Rh4DG202200

Thermospermine synthase ACAULIS5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0016306
Physical Location & Seq
Forward (+)
39305496 .. 39308564
3069 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG202200.1

Sequence Viewer

Length: 690 bp
ATGGGAGGAGGCGAAGGCTCCACTGCCAGAGAACTTCTGAGACACAAGACTGTGGAGAAGGTTGTCATGTGTGACATTGATGAGGAGGTGGTGGAGTTCTGCAAATCATTTTTGGATGTAAACAGAGAAGCTTTCTGTGATGCAAGACTCAAGCTCATCATCAATGATGCCAGGGCTGAGCTAGAACACACAGAAAACCACTATGATGTGATCATTGGAGATCTTGCAGACCCAATAGAAGGAGGACCATGTTATAAACTCTACACCAAATCTTTCTATGAGTTAATTGTCAAACCTAGACTCACTAAGGATGGCATTTTTGTCACACAGGCTGGTCCTGCTGGAATATTCAGCCACACTGAAGTATTCTCTTGCATCTACAATACTTTAAGGCAGGTCTTCAAATATGTTGTGCCTTATTCAGCTCATATTCCTTCTTATGCTGATACTTGGGGATGGATCATGGCTTCAGATACCCCATTTGTCCTTAGTGCTGATGAACTAGACATGAGAACTAAACTGAGGATCAATGGGGAGAACAGATACCTAGATGGGAAAACCTTTTCATCAGCCTCTACCTTAAGCAAAGCCGTTCGTAAATCGACGGAAAATGAGACTCATGTATATACAGAAGGAACTGCAAGGTTCATATATGGTCATGGTCATAATAGTGCCCAAAAACACAATTAA

Protein Analysis

229

Amino Acids

25.83

Weight (kDa)

5.85

Isoelectric Point (pI)

