pycom13g17150

KIP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0006118
Physical Location & Seq
Forward (+)
12898703 .. 12903940
5238 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g17150.1

Sequence Viewer

Length: 4491 bp
ATGGCAACCATGTTACATTCTGAGTCCAGACGATTATATTCTTGGTGGTGGGATAGTCACATTCCAAAGAACTCAAAATGGCTTCAGGAGAATCTTACAGACATGGATGCCAAAGTCAAAGCAATGATCGATCTCATTGAAGAAGATGCAGATTCTTTTGCAAGGAGGGCAGAAATGTACTACAAGAAACGCCCGGAGCTCATGAAACTGGTGGAGGAGTTCTATCGAGCTTATCGTGCATTAGCAGAGAGGTACGATCATGCAACTGTGGAGCTGCGCCAGGCTCATCGAACCATTGCAGTAGCATTTCCCATCCATGTACCTTATGTATTGGCTGATGAATCGTCCTCAGGCTCTTCTGGGCCAGACGTCGAACCTCATACACCAGAAATGCCACATCCAGTTTGTGCCTTATTTGACCCTGATGACTTGCATAAGGATGCATTGGGCCTCTCGTCAATCAATTCACAAGCTTTGAAAAGGAATGGAGGAAACTCATCAGACTCCGACTCTGGAATAAGCAGAAGAGGTGGTCTAAAGCAGTTCATTGAGATGTTCACTCCAGGAGAAGTGCCAAATAGTTCAAAGGTTGCAGAGGGAATGATGGGACAAGGATTAAATTTTCACGAAGCGGAAGATTTCAAACAGAAGTTCCAAAATGGGTTTTCTCAGTTAACGAGTGAGAACCAGAGTCTCAAGACCCAGGTTCTTTCTCAATCTGAACGTGCTGCAAAAGCAGAAACTGAAGTTCAATCCTTAAAGAAAGCTCTAGATGAGATACAATCTGAAAAAGACGCAGTCCTTCTTCAGTATGAGCAGAGTTTGGAGCAGTTGTCTAAACTGGGGAGAGAACTAGATGATGCACAAAGGGCTATTGGAGGGCTTGATGAACGAGCAAGCAAAGCTGATATTGAAACTAAAATATTGAAGGAAGCCCTGGTGGAATTAGAAGCGGAGAGGGATGCTGGTCTTCTGCAGTACAATCGATGTTTGGAAAGGATATCTAACCTGGAGAGCAAGTTATCTTTTTCCCAATGGGATGCAAAGGGACTTAACGAGCGGGCTGTTAAAGCAGAAACTGAAGCTCAAAGTCTCAAGCAAGAACTTTCTAAACTAGAGGTTGAAAAGGAAGATTGTTTACTTCAGTACAAGCAATGTCTTGAGAAGATATCTGCTTTGGAGACGAAAATATCTGTCTCTGAGGAAAATGTCAGAATGCTTAATGAACAAATTGAAAGAGCTCAAGGTGAAGTCAAATATTTGAAGGAATCCCTGGCTACACTGGAGGAAGGGAAAGAAGCTGCAGCTCTTCTATACAAGCAGTGCATGGATACAATAGCTAAAATGGAAAGCGAAATTTCTGAGGCTCAAGCAGATGCCAAACGACTAAATAGCGAAGTTTTGACAGGCTCCGCAAAATTAAAGAGTGCCGAAGAACAATGTGTACGCTTGGAGAGATCAAATCACTCTCTGCGGTTAGAGGCGGACAGTCTGCTGAAGAAGATTACAACTAAGGATCAAGAACTTTCAGAGAAGGATGACCAGATGGAGAAACTTCACATTTTGATGGAAGAGGAGCACTTACAGTTTGTGCAAGCTGAAGCGACTCTTCAAGCTCTACAGAAGTTGCATTCTCAATCCCAGGAGGAACAGAAAGCTTTAGCACTGGAGTTCAAAAATGGGCTTCAAATGTTGAAGGACTTGGAGATACGCGAACAGGGTATGAAGGATGATGTCCAACGGGCAAAGGAGGAAAATAAGAGCCTAAGTGAATTGAATTTTTCTTGCACTGTCTCCATAAAGAATCTGCAAGATGAAATCTTCAACATCAAAGAGATGAAAGAAAAACTTGAACAAGAGGTTGCGGTAAAATCAGACCAAAGCAATGCTCTCCAGCAGCATATTTTACATTTGGAGGAGGAAATTAAGGGATTAAATAGAAGATATCAAGCTATGGTGAAGCAGGTGGAGTCGGCTGGTTTAAATCCTGAATGCTTTGAATCATCTGTGAAAGACTTGCAAAATGAAAAATCGAAGCTGGAAGATATATGCACAAGGGGGAAAGAAGAGAGAGAACTTCTTTATGAGAAGTTGAAGGATACGGGTAAACTTTCAAAGGAGAACGATTTTATGGGGAGCTCACTGTTGGGGTTGAACGCTGAGTTGGAGGGTTTGAGAGAGAAGGTCCAAGAATTGCAAGCGTCTTGCCAGTTTCTCCAGGGAGAAAAATCCACTCTTGTGGCTGAGAAGGCTCTCCTGCTTTCACAGTTACAAGTTATCACTCAGAATATGCAGGCGCTTTTTGAGAGAAACACCTTGCTGGACAATTCCCTCTCTGTTGCAGACATTGAGCTTGAACGGTTCAGAGCACGATCAAACAGCTTAGAAGAGTTGTGCCAATCTCTCAATAATGAGAAGTCCAATCTTCTTAATGAGAGAGGCACCCTGGTATTTCAGTTGAAAGATGTTGAGGAGAGACTGCGAAACCTGGAAAAGCGCTTTACAAAATTAGAGAAGAAATATTCTAACCTGGGGAAGGAGGAAGGCTCCACACTTAGTGCAGTAGAAGAACTACGGGGTTCCCTCCATGCAGAAAAACGAGAGCGTGCAAGTTATATACGGTCAAGTGAGGCCCGATTGGCGGGTTTGGAAAACAATGTCCACCTCATGCAGGAAGAAAGAAAGCTGGGAAAGAAAGAATTTGAAGAAGAACTAGATAAAGCTTTAAATGCCCAGATCGAGATCTTTATCCTTCAGAAGTTTATAGAAGATCTGGAAGAGAAGAATTTGTCATTATTCATCGAGTGTCAGAGACACGTTGAGAAATCCAAGTTTTCTAGTAAGCTGATCTCAGAGTTGGAGAGTGAAAATCTCGAACTACAGGTGGAAGAACAATTCTTGGTGGAAGAAATCGACAAGTTGAGGTTGGGCGTTCGTCAAGTCCTCAGGGCTCTACAGATTGAACCAGATAGGCAGGACGATAAGACTGAACCAGGGAAAGTAAATGTGCTGCACATTTTGAATACTATCAAGGACTTGAAAACTTCTTTGTTACGGAGCAAGGATGAGGAACAGCAGTTGCTTGTTGAGAAGTCAGTGCTCTTAACTGTACTTGGGCAGTTGAGATCAGAGGGTGCAGAGGTAGAGACGGAAAAACAATTCTTTGAGCAGGAGTATGAGATCGTGATAAACCATTGTTCTACACTACAAAATGAGAAGCATGACCTTCTAATGATGACAAGGGAGTTGAGATTGGAAGTGACGGGGAAAGAGCAAAAGGAGGAAATATTAGAGGCTGAATTGTTAACTCTTCAACCTAAGCTGACAAATTTGGAGGATGCTTATGTGGTTCTGCAGGAAGAAAATTCCAAGGTGCTTGAAGAGAAGAGATCTTTGCTTAAGGAACTTATGGCCCTCAAAGAGGAAAAGAAAATGCTTGAAGAGGATAGTAGTGTTAATTTCCACGAAGCACTAGCTTTCAGCACCCTCTCGTTGGTTTTAGAGAGCTTCACAATTGAGAAAGCTGCGGAACTAAAATCACTTGCTAAAGATCTCAACAGTCAATTTGTAATTAATAACGACCTCAAAGAGGCTGTTGGAACATTGGGGGAGGATTTAGTGATGAAAGAAGTAGAAAACCTGCATCTGAATGAGACGGTCCAATTGTTGGACAAAGAACTGCGTGAAGCCAAGGACTTAAATGGTGAACTAAGCCGTCAAGTTTTAGTTGGAAACGATTCTCTGAAACAGAAAACCTTGGAGCTCTCGGAGGCAGAAGAGAAGCTTAAAAGGATGGAAGATTTGAATGTGGAATTGTGCAGAACAGTTCAGGAACTGAAGATGGAAATTGAAGAATCAAATCTAGTGCGAGAAAATTGTGAGAAGGAGATTTTTGAACTATCTGAAGATAGCACAAATCAGAAAAAGGAAATCAAAAGCCTTTGTGAGGCAAACGAAATTCTGAAGAACCAAATCTTATGTAACGGAATTGAAAAAGAAGTAGAAAATCTACATCTGAATGAGACAGTTCGATTGTTGCACAAGGAACTGCATGAAGCCAAGGACTCGAAAGATCAGCTGAACCATCAAATTTTAGCTGGAAAGGATTCTCTGAAGCAGAAAACCGTGGAGCTCTCAGAAGTAGAAGAGAAGCTTAGAAAGGCTGAAAACTTGAATGTGGAATTATGCAGAACTGTTCAGGAAATGAGGATGGAACATGAAGATTCAAAGCGACTGAGGGAGAATTGTGAGAAGCAGATTCTAGAACTATCCAATGATAACTCAAATCAGAAAGATGAAATCGACAGCCTTCGTGAAGCAAATGAAACCCTGGCGAATGAAGTGGGTATCTTAAGTGAAGTAATTGAAGAACATAGAACAAGAGAGGAGTATTTGAGTTCCGAGCTGCAAGAGAGAAGCAATGACTTTGAACTCTGGGAGGCTGAGGCTGCGGCATTCTATTTTGATCTATCAAGTTTCTGCTCTCCGTGA

Protein Analysis

1497

Amino Acids

171.68

Weight (kDa)

4.9

Isoelectric Point (pI)

53.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 14 - 86 2.8e-36 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1954
AatII GACGTC 1 cut(s) 372
Acc36I ACCTGC 2 cut(s) 1954, 3648
AccB1I GGYRCC 1 cut(s) 2441
AccB7I CCANNNNNTGG 1 cut(s) 3667
AccBSI CCGCTC 1 cut(s) 1060
AccII CGCG 1 cut(s) 1713
AclWI GGATC 1 cut(s) 1524
AcsI RAATTY 9 cut(s) 619, 1356, 1777, 2699, 2785, 3328, 3364, 3957, 4089
AcyI GRCGYC 1 cut(s) 369
AdeI CACNNNGTG 1 cut(s) 2558
AfaI GTAC 7 cut(s) 179, 254, 321, 980, 1148, 1446, 3111
AfeI AGCGCT 1 cut(s) 2498
AfiI CCNNNNNNNGG 8 cut(s) 2536, 2641, 2671, 3421, 3493, 3589, 3667, 3946
AflII CTTAAG 2 cut(s) 3398, 4351
AflIII ACRYGT 1 cut(s) 2815
AjuI GAANNNNNNNTTGG 2 cut(s) 2147, 2179
AleI CACNNNNGTG 1 cut(s) 2237
Alw21I GWGCWC 8 cut(s) 201, 1243, 1581, 2141, 2371, 3102, 3765, 4134
AlwI GGATC 1 cut(s) 1524
AlwNI CAGNNNCTG 3 cut(s) 743, 1079, 3835
Aor51HI AGCGCT 1 cut(s) 2498
AoxI GGCC 4 cut(s) 362, 448, 2631, 3411
ApeKI GCWGC 9 cut(s) 274, 728, 1301, 1304, 1897, 3010, 3524, 4405, 4448
ApoI RAATTY 9 cut(s) 619, 1356, 1777, 2699, 2785, 3328, 3364, 3957, 4089
AseI ATTAAT 1 cut(s) 3573
Asp700I GAANNNNTTC 2 cut(s) 3736, 4105
AspLEI GCGC 3 cut(s) 279, 2299, 2499
AspS9I GGNCC 6 cut(s) 362, 448, 2185, 2632, 3412, 3658
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 3 cut(s) 1259, 1969, 3716
AvaII GGWCC 2 cut(s) 2185, 3658
