Rh4AG205000

Thermospermine synthase ACAULIS5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0016306
Physical Location & Seq
Forward (+)
49181958 .. 49184766
2809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG205000.1

Sequence Viewer

Length: 645 bp
ATGCAAGACTCAAGCTCATCATCAATGATGCCAGGTTTTCTTTGTCAATATAGTACATACATATATGCTAACCTTGGATCCGGCCACTATCTAATGTTATTGTTACTAATTCTTGAGGTGTTTTGCAGGGCTGAGCTAGAACACACAGAAAACCACTATGATGTGATCATTGGAGATCTTGCAGACCCAATAGAAGGAGGACCATGTTATAAACTCTACACCAAATCTTTCTATGAGTTAATTGTCAAACCTAGACTCACTAAGGATGGCATTTTTGTCACACAGGCTGGTCCTGCTGGAATATTCAGCCACACTGAAGTATTCTCTTGCATCTACAATACTTTAAGGCAGGTCTTCAAATATGTTGTGCCTTATTCAGCTCATATTCCTTCTTATGCTGATACTTGGGGATGGATCATGGCTTCAGATACCCCATTTGTCCTTAGTGCTGATGAACTAGACATGAGAACTAAACTGAGGATCAATGGGGAGAACAGATACCTAGATGGGAAAACCTTTTCATCAGCCTCTACCTTAAGCAAAGCCGTTCGTAAATCGACGGAAAATGAGACTCATGTATATACAGAAGGAACTGCAAGGTTCATATATGGTCATGGTCATAATAGTGCCCAAAAACACAATTAA

Protein Analysis

214

Amino Acids

24.16

Weight (kDa)

6.12

Isoelectric Point (pI)

