MD16G1197900.v1.1

KIP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006118
Physical Location & Seq
Forward (+)
17755690 .. 17764137
8448 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1197900.v1.1.491

Sequence Viewer

Length: 5946 bp
ATGGCAACCATGTTACACTCCGAGTCCAGACGCTTATATTCTTGGTGGTGGGATAGTCACATTCCAAAGAATTCAAAGTGGCTTCAGGAGAATCTTACAGACATGGATTCCAAAGTCAAAGCAATGATCAAGCTCATTGAAGAAGATGCAGATTCTTTTGCAAGGAGGGCAGAAATGTACTACAAGAAACGCCCGGAGCTCATGAAACTGGTGGAGGAGTTCTATCGAGCATATCGTGCATTAGCAGAGAGGTACGATCATGCAACTGTAGAGCTGCGACACGCTCATCGAACCATGGCAGAAGCATTTCCCAACCAAGTACCTTATGTATTGGCTGATGAATCGCCCTCAGGCTCTTCTGGCCCAGATGTTGGACCTCATACACCAGAAATGCCACATCCAGTTCGTTCCTTATTTAACCCTGATGACTTGCATAAGGATACATTGGGTCTCTCGTCAACCAATTTACAAGCTTTGAAAAGGAATGGAGGAAACTCAGCAGACTCCAACTCTGGAATAAGCAGAAGGGGTCTAAAACAGTTCACCGAGATGTTCACTCCAGGAGAAGTGCCAAATAGCTCAAAGGGTGCAGTAGAGGGAAGGATGAGAGAGGGCTTAAATTTTCATGAGGAAGAAGATATCAAACATCAGTTCCAAAATGGGTACTTTCAATTAACTAGTGAGAACCAAAGTCTCAAGACCCAGGTTCTTTCTCAATCTGAGCGTGCTGCAAAAGCAGAAACTGAAGTTCAAGCCTTAAAGAAAACTCTAGATGAGATACAATCTGAAAAAGACGGAGTCCTTCTTCAGTACGAGCAGAGTTTGGAGAAGTTGTCTACACTGGGGAGGGAACTAGATGATGCACAAACGGCTGTTGGAGGGCTCGATGAACGAGCAAGCAAAGCTGATATTGAAACTAAAATATTGAAGGAAGCCCTTGTGGAGTTAGAAGCAGAGAGGGATGCTGGTCTTCTTCAGTACAATCATTGTTTGGAAAGGATATCTAGCCTGGAGACCATGTTATCATTTTCTCAAAGGGATGCAAAAGGACTTAACGAGCGGGCTGTTAAAGCAGAAACTGAAGCTCAAAAGCTCAAGCAAGAACTTTCAAAACTACAGGCTGAAAAGGAAGATTTTTTTCTTCAGTACAAGCAATGCCTTGAGAAGATATCTGCTTTGGAGACTAAAATCTCAGTTTCTGAGGAAAATGTGAGAATGCTTAATGAACAAATTGAAAGAGCTGAAGGCGAAGTCAAAACTCTGAAAGAATCTCTGGCTATACTGATGGAAGAGAAAGAAGCTGCAGCTCTTCAATACGAGCGGTGCATGGATACAATAGCTAAAATGGAAAGTGAAAATTCTCAGGCTCAAGCAGATGCCAAACGACTAAACAGTGAAGTTTTGACAGGGGCTGCAAAATTGAAGAGTGCCGAAGAACAATGTGATCTCTTGGAGAGATCAAATCACTCTCTGCGGTTAGAGGCGGATGGTCTGTTGAAGAAGATTACAAGTAAAGATCAAGAACTTTCAGAGAAGAATGACCAGATGGAGAAACTTCAGATTCTGATGCAAGAGGAGCACTTACAGTTTGTGCAAGCTGAAGCCACTCTCCACGCTCTTCAGAAGTTGCATTGTCAATCCCAAGAGGATCAGAAAGCTTTAGCTCTGGAGTTCAAAAATGGGCTTCAAATGTTGAAGGACTTGGAGATACGCAAAAATGGTATGGAGGATGATGCCCAACGGGTTAAGGAGGAAAACAAGAGCCTAAGTGAATTGAATCTCTCTTGCACTGTGTCAATAAAGAATCTGCAAGATGAAATCTTCAACATCAAAGAGATGAAAGAGAAACTTGAACAGGAGGTTGCGGTAAAATCAGACCAAAGCAATGCCCTCCAGCAACATATTTTACATTTGGAGGAGGAAATTAAGGGATTGAATAGAAGATATCAAGCTATGGTGAAGCAGGTGGAGTCAGCAGGATTAAATCCTGAATGCTTTGAATCATCTGTGAAAGACTTGCAAAGTGAAAAATCGAAGCTAGAAGATATATGCACAGGGGAGAAAGAACAAAGAGAACTTCTTTATGAGAAGTTGAAGGATATGGGTAAACTTTCAAAGGAGAATGCTATTTTGGAGAGCTCGCTGTTGGGGTTGAACACTGAGTTGGAGGGTTTGAGAGAGGCTGTCAAACAATTGCAAGCGTCTTGCCAGTTTCTTCAGGGAGAAAAATCCACTCTTGTTGCTGAGAAAGCTCTCCTGCTTTCACAGTTACAAATTATCACTCAGAATATGCAGACGCTTTTTGAGAGGAACACCTTGCTGGACAATTCCCTCTCTGTTGCAAACATTGAGCTTGAACGGTTCAGAGCAAGATCGAACAGCTTAGAAGAGTTGTGCCAGTCTCTCAATAATGAGAAATCCAATCTTCTTAATGAGAGAGGCACCCTGGTATTTCAGTTAAAAGATGTTGAAGAGAGACTGCGAAACCTGGAAAAGCGGTTTACAAAATTGGAGAAGAAATATTCTAATTTGGAGAAGGAGAAAGGATCCACACTCAATGCACTCGAAGAACTACAGGGTTCCCTCCTTGCAGAAAAACGAGAGCGTGCAAGTTATATACGTTCAAGTGAGGCCCGATTCGCAGGTTTGGAAAACAATGTCCATCTGATGCAGGAAGAAAGAAAGCTGGGAAAGAAAGATTTTGAAGAAGAACTAGATAAAGCTTTAAATGCCCAGATCGAGATCTTTATCCTTCAGAAGTTTATAGAAGATCTGGAAGAGAAGAATTTGTCCTTATTCATCGAGTGTCAGAGACACGTTGAGAAATCCAAGTTTTCTAGTAAGCTGATCTCAGAGTTGGAGAATGAAAATCTTGAACTACAGGTGGAAGAACAATTCTTGGTGGAAGAAATCGACAAGTTGAGGTTGGGAATTCGTCAAGTGCTCAGGGCTCTTGAGGTTGAACCAGATAGGCACGACGATAAGACTGAACCAGGGAAAGTAAATATGCCGCACGTTTTGAATACTATCAAGGACTTGAAAACTTCTTTGTTACGTAGCAAGGATGAGGAACAGCAGTTGCTTGTTGAGAAGTCAGTGCTCTTAAGTGTACTTGGGCAGATGAGATTAGAGGGTGCAGAGACAGAGACGGAAAAGCAATTCTTTGAGGGGGAGTATGAGATCATGATAAACCATTGTTCTATGCTACAAAAAGAGAAGCATGACCTTCTAGAGATGACAAGGGAGTTGAGGTTGGAAGTGACCGAGAAAGAGCACAAGGAGGAAATATTAGAGGCTCAACTGGTAACTCTTCAACGTAAGCTGGCAAATTTGGAGAACAATTACGTGGTTTTGCAAGAAGAAAATTACAAGGTGCTTGAAGATAAGAGATCTTTGCTTAAGGATCTTTTGGAACTCAAAGAGGAAAATCAAATGCTTGAAGAGGATAATATTGTTAATTTCCATGAAGCACTAGCTTTCAGCACCCTCTCATTGGTTTTAGAGAGCTTCGCAACCGAGAAAGCTGCAGAACTAAAAGCACTTACTGAAGATCTCAACAGTCAATTTGTAATTAACAACGACCTCAAAGAGGCTGTTGGGATATTGGAGGAGCAGTTAGTGATGAAAGAAGCAGAAAATCTGCATCTGAGCGAGACAGTCGAATTGTTGGACAAGGAACTGTGTGACTCCAAGGACTTAAATGGTCAACTAAGCCATCAGATCTCAGTTGGAAACGATTCTCTGAAACAGAAAACCATGAAGCTCTTGGACGCAGAAGAGAAGCTTAAAAGGACAGAGGACTTGAATGTGGAATTGTGCAGAAGAGTTCAGGAACTGAAGATGGATATTGAAGAGTCAAAACTAATGCAACAAAATTGCAAGAAGCAGATTCTTGAACTATCTGAAGATAGCACAAGCCAGAAAAAGGAAATCAATAGCCTTTGTGAAGCAAATGAAATTCTGAAGAATGAAATCTTGTCTAACGTAATTGAAAAAGAAGTAGAAAATCTACATCTGAACGAGACAGCTCAATTGTTGCACGAGGAACTGCGTGAAGTCAAGGACTCGAAAGATCAGCTAAACCATCAAATTTTAGCTGGAAAGGATTCTCTGAAGCAGAAAACCATGGAGCTCTCAGAAGTAGAAGAGAAGCTTAGAAAGGCCGAGGACTTGAATGTGGAATTATGCAGAACTGTTCAGGAGCTGATGATGGAACATGAAGATTCAAATCTTCTGAGAGAAAATTGTGAGAGGCAGATTCTAGAACTATCCAAAGATAACTCGAATCAGAAAAATGAAATTGAGATCCTTCGTGAAGCAAATGAAACTCTGGAGAAGGAAGTGGGTATGTTAAGTGAAGTAATTGAAGAATATAGAAGTAGAGAAGAGTATCTGAGTTCCGAGCTACAAGAGAGAAGCAATGACTTTGAACTCTGGGAGGCTGAGGCTGCGACATTCTTCTTTGATCTTCAAGTTTCTGCTGTCCGTGAAGCTTTTCTTGAAAATACGGTTCATGAGCTCACTGATTTTTGTGAGAGTCTTAAAGATGAAGGTGCTGCAAAATATGTGGAGGTCAAACAAATGAAAGAAAGTGTTAGCTCCTTGGAAGGTGAAGTTGGGGAGCTGATGGGCCAGTTGTCTGCATATGTACCTGTCGTAGCCTCATTGAGAGAGAATGTAGCATCTCTTCAGCACAATACTGTACTTAGATCGAAGCTTCTTGTGGAAAGAAATCAACAATATAAGGGTGTGGAACCGCCAAATCATCTACATGAAAATAGCTGTCAACATTCCACAGCATCGGTACCACACGGAATTTCAGAATTGGCAGAAATGCAAACAATGATCAAAGAAGTTGAAAAGATGTTTGTCGAGGAAACGGAAAGGCTTGTGATGGAACCATTTGAAAAGGCAATGGTGGAAGAAATTGAAAGGCTTTCAACTCAGGAAAGTACAAAAAACAGCAACGTCTCTGTGGAGATTGAAGAGTTACAATCAAACGGTACCTCACTTCAAGAAAAATGCAGTAAAAGTGAAGAGATGAAACTTGGAAAGGAGTTCACTGGTGAAAATCTCAAGTTGCTGAAGACAAAATCTGATAATGGGATTTGGATGAAAGACATTCCGCTTGATCATGTTTCTGATTGTTCATCCCATGGAAAAAGCAGGAGAGGTACTGGTGGGGCTGATAATCAGATGCTTGAGTTATGGGAAACTGGCGAACAATACAGTCACCAGGATCCTGTTCCCAATGAGAAACAAAATCAGGCATCTGCACGCATGGAAGATCTTACCCCTAGCCATCGGTTTACAGATTCTGAGCCGATGATTCAGAATTTTACTTCAGAAGTTCAGGCTGAGAAGGAGTTGGGAATTGATAAGCGGGAAGTGTCATTTCGAAGGCCACATCATGAAAGCAAGAAGGAAAAAATCCTGGAGAGACTTGCTTCTGATGCGCAGAAACTGACGAGTCTGCAAACGATTGCTCGGGATTTGGATAAGAAGATGGAGACAAGCAAGAAGGGTAAAAGGGCCAACGGCATTGAATATGAAACAGTCAAAAGACACTTGGTTGAAGTTGAGGAGGCAGTTGTGCAGCTAGTGGAAACTAATGATCAACTGAAAAAGAACATTGAGGAGTCTCCGTCTTTGGACGAGCAGACTTCCATAGAGTTGGAGGAGGCTGGAAATGTCCGCAGAGAGAGAGTAGTGAAAGAGGCAAGTAAAGGGTCTGAGAAGATCGGAAGGCTGCAGTTTGAGTTGCAGAACATCCACTACATTTTGCTGAAACTGGAGGACGAGAATAAGAAGAAAGGGAGGCATGGATTTTACGTAAGCAGAACAGGCGTTCTCCTGAGGGATTTTATTTACAGCGGAAGAAATAGCGAGAGGCGCAAGAAGGGTTGTGTGTGTGGCTGCATGAGACCGTCCACGAATGGAGAATGA

Protein Analysis

1982

Amino Acids

227.49

Weight (kDa)

5.03

Isoelectric Point (pI)

51.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 14 - 86 1.1e-36 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1954
Acc16I TGCGCA 1 cut(s) 5457
Acc36I ACCTGC 2 cut(s) 1954, 2633
Acc65I GGTACC 2 cut(s) 4804, 5003
AccB1I GGYRCC 3 cut(s) 2441, 4804, 5003
AccB7I CCANNNNNTGG 1 cut(s) 371
AccBSI CCGCTC 2 cut(s) 1060, 1321
AccI GTMKAC 1 cut(s) 836