AxyI CCTNAGG 2 cut(s) 349, 2945
BanI GGYRCC 1 cut(s) 2441
BanII GRGCYC 6 cut(s) 201, 1243, 2141, 2953, 3765, 4134
BarI GAAGNNNNNNTAC 2 cut(s) 1122, 1154
BbsI GAAGAC 1 cut(s) 962
Bbv12I GWGCWC 8 cut(s) 201, 1243, 1581, 2141, 2371, 3102, 3765, 4134
BbvCI CCTCAGC 1 cut(s) 4443
BbvI GCAGC 9 cut(s) 261, 715, 1288, 1316, 1909, 2997, 3511, 4392, 4435
BccI CCATC 8 cut(s) 320, 598, 1540, 1561, 3787, 3835, 4092, 4204
BceAI ACGGC 1 cut(s) 3699
BcgI CGANNNNNNTGC 2 cut(s) 965, 999
BciVI GTATCC 2 cut(s) 1324, 2092
BcnI CCSGG 1 cut(s) 194
BfaI CTAG 8 cut(s) 770, 854, 1115, 2714, 2838, 3473, 3863, 4262
BfmI CTRYAG 6 cut(s) 974, 1302, 1619, 2879, 2954, 3353
BfoI RGCGCY 2 cut(s) 2300, 2500
BfrI CTTAAG 2 cut(s) 3398, 4351
BfuAI ACCTGC 2 cut(s) 1954, 3648
BfuI GTATCC 2 cut(s) 1324, 2092
BglI GCCNNNNNGGC 1 cut(s) 2639
BglII AGATCT 4 cut(s) 2742, 2770, 3389, 3550
Bme18I GGWCC 2 cut(s) 2185, 3658
BmgT120I GGNCC 6 cut(s) 362, 448, 2185, 2632, 3412, 3658
BmiI GGNNCC 4 cut(s) 1411, 2443, 2548, 2581
BmrI ACTGGG 1 cut(s) 851
BmsI GCATC 9 cut(s) 97, 136, 430, 850, 952, 1030, 1366, 3328, 3652
BmuI ACTGGG 1 cut(s) 851
BpiI GAAGAC 1 cut(s) 962
BplI GAGNNNNNCTC 2 cut(s) 2531, 2563
BpmI CTGGAG 6 cut(s) 546, 1031, 1304, 1688, 1877, 2201
Bpu10I CCTNAGC 2 cut(s) 3318, 4443
BpuEI CTTGAG 5 cut(s) 680, 1079, 1181, 1227, 1353
BpuMI CCSGG 1 cut(s) 194
Bsa29I ATCGAT 2 cut(s) 129, 985
BsaBI GATNNNNATC 2 cut(s) 2741, 2747
BsaHI GRCGYC 1 cut(s) 369
BsaXI ACNNNNNCTCC 4 cut(s) 818, 848, 1004, 1034
Bsc4I CCNNNNNNNGG 8 cut(s) 2536, 2641, 2671, 3421, 3493, 3589, 3667, 3946
Bse1I ACTGG 6 cut(s) 213, 401, 846, 1287, 1671, 2209
Bse21I CCTNAGG 2 cut(s) 349, 2945
Bse3DI GCAATG 5 cut(s) 129, 294, 1160, 1891, 4426
Bse8I GATNNNNATC 2 cut(s) 2741, 2747
BseCI ATCGAT 2 cut(s) 129, 985
BseJI GATNNNNATC 2 cut(s) 2741, 2747
BseLI CCNNNNNNNGG 8 cut(s) 2536, 2641, 2671, 3421, 3493, 3589, 3667, 3946
BseMI GCAATG 5 cut(s) 129, 294, 1160, 1891, 4426
BseNI ACTGG 6 cut(s) 213, 401, 846, 1287, 1671, 2209
BseRI GAGGAG 5 cut(s) 230, 1589, 1931, 2486, 4400
BseXI GCAGC 9 cut(s) 261, 715, 1288, 1316, 1909, 2997, 3511, 4392, 4435
BseYI CCCAGC 1 cut(s) 2686
BsgI GTGCAG 4 cut(s) 2580, 2996, 3156, 3838
Bsh1236I CGCG 1 cut(s) 1713
BshFI GGCC 4 cut(s) 364, 450, 2633, 3413
BshNI GGYRCC 1 cut(s) 2441
BshVI ATCGAT 2 cut(s) 129, 985
BsiHKAI GWGCWC 8 cut(s) 201, 1243, 1581, 2141, 2371, 3102, 3765, 4134
BsiSI CCGG 1 cut(s) 194
BslFI GGGAC 2 cut(s) 621, 1062
BslI CCNNNNNNNGG 8 cut(s) 2536, 2641, 2671, 3421, 3493, 3589, 3667, 3946
BsmBI CGTCTC 3 cut(s) 1175, 3140, 3647
BsmFI GGGAC 2 cut(s) 621, 1062
BsmI GAATGC 4 cut(s) 1221, 1630, 1997, 4454
BsnI GGCC 4 cut(s) 364, 450, 2633, 3413
Bsp1286I GDGCHC 9 cut(s) 201, 1243, 1581, 2141, 2371, 2953, 3102, 3765, 4134
BspANI GGCC 4 cut(s) 364, 450, 2633, 3413
BspDI ATCGAT 2 cut(s) 129, 985
BspFNI CGCG 1 cut(s) 1713
BspHI TCATGA 1 cut(s) 201
BspLI GGNNCC 4 cut(s) 1411, 2443, 2548, 2581
BspMAI CTGCAG 3 cut(s) 978, 1306, 3357
BspMI ACCTGC 2 cut(s) 1954, 3648
BspPI GGATC 1 cut(s) 1524
BspQI GCTCTTC 2 cut(s) 361, 1314
BspT107I GGYRCC 1 cut(s) 2441
BspTI CTTAAG 2 cut(s) 3398, 4351
BsrBI CCGCTC 1 cut(s) 1060
BsrDI GCAATG 5 cut(s) 129, 294, 1160, 1891, 4426
BsrI ACTGG 6 cut(s) 213, 401, 846, 1287, 1671, 2209
BssNI GRCGYC 1 cut(s) 369
BssT1I CCWWGG 4 cut(s) 3369, 3690, 3756, 4059
BstACI GRCGYC 1 cut(s) 369
BstAFI CTTAAG 2 cut(s) 3398, 4351
BstC8I GCNNGC 6 cut(s) 898, 1062, 1596, 2199, 2295, 2607
BstDSI CCRYGG 1 cut(s) 4125
BstENI CCTNNNNNAGG 5 cut(s) 2534, 2669, 3419, 3587, 3944
BstFNI CGCG 1 cut(s) 1713
BstH2I RGCGCY 2 cut(s) 2300, 2500
BstHHI GCGC 3 cut(s) 279, 2299, 2499
BstSFI CTRYAG 6 cut(s) 974, 1302, 1619, 2879, 2954, 3353
BstUI CGCG 1 cut(s) 1713
BstV1I GCAGC 9 cut(s) 261, 715, 1288, 1316, 1909, 2997, 3511, 4392, 4435
BstV2I GAAGAC 1 cut(s) 962
BstX2I RGATCY 4 cut(s) 2742, 2770, 3389, 3550
BstXI CCANNNNNNTGG 1 cut(s) 2239
BstYI RGATCY 4 cut(s) 2742, 2770, 3389, 3550
Bsu15I ATCGAT 2 cut(s) 129, 985
Bsu36I CCTNAGG 2 cut(s) 349, 2945
BsuI GTATCC 2 cut(s) 1324, 2092
BsuRI GGCC 4 cut(s) 364, 450, 2633, 3413
BsuTUI ATCGAT 2 cut(s) 129, 985
BtgI CCRYGG 1 cut(s) 4125
BtsI GCAGTG 1 cut(s) 1328
BtsIMutI CAGTG 6 cut(s) 1280, 1328, 1664, 1788, 2141, 3102
