Rroxscaffold_5G00334110

KIP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006118
Physical Location & Seq
Forward (+)
1435140 .. 1443431
8292 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00334110.1

Sequence Viewer

Length: 6387 bp
ATGGCAACCCTGTTACAGTCTGAGTCCAGACGCTTGTATTCATGGTGGTGGGACAGTCACATTAGCCCAAAGAACTCAAAATGGCTTCAGGAAAATCTTACAGATATGGATGCTAAAGTCAAAGCAATGATCAAGCTCATTGAAGAAGATGCAGACTCTTTTGCAAGGAGAGCAGAAATGTACTACAAGAAACGCCCCGAGCTCATGAAACTGGTTGAGGAGTTCTATAGAGCTTATCGTGCATTGGCTGAGAGGTATGATCATGCAACTGTGGAGCTGCGCCAGGCTCATCGAACCATGGCAGAAGCATTTCCCAACCAAGTACCTTATGAACTCGCCGATGAATCATCCTCAGGCTCTTATGGCCCAGAGGCTGGACCTCATACACCAGAAATGCTGCATCCAGTTCGTGCCTTATTTGACTCAGATGACTTGCATAAGGATGCACTGGGAGTGGGACTCTCTTCAACTGATTTACATGATTTGAAAAGGAACGGAGGCTCCGACTCTGGAATAAGCAAAAGAGGTCTGAAGCAGCTTAAGGACATGTTCAATCCAGAAGAAGAGTGTAATCCAAAGGTTGCGGGGGGAAGGTTTACAGAAGGCTTAAGTTTTCATGAATCAGATGAAAGCAAACCAAAGTTACAGAATGGGTACTCTCAGTTAACTAGTGAGAACCAGAGTCTCAAGAACCAGGTCCTCTCTCAATCTGAGCGTGCTGCAAAAGCTGAAACTGAAATCCAAATCCTACAGAAAACTCTATCTGAGATACAAGCTGAAAAGGACACAGTCCATCATCAGTACCAGCAGAGTTTGGAGAAGTTATCTAAGCTGGGGAAAGAACTGAATAATGCTCAAGAGGCTGCTGGAGGGCTCAATGAACGAGCCAGTAAAGCTGATATTGAAATTACAATACTGAAGGAAGCCCTTGGGGAATTAGAAGCTGAGAGAGATGCTGGTCTTCATCAGTACAATCGGTGTCTGGAAAGGATATCTAGCCTGGAGACCATGTTATCTTTTTCCCGGGAGGATGCAAAAGGACTTAATGAGCGAGCTGTTAAAGCAGAAACTGAAGCTCAAAATATGAAGCAAGAACTTTCTAAATTAGAGGCAGAAAAGGAAGCCTGTTTTCTCAGGTACAAGCAATGTCTTGAGAAGATATCTGCTCTGGAGGCTACGATCTCAGTTGATGAAGAAAATGCCAGAATCCTTAATGAACAAATTGAAAGAGCTGAAAGTGAAGTTAAATCACTGAACGAAGTTGTTGCTATACTGAAGGAAGAGAAAGAATCTGCATCTCTACAATTCAAGCAGTACATGGATACAATAGCTAAGATGGAAAGAGAACTTTCTCAAGCTCAAGCGGATGCTGAAAGATTGAATAGTGAAATTTTGAGAGGGGCAGCAAAATTAAAGGGTGCCGAAGAACAATGTGTTCTCTTGGAGAGATCAAATCAGTCTCTGCGGTTAGAGGCAGATGGTCTGGTCAAGAAGATTGCAACAAAAGATGAAGAACTTTCAGACAAGAATGATGAGATGGAGAAACTTCATAATCTGATGCAAGATGAGCACTTGCGGTTTGTGCAAGCTGAAGCCACTCTCCAATTTCTGCAGAAGTTGCATTCTCAATCCCAAGAAGAGCAGAAAGCTTTAGCCCTGGAATTCAAAAACGGGCTTCAGATGTTGAAGGACTTGGAGTTGAGCAAACATGGTGTGGAGGATGATATGCAACGGGTTAAGGAAGAAAATAAGAGCTTGAATGAACTGAACTTCTCTTGTACTATCTCCATTAAGAACCTGCAAGATGAAATCTTTAGCATGAAGGAGATGAAAGAGAAACTTGAAGAGGAGGTTAAGCTTAAAACAGACCAGAGCAATGCTCTCCAGAGGCAAATTTCCCATTTGGAGGATGAAATTAAGGGGTTGAATGGAAGATATCAGGCTATTGTGGAGCAAGTGGAGTCAGTAGGTTTAACTCCTGAATGCTTTGGATCATCTGTGAAAGACTTGCAAAATGAGAAATCAAGGCTAGAAGATATATGCACAAGGGATAGAGAAGACAGAGAACATCTTTACGAGAAGTTGAAGGATATGGGTAAACTTTCAAAGGAGAATGCTGTTCTGGAGAGTTCACTTTTGGGTTTGAATGGGGAGTTAGAGGGTTTGAGAGGGAATGTCAAGGAATTGCAGGAGTCTTGCCACTTCCTCCAGGGAGAAAAAGCCACTCTTGTTGCTGAGAAATTTGCCCTGCTTTCCCAGTTGCAAATTATCACTCAGAACATGCACAAGCTTTTCGAGAAGAACTCATTGCTGGAAAGTTCCCTCTCCGGAGCAAACATTGAGCTCGAAAAGTTGAGGGCAAGAGCAAAGAGCTTAGAAGAGCTGTGCCAGTTACTCAATAACGAGAAGTCCAATCTTCTTAATGAGAGAGGCACGCTGGTGTTTCAGTTAAATGATGTTGAGGAGAGACTGCGAAGCCTGGAAAAGAGGTTTAAAAAATTAGAGAAGAAGTACTCTAAAATGGAGAAGGAGAAAGAGTCCACACTTGATGCAGTAGAAGAATTACGGGGTTCCTTGCATGCAGAAAAACGAGAGCGTGCTAGTTATATTCGGTCAAGTGAATCCCGATTGGCAGGTTTGGAAAGCAATGTCCATCTCCTGCAGGAAGAAAGAAGGTTGGGAAAGAAAGAATTTGAAGCAGAACTTGACAAAGCTGTGAATGCCCAGATTGAGATCTTTATCCTTCAGAAGTTTATACAAGATCTGGAAGAGAAGAATTTGACAATATACATTGAATGTCAGAGACATGTTGAGGCTTCCAAGTTTTCTGATAAGCTGATCAAAGAGTTGGAGAGTGAAAATCTTGAGCTACAGGTGGAAGAAGAATTCTTAGTAGAAGAAATTGAAAAGTTGAGGCTGGGAATTTATCAAGTGTTCAGGTCTCTGCATATCGAAACAGGTACACATGAAGATAAGATTGAGCGAGAAAAAGTACTTGTGCTGCACATTTTGAATGCTATCAAGGACTTGAAAAGTTCTCTGGTTCGGAGCAAAGATGAGGAGCAGCAGTTGCTGGTTGAGAATTCAGTGCTCTTTACTTTACTTCGGGAGCTGAGATCCGAAGGTGCAGAGATAGAGTCGGCAAAACAAACTTTGGAGCAGGAGTATGAGGTAATGGCAGACCATTGTGCTATGCTACAAAATGACAAGCATGAGCTTCTAGAGACGCAAAGGCAGTTGAGGTTGGAAGTGACTGAGAAAGAGCAAAAGGAGGAAATATTAGAGGCTGATTTGGAAAGTCTTCAAGGTAAGCTGGTGAGTCTGCAAGACGCATACCTGATTTTGCAGGAAGAACAATCCAAGGTGCTTGAAGAGAGGAGATCCTTGCTTAAGAAGCTTTTGGACCTGAAAGAGGAAAAGCAAGTCCTTGAAGAGGATAATAATGTTAATTTTCATGAAGCACTGGCTTTCAGCAACGTCTCTATGGTTTTAGAGAGCTTCACAATTGAGAAAGCTGTGGAACTAAAAGCACTTGCTGATGATCTTGATGGTCTTTGTGCTACTAACAATGACCTTAAAGATGCGGTTCGAACGTTGGGTGAGAAGTTAGTGATGAAAGAAGTAGAGAATCTACATCTGAATGAGATGGTTCAATTGTTGGACAAGGAACTGTGTGAAGCCAGTGACTTAAATGGTTTACTAAGCCATCAAATTTCAATTGGAAAGGATTCTCTCAAACAGAAAAACATGAAGCTCTCAGAGGTAGAAGAGAAGCTTCAAAAGACAGAGGACTTGAATGTGGAATTATGCAGAACTGTTGAGGAGCTGAAGATGGAATATGAAGAGTCAAAAATAATGGGTCAAAATTATGAGAAGCAGATTCTCCAACTATCTGAAGTCAGCACTATTCAGAAAAAGGAAATGAATAGCCTGCGTGAAGCAAATGAAACTCTGGAGGATGAAATCTTATGTAAATCGATTGAAAAAGAAATAGAAACTCTGCACCTGAGTGAGACAGCCCAATTGTTGGACATGGAATTGTGTGAAGCCAAGGACTCAAATGATCAACTGAGCCATAGAATCTTAGTTGGAGAAGGTTGTCTGAAACAGAAAACTACGGAGCTTTCAGAAGCAGAAGAGAAGCTTAGACAGACAGAAGACTTGAATGTTGAACTATGCAGAAATGTTCAGGAACTGAAGGTGCAACATGAAGAATCAAAACTCATAAGAGAAAATTGTGAGAAGGAGATTGTGGAGCTATCCGAAGATAAAACAAATCAGAAAATGGAAATTGAGAGCCTCCGTGAAGCAAATGGAACTCTAGAGATTGAAGTGGGTGTCTTGTGTGAAGCAATTGAAGAATACAAAATTAGAGAAGACTATATGAGTTCAGAGCTGCAAGAAAGAAGCAATGAGTTTGAACTCTGGGAGGCTGAGGCTACAACATTCTACTTTGATCTTCACAATTCTGCTGTCCGTGAAGTTTTTCTTGAAGATAAGGTTCATGAGCTTACAGAAGTCTGTGAGAGAATTAAGGATGAAGATGCTGCAAAATTTGTGGAGATTGAACAAATGAAAGAAAGAGTTAGCATCTTGGAAAGTGAAGTTGGAGGTCTGACGGCCCAGTTGTCTGCATATGCTCCTGCCATAGCCTCTTTGAGAGAGAATGTAAGTTCTCTTCAGCAAAATGCCCATCTTAGGAAGAAGCTTTTTGTGGAAAACAACCAGCAACACAAGGATGGCAAGCTGCCAAATAATCTATATCAGAGGAGCTCTCAAGATTACAAAGAAGACCAAAGCACTTTGATATCAAATGGAATTTCCGAATTGGAAGAAATGCAGACTATGATCAAAGAAGTTGAAAAGGCAGTTATTGAAGAAACGGAAAGGCTTTCAATTGAATTAGTTGAAAAGCAAATGGTGGAAGAAATTGAAAGGCTTACAACAAAGGAAAGGGCAGAGGCCTTGGTACAGACTGAAGTAAAATCAACAGGCAACTCGCATCAAGAAAATGGTAGCAAAGGAGAAGAGGTGGAACCTGTGAATGAGTACTCGGATGATAATCTGAAGTTGAGTAAACCTGAAAAGGCAGTTATTGAAGAAACGGAAAGGCTTTCAATTGAATTAGTTGAAAAGGAAATGGTGGAAGAAATTGAAAGGCTTACAACAAAGGAAAGGGCAGAGGCCTTGGTACAGACTGAAGAAGTAAAATCAACAGGCAACTCGCATCAAGAAAATGGTAGCGAAGGAGAAGAGGTGGAACCTGTGAATGAGTACTCGGATGATAATCTGAAGTTGAGTAAACCTGAAAAGGCAGTTATTGAAGATACGGAAAGGCTTTCAATTGAATTAGTTGAAAAGGAAATGGTGGAAGAAATTGAAAGGCTTACAACAAAGGAAAGGGCAGAGGCCTTGGTACAGACTGAAGTAAAATCAACAGGTAACTCAAATCAAGAAAATGGTAGCAAAGGAGAAGAGGTGGAACCTGTGAATGAGTACTCGGATGATAATCTGAAGTTGAGTAAACCTGAAATAAGGCTTTTGACGAAAGACATTCCGCTTGATCATGTTTCAGATTCTTCATTCTATGGAAGAAGCAGGAGAAAGAATGGTAGGGCTGATGATCAGATGCTCGAGTTATGGGAAGCAGCCGAGCAGCATTGCCTCCAGGATCCAGCAGCCAATATCAGGCAAGACCAGGCATCCCCACCAACTGAAGATGTTACCCCCTACCCTCGGTTTGCAAACTCACGGAGGACGATTCGAAAGTCTCCTTCAGAAGTTCAGGTTGAGAAGGAGTTGGGCATTGACAAGCTGGAGGTGTCCTACAGTATTCGGCAACCAAGTCATGATGGCAAGGAGGAAAAGATCTTGGAGAGGCTTGCTTCTGATGCTCAGAAACTGATGTGTCTACAAACGTCTGTGCAAGACTTGCATAAGAAGGTGGAGACTAACAAGAAGGGTAAGAAGATCAACAGCTCTGAATACGAAACGGTGAAAAGACAGTTGCACGAAGTTGAGGATGCGGTTGTGCAGCTGGTGGAAATTAATGATCAGTTGAAAAAGAATGTTGAAGAGTTTCCATCTTTGGATGAGCAGACTTCCATAGAGTTGGAGGAGGCTGGAAATGTCAGCAGAGAGGGAGTAACCGAACAAGCATGGAAAGGATCTGAGAAGATTGGAAGGTTGCAGTTTGAGTTGCAGAACATCCAATATGTTTTGGTGAAATGGGAGGGTGAAAAGAAAAGGAAAGGGAGGCATGGATTTTATGTGAGTAGAACAGGTCTTCTCCTGAGGGATTTTATTTACAGTGGCAGAAGTAGTAGTGAAAGGCGCAAGAAGGGTTGTTTGTGTGGTTGTATGAGACCATCAACGAGAGGAGAATAA

Protein Analysis

2128

Amino Acids

244.25

Weight (kDa)

4.91

Isoelectric Point (pI)

55.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 14 - 87 2.5e-38 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1806, 2624
AccB1I GGYRCC 1 cut(s) 1418
AccB7I CCANNNNNTGG 2 cut(s) 374, 4027
AccI GTMKAC 1 cut(s) 5911
AccIII TCCGGA 1 cut(s) 2327
AciI CCGC 7 cut(s) 584, 1364, 1465, 1576, 3584, 5558, 6026
AclI AACGTT 1 cut(s) 3593
AclWI GGATC 6 cut(s) 1999, 3111, 3375, 5666, 5679, 6175
AfiI CCNNNNNNNGG 8 cut(s) 374, 1906, 3412, 3433, 4027, 4698, 5688, 5736
AflII CTTAAG 3 cut(s) 539, 607, 3389
AflIII ACRYGT 2 cut(s) 546, 2806
AhlI ACTAGT 1 cut(s) 668
AjnI CCWGG 8 cut(s) 282, 693, 999, 1656, 2208, 2478, 5667, 5697
AjuI GAANNNNNNNTTGG 2 cut(s) 4590, 4622
AleI CACNNNNGTG 2 cut(s) 2438, 4008
AloI GAACNNNNNNTCC 2 cut(s) 485, 517
