Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0016306

Physical Location & Seq

Genomic Coordinates Reverse (-)

16928726 .. 16932263

Gene Structure 3538 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1191600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1035 bp

ATGGGCGATATTCCTGATTCCAATGGAACCAACAACGGGAATGGGAATGCGAATGGAAATGGAGTGAATGGCAAGGGATATTCAGTGCTGAATGGATACAGGAAGAGTTGTTGGTATGAGGAAGAAATTGAAGAAAATTTGAGATGGAGCTTTGCTCTTAATAGTATATTGCATACAGGAGCTACTCCATTCCAAGACATTGCATTGCTGGACACGAAGCCCTTTGGAAAGGCTTTAGTGATTGATGGAAAGCTTCAAAGTGCTGAGACGGATGAATTTATCTACCATGAATGTCTTGTGCACCCGCCACTTCTTCATCATCCCAATCCAAAGAGCATCTTCATCATGGGAGGAGGTGAAGGCTCCACTGCAAGAGAGCTTCTGAGACACAGGACTGTGGAGAAGGTTCTCATGTGTGACATTGATGAGGAGGTGGTAGGGTTCTGCAAGTCATATTTGATTGTAAACAGTGAAGCATTCTGTGATCCAAGATTGGAGCTCATCATCAATGATGCCAGGGCTGAGCTAGAACACAGAGAAGAGCAGTATGATGTGATCATTGGTGACCTTGCAGACCCAATAGAAGGAGGTCCATGTTATAAACTCTACACCAAATCCTTCTATGAATTAATTGTCAAGCCTAGACTCAGCAAGGAAGGCATTTTTGTCACACAGGCAGGTCCTGCTGGAATTTTCAGCCATACAGAAGTTTTCTCCTGCATTTACAATACTTTGAGGCAGGTCTTCAGATATGTTGTGCCTTATTCAGCTCATATTCCTTCTTATGCCGATATATGGGGATGGATCATGGCTTCAGACACCCCTATTGAGCTGAGTGCTGATGAATTAGACCTCAGAATTAAACAGAGGATCACAGGGGATAATAGATACCTAGATGGAAAAACATTTTCATCAGCCTCTACCTTGAGCAAAGCTGTTCGGAAATCGATGGTCAATGAGACTCATGTGTATACAGAAGGAACGGCAAGGTTCATATATGGCCATGGCCATGGCAGTGCCTACAAACTCGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0008150 GO:0009888 GO:0009914 GO:0009926 GO:0009987 GO:0010087 GO:0010089 GO:0010487 GO:0010817 GO:0016740 GO:0016765 GO:0016768 GO:0030154 GO:0032502 GO:0044424 GO:0044464 GO:0048759 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0060918 GO:0065007 GO:0065008 GO:1905177

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Spermine_synt_N Spermine_synth

Protein Analysis

345

Amino Acids

38.47

Weight (kDa)

5.34

Isoelectric Point (pI)

