Rh4DG011900

KIP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0006118
Physical Location & Seq
Forward (+)
1888773 .. 1897158
8386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG011900.1

Sequence Viewer

Length: 4887 bp
ATGGCAACCCTGTTACAGTCTGAGTCCAGACGCTTGTATTCTTGGTGGTGGGACAGTCACATTAGCCCAAAGAACTCAAAATGGCTTCAGGAAAATCTTACAGATATGGATGCTAAAGTCAAAGCAATGATCAAGCTCATTGAAGAAGATGCAGACTCTTTTGCAAGGAGAGCAGAAATGTACTACAAGAAACGCCCCGAGCTCATGAAACTGGTTGAGGAGTTCTATAGAGCGTATCGTGCATTGGCTGAGAGGTATGATCATGCAACTGTGGAGCTGCGCCAGGCTCATCGAACCATGGCAGAAGCATTTCCCAACCAAGTACCTTTTGAACTCGCTGATGAATCATCCTCAGGCTCTTATGGCCCAGAGGCTGGACCTCATACACCAGAAATGCTGCATCCAGTTCGTGCCTTATTTGACTCAGATGACTTGCATAAGGATGCACTGGGACTCTCTTCAACTGATTTACATGATTTGAAAAGGAATGGAGGCTCCGACTCTGGAATAAGCAAAAGAGGTCTGAAGCAGCTTAAGGAAATGTTCAATCCAGGAGAAGAGTTTAATCCAAAGGTTGCGGGGGGAAGGTTTACCGAAGGATTAAGTTTTCATGAAGCAGATGAAAGCAAACCAAAGTTACAGAATGGGTACTCTCAGTTAACTAGTGAGAACCAGAGTCTCAAGAACCAGGTCCTCTCTCAATCTGAGCGTGCTGTAAAAGCTGAAACTGAAATCCAAATCCTACAGAAAACTTTATCTGAGATACAAGCTGAAAAAGATACAGTCCATCATCAGTACCAGCAGAGTTTGGAGAAGTTATCTAAGCTGGGGAAAGAACTGAATAATGCTCAAGAGGCTGCTGGAGGGCTCAATGAACGAGCCAGTAAAGCTGATATTGAAATTACAATACTGAAGGAAGCCCTTGGGGAATTAGAAGCTGAGAGAGATGCTGGTCTTCATCAGTACAATCGGTGTCTGGAAAGGATATCTAGCCTGGAGACCATGTTATCTTTTTCCCGGGAGGATGCAAGAGGACTTAATGAGCGAGCTGTTAAAGCAGAAACTGAAGCTCAAAATATGAAGCAAGAACTTTCTAAATTAGAGGCAGAAAAGGAAGCCTGTTTTCTCAGGTACAAGCAATGTCTTGAGAAGATATCTGCTCTGGAGGCTACGATCTCAGTTGATGAAGAAAATGCCAGAATTCTTAATGAACAAATTGAAAGAGCTGAAAGTGAAGTTAAATCACTGAACGAAGTTGTTGCTATACTGAAGGAAGAGAAAGAATCTGCAGCTCTACAATTCAAGCAGTACATGGATACAATAGCTAAGATGGAAAGAGAACTTTCTCAAGCTCAAGCGGATGCTGAAAGATTGAATAATGAAATTTTGAGAGGGGCAGCAAAATTAAAGGGTGCCGAAGAACAATGTGTTCTCTTGGAGAGATCAAATCAGTCTCTGCAGTTAGAGGCAGATGGTCTGGTCAAGAAGATTGCAACAAAAGATGAAGAACTTTCAGACAAGAATGATGAGATGGAGAAACTTCATAATCTGATGCAAGAGGAGCACTTGCGGTTTGTGCAAGCTGAAGCCACTCTCCAATTTCTGCAGAAGTTGCATTCTCAATCCCAAGAAGAGCAGAAAGCTTTAGCCCTGGAGTTCAAAAACGGGCTTCAGATGTTGAAGGACTTGGAGATGAGCAAACATGGTGTGGAGGATGATATGCAACGGGTTAAGGAAGAAAATAAGAGCTTGAATGAACTGAACTTCTCTTGTACTATCTCCATTAAGAACCTGCAAGATGAAATCTTTAGCATGAAGGAGATGAAAGAGAAACTTGAAGAGGATGTTAAGCTTAAAACAGACCAGAGCAATGCTCTCCAGAGGCAAATTTCCCATTTGGAGGATGAAATTAAGGGGTTGAATGGAAGATATCAGGCTATTGTGGAGCAAGTGGAGTCAGTAGGTTTAACTCCTGAATGCTTTGGATCATCTGTGAAAGACTTGCAAAATGAGAAGTCGAGGCTAGAAGATATATGCACAAGGGATAGAGAAGACAGAGAACATCTTTACGAGAAGTTGAAGGATATGGGTAAACTTTCAAAGGAGAATGCTGTTCTGGAGAGTTCACTTTCGGGTTTGAATGGGGAGTTAGAGGGTTTGAGAGGGAAGGTCAAGGAATTGCAGGAGTCTTGCCACTTCCTCCAGGGAGAAAAAGCCACTCTTGTTGCTGAGAAATTTGCCCTGCTTTCCCAGTTGCAAATTATCACTCAGAACATGCACAAGCTTTTCGAGAAGAACTCATTGCTGGAAAGTTCCCTCTCCGGAGCAAACATTGAGCTCGAAAAGTTGAGGGCAAGAGCAAAGAGCTTAGAAGAGCTGTGCCAATTACTCAATAACGAGAAGTCCAATCTTCTTAATGAGAGAGGCATGCTGGTGTTTCAGTTAAATGATGTTGAGGAGAGACTGCGAAGCCTGGAAAAGAGGTTTAAAAAATTAGAGAAGAAGTACTCTAAAATGGAGAAGGAGAAAGAGTCCACACTTGATGCAGTAGAAGAATTACGGGGTTCCTTGCATGCAGAAAAACGAGAGCGTGCTAGTTATATTCGGTCAAGTGAATCCCGATTGGCAGGTTTGGAAAGCAATGTCCATCTCCTGCAGGAAGAAAGAAGGTTGGGAAAGAAAGAATTTGAAGCAGAACTTGACAAAGCTGTGAATGCCCAGATTGAGATCTTTATCCTTCAGAAGTTTATACAAGATCTGGAAGAGAAGAATTCGACAATATACATTGAATGTCAGAGACATGTTGAGGCTTCCAAGTTTTCTGATAAGCTGATCAAAGAGTTGGAGAGTGAAAATCTTGAGCTACAGGTGGAAGAAGAATTCTTAGTAGAAGAAATCGAAAAGTTGAGGCTGGGAATTTATCAAGTGTTCAGGTCTCTGCAAATTGAAACAGGTACACATGAAGATAAGATTGAGCGAGAAAAAGTACTTGTGCTGCACATTTTGAATGCTATCAAGGACTTGAAAAGTTCTCTGGTTCGGAGCAAGGATGAGGAGCAGCAGTTGCTGGTTGAGAATTCAGTGCTCTTTACTTTACTTCGGGAGCTGAGATCCGAAGGTGCAGAGATAGAGTCGGCAAAACAAACTTTGGAGCAGGAGTATGAGGTAATGGCAGACCATTGTGCTATGCTACAAAATGACAAGCATGAGCTTCTAGAGACGCAAAGGCAGTTGAGGTTGGAAGTGACTGAGAAAGAGCAAAAGGAGGAAATATTAGAGGCTGATTTGGAAAGTCTTCAAGGTAAGCTGGTGAGTCTGCAAGACGCATATCTGATTTTGCAGGAAGAACAATCCAAGGTGCTTGAAGAGAGGAGATCCTTGCTTAAGAAGCTTTTGGACCTGAAAGAGGAAAAGCAAGTCCTTGAAGAGGATAATAATGTTAATTTTCATGAAGCACTGGCTTTCAGCAACCTCTCTATGGTTTTAGAGAGTTTCACAATTGAGAAAGCTGTGGAACTAAAAGCACTTGCTGACGATCTTGATGGTCTTTGTGCTACTAACAATGACCTTAAAGATGCGGTTAGAACGTTGGGTGAGAAGTTAGTGATGAAAGAAGTAGAGAATCTACATCTGAATGAGATGGTTCAATTGTTGGACAAGGAACTGTGTGAAGCCAGTGACTTAAATGGTTTACTAAGCCATCAAATTTCAATTGGAAAGGATTCTCTCAAACAGAAAAACATGAAGCTCTCAGAGGTAGAAGAGAAGCTTCAAATGACAGAGGACTTGAATGTGGAATTATGCAGAACTGTTGAGGAGCTGAAGATGGAATATGAAGAGTCAAAAATAATGGGTCAAAATTATGAGAAGCAGATTCTCCAACTATCTGAAGTCAGCACTATTCAGAAAAAGGAAATGAATAGCCTGCGTGAAGCAAATGAAACTCTGGAGGATGAAATCTTATGTAAAGCGATTGAAAAAGAAATAGAAACTCTGCACCTGAGTGAGACAGCCCAATTGTTGGACATGGAATTGTGTGAAGCCAAGGACTCAAATGATCAACTGAGCCATAGAATCTTAGTTGGAGAAGGTTGTCTGAAACAGAAAACTACGGAGCTTTCAGAAGCAGAAGAAAAGCTTAGACAGACAGAAGACTTGAATGTTGAACTATGCAGAAATGTTGAGGAACTGAAGGTGCAACATGAAGAATCAAAACTCATAAGAGAAAATTGTGAGAAGGAGATTGTGGAGCTATCCAAAGATAACACAAATCAGAAAATGGAAATTGAGAGCCTTCGTGAAGCAAATGGAACTCTAGAGATTGAAGTGGGTGTCTTGTGTGAAGCAATTGAAGAATACAAAATTAGAGAAGACTATATGAGTTCAGAGCTGCAAGAAAGAAGCAATGATTTTGAACTCTGGGAGGCTGAGGCTACAACATTCTACTTTGATCTTCACAATTCTGCTGTCCGTGAAGTTTTTCTTGAAGATAAGGTTCATGAGCTTACAGAAGTCTGTGAGAGAATTAGGGATGAAGATGCTGCAAAATTTGTGGAGATTGAACAAATGAAAGAAAGAGTTAGCATCTTGGAAAGTGAAGTTGGCGGTCTGACGGCCCAGTTGTCTGCATATGCTCCTGCCATAGCCTCTTTGAGAGAGAATGTAATTTCTCTTCAGCAAAATGCCCATCTTAGGAAGAAGCTTTTCGTGGAAAACAACCAGCAACACGAGGTAATGTTCTTTCCTCTTTTAAGAAACATTAACCATCAAATGATTCAGCACTTAGAGTCTGGGCTAGGTAGAGTTGTTTTTGCTGTTGACAGCATGAAGTTACGTGGCCTGAATGTCATAGGAGAACTGTCTGTGTGTGTAGTGAAATTGATATAA

Protein Analysis

1628

Amino Acids

187.14

Weight (kDa)

4.93

Isoelectric Point (pI)

53.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 14 - 87 1.8e-38 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1800, 2618
AccB1I GGYRCC 1 cut(s) 1412
AccB7I CCANNNNNTGG 2 cut(s) 374, 4021
AccIII TCCGGA 1 cut(s) 2321
AciI CCGC 5 cut(s) 578, 1358, 1570, 3578, 4605
AclI AACGTT 1 cut(s) 3587
AclWI GGATC 3 cut(s) 1993, 3105, 3369
AfiI CCNNNNNNNGG 7 cut(s) 374, 1900, 3406, 3427, 3478, 4021, 4692
AflII CTTAAG 2 cut(s) 533, 3383
AflIII ACRYGT 1 cut(s) 2800
AhlI ACTAGT 1 cut(s) 662
AjnI CCWGG 7 cut(s) 282, 550, 687, 993, 1650, 2202, 2472
AjuI GAANNNNNNNTTGG 2 cut(s) 4584, 4616
AleI CACNNNNGTG 1 cut(s) 4002
Alw21I GWGCWC 4 cut(s) 204, 1566, 2340, 3087
Alw26I GTCTC 8 cut(s) 683, 992, 1458, 2455, 2791, 2940, 3212, 4001
AlwI GGATC 3 cut(s) 1993, 3105, 3369
AlwNI CAGNNNCTG 4 cut(s) 374, 1064, 1456, 3067
Ama87I CYCGRG 2 cut(s) 197, 1017
Aor13HI TCCGGA 1 cut(s) 2321
AoxI GGCC 3 cut(s) 364, 4614, 4837
Asp700I GAANNNNTTC 5 cut(s) 309, 3294, 3721, 4479, 4703
AspLEI GCGC 1 cut(s) 282
AspS9I GGNCC 5 cut(s) 365, 377, 691, 3397, 4615
AsuC2I CCSGG 2 cut(s) 1018, 1019
AsuHPI GGTGA 2 cut(s) 3322, 3605
AvaI CYCGRG 2 cut(s) 197, 1017
AvaII GGWCC 3 cut(s) 377, 691, 3397
AxyI CCTNAGG 1 cut(s) 352
BanI GGYRCC 1 cut(s) 1412
BanII GRGCYC 3 cut(s) 204, 870, 2340
BarI GAAGNNNNNNTAC 2 cut(s) 4656, 4688
BauI CACGAG 1 cut(s) 4727
BbsI GAAGAC 5 cut(s) 947, 2058, 3287, 4158, 4377