BveI ACCTGC 2 cut(s) 1954, 3648
Cac8I GCNNGC 6 cut(s) 898, 1062, 1596, 2199, 2295, 2607
CaiI CAGNNNCTG 3 cut(s) 743, 1079, 3835
CciI TCATGA 1 cut(s) 201
CfoI GCGC 3 cut(s) 279, 2299, 2499
Cfr13I GGNCC 6 cut(s) 362, 448, 2185, 2632, 3412, 3658
ClaI ATCGAT 2 cut(s) 129, 985
CseI GACGC 2 cut(s) 803, 2190
Csp6I GTAC 7 cut(s) 178, 253, 320, 979, 1147, 1445, 3110
CviQI GTAC 7 cut(s) 178, 253, 320, 979, 1147, 1445, 3110
DraI TTTAAA 2 cut(s) 1983, 2727
DraIII CACNNNGTG 1 cut(s) 2558
EciI GGCGGA 1 cut(s) 1499
Ecl136II GAGCTC 5 cut(s) 199, 1241, 2139, 3763, 4132
Eco130I CCWWGG 4 cut(s) 3369, 3690, 3756, 4059
Eco24I GRGCYC 6 cut(s) 201, 1243, 2141, 2953, 3765, 4134
Eco32I GATATC 3 cut(s) 1002, 1170, 1946
Eco47I GGWCC 2 cut(s) 2185, 3658
Eco47III AGCGCT 1 cut(s) 2498
Eco53kI GAGCTC 5 cut(s) 199, 1241, 2139, 3763, 4132
Eco81I CCTNAGG 2 cut(s) 349, 2945
EcoICRI GAGCTC 5 cut(s) 199, 1241, 2139, 3763, 4132
EcoNI CCTNNNNNAGG 5 cut(s) 2534, 2669, 3419, 3587, 3944
EcoRV GATATC 3 cut(s) 1002, 1170, 1946
EcoT14I CCWWGG 4 cut(s) 3369, 3690, 3756, 4059
EcoT22I ATGCAT 1 cut(s) 445
EcoT38I GRGCYC 6 cut(s) 201, 1243, 2141, 2953, 3765, 4134
ErhI CCWWGG 4 cut(s) 3369, 3690, 3756, 4059
Esp3I CGTCTC 3 cut(s) 1175, 3140, 3647
FalI AAGNNNNNCTT 8 cut(s) 1593, 1625, 1833, 1865, 3768, 3800, 4137, 4169
FaqI GGGAC 2 cut(s) 621, 1062
FauI CCCGC 2 cut(s) 1053, 2635
FriOI GRGCYC 6 cut(s) 201, 1243, 2141, 2953, 3765, 4134
FspBI CTAG 8 cut(s) 770, 854, 1115, 2714, 2838, 3473, 3863, 4262
GlaI GCGC 3 cut(s) 278, 2298, 2498
GsaI CCCAGC 1 cut(s) 2690
GsuI CTGGAG 6 cut(s) 546, 1031, 1304, 1688, 1877, 2201
HaeII RGCGCY 2 cut(s) 2300, 2500
HaeIII GGCC 4 cut(s) 364, 450, 2633, 3413
HapII CCGG 1 cut(s) 194
HgaI GACGC 2 cut(s) 803, 2190
HhaI GCGC 3 cut(s) 279, 2299, 2499
Hin1I GRCGYC 1 cut(s) 369
Hin6I GCGC 3 cut(s) 277, 2297, 2497
HinP1I GCGC 3 cut(s) 277, 2297, 2497
HincII GTYRAC 2 cut(s) 675, 3306
HindII GTYRAC 2 cut(s) 675, 3306
HindIII AAGCTT 5 cut(s) 471, 1656, 2721, 3782, 4151
HpaI GTTAAC 2 cut(s) 675, 3306
HpaII CCGG 1 cut(s) 194
HphI GGTGA 3 cut(s) 1259, 1969, 3716