31.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spermine_synth PF01564 46 - 140 2.7e-14 Spermine/spermidine synthase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016306)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g15320 FvH4_4g15320
malus_domestica MD13G1191500.v1.1 MD16G1191600.v1.1
prunus_persica Prupe.1G011700_v2.0.a1
pyrus_communis pycom13g16430 pycom16g16140
rosa_chinensis RchiOBHm_Chr4g0416531
rosa_laevigata RLG00000007976
rosa_roxburghii Rroxscaffold_5G00360410
rosa_rugosa Rorug04G0142400
rosa_samantha Rh4AG205000 Rh4BG202700 Rh4DG202200
rosa_wichuraiana Rw4G017350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 210
Acc36I ACCTGC 1 cut(s) 340
AclWI GGATC 4 cut(s) 72, 85, 422, 488
AcoI YGGCCR 1 cut(s) 82
AcuI CTGAAG 2 cut(s) 336, 408
AfaI GTAC 1 cut(s) 55
AfiI CCNNNNNNNGG 1 cut(s) 194
AflII CTTAAG 1 cut(s) 535
AgsI TTSAA 1 cut(s) 358
AjnI CCWGG 1 cut(s) 31
AluBI AGCT 3 cut(s) 15, 136, 380
AluI AGCT 3 cut(s) 15, 136, 380
Alw26I GTCTC 1 cut(s) 563
AlwI GGATC 4 cut(s) 72, 85, 422, 488
AoxI GGCC 1 cut(s) 82
AspS9I GGNCC 2 cut(s) 200, 290
AvaII GGWCC 2 cut(s) 200, 290
BaeGI GKGCMC 1 cut(s) 631
BamHI GGATCC 1 cut(s) 77
BbsI GAAGAC 1 cut(s) 346
BccI CCATC 3 cut(s) 260, 405, 500
BceAI ACGGC 1 cut(s) 530
BciT130I CCWGG 1 cut(s) 33
BclI TGATCA 1 cut(s) 165
BcoDI GTCTC 1 cut(s) 563
BfaI CTAG 4 cut(s) 137, 252, 458, 503
BfrI CTTAAG 1 cut(s) 535
BfuAI ACCTGC 1 cut(s) 340
BglII AGATCT 1 cut(s) 175
BlpI GCTNAGC 1 cut(s) 132
Bme1390I CCNGG 1 cut(s) 33
Bme18I GGWCC 2 cut(s) 200, 290
BmgT120I GGNCC 2 cut(s) 200, 290
BmiI GGNNCC 1 cut(s) 79
BmrFI CCNGG 1 cut(s) 33
BmsI GCATC 2 cut(s) 18, 339
BpiI GAAGAC 1 cut(s) 346
Bpu1102I GCTNAGC 1 cut(s) 132
BpuEI CTTGAG 1 cut(s) 134
BsaJI CCNNGG 1 cut(s) 73
Bsc4I CCNNNNNNNGG 1 cut(s) 194
BseBI CCWGG 1 cut(s) 33
BseDI CCNNGG 1 cut(s) 73
BseGI GGATG 2 cut(s) 271, 416
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMII CTCAG 2 cut(s) 123, 467
BseSI GKGCMC 1 cut(s) 631
BshFI GGCC 1 cut(s) 84
BsiSI CCGG 1 cut(s) 81
BslI CCNNNNNNNGG 1 cut(s) 194
BsmAI GTCTC 1 cut(s) 563
BsnI GGCC 1 cut(s) 84
Bsp1286I GDGCHC 1 cut(s) 631
Bsp143I GATC 5 cut(s) 77, 165, 175, 414, 480
Bsp1720I GCTNAGC 1 cut(s) 132
BspANI GGCC 1 cut(s) 84
BspCNI CTCAG 2 cut(s) 124, 468
BspLI GGNNCC 1 cut(s) 79
BspMI ACCTGC 1 cut(s) 340
BspPI GGATC 4 cut(s) 72, 85, 422, 488
BspTI CTTAAG 1 cut(s) 535
BssECI CCNNGG 1 cut(s) 73
BssMI GATC 5 cut(s) 77, 165, 175, 414, 480
BssT1I CCWWGG 1 cut(s) 73
Bst2UI CCWGG 1 cut(s) 33
BstAFI CTTAAG 1 cut(s) 535
BstDEI CTNAG 4 cut(s) 132, 261, 443, 476
BstF5I GGATG 2 cut(s) 271, 416
BstKTI GATC 5 cut(s) 80, 168, 178, 417, 483
BstMAI GTCTC 1 cut(s) 563
BstMBI GATC 5 cut(s) 77, 165, 175, 414, 480
BstMWI GCNNNNNNNGC 1 cut(s) 293
BstNI CCWGG 1 cut(s) 33
BstSCI CCNGG 1 cut(s) 31
BstSLI GKGCMC 1 cut(s) 631
BstV2I GAAGAC 1 cut(s) 346
BstX2I RGATCY 2 cut(s) 77, 175
BstYI RGATCY 2 cut(s) 77, 175
BsuRI GGCC 1 cut(s) 84
BtsCI GGATG 2 cut(s) 271, 416
BtsIMutI CAGTG 1 cut(s) 312
BveI ACCTGC 1 cut(s) 340
Cfr13I GGNCC 2 cut(s) 200, 290
Csp6I GTAC 1 cut(s) 54
CviAII CATG 5 cut(s) 204, 418, 463, 575, 614
