MD13G1197900.v1.1

KIP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0006118
Physical Location & Seq
Forward (+)
17450989 .. 17459548
8560 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1197900.v1.1.491

Sequence Viewer

Length: 5958 bp
ATGGCAACCATGTTACATTCTGAGTCCAGACGATTATATTCTTGGTGGTGGGATAGTCACATTCCAAAGAACTCAAAATGGCTTCAGGAGAATCTTACAGACATGGATGCCAAAGTCAAAGCAATGATCAAGCTCATTGAAGAAGATGCAGATTCTTTTGCAAGGAGGGCAGAAATGTACTACAAGAAACGCCCGGAGCTCATGAAACTGGTGGAGGAGTTCTATCGAGCTTATCGTGCATTAGCAGAGAGGTACGATCATGCAACTGTGGAGCTGCGCCAGGCTCATCGAACCATGGCAGAAGCATTTCCCAACCAAGTACCTTATGTATTGGCTGATGAATCGTCCTCAGCCTCTTCTGGGCCAGACGTCGAACCTCATACACCAGAAATGCCACATCCAGTTTGTGCCTTATTTGACCCTGATGACTTGCATAAGGATGCATTGGGCCTCTTGTCAATCAATTCACAAGCTTTGAAAGGGAATGGAGGAAACTCAGCAGACTCCGACCCTGGAATAAGCAGAAGAGGTGGTCTAAAGCAGTTCATTGAGATGTTCACTCCAGGAGAAGTGCCAAATAGTTCAAAGGTTGCAGAGGGAATGATGGGACAAGGATTAAATTTTCATGAAGCAGAAGATTTCAAACAAAAGTTCCAAAATGGGTTTTCTCAGTTAACAAGTGAGAACCAGAGTCTCAAGACCCAGGTTCTTTCTCAATCTGAACGTGCTGCAAAAGCAGAAACTGAAGTTCAATCCTTAAAGAAAGCTCTAGATGAGATACAATTTGAAAAAGACGCAGTCCTTCTTCAGTACGAGCAGAGTTTGGAGAAGTTGTCTAAACTGGGGAGAGAACTAGATGATGCACAAAAGGCTATTGGAGGGCTTGATGAACGAGCAAGCAAAGCTGATATTGAAACTAAAATATTGAAGGAAGCCCTGGTGGAATTAGAAGCGGAGAGGGATGCTGGTCTTCTGCAGTACAATCGATGTTTGAAAAGGATATCTAGCCTGGAGAGCAAGTTATCTTTTTCCCAAAGGGATGCAAAAGGACTTAATGAGCTGGCTGTTAAAGCAGAAACTGAAGCTCAAAGTCTCAAGCAAGAACTTTCTAAACTAGAGGCTGAAAAGGAAGATTGTTTACTTCAGTACAAGCAATCTCTTGAGAAGATATCTGCTTTGGAGACGAAAATCTCCGTCTCTGAGGAAAATGTCAGAATGCTTAATGAACAAATTGAAAGAGCTCAAGGTGAAGTCAAATATTTGAAGGAATCTCTGGCTACACTGGAGGAAGGGAAAGAAGCTGCAGCTCTTCTATACAAACAGTGCATGGATACAATAGCTAAAATGGAAAGCGAAATTTCTAAGGCTCAAGCAGATGCTAAACGACTAAATAGCGAAGTTTTGACAGGCTCTGCAAAATTAAAGAGTGCCGAAGAACAATGTGTACTCTTGGAGAGATCAAATCACTCTCTGCGGTTAGAGGCGGACAGTCTGCTGAAGAAGATTACAACTAAGGATCAAGAACTTTCAGAGAAGGATGACCAGATGGAGAAACTTCACATTTTGATGGAAGAGGAGCACTTACAGTTTGTGCAAGCGGAAGCGACTCTTCAAGCTCTACAGAAGTTGCATTCTCAATCCCAGGAGGAACAGAAAGCTTTAGCACTGGAGTTCAAAAATGGGCTTCAAATGTTAAAGGACTTGGAGATACGCGAACAGGATATGAAGGATGATGTCCAACGGGCAAAGGAGGAAAATAAGAGCCTAAGTGAATTGAATTTTTCTTGCACTGTCTCCATAAAGAATCTGCAAGATGAAATCTTCAACATCAAAGAGATGAAAGAAAAACTTGAACAGGAGGTTGCGGTAAAATCAGACAAAAGCAATGCGCTCCAGCAGCATATTTTACATTTGGAGGAGGAAATTAAGGGATTAAATAGAAGATATCAAGCTATGGTGAAGCAGGTGGAGTCGGCTGGTTTAAATCCTGAATGCTTTGAATCATCTGTGAAAGACTTGCAAAATGAAAAATCGAAGCTGGAAGATATATGCACAAGGGGGAAAGAAGAGAGAGAACTTCTTTATGAGAAGTTGAAGGATACGGGTAAACTTTCAAAGGAGAATGATTTTATGGGGAGCTCGCTGTTGGGGTTGAACGCTGAGTTGGAGGGTTTGAGAGAGAAGGTCAAAGAATTGCAAGCGTCTTGCCAGTTTCTCCAGGGAGAAAAATCCACTCTTGTGGCTGAGAAGGCTCTCCTGCTTTCACAGTTACAAGTTATCACTCAGAATATGCAGGCGCTTTTTGAGAGGAACACCTTGCTGGACAATTCCCTCTCTGTTGCAGACATTGAGCTTGAACGGTTTAGAGCACGATCAAACAGCTTAGAAGAGTTGTGCCAATCTCTCAATAATGAGAAGTCCAGTCTGCTTAATGAGAGAGGCACCCTGGTATTTCAGTTGAAAGATGTTGAGGAGAGACTGCGAAACCTGGAAAAGCGCTTTACAAAATTAGAGAAGAAATATTCTAACCTGGGGAAGGAGAAGGGCTCCACACTTAGTGCAGTAGAAGAACTATGGGGTTCCCTCCATGCAGAAAAACGAGAGCGTGCAAGTTATATACGGTCAAGTGAGGCCCGATTGGCAGGTTTGGAAAACAATTTCCACCTCATGCAGGAAGAAAGAAAGCTGGGAAAGAAAGAATTTGAAGAAGAACTAGATAAAGCTTTAAATGCCCAGATCGAGATCTTTATCCTTCAGAAGTTTATAGAAGATCTGGAAGAGAAGAATTTGTCATTATTCATTGAGTGTCAGAGACACGTTGAGAAATCCAAGTTTTCTAGTAAGCTGATCTCAGAGTTGGAGAGCGAAAATCTCGAACTACAGGTGGAAGAACAATTCTTGGTGGAAGAAATCGACAAGTTGAGGTTGGGCGTTCATCAAGTCCTCAGGGCTCTACAGATTGAACCAGATAGGCACGACGATAAGACTGAACCAGGGAAAGTAAATGTGCTGCACATTTTGAATACTATCAAGGACTTGAAAACTTCTTTGTTACGGAGCAAGGATGAGGAACAGCAGTTGCTTGTTGAGAAGTCAGTGCTCTTAACTGTACTTGGGCAGTTGAGATCAGAGGGTGCAGAGGTAGAGACGGAAAAACAATTCTTCGAGCAGGAGTATGAGATCGTGATAAACCATTGTTCTACGCTACAAAATGAGAAGCGTGACCTTCTAGAGATGACAAGGGAGTTGAGATTGGAAGTGACGGAGAAAGAGCAAAGGGAGGAAATATTAGAGGCTGAATTGTTAACTCTTCAACCTAAGCTGGCAAATTTGGAGGATGCTTATGTGGTTCTGCAGGAAGAAAATTCCAAGGTGCTTGAAGAGAAGAGATCTTTGCTTAAGGAACTTATGGACCTCAAAGAGGAAAAGCAAATGCTTGAAGAGGATAATAGTGTTAATTTCCACGAAGCACTAGCTTTCAGCACCCTCTCGTTGGTTTTAGAGAGCTTCACAATTGAGAAAGCTGCGGAACTAAAAGCACTTGCTAAAGATCTCAACAGTCAATTTGTAATTAATAACAACCTCAAAGAGGCTGTTGGAACATTGGGGGAGAATTTAGTGATGAAAGAAGTAGAAAACCTGCATCTGAATGAGACGGTCCAATTGTTGGACAAGGAACTGTGTGAAGCCAAGGACTTAAATGGAGAACTAAGCCGTCAAGTTTTAGTTGGAAACGATTCTCTGAAACAGAAAACCTTGGAGCTCTCGGAGGCAGAAGAGAAGCTTAAAAAGACGGAAGATTTGAATGTGGAATTGTGCAGAACAATTCAGGAACTGAAGATGGAAATTGAAGAATCAAATCTAGTGCGAGAAAATTGTGAGAAGGAGATTTTTGAACTATCTGAAAATAGCACAAATCAGAAAAAGGAAATCAATAGCCTTTGTGAGGCAAACGAAATTCTGAAGAACCAAATCTTGTGTAACGTAATTGAAAAAGAAGTAGAAAATCTACATCTGAATGAGACAGTTCAATTGTTGCACAAGGAACTGCATGAAGCCAAGGACTCGAAAGATCAGCTGAACCATCAAATTTTAGCTGGAAAGGATTCTCTGAAGCAGAAAACCATGGAGCTCTCAGAAGTAGAAGAGAAGCTTAGAAAGGCCGAGAACTTGAATGTGGAATTATGCAGAACTGTTCAGGAAATGAGGATGGAACATGAAGATTCAAAGCGACTGAGGGAGAATTGTGTGAAGCAGATTCTAGAACTATCCAATGATTACTCAAATCAGAAAGATGAAATCGAGAGCCTTCGTGAAGCAAATGAAACCCTGGCGAATGAAGTGGGTATCTTAAGTGAAGTAATTGAAGAACATAGAACGAGAGAGGAGTATTTGAGTTCCGAGCTACAAGAGAGAAGCAATGACTTTGAACTCTGGGAGGCTGAGGCTGCGACATTCTATTTTGATCTTCAAGTTTCTGCTCTCCGTGAAGCTTTTCTTGAAAATACGGTTCATGAGCTCACTGATGTTTGTGAGAGTCTTAAAGATGAAAGTGCTGCGAAATCTGTGGAGGTCGAACAAATGAAAGAAAGAGTTAGCTCTTTGGAAGGTGAAGTTGGAGGACTGATCGGCCAGTTGTCTGCATATGTTCCTGCCGTAGCCTCATTGAGAGAGAATGTAGCATCTCTTCAGCACAATGCCGTACTTAGATCGAAGCTTCTTGTAGCAAGAAATCAACAATACGAGGATGTGAAACCGCCAAATCATCTATATGAAAAGAACGGTCAAGATTCCACAGAAGATCAAAGCCCATTGGTACACGACGGAATTTCAGAATTGGCAGAAGTGCAAACTATGATCAAAGAAGTTGAAATGACGTTTGTGGAAGAGACGGAAAGGCTTGCGATGGAAGCCTTTGAAAAGGCAATGGTGGAAGAAATTGAAAGCCTTTCAACTCAGGAAAGTACAAAAAACAGCAACTCGTCTGTGGAGATTGAAGAGTTACAATCAAAAGGTGCCTTACTTCAAGAACAAGGCAGTAAATGTGAAGAGGTGAAACTTGGGAAGGAGTTCACTGGTGAAAATCGCAAGTTGCAGAAGACAAAATCTGCTAATGGGACTTTGATGAAAGACATTCCGCTTGATCATGTTTCTGATTTATCATCGTATGGAAGAAGCTGGAGAGGTACTGGTGGGGCTGATGATCAGATGCTTGAGTTATGGGAAACTGCCGAACAATACTGTCGCCAGGATCCGATTGCCAGTGAGAAAGAAAATCAGGCATCTGCACGCATGGAAGATGTTACCCCCAGCCATCGGTTTACAGATTCTGAGCAGATGATTCAGAATTTTTCTTCAGATGTTGAGGCTGAGAAGGAGTGGGGAATTGAGAAGCTGGAAGCGTCCTTTCGAAGGCCGCGTCATGAAGGCAAGAAGGAAAAAATCTTGGAGAGACTGGCTTCTGATGCCCAGAAACTGACAAGTCTGCAGACAATTGCTCGGGATTTGGATAAGAAGATGGAGACAAGCAAGAAGGGTAAAAGGGCCAACGGCATTGAATATGAAACAGTCAAAAGACACTTACTTGAAGTTGAGTCTGCTGTTGTGCAGCTAGTGGAAACTAATGACCAACTGAAAAAGAAGATTGAGGAGTCTCCGTCTTTGGACGAGCAGACTTCCATGGAGTTGGAGGAGGCTGGAAATGTACGCAGAGAGAGAGTAGTCGAAGAGGCAAGTAAAGGGTCAGAGAAGATCGGAAGGCTGCAGTTTGAGTTGCAGAACATTCACTACATTTTGCTCAAACTGGAGGATGAGAATAAAAAGAAAGGGAGGCACAGATTTTACGTGAGCAGAACGGGCGTTCTCCTGAGGGATTTTATTTACAGCGGCAGAAGCAGCGAGAGGCGCAGGAAGGGTTGTGTGTGTGGCTGCATGAGACCCTCCACCAAGGGAGATTGA

Protein Analysis

1986

Amino Acids

227.16

Weight (kDa)

4.99

Isoelectric Point (pI)

51.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 14 - 86 1.2e-36 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1954
AatII GACGTC 1 cut(s) 372
Acc36I ACCTGC 3 cut(s) 1954, 2633, 3648
AccB1I GGYRCC 2 cut(s) 2441, 5015
AccB7I CCANNNNNTGG 1 cut(s) 3667
AccII CGCG 2 cut(s) 1713, 5419
AclWI GGATC 3 cut(s) 1524, 5246, 5259
AcoI YGGCCR 1 cut(s) 4630
AcyI GRCGYC 1 cut(s) 369
AdeI CACNNNGTG 1 cut(s) 2558
AfeI AGCGCT 1 cut(s) 2498