27.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spermine_synt_N	PF17284	38 - 89	1.2e-12	Spermidine synthase tetramerisation domain
Spermine_synth	PF01564	92 - 271	2.6e-44	Spermine/spermidine synthase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016306)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g15320 FvH4_4g15320
malus_domestica	MD13G1191500.v1.1 MD16G1191600.v1.1
prunus_persica	Prupe.1G011700_v2.0.a1
pyrus_communis	pycom13g16430 pycom16g16140
rosa_chinensis	RchiOBHm_Chr4g0416531
rosa_laevigata	RLG00000007976
rosa_roxburghii	Rroxscaffold_5G00360410
rosa_rugosa	Rorug04G0142400
rosa_samantha	Rh4AG205000 Rh4BG202700 Rh4DG202200
rosa_wichuraiana	Rw4G017350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	600
Acc36I	ACCTGC	2 cut(s)	668, 730
AccI	GTMKAC	1 cut(s)	971
AciI	CCGC	1 cut(s)	305
AclWI	GGATC	3 cut(s)	479, 812, 878
AcoI	YGGCCR	2 cut(s)	1000, 1006
AcsI	RAATTY	3 cut(s)	136, 275, 690
AcuI	CTGAAG	2 cut(s)	730, 798
AfiI	CCNNNNNNNGG	3 cut(s)	36, 584, 795
AgsI	TTSAA	2 cut(s)	131, 257
AjnI	CCWGG	1 cut(s)	515
AluBI	AGCT	9 cut(s)	150, 182, 253, 379, 499, 526, 770, 832, 935
AluI	AGCT	9 cut(s)	150, 182, 253, 379, 499, 526, 770, 832, 935
Alw21I	GWGCWC	2 cut(s)	303, 501
Alw26I	GTCTC	3 cut(s)	260, 379, 953
Alw44I	GTGCAC	1 cut(s)	299
AlwI	GGATC	3 cut(s)	479, 812, 878
AlwNI	CAGNNNCTG	1 cut(s)	683
AoxI	GGCC	2 cut(s)	1000, 1006
ApaLI	GTGCAC	1 cut(s)	299
ApoI	RAATTY	3 cut(s)	136, 275, 690
AseI	ATTAAT	1 cut(s)	629
AspS9I	GGNCC	2 cut(s)	590, 680
AsuHPI	GGTGA	2 cut(s)	368, 575
AvaII	GGWCC	2 cut(s)	590, 680
BaeGI	GKGCMC	1 cut(s)	303
BalI	TGGCCA	2 cut(s)	1002, 1008
BanII	GRGCYC	1 cut(s)	501
BbsI	GAAGAC	1 cut(s)	736
Bbv12I	GWGCWC	2 cut(s)	303, 501
BccI	CCATC	5 cut(s)	138, 239, 795, 890, 943
BceAI	ACGGC	1 cut(s)	999
BciT130I	CCWGG	1 cut(s)	517
BciVI	GTATCC	1 cut(s)	89
BclI	TGATCA	1 cut(s)	555
BcoDI	GTCTC	3 cut(s)	260, 379, 953
BfaI	CTAG	3 cut(s)	527, 642, 893
BfuAI	ACCTGC	2 cut(s)	668, 730
BfuI	GTATCC	1 cut(s)	89
BlpI	GCTNAGC	1 cut(s)	522
Bme1390I	CCNGG	1 cut(s)	517
Bme18I	GGWCC	2 cut(s)	590, 680
BmgT120I	GGNCC	2 cut(s)	590, 680
BmiI	GGNNCC	2 cut(s)	28, 364
BmrFI	CCNGG	1 cut(s)	517
BmsI	GCATC	2 cut(s)	345, 502
BpiI	GAAGAC	1 cut(s)	736
BplI	GAGNNNNNCTC	2 cut(s)	139, 171
Bpu1102I	GCTNAGC	1 cut(s)	522
BpuEI	CTTGAG	1 cut(s)	946