Alw21I GWGCWC 5 cut(s) 204, 1572, 2346, 3093, 4775
AlwI GGATC 6 cut(s) 1999, 3111, 3375, 5666, 5679, 6175
AlwNI CAGNNNCTG 6 cut(s) 374, 1070, 1462, 3073, 4195, 5902
Ama87I CYCGRG 3 cut(s) 197, 1023, 5633
Aor13HI TCCGGA 1 cut(s) 2327
AoxI GGCC 5 cut(s) 364, 4620, 4962, 5184, 5409
AseI ATTAAT 1 cut(s) 6048
Asp700I GAANNNNTTC 5 cut(s) 309, 3300, 3727, 4485, 6078
AspLEI GCGC 2 cut(s) 282, 6336
AspS9I GGNCC 5 cut(s) 365, 377, 697, 3403, 4621
AsuC2I CCSGG 2 cut(s) 1024, 1025
AsuHPI GGTGA 5 cut(s) 3328, 3611, 6007, 6235, 6248
AsuII TTCGAA 2 cut(s) 3589, 5764
AvaI CYCGRG 3 cut(s) 197, 1023, 5633
AvaII GGWCC 3 cut(s) 377, 697, 3403
AxyI CCTNAGG 2 cut(s) 352, 6293
BamHI GGATCC 1 cut(s) 5671
BanI GGYRCC 1 cut(s) 1418
BanII GRGCYC 4 cut(s) 204, 876, 2346, 4775
BarI GAAGNNNNNNTAC 2 cut(s) 4662, 4694
BbsI GAAGAC 7 cut(s) 953, 2064, 3293, 4164, 4383, 4797, 6278
Bbv12I GWGCWC 5 cut(s) 204, 1572, 2346, 3093, 4775
BbvCI CCTCAGC 1 cut(s) 4434
BceAI ACGGC 1 cut(s) 4635
BcgI CGANNNNNNTGC 4 cut(s) 389, 423, 1247, 1281
BciT130I CCWGG 8 cut(s) 284, 695, 1001, 1658, 2210, 2480, 5669, 5699
BciVI GTATCC 1 cut(s) 1315
BclI TGATCA 8 cut(s) 129, 259, 2838, 4063, 4848, 5563, 5623, 6052
BcnI CCSGG 2 cut(s) 1024, 1025
BcuI ACTAGT 1 cut(s) 668
BfaI CTAG 6 cut(s) 669, 996, 2030, 2601, 3221, 4322
BfmI CTRYAG 6 cut(s) 226, 749, 1610, 2660, 2870, 5827
BfrI CTTAAG 3 cut(s) 539, 607, 3389
BfuAI ACCTGC 2 cut(s) 1806, 2624
BfuI GTATCC 1 cut(s) 1315
BglII AGATCT 3 cut(s) 2733, 2761, 5868
BmcAI AGTACT 5 cut(s) 2513, 2994, 5051, 5276, 5498
Bme18I GGWCC 3 cut(s) 377, 697, 3403
BmeT110I CYCGRG 3 cut(s) 197, 1023, 5633
BmgT120I GGNCC 5 cut(s) 365, 377, 697, 3403, 4621
BmiI GGNNCC 7 cut(s) 502, 1420, 2572, 5037, 5262, 5484, 5673
BmrI ACTGGG 3 cut(s) 458, 2251, 4618
BmuI ACTGGG 3 cut(s) 458, 2251, 4618
BpiI GAAGAC 7 cut(s) 953, 2064, 3293, 4164, 4383, 4797, 6278
BplI GAGNNNNNCTC 8 cut(s) 444, 476, 1864, 1896, 2288, 2320, 4759, 4791
BpmI CTGGAG 9 cut(s) 888, 1022, 1190, 1868, 2144, 2192, 3974, 5651, 5837
Bpu10I CCTNAGC 1 cut(s) 4434
Bpu14I TTCGAA 2 cut(s) 3589, 5764
BpuEI CTTGAG 7 cut(s) 671, 840, 1172, 1338, 1344, 2885, 4761
BpuMI CCSGG 2 cut(s) 1024, 1025
Bsa29I ATCGAT 1 cut(s) 3977
BsaBI GATNNNNATC 5 cut(s) 2732, 2738, 5061, 5286, 5508
BsaI GGTCTC 3 cut(s) 998, 2946, 6358
BsaWI WCCGGW 1 cut(s) 2327
Bsc4I CCNNNNNNNGG 8 cut(s) 374, 1906, 3412, 3433, 4027, 4698, 5688, 5736
Bse1I ACTGG 9 cut(s) 216, 404, 453, 888, 2257, 2389, 3468, 3681, 4624
Bse21I CCTNAGG 2 cut(s) 352, 6293