CviQI GTAC 1 cut(s) 54
DdeI CTNAG 4 cut(s) 132, 261, 443, 476
DpnI GATC 5 cut(s) 79, 167, 177, 416, 482
DpnII GATC 5 cut(s) 77, 165, 175, 414, 480
EaeI YGGCCR 1 cut(s) 82
Eco130I CCWWGG 1 cut(s) 73
Eco47I GGWCC 2 cut(s) 200, 290
Eco57I CTGAAG 2 cut(s) 336, 408
EcoRII CCWGG 1 cut(s) 31
EcoT14I CCWWGG 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 73
FaeI CATG 5 cut(s) 207, 421, 466, 578, 617
FatI CATG 5 cut(s) 203, 417, 462, 574, 613
FbaI TGATCA 1 cut(s) 165
FokI GGATG 2 cut(s) 278, 423
FspBI CTAG 4 cut(s) 137, 252, 458, 503
HaeIII GGCC 1 cut(s) 84
HapII CCGG 1 cut(s) 81
Hin1II CATG 5 cut(s) 207, 421, 466, 578, 617
HinfI GANTC 3 cut(s) 8, 255, 571
HpaII CCGG 1 cut(s) 81
Hpy188I TCNGA 1 cut(s) 427
Hpy188III TCNNGA 1 cut(s) 113
Hpy99I CGWCG 1 cut(s) 562
HpyAV CCTTC 3 cut(s) 188, 399, 581
HpyCH4V TGCA 5 cut(s) 4, 126, 182, 330, 596
HpyF10VI GCNNNNNNNGC 1 cut(s) 293
HpyF3I CTNAG 4 cut(s) 132, 261, 443, 476
Hsp92II CATG 5 cut(s) 207, 421, 466, 578, 617
Ksp22I TGATCA 1 cut(s) 165
Kzo9I GATC 5 cut(s) 77, 165, 175, 414, 480
LpnPI CCDG 9 cut(s) 18, 45, 94, 112, 269, 273, 282, 306, 335
LweI GCATC 2 cut(s) 18, 339
MaeI CTAG 4 cut(s) 137, 252, 458, 503
MaeIII GTNAC 2 cut(s) 102, 277
MalI GATC 5 cut(s) 79, 167, 177, 416, 482
MboI GATC 5 cut(s) 77, 165, 175, 414, 480
MboII GAAGA 1 cut(s) 346
MflI RGATCY 2 cut(s) 77, 175
MhlI GDGCHC 1 cut(s) 631
MluCI AATT 3 cut(s) 108, 240, 640
MlyI GAGTC 3 cut(s) 2, 249, 565
MnlI CCTC 4 cut(s) 109, 191, 471, 538
MseI TTAA 4 cut(s) 239, 344, 536, 643
MslI CAYNNNNRTG 2 cut(s) 159, 624
MspCI CTTAAG 1 cut(s) 535
MspI CCGG 1 cut(s) 81
MspR9I CCNGG 1 cut(s) 33
MvaI CCWGG 1 cut(s) 33
MwoI GCNNNNNNNGC 1 cut(s) 293
NdeII GATC 5 cut(s) 77, 165, 175, 414, 480
NlaIII CATG 5 cut(s) 207, 421, 466, 578, 617
NlaIV GGNNCC 1 cut(s) 79
NmuCI GTSAC 1 cut(s) 277
PleI GAGTC 3 cut(s) 2, 249, 565
PpsI GAGTC 3 cut(s) 2, 249, 565
PsiI TTATAA 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 31
PspGI CCWGG 1 cut(s) 31
PspN4I GGNNCC 1 cut(s) 79
PspPI GGNCC 2 cut(s) 200, 290
PsuI RGATCY 2 cut(s) 77, 175
RsaI GTAC 1 cut(s) 55
RsaNI GTAC 1 cut(s) 54
RseI CAYNNNNRTG 2 cut(s) 159, 624
SaqAI TTAA 4 cut(s) 239, 344, 536, 643
Sau3AI GATC 5 cut(s) 77, 165, 175, 414, 480
Sau96I GGNCC 2 cut(s) 200, 290
SchI GAGTC 3 cut(s) 2, 249, 565
ScrFI CCNGG 1 cut(s) 33
SduI GDGCHC 1 cut(s) 631
SfaNI GCATC 2 cut(s) 18, 339
SinI GGWCC 2 cut(s) 200, 290
SmiMI CAYNNNNRTG 2 cut(s) 159, 624
SmlI CTYRAG 3 cut(s) 10, 113, 535
SmoI CTYRAG 3 cut(s) 10, 113, 535
Sse9I AATT 3 cut(s) 108, 240, 640
SspI AATATT 1 cut(s) 303
SspMI CTAG 4 cut(s) 137, 252, 458, 503
StyD4I CCNGG 1 cut(s) 31
StyI CCWWGG 1 cut(s) 73
TaqI TCGA 1 cut(s) 557
TasI AATT 3 cut(s) 108, 240, 640
TatI WGTACW 1 cut(s) 53
Tru1I TTAA 4 cut(s) 239, 344, 536, 643
Tru9I TTAA 4 cut(s) 239, 344, 536, 643
TscAI CASTG 1 cut(s) 319
TseFI GTSAC 1 cut(s) 277
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 3 cut(s) 468, 510, 592
TspGWI ACGGA 1 cut(s) 575
TspRI CASTG 1 cut(s) 319
Vha464I CTTAAG 1 cut(s) 535
VpaK11BI GGWCC 2 cut(s) 200, 290
XspI CTAG 4 cut(s) 137, 252, 458, 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.