Bsa29I	ATCGAT	1 cut(s)	947
BsaJI	CCNNGG	3 cut(s)	516, 1003, 1009
Bsc4I	CCNNNNNNNGG	3 cut(s)	36, 584, 795
Bse3DI	GCAATG	2 cut(s)	198, 203
BseBI	CCWGG	1 cut(s)	517
BseCI	ATCGAT	1 cut(s)	947
BseDI	CCNNGG	3 cut(s)	516, 1003, 1009
BseGI	GGATG	3 cut(s)	277, 319, 806
BseLI	CCNNNNNNNGG	3 cut(s)	36, 584, 795
BseMI	GCAATG	2 cut(s)	198, 203
BseMII	CTCAG	6 cut(s)	255, 374, 513, 661, 824, 868
BseRI	GAGGAG	2 cut(s)	366, 443
BseSI	GKGCMC	1 cut(s)	303
BshFI	GGCC	2 cut(s)	1002, 1008
BshVI	ATCGAT	1 cut(s)	947
BsiHKAI	GWGCWC	2 cut(s)	303, 501
BslI	CCNNNNNNNGG	3 cut(s)	36, 584, 795
BsmAI	GTCTC	3 cut(s)	260, 379, 953
BsmBI	CGTCTC	1 cut(s)	260
BsmI	GAATGC	2 cut(s)	52, 476
BsnI	GGCC	2 cut(s)	1002, 1008
Bsp1286I	GDGCHC	2 cut(s)	303, 501
Bsp143I	GATC	4 cut(s)	484, 555, 804, 870
Bsp1720I	GCTNAGC	1 cut(s)	522
Bsp19I	CCATGG	2 cut(s)	1003, 1009
BspACI	CCGC	1 cut(s)	305
BspANI	GGCC	2 cut(s)	1002, 1008
BspCNI	CTCAG	6 cut(s)	256, 375, 514, 660, 825, 867
BspDI	ATCGAT	1 cut(s)	947
BspLI	GGNNCC	2 cut(s)	28, 364
BspMI	ACCTGC	2 cut(s)	668, 730
BspPI	GGATC	3 cut(s)	479, 812, 878
BspQI	GCTCTTC	1 cut(s)	534
BsrDI	GCAATG	2 cut(s)	198, 203
BssECI	CCNNGG	3 cut(s)	516, 1003, 1009
BssMI	GATC	4 cut(s)	484, 555, 804, 870
BssNAI	GTATAC	1 cut(s)	972
BssT1I	CCWWGG	2 cut(s)	1003, 1009
Bst1107I	GTATAC	1 cut(s)	972
Bst2UI	CCWGG	1 cut(s)	517
Bst4CI	ACNGT	2 cut(s)	397, 470
Bst6I	CTCTTC	2 cut(s)	98, 534
BstAPI	GCANNNNNTGC	1 cut(s)	683
BstDEI	CTNAG	6 cut(s)	264, 383, 522, 647, 833, 854
BstDSI	CCRYGG	2 cut(s)	1003, 1009
BstEII	GGTNACC	1 cut(s)	563
BstF5I	GGATG	3 cut(s)	277, 319, 806
BstKTI	GATC	4 cut(s)	487, 558, 807, 873
BstMAI	GTCTC	3 cut(s)	260, 379, 953
BstMBI	GATC	4 cut(s)	484, 555, 804, 870
BstMWI	GCNNNNNNNGC	2 cut(s)	657, 683
BstNI	CCWGG	1 cut(s)	517
BstPI	GGTNACC	1 cut(s)	563
BstSCI	CCNGG	1 cut(s)	515
BstSLI	GKGCMC	1 cut(s)	303
BstV2I	GAAGAC	1 cut(s)	736
BstXI	CCANNNNNNTGG	1 cut(s)	1010
BstZ17I	GTATAC	1 cut(s)	972
Bsu15I	ATCGAT	1 cut(s)	947
BsuI	GTATCC	1 cut(s)	89
BsuRI	GGCC	2 cut(s)	1002, 1008
BsuTUI	ATCGAT	1 cut(s)	947
BtgI	CCRYGG	2 cut(s)	1003, 1009
BtsCI	GGATG	3 cut(s)	277, 319, 806
BtsI	GCAGTG	2 cut(s)	366, 1021
BtsIMutI	CAGTG	4 cut(s)	90, 366, 475, 1021
BveI	ACCTGC	2 cut(s)	668, 730
CaiI	CAGNNNCTG	1 cut(s)	683
Cfr13I	GGNCC	2 cut(s)	590, 680