AclWI GGATC 7 cut(s) 1656, 2541, 2554, 3411, 4300, 5234, 5247
AfiI CCNNNNNNNGG 4 cut(s) 371, 3493, 3589, 3925
AflII CTTAAG 2 cut(s) 3103, 3398
AflIII ACRYGT 1 cut(s) 2815
AhlI ACTAGT 1 cut(s) 677
AjnI CCWGG 8 cut(s) 559, 702, 1008, 2445, 2487, 2992, 5235, 5433
AjuI GAANNNNNNNTTGG 6 cut(s) 2114, 2146, 2147, 2179, 4599, 4631
Alw21I GWGCWC 8 cut(s) 201, 1581, 2141, 2946, 3102, 3276, 4134, 4521
AlwI GGATC 7 cut(s) 1656, 2541, 2554, 3411, 4300, 5234, 5247
AlwNI CAGNNNCTG 9 cut(s) 743, 1079, 1199, 1412, 1564, 3835, 4204, 5318, 5464
Ama87I CYCGRG 1 cut(s) 5487
AoxI GGCC 6 cut(s) 361, 2631, 4161, 4630, 5402, 5532
Asp700I GAANNNNTTC 4 cut(s) 306, 3736, 4105, 4494
Asp718I GGTACC 2 cut(s) 4804, 5003
AspLEI GCGC 2 cut(s) 5458, 5895
AspS9I GGNCC 5 cut(s) 362, 374, 2632, 4630, 5532
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 5 cut(s) 535, 1969, 4622, 5078, 5225
AsuII TTCGAA 1 cut(s) 5398
AvaI CYCGRG 1 cut(s) 5487
AvaII GGWCC 1 cut(s) 374
AxyI CCTNAGG 2 cut(s) 349, 5855
BamHI GGATCC 2 cut(s) 2546, 5239
BanI GGYRCC 3 cut(s) 2441, 4804, 5003
BanII GRGCYC 6 cut(s) 201, 885, 2141, 2953, 4134, 4521
BarI GAAGNNNNNNTAC 2 cut(s) 4671, 4703
BauI CACGAG 1 cut(s) 4040
BbsI GAAGAC 2 cut(s) 962, 5093
Bbv12I GWGCWC 8 cut(s) 201, 1581, 2141, 2946, 3102, 3276, 4134, 4521
BbvCI CCTCAGC 1 cut(s) 4443
BceAI ACGGC 2 cut(s) 885, 5554
BcgI CGANNNNNNTGC 2 cut(s) 3506, 3540
BciT130I CCWGG 8 cut(s) 561, 704, 1010, 2447, 2489, 2994, 5237, 5435
BciVI GTATCC 2 cut(s) 433, 1324
BclI TGATCA 4 cut(s) 126, 4845, 5131, 5614
BcnI CCSGG 1 cut(s) 194
BcuI ACTAGT 1 cut(s) 677
BfmI CTRYAG 7 cut(s) 267, 1115, 1302, 2573, 2879, 3525, 5750
BfrI CTTAAG 2 cut(s) 3103, 3398
BfuAI ACCTGC 2 cut(s) 1954, 2633
BfuI GTATCC 2 cut(s) 433, 1324
BglII AGATCT 6 cut(s) 2742, 2770, 3389, 3550, 3720, 5287
Bme1390I CCNGG 9 cut(s) 194, 561, 704, 1010, 2447, 2489, 2994, 5237, 5435
Bme18I GGWCC 1 cut(s) 374
BmeT110I CYCGRG 1 cut(s) 5487
BmgT120I GGNCC 5 cut(s) 362, 374, 2632, 4630, 5532
BmiI GGNNCC 8 cut(s) 2443, 2548, 2581, 4755, 4806, 4899, 5005, 5241
BmrFI CCNGG 9 cut(s) 194, 561, 704, 1010, 2447, 2489, 2994, 5237, 5435
BmrI ACTGGG 1 cut(s) 851
BmuI ACTGGG 1 cut(s) 851
BpiI GAAGAC 2 cut(s) 962, 5093
BpmI CTGGAG 7 cut(s) 543, 1031, 1688, 1877, 4352, 5456, 5813
Bpu10I CCTNAGC 2 cut(s) 2945, 4443
