Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0003389

Physical Location & Seq

Genomic Coordinates Reverse (-)

41328481 .. 41336118

Gene Structure 7638 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G018620.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1557 bp

ATGGAGCAGCTTGTCAACTTCATCATTCGGCCTCCTAGGGCTGAATACGACCCAAAAAATGATTTATTGGACGAGGAGTTCATACTCAAGGGGAAATGGTATCAAAGGAAGGATGTGGAGGTTAAAAACAGTCGGGGAGATGTCCTCAAGTGTAGTCATTACATCCCCATTGTTAGTCCTGAAGGAAAGCCTTTGCCCTGTGTCATATATTGTCATGGAAACAGTGGGTGCAGGGCTGATGCAAGTGAAGCGGCCATTATTTTGCTGCCCTCAAATATTACAGTTTTCGCTCTTGATTTCTCAGGATCTGGGCTCTCTGGTGGAGACCACGTCACATTGGGGTGGAATGAAAAGGATGACTTGAAGACTGTAGTTGATTATTTGCGAGCAGATGGAAATGTCTCTTTAATTGGCCTGTGGGGCCGTTCAATGGGTGCTGTCACGAGTCTCTTGTATGGAGCTGAGGACCCTTCAATCGCAGGAGTTGTTCTTGACAGTCCATTCTCTGATTTGGTTGATTTAATGATGGAACTTGTGGACACCTACAAAATTCGTTTACCGAAGTTCACTGTGAAGTTTGCTATCCAATACATGCGAAGAGCTATCCAGAAAAAGGCAAAATTTGACATAACGAACCTGAACACAATTAAGGTGGCAAGCTCCTGCTTTGTTCCAGCTTTGTTTGGACATGCCATTGAGGACGATTTTATACAACCCCATCATTCAGATCGTATATTTGAGGCTTATATGGGAGATAAAAATATCATCAAGTTTGAGGGAGATCACAACTCTCCACGACCTCAGTTCTACTTTGATTCTATAAATATCTTTTTCCACAATGTTTTGCAACCCCCAGAGGATGAGGTTGGGGGTACGTTTTTCGACACCATGCATGATAACTTCGGCAAGGGTAGTTGGAGAACCGTGCGTGAGGTTGATTATGACCACGGAAGTGTAACTGCTTCTTCTAAAGAACCAGCAACGAGTGGCAGCACACAGGATGCCATTAGCCAACTCCGCTCAAGAAGACCCATGAGTCGAACAGATGTTCCTTCTGGTGTATCATCTAAAGATGACGAATCTCATTCTAAGGATGGAAAAGTCGATCATGATCCTTGTCCATCATCTTCTGACATGATTAGCTTTGAATTCTCAAATGGACACCCCCATGTTCCAGCGCCAATGGACAATGATCAGTATGTGGAATACCAACTTGAGGACTTGGCAGGCTTTCCTAGTGATGTGGAGGAGGAAGAAAGGATGTTCATGGAAGCAGTGATATTGTCACTAAAGGACTTAGAAATGAGACACCCAAACCCACAGGCAGAAGAGCAACCACCAGGGAATACCCCCGATTCTTCAAAGATATCAGAAAAAGATGACTATCATGATGCCGTGTCTCCGGTAGAACAGCGAGAACCTGTAGAGACAGCATCAACTTCCGCATCAAGTGCACATCTGGGTCCTCGAAAGCAGAATCTATTTCCAGTTCAGCTGTTAAGGTCGATGATTCGAGACCTGAGCAGCAAATTCCTGACGCAGCTGTACCATCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hydrolase_4

Protein Analysis

518

Amino Acids

57.97

Weight (kDa)

5.01

Isoelectric Point (pI)