ClaI	ATCGAT	1 cut(s)	947
CviAII	CATG	8 cut(s)	287, 346, 412, 594, 808, 965, 1004, 1010
DdeI	CTNAG	6 cut(s)	264, 383, 522, 647, 833, 854
DpnI	GATC	4 cut(s)	486, 557, 806, 872
DpnII	GATC	4 cut(s)	484, 555, 804, 870
EaeI	YGGCCR	2 cut(s)	1000, 1006
Eam1104I	CTCTTC	2 cut(s)	98, 534
EarI	CTCTTC	2 cut(s)	98, 534
Ecl136II	GAGCTC	1 cut(s)	499
Eco130I	CCWWGG	2 cut(s)	1003, 1009
Eco24I	GRGCYC	1 cut(s)	501
Eco47I	GGWCC	2 cut(s)	590, 680
Eco53kI	GAGCTC	1 cut(s)	499
Eco57I	CTGAAG	2 cut(s)	730, 798
Eco91I	GGTNACC	1 cut(s)	563
EcoICRI	GAGCTC	1 cut(s)	499
EcoO109I	RGGNCCY	1 cut(s)	680
EcoO65I	GGTNACC	1 cut(s)	563
EcoRII	CCWGG	1 cut(s)	515
EcoT14I	CCWWGG	2 cut(s)	1003, 1009
EcoT38I	GRGCYC	1 cut(s)	501
ErhI	CCWWGG	2 cut(s)	1003, 1009
Esp3I	CGTCTC	1 cut(s)	260
FaeI	CATG	8 cut(s)	290, 349, 415, 597, 811, 968, 1007, 1013
FalI	AAGNNNNNCTT	2 cut(s)	323, 355
FatI	CATG	8 cut(s)	286, 345, 411, 593, 807, 964, 1003, 1009
FauI	CCCGC	1 cut(s)	312
FbaI	TGATCA	1 cut(s)	555
FblI	GTMKAC	1 cut(s)	971
FokI	GGATG	3 cut(s)	284, 306, 813
FriOI	GRGCYC	1 cut(s)	501
FspBI	CTAG	3 cut(s)	527, 642, 893
HaeIII	GGCC	2 cut(s)	1002, 1008
Hin1II	CATG	8 cut(s)	290, 349, 415, 597, 811, 968, 1007, 1013
HindIII	AAGCTT	1 cut(s)	251
HinfI	GANTC	3 cut(s)	17, 645, 961
HphI	GGTGA	2 cut(s)	368, 575
Hpy166II	GTNNAC	3 cut(s)	301, 466, 972
Hpy188I	TCNGA	5 cut(s)	384, 749, 817, 857, 942
Hpy188III	TCNNGA	1 cut(s)	14
Hpy8I	GTNNAC	3 cut(s)	301, 466, 972
HpyAV	CCTTC	7 cut(s)	353, 397, 578, 628, 650, 789, 971
HpyCH4III	ACNGT	2 cut(s)	397, 470
HpyCH4V	TGCA	7 cut(s)	172, 203, 301, 371, 447, 572, 720
HpyF10VI	GCNNNNNNNGC	2 cut(s)	657, 683
HpyF3I	CTNAG	6 cut(s)	264, 383, 522, 647, 833, 854
Hsp92II	CATG	8 cut(s)	290, 349, 415, 597, 811, 968, 1007, 1013
Ksp22I	TGATCA	1 cut(s)	555
Kzo9I	GATC	4 cut(s)	484, 555, 804, 870
LguI	GCTCTTC	1 cut(s)	534
LmnI	GCTCC	4 cut(s)	147, 179, 368, 496
LweI	GCATC	2 cut(s)	345, 502
MaeI	CTAG	3 cut(s)	527, 642, 893
MaeIII	GTNAC	3 cut(s)	416, 563, 667
MalI	GATC	4 cut(s)	486, 557, 806, 872
MboI	GATC	4 cut(s)	484, 555, 804, 870
MboII	GAAGA	7 cut(s)	115, 134, 143, 305, 331, 551, 736
MhlI	GDGCHC	2 cut(s)	303, 501
MlsI	TGGCCA	2 cut(s)	1002, 1008
MluCI	AATT	8 cut(s)	126, 136, 275, 626, 630, 690, 845, 858
MluNI	TGGCCA	2 cut(s)	1002, 1008
MlyI	GAGTC	2 cut(s)	639, 955
