Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0016306

Physical Location & Seq

Genomic Coordinates Reverse (-)

16410435 .. 16413697

Gene Structure 3263 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1191500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1053 bp

ATGGGTGATATTTCGTATTCCAATGGAACCAACAACGGGAATGGGAATGGGGGTGGGAATGTGAATGTGAATAGAAATGGAGTGAATGGCAAGGGCCATTCAGTGCTGAATGGATACAGGAAGAGCTGTTGGTATGAGGAAGAAATTGAAGAAAATTTGAGATGGAGCTTTGCTCTTAATAGTATATTGCATACAGGATCAACTCCATTTCAAGACATTGCATTGCTGGACACAAAGCCATTTGGAAAGGCTTTAGTAATTGATGGAAAGCTTCAAAGCGCTGAGACGGATGAATTTATCTACCACGAATGTCTTGTCCACCCACCACTTCTACATCATCCAAATCCAAAGAGCATCTTCATCATGGGAGGAGGTGAAGGCTCAACTGCAAGAGAACTTCTGAGGCACAGGACTGTGGAGAAGGTTGTCATGTGTGACATTGATGAGGAGGTGGTAGAGTTCTGCAAGTCATATTTGACTGTAAACAGTGAAGCTTTCTGTGATCCAAGACTGGAGCTCATCATCAATGATGCCAGGGCTGAGCTAGAACACAGGGAAGGGCAGTACGATGTGATCATTGGTGACCTAGCAGACCCAATAGAAGGAGGCCCATGTTATAAACTCTACACCAAATCCTTCTACGAGTTTACTGTCAAACCAAGACTCACCAAGGAAGGCATTTTTGTCACACAGGCAGGTCCTGCTGGAATTTTCAGCCACACAGAAGTATTTTCCTGCATTTACAATACTTTGAGGCAGGTCTTCAAATATGTTGTGCCTTATTCAGCTCATATTCCTTCTTATGCTGATACTTGGGGATGGATCCTGGCTTCAGATACCCCTTTTGAGCTTAGTGCCGATGAACTAGACCTCAGAATTAAACAAAGGATCACAGGGGAGAACAGATACCTAGATGGAAAAACATTTTCATCAGCTTCTACCTTGAGCAAAGCTGTTCGGAAATCGATGGTCAATGAGACTCATGTGTATACAGAAGGAACGGCAAGGTTCATATATGGCCATGGCCATGGGAGTGCCTACAAACTCAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0008150 GO:0009888 GO:0009914 GO:0009926 GO:0009987 GO:0010087 GO:0010089 GO:0010487 GO:0010817 GO:0016740 GO:0016765 GO:0016768 GO:0030154 GO:0032502 GO:0044424 GO:0044464 GO:0048759 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0060918 GO:0065007 GO:0065008 GO:1905177

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Spermine_synt_N Spermine_synth

Protein Analysis

351

Amino Acids

39.13

Weight (kDa)

5.64

Isoelectric Point (pI)