49.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase_4	PF12146	64 - 175	6e-11	Serine aminopeptidase, S33
Abhydrolase_1	PF00561	66 - 187	1.6e-08	alpha/beta hydrolase fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003389)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23540 AT3G23540 AT3G23540 AT4G14290 AT4G14290 AT4G14290 AT4G14290 AT4G14290
fragaria_vesca	FvH4_4g14200 FvH4_4g14200 FvH4_4g14200 FvH4_4g14200 FvH4_4g14200 FvH4_4g14200 FvH4_4g14200
malus_domestica	MD13G1180700.v1.1 MD16G1181400.v1.1
prunus_persica	Prupe.1G148800_v2.0.a1 Prupe.1G148800_v2.0.a1
pyrus_communis	pycom12g09460 pycom12g09470 pycom12g09480 pycom13g15590 pycom16g15220
rosa_chinensis	RchiOBHm_Chr4g0419001
rosa_laevigata	RLG00000007834
rosa_multiflora	Rmu_sc0010159.1_g000002
rosa_roxburghii	Rroxscaffold_5G00362060
rosa_rugosa	Rorug04G0156000 Rorug04G0156000 Rorug04G0156000 Rorug04G0156100
rosa_samantha	Rh4AG217800 Rh4BG212400 Rh4DG216400
rosa_wichuraiana	Rw4G018620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1035
AccBSI	CCGCTC	1 cut(s)	1020
AciI	CCGC	3 cut(s)	251, 1018, 1443
AclWI	GGATC	2 cut(s)	313, 1106
AcoI	YGGCCR	1 cut(s)	252
AcsI	RAATTY	4 cut(s)	549, 620, 1148, 1529
AcuI	CTGAAG	1 cut(s)	201
AfaI	GTAC	2 cut(s)	874, 1547
AfiI	CCNNNNNNNGG	3 cut(s)	430, 613, 1216
AgsI	TTSAA	5 cut(s)	364, 429, 474, 1148, 1362
AjiI	CACGTC	1 cut(s)	331
AjnI	CCWGG	1 cut(s)	1339
AleI	CACNNNNGTG	1 cut(s)	951
AloI	GAACNNNNNNTCC	4 cut(s)	62, 94, 1033, 1065
AluBI	AGCT	8 cut(s)	10, 461, 602, 660, 677, 1143, 1495, 1543
AluI	AGCT	8 cut(s)	10, 461, 602, 660, 677, 1143, 1495, 1543
Alw21I	GWGCWC	1 cut(s)	1456
Alw26I	GTCTC	7 cut(s)	318, 406, 452, 1300, 1404, 1421, 1509
Alw44I	GTGCAC	1 cut(s)	1452
AlwI	GGATC	2 cut(s)	313, 1106
AlwNI	CAGNNNCTG	1 cut(s)	308
AoxI	GGCC	4 cut(s)	29, 252, 412, 421
ApaLI	GTGCAC	1 cut(s)	1452
ApeKI	GCWGC	5 cut(s)	7, 265, 990, 1524, 1540
ApoI	RAATTY	4 cut(s)	549, 620, 1148, 1529
AspA2I	CCTAGG	1 cut(s)	35
AspLEI	GCGC	1 cut(s)	1180
AspS9I	GGNCC	3 cut(s)	421, 466, 1463
AvaII	GGWCC	2 cut(s)	466, 1463
AvrII	CCTAGG	1 cut(s)	35
BaeGI	GKGCMC	1 cut(s)	1456
BanII	GRGCYC	1 cut(s)	315
BauI	CACGAG	1 cut(s)	442
BbsI	GAAGAC	2 cut(s)	371, 1033
Bbv12I	GWGCWC	1 cut(s)	1456
BbvCI	CCTCAGC	1 cut(s)	462
BbvI	GCAGC	5 cut(s)	19, 252, 1002, 1536, 1552