AflII CTTAAG 2 cut(s) 3398, 4351
AflIII ACRYGT 1 cut(s) 2815
AjuI GAANNNNNNNTTGG 4 cut(s) 2147, 2179, 4599, 4631
AleI CACNNNNGTG 1 cut(s) 2237
Alw21I GWGCWC 9 cut(s) 201, 1243, 1581, 2141, 2371, 3102, 3765, 4134, 4521
AlwI GGATC 3 cut(s) 1524, 5246, 5259
AlwNI CAGNNNCTG 6 cut(s) 743, 1079, 1412, 3835, 5330, 5476
Ama87I CYCGRG 1 cut(s) 5499
Aor51HI AGCGCT 1 cut(s) 2498
AoxI GGCC 7 cut(s) 362, 448, 2631, 4161, 4630, 5414, 5544
ArsI GACNNNNNNTTYG 4 cut(s) 3784, 3816, 5404, 5436
AseI ATTAAT 1 cut(s) 3573
Asp700I GAANNNNTTC 5 cut(s) 306, 3736, 4105, 4494, 5069
AspLEI GCGC 5 cut(s) 279, 1891, 2299, 2499, 5907
AspS9I GGNCC 6 cut(s) 362, 448, 2632, 3412, 3658, 5544
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 5 cut(s) 1259, 1969, 4622, 5065, 5090
AsuII TTCGAA 1 cut(s) 5410
AvaI CYCGRG 1 cut(s) 5499
AvaII GGWCC 2 cut(s) 3412, 3658
AxyI CCTNAGG 2 cut(s) 2945, 5867
BamHI GGATCC 1 cut(s) 5251
BanI GGYRCC 2 cut(s) 2441, 5015
BanII GRGCYC 8 cut(s) 201, 1243, 2141, 2549, 2953, 3765, 4134, 4521
BarI GAAGNNNNNNTAC 4 cut(s) 1122, 1154, 4671, 4703
BbsI GAAGAC 2 cut(s) 962, 5105
Bbv12I GWGCWC 9 cut(s) 201, 1243, 1581, 2141, 2371, 3102, 3765, 4134, 4521
BbvCI CCTCAGC 2 cut(s) 349, 4443
BccI CCATC 9 cut(s) 598, 1540, 1561, 3835, 4092, 4204, 4900, 5322, 5512
BceAI ACGGC 4 cut(s) 3699, 4640, 4685, 5566
BcgI CGANNNNNNTGC 2 cut(s) 965, 999
BciVI GTATCC 2 cut(s) 1324, 2092
BclI TGATCA 4 cut(s) 126, 4857, 5143, 5203
BcnI CCSGG 1 cut(s) 194
BfmI CTRYAG 8 cut(s) 974, 1302, 1619, 2879, 2954, 3353, 5486, 5762
BfoI RGCGCY 2 cut(s) 2300, 2500
BfrI CTTAAG 2 cut(s) 3398, 4351
BfuAI ACCTGC 3 cut(s) 1954, 2633, 3648
BfuI GTATCC 2 cut(s) 1324, 2092
BglI GCCNNNNNGGC 1 cut(s) 2639
BglII AGATCT 4 cut(s) 2742, 2770, 3389, 3550
Bme18I GGWCC 2 cut(s) 3412, 3658
BmeT110I CYCGRG 1 cut(s) 5499
BmgT120I GGNCC 6 cut(s) 362, 448, 2632, 3412, 3658, 5544
BmiI GGNNCC 5 cut(s) 2443, 2548, 2581, 5017, 5253
BmrI ACTGGG 1 cut(s) 851
BmuI ACTGGG 1 cut(s) 851
BpiI GAAGAC 2 cut(s) 962, 5105
BplI GAGNNNNNCTC 2 cut(s) 2531, 2563
BpmI CTGGAG 8 cut(s) 546, 1031, 1304, 1688, 1877, 2201, 5200, 5825
Bpu10I CCTNAGC 3 cut(s) 349, 3318, 4443
Bpu14I TTCGAA 1 cut(s) 5410
BpuEI CTTGAG 6 cut(s) 680, 1079, 1181, 1227, 1353, 5234
BpuMI CCSGG 1 cut(s) 194
Bsa29I ATCGAT 1 cut(s) 985