Bbv12I GWGCWC 4 cut(s) 204, 1566, 2340, 3087
BbvCI CCTCAGC 1 cut(s) 4428
BceAI ACGGC 1 cut(s) 4629
BcgI CGANNNNNNTGC 4 cut(s) 389, 423, 1241, 1275
BciT130I CCWGG 7 cut(s) 284, 552, 689, 995, 1652, 2204, 2474
BciVI GTATCC 1 cut(s) 1309
BclI TGATCA 4 cut(s) 129, 259, 2832, 4057
BcnI CCSGG 2 cut(s) 1018, 1019
BcoDI GTCTC 8 cut(s) 683, 992, 1458, 2455, 2791, 2940, 3212, 4001
BcuI ACTAGT 1 cut(s) 662
BfaI CTAG 7 cut(s) 663, 990, 2024, 2595, 3215, 4316, 4796
BfmI CTRYAG 7 cut(s) 226, 743, 1287, 1457, 1604, 2654, 2864
BfrI CTTAAG 2 cut(s) 533, 3383
BfuAI ACCTGC 2 cut(s) 1800, 2618
BfuI GTATCC 1 cut(s) 1309
BglII AGATCT 2 cut(s) 2727, 2755
BmcAI AGTACT 2 cut(s) 2507, 2988
Bme1390I CCNGG 9 cut(s) 284, 552, 689, 995, 1018, 1019, 1652, 2204, 2474
Bme18I GGWCC 3 cut(s) 377, 691, 3397
BmeT110I CYCGRG 2 cut(s) 197, 1017
BmgT120I GGNCC 5 cut(s) 365, 377, 691, 3397, 4615
BmiI GGNNCC 3 cut(s) 496, 1414, 2566
BmrFI CCNGG 9 cut(s) 284, 552, 689, 995, 1018, 1019, 1652, 2204, 2474
BmrI ACTGGG 3 cut(s) 458, 2245, 4612
BmuI ACTGGG 3 cut(s) 458, 2245, 4612
BpiI GAAGAC 5 cut(s) 947, 2058, 3287, 4158, 4377
BplI GAGNNNNNCTC 4 cut(s) 1858, 1890, 2282, 2314
BpmI CTGGAG 8 cut(s) 882, 1016, 1184, 1673, 1862, 2138, 2186, 3968
Bpu10I CCTNAGC 1 cut(s) 4428
BpuEI CTTGAG 6 cut(s) 665, 834, 1166, 1332, 1338, 2879
BpuMI CCSGG 2 cut(s) 1018, 1019
BsaAI YACGTR 1 cut(s) 4835
BsaBI GATNNNNATC 2 cut(s) 2726, 2732
BsaI GGTCTC 2 cut(s) 992, 2940
BsaJI CCNNGG 7 cut(s) 297, 922, 1017, 1650, 2203, 3354, 4044
BsaWI WCCGGW 1 cut(s) 2321
BsaXI ACNNNNNCTCC 8 cut(s) 989, 1019, 2516, 2546, 3047, 3077, 3143, 3173
Bsc4I CCNNNNNNNGG 7 cut(s) 374, 1900, 3406, 3427, 3478, 4021, 4692
Bse1I ACTGG 8 cut(s) 216, 404, 453, 882, 2251, 3462, 3675, 4618
Bse21I CCTNAGG 1 cut(s) 352
Bse3DI GCAATG 6 cut(s) 132, 1145, 1876, 2300, 2647, 4411
Bse8I GATNNNNATC 2 cut(s) 2726, 2732
BseAI TCCGGA 1 cut(s) 2321
BseBI CCWGG 7 cut(s) 284, 552, 689, 995, 1652, 2204, 2474
BseDI CCNNGG 7 cut(s) 297, 922, 1017, 1650, 2203, 3354, 4044
BseJI GATNNNNATC 2 cut(s) 2726, 2732
BseLI CCNNNNNNNGG 7 cut(s) 374, 1900, 3406, 3427, 3478, 4021, 4692
BseMI GCAATG 6 cut(s) 132, 1145, 1876, 2300, 2647, 4411
BseNI ACTGG 8 cut(s) 216, 404, 453, 882, 2251, 3462, 3675, 4618