BccI	CCATC	5 cut(s)	386, 520, 726, 1088, 1129
BceAI	ACGGC	2 cut(s)	408, 1379
BciT130I	CCWGG	1 cut(s)	1341
BclI	TGATCA	1 cut(s)	1192
BcoDI	GTCTC	7 cut(s)	318, 406, 452, 1300, 1404, 1421, 1509
BfaI	CTAG	2 cut(s)	36, 1236
BfmI	CTRYAG	2 cut(s)	369, 1422
BfoI	RGCGCY	1 cut(s)	1181
BglI	GCCNNNNNGGC	1 cut(s)	420
BisI	GCNGC	6 cut(s)	8, 252, 266, 991, 1525, 1541
BlnI	CCTAGG	1 cut(s)	35
BlsI	GCNGC	6 cut(s)	9, 253, 267, 992, 1526, 1542
Bme1390I	CCNGG	1 cut(s)	1341
Bme18I	GGWCC	2 cut(s)	466, 1463
BmgBI	CACGTC	1 cut(s)	331
BmgT120I	GGNCC	3 cut(s)	421, 466, 1463
BmiI	GGNNCC	3 cut(s)	422, 468, 1464
BmrFI	CCNGG	1 cut(s)	1341
BmsI	GCATC	5 cut(s)	229, 991, 1381, 1442, 1454
BpiI	GAAGAC	2 cut(s)	371, 1033
BplI	GAGNNNNNCTC	2 cut(s)	129, 161
Bpu10I	CCTNAGC	2 cut(s)	462, 1520
BpuEI	CTTGAG	4 cut(s)	71, 131, 1006, 1235
BsaBI	GATNNNNATC	2 cut(s)	1110, 1383
BsaI	GGTCTC	2 cut(s)	318, 1509
BsaJI	CCNNGG	3 cut(s)	35, 946, 1340
BsaWI	WCCGGW	1 cut(s)	1402
BsaXI	ACNNNNNCTCC	2 cut(s)	315, 345
Bsc4I	CCNNNNNNNGG	3 cut(s)	430, 613, 1216
Bse1I	ACTGG	1 cut(s)	1487
Bse8I	GATNNNNATC	2 cut(s)	1110, 1383
BseBI	CCWGG	1 cut(s)	1341
BseDI	CCNNGG	3 cut(s)	35, 946, 1340
BseGI	GGATG	7 cut(s)	118, 162, 361, 865, 1006, 1099, 1266
BseJI	GATNNNNATC	2 cut(s)	1110, 1383
BseLI	CCNNNNNNNGG	3 cut(s)	430, 613, 1216
BseMII	CTCAG	4 cut(s)	315, 453, 815, 1511
BseNI	ACTGG	1 cut(s)	1487
BseRI	GAGGAG	2 cut(s)	89, 1262
BseSI	GKGCMC	1 cut(s)	1456
BseXI	GCAGC	5 cut(s)	19, 252, 1002, 1536, 1552
BsgI	GTGCAG	1 cut(s)	250
BshFI	GGCC	4 cut(s)	31, 254, 414, 423
BsiHKAI	GWGCWC	1 cut(s)	1456
BsiSI	CCGG	1 cut(s)	1403
BslI	CCNNNNNNNGG	3 cut(s)	430, 613, 1216
BsmAI	GTCTC	7 cut(s)	318, 406, 452, 1300, 1404, 1421, 1509
BsnI	GGCC	4 cut(s)	31, 254, 414, 423
Bso31I	GGTCTC	2 cut(s)	318, 1509
Bsp1286I	GDGCHC	2 cut(s)	315, 1456
Bsp143I	GATC	6 cut(s)	305, 727, 781, 1105, 1111, 1192
BspACI	CCGC	3 cut(s)	251, 1018, 1443
BspANI	GGCC	4 cut(s)	31, 254, 414, 423
BspCNI	CTCAG	4 cut(s)	314, 454, 814, 1512
BspHI	TCATGA	2 cut(s)	1108, 1387
BspLI	GGNNCC	3 cut(s)	422, 468, 1464
BspPI	GGATC	2 cut(s)	313, 1106
BspQI	GCTCTTC	2 cut(s)	592, 1323
BspTNI	GGTCTC	2 cut(s)	318, 1509
BsrBI	CCGCTC	1 cut(s)	1020
BsrI	ACTGG	1 cut(s)	1487
BssECI	CCNNGG	3 cut(s)	35, 946, 1340
BssMI	GATC	6 cut(s)	305, 727, 781, 1105, 1111, 1192
BssSI	CACGAG	1 cut(s)	442