Bpu14I TTCGAA 1 cut(s) 5398
BpuEI CTTGAG 7 cut(s) 680, 1079, 1181, 1353, 2975, 5060, 5222
BpuMI CCSGG 1 cut(s) 194
BsaAI YACGTR 3 cut(s) 3056, 3346, 5833
BsaBI GATNNNNATC 3 cut(s) 2741, 2747, 4227
BsaI GGTCTC 3 cut(s) 455, 1007, 5917
BsaJI CCNNGG 9 cut(s) 294, 702, 2445, 2993, 3690, 4125, 4164, 4602, 5155
Bsc4I CCNNNNNNNGG 4 cut(s) 371, 3493, 3589, 3925
Bse21I CCTNAGG 2 cut(s) 349, 5855
Bse3DI GCAATG 5 cut(s) 129, 1160, 1891, 4426, 4920
Bse8I GATNNNNATC 3 cut(s) 2741, 2747, 4227
BseBI CCWGG 8 cut(s) 561, 704, 1010, 2447, 2489, 2994, 5237, 5435
BseDI CCNNGG 9 cut(s) 294, 702, 2445, 2993, 3690, 4125, 4164, 4602, 5155
BseJI GATNNNNATC 3 cut(s) 2741, 2747, 4227
BseLI CCNNNNNNNGG 4 cut(s) 371, 3493, 3589, 3925
BseMI GCAATG 5 cut(s) 129, 1160, 1891, 4426, 4920
BseRI GAGGAG 7 cut(s) 230, 1589, 1931, 3623, 5597, 5651, 5693
BseYI CCCAGC 1 cut(s) 2686
BsgI GTGCAG 5 cut(s) 609, 3156, 3838, 5259, 5615
BshFI GGCC 6 cut(s) 363, 2633, 4163, 4632, 5404, 5534
BshNI GGYRCC 3 cut(s) 2441, 4804, 5003
BsiHKAI GWGCWC 8 cut(s) 201, 1581, 2141, 2946, 3102, 3276, 4134, 4521
BsiHKCI CYCGRG 1 cut(s) 5487
BsiSI CCGG 1 cut(s) 194
BslI CCNNNNNNNGG 4 cut(s) 371, 3493, 3589, 3925
BsmBI CGTCTC 2 cut(s) 3140, 4975
BsmI GAATGC 3 cut(s) 1221, 1997, 2128
BsnI GGCC 6 cut(s) 363, 2633, 4163, 4632, 5404, 5534
Bso31I GGTCTC 3 cut(s) 455, 1007, 5917
BsoBI CYCGRG 1 cut(s) 5487
Bsp119I TTCGAA 1 cut(s) 5398
Bsp19I CCATGG 3 cut(s) 294, 4125, 5155
BspANI GGCC 6 cut(s) 363, 2633, 4163, 4632, 5404, 5534
BspHI TCATGA 5 cut(s) 201, 625, 3183, 4513, 5410
BspLI GGNNCC 8 cut(s) 2443, 2548, 2581, 4755, 4806, 4899, 5005, 5241
BspMAI CTGCAG 3 cut(s) 1306, 3529, 5754
BspMI ACCTGC 2 cut(s) 1954, 2633
BspPI GGATC 7 cut(s) 1656, 2541, 2554, 3411, 4300, 5234, 5247
BspQI GCTCTTC 3 cut(s) 361, 1314, 1623
BspT104I TTCGAA 1 cut(s) 5398
BspT107I GGYRCC 3 cut(s) 2441, 4804, 5003
BspTI CTTAAG 2 cut(s) 3103, 3398
BspTNI GGTCTC 3 cut(s) 455, 1007, 5917
BsrBI CCGCTC 2 cut(s) 1060, 1321
BsrDI GCAATG 5 cut(s) 129, 1160, 1891, 4426, 4920
BssECI CCNNGG 9 cut(s) 294, 702, 2445, 2993, 3690, 4125, 4164, 4602, 5155
BssSI CACGAG 1 cut(s) 4040
BssT1I CCWWGG 5 cut(s) 294, 3690, 4125, 4602, 5155
Bst2BI CACGAG 1 cut(s) 4040