Bse3DI GCAATG 7 cut(s) 132, 1151, 1882, 2306, 2653, 4417, 5659
Bse8I GATNNNNATC 5 cut(s) 2732, 2738, 5061, 5286, 5508
BseAI TCCGGA 1 cut(s) 2327
BseBI CCWGG 8 cut(s) 284, 695, 1001, 1658, 2210, 2480, 5669, 5699
BseCI ATCGAT 1 cut(s) 3977
BseJI GATNNNNATC 5 cut(s) 2732, 2738, 5061, 5286, 5508
BseLI CCNNNNNNNGG 8 cut(s) 374, 1906, 3412, 3433, 4027, 4698, 5688, 5736
BseMI GCAATG 7 cut(s) 132, 1151, 1882, 2306, 2653, 4417, 5659
BseNI ACTGG 9 cut(s) 216, 404, 453, 888, 2257, 2389, 3468, 3681, 4624
BseRI GAGGAG 8 cut(s) 233, 1862, 2477, 3074, 3391, 3836, 4783, 6131
BseYI CCCAGC 2 cut(s) 832, 2917
BsgI GTGCAG 4 cut(s) 2987, 3147, 3986, 6053
BshFI GGCC 5 cut(s) 366, 4622, 4964, 5186, 5411
BshNI GGYRCC 1 cut(s) 1418
BshVI ATCGAT 1 cut(s) 3977
BsiHKAI GWGCWC 5 cut(s) 204, 1572, 2346, 3093, 4775
BsiHKCI CYCGRG 3 cut(s) 197, 1023, 5633
BsiSI CCGG 2 cut(s) 1024, 2328
BslFI GGGAC 2 cut(s) 65, 471
BslI CCNNNNNNNGG 8 cut(s) 374, 1906, 3412, 3433, 4027, 4698, 5688, 5736
BsmBI CGTCTC 2 cut(s) 3218, 3484
BsmFI GGGAC 2 cut(s) 65, 471
BsmI GAATGC 5 cut(s) 1621, 1988, 2119, 2725, 3019
BsnI GGCC 5 cut(s) 366, 4622, 4964, 5186, 5411
Bso31I GGTCTC 3 cut(s) 998, 2946, 6358
BsoBI CYCGRG 3 cut(s) 197, 1023, 5633
Bsp119I TTCGAA 2 cut(s) 3589, 5764
Bsp1286I GDGCHC 6 cut(s) 204, 876, 1572, 2346, 3093, 4775
Bsp13I TCCGGA 1 cut(s) 2327
Bsp19I CCATGG 1 cut(s) 297
BspACI CCGC 7 cut(s) 584, 1364, 1465, 1576, 3584, 5558, 6026
BspANI GGCC 5 cut(s) 366, 4622, 4964, 5186, 5411
BspDI ATCGAT 1 cut(s) 3977
BspEI TCCGGA 1 cut(s) 2327
BspHI TCATGA 5 cut(s) 204, 616, 3454, 4504, 5848
BspLI GGNNCC 7 cut(s) 502, 1420, 2572, 5037, 5262, 5484, 5673
BspMAI CTGCAG 2 cut(s) 1614, 2664
BspMI ACCTGC 2 cut(s) 1806, 2624
BspPI GGATC 6 cut(s) 1999, 3111, 3375, 5666, 5679, 6175
BspQI GCTCTTC 2 cut(s) 1632, 2373
BspT104I TTCGAA 2 cut(s) 3589, 5764
BspT107I GGYRCC 1 cut(s) 1418
BspTI CTTAAG 3 cut(s) 539, 607, 3389
BspTNI GGTCTC 3 cut(s) 998, 2946, 6358
BsrDI GCAATG 7 cut(s) 132, 1151, 1882, 2306, 2653, 4417, 5659
BsrI ACTGG 9 cut(s) 216, 404, 453, 888, 2257, 2389, 3468, 3681, 4624
BssT1I CCWWGG 7 cut(s) 297, 928, 3360, 4050, 4965, 5187, 5412
Bst2UI CCWGG 8 cut(s) 284, 695, 1001, 1658, 2210, 2480, 5669, 5699
BstAFI CTTAAG 3 cut(s) 539, 607, 3389
BstAPI GCANNNNNTGC 3 cut(s) 1618, 3070, 5932
BstBI TTCGAA 2 cut(s) 3589, 5764
BstC8I GCNNGC 9 cut(s) 717, 1053, 1587, 2435, 2580, 2598, 3932, 4745, 5883
BstDSI CCRYGG 1 cut(s) 297
BstENI CCTNNNNNAGG 2 cut(s) 3410, 3431
BstHHI GCGC 2 cut(s) 282, 6336