26.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spermine_synt_N	PF17284	44 - 95	9.8e-13	Spermidine synthase tetramerisation domain
Spermine_synth	PF01564	98 - 276	5.1e-45	Spermine/spermidine synthase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016306)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g15320 FvH4_4g15320
malus_domestica	MD13G1191500.v1.1 MD16G1191600.v1.1
prunus_persica	Prupe.1G011700_v2.0.a1
pyrus_communis	pycom13g16430 pycom16g16140
rosa_chinensis	RchiOBHm_Chr4g0416531
rosa_laevigata	RLG00000007976
rosa_roxburghii	Rroxscaffold_5G00360410
rosa_rugosa	Rorug04G0142400
rosa_samantha	Rh4AG205000 Rh4BG202700 Rh4DG202200
rosa_wichuraiana	Rw4G017350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	618
Acc36I	ACCTGC	2 cut(s)	686, 748
AccI	GTMKAC	1 cut(s)	989
AclWI	GGATC	5 cut(s)	205, 497, 817, 830, 896
AcoI	YGGCCR	2 cut(s)	1018, 1024
AcsI	RAATTY	3 cut(s)	154, 293, 708
AcuI	CTGAAG	1 cut(s)	816
AfaI	GTAC	1 cut(s)	566
AfeI	AGCGCT	1 cut(s)	280
AfiI	CCNNNNNNNGG	2 cut(s)	36, 602
AgsI	TTSAA	4 cut(s)	149, 212, 275, 766
AjnI	CCWGG	2 cut(s)	533, 825
Alw21I	GWGCWC	1 cut(s)	519
Alw26I	GTCTC	2 cut(s)	278, 971
AlwI	GGATC	5 cut(s)	205, 497, 817, 830, 896
AlwNI	CAGNNNCTG	1 cut(s)	701
Aor51HI	AGCGCT	1 cut(s)	280
AoxI	GGCC	4 cut(s)	94, 607, 1018, 1024
ApoI	RAATTY	3 cut(s)	154, 293, 708
ArsI	GACNNNNNNTTYG	2 cut(s)	300, 332
AspLEI	GCGC	1 cut(s)	281
AspS9I	GGNCC	3 cut(s)	94, 608, 698
AsuHPI	GGTGA	4 cut(s)	17, 386, 593, 658
AvaII	GGWCC	1 cut(s)	698
BalI	TGGCCA	2 cut(s)	1020, 1026
BamHI	GGATCC	1 cut(s)	822
BanII	GRGCYC	1 cut(s)	519
BbsI	GAAGAC	1 cut(s)	754
Bbv12I	GWGCWC	1 cut(s)	519
BccI	CCATC	5 cut(s)	156, 257, 813, 908, 961
BceAI	ACGGC	1 cut(s)	1017
BciT130I	CCWGG	2 cut(s)	535, 827
BciVI	GTATCC	1 cut(s)	107
BclI	TGATCA	1 cut(s)	573
BcoDI	GTCTC	2 cut(s)	278, 971
BfaI	CTAG	4 cut(s)	545, 587, 866, 911
BfoI	RGCGCY	1 cut(s)	282
BfuAI	ACCTGC	2 cut(s)	686, 748
BfuI	GTATCC	1 cut(s)	107
BlpI	GCTNAGC	1 cut(s)	540
Bme1390I	CCNGG	2 cut(s)	535, 827
Bme18I	GGWCC	1 cut(s)	698
BmgT120I	GGNCC	3 cut(s)	94, 608, 698
BmiI	GGNNCC	2 cut(s)	28, 824
BmrFI	CCNGG	2 cut(s)	535, 827
BmsI	GCATC	2 cut(s)	363, 520
BpiI	GAAGAC	1 cut(s)	754
BplI	GAGNNNNNCTC	2 cut(s)	157, 189
BpmI	CTGGAG	1 cut(s)	533
Bpu1102I	GCTNAGC	1 cut(s)	540
BpuEI	CTTGAG	1 cut(s)	964
Bsa29I	ATCGAT	1 cut(s)	965