BssT1I	CCWWGG	1 cut(s)	35
Bst2BI	CACGAG	1 cut(s)	442
Bst2UI	CCWGG	1 cut(s)	1341
Bst4CI	ACNGT	7 cut(s)	131, 224, 283, 370, 497, 571, 925
Bst6I	CTCTTC	2 cut(s)	592, 1323
BstAPI	GCANNNNNTGC	1 cut(s)	1451
BstC8I	GCNNGC	3 cut(s)	387, 658, 1228
BstDEI	CTNAG	6 cut(s)	301, 462, 801, 1089, 1297, 1520
BstDSI	CCRYGG	1 cut(s)	946
BstF5I	GGATG	7 cut(s)	118, 162, 361, 865, 1006, 1099, 1266
BstH2I	RGCGCY	1 cut(s)	1181
BstHHI	GCGC	1 cut(s)	1180
BstKTI	GATC	6 cut(s)	308, 730, 784, 1108, 1114, 1195
BstMAI	GTCTC	7 cut(s)	318, 406, 452, 1300, 1404, 1421, 1509
BstMBI	GATC	6 cut(s)	305, 727, 781, 1105, 1111, 1192
BstMWI	GCNNNNNNNGC	4 cut(s)	248, 420, 1017, 1451
BstNI	CCWGG	1 cut(s)	1341
BstNSI	RCATGY	2 cut(s)	595, 692
BstSCI	CCNGG	1 cut(s)	1339
BstSFI	CTRYAG	2 cut(s)	369, 1422
BstSLI	GKGCMC	1 cut(s)	1456
BstV1I	GCAGC	5 cut(s)	19, 252, 1002, 1536, 1552
BstV2I	GAAGAC	2 cut(s)	371, 1033
BstX2I	RGATCY	1 cut(s)	305
BstYI	RGATCY	1 cut(s)	305
BsuRI	GGCC	4 cut(s)	31, 254, 414, 423
BtgI	CCRYGG	1 cut(s)	946
BtrI	CACGTC	1 cut(s)	331
BtsCI	GGATG	7 cut(s)	118, 162, 361, 865, 1006, 1099, 1266
BtsI	GCAGTG	1 cut(s)	1281
BtsIMutI	CAGTG	3 cut(s)	229, 567, 1281
Cac8I	GCNNGC	3 cut(s)	387, 658, 1228
CaiI	CAGNNNCTG	1 cut(s)	308
CciI	TCATGA	2 cut(s)	1108, 1387
CfoI	GCGC	1 cut(s)	1180
Cfr13I	GGNCC	3 cut(s)	421, 466, 1463
CseI	GACGC	1 cut(s)	1546
Csp6I	GTAC	2 cut(s)	873, 1546
CspCI	CAANNNNNGTGG	2 cut(s)	633, 668
CviQI	GTAC	2 cut(s)	873, 1546
DdeI	CTNAG	6 cut(s)	301, 462, 801, 1089, 1297, 1520
DpnI	GATC	6 cut(s)	307, 729, 783, 1107, 1113, 1194
DpnII	GATC	6 cut(s)	305, 727, 781, 1105, 1111, 1192
DrdI	GACNNNNNNGTC	1 cut(s)	1035
DseDI	GACNNNNNNGTC	1 cut(s)	1035
EaeI	YGGCCR	1 cut(s)	252
Eam1104I	CTCTTC	2 cut(s)	592, 1323
EarI	CTCTTC	2 cut(s)	592, 1323
Eco130I	CCWWGG	1 cut(s)	35
Eco24I	GRGCYC	1 cut(s)	315
Eco31I	GGTCTC	2 cut(s)	318, 1509
Eco32I	GATATC	1 cut(s)	1368
Eco47I	GGWCC	2 cut(s)	466, 1463
Eco57I	CTGAAG	1 cut(s)	201
EcoO109I	RGGNCCY	2 cut(s)	466, 1463
EcoRI	GAATTC	1 cut(s)	1148
EcoRII	CCWGG	1 cut(s)	1339
EcoRV	GATATC	1 cut(s)	1368
EcoT14I	CCWWGG	1 cut(s)	35
EcoT22I	ATGCAT	1 cut(s)	894
EcoT38I	GRGCYC	1 cut(s)	315
ErhI	CCWWGG	1 cut(s)	35
FalI	AAGNNNNNCTT	2 cut(s)	344, 376
FbaI	TGATCA	1 cut(s)	1192
Fnu4HI	GCNGC	6 cut(s)	8, 252, 266, 991, 1525, 1541
FokI	GGATG	7 cut(s)	125, 149, 368, 872, 1013, 1106, 1273