BstNI CCWGG 8 cut(s) 284, 695, 1001, 1658, 2210, 2480, 5669, 5699
BstNSI RCATGY 4 cut(s) 550, 2284, 2582, 2810
BstSFI CTRYAG 6 cut(s) 226, 749, 1610, 2660, 2870, 5827
BstV2I GAAGAC 7 cut(s) 953, 2064, 3293, 4164, 4383, 4797, 6278
BstX2I RGATCY 7 cut(s) 2733, 2761, 3116, 3380, 5671, 5868, 6167
BstXI CCANNNNNNTGG 1 cut(s) 6112
BstYI RGATCY 7 cut(s) 2733, 2761, 3116, 3380, 5671, 5868, 6167
Bsu15I ATCGAT 1 cut(s) 3977
Bsu36I CCTNAGG 2 cut(s) 352, 6293
BsuI GTATCC 1 cut(s) 1315
BsuRI GGCC 5 cut(s) 366, 4622, 4964, 5186, 5411
BsuTUI ATCGAT 1 cut(s) 3977
BtgI CCRYGG 1 cut(s) 297
BtsIMutI CAGTG 6 cut(s) 446, 1250, 3093, 3461, 3688, 6316
BveI ACCTGC 2 cut(s) 1806, 2624
Cac8I GCNNGC 9 cut(s) 717, 1053, 1587, 2435, 2580, 2598, 3932, 4745, 5883
CaiI CAGNNNCTG 6 cut(s) 374, 1070, 1462, 3073, 4195, 5902
CciI TCATGA 5 cut(s) 204, 616, 3454, 4504, 5848
CfoI GCGC 2 cut(s) 282, 6336
Cfr13I GGNCC 5 cut(s) 365, 377, 697, 3403, 4621
Cfr9I CCCGGG 1 cut(s) 1023
ClaI ATCGAT 1 cut(s) 3977
CseI GACGC 3 cut(s) 39, 3235, 3338
CsiI ACCWGGT 1 cut(s) 693
CspCI CAANNNNNGTGG 4 cut(s) 2212, 2247, 4539, 4574
DraI TTTAAA 1 cut(s) 2494
Ecl136II GAGCTC 3 cut(s) 202, 2344, 4773
Eco130I CCWWGG 7 cut(s) 297, 928, 3360, 4050, 4965, 5187, 5412
Eco147I AGGCCT 3 cut(s) 4964, 5186, 5411
Eco24I GRGCYC 4 cut(s) 204, 876, 2346, 4775
Eco31I GGTCTC 3 cut(s) 998, 2946, 6358
Eco32I GATATC 4 cut(s) 993, 1161, 1937, 4809
Eco47I GGWCC 3 cut(s) 377, 697, 3403
Eco53kI GAGCTC 3 cut(s) 202, 2344, 4773
Eco81I CCTNAGG 2 cut(s) 352, 6293
Eco88I CYCGRG 3 cut(s) 197, 1023, 5633
EcoICRI GAGCTC 3 cut(s) 202, 2344, 4773
EcoNI CCTNNNNNAGG 2 cut(s) 3410, 3431
EcoO109I RGGNCCY 1 cut(s) 697
EcoRI GAATTC 3 cut(s) 1661, 2885, 3082
EcoRII CCWGG 8 cut(s) 282, 693, 999, 1656, 2208, 2478, 5667, 5697
EcoRV GATATC 4 cut(s) 993, 1161, 1937, 4809
EcoT14I CCWWGG 7 cut(s) 297, 928, 3360, 4050, 4965, 5187, 5412
EcoT38I GRGCYC 4 cut(s) 204, 876, 2346, 4775
ErhI CCWWGG 7 cut(s) 297, 928, 3360, 4050, 4965, 5187, 5412
Esp3I CGTCTC 2 cut(s) 3218, 3484
FaqI GGGAC 2 cut(s) 65, 471
FauI CCCGC 1 cut(s) 577
FauNDI CATATG 1 cut(s) 4636
FbaI TGATCA 8 cut(s) 129, 259, 2838, 4063, 4848, 5563, 5623, 6052
FblI GTMKAC 1 cut(s) 5911
FriOI GRGCYC 4 cut(s) 204, 876, 2346, 4775
FspBI CTAG 6 cut(s) 669, 996, 2030, 2601, 3221, 4322
GlaI GCGC 2 cut(s) 281, 6335
GsaI CCCAGC 2 cut(s) 836, 2921
GsuI CTGGAG 9 cut(s) 888, 1022, 1190, 1868, 2144, 2192, 3974, 5651, 5837
HaeIII GGCC 5 cut(s) 366, 4622, 4964, 5186, 5411
HapII CCGG 2 cut(s) 1024, 2328
HgaI GACGC 3 cut(s) 39, 3235, 3338
