BsuRI	GGCC	7 cut(s)	31, 423, 990, 1110, 1165, 1311, 1600
BtsI	GCAGTG	2 cut(s)	930, 1281
BtsIMutI	CAGTG	6 cut(s)	229, 250, 567, 930, 1281, 1674
BveI	ACCTGC	2 cut(s)	1217, 1561
Cac8I	GCNNGC	4 cut(s)	387, 658, 690, 1702
CaiI	CAGNNNCTG	2 cut(s)	1553, 1676
CfoI	GCGC	1 cut(s)	1595
Cfr13I	GGNCC	6 cut(s)	421, 1109, 1163, 1164, 1310, 1598
Csp6I	GTAC	4 cut(s)	100, 897, 912, 1530
CspCI	CAANNNNNGTGG	4 cut(s)	633, 668, 1426, 1461
CviAII	CATG	8 cut(s)	215, 592, 689, 947, 1030, 1169, 1267, 1711
CviQI	GTAC	4 cut(s)	100, 897, 912, 1530
DdeI	CTNAG	3 cut(s)	301, 462, 1314
DpnI	GATC	9 cut(s)	307, 327, 468, 729, 783, 945, 1194, 1459, 1521
DpnII	GATC	9 cut(s)	305, 325, 466, 727, 781, 943, 1192, 1457, 1519
DrdI	GACNNNNNNGTC	1 cut(s)	1032
DseDI	GACNNNNNNGTC	1 cut(s)	1032
EaeI	YGGCCR	1 cut(s)	988
Eam1104I	CTCTTC	3 cut(s)	69, 592, 1317
EarI	CTCTTC	3 cut(s)	69, 592, 1317
Eco130I	CCWWGG	2 cut(s)	1086, 1453
Eco24I	GRGCYC	1 cut(s)	1167
Eco31I	GGTCTC	1 cut(s)	1504
Eco57I	CTGAAG	6 cut(s)	167, 383, 1425, 1470, 1536, 1626
Eco72I	CACGTG	1 cut(s)	331
Eco81I	CCTNAGG	1 cut(s)	1314
Eco88I	CYCGRG	1 cut(s)	1507
EcoO109I	RGGNCCY	2 cut(s)	1164, 1310
EcoRII	CCWGG	3 cut(s)	35, 1221, 1333
EcoT14I	CCWWGG	2 cut(s)	1086, 1453
EcoT22I	ATGCAT	1 cut(s)	1393
EcoT38I	GRGCYC	1 cut(s)	1167
ErhI	CCWWGG	2 cut(s)	1086, 1453
FaeI	CATG	8 cut(s)	218, 595, 692, 950, 1033, 1172, 1270, 1714
FatI	CATG	8 cut(s)	214, 591, 688, 946, 1029, 1168, 1266, 1710
FauI	CCCGC	1 cut(s)	1374
FbaI	TGATCA	2 cut(s)	943, 1192
Fnu4HI	GCNGC	5 cut(s)	8, 252, 975, 985, 988
FriOI	GRGCYC	1 cut(s)	1167
Fsp4HI	GCNGC	5 cut(s)	8, 252, 975, 985, 988
FspBI	CTAG	1 cut(s)	1605
GlaI	GCGC	1 cut(s)	1594
GluI	GCNGC	5 cut(s)	8, 252, 975, 985, 988
HaeII	RGCGCY	1 cut(s)	1596
HaeIII	GGCC	7 cut(s)	31, 423, 990, 1110, 1165, 1311, 1600
HapII	CCGG	6 cut(s)	309, 318, 854, 1015, 1346, 1596
HhaI	GCGC	1 cut(s)	1595
Hin1II	CATG	8 cut(s)	218, 595, 692, 950, 1033, 1172, 1270, 1714
Hin6I	GCGC	1 cut(s)	1593
HinP1I	GCGC	1 cut(s)	1593
HincII	GTYRAC	1 cut(s)	16
HindII	GTYRAC	1 cut(s)	16
HinfI	GANTC	7 cut(s)	312, 815, 1033, 1082, 1349, 1427, 1472
HpaII	CCGG	6 cut(s)	309, 318, 854, 1015, 1346, 1596
HphI	GGTGA	2 cut(s)	133, 1661
Hpy166II	GTNNAC	4 cut(s)	16, 538, 567, 928
Hpy188I	TCNGA	8 cut(s)	508, 727, 1038, 1081, 1123, 1176, 1624, 1645
Hpy8I	GTNNAC	4 cut(s)	16, 538, 567, 928
Hpy99I	CGWCG	1 cut(s)	1731
HpyAV	CCTTC	2 cut(s)	480, 1059
HpyCH4III	ACNGT	8 cut(s)	224, 283, 370, 497, 571, 896, 1678, 1684
HpyCH4IV	ACGT	3 cut(s)	330, 952, 1726
HpyCH4V	TGCA	9 cut(s)	231, 581, 847, 928, 968, 1145, 1391, 1400, 1700
HpyF10VI	GCNNNNNNNGC	6 cut(s)	248, 420, 974, 1107, 1397, 1635
HpyF3I	CTNAG	3 cut(s)	301, 462, 1314
HpySE526I	ACGT	3 cut(s)	330, 952, 1726
Hsp92II	CATG	8 cut(s)	218, 595, 692, 950, 1033, 1172, 1270, 1714
HspAI	GCGC	1 cut(s)	1593
Kpn2I	TCCGGA	2 cut(s)	308, 317
KpnI	GGTACC	1 cut(s)	103
Ksp22I	TGATCA	2 cut(s)	943, 1192
Kzo9I	GATC	9 cut(s)	305, 325, 466, 727, 781, 943, 1192, 1457, 1519
LguI	GCTCTTC	2 cut(s)	592, 1317
LmnI	GCTCC	3 cut(s)	4, 458, 1590
Lsp1109I	GCAGC	4 cut(s)	19, 263, 986, 996
LweI	GCATC	6 cut(s)	229, 988, 1060, 1378, 1400, 1638
MaeI	CTAG	1 cut(s)	1605
MaeII	ACGT	3 cut(s)	330, 952, 1726
MaeIII	GTNAC	6 cut(s)	331, 439, 1362, 1557, 1649, 1678
MalI	GATC	9 cut(s)	307, 327, 468, 729, 783, 945, 1194, 1459, 1521
MboI	GATC	9 cut(s)	305, 325, 466, 727, 781, 943, 1192, 1457, 1519
MflI	RGATCY	3 cut(s)	305, 466, 1457
MhlI	GDGCHC	3 cut(s)	930, 1167, 1452
MlyI	GAGTC	1 cut(s)	1042
MmeI	TCCRAC	4 cut(s)	48, 1016, 1199, 1398
Mph1103I	ATGCAT	1 cut(s)	1393
MroI	TCCGGA	2 cut(s)	308, 317
MseI	TTAA	4 cut(s)	407, 648, 1355, 1740
MspA1I	CMGCKG	2 cut(s)	987, 1381
MspI	CCGG	6 cut(s)	309, 318, 854, 1015, 1346, 1596
MspR9I	CCNGG	6 cut(s)	37, 854, 1223, 1335, 1346, 1597
MvaI	CCWGG	3 cut(s)	37, 1223, 1335
MwoI	GCNNNNNNNGC	6 cut(s)	248, 420, 974, 1107, 1397, 1635
NciI	CCSGG	3 cut(s)	854, 1346, 1597
NdeII	GATC	9 cut(s)	305, 325, 466, 727, 781, 943, 1192, 1457, 1519
NlaIII	CATG	8 cut(s)	218, 595, 692, 950, 1033, 1172, 1270, 1714
NlaIV	GGNNCC	8 cut(s)	101, 307, 422, 468, 1165, 1166, 1451, 1459
NmuCI	GTSAC	3 cut(s)	331, 1362, 1649
NsiI	ATGCAT	1 cut(s)	1393
NspI	RCATGY	2 cut(s)	595, 692
PaeI	GCATGC	1 cut(s)	692
PaeR7I	CTCGAG	1 cut(s)	1507
PasI	CCCWGGG	1 cut(s)	36
PciSI	GCTCTTC	2 cut(s)	592, 1317
PfeI	GAWTC	6 cut(s)	312, 815, 1082, 1349, 1427, 1472
PkrI	GCNGC	5 cut(s)	9, 253, 976, 986, 989
PleI	GAGTC	1 cut(s)	1041
PmaCI	CACGTG	1 cut(s)	331
PmlI	CACGTG	1 cut(s)	331
PpsI	GAGTC	1 cut(s)	1041
Ppu21I	YACGTR	1 cut(s)	331
Psp6I	CCWGG	3 cut(s)	35, 1221, 1333
PspCI	CACGTG	1 cut(s)	331
PspGI	CCWGG	3 cut(s)	35, 1221, 1333
PspN4I	GGNNCC	8 cut(s)	101, 307, 422, 468, 1165, 1166, 1451, 1459
PspOMI	GGGCCC	1 cut(s)	1163
PspPI	GGNCC	6 cut(s)	421, 1109, 1163, 1164, 1310, 1598
PsrI	GAACNNNNNNTAC	2 cut(s)	1542, 1574
PstI	CTGCAG	2 cut(s)	970, 1402
PstNI	CAGNNNCTG	2 cut(s)	1553, 1676
PsuI	RGATCY	3 cut(s)	305, 466, 1457
RsaI	GTAC	4 cut(s)	101, 898, 913, 1531
RsaNI	GTAC	4 cut(s)	100, 897, 912, 1530
SapI	GCTCTTC	2 cut(s)	592, 1317
SaqAI	TTAA	4 cut(s)	407, 648, 1355, 1740
SatI	GCNGC	5 cut(s)	8, 252, 975, 985, 988
Sau3AI	GATC	9 cut(s)	305, 325, 466, 727, 781, 943, 1192, 1457, 1519
Sau96I	GGNCC	6 cut(s)	421, 1109, 1163, 1164, 1310, 1598
SchI	GAGTC	1 cut(s)	1042
ScrFI	CCNGG	6 cut(s)	37, 854, 1223, 1335, 1346, 1597
SduI	GDGCHC	3 cut(s)	930, 1167, 1452
SfaNI	GCATC	6 cut(s)	229, 988, 1060, 1378, 1400, 1638
SfcI	CTRYAG	2 cut(s)	966, 1398
Sfr274I	CTCGAG	1 cut(s)	1507
SlaI	CTCGAG	1 cut(s)	1507
SmlI	CTYRAG	1 cut(s)	1507
SmoI	CTYRAG	1 cut(s)	1507
SphI	GCATGC	1 cut(s)	692
SsiI	CCGC	2 cut(s)	987, 1381
SspI	AATATT	1 cut(s)	277
SspMI	CTAG	1 cut(s)	1605
StyD4I	CCNGG	6 cut(s)	35, 852, 1221, 1333, 1344, 1595
StyI	CCWWGG	2 cut(s)	1086, 1453
TaaI	ACNGT	8 cut(s)	224, 283, 370, 497, 571, 896, 1678, 1684
TaiI	ACGT	3 cut(s)	333, 955, 1729
TaqI	TCGA	2 cut(s)	1508, 1729
TauI	GCSGC	1 cut(s)	990
TfiI	GAWTC	6 cut(s)	312, 815, 1082, 1349, 1427, 1472
Tru1I	TTAA	4 cut(s)	407, 648, 1355, 1740
Tru9I	TTAA	4 cut(s)	407, 648, 1355, 1740
TscAI	CASTG	6 cut(s)	229, 250, 574, 937, 1281, 1681
TseFI	GTSAC	3 cut(s)	331, 1362, 1649
TseI	GCWGC	4 cut(s)	7, 251, 974, 984
Tsp45I	GTSAC	3 cut(s)	331, 1362, 1649
TspDTI	ATGAA	7 cut(s)	70, 87, 363, 647, 697, 1050, 1255
TspGWI	ACGGA	2 cut(s)	258, 1273
TspRI	CASTG	6 cut(s)	229, 250, 574, 937, 1281, 1681
VneI	GTGCAC	1 cut(s)	926
XapI	RAATTY	4 cut(s)	549, 620, 770, 1139
XceI	RCATGY	2 cut(s)	595, 692
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1036, 1681
XhoI	CTCGAG	1 cut(s)	1507
XspI	CTAG	1 cut(s)	1605
Zsp2I	ATGCAT	1 cut(s)	1393

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.