BsaJI	CCNNGG	4 cut(s)	534, 669, 1021, 1027
Bsc4I	CCNNNNNNNGG	2 cut(s)	36, 602
Bse1I	ACTGG	1 cut(s)	516
Bse3DI	GCAATG	2 cut(s)	216, 221
BseBI	CCWGG	2 cut(s)	535, 827
BseCI	ATCGAT	1 cut(s)	965
BseDI	CCNNGG	4 cut(s)	534, 669, 1021, 1027
BseGI	GGATG	3 cut(s)	295, 337, 824
BseLI	CCNNNNNNNGG	2 cut(s)	36, 602
BseMI	GCAATG	2 cut(s)	216, 221
BseMII	CTCAG	4 cut(s)	273, 392, 531, 886
BseNI	ACTGG	1 cut(s)	516
BseRI	GAGGAG	2 cut(s)	384, 461
BshFI	GGCC	4 cut(s)	96, 609, 1020, 1026
BshVI	ATCGAT	1 cut(s)	965
BsiHKAI	GWGCWC	1 cut(s)	519
BslI	CCNNNNNNNGG	2 cut(s)	36, 602
BsmAI	GTCTC	2 cut(s)	278, 971
BsmBI	CGTCTC	1 cut(s)	278
BsnI	GGCC	4 cut(s)	96, 609, 1020, 1026
Bsp1286I	GDGCHC	1 cut(s)	519
Bsp143I	GATC	5 cut(s)	197, 502, 573, 822, 888
Bsp1720I	GCTNAGC	1 cut(s)	540
Bsp19I	CCATGG	2 cut(s)	1021, 1027
BspANI	GGCC	4 cut(s)	96, 609, 1020, 1026
BspCNI	CTCAG	4 cut(s)	274, 393, 532, 885
BspDI	ATCGAT	1 cut(s)	965
BspLI	GGNNCC	2 cut(s)	28, 824
BspMI	ACCTGC	2 cut(s)	686, 748
BspPI	GGATC	5 cut(s)	205, 497, 817, 830, 896
BspQI	GCTCTTC	1 cut(s)	116
BsrDI	GCAATG	2 cut(s)	216, 221
BsrI	ACTGG	1 cut(s)	516
BssECI	CCNNGG	4 cut(s)	534, 669, 1021, 1027
BssMI	GATC	5 cut(s)	197, 502, 573, 822, 888
BssNAI	GTATAC	1 cut(s)	990
BssT1I	CCWWGG	3 cut(s)	669, 1021, 1027
Bst1107I	GTATAC	1 cut(s)	990
Bst2UI	CCWGG	2 cut(s)	535, 827
Bst4CI	ACNGT	4 cut(s)	415, 481, 488, 652
Bst6I	CTCTTC	1 cut(s)	116
BstAPI	GCANNNNNTGC	1 cut(s)	701
BstDEI	CTNAG	5 cut(s)	282, 401, 540, 851, 872
BstDSI	CCRYGG	2 cut(s)	1021, 1027
BstEII	GGTNACC	1 cut(s)	581
BstF5I	GGATG	3 cut(s)	295, 337, 824
BstH2I	RGCGCY	1 cut(s)	282
BstHHI	GCGC	1 cut(s)	281
BstKTI	GATC	5 cut(s)	200, 505, 576, 825, 891
BstMAI	GTCTC	2 cut(s)	278, 971
BstMBI	GATC	5 cut(s)	197, 502, 573, 822, 888
BstMWI	GCNNNNNNNGC	1 cut(s)	701
BstNI	CCWGG	2 cut(s)	535, 827
BstPI	GGTNACC	1 cut(s)	581
BstSCI	CCNGG	2 cut(s)	533, 825
BstV2I	GAAGAC	1 cut(s)	754
BstX2I	RGATCY	1 cut(s)	822
BstXI	CCANNNNNNTGG	1 cut(s)	1028
BstYI	RGATCY	1 cut(s)	822
BstZ17I	GTATAC	1 cut(s)	990
Bsu15I	ATCGAT	1 cut(s)	965
BsuI	GTATCC	1 cut(s)	107
BsuRI	GGCC	4 cut(s)	96, 609, 1020, 1026
BsuTUI	ATCGAT	1 cut(s)	965
BtgI	CCRYGG	2 cut(s)	1021, 1027
BtsCI	GGATG	3 cut(s)	295, 337, 824
BtsIMutI	CAGTG	2 cut(s)	108, 493
BveI	ACCTGC	2 cut(s)	686, 748
CaiI	CAGNNNCTG	1 cut(s)	701
CfoI	GCGC	1 cut(s)	281
Cfr13I	GGNCC	3 cut(s)	94, 608, 698
ClaI	ATCGAT	1 cut(s)	965