BseRI GAGGAG 6 cut(s) 233, 1574, 2471, 3068, 3385, 3830
BseYI CCCAGC 2 cut(s) 826, 2911
BsgI GTGCAG 3 cut(s) 2981, 3141, 3980
BshFI GGCC 3 cut(s) 366, 4616, 4839
BshNI GGYRCC 1 cut(s) 1412
BsiHKAI GWGCWC 4 cut(s) 204, 1566, 2340, 3087
BsiHKCI CYCGRG 2 cut(s) 197, 1017
BsiSI CCGG 2 cut(s) 1018, 2322
BslFI GGGAC 2 cut(s) 65, 465
BslI CCNNNNNNNGG 7 cut(s) 374, 1900, 3406, 3427, 3478, 4021, 4692
BsmAI GTCTC 8 cut(s) 683, 992, 1458, 2455, 2791, 2940, 3212, 4001
BsmBI CGTCTC 1 cut(s) 3212
BsmFI GGGAC 2 cut(s) 65, 465
BsmI GAATGC 5 cut(s) 1615, 1982, 2113, 2719, 3013
BsnI GGCC 3 cut(s) 366, 4616, 4839
Bso31I GGTCTC 2 cut(s) 992, 2940
BsoBI CYCGRG 2 cut(s) 197, 1017
Bsp1286I GDGCHC 5 cut(s) 204, 870, 1566, 2340, 3087
Bsp13I TCCGGA 1 cut(s) 2321
Bsp19I CCATGG 1 cut(s) 297
BspACI CCGC 5 cut(s) 578, 1358, 1570, 3578, 4605
BspANI GGCC 3 cut(s) 366, 4616, 4839
BspEI TCCGGA 1 cut(s) 2321
BspHI TCATGA 4 cut(s) 204, 610, 3448, 4498
BspLI GGNNCC 3 cut(s) 496, 1414, 2566
BspMAI CTGCAG 4 cut(s) 1291, 1461, 1608, 2658
BspMI ACCTGC 2 cut(s) 1800, 2618
BspPI GGATC 3 cut(s) 1993, 3105, 3369
BspQI GCTCTTC 2 cut(s) 1626, 2367
BspT107I GGYRCC 1 cut(s) 1412
BspTI CTTAAG 2 cut(s) 533, 3383
BspTNI GGTCTC 2 cut(s) 992, 2940
BsrDI GCAATG 6 cut(s) 132, 1145, 1876, 2300, 2647, 4411
BsrI ACTGG 8 cut(s) 216, 404, 453, 882, 2251, 3462, 3675, 4618
BssECI CCNNGG 7 cut(s) 297, 922, 1017, 1650, 2203, 3354, 4044
BssSI CACGAG 1 cut(s) 4727
BssT1I CCWWGG 4 cut(s) 297, 922, 3354, 4044
Bst2BI CACGAG 1 cut(s) 4727
Bst2UI CCWGG 7 cut(s) 284, 552, 689, 995, 1652, 2204, 2474
Bst4CI ACNGT 7 cut(s) 18, 56, 271, 784, 3666, 3811, 4860
BstAFI CTTAAG 2 cut(s) 533, 3383
BstAPI GCANNNNNTGC 2 cut(s) 1612, 3064
BstBAI YACGTR 1 cut(s) 4835
BstC8I GCNNGC 7 cut(s) 711, 1047, 1581, 2429, 2574, 2592, 3926
BstDSI CCRYGG 1 cut(s) 297
BstENI CCTNNNNNAGG 2 cut(s) 3404, 3425
BstHHI GCGC 1 cut(s) 282
BstMAI GTCTC 8 cut(s) 683, 992, 1458, 2455, 2791, 2940, 3212, 4001
BstNI CCWGG 7 cut(s) 284, 552, 689, 995, 1652, 2204, 2474
BstNSI RCATGY 4 cut(s) 2278, 2431, 2576, 2804
BstSCI CCNGG 9 cut(s) 282, 550, 687, 993, 1016, 1017, 1650, 2202, 2472
BstSFI CTRYAG 7 cut(s) 226, 743, 1287, 1457, 1604, 2654, 2864