BsaAI YACGTR 1 cut(s) 5845
BsaBI GATNNNNATC 2 cut(s) 2741, 2747
BsaHI GRCGYC 1 cut(s) 369
BsaI GGTCTC 1 cut(s) 5929
BsaXI ACNNNNNCTCC 6 cut(s) 818, 848, 1004, 1034, 5642, 5672
Bse21I CCTNAGG 2 cut(s) 2945, 5867
Bse3DI GCAATG 4 cut(s) 129, 1891, 4426, 4932
Bse8I GATNNNNATC 2 cut(s) 2741, 2747
BseCI ATCGAT 1 cut(s) 985
BseJI GATNNNNATC 2 cut(s) 2741, 2747
BseMI GCAATG 4 cut(s) 129, 1891, 4426, 4932
BseRI GAGGAG 7 cut(s) 230, 1589, 1931, 2486, 4400, 5663, 5705
BseYI CCCAGC 2 cut(s) 2686, 5309
BsgI GTGCAG 6 cut(s) 2580, 2996, 3156, 3838, 5271, 5627
Bsh1236I CGCG 2 cut(s) 1713, 5419
BshFI GGCC 7 cut(s) 364, 450, 2633, 4163, 4632, 5416, 5546
BshNI GGYRCC 2 cut(s) 2441, 5015
BshVI ATCGAT 1 cut(s) 985
BsiHKAI GWGCWC 9 cut(s) 201, 1243, 1581, 2141, 2371, 3102, 3765, 4134, 4521
BsiHKCI CYCGRG 1 cut(s) 5499
BsiSI CCGG 1 cut(s) 194
BslFI GGGAC 2 cut(s) 621, 5131
BsmBI CGTCTC 5 cut(s) 1175, 1201, 3140, 3647, 4883
BsmFI GGGAC 2 cut(s) 621, 5131
BsmI GAATGC 3 cut(s) 1221, 1630, 1997
BsnI GGCC 7 cut(s) 364, 450, 2633, 4163, 4632, 5416, 5546
Bso31I GGTCTC 1 cut(s) 5929
BsoBI CYCGRG 1 cut(s) 5499
Bsp119I TTCGAA 1 cut(s) 5410
Bsp19I CCATGG 3 cut(s) 294, 4125, 5679
BspANI GGCC 7 cut(s) 364, 450, 2633, 4163, 4632, 5416, 5546
BspDI ATCGAT 1 cut(s) 985
BspFNI CGCG 2 cut(s) 1713, 5419
BspHI TCATGA 4 cut(s) 201, 625, 4513, 5422
BspLI GGNNCC 5 cut(s) 2443, 2548, 2581, 5017, 5253
BspMAI CTGCAG 5 cut(s) 978, 1306, 3357, 5490, 5766
BspMI ACCTGC 3 cut(s) 1954, 2633, 3648
BspPI GGATC 3 cut(s) 1524, 5246, 5259
BspQI GCTCTTC 1 cut(s) 1314
BspT104I TTCGAA 1 cut(s) 5410
BspT107I GGYRCC 2 cut(s) 2441, 5015
BspTI CTTAAG 2 cut(s) 3398, 4351
BspTNI GGTCTC 1 cut(s) 5929
BsrDI GCAATG 4 cut(s) 129, 1891, 4426, 4932
BssNI GRCGYC 1 cut(s) 369
BssT1I CCWWGG 8 cut(s) 294, 3369, 3690, 3756, 4059, 4125, 5679, 5946
BstACI GRCGYC 1 cut(s) 369
BstAFI CTTAAG 2 cut(s) 3398, 4351
BstBAI YACGTR 1 cut(s) 5845
BstBI TTCGAA 1 cut(s) 5410
BstDSI CCRYGG 3 cut(s) 294, 4125, 5679
BstENI CCTNNNNNAGG 6 cut(s) 2534, 2669, 3419, 3587, 3944, 5410
BstFNI CGCG 2 cut(s) 1713, 5419
BstH2I RGCGCY 2 cut(s) 2300, 2500
BstHHI GCGC 5 cut(s) 279, 1891, 2299, 2499, 5907
BstSFI CTRYAG 8 cut(s) 974, 1302, 1619, 2879, 2954, 3353, 5486, 5762
BstUI CGCG 2 cut(s) 1713, 5419
BstV2I GAAGAC 2 cut(s) 962, 5105