Csp6I	GTAC	1 cut(s)	565
CviAII	CATG	6 cut(s)	364, 430, 612, 983, 1022, 1028
CviQI	GTAC	1 cut(s)	565
DdeI	CTNAG	5 cut(s)	282, 401, 540, 851, 872
DpnI	GATC	5 cut(s)	199, 504, 575, 824, 890
DpnII	GATC	5 cut(s)	197, 502, 573, 822, 888
EaeI	YGGCCR	2 cut(s)	1018, 1024
Eam1104I	CTCTTC	1 cut(s)	116
EarI	CTCTTC	1 cut(s)	116
Ecl136II	GAGCTC	1 cut(s)	517
Eco130I	CCWWGG	3 cut(s)	669, 1021, 1027
Eco24I	GRGCYC	1 cut(s)	519
Eco47I	GGWCC	1 cut(s)	698
Eco47III	AGCGCT	1 cut(s)	280
Eco53kI	GAGCTC	1 cut(s)	517
Eco57I	CTGAAG	1 cut(s)	816
Eco91I	GGTNACC	1 cut(s)	581
EcoICRI	GAGCTC	1 cut(s)	517
EcoO109I	RGGNCCY	1 cut(s)	698
EcoO65I	GGTNACC	1 cut(s)	581
EcoRII	CCWGG	2 cut(s)	533, 825
EcoT14I	CCWWGG	3 cut(s)	669, 1021, 1027
EcoT38I	GRGCYC	1 cut(s)	519
ErhI	CCWWGG	3 cut(s)	669, 1021, 1027
Esp3I	CGTCTC	1 cut(s)	278
FaeI	CATG	6 cut(s)	367, 433, 615, 986, 1025, 1031
FalI	AAGNNNNNCTT	2 cut(s)	341, 373
FatI	CATG	6 cut(s)	363, 429, 611, 982, 1021, 1027
FbaI	TGATCA	1 cut(s)	573
FblI	GTMKAC	1 cut(s)	989
FokI	GGATG	3 cut(s)	302, 324, 831
FriOI	GRGCYC	1 cut(s)	519
FspBI	CTAG	4 cut(s)	545, 587, 866, 911
GlaI	GCGC	1 cut(s)	280
GsuI	CTGGAG	1 cut(s)	533
HaeII	RGCGCY	1 cut(s)	282
HaeIII	GGCC	4 cut(s)	96, 609, 1020, 1026
HhaI	GCGC	1 cut(s)	281
Hin1II	CATG	6 cut(s)	367, 433, 615, 986, 1025, 1031
Hin6I	GCGC	1 cut(s)	279
HinP1I	GCGC	1 cut(s)	279
HindIII	AAGCTT	2 cut(s)	269, 492
HinfI	GANTC	2 cut(s)	663, 979
HphI	GGTGA	4 cut(s)	17, 386, 593, 658
Hpy166II	GTNNAC	4 cut(s)	319, 484, 648, 990
Hpy188I	TCNGA	4 cut(s)	402, 835, 875, 960
Hpy188III	TCNNGA	1 cut(s)	212
Hpy8I	GTNNAC	4 cut(s)	319, 484, 648, 990
HpyAV	CCTTC	8 cut(s)	371, 415, 551, 596, 646, 668, 807, 989
HpyCH4III	ACNGT	4 cut(s)	415, 481, 488, 652
HpyCH4V	TGCA	5 cut(s)	190, 221, 389, 465, 738
HpyF10VI	GCNNNNNNNGC	1 cut(s)	701
HpyF3I	CTNAG	5 cut(s)	282, 401, 540, 851, 872
Hsp92II	CATG	6 cut(s)	367, 433, 615, 986, 1025, 1031
HspAI	GCGC	1 cut(s)	279
Ksp22I	TGATCA	1 cut(s)	573
Kzo9I	GATC	5 cut(s)	197, 502, 573, 822, 888
LguI	GCTCTTC	1 cut(s)	116
LmnI	GCTCC	2 cut(s)	165, 514
LweI	GCATC	2 cut(s)	363, 520
MaeI	CTAG	4 cut(s)	545, 587, 866, 911
MaeIII	GTNAC	3 cut(s)	434, 581, 685
MalI	GATC	5 cut(s)	199, 504, 575, 824, 890
MboI	GATC	5 cut(s)	197, 502, 573, 822, 888
MboII	GAAGA	5 cut(s)	133, 152, 161, 349, 754
MflI	RGATCY	1 cut(s)	822
MhlI	GDGCHC	1 cut(s)	519