BstV2I GAAGAC 5 cut(s) 947, 2058, 3287, 4158, 4377
BstX2I RGATCY 4 cut(s) 2727, 2755, 3110, 3374
BstYI RGATCY 4 cut(s) 2727, 2755, 3110, 3374
Bsu36I CCTNAGG 1 cut(s) 352
BsuI GTATCC 1 cut(s) 1309
BsuRI GGCC 3 cut(s) 366, 4616, 4839
BtgI CCRYGG 1 cut(s) 297
BtsIMutI CAGTG 5 cut(s) 446, 1244, 3087, 3455, 3682
BveI ACCTGC 2 cut(s) 1800, 2618
Cac8I GCNNGC 7 cut(s) 711, 1047, 1581, 2429, 2574, 2592, 3926
CaiI CAGNNNCTG 4 cut(s) 374, 1064, 1456, 3067
CciI TCATGA 4 cut(s) 204, 610, 3448, 4498
CfoI GCGC 1 cut(s) 282
Cfr13I GGNCC 5 cut(s) 365, 377, 691, 3397, 4615
Cfr9I CCCGGG 1 cut(s) 1017
CseI GACGC 3 cut(s) 39, 3229, 3332
CsiI ACCWGGT 1 cut(s) 687
CspCI CAANNNNNGTGG 4 cut(s) 2206, 2241, 4533, 4568
DraI TTTAAA 1 cut(s) 2488
Ecl136II GAGCTC 2 cut(s) 202, 2338
Eco130I CCWWGG 4 cut(s) 297, 922, 3354, 4044
Eco24I GRGCYC 3 cut(s) 204, 870, 2340
Eco31I GGTCTC 2 cut(s) 992, 2940
Eco32I GATATC 3 cut(s) 987, 1155, 1931
Eco47I GGWCC 3 cut(s) 377, 691, 3397
Eco53kI GAGCTC 2 cut(s) 202, 2338
Eco81I CCTNAGG 1 cut(s) 352
Eco88I CYCGRG 2 cut(s) 197, 1017
EcoICRI GAGCTC 2 cut(s) 202, 2338
EcoNI CCTNNNNNAGG 2 cut(s) 3404, 3425
EcoO109I RGGNCCY 1 cut(s) 691
EcoRI GAATTC 4 cut(s) 1200, 2770, 2879, 3076
EcoRII CCWGG 7 cut(s) 282, 550, 687, 993, 1650, 2202, 2472
EcoRV GATATC 3 cut(s) 987, 1155, 1931
EcoT14I CCWWGG 4 cut(s) 297, 922, 3354, 4044
EcoT38I GRGCYC 3 cut(s) 204, 870, 2340
ErhI CCWWGG 4 cut(s) 297, 922, 3354, 4044
Esp3I CGTCTC 1 cut(s) 3212
FaqI GGGAC 2 cut(s) 65, 465
FauI CCCGC 1 cut(s) 571
FauNDI CATATG 1 cut(s) 4630
FbaI TGATCA 4 cut(s) 129, 259, 2832, 4057
FriOI GRGCYC 3 cut(s) 204, 870, 2340
FspBI CTAG 7 cut(s) 663, 990, 2024, 2595, 3215, 4316, 4796
GlaI GCGC 1 cut(s) 281
GsaI CCCAGC 2 cut(s) 830, 2915
GsuI CTGGAG 8 cut(s) 882, 1016, 1184, 1673, 1862, 2138, 2186, 3968
HaeIII GGCC 3 cut(s) 366, 4616, 4839
HapII CCGG 2 cut(s) 1018, 2322
HgaI GACGC 3 cut(s) 39, 3229, 3332
HhaI GCGC 1 cut(s) 282
Hin6I GCGC 1 cut(s) 280
HinP1I GCGC 1 cut(s) 280
HincII GTYRAC 2 cut(s) 660, 4819
HindII GTYRAC 2 cut(s) 660, 4819
HindIII AAGCTT 7 cut(s) 1641, 1850, 2282, 3389, 3767, 4136, 4700
HpaI GTTAAC 1 cut(s) 660
HpaII CCGG 2 cut(s) 1018, 2322
HphI GGTGA 2 cut(s) 3322, 3605