HhaI GCGC 2 cut(s) 282, 6336
Hin6I GCGC 2 cut(s) 280, 6334
HinP1I GCGC 2 cut(s) 280, 6334
HincII GTYRAC 1 cut(s) 666
HindII GTYRAC 1 cut(s) 666
HindIII AAGCTT 7 cut(s) 1647, 1856, 2288, 3395, 3773, 4142, 4706
HpaI GTTAAC 1 cut(s) 666
HpaII CCGG 2 cut(s) 1024, 2328
HphI GGTGA 5 cut(s) 3328, 3611, 6007, 6235, 6248
HpyCH4IV ACGT 3 cut(s) 3477, 3593, 5918
HpySE526I ACGT 3 cut(s) 3477, 3593, 5918
HspAI GCGC 2 cut(s) 280, 6334
Kpn2I TCCGGA 1 cut(s) 2327
Ksp22I TGATCA 8 cut(s) 129, 259, 2838, 4063, 4848, 5563, 5623, 6052
KspAI GTTAAC 1 cut(s) 666
LguI GCTCTTC 2 cut(s) 1632, 2373
MabI ACCWGGT 1 cut(s) 693
MaeI CTAG 6 cut(s) 669, 996, 2030, 2601, 3221, 4322
MaeII ACGT 3 cut(s) 3477, 3593, 5918
MaeIII GTNAC 9 cut(s) 12, 56, 642, 2391, 3250, 3683, 5441, 5722, 6145
MfeI CAATTG 8 cut(s) 3504, 3653, 3717, 4022, 4353, 4896, 5118, 5343
MflI RGATCY 7 cut(s) 2733, 2761, 3116, 3380, 5671, 5868, 6167
MhlI GDGCHC 6 cut(s) 204, 876, 1572, 2346, 3093, 4775
MmeI TCCRAC 9 cut(s) 528, 2829, 3225, 3639, 3910, 4008, 4069, 4588, 6093
MroI TCCGGA 1 cut(s) 2327
MroXI GAANNNNTTC 5 cut(s) 309, 3300, 3727, 4485, 6078
MslI CAYNNNNRTG 6 cut(s) 46, 441, 2438, 3639, 4008, 4737
MspA1I CMGCKG 1 cut(s) 6037
MspCI CTTAAG 3 cut(s) 539, 607, 3389
MspI CCGG 2 cut(s) 1024, 2328
MunI CAATTG 8 cut(s) 3504, 3653, 3717, 4022, 4353, 4896, 5118, 5343
Mva1269I GAATGC 5 cut(s) 1621, 1988, 2119, 2725, 3019
MvaI CCWGG 8 cut(s) 284, 695, 1001, 1658, 2210, 2480, 5669, 5699
NciI CCSGG 2 cut(s) 1024, 1025
NcoI CCATGG 1 cut(s) 297
NdeI CATATG 1 cut(s) 4636
NlaIV GGNNCC 7 cut(s) 502, 1420, 2572, 5037, 5262, 5484, 5673
NmeAIII GCCGAG 1 cut(s) 5677
NmuCI GTSAC 3 cut(s) 56, 3250, 3683
NspI RCATGY 4 cut(s) 550, 2284, 2582, 2810
NspV TTCGAA 2 cut(s) 3589, 5764
OliI CACNNNNGTG 2 cut(s) 2438, 4008
PaeI GCATGC 1 cut(s) 2582
PaeR7I CTCGAG 1 cut(s) 5633
PagI TCATGA 5 cut(s) 204, 616, 3454, 4504, 5848
PceI AGGCCT 3 cut(s) 4964, 5186, 5411
PciI ACATGT 2 cut(s) 546, 2806
PciSI GCTCTTC 2 cut(s) 1632, 2373
PcsI WCGNNNNNNNCGW 1 cut(s) 501
PctI GAATGC 5 cut(s) 1621, 1988, 2119, 2725, 3019
PdmI GAANNNNTTC 5 cut(s) 309, 3300, 3727, 4485, 6078
PflFI GACNNNGTC 1 cut(s) 788
PflMI CCANNNNNTGG 2 cut(s) 374, 4027
PfoI TCCNGGA 1 cut(s) 5667
PpuMI RGGWCCY 1 cut(s) 697
PscI ACATGT 2 cut(s) 546, 2806
PshBI ATTAAT 1 cut(s) 6048
Psp124BI GAGCTC 3 cut(s) 204, 2346, 4775
Psp1406I AACGTT 1 cut(s) 3593
Psp5II RGGWCCY 1 cut(s) 697
Psp6I CCWGG 8 cut(s) 282, 693, 999, 1656, 2208, 2478, 5667, 5697
PspFI CCCAGC 2 cut(s) 832, 2917