Hpy166II GTNNAC 8 cut(s) 558, 675, 1139, 1445, 2108, 2662, 3306, 3707
Hpy8I GTNNAC 8 cut(s) 558, 675, 1139, 1445, 2108, 2662, 3306, 3707
Hpy99I CGWCG 1 cut(s) 374
HpyCH4IV ACGT 3 cut(s) 369, 724, 2817
HpySE526I ACGT 3 cut(s) 369, 724, 2817
Hsp92I GRCGYC 1 cut(s) 369
HspAI GCGC 3 cut(s) 277, 2297, 2497
KspAI GTTAAC 2 cut(s) 675, 3306
LguI GCTCTTC 2 cut(s) 361, 1314
Lsp1109I GCAGC 9 cut(s) 261, 715, 1288, 1316, 1909, 2997, 3511, 4392, 4435
LweI GCATC 9 cut(s) 97, 136, 430, 850, 952, 1030, 1366, 3328, 3652
MaeI CTAG 8 cut(s) 770, 854, 1115, 2714, 2838, 3473, 3863, 4262
MaeII ACGT 3 cut(s) 369, 724, 2817
MaeIII GTNAC 6 cut(s) 12, 56, 2268, 3051, 3259, 3980
MbiI CCGCTC 1 cut(s) 1060
MfeI CAATTG 2 cut(s) 3513, 3662
MflI RGATCY 4 cut(s) 2742, 2770, 3389, 3550
MhlI GDGCHC 9 cut(s) 201, 1243, 1581, 2141, 2371, 2953, 3102, 3765, 4134
MlyI GAGTC 7 cut(s) 32, 497, 503, 700, 1600, 1979, 4058
MmeI TCCRAC 7 cut(s) 531, 1762, 2145, 2838, 3577, 3648, 3709
Mph1103I ATGCAT 1 cut(s) 445
MroXI GAANNNNTTC 2 cut(s) 3736, 4105
MslI CAYNNNNRTG 6 cut(s) 438, 551, 1565, 2237, 3648, 4017
MspA1I CMGCKG 1 cut(s) 4078
MspCI CTTAAG 2 cut(s) 3398, 4351
MspI CCGG 1 cut(s) 194
MunI CAATTG 2 cut(s) 3513, 3662
Mva1269I GAATGC 4 cut(s) 1221, 1630, 1997, 4454
MvnI CGCG 1 cut(s) 1713
NciI CCSGG 1 cut(s) 194
NlaIV GGNNCC 4 cut(s) 1411, 2443, 2548, 2581
NmuCI GTSAC 2 cut(s) 56, 3259
NsiI ATGCAT 1 cut(s) 445
OliI CACNNNNGTG 1 cut(s) 2237
PagI TCATGA 1 cut(s) 201
PaqCI CACCTGC 1 cut(s) 1954
PciSI GCTCTTC 2 cut(s) 361, 1314
PcsI WCGNNNNNNNCGW 1 cut(s) 232
PctI GAATGC 4 cut(s) 1221, 1630, 1997, 4454
PdmI GAANNNNTTC 2 cut(s) 3736, 4105
PflFI GACNNNGTC 1 cut(s) 797
PflMI CCANNNNNTGG 1 cut(s) 3667
PfoI TCCNGGA 1 cut(s) 562
PleI GAGTC 7 cut(s) 31, 497, 503, 699, 1600, 1978, 4058
PpsI GAGTC 7 cut(s) 31, 497, 503, 699, 1600, 1978, 4058
PshBI ATTAAT 1 cut(s) 3573
Psp124BI GAGCTC 5 cut(s) 201, 1243, 2141, 3765, 4134
PspFI CCCAGC 1 cut(s) 2686
PspN4I GGNNCC 4 cut(s) 1411, 2443, 2548, 2581
PspPI GGNCC 6 cut(s) 362, 448, 2185, 2632, 3412, 3658
PsrI GAACNNNNNNTAC 2 cut(s) 1428, 1460