Hpy166II GTNNAC 8 cut(s) 591, 660, 2093, 2126, 2535, 2957, 3692, 4819
Hpy8I GTNNAC 8 cut(s) 591, 660, 2093, 2126, 2535, 2957, 3692, 4819
HpyCH4III ACNGT 7 cut(s) 18, 56, 271, 784, 3666, 3811, 4860
HpyCH4IV ACGT 2 cut(s) 3587, 4834
HpySE526I ACGT 2 cut(s) 3587, 4834
HspAI GCGC 1 cut(s) 280
Kpn2I TCCGGA 1 cut(s) 2321
Ksp22I TGATCA 4 cut(s) 129, 259, 2832, 4057
KspAI GTTAAC 1 cut(s) 660
LguI GCTCTTC 2 cut(s) 1626, 2367
MabI ACCWGGT 1 cut(s) 687
MaeI CTAG 7 cut(s) 663, 990, 2024, 2595, 3215, 4316, 4796
MaeII ACGT 2 cut(s) 3587, 4834
MaeIII GTNAC 6 cut(s) 12, 56, 636, 3244, 3677, 4830
MfeI CAATTG 5 cut(s) 3498, 3647, 3711, 4016, 4347
MflI RGATCY 4 cut(s) 2727, 2755, 3110, 3374
MhlI GDGCHC 5 cut(s) 204, 870, 1566, 2340, 3087
MmeI TCCRAC 7 cut(s) 522, 2823, 3219, 3633, 3904, 4002, 4063
MroI TCCGGA 1 cut(s) 2321
MroXI GAANNNNTTC 5 cut(s) 309, 3294, 3721, 4479, 4703
MslI CAYNNNNRTG 4 cut(s) 441, 2432, 3633, 4002
MspCI CTTAAG 2 cut(s) 533, 3383
MspI CCGG 2 cut(s) 1018, 2322
MspR9I CCNGG 9 cut(s) 284, 552, 689, 995, 1018, 1019, 1652, 2204, 2474
MunI CAATTG 5 cut(s) 3498, 3647, 3711, 4016, 4347
Mva1269I GAATGC 5 cut(s) 1615, 1982, 2113, 2719, 3013
MvaI CCWGG 7 cut(s) 284, 552, 689, 995, 1652, 2204, 2474
NciI CCSGG 2 cut(s) 1018, 1019
NcoI CCATGG 1 cut(s) 297
NdeI CATATG 1 cut(s) 4630
NlaIV GGNNCC 3 cut(s) 496, 1414, 2566
NmuCI GTSAC 3 cut(s) 56, 3244, 3677
NspI RCATGY 4 cut(s) 2278, 2431, 2576, 2804
OliI CACNNNNGTG 1 cut(s) 4002
PaeI GCATGC 2 cut(s) 2431, 2576
PagI TCATGA 4 cut(s) 204, 610, 3448, 4498
PciI ACATGT 1 cut(s) 2800
PciSI GCTCTTC 2 cut(s) 1626, 2367
PctI GAATGC 5 cut(s) 1615, 1982, 2113, 2719, 3013
PdmI GAANNNNTTC 5 cut(s) 309, 3294, 3721, 4479, 4703
PfeI GAWTC 9 cut(s) 344, 1283, 2615, 3622, 3722, 3874, 4074, 4208, 4774
PflMI CCANNNNNTGG 2 cut(s) 374, 4021
PfoI TCCNGGA 1 cut(s) 550
Ppu21I YACGTR 1 cut(s) 4835
PpuMI RGGWCCY 1 cut(s) 691
PscI ACATGT 1 cut(s) 2800
Psp124BI GAGCTC 2 cut(s) 204, 2340
Psp1406I AACGTT 1 cut(s) 3587
Psp5II RGGWCCY 1 cut(s) 691
Psp6I CCWGG 7 cut(s) 282, 550, 687, 993, 1650, 2202, 2472
PspFI CCCAGC 2 cut(s) 826, 2911
PspGI CCWGG 7 cut(s) 282, 550, 687, 993, 1650, 2202, 2472
PspN4I GGNNCC 3 cut(s) 496, 1414, 2566
PspPI GGNCC 5 cut(s) 365, 377, 691, 3397, 4615