FriOI	GRGCYC	1 cut(s)	315
Fsp4HI	GCNGC	6 cut(s)	8, 252, 266, 991, 1525, 1541
FspBI	CTAG	2 cut(s)	36, 1236
GlaI	GCGC	1 cut(s)	1179
GluI	GCNGC	6 cut(s)	8, 252, 266, 991, 1525, 1541
HaeII	RGCGCY	1 cut(s)	1181
HaeIII	GGCC	4 cut(s)	31, 254, 414, 423
HapII	CCGG	1 cut(s)	1403
HgaI	GACGC	1 cut(s)	1546
HhaI	GCGC	1 cut(s)	1180
Hin6I	GCGC	1 cut(s)	1178
HinP1I	GCGC	1 cut(s)	1178
HincII	GTYRAC	1 cut(s)	16
HindII	GTYRAC	1 cut(s)	16
HinfI	GANTC	7 cut(s)	445, 815, 1036, 1079, 1355, 1477, 1510
HpaII	CCGG	1 cut(s)	1403
Hpy166II	GTNNAC	5 cut(s)	16, 538, 557, 567, 1454
Hpy188I	TCNGA	4 cut(s)	508, 727, 1132, 1372
Hpy8I	GTNNAC	5 cut(s)	16, 538, 557, 567, 1454
HpyAV	CCTTC	4 cut(s)	103, 176, 480, 1062
HpyCH4III	ACNGT	7 cut(s)	131, 224, 283, 370, 497, 571, 925
HpyCH4IV	ACGT	2 cut(s)	330, 875
HpyCH4V	TGCA	5 cut(s)	231, 242, 847, 892, 1454
HpyF10VI	GCNNNNNNNGC	4 cut(s)	248, 420, 1017, 1451
HpyF3I	CTNAG	6 cut(s)	301, 462, 801, 1089, 1297, 1520
HpySE526I	ACGT	2 cut(s)	330, 875
HspAI	GCGC	1 cut(s)	1178
Ksp22I	TGATCA	1 cut(s)	1192
Kzo9I	GATC	6 cut(s)	305, 727, 781, 1105, 1111, 1192
LguI	GCTCTTC	2 cut(s)	592, 1323
LmnI	GCTCC	3 cut(s)	4, 458, 665
Lsp1109I	GCAGC	5 cut(s)	19, 252, 1002, 1536, 1552
LweI	GCATC	5 cut(s)	229, 991, 1381, 1442, 1454
MaeI	CTAG	2 cut(s)	36, 1236
MaeII	ACGT	2 cut(s)	330, 875
MaeIII	GTNAC	4 cut(s)	331, 439, 955, 1284
MalI	GATC	6 cut(s)	307, 729, 783, 1107, 1113, 1194
MbiI	CCGCTC	1 cut(s)	1020
MboI	GATC	6 cut(s)	305, 727, 781, 1105, 1111, 1192
MboII	GAAGA	8 cut(s)	376, 609, 957, 1038, 1119, 1265, 1340, 1350
MflI	RGATCY	1 cut(s)	305
MhlI	GDGCHC	2 cut(s)	315, 1456
MluCI	AATT	6 cut(s)	408, 549, 620, 645, 1148, 1529
MlyI	GAGTC	2 cut(s)	454, 1045
MmeI	TCCRAC	1 cut(s)	896
Mph1103I	ATGCAT	1 cut(s)	894
MseI	TTAA	5 cut(s)	123, 407, 521, 648, 1499
MslI	CAYNNNNRTG	3 cut(s)	340, 951, 1167
MspA1I	CMGCKG	2 cut(s)	1495, 1543
MspI	CCGG	1 cut(s)	1403
MspR9I	CCNGG	1 cut(s)	1341
MvaI	CCWGG	1 cut(s)	1341
MwoI	GCNNNNNNNGC	4 cut(s)	248, 420, 1017, 1451
NdeII	GATC	6 cut(s)	305, 727, 781, 1105, 1111, 1192
NlaIV	GGNNCC	3 cut(s)	422, 468, 1464
NmuCI	GTSAC	3 cut(s)	331, 439, 1284
NsiI	ATGCAT	1 cut(s)	894
NspI	RCATGY	2 cut(s)	595, 692
OliI	CACNNNNGTG	1 cut(s)	951
PagI	TCATGA	2 cut(s)	1108, 1387
PciSI	GCTCTTC	2 cut(s)	592, 1323