PstI CTGCAG 3 cut(s) 978, 1306, 3357
PstNI CAGNNNCTG 3 cut(s) 743, 1079, 3835
PsuI RGATCY 4 cut(s) 2742, 2770, 3389, 3550
PsyI GACNNNGTC 1 cut(s) 797
PvuII CAGCTG 1 cut(s) 4078
RsaI GTAC 7 cut(s) 179, 254, 321, 980, 1148, 1446, 3111
RsaNI GTAC 7 cut(s) 178, 253, 320, 979, 1147, 1445, 3110
RseI CAYNNNNRTG 6 cut(s) 438, 551, 1565, 2237, 3648, 4017
SacI GAGCTC 5 cut(s) 201, 1243, 2141, 3765, 4134
SapI GCTCTTC 2 cut(s) 361, 1314
Sau96I GGNCC 6 cut(s) 362, 448, 2185, 2632, 3412, 3658
SchI GAGTC 7 cut(s) 32, 497, 503, 700, 1600, 1979, 4058
SduI GDGCHC 9 cut(s) 201, 1243, 1581, 2141, 2371, 2953, 3102, 3765, 4134
SfaNI GCATC 9 cut(s) 97, 136, 430, 850, 952, 1030, 1366, 3328, 3652
SfcI CTRYAG 6 cut(s) 974, 1302, 1619, 2879, 2954, 3353
SinI GGWCC 2 cut(s) 2185, 3658
SmiMI CAYNNNNRTG 6 cut(s) 438, 551, 1565, 2237, 3648, 4017
SmlI CTYRAG 7 cut(s) 695, 1094, 1160, 1242, 1368, 3398, 4351
SmoI CTYRAG 7 cut(s) 695, 1094, 1160, 1242, 1368, 3398, 4351
SspI AATATT 4 cut(s) 924, 1259, 2522, 3288
SspMI CTAG 8 cut(s) 770, 854, 1115, 2714, 2838, 3473, 3863, 4262
SstI GAGCTC 5 cut(s) 201, 1243, 2141, 3765, 4134
StyI CCWWGG 4 cut(s) 3369, 3690, 3756, 4059
TaiI ACGT 3 cut(s) 372, 727, 2820
TatI WGTACW 4 cut(s) 177, 978, 1146, 3109
TauI GCSGC 1 cut(s) 4454
TscAI CASTG 6 cut(s) 1287, 1328, 1671, 1795, 2148, 3102
TseFI GTSAC 2 cut(s) 56, 3259
TseI GCWGC 9 cut(s) 274, 728, 1301, 1304, 1897, 3010, 3524, 4405, 4448
Tsp45I GTSAC 2 cut(s) 56, 3259
TspGWI ACGGA 4 cut(s) 3070, 3164, 3999, 4476
TspRI CASTG 6 cut(s) 1287, 1328, 1671, 1795, 2148, 3102
Tth111I GACNNNGTC 1 cut(s) 797
Van91I CCANNNNNTGG 1 cut(s) 3667
Vha464I CTTAAG 2 cut(s) 3398, 4351
VpaK11BI GGWCC 2 cut(s) 2185, 3658
VspI ATTAAT 1 cut(s) 3573
XagI CCTNNNNNAGG 5 cut(s) 2534, 2669, 3419, 3587, 3944
XapI RAATTY 9 cut(s) 619, 1356, 1777, 2699, 2785, 3328, 3364, 3957, 4089
XbaI TCTAGA 2 cut(s) 769, 4261
XmnI GAANNNNTTC 2 cut(s) 3736, 4105
XspI CTAG 8 cut(s) 770, 854, 1115, 2714, 2838, 3473, 3863, 4262
ZraI GACGTC 1 cut(s) 370
Zsp2I ATGCAT 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.