PspGI CCWGG 8 cut(s) 282, 693, 999, 1656, 2208, 2478, 5667, 5697
PspN4I GGNNCC 7 cut(s) 502, 1420, 2572, 5037, 5262, 5484, 5673
PspPI GGNCC 5 cut(s) 365, 377, 697, 3403, 4621
PspPPI RGGWCCY 1 cut(s) 697
PspXI VCTCGAGB 1 cut(s) 5633
PstI CTGCAG 2 cut(s) 1614, 2664
PstNI CAGNNNCTG 6 cut(s) 374, 1070, 1462, 3073, 4195, 5902
PsuI RGATCY 7 cut(s) 2733, 2761, 3116, 3380, 5671, 5868, 6167
PsyI GACNNNGTC 1 cut(s) 788
PvuII CAGCTG 1 cut(s) 6037
RseI CAYNNNNRTG 6 cut(s) 46, 441, 2438, 3639, 4008, 4737
SacI GAGCTC 3 cut(s) 204, 2346, 4775
SapI GCTCTTC 2 cut(s) 1632, 2373
Sau96I GGNCC 5 cut(s) 365, 377, 697, 3403, 4621
SbfI CCTGCAGG 1 cut(s) 2664
ScaI AGTACT 5 cut(s) 2513, 2994, 5051, 5276, 5498
SdaI CCTGCAGG 1 cut(s) 2664
SduI GDGCHC 6 cut(s) 204, 876, 1572, 2346, 3093, 4775
SexAI ACCWGGT 1 cut(s) 693
SfcI CTRYAG 6 cut(s) 226, 749, 1610, 2660, 2870, 5827
Sfr274I CTCGAG 1 cut(s) 5633
SfuI TTCGAA 2 cut(s) 3589, 5764
SinI GGWCC 3 cut(s) 377, 697, 3403
SlaI CTCGAG 1 cut(s) 5633
SmaI CCCGGG 1 cut(s) 1025
SmiMI CAYNNNNRTG 6 cut(s) 46, 441, 2438, 3639, 4008, 4737
SpeI ACTAGT 1 cut(s) 668
SphI GCATGC 1 cut(s) 2582
Sse8387I CCTGCAGG 1 cut(s) 2664
SseBI AGGCCT 3 cut(s) 4964, 5186, 5411
SsiI CCGC 7 cut(s) 584, 1364, 1465, 1576, 3584, 5558, 6026
SspI AATATT 1 cut(s) 3279
SspMI CTAG 6 cut(s) 669, 996, 2030, 2601, 3221, 4322
SstI GAGCTC 3 cut(s) 204, 2346, 4775
StuI AGGCCT 3 cut(s) 4964, 5186, 5411
StyI CCWWGG 7 cut(s) 297, 928, 3360, 4050, 4965, 5187, 5412
TaiI ACGT 3 cut(s) 3480, 3596, 5921
TaqI TCGA 8 cut(s) 292, 2295, 2346, 2952, 3589, 3977, 5634, 5764
TaqII GACCGA 1 cut(s) 2601
TatI WGTACW 9 cut(s) 180, 969, 1314, 1778, 2511, 2992, 5049, 5274, 5496
TscAI CASTG 6 cut(s) 453, 1257, 3093, 3468, 3688, 6316
TseFI GTSAC 3 cut(s) 56, 3250, 3683
Tsp45I GTSAC 3 cut(s) 56, 3250, 3683
TspGWI ACGGA 8 cut(s) 510, 4133, 4292, 4466, 4898, 5120, 5345, 5767
TspMI CCCGGG 1 cut(s) 1023
TspRI CASTG 6 cut(s) 453, 1257, 3093, 3468, 3688, 6316
Tth111I GACNNNGTC 1 cut(s) 788
Van91I CCANNNNNTGG 2 cut(s) 374, 4027
Vha464I CTTAAG 3 cut(s) 539, 607, 3389
VpaK11BI GGWCC 3 cut(s) 377, 697, 3403
VspI ATTAAT 1 cut(s) 6048
XagI CCTNNNNNAGG 2 cut(s) 3410, 3431
XbaI TCTAGA 2 cut(s) 3220, 4321
XceI RCATGY 4 cut(s) 550, 2284, 2582, 2810
XhoI CTCGAG 1 cut(s) 5633
XmaI CCCGGG 1 cut(s) 1023
XmiI GTMKAC 1 cut(s) 5911
XmnI GAANNNNTTC 5 cut(s) 309, 3300, 3727, 4485, 6078
XspI CTAG 6 cut(s) 669, 996, 2030, 2601, 3221, 4322
ZrmI AGTACT 5 cut(s) 2513, 2994, 5051, 5276, 5498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.