Bst2UI CCWGG 8 cut(s) 561, 704, 1010, 2447, 2489, 2994, 5237, 5435
BstAFI CTTAAG 2 cut(s) 3103, 3398
BstAPI GCANNNNNTGC 1 cut(s) 236
BstBAI YACGTR 3 cut(s) 3056, 3346, 5833
BstBI TTCGAA 1 cut(s) 5398
BstC8I GCNNGC 9 cut(s) 726, 898, 1062, 1596, 2141, 2199, 2607, 3324, 5278
BstDSI CCRYGG 3 cut(s) 294, 4125, 5155
BstENI CCTNNNNNAGG 1 cut(s) 3587
BstHHI GCGC 2 cut(s) 5458, 5895
BstNI CCWGG 8 cut(s) 561, 704, 1010, 2447, 2489, 2994, 5237, 5435
BstSCI CCNGG 9 cut(s) 192, 559, 702, 1008, 2445, 2487, 2992, 5235, 5433
BstSFI CTRYAG 7 cut(s) 267, 1115, 1302, 2573, 2879, 3525, 5750
BstSNI TACGTA 2 cut(s) 3056, 5833
BstV2I GAAGAC 2 cut(s) 962, 5093
BstXI CCANNNNNNTGG 1 cut(s) 5674
Bsu36I CCTNAGG 2 cut(s) 349, 5855
BsuI GTATCC 2 cut(s) 433, 1324
BsuRI GGCC 6 cut(s) 363, 2633, 4163, 4632, 5404, 5534
BtgI CCRYGG 3 cut(s) 294, 4125, 5155
BtsIMutI CAGTG 7 cut(s) 839, 1399, 1788, 2157, 3102, 4521, 5061
BveI ACCTGC 2 cut(s) 1954, 2633
Cac8I GCNNGC 9 cut(s) 726, 898, 1062, 1596, 2141, 2199, 2607, 3324, 5278
CaiI CAGNNNCTG 9 cut(s) 743, 1079, 1199, 1412, 1564, 3835, 4204, 5318, 5464
CciI TCATGA 5 cut(s) 201, 625, 3183, 4513, 5410
CfoI GCGC 2 cut(s) 5458, 5895
Cfr13I GGNCC 5 cut(s) 362, 374, 2632, 4630, 5532
CseI GACGC 4 cut(s) 39, 2190, 2305, 3779
CspCI CAANNNNNGTGG 4 cut(s) 2221, 2256, 4548, 4583
DraI TTTAAA 1 cut(s) 2727
EciI GGCGGA 1 cut(s) 1499
Ecl136II GAGCTC 4 cut(s) 199, 2139, 4132, 4519
Eco105I TACGTA 2 cut(s) 3056, 5833
Eco130I CCWWGG 5 cut(s) 294, 3690, 4125, 4602, 5155
Eco24I GRGCYC 6 cut(s) 201, 885, 2141, 2953, 4134, 4521
Eco31I GGTCTC 3 cut(s) 455, 1007, 5917
Eco32I GATATC 4 cut(s) 640, 1002, 1170, 1946
Eco47I GGWCC 1 cut(s) 374
Eco53kI GAGCTC 4 cut(s) 199, 2139, 4132, 4519
Eco81I CCTNAGG 2 cut(s) 349, 5855
Eco88I CYCGRG 1 cut(s) 5487
EcoICRI GAGCTC 4 cut(s) 199, 2139, 4132, 4519
EcoNI CCTNNNNNAGG 1 cut(s) 3587
EcoRI GAATTC 2 cut(s) 70, 2931
EcoRII CCWGG 8 cut(s) 559, 702, 1008, 2445, 2487, 2992, 5235, 5433
EcoRV GATATC 4 cut(s) 640, 1002, 1170, 1946
EcoT14I CCWWGG 5 cut(s) 294, 3690, 4125, 4602, 5155
EcoT38I GRGCYC 6 cut(s) 201, 885, 2141, 2953, 4134, 4521
ErhI CCWWGG 5 cut(s) 294, 3690, 4125, 4602, 5155
Esp3I CGTCTC 2 cut(s) 3140, 4975
FauI CCCGC 2 cut(s) 1053, 5376
FauNDI CATATG 1 cut(s) 4645
FbaI TGATCA 4 cut(s) 126, 4845, 5131, 5614