BstX2I RGATCY 5 cut(s) 2742, 2770, 3389, 3550, 5251
BstXI CCANNNNNNTGG 2 cut(s) 2239, 5686
BstYI RGATCY 5 cut(s) 2742, 2770, 3389, 3550, 5251
Bsu15I ATCGAT 1 cut(s) 985
Bsu36I CCTNAGG 2 cut(s) 2945, 5867
BsuI GTATCC 2 cut(s) 1324, 2092
BsuRI GGCC 7 cut(s) 364, 450, 2633, 4163, 4632, 5416, 5546
BsuTUI ATCGAT 1 cut(s) 985
BtgI CCRYGG 3 cut(s) 294, 4125, 5679
BtgZI GCGATG 1 cut(s) 4919
BtsIMutI CAGTG 8 cut(s) 1280, 1328, 1664, 1788, 3102, 4521, 5073, 5269
BveI ACCTGC 3 cut(s) 1954, 2633, 3648
CaiI CAGNNNCTG 6 cut(s) 743, 1079, 1412, 3835, 5330, 5476
CciI TCATGA 4 cut(s) 201, 625, 4513, 5422
CfoI GCGC 5 cut(s) 279, 1891, 2299, 2499, 5907
Cfr13I GGNCC 6 cut(s) 362, 448, 2632, 3412, 3658, 5544
ClaI ATCGAT 1 cut(s) 985
CseI GACGC 4 cut(s) 803, 2190, 5391, 5408
DraI TTTAAA 2 cut(s) 1983, 2727
DraIII CACNNNGTG 1 cut(s) 2558
EaeI YGGCCR 1 cut(s) 4630
EciI GGCGGA 1 cut(s) 1499
Ecl136II GAGCTC 6 cut(s) 199, 1241, 2139, 3763, 4132, 4519
Eco130I CCWWGG 8 cut(s) 294, 3369, 3690, 3756, 4059, 4125, 5679, 5946
Eco24I GRGCYC 8 cut(s) 201, 1243, 2141, 2549, 2953, 3765, 4134, 4521
Eco31I GGTCTC 1 cut(s) 5929
Eco32I GATATC 3 cut(s) 1002, 1170, 1946
Eco47I GGWCC 2 cut(s) 3412, 3658
Eco47III AGCGCT 1 cut(s) 2498
Eco53kI GAGCTC 6 cut(s) 199, 1241, 2139, 3763, 4132, 4519
Eco81I CCTNAGG 2 cut(s) 2945, 5867
Eco88I CYCGRG 1 cut(s) 5499
EcoICRI GAGCTC 6 cut(s) 199, 1241, 2139, 3763, 4132, 4519
EcoNI CCTNNNNNAGG 6 cut(s) 2534, 2669, 3419, 3587, 3944, 5410
EcoRV GATATC 3 cut(s) 1002, 1170, 1946
EcoT14I CCWWGG 8 cut(s) 294, 3369, 3690, 3756, 4059, 4125, 5679, 5946
EcoT22I ATGCAT 1 cut(s) 445
EcoT38I GRGCYC 8 cut(s) 201, 1243, 2141, 2549, 2953, 3765, 4134, 4521
ErhI CCWWGG 8 cut(s) 294, 3369, 3690, 3756, 4059, 4125, 5679, 5946
Esp3I CGTCTC 5 cut(s) 1175, 1201, 3140, 3647, 4883
FaqI GGGAC 2 cut(s) 621, 5131
FauNDI CATATG 1 cut(s) 4645
FbaI TGATCA 4 cut(s) 126, 4857, 5143, 5203
FriOI GRGCYC 8 cut(s) 201, 1243, 2141, 2549, 2953, 3765, 4134, 4521
GlaI GCGC 5 cut(s) 278, 1890, 2298, 2498, 5906
GsaI CCCAGC 2 cut(s) 2690, 5313
GsuI CTGGAG 8 cut(s) 546, 1031, 1304, 1688, 1877, 2201, 5200, 5825
HaeII RGCGCY 2 cut(s) 2300, 2500
HaeIII GGCC 7 cut(s) 364, 450, 2633, 4163, 4632, 5416, 5546
HapII CCGG 1 cut(s) 194
HgaI GACGC 4 cut(s) 803, 2190, 5391, 5408