PspPPI RGGWCCY 1 cut(s) 691
PstI CTGCAG 4 cut(s) 1291, 1461, 1608, 2658
PstNI CAGNNNCTG 4 cut(s) 374, 1064, 1456, 3067
PsuI RGATCY 4 cut(s) 2727, 2755, 3110, 3374
RseI CAYNNNNRTG 4 cut(s) 441, 2432, 3633, 4002
SacI GAGCTC 2 cut(s) 204, 2340
SapI GCTCTTC 2 cut(s) 1626, 2367
Sau96I GGNCC 5 cut(s) 365, 377, 691, 3397, 4615
SbfI CCTGCAGG 1 cut(s) 2658
ScaI AGTACT 2 cut(s) 2507, 2988
ScrFI CCNGG 9 cut(s) 284, 552, 689, 995, 1018, 1019, 1652, 2204, 2474
SdaI CCTGCAGG 1 cut(s) 2658
SduI GDGCHC 5 cut(s) 204, 870, 1566, 2340, 3087
SexAI ACCWGGT 1 cut(s) 687
SfcI CTRYAG 7 cut(s) 226, 743, 1287, 1457, 1604, 2654, 2864
SinI GGWCC 3 cut(s) 377, 691, 3397
SmaI CCCGGG 1 cut(s) 1019
SmiMI CAYNNNNRTG 4 cut(s) 441, 2432, 3633, 4002
SmlI CTYRAG 8 cut(s) 533, 680, 849, 1145, 1347, 1353, 2858, 3383
SmoI CTYRAG 8 cut(s) 533, 680, 849, 1145, 1347, 1353, 2858, 3383
SpeI ACTAGT 1 cut(s) 662
SphI GCATGC 2 cut(s) 2431, 2576
Sse8387I CCTGCAGG 1 cut(s) 2658
SsiI CCGC 5 cut(s) 578, 1358, 1570, 3578, 4605
SspI AATATT 1 cut(s) 3273
SspMI CTAG 7 cut(s) 663, 990, 2024, 2595, 3215, 4316, 4796
SstI GAGCTC 2 cut(s) 204, 2340
StyD4I CCNGG 9 cut(s) 282, 550, 687, 993, 1016, 1017, 1650, 2202, 2472
StyI CCWWGG 4 cut(s) 297, 922, 3354, 4044
TaaI ACNGT 7 cut(s) 18, 56, 271, 784, 3666, 3811, 4860
TaiI ACGT 2 cut(s) 3590, 4837
TaqI TCGA 6 cut(s) 292, 2018, 2289, 2340, 2774, 2898
TaqII GACCGA 1 cut(s) 2595
TatI WGTACW 6 cut(s) 180, 963, 1308, 1772, 2505, 2986
TfiI GAWTC 9 cut(s) 344, 1283, 2615, 3622, 3722, 3874, 4074, 4208, 4774
TscAI CASTG 5 cut(s) 453, 1251, 3087, 3462, 3682
TseFI GTSAC 3 cut(s) 56, 3244, 3677
Tsp45I GTSAC 3 cut(s) 56, 3244, 3677
TspGWI ACGGA 2 cut(s) 4127, 4460
TspMI CCCGGG 1 cut(s) 1017
TspRI CASTG 5 cut(s) 453, 1251, 3087, 3462, 3682
Van91I CCANNNNNTGG 2 cut(s) 374, 4021
Vha464I CTTAAG 2 cut(s) 533, 3383
VpaK11BI GGWCC 3 cut(s) 377, 691, 3397
XagI CCTNNNNNAGG 2 cut(s) 3404, 3425
XbaI TCTAGA 2 cut(s) 3214, 4315
XceI RCATGY 4 cut(s) 2278, 2431, 2576, 2804
XmaI CCCGGG 1 cut(s) 1017
XmnI GAANNNNTTC 5 cut(s) 309, 3294, 3721, 4479, 4703
XspI CTAG 7 cut(s) 663, 990, 2024, 2595, 3215, 4316, 4796
ZrmI AGTACT 2 cut(s) 2507, 2988
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.