FblI GTMKAC 1 cut(s) 836
FriOI GRGCYC 6 cut(s) 201, 885, 2141, 2953, 4134, 4521
FspI TGCGCA 1 cut(s) 5457
GlaI GCGC 2 cut(s) 5457, 5894
GsaI CCCAGC 1 cut(s) 2690
GsuI CTGGAG 7 cut(s) 543, 1031, 1688, 1877, 4352, 5456, 5813
HaeIII GGCC 6 cut(s) 363, 2633, 4163, 4632, 5404, 5534
HapII CCGG 1 cut(s) 194
HgaI GACGC 4 cut(s) 39, 2190, 2305, 3779
HhaI GCGC 2 cut(s) 5458, 5895
Hin6I GCGC 2 cut(s) 5456, 5893
HinP1I GCGC 2 cut(s) 5456, 5893
HincII GTYRAC 3 cut(s) 459, 3707, 4787
HindII GTYRAC 3 cut(s) 459, 3707, 4787
HindIII AAGCTT 7 cut(s) 471, 1656, 2721, 3782, 4151, 4491, 4715
HpaII CCGG 1 cut(s) 194
HphI GGTGA 5 cut(s) 535, 1969, 4622, 5078, 5225
Hpy99I CGWCG 1 cut(s) 2981
HpyCH4IV ACGT 9 cut(s) 2620, 2817, 3015, 3055, 3316, 3345, 3984, 4968, 5832
HpySE526I ACGT 9 cut(s) 2620, 2817, 3015, 3055, 3316, 3345, 3984, 4968, 5832
HspAI GCGC 2 cut(s) 5456, 5893
KpnI GGTACC 2 cut(s) 4808, 5007
Ksp22I TGATCA 4 cut(s) 126, 4845, 5131, 5614
LguI GCTCTTC 3 cut(s) 361, 1314, 1623
LmnI GCTCC 7 cut(s) 196, 1576, 3610, 4129, 4201, 4604, 4621
MaeII ACGT 9 cut(s) 2620, 2817, 3015, 3055, 3316, 3345, 3984, 4968, 5832
MaeIII GTNAC 9 cut(s) 12, 56, 2268, 3051, 3259, 3304, 3683, 4989, 5231
MbiI CCGCTC 2 cut(s) 1060, 1321
MfeI CAATTG 2 cut(s) 2192, 4031
MmeI TCCRAC 9 cut(s) 352, 531, 856, 2145, 2838, 3234, 3648, 3709, 5655
MroXI GAANNNNTTC 4 cut(s) 306, 3736, 4105, 4494
MslI CAYNNNNRTG 2 cut(s) 548, 4770
MspA1I CMGCKG 1 cut(s) 5874
MspCI CTTAAG 2 cut(s) 3103, 3398
MspI CCGG 1 cut(s) 194
MspR9I CCNGG 9 cut(s) 194, 561, 704, 1010, 2447, 2489, 2994, 5237, 5435
MunI CAATTG 2 cut(s) 2192, 4031
Mva1269I GAATGC 3 cut(s) 1221, 1997, 2128
MvaI CCWGG 8 cut(s) 561, 704, 1010, 2447, 2489, 2994, 5237, 5435
NciI CCSGG 1 cut(s) 194
NcoI CCATGG 3 cut(s) 294, 4125, 5155
NdeI CATATG 1 cut(s) 4645
NlaIV GGNNCC 8 cut(s) 2443, 2548, 2581, 4755, 4806, 4899, 5005, 5241
NmeAIII GCCGAG 1 cut(s) 4189
NmuCI GTSAC 4 cut(s) 56, 3259, 3683, 5231
NsbI TGCGCA 1 cut(s) 5457
NspV TTCGAA 1 cut(s) 5398
PagI TCATGA 5 cut(s) 201, 625, 3183, 4513, 5410
PaqCI CACCTGC 1 cut(s) 1954
PciSI GCTCTTC 3 cut(s) 361, 1314, 1623
PcsI WCGNNNNNNNCGW 1 cut(s) 232
PctI GAATGC 3 cut(s) 1221, 1997, 2128
PdmI GAANNNNTTC 4 cut(s) 306, 3736, 4105, 4494