HhaI GCGC 5 cut(s) 279, 1891, 2299, 2499, 5907
Hin1I GRCGYC 1 cut(s) 369
Hin6I GCGC 5 cut(s) 277, 1889, 2297, 2497, 5905
HinP1I GCGC 5 cut(s) 277, 1889, 2297, 2497, 5905
HincII GTYRAC 2 cut(s) 675, 3306
HindII GTYRAC 2 cut(s) 675, 3306
HindIII AAGCTT 7 cut(s) 471, 1656, 2721, 3782, 4151, 4491, 4715
HpaI GTTAAC 2 cut(s) 675, 3306
HpaII CCGG 1 cut(s) 194
HphI GGTGA 5 cut(s) 1259, 1969, 4622, 5065, 5090
Hpy166II GTNNAC 9 cut(s) 558, 675, 1139, 1445, 2108, 3306, 4819, 5073, 5322
Hpy8I GTNNAC 9 cut(s) 558, 675, 1139, 1445, 2108, 3306, 4819, 5073, 5322
Hpy99I CGWCG 3 cut(s) 374, 2981, 4826
HpyCH4IV ACGT 6 cut(s) 369, 724, 2817, 3984, 4877, 5844
HpySE526I ACGT 6 cut(s) 369, 724, 2817, 3984, 4877, 5844
Hsp92I GRCGYC 1 cut(s) 369
HspAI GCGC 5 cut(s) 277, 1889, 2297, 2497, 5905
Ksp22I TGATCA 4 cut(s) 126, 4857, 5143, 5203
KspAI GTTAAC 2 cut(s) 675, 3306
LguI GCTCTTC 1 cut(s) 1314
LmnI GCTCC 9 cut(s) 196, 271, 1576, 1896, 2136, 2552, 3057, 3760, 4129
MaeII ACGT 6 cut(s) 369, 724, 2817, 3984, 4877, 5844
MaeIII GTNAC 9 cut(s) 12, 56, 2268, 3051, 3221, 3259, 3980, 5001, 5302
MfeI CAATTG 4 cut(s) 3513, 3662, 4031, 5493
MflI RGATCY 5 cut(s) 2742, 2770, 3389, 3550, 5251
MlyI GAGTC 9 cut(s) 32, 497, 700, 1600, 1979, 4058, 4546, 5603, 5660
MmeI TCCRAC 9 cut(s) 531, 1762, 2145, 2838, 3577, 3648, 3709, 4597, 5667
Mph1103I ATGCAT 1 cut(s) 445
MroXI GAANNNNTTC 5 cut(s) 306, 3736, 4105, 4494, 5069
MslI CAYNNNNRTG 7 cut(s) 438, 551, 1565, 2237, 3648, 4017, 4770
MspA1I CMGCKG 2 cut(s) 4078, 5886
MspCI CTTAAG 2 cut(s) 3398, 4351
MspI CCGG 1 cut(s) 194
MunI CAATTG 4 cut(s) 3513, 3662, 4031, 5493
Mva1269I GAATGC 3 cut(s) 1221, 1630, 1997
MvnI CGCG 2 cut(s) 1713, 5419
NciI CCSGG 1 cut(s) 194
NcoI CCATGG 3 cut(s) 294, 4125, 5679
NdeI CATATG 1 cut(s) 4645
NlaIV GGNNCC 5 cut(s) 2443, 2548, 2581, 5017, 5253
NmeAIII GCCGAG 1 cut(s) 4189
NmuCI GTSAC 3 cut(s) 56, 3221, 3259
NsiI ATGCAT 1 cut(s) 445
NspV TTCGAA 1 cut(s) 5410
OliI CACNNNNGTG 1 cut(s) 2237
PagI TCATGA 4 cut(s) 201, 625, 4513, 5422
PaqCI CACCTGC 1 cut(s) 1954
PciSI GCTCTTC 1 cut(s) 1314
PcsI WCGNNNNNNNCGW 2 cut(s) 232, 5416
PctI GAATGC 3 cut(s) 1221, 1630, 1997
PdmI GAANNNNTTC 5 cut(s) 306, 3736, 4105, 4494, 5069
PflFI GACNNNGTC 1 cut(s) 797
PflMI CCANNNNNTGG 1 cut(s) 3667