25.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spermine_synth PF01564 2 - 155 1.2e-37 Spermine/spermidine synthase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016306)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g15320 FvH4_4g15320
malus_domestica MD13G1191500.v1.1 MD16G1191600.v1.1
prunus_persica Prupe.1G011700_v2.0.a1
pyrus_communis pycom13g16430 pycom16g16140
rosa_chinensis RchiOBHm_Chr4g0416531
rosa_laevigata RLG00000007976
rosa_roxburghii Rroxscaffold_5G00360410
rosa_rugosa Rorug04G0142400
rosa_samantha Rh4AG205000 Rh4BG202700 Rh4DG202200
rosa_wichuraiana Rw4G017350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 255
Acc36I ACCTGC 1 cut(s) 385
AclWI GGATC 2 cut(s) 467, 533
AcuI CTGAAG 2 cut(s) 381, 453
AfiI CCNNNNNNNGG 1 cut(s) 239
AflII CTTAAG 1 cut(s) 580
AgsI TTSAA 1 cut(s) 403
AjnI CCWGG 1 cut(s) 170
AluBI AGCT 4 cut(s) 131, 154, 181, 425
AluI AGCT 4 cut(s) 131, 154, 181, 425
Alw26I GTCTC 2 cut(s) 34, 608
AlwI GGATC 2 cut(s) 467, 533
AspS9I GGNCC 2 cut(s) 245, 335
AvaII GGWCC 2 cut(s) 245, 335
BaeGI GKGCMC 1 cut(s) 676
BbsI GAAGAC 1 cut(s) 391
BccI CCATC 3 cut(s) 305, 450, 545
BceAI ACGGC 1 cut(s) 575
BciT130I CCWGG 1 cut(s) 172
BclI TGATCA 1 cut(s) 210
BcoDI GTCTC 2 cut(s) 34, 608
BfaI CTAG 4 cut(s) 182, 297, 503, 548
BfrI CTTAAG 1 cut(s) 580
BfuAI ACCTGC 1 cut(s) 385
BglII AGATCT 1 cut(s) 220
BlpI GCTNAGC 1 cut(s) 177
Bme1390I CCNGG 1 cut(s) 172
Bme18I GGWCC 2 cut(s) 245, 335
BmgT120I GGNCC 2 cut(s) 245, 335
BmiI GGNNCC 1 cut(s) 19
BmrFI CCNGG 1 cut(s) 172
BmsI GCATC 3 cut(s) 130, 157, 384
BpiI GAAGAC 1 cut(s) 391
Bpu1102I GCTNAGC 1 cut(s) 177
BpuEI CTTGAG 1 cut(s) 134
BsaJI CCNNGG 1 cut(s) 171
Bsc4I CCNNNNNNNGG 1 cut(s) 239
BseBI CCWGG 1 cut(s) 172
BseDI CCNNGG 1 cut(s) 171
BseGI GGATG 3 cut(s) 121, 316, 461
BseLI CCNNNNNNNGG 1 cut(s) 239
BseMII CTCAG 3 cut(s) 29, 168, 512
BseRI GAGGAG 2 cut(s) 21, 98
BseSI GKGCMC 1 cut(s) 676
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 2 cut(s) 34, 608
Bsp1286I GDGCHC 1 cut(s) 676
Bsp143I GATC 4 cut(s) 210, 220, 459, 525
Bsp1720I GCTNAGC 1 cut(s) 177
BspCNI CTCAG 3 cut(s) 30, 169, 513
BspLI GGNNCC 1 cut(s) 19
BspMI ACCTGC 1 cut(s) 385
BspPI GGATC 2 cut(s) 467, 533
BspTI CTTAAG 1 cut(s) 580
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 4 cut(s) 210, 220, 459, 525
Bst2UI CCWGG 1 cut(s) 172
Bst4CI ACNGT 1 cut(s) 52
BstAFI CTTAAG 1 cut(s) 580
BstDEI CTNAG 5 cut(s) 38, 177, 306, 488, 521
BstF5I GGATG 3 cut(s) 121, 316, 461
BstKTI GATC 4 cut(s) 213, 223, 462, 528
BstMAI GTCTC 2 cut(s) 34, 608
BstMBI GATC 4 cut(s) 210, 220, 459, 525
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstNI CCWGG 1 cut(s) 172
BstSCI CCNGG 1 cut(s) 170
BstSLI GKGCMC 1 cut(s) 676
BstV2I GAAGAC 1 cut(s) 391
BstX2I RGATCY 1 cut(s) 220
BstYI RGATCY 1 cut(s) 220
BtsCI GGATG 3 cut(s) 121, 316, 461
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 2 cut(s) 21, 357
BveI ACCTGC 1 cut(s) 385
Cfr13I GGNCC 2 cut(s) 245, 335