PflFI GACNNNGTC 1 cut(s) 797
PflMI CCANNNNNTGG 1 cut(s) 371
PfoI TCCNGGA 2 cut(s) 559, 5433
Ppu21I YACGTR 3 cut(s) 3056, 3346, 5833
Psp124BI GAGCTC 4 cut(s) 201, 2141, 4134, 4521
Psp6I CCWGG 8 cut(s) 559, 702, 1008, 2445, 2487, 2992, 5235, 5433
PspFI CCCAGC 1 cut(s) 2686
PspGI CCWGG 8 cut(s) 559, 702, 1008, 2445, 2487, 2992, 5235, 5433
PspN4I GGNNCC 8 cut(s) 2443, 2548, 2581, 4755, 4806, 4899, 5005, 5241
PspPI GGNCC 5 cut(s) 362, 374, 2632, 4630, 5532
PsrI GAACNNNNNNTAC 2 cut(s) 5759, 5791
PstI CTGCAG 3 cut(s) 1306, 3529, 5754
PstNI CAGNNNCTG 9 cut(s) 743, 1079, 1199, 1412, 1564, 3835, 4204, 5318, 5464
PsyI GACNNNGTC 1 cut(s) 797
RseI CAYNNNNRTG 2 cut(s) 548, 4770
SacI GAGCTC 4 cut(s) 201, 2141, 4134, 4521
SapI GCTCTTC 3 cut(s) 361, 1314, 1623
Sau96I GGNCC 5 cut(s) 362, 374, 2632, 4630, 5532
ScrFI CCNGG 9 cut(s) 194, 561, 704, 1010, 2447, 2489, 2994, 5237, 5435
SfcI CTRYAG 7 cut(s) 267, 1115, 1302, 2573, 2879, 3525, 5750
SfuI TTCGAA 1 cut(s) 5398
SinI GGWCC 1 cut(s) 374
SmiMI CAYNNNNRTG 2 cut(s) 548, 4770
SmlI CTYRAG 9 cut(s) 695, 1094, 1160, 1368, 2954, 3103, 3398, 5075, 5201
SmoI CTYRAG 9 cut(s) 695, 1094, 1160, 1368, 2954, 3103, 3398, 5075, 5201
SnaBI TACGTA 2 cut(s) 3056, 5833
SpeI ACTAGT 1 cut(s) 677
SspI AATATT 4 cut(s) 924, 2522, 3288, 3451
SstI GAGCTC 4 cut(s) 201, 2141, 4134, 4521
StyD4I CCNGG 9 cut(s) 192, 559, 702, 1008, 2445, 2487, 2992, 5235, 5433
StyI CCWWGG 5 cut(s) 294, 3690, 4125, 4602, 5155
TaiI ACGT 9 cut(s) 2623, 2820, 3018, 3058, 3319, 3348, 3987, 4971, 5835
TaqII GACCGA 1 cut(s) 3278
TatI WGTACW 6 cut(s) 177, 978, 1146, 3109, 4702, 4952
TauI GCSGC 1 cut(s) 3013
TscAI CASTG 7 cut(s) 846, 1399, 1795, 2164, 3102, 4528, 5068
TseFI GTSAC 4 cut(s) 56, 3259, 3683, 5231
Tsp45I GTSAC 4 cut(s) 56, 3259, 3683, 5231
TspGWI ACGGA 6 cut(s) 810, 3164, 4475, 4827, 4895, 5634
TspRI CASTG 7 cut(s) 846, 1399, 1795, 2164, 3102, 4528, 5068
Tth111I GACNNNGTC 1 cut(s) 797
Van91I CCANNNNNTGG 1 cut(s) 371
Vha464I CTTAAG 2 cut(s) 3103, 3398
VpaK11BI GGWCC 1 cut(s) 374
XagI CCTNNNNNAGG 1 cut(s) 3587
XbaI TCTAGA 3 cut(s) 769, 3229, 4261
XcmI CCANNNNNNNNNTGG 1 cut(s) 3763
XmiI GTMKAC 1 cut(s) 836
XmnI GAANNNNTTC 4 cut(s) 306, 3736, 4105, 4494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.