MlsI	TGGCCA	2 cut(s)	1020, 1026
MluCI	AATT	7 cut(s)	144, 154, 258, 293, 708, 876, 1048
MluNI	TGGCCA	2 cut(s)	1020, 1026
MlyI	GAGTC	2 cut(s)	657, 973
MnlI	CCTC	9 cut(s)	130, 362, 365, 396, 439, 442, 599, 747, 881
Mox20I	TGGCCA	2 cut(s)	1020, 1026
MscI	TGGCCA	2 cut(s)	1020, 1026
MseI	TTAA	3 cut(s)	177, 879, 1051
MslI	CAYNNNNRTG	2 cut(s)	1026, 1032
Msp20I	TGGCCA	2 cut(s)	1020, 1026
MspR9I	CCNGG	2 cut(s)	535, 827
MvaI	CCWGG	2 cut(s)	535, 827
MwoI	GCNNNNNNNGC	1 cut(s)	701
NcoI	CCATGG	2 cut(s)	1021, 1027
NdeII	GATC	5 cut(s)	197, 502, 573, 822, 888
NlaIII	CATG	6 cut(s)	367, 433, 615, 986, 1025, 1031
NlaIV	GGNNCC	2 cut(s)	28, 824
NmuCI	GTSAC	3 cut(s)	434, 581, 685
PciSI	GCTCTTC	1 cut(s)	116
PleI	GAGTC	2 cut(s)	657, 973
PpsI	GAGTC	2 cut(s)	657, 973
PpuMI	RGGWCCY	1 cut(s)	698
PsiI	TTATAA	1 cut(s)	618
Psp124BI	GAGCTC	1 cut(s)	519
Psp5II	RGGWCCY	1 cut(s)	698
Psp6I	CCWGG	2 cut(s)	533, 825
PspEI	GGTNACC	1 cut(s)	581
PspGI	CCWGG	2 cut(s)	533, 825
PspN4I	GGNNCC	2 cut(s)	28, 824
PspPI	GGNCC	3 cut(s)	94, 608, 698
PspPPI	RGGWCCY	1 cut(s)	698
PstNI	CAGNNNCTG	1 cut(s)	701
PsuI	RGATCY	1 cut(s)	822
RsaI	GTAC	1 cut(s)	566
RsaNI	GTAC	1 cut(s)	565
RseI	CAYNNNNRTG	2 cut(s)	1026, 1032
SacI	GAGCTC	1 cut(s)	519
SapI	GCTCTTC	1 cut(s)	116
SaqAI	TTAA	3 cut(s)	177, 879, 1051
Sau3AI	GATC	5 cut(s)	197, 502, 573, 822, 888
Sau96I	GGNCC	3 cut(s)	94, 608, 698
SchI	GAGTC	2 cut(s)	657, 973
ScrFI	CCNGG	2 cut(s)	535, 827
SduI	GDGCHC	1 cut(s)	519
SfaNI	GCATC	2 cut(s)	363, 520
SinI	GGWCC	1 cut(s)	698
SmiMI	CAYNNNNRTG	2 cut(s)	1026, 1032
SmlI	CTYRAG	1 cut(s)	943
SmoI	CTYRAG	1 cut(s)	943
Sse9I	AATT	7 cut(s)	144, 154, 258, 293, 708, 876, 1048
SspMI	CTAG	4 cut(s)	545, 587, 866, 911
SstI	GAGCTC	1 cut(s)	519
StyD4I	CCNGG	2 cut(s)	533, 825
StyI	CCWWGG	3 cut(s)	669, 1021, 1027
TaaI	ACNGT	4 cut(s)	415, 481, 488, 652
TaqI	TCGA	1 cut(s)	965
TasI	AATT	7 cut(s)	144, 154, 258, 293, 708, 876, 1048
Tru1I	TTAA	3 cut(s)	177, 879, 1051
Tru9I	TTAA	3 cut(s)	177, 879, 1051
TscAI	CASTG	2 cut(s)	108, 493
TseFI	GTSAC	3 cut(s)	434, 581, 685
Tsp45I	GTSAC	3 cut(s)	434, 581, 685
TspDTI	ATGAA	5 cut(s)	306, 349, 876, 918, 1000
TspGWI	ACGGA	1 cut(s)	302
TspRI	CASTG	2 cut(s)	108, 493
VpaK11BI	GGWCC	1 cut(s)	698
XapI	RAATTY	3 cut(s)	154, 293, 708
XmiI	GTMKAC	1 cut(s)	989
XspI	CTAG	4 cut(s)	545, 587, 866, 911

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.