CviAII CATG 6 cut(s) 67, 249, 463, 508, 620, 659
DdeI CTNAG 5 cut(s) 38, 177, 306, 488, 521
DpnI GATC 4 cut(s) 212, 222, 461, 527
DpnII GATC 4 cut(s) 210, 220, 459, 525
Eco47I GGWCC 2 cut(s) 245, 335
Eco57I CTGAAG 2 cut(s) 381, 453
EcoRII CCWGG 1 cut(s) 170
FaeI CATG 6 cut(s) 70, 252, 466, 511, 623, 662
FatI CATG 6 cut(s) 66, 248, 462, 507, 619, 658
FbaI TGATCA 1 cut(s) 210
FokI GGATG 3 cut(s) 128, 323, 468
FspBI CTAG 4 cut(s) 182, 297, 503, 548
Hin1II CATG 6 cut(s) 70, 252, 466, 511, 623, 662
HindIII AAGCTT 1 cut(s) 129
HinfI GANTC 3 cut(s) 147, 300, 616
Hpy166II GTNNAC 1 cut(s) 121
Hpy188I TCNGA 2 cut(s) 39, 472
Hpy8I GTNNAC 1 cut(s) 121
Hpy99I CGWCG 1 cut(s) 607
HpyAV CCTTC 5 cut(s) 8, 52, 233, 444, 626
HpyCH4III ACNGT 1 cut(s) 52
HpyCH4V TGCA 5 cut(s) 102, 143, 227, 375, 641
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpyF3I CTNAG 5 cut(s) 38, 177, 306, 488, 521
Hsp92II CATG 6 cut(s) 70, 252, 466, 511, 623, 662
Ksp22I TGATCA 1 cut(s) 210
Kzo9I GATC 4 cut(s) 210, 220, 459, 525
LmnI GCTCC 1 cut(s) 23
LpnPI CCDG 8 cut(s) 40, 157, 184, 314, 318, 327, 351, 380
LweI GCATC 3 cut(s) 130, 157, 384
MaeI CTAG 4 cut(s) 182, 297, 503, 548
MaeIII GTNAC 2 cut(s) 71, 322
MalI GATC 4 cut(s) 212, 222, 461, 527
MboI GATC 4 cut(s) 210, 220, 459, 525
MboII GAAGA 1 cut(s) 391
MflI RGATCY 1 cut(s) 220
MhlI GDGCHC 1 cut(s) 676
MluCI AATT 2 cut(s) 285, 685
MlyI GAGTC 3 cut(s) 141, 294, 610
MnlI CCTC 5 cut(s) 76, 79, 236, 516, 583
MseI TTAA 4 cut(s) 284, 389, 581, 688
MslI CAYNNNNRTG 2 cut(s) 204, 669
MspCI CTTAAG 1 cut(s) 580
MspR9I CCNGG 1 cut(s) 172
MvaI CCWGG 1 cut(s) 172
MwoI GCNNNNNNNGC 1 cut(s) 338
NdeII GATC 4 cut(s) 210, 220, 459, 525
NlaIII CATG 6 cut(s) 70, 252, 466, 511, 623, 662
NlaIV GGNNCC 1 cut(s) 19
NmuCI GTSAC 2 cut(s) 71, 322
PleI GAGTC 3 cut(s) 141, 294, 610
PpsI GAGTC 3 cut(s) 141, 294, 610
PsiI TTATAA 1 cut(s) 255
Psp6I CCWGG 1 cut(s) 170
PspGI CCWGG 1 cut(s) 170
PspN4I GGNNCC 1 cut(s) 19
PspPI GGNCC 2 cut(s) 245, 335
PsuI RGATCY 1 cut(s) 220
RseI CAYNNNNRTG 2 cut(s) 204, 669
SaqAI TTAA 4 cut(s) 284, 389, 581, 688
Sau3AI GATC 4 cut(s) 210, 220, 459, 525
Sau96I GGNCC 2 cut(s) 245, 335
SchI GAGTC 3 cut(s) 141, 294, 610
ScrFI CCNGG 1 cut(s) 172
SduI GDGCHC 1 cut(s) 676
SfaNI GCATC 3 cut(s) 130, 157, 384
SinI GGWCC 2 cut(s) 245, 335
SmiMI CAYNNNNRTG 2 cut(s) 204, 669
SmlI CTYRAG 2 cut(s) 149, 580
SmoI CTYRAG 2 cut(s) 149, 580
Sse9I AATT 2 cut(s) 285, 685
SspI AATATT 1 cut(s) 348
SspMI CTAG 4 cut(s) 182, 297, 503, 548
StyD4I CCNGG 1 cut(s) 170
TaaI ACNGT 1 cut(s) 52
TaqI TCGA 1 cut(s) 602
TasI AATT 2 cut(s) 285, 685
Tru1I TTAA 4 cut(s) 284, 389, 581, 688
Tru9I TTAA 4 cut(s) 284, 389, 581, 688
TscAI CASTG 2 cut(s) 28, 364
TseFI GTSAC 2 cut(s) 71, 322
Tsp45I GTSAC 2 cut(s) 71, 322
TspDTI ATGAA 3 cut(s) 513, 555, 637
TspGWI ACGGA 1 cut(s) 620
TspRI CASTG 2 cut(s) 28, 364
Vha464I CTTAAG 1 cut(s) 580
VpaK11BI GGWCC 2 cut(s) 245, 335
XspI CTAG 4 cut(s) 182, 297, 503, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.