PfoI TCCNGGA 1 cut(s) 562
PleI GAGTC 9 cut(s) 31, 497, 699, 1600, 1978, 4058, 4545, 5602, 5659
PpsI GAGTC 9 cut(s) 31, 497, 699, 1600, 1978, 4058, 4545, 5602, 5659
Ppu21I YACGTR 1 cut(s) 5845
PshBI ATTAAT 1 cut(s) 3573
Psp124BI GAGCTC 6 cut(s) 201, 1243, 2141, 3765, 4134, 4521
PspFI CCCAGC 2 cut(s) 2686, 5309
PspN4I GGNNCC 5 cut(s) 2443, 2548, 2581, 5017, 5253
PspPI GGNCC 6 cut(s) 362, 448, 2632, 3412, 3658, 5544
PsrI GAACNNNNNNTAC 4 cut(s) 1428, 1460, 5771, 5803
PstI CTGCAG 5 cut(s) 978, 1306, 3357, 5490, 5766
PstNI CAGNNNCTG 6 cut(s) 743, 1079, 1412, 3835, 5330, 5476
PsuI RGATCY 5 cut(s) 2742, 2770, 3389, 3550, 5251
PsyI GACNNNGTC 1 cut(s) 797
PvuII CAGCTG 1 cut(s) 4078
RseI CAYNNNNRTG 7 cut(s) 438, 551, 1565, 2237, 3648, 4017, 4770
SacI GAGCTC 6 cut(s) 201, 1243, 2141, 3765, 4134, 4521
SapI GCTCTTC 1 cut(s) 1314
Sau96I GGNCC 6 cut(s) 362, 448, 2632, 3412, 3658, 5544
SchI GAGTC 9 cut(s) 32, 497, 700, 1600, 1979, 4058, 4546, 5603, 5660
SfcI CTRYAG 8 cut(s) 974, 1302, 1619, 2879, 2954, 3353, 5486, 5762
SfuI TTCGAA 1 cut(s) 5410
SinI GGWCC 2 cut(s) 3412, 3658
SmiMI CAYNNNNRTG 7 cut(s) 438, 551, 1565, 2237, 3648, 4017, 4770
SmlI CTYRAG 8 cut(s) 695, 1094, 1160, 1242, 1368, 3398, 4351, 5213
SmoI CTYRAG 8 cut(s) 695, 1094, 1160, 1242, 1368, 3398, 4351, 5213
SspI AATATT 4 cut(s) 924, 1259, 2522, 3288
SstI GAGCTC 6 cut(s) 201, 1243, 2141, 3765, 4134, 4521
StyI CCWWGG 8 cut(s) 294, 3369, 3690, 3756, 4059, 4125, 5679, 5946
TaiI ACGT 6 cut(s) 372, 727, 2820, 3987, 4880, 5847
TatI WGTACW 6 cut(s) 177, 978, 1146, 1444, 3109, 4964
TauI GCSGC 2 cut(s) 5419, 5889
TscAI CASTG 8 cut(s) 1287, 1328, 1671, 1795, 3102, 4528, 5080, 5269
TseFI GTSAC 3 cut(s) 56, 3221, 3259
Tsp45I GTSAC 3 cut(s) 56, 3221, 3259
TspGWI ACGGA 9 cut(s) 1183, 3070, 3164, 3278, 3809, 4475, 4839, 4907, 5646
TspRI CASTG 8 cut(s) 1287, 1328, 1671, 1795, 3102, 4528, 5080, 5269
Tth111I GACNNNGTC 1 cut(s) 797
Van91I CCANNNNNTGG 1 cut(s) 3667
Vha464I CTTAAG 2 cut(s) 3398, 4351
VpaK11BI GGWCC 2 cut(s) 3412, 3658
VspI ATTAAT 1 cut(s) 3573
XagI CCTNNNNNAGG 6 cut(s) 2534, 2669, 3419, 3587, 3944, 5410
XbaI TCTAGA 3 cut(s) 769, 3229, 4261
XmnI GAANNNNTTC 5 cut(s) 306, 3736, 4105, 4494, 5069
ZraI GACGTC 1 cut(s) 370
Zsp2I ATGCAT 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.