Mox20I	TGGCCA	2 cut(s)	1002, 1008
MscI	TGGCCA	2 cut(s)	1002, 1008
MseI	TTAA	4 cut(s)	159, 629, 861, 1033
MslI	CAYNNNNRTG	2 cut(s)	1008, 1014
Msp20I	TGGCCA	2 cut(s)	1002, 1008
MspR9I	CCNGG	1 cut(s)	517
Mva1269I	GAATGC	2 cut(s)	52, 476
MvaI	CCWGG	1 cut(s)	517
MwoI	GCNNNNNNNGC	2 cut(s)	657, 683
NcoI	CCATGG	2 cut(s)	1003, 1009
NdeII	GATC	4 cut(s)	484, 555, 804, 870
NlaIII	CATG	8 cut(s)	290, 349, 415, 597, 811, 968, 1007, 1013
NlaIV	GGNNCC	2 cut(s)	28, 364
NmuCI	GTSAC	3 cut(s)	416, 563, 667
PciSI	GCTCTTC	1 cut(s)	534
PctI	GAATGC	2 cut(s)	52, 476
PfeI	GAWTC	1 cut(s)	17
PleI	GAGTC	2 cut(s)	639, 955
PpsI	GAGTC	2 cut(s)	639, 955
PpuMI	RGGWCCY	1 cut(s)	680
PshBI	ATTAAT	1 cut(s)	629
PsiI	TTATAA	1 cut(s)	600
Psp124BI	GAGCTC	1 cut(s)	501
Psp5II	RGGWCCY	1 cut(s)	680
Psp6I	CCWGG	1 cut(s)	515
PspEI	GGTNACC	1 cut(s)	563
PspGI	CCWGG	1 cut(s)	515
PspN4I	GGNNCC	2 cut(s)	28, 364
PspPI	GGNCC	2 cut(s)	590, 680
PspPPI	RGGWCCY	1 cut(s)	680
PstNI	CAGNNNCTG	1 cut(s)	683
RseI	CAYNNNNRTG	2 cut(s)	1008, 1014
SacI	GAGCTC	1 cut(s)	501
SapI	GCTCTTC	1 cut(s)	534
SaqAI	TTAA	4 cut(s)	159, 629, 861, 1033
Sau3AI	GATC	4 cut(s)	484, 555, 804, 870
Sau96I	GGNCC	2 cut(s)	590, 680
SchI	GAGTC	2 cut(s)	639, 955
ScrFI	CCNGG	1 cut(s)	517
SduI	GDGCHC	2 cut(s)	303, 501
SfaNI	GCATC	2 cut(s)	345, 502
SinI	GGWCC	2 cut(s)	590, 680
SmiMI	CAYNNNNRTG	2 cut(s)	1008, 1014
SmlI	CTYRAG	1 cut(s)	925
SmoI	CTYRAG	1 cut(s)	925
Sse9I	AATT	8 cut(s)	126, 136, 275, 626, 630, 690, 845, 858
SsiI	CCGC	1 cut(s)	305
SspMI	CTAG	3 cut(s)	527, 642, 893
SstI	GAGCTC	1 cut(s)	501
StyD4I	CCNGG	1 cut(s)	515
StyI	CCWWGG	2 cut(s)	1003, 1009
TaaI	ACNGT	2 cut(s)	397, 470
TaqI	TCGA	2 cut(s)	947, 1029
TasI	AATT	8 cut(s)	126, 136, 275, 626, 630, 690, 845, 858
TfiI	GAWTC	1 cut(s)	17
Tru1I	TTAA	4 cut(s)	159, 629, 861, 1033
Tru9I	TTAA	4 cut(s)	159, 629, 861, 1033
TscAI	CASTG	4 cut(s)	90, 373, 475, 1021
TseFI	GTSAC	3 cut(s)	416, 563, 667
Tsp45I	GTSAC	3 cut(s)	416, 563, 667
TspDTI	ATGAA	8 cut(s)	288, 303, 305, 331, 639, 858, 900, 982
TspGWI	ACGGA	1 cut(s)	284
TspRI	CASTG	4 cut(s)	90, 373, 475, 1021
VneI	GTGCAC	1 cut(s)	299
VpaK11BI	GGWCC	2 cut(s)	590, 680
VspI	ATTAAT	1 cut(s)	629
XapI	RAATTY	3 cut(s)	136, 275, 690
XmiI	GTMKAC	1 cut(s)	971
XspI	CTAG	3 cut(s)	527, 642, 893

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.