PfeI	GAWTC	5 cut(s)	815, 1079, 1355, 1477, 1510
PflFI	GACNNNGTC	1 cut(s)	329
PkrI	GCNGC	6 cut(s)	9, 253, 267, 992, 1526, 1542
PleI	GAGTC	2 cut(s)	453, 1044
PpsI	GAGTC	2 cut(s)	453, 1044
PpuMI	RGGWCCY	2 cut(s)	466, 1463
Psp5II	RGGWCCY	2 cut(s)	466, 1463
Psp6I	CCWGG	1 cut(s)	1339
PspGI	CCWGG	1 cut(s)	1339
PspN4I	GGNNCC	3 cut(s)	422, 468, 1464
PspPI	GGNCC	3 cut(s)	421, 466, 1463
PspPPI	RGGWCCY	2 cut(s)	466, 1463
PstNI	CAGNNNCTG	1 cut(s)	308
PsuI	RGATCY	1 cut(s)	305
PsyI	GACNNNGTC	1 cut(s)	329
PvuII	CAGCTG	2 cut(s)	1495, 1543
RsaI	GTAC	2 cut(s)	874, 1547
RsaNI	GTAC	2 cut(s)	873, 1546
RseI	CAYNNNNRTG	3 cut(s)	340, 951, 1167
SapI	GCTCTTC	2 cut(s)	592, 1323
SaqAI	TTAA	5 cut(s)	123, 407, 521, 648, 1499
SatI	GCNGC	6 cut(s)	8, 252, 266, 991, 1525, 1541
Sau3AI	GATC	6 cut(s)	305, 727, 781, 1105, 1111, 1192
Sau96I	GGNCC	3 cut(s)	421, 466, 1463
SchI	GAGTC	2 cut(s)	454, 1045
ScrFI	CCNGG	1 cut(s)	1341
SduI	GDGCHC	2 cut(s)	315, 1456
SfaNI	GCATC	5 cut(s)	229, 991, 1381, 1442, 1454
SfcI	CTRYAG	2 cut(s)	369, 1422
SfiI	GGCCNNNNNGGCC	1 cut(s)	420
SinI	GGWCC	2 cut(s)	466, 1463
SmiMI	CAYNNNNRTG	3 cut(s)	340, 951, 1167
SmlI	CTYRAG	4 cut(s)	86, 146, 1021, 1214
SmoI	CTYRAG	4 cut(s)	86, 146, 1021, 1214
Sse9I	AATT	6 cut(s)	408, 549, 620, 645, 1148, 1529
SsiI	CCGC	3 cut(s)	251, 1018, 1443
SspI	AATATT	1 cut(s)	277
SspMI	CTAG	2 cut(s)	36, 1236
StyD4I	CCNGG	1 cut(s)	1339
StyI	CCWWGG	1 cut(s)	35
TaaI	ACNGT	7 cut(s)	131, 224, 283, 370, 497, 571, 925
TaiI	ACGT	2 cut(s)	333, 878
TaqI	TCGA	6 cut(s)	882, 1039, 1104, 1468, 1505, 1513
TasI	AATT	6 cut(s)	408, 549, 620, 645, 1148, 1529
TauI	GCSGC	1 cut(s)	254
TfiI	GAWTC	5 cut(s)	815, 1079, 1355, 1477, 1510
Tru1I	TTAA	5 cut(s)	123, 407, 521, 648, 1499
Tru9I	TTAA	5 cut(s)	123, 407, 521, 648, 1499
TscAI	CASTG	3 cut(s)	229, 574, 1281
TseFI	GTSAC	3 cut(s)	331, 439, 1284
TseI	GCWGC	5 cut(s)	7, 265, 990, 1524, 1540
Tsp45I	GTSAC	3 cut(s)	331, 439, 1284
TspDTI	ATGAA	4 cut(s)	10, 70, 363, 1255
TspGWI	ACGGA	1 cut(s)	963
TspRI	CASTG	3 cut(s)	229, 574, 1281
Tth111I	GACNNNGTC	1 cut(s)	329
VneI	GTGCAC	1 cut(s)	1452
VpaK11BI	GGWCC	2 cut(s)	466, 1463
XapI	RAATTY	4 cut(s)	549, 620, 1148, 1529
XceI	RCATGY	2 cut(s)	595, 692
XmaJI	CCTAGG	1 cut(s)	35
XspI	CTAG	2 cut(s)	36, 1236
Zsp2I	ATGCAT	1 